CN117607420B - Immunoassay system - Google Patents
Immunoassay system Download PDFInfo
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- CN117607420B CN117607420B CN202311682403.4A CN202311682403A CN117607420B CN 117607420 B CN117607420 B CN 117607420B CN 202311682403 A CN202311682403 A CN 202311682403A CN 117607420 B CN117607420 B CN 117607420B
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- 238000003018 immunoassay Methods 0.000 title claims abstract description 19
- 238000010438 heat treatment Methods 0.000 claims abstract description 45
- 239000010409 thin film Substances 0.000 claims abstract description 14
- 238000001514 detection method Methods 0.000 claims abstract description 13
- 238000004891 communication Methods 0.000 claims abstract description 12
- 239000010408 film Substances 0.000 claims abstract description 12
- 230000003287 optical effect Effects 0.000 claims abstract description 8
- 230000000149 penetrating effect Effects 0.000 claims abstract description 5
- 230000001737 promoting effect Effects 0.000 claims abstract description 3
- 238000003825 pressing Methods 0.000 claims description 22
- 239000002184 metal Substances 0.000 claims description 11
- 230000000903 blocking effect Effects 0.000 claims description 10
- 239000002390 adhesive tape Substances 0.000 claims description 8
- 230000006698 induction Effects 0.000 claims description 7
- 230000005540 biological transmission Effects 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 12
- 238000002474 experimental method Methods 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 description 22
- 102000004190 Enzymes Human genes 0.000 description 18
- 108090000790 Enzymes Proteins 0.000 description 18
- 239000000758 substrate Substances 0.000 description 16
- 239000007790 solid phase Substances 0.000 description 15
- 239000006249 magnetic particle Substances 0.000 description 11
- 238000000034 method Methods 0.000 description 8
- 239000002699 waste material Substances 0.000 description 8
- 239000000427 antigen Substances 0.000 description 7
- 102000036639 antigens Human genes 0.000 description 7
- 108091007433 antigens Proteins 0.000 description 7
- 238000004140 cleaning Methods 0.000 description 7
- BMXRLHMJGHJGLR-UHFFFAOYSA-N 1,2,4,5-tetrachloro-3-(2-chlorophenyl)benzene Chemical compound ClC1=CC=CC=C1C1=C(Cl)C(Cl)=CC(Cl)=C1Cl BMXRLHMJGHJGLR-UHFFFAOYSA-N 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 239000002775 capsule Substances 0.000 description 3
- 238000011897 real-time detection Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plasma & Fusion (AREA)
- Dispersion Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention belongs to the technical field of chemiluminescence immunoassay, and particularly relates to an immunoassay system, which comprises a base, wherein a drawer is connected above the base in a sliding manner, an actuator module is arranged above the base, and the drawer is positioned between the base and the actuator module; an integrated gas circuit board is arranged on the upper side of the actuator module, and a main board is arranged above the integrated gas circuit board; a heating support plate is arranged on the inner side of the drawer, an optical detection port is arranged on the upper side of the heating support plate in a penetrating way, and a thin film microfluidic chip is arranged on the upper side of the heating support plate; the lower side of the actuator module is movably provided with a pushing actuator for promoting the communication between adjacent chambers on the film microfluidic chip; all experimental steps of the device are completed by the executor in the totally-enclosed film micro-fluidic chip, so that the sample and the reagent can be prevented from being polluted, and the accuracy of the experiment is further ensured.
Description
Technical Field
The invention relates to the technical field of chemiluminescent immunoassay, in particular to an immunoassay system.
Background
Immunological detection is mainly a means for qualitatively or quantitatively detecting an antigen or an antibody in a sample by utilizing a specific reaction of the antigen and the antibody after in vitro combination. Common immunological techniques are radioimmunoassay, enzyme linked immunosorbent assay, fluorogenic immunoassay, chemiluminescent immunoassay.
The chemiluminescent immunoassay technology has the advantages of high accuracy, high sensitivity, short detection time and the like, and is widely applied. The existing chemiluminescent immunoassay analyzer (such as patent number 2011100604741) in the market is provided with a basic control system, a display system, an incubation module and a washing module, a movable sample adding module, a reagent area and a sample area, and the sample liquid and the reagent liquid are automatically distributed into a reaction cup through the movable module, so that the incubation, washing, detection and other procedures are further completed, and the chemiluminescent immunoassay analyzer can automatically detect conveniently.
However, the prior art has the following problems:
1. The reagent tube and the sample tube are moved by a turntable, a mechanical arm or a conveyor belt, and the reagent tube and the sample tube have complex structure and huge volume, are suitable for being used in a laboratory and are difficult to meet the current rapid real-time detection requirement;
2. Both the sample and the reagent are tested in an exposed environment and in a non-totally enclosed environment, with the risk of contamination of the sample and the reagent.
Therefore, we propose a full-automatic magnetic particle chemiluminescence immunoassay system based on a thin film microfluidic technology, which has compact structure, higher automation degree, no pollution, sample inlet and sample outlet, and is used for rapid real-time detection of samples.
Disclosure of Invention
The invention aims to provide an immunoassay system for solving the problems that the existing chemiluminescent immunoassay analyzer in the background art cannot detect rapidly in real time and samples and reagents are easy to pollute.
In order to achieve the above purpose, the present invention provides the following technical solutions: an immunoassay system comprises a base, wherein a drawer is connected above the base in a sliding manner, an actuator module is arranged above the base, and the drawer is positioned between the base and the actuator module; an integrated gas circuit board is arranged on the upper side of the actuator module, and a main board is arranged above the integrated gas circuit board;
A heating support plate is arranged on the inner side of the drawer, an optical detection port is arranged on the upper side of the heating support plate in a penetrating way, and a thin film microfluidic chip is arranged on the upper side of the heating support plate;
Air valves are arranged on the left side and the right side above the integrated air circuit board; the lower side of the actuator module is movably provided with a pushing actuator for promoting the communication of adjacent chambers on the film microfluidic chip and a cut-off actuator for blocking the communication of the adjacent chambers on the film microfluidic chip;
The lower side of the actuator module is provided with an inflation bin, the upper side of the inflation bin is connected with air valves through pipelines, the inflation bins are in one-to-one correspondence with the air valves, and the pipelines connected with the air valves are bonded on the inner side of the integrated air circuit board; the pushing type actuator and the cut-off type actuator comprise actuator pressing plates, O-shaped rings which are in sealing fit with the inflating bin are arranged on the outer sides of the actuator pressing plates, shoulder screws which are in sliding connection with the inflating bin are connected to the upper sides of the actuator pressing plates, and springs are connected between the shoulder screws and the cavity wall of the inflating bin.
Preferably, the heating support plate comprises a metal heating plate, a heat-conducting double-sided adhesive tape is arranged on the lower side of the metal heating plate, a heating PCB (printed circuit board) is arranged on the lower side of the heat-conducting double-sided adhesive tape, and a bottom shell is arranged on the lower side of the heating PCB; the thin film microfluidic chip is located on the upper side of the metal heating plate.
Preferably, a Hall sensor is arranged on the lower side of the heating PCB; the inner side of the actuator pressing plate is provided with induction magnets, and the induction magnets are in one-to-one correspondence with the Hall sensors.
Preferably, the air cylinder is installed to the upside of integrated gas circuit board, the output shaft of air cylinder runs through integrated gas circuit board and is connected with the lead screw connecting rod, the other end of lead screw connecting rod runs through the executor module and swing joint has the magnet seat, the downside of magnet seat is inlayed and is had back magnet.
Preferably, a motor is arranged on the upper side of the integrated gas circuit board, and a rotating shaft of the motor penetrates through the integrated gas circuit board and is connected with a main gear; the nut gear connected with the upper end of the magnet seat is sleeved on the outer side of the screw rod connecting rod; the lower side of the integrated gas circuit board is movably connected with a pinion, and the main gear and the nut gear are meshed with the pinion.
Preferably, a sliding groove matched with the nut gear and the magnet seat is formed in the lower side of the actuator module; and a transmission groove matched with the auxiliary gear and the main gear is formed in the upper side of the actuator module.
Compared with the prior art, the invention has the beneficial effects that:
1) During detection, the actuator pressing plate is pushed by air pressure to act, the pushing actuator extrudes a sac cavity which is in the same shape as the actuator pressing plate in the film microfluidic chip, samples or reagents are extruded into the other adjacent cavity from one cavity, liquid transfer, mixing, grabbing of antigens or antibodies by magnetic particles and the like are realized, and an optical detection module collects luminous signals generated when a substrate is combined with an enzyme-labeled antigen (antibody) -antibody (antigen) complex, so that quantitative detection of immune proteins is realized; because all experimental steps are completed by the executor in the fully-closed film micro-fluidic chip, the sample and the reagent can be prevented from being polluted, and the accuracy of the experiment is further ensured;
2) The device has small volume and convenient carrying, can be carried on a vehicle, and further meets the requirement of rapid and real-time detection;
3) The device adopts an integrated gas circuit board, so that the traditional complicated pipeline connection is avoided;
4) The magnetic actuator can be singly extended and retracted, can rotate in the extending process, can gather magnetic particles in the liquid extruding process, can drain liquid and simultaneously can enable most of the magnetic particles to be adsorbed on the magnet seat, so that the recovery efficiency of the magnetic particles can be improved;
5) The operating state of the executing device can be effectively monitored by feeding back the position of the pressing plate of the actuator through the Hall sensor.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic diagram of the motor and cylinder structure of the present invention;
FIG. 3 is a schematic view of an integrated gas circuit board structure according to the present invention;
FIG. 4 is a schematic diagram of the internal piping of the integrated gas circuit board according to the present invention;
FIG. 5 is a schematic top view of an actuator module according to the present invention;
FIG. 6 is a schematic view of the cross-sectional structure of the view A-A of FIG. 5 in accordance with the present invention;
FIG. 7 is a schematic view of a heating support plate structure according to the present invention;
Fig. 8 is a schematic diagram of a bottom view of a heating PCB board according to the present invention;
Fig. 9 is a two-dimensional structure diagram of a thin film microfluidic chip of the present invention.
In the figure: 10 main board;
20 motors, 21 main gears and 22 auxiliary gears;
30 cylinders, 31 back-attraction magnets, 32 magnet seats, 33 nut gears and 34 screw rod connecting rods;
The integrated gas circuit board 40 and the gas valve 41;
50 actuator modules, 561O rings, 562 springs, 563 shoulder screws, 564 induction magnets, 565 actuator press plates;
A 60 drawer;
70 an optical detection port;
An 80 base;
90 heating support plates, 91 metal heating plates, 92 heat-conducting double faced adhesive tape, 93 heating PCB plates, 94 bottom shells and 95 Hall sensors.
Description of the embodiments
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present invention and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Examples
Referring to fig. 1-9, the present invention provides a technical solution: an immunoassay system comprises a base 80, wherein a drawer 60 is slidably connected above the base 80, the drawer 60 can be pulled out of the base 80, a screw motor (not shown in the figure) is arranged above the inner side of the base 80, a film microfluidic chip with a sample is placed in a drawer of an instrument, and the drawer 60 is retracted by the screw motor; the actuator module 50 is installed above the base 80, after the drawer 60 is drawn back to the inner side of the base 80, the drawer 60 is located at the lower side of the actuator module 50, the actuator module 50 will perform various operations on the thin film microfluidic chip, and the experiment is completed in the thin film microfluidic chip in a totally enclosed manner, so that pollution or interference caused by human factors to the experimental result can not be generated. The inner side of the drawer 60 is provided with a heating support plate 90, the upper side of the heating support plate 90 is provided with an optical detection port 70 in a penetrating way, and the film microfluidic chip is arranged on the upper side of the heating support plate 90; the optical detection module is located below the optical detection port 70 and is used for quantitatively detecting a luminescent signal generated when the substrate is combined with the enzyme-labeled antigen (antibody) -antibody (antigen) complex.
The two-dimensional structure shape of the thin film microfluidic chip is shown in fig. 9, and the thin film microfluidic chip is composed of a plurality of capsule cavities, namely a cleaning liquid cavity 111, a substrate standby cavity 112, a substrate cavity 113, a solid phase carrier cavity 114, an enzyme cavity 115, an enzyme standby cavity 116, a sample cavity 117 and a waste liquid cavity 118; wherein the cleaning liquid cavity 111 is used for bearing cleaning liquid and is connected with the solid phase carrier cavity 114; the substrate standby cavity 112 and the substrate cavity 113 are used for bearing a substrate, the substrate standby cavity 112 is connected with the substrate cavity 113, and the substrate cavity 113 is connected with the solid-phase carrier cavity 114; the solid phase carrier chamber 114 is used for carrying solid phase carriers (magnetic particles) and is connected with the waste liquid chamber 118; the enzyme cavity 115 and the enzyme standby cavity 116 are used for bearing enzyme, the enzyme standby cavity 116 is connected with the enzyme cavity 115, and the enzyme cavity 115 is respectively connected with the solid phase carrier cavity 114 and the sample cavity 117; sample chamber 117 is for carrying a sample and waste chamber 118 is for carrying waste; the number of chambers varies somewhat according to the experimental project, wherein the enzyme, solid support, substrate and wash solution are pre-packaged in corresponding capsules of the chip. (the basic construction method of the thin film microfluidic chip adopts the prior art)
An integrated air circuit board 40 is arranged on the upper side of the actuator module 50, and a main board 10 is arranged above the integrated air circuit board 40; a push type actuator and a stop type actuator are mounted on the lower side of the actuator module 50; the pushing actuator has the function of extruding reagent liquid from one chamber of the film micro-fluidic chip to the other chamber; the cut-off type actuator is used for blocking communication between two adjacent chambers on the film micro-fluidic chip and blocking liquid flow between the two chambers; the lower side of the actuator module 50 is provided with an inflation bin, the upper side of the inflation bin is communicated with air valves 41 through pipelines, the number of the inflation bins is the same as that of the actuators, the inflation bins are in one-to-one correspondence, each inflation bin is respectively connected with one air valve 41, and a plurality of air valves 41 are respectively arranged on the left side and the right side above the integrated air circuit board 40; the pipeline connecting the inflating bin and the air valve 41 is bonded on the inner side of the integrated air circuit board 40, so that the traditional complicated pipeline connection is avoided. The push type actuator and the stop type actuator each comprise an O-ring 561, a spring 562, a shoulder screw 563 and an actuator pressure plate 565; an O-shaped ring 561 is sleeved on the outer side of the actuator pressing plate 565, and the outer side of the O-shaped ring 561 is in sealing fit with the inflation bin and is used for preventing air pressure in the inflation bin from leaking; the upper side of the actuator pressing plate 565 is connected with a shoulder screw 563, and the shoulder screw 563 is in sliding connection with the inflation bin and is used for guiding the actuator pressing plate 565 so that the actuator pressing plate 565 can only move up and down; a spring 562 is connected between the shoulder screw 563 and the wall of the inflatable cabin, the spring 562 is used for pushing the shoulder screw 563 to reset, the shoulder screw 563 drives the actuator pressing plates 565 to reset, and each actuator pressing plate 565 is provided with a capsule cavity corresponding to the shape of each actuator pressing plate 565 on the thin film microfluidic chip; the actuator is driven by the air valve 41, and the on-off of air pressure is controlled by the switch of the air valve 41, so that the extension or retraction of the actuator pressing plate 565 is controlled; the other end of the air valve 41 is connected with a high-pressure air tank through a pipeline, the air valve 41 is opened, air flow in the high-pressure air tank is introduced into the inflation bin, and the air pressure drives the actuator pressing plate 565 to extend out; after the air valve 41 is closed and released, the elastic force of the spring 562 pushes the actuator pressing plate 565 to return and retract through the shoulder screw 563.
The pushing type actuator comprises a pushing type actuator I511, a pushing type actuator II 521, a pushing type actuator III 530 and a pushing type actuator IV 542; the cut-off type actuator comprises a cut-off type actuator I512, a cut-off type actuator II 522, a cut-off type actuator III 541, a cut-off type actuator IV 543 and a cut-off type actuator V550. Wherein the pushing type actuator I511 is used for extruding the cleaning liquid in the cleaning liquid cavity 111 into the solid phase carrier cavity 114, and the stopping type actuator I512 is used for blocking the communication between the cleaning liquid cavity 111 and the solid phase carrier cavity 114; the number of the pushing type actuators II 521 is the same as the total number of the substrate standby cavity 112, the substrate cavity 113, the enzyme cavity 115 and the enzyme standby cavity 116, the pushing type actuators II 521 are in one-to-one correspondence with the cavities, and the pushing type actuators II 521 are used for extruding reagent liquid in the corresponding cavities into the cavities adjacent to and communicated with the corresponding cavities; the number of the cut-off type actuators II 522 is the same as the total number of the substrate standby cavity 112, the substrate cavity 113, the enzyme cavity 115 and the enzyme standby cavity 116, the cut-off type actuators II 522 are in one-to-one correspondence with the cavities, and the cut-off type actuators II 522 are used for blocking the communication between the adjacent cavities and blocking the communication between the substrate cavity 113 and the enzyme cavity 115 and the solid-phase carrier cavity 114; the pushing type actuator III 530 is used for extruding the waste liquid in the solid phase carrier cavity 114 to the waste liquid cavity 118, and the stopping type actuator V550 is used for blocking the communication between the solid phase carrier cavity 114 and the waste liquid cavity 118; the pushing type actuator IV 542 is used for pushing the sample in the sample cavity 117 into the enzyme cavity 115, the cut-off type actuator III 541 is used for blocking communication between the sample cavity 117 and the enzyme cavity 115, and the cut-off type actuator IV 543 is used for blocking communication between the sample cavity 117 and the outside.
The solid phase carrier cavity 114 is also provided with an independent magnetic actuator, the magnetic actuator is different from the push actuator and the stop actuator in structure, the magnetic actuator comprises an air cylinder 30 arranged on the upper side of the integrated air circuit board 40, through holes are formed in the upper sides of the integrated air circuit board 40 and the actuator module 50 in a penetrating way, an output shaft of the air cylinder 30 penetrates through the through holes of the integrated air circuit board 40 and is connected with a screw rod connecting rod 34, the other end of the screw rod connecting rod 34 penetrates through the through holes of the actuator module 50 and is movably connected with a magnet seat 32, a back attraction magnet 31 is inlaid on the lower side of the magnet seat 32, and the number of the back attraction magnets 31 is four; in the magnetic actuator, the air cylinder 30 pushes the magnet seat 32 to move downwards to extrude the solid-phase carrier cavity 114 through the screw rod connecting rod 34, so that the cleaning waste liquid in the solid-phase carrier cavity 114 is extruded, and meanwhile, the magnetic particles are adsorbed by the back-attraction magnet 31 in the extrusion process, so that the magnetic particles are recycled.
The upper side of the integrated gas circuit board 40 is provided with a motor 20, the upper side of the integrated gas circuit board 40 is also provided with a through hole for a motor rotating shaft to pass through, and the rotating shaft of the motor 20 penetrates through the through hole of the integrated gas circuit board 40 and is connected with a main gear 21; the outer side of the screw rod connecting rod 34 is movably sleeved with a nut gear 33, and the lower side of the nut gear 33 is connected with the upper end of the magnet seat 32; the lower side of the integrated gas circuit board 40 is movably connected with a secondary gear 22, and the primary gear 21 and the nut gear 33 are meshed with the secondary gear 22; the main gear 21 is driven to rotate by the motor 20, the main gear 21 drives the nut gear 33 to rotate by the auxiliary gear 22, the nut gear 33 drives the back attraction magnet 31 to rotate by the magnet seat 32, the back attraction magnet 31 rotates in the extending process, magnetic particles in the solid phase carrier cavity 114 can be gathered in the liquid extrusion process, most of the magnetic particles can be adsorbed on the magnet seat 32 while the liquid is discharged, and the recovery efficiency of the magnetic particles is improved.
The lower side of the actuator module 50 is provided with a chute which is matched with the nut gear 33 and the magnet seat 32; the upper side of the actuator module 50 is provided with a transmission groove which is matched with the auxiliary gear 22 and the main gear 21.
The heating support plate 90 comprises a metal heating plate 91, a heat-conducting double-sided adhesive tape 92 is arranged on the lower side of the metal heating plate 91, a heating PCB 93 is arranged on the lower side of the heat-conducting double-sided adhesive tape 92, and a bottom shell 94 is arranged on the lower side of the heating PCB 93; the thin film microfluidic chip is positioned on the upper side of the metal heating plate 91; the heating function is integrated on the PCB to form a heating PCB 93, and the traditional heating component is omitted by integrating the heating wire on the PCB; the heating PCB 93 and the metal heating plate 91 are bonded by using the heat-conducting double faced adhesive tape 92, so that the heating function can be realized, and the whole experimental process is kept at an optimal temperature.
A hall sensor 95 is arranged on the lower side of the heating PCB 93; the inner side of the actuator pressing plate 565 is provided with induction magnets 564, and the induction magnets 564 are in one-to-one correspondence with the hall sensors 95; when the actuator platen 565 is extended, the hall sensor may sense the magnet, thereby determining whether the actuator platen 565 is in an extended or retracted state.
While the fundamental and principal features of the invention and advantages of the invention have been shown and described, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but may be embodied in other specific forms without departing from the spirit or essential characteristics thereof; the present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. An immunoassay system comprising a base (80), characterized by: the upper part of the base (80) is connected with a drawer (60) in a sliding manner, an actuator module (50) is arranged above the base (80), and the drawer (60) is positioned between the base (80) and the actuator module (50); an integrated gas circuit board (40) is arranged on the upper side of the actuator module (50), and a main board (10) is arranged above the integrated gas circuit board (40);
A heating support plate (90) is arranged on the inner side of the drawer (60), an optical detection port (70) is formed in the upper side of the heating support plate (90) in a penetrating mode, and a thin-film microfluidic chip is arranged on the upper side of the heating support plate (90);
Air valves (41) are arranged on the left side and the right side above the integrated air circuit board (40); the lower side of the actuator module (50) is movably provided with a pushing actuator for promoting the communication of adjacent chambers on the film microfluidic chip and a cut-off actuator for blocking the communication of the adjacent chambers on the film microfluidic chip;
The lower side of the actuator module (50) is provided with an inflation bin, the upper side of the inflation bin is connected with the air valves (41) through pipelines, the inflation bin corresponds to the air valves (41) one by one, and the pipelines connected with the air valves (41) are bonded on the inner side of the integrated air circuit board (40); the pushing type actuator and the cut-off type actuator both comprise an actuator pressing plate (565), an O-shaped ring (561) which is in sealing fit with the inflating bin is arranged on the outer side of the actuator pressing plate (565), a shoulder screw (563) which is in sliding connection with the inflating bin is connected to the upper side of the actuator pressing plate (565), and a spring (562) is connected between the shoulder screw (563) and the cavity wall of the inflating bin;
The heating support plate (90) comprises a metal heating plate (91), a heat-conducting double-sided adhesive tape (92) is arranged on the lower side of the metal heating plate (91), a heating PCB (93) is arranged on the lower side of the heat-conducting double-sided adhesive tape (92), and a bottom shell (94) is arranged on the lower side of the heating PCB (93); the thin film micro-fluidic chip is positioned on the upper side of the metal heating plate (91);
The upper side of the integrated gas circuit board (40) is provided with a gas cylinder (30), an output shaft of the gas cylinder (30) penetrates through the integrated gas circuit board (40) and is connected with a screw rod connecting rod (34), the other end of the screw rod connecting rod (34) penetrates through an actuator module (50) and is movably connected with a magnet seat (32), and the lower side of the magnet seat (32) is inlaid with a back attraction magnet (31);
A motor (20) is arranged on the upper side of the integrated gas circuit board (40), and a rotating shaft of the motor (20) penetrates through the integrated gas circuit board (40) and is connected with a main gear (21); the outer side of the screw rod connecting rod (34) is sleeved with a nut gear (33) connected with the upper end of the magnet seat (32); the lower side of the integrated gas circuit board (40) is movably connected with a pinion (22), and the main gear (21) and the nut gear (33) are meshed with the pinion (22).
2. An immunoassay system according to claim 1, wherein: a Hall sensor (95) is arranged on the lower side of the heating PCB (93); an induction magnet (564) is arranged on the inner side of the actuator pressing plate (565), and the induction magnets (564) are in one-to-one correspondence with the Hall sensors (95).
3. An immunoassay system according to claim 1, wherein: a sliding groove matched with the nut gear (33) and the magnet seat (32) is formed in the lower side of the actuator module (50); and a transmission groove matched with the auxiliary gear (22) and the main gear (21) is formed in the upper side of the actuator module (50).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202311682403.4A CN117607420B (en) | 2023-12-08 | 2023-12-08 | Immunoassay system |
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| CN202311682403.4A CN117607420B (en) | 2023-12-08 | 2023-12-08 | Immunoassay system |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434330A (en) * | 2016-10-09 | 2017-02-22 | 戴敬 | Absolute quantification type digital nucleic acid analytic system based on efficient liquid drop microreactor |
| CN109536366A (en) * | 2018-11-29 | 2019-03-29 | 合肥中科易康达生物医学有限公司 | A kind of detection of nucleic acids micro-fluidic chip and nucleic acid detection system based on modified capillary |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110102355B (en) * | 2019-05-23 | 2021-04-27 | 江苏集萃智能传感技术研究所有限公司 | Microfluidic immunoassay chip and system |
| CN110927373B (en) * | 2019-11-26 | 2021-10-15 | 北京化工大学 | Full-automatic multi-index joint detection micro-fluidic chip and device |
| CN114100712A (en) * | 2021-11-19 | 2022-03-01 | 江苏液滴逻辑生物技术有限公司 | Hole liquid injection device of micro-fluidic chip, liquid injection method of hole liquid injection device and application of hole liquid injection device |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434330A (en) * | 2016-10-09 | 2017-02-22 | 戴敬 | Absolute quantification type digital nucleic acid analytic system based on efficient liquid drop microreactor |
| CN109536366A (en) * | 2018-11-29 | 2019-03-29 | 合肥中科易康达生物医学有限公司 | A kind of detection of nucleic acids micro-fluidic chip and nucleic acid detection system based on modified capillary |
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