CN117451580B - Analysis method of extrusion stability of drawn protein - Google Patents
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- 238000001125 extrusion Methods 0.000 title claims abstract description 61
- 108090000623 proteins and genes Proteins 0.000 title claims description 118
- 102000004169 proteins and genes Human genes 0.000 title claims description 118
- 238000004458 analytical method Methods 0.000 title description 13
- 238000000034 method Methods 0.000 claims abstract description 61
- 239000011148 porous material Substances 0.000 claims abstract description 48
- 102000034240 fibrous proteins Human genes 0.000 claims abstract 14
- 108091005899 fibrous proteins Proteins 0.000 claims abstract 14
- 238000004364 calculation method Methods 0.000 claims description 7
- 101710145505 Fiber protein Proteins 0.000 claims 7
- 229920006240 drawn fiber Polymers 0.000 claims 7
- 238000004519 manufacturing process Methods 0.000 abstract description 11
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 235000018102 proteins Nutrition 0.000 description 115
- 238000005491 wire drawing Methods 0.000 description 95
- 230000001953 sensory effect Effects 0.000 description 11
- 238000012360 testing method Methods 0.000 description 10
- 238000011156 evaluation Methods 0.000 description 8
- 238000012545 processing Methods 0.000 description 8
- 238000012512 characterization method Methods 0.000 description 4
- 238000005520 cutting process Methods 0.000 description 4
- 238000009826 distribution Methods 0.000 description 4
- 238000001035 drying Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 230000000007 visual effect Effects 0.000 description 3
- 108010064851 Plant Proteins Proteins 0.000 description 2
- 235000021118 plant-derived protein Nutrition 0.000 description 2
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- 238000007789 sealing Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000001087 myotubule Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000001007 puffing effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
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Abstract
本发明公开了一种拉丝蛋白挤压稳定性分析方法，包括获取第一工况和第二工况下挤压的拉丝蛋白的表观密度变异系数平均值、色值变异系数平均值的平均值、孔隙面积变异系数平均值的平均值、孔隙面积占比变异系数平均值的平均值，采用直接比较法或指标乘积法来综合判断第一工况和第二工况下拉丝蛋白的挤压相对稳定性。本发明具有的有益效果是，可对两种不同工况下挤压的拉丝蛋白的稳定性进行比较，得到稳定性较高的生产工况，对拉丝蛋白的生产具有重要的意义。
The present invention discloses a method for analyzing the extrusion stability of a fibrous protein, comprising obtaining the average value of the coefficient of variation of apparent density, the average value of the coefficient of variation of color value, the average value of the coefficient of variation of pore area, and the average value of the coefficient of variation of pore area ratio of fibrous protein extruded under the first working condition and the second working condition, and using a direct comparison method or an index product method to comprehensively judge the relative extrusion stability of the fibrous protein under the first working condition and the second working condition. The present invention has the beneficial effect that the stability of fibrous protein extruded under two different working conditions can be compared to obtain a production working condition with higher stability, which is of great significance to the production of fibrous protein.
Description
Technical Field
The invention relates to the field of judging the stability of wiredrawing proteins. More particularly, the invention relates to a method for analyzing extrusion stability of a drawn protein.
Background
The plant wiredrawing protein is fibrous plant protein with similar muscle fiber texture formed by the production and processing of plant protein by a special process. The wiredrawing protein needs extrusion in the processing process, and unstable phenomena such as deep and shallow color, expansion or larger or smaller color and the like often occur in the extrusion process of the wiredrawing protein, so that the stability of the quality of downstream products is further influenced. In particular, the stability of the extruded wire drawing proteins under different working conditions (such as different batches of raw materials, different extrusion devices and the like) may have obvious differences, so that in order to better evaluate the extrusion stability of the wire drawing proteins under different working conditions, the purposes of timely adjusting the working conditions with lower extrusion stability or screening the production working conditions with higher extrusion stability and the like are achieved, and a method capable of quantitatively characterizing the extrusion stability of the wire drawing proteins is needed to precisely characterize the stability of the wire drawing proteins in the extrusion and puffing process.
Disclosure of Invention
The invention aims to solve at least the problems and provide a method for analyzing extrusion stability of wiredrawing proteins, which can compare the stability of the quality of the wiredrawing proteins extruded under two different working conditions to obtain a production working condition with higher stability and has important significance on producing wiredrawing proteins with stable quality.
To achieve these objects and other advantages and in accordance with the purpose of the invention, as embodied and broadly described herein, there is provided a method for analyzing extrusion stability of a drawn protein, comprising the steps of:
s1, acquiring an index value representing extrusion stability of the wiredrawing protein under a single working condition:
a1, collecting N wiredrawing proteins in 1 minute on a wiredrawing protein extrusion line by taking 1 minute as a unit, and baking the wiredrawing proteins until the quality is basically unchanged, namely obtaining a sample;
a2, measuring the apparent density of each wiredrawing protein in the sample to obtain the apparent density variation coefficient of the sample;
a3, flattening each wiredrawing protein in the transverse cutting sample, exposing the transverse section of each wiredrawing protein, placing a graduated scale at the bottom of the transverse section, and shooting the transverse section to obtain a transverse section image of the wiredrawing protein with definite length;
a4, processing the cross section image to obtain a color value variation coefficient, a pore area variation coefficient and a pore area occupation ratio variation coefficient of each cross section, and further obtaining a color value variation coefficient average value, a pore area variation coefficient average value and a pore area occupation ratio variation coefficient average value of the sample;
a5, repeatedly collecting M minutes to obtain M samples, calculating the average value of each index of the M samples to obtain the average value of the apparent density variation coefficient, the average value of the color value variation coefficient, the average value of the pore area variation coefficient and the average value of the pore area ratio variation coefficient, and sequentially marking the average value as an A value, a B value, a C value and a D value; wherein the B value comprises an L value, an a value and a B value of the reaction color;
s2, judging extrusion stability of the wiredrawing protein under two different working conditions:
the two working conditions are a first working condition and a second working condition respectively, and the stability degree of the wiredrawing protein under the first working condition and the second working condition is judged by adopting a direct comparison method or a product method;
the direct comparison method comprises the following steps: directly comparing the six index values of the A value, the L value, the a value, the b value, the C value and the D value, and through statistical calculation, when at least one index value of the first working condition is obviously lower than the corresponding index value of the second working condition on the statistical 0.05 level, and the rest index values of the first working condition and the index values of the corresponding second working condition have no obvious difference on the statistical 0.05 level, indicating that the extrusion stability of the wiredrawing protein of the first working condition is higher than that of the wiredrawing protein of the second working condition;
when the six index values of the first working condition and the second working condition are not obviously different or obviously different in the level of 0.05 of statistics, but are obviously lower than the index value of the other side respectively in the first working condition and the second working condition, the direct comparison method cannot be adopted for judging, the product method is adopted for judging, and the product method is as follows: and calculating an index product value, wherein the index product value=C value×L value×a value×b value×E value×F value, and the smaller the value of the index product is, the higher the stability is.
Preferably, the resolution of the cross-section image is not lower than 2532×1170 pixels.
Preferably, in S1, N is 3 and M is 3.
Preferably, 1 protein is collected every 20s within 1 minute.
Preferably, in the step a4, the Image J software is used to process the cross-section Image, and the color value variation coefficient, the pore area variation coefficient and the pore area ratio variation coefficient of each cross section are calculated.
Preferably, in step a1, the harvested wire drawing protein is baked at 120 ℃ until the quality is substantially unchanged.
The invention at least comprises the following beneficial effects:
the average value of apparent density variation coefficient after extrusion of the wiredrawing protein and the average value of color value variation coefficient average value, average value of pore area variation coefficient average value and average value of pore area ratio variation coefficient average value of cross section after extrusion of the wiredrawing protein are calculated, and extrusion stability analysis of the wiredrawing protein under two different working conditions is carried out by a direct comparison method or a product method, so that the relative high and low of extrusion stability of the wiredrawing protein under two different working conditions can be judged, and the method has important significance for producing the wiredrawing protein with stable quality.
The extrusion stability analysis method of the wiredrawing protein can effectively characterize the extrusion stability of the wiredrawing protein, is particularly suitable for evaluating the extrusion stability of the wiredrawing protein under different working conditions, is further favorable for screening and obtaining production equipment or production conditions and the like with relatively high extrusion stability, and has important significance for extrusion production stability of the wiredrawing protein because the quantitative characterization is more accurate than the visual judgment or sensory evaluation, and the quantitative characterization can be realized.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Drawings
FIG. 1 is a graph of 9 baked drawn proteins from a first working condition to a third working condition in an embodiment of the present invention, wherein A represents the first working condition, B represents the second working condition, and C represents the third working condition;
FIG. 2 is a cross-sectional image of 9 wire drawing proteins for a first embodiment of the invention;
FIG. 3 is a graph of one of the drawn protein samples before background removal for the first operating mode in an embodiment of the present invention;
FIG. 4 is a graph of one of the drawn protein samples after background removal for the first operating mode in an embodiment of the present invention;
FIG. 5 shows the pore of one of the fiber-drawn proteins according to the first embodiment of the present invention.
Detailed Description
The present invention is described in further detail below with reference to examples to enable those skilled in the art to practice the same by referring to the description.
The invention provides a method for analyzing extrusion stability of wiredrawing protein, which specifically comprises the following steps:
s1, acquiring an index value representing extrusion stability of the wiredrawing protein under a single working condition:
a1, collecting N wiredrawing proteins in 1 minute on a wiredrawing protein extrusion line by taking 1 minute as a unit, and baking the wiredrawing proteins until the quality is basically unchanged, namely obtaining a sample;
a2, measuring the apparent density of each wiredrawing protein in the sample to obtain the apparent density variation coefficient of the sample;
a3, flattening each wiredrawing protein in the transverse cutting sample, exposing the transverse section of each wiredrawing protein, placing a graduated scale at the bottom of the transverse section, and shooting the transverse section to obtain a transverse section image of the wiredrawing protein with definite length;
a4, processing the cross section image to obtain a color value variation coefficient, a pore area variation coefficient and a pore area occupation ratio variation coefficient of each cross section, and further obtaining a color value variation coefficient average value, a pore area variation coefficient average value and a pore area occupation ratio variation coefficient average value of the sample;
a5, repeatedly collecting M minutes to obtain M samples, calculating the average value of each index of the M samples to obtain the average value of the apparent density variation coefficient, the average value of the color value Ping Bianyi coefficient, the average value of the pore area variation coefficient and the average value of the pore area ratio variation coefficient, and sequentially recording the average value as an A value, a B value, a C value and a D value; wherein the B value comprises an L value, an a value and a B value of the reaction color;
s2, judging extrusion stability of the wiredrawing protein under two different working conditions:
the two working conditions are a first working condition and a second working condition respectively, and the stability degree of the wiredrawing protein under the first working condition and the second working condition is judged by adopting a direct comparison method or a product method;
the direct comparison method comprises the following steps: directly comparing the six index values of the A value, the L value, the a value, the b value, the C value and the D value, and through statistical calculation, when at least one index value of the first working condition is obviously lower than the corresponding index value of the second working condition on the statistical 0.05 level, and the rest index values of the first working condition and the index values of the corresponding second working condition have no obvious difference on the statistical 0.05 level, indicating that the extrusion stability of the wiredrawing protein of the first working condition is higher than that of the wiredrawing protein of the second working condition;
when the direct comparison method cannot be adopted for judgment, that is, the six index values of the first working condition and the second working condition have no significant difference or have significant differences on the level of 0.05 in statistics, but the index values of the first working condition and the second working condition are respectively significantly lower than the index values of the other side, a product method is adopted for judgment, and the product method is as follows: calculating an index product value, wherein the index product value=c value×l value×a value×b value×e value×f value, and the smaller the value of the index product is, the higher the stability is;
in the above technical solution, N and M may be 3, 4, 5, etc., preferably a natural number of 3 or more, M minutes, which corresponds to M parallel tests; the method comprises the steps of baking the wiredrawing protein until the quality is basically unchanged, measuring the moisture similar to a drying method, removing the moisture of the wiredrawing protein, wherein the basically unchanged quality of the wiredrawing protein can be within a range allowed by measuring the moisture by referring to the drying method in the baking process, and when the wiredrawing protein is actually used, each wiredrawing protein sample needs to be stored in a sealing mode according to a sampling time sequence for later analysis; the measurement of the apparent density variation coefficient can be to measure the apparent density of each dried wire drawing protein by adopting a balance with a density assembly, and calculate the apparent density variation coefficient of N wire drawing proteins within 1 minute, wherein the calculation method of the variation coefficient is a calculation method known to a person skilled in the art, and excessive description is omitted here; transverse cutting is carried out on each dried wiredrawing protein, a graduated scale is placed below the wiredrawing proteins so as to determine the actual size of the wiredrawing proteins, meanwhile, a transverse section is photographed, a transverse section image is acquired, the resolution ratio of the acquired transverse section image is not lower than 2532 multiplied by 1170 pixels, and the illumination of the image acquisition environment is as consistent as possible so as to better analyze the image; the color value and the pore related index are obtained by processing the cross section Image through Image J software (National Institutes of Health, bethesda, USA), and the specific processing method is also a relatively mature mode; in actual use, M minutes are required to be collected, namely M parallel tests are carried out, M minutes are preferably continuously collected for M minutes, and the average value of the M parallel tests is taken to obtain the average value (A value) of the required apparent density variation coefficient, the average value (B value) of the color value variation coefficient, the average value (C value) of the pore area variation coefficient and the average value (D value) of the pore area ratio variation coefficient, wherein the color value (B value) comprises an L value, an a value and a B value which can react to color;
when judging extrusion stability of the wiredrawing proteins in the first working condition and the second working condition, judging that the smaller the index value of the six variation coefficients is, the higher the stability is, when comparing the extrusion stability of the wiredrawing proteins in the first working condition and the second working condition by adopting a direct comparison method, firstly calculating whether each index value in the first working condition and the second working condition has a statistically significant difference (the significance level is 0.05, when P is less than 0.05, the significance difference is shown), and if at least one index value in the first working condition is obviously lower than the index value in the corresponding second working condition on the statistically 0.05 level, and the rest index values have no statistically significant difference, indicating that the extrusion stability of the wiredrawing proteins in the first working condition is higher than that of the wiredrawing proteins in the second working condition; if there is no significant difference in statistics or there is significant difference in the six index values of the first working condition and the second working condition, but when at least one index value in each of the first working condition and the second working condition is significantly lower than the index value corresponding to the other, that is, when at least one index value in the first working condition is significantly lower than the index value corresponding to the second working condition and at least one index value in the second working condition is significantly lower than the index value corresponding to the first working condition, at this time, the direct comparison method cannot be used for judging, a multiplication method is adopted, namely, the six index values are multiplied to obtain a comprehensive judging value, that is, the larger the index product value is, and the extrusion stability of the wiredrawing protein is smaller; in actual use, the method can be used for comparison by a direct method or a product method, and if the direct comparison method cannot draw a conclusion, the method can be used for comparison and can be flexibly applied according to actual conditions.
In another technical scheme, the resolution ratio of the cross section image is not lower than 2532×1170 pixels, so that the acquired cross section image is clearer, and the subsequent data processing is convenient.
In another technical scheme, in S1, N is 3, M is 3, so that experimental experiments can be reduced as much as possible on the basis of guaranteeing statistical calculation significance of each index, and experimental efficiency is improved.
In another technical scheme, 1 wiredrawing protein is collected every 20s within 1 minute; the wiredrawing protein samples are collected in a mode of uniform time interval, and the collection mode is more uniform and reasonable.
In another technical scheme, in the step a4, image J software is utilized to process the cross section Image, the color value variation coefficient, the pore area variation coefficient and the pore area ratio variation coefficient of each cross section are obtained through calculation, and Image J software is utilized to process the cross section Image, so that the effect of processing the Image is good, and required data can be obtained more accurately and better.
In another technical scheme, in the step a1, the collected wiredrawing protein is baked at 120 ℃ until the quality is basically unchanged; the wiredrawing protein is baked at 120 ℃, so that the baking efficiency can be improved.
< example >
1. Obtaining an index value capable of judging extrusion stability of the wire drawing protein under a first working condition, and marking the wire drawing protein under the first working condition as a sample 1 (namely the following 9 wire drawing protein samples), wherein the method specifically comprises the following steps of:
s1, collecting one wire drawing protein every 20S on a wire drawing protein extrusion line in 1 minute, collecting 3 wire drawing proteins in 1 minute as a parallel test, continuously collecting 3 wire drawing proteins for 1 minute (namely 3 minutes), and obtaining 9 wire drawing proteins in total, wherein 3 repeated parallel tests are formed in 3 minutes; baking the collected wiredrawing proteins at 120 ℃ until the quality is basically unchanged, subpackaging the wiredrawing proteins according to a sampling time sequence, sealing and storing the wiredrawing proteins for subsequent treatment, wherein collected 9 baked wiredrawing proteins are shown in fig. 1, and A in fig. 1 represents a first working condition;
s2, apparent density data of the wiredrawing protein and a variation coefficient thereof are calculated: measuring the apparent density of each sample using a balance with a density assembly; calculating variation coefficients of apparent densities of 9 samples to obtain an average value of the variation coefficients of the apparent densities, and marking the average value as an A value; the results are shown in Table 3;
s3, horizontally and transversely cutting each wiredrawing protein, and exposing the transverse section of each wiredrawing protein; placing a graduated scale below the cross section, acquiring cross section images by adopting a camera, wherein the acquired cross section images have the respective rates of 2532 multiplied by 1170 pixels, and 9 cross section images are acquired in total, as shown in fig. 2;
s4, calculating the coefficient of variation of the cross section color value data: cross-section color value data were obtained by Image J software (National Institutes of Health, bethesda, USA): selecting a cross section to be analyzed, removing a region without data acquisition, converting an analysis image into RGB three channels, selecting a green channel, removing a background, and removing sample sections before and after the background are shown in figures 3 and 4; converting the original image into a Lab mode, introducing a selected pore section into the Lab mode, acquiring color value data of each pixel point on the cross section, and calculating a color value variation coefficient to obtain a color value variation coefficient of single wiredrawing protein, wherein the color value comprises an L value, an a value and a b value of the reaction color, and the specific result is shown in a table 1; calculating the average value of the color value variation coefficients of 3 samples within 1 minute to obtain the average value of the color value variation coefficients, calculating the average value and standard deviation of 3 parallel tests to obtain the average value of the color value variation coefficients, and recording the average value as an L value, an a value and a b value;
s5, calculating the variation coefficient of the cross section pore data: determining the actual size of unit pixels of an Image according to a graduated scale in the Image, selecting a cross section to be analyzed by utilizing an Image J software Lab mode, removing a region without data acquisition, and calculating the size and the area of the cross section according to the number and the distribution of pixel points in the cross section. Selecting a proper threshold to identify pore boundaries by using a threshold setting function of Image J software (the threshold can be selected by adopting a value automatically set by the software or can be manually finely adjusted to be determined by taking the identified pore boundaries as a standard), and outlining all pores as shown in FIG. 5; calculating the size and the area of the aperture according to the number and the distribution of the pixel points in the aperture; the partial data of the cross section analysis of one of the wiredrawing proteins is shown in table 2, and as the data of the cross section analysis of the wiredrawing proteins are more, only two lines of schematic diagrams are listed in table 2, and the pore area variation coefficient and the pore area occupation ratio variation coefficient of the single wiredrawing protein are calculated according to the analysis result, and the result is shown in table 3; calculating the average value of pore area variation coefficients and the average value of pore area ratio variation coefficients of 3 wiredrawing proteins within 1 minute, taking the average value of three parallel tests to obtain the average value of pore area variation coefficients, and marking the average value of pore area ratio variation coefficients as a C value and the average value of pore area ratio variation coefficients as a D value; the specific results are shown in Table 3;
2. according to the preparation steps of the sample 1, respectively obtaining drawn protein samples under a second working condition and a third working condition, respectively marking the drawn protein samples as a sample 2 and a sample 3, and according to the processing steps of the samples, obtaining six indexes of the sample 2 and the sample 3, which can judge the extrusion stability of the drawn protein, wherein the baked images of 9 drawn protein samples collected from the first working condition to the third working condition are shown in fig. 1, B in fig. 1 represents the second working condition, and C represents the third working condition; wherein, the statistical method comprises the following steps: the SPSS22.0 statistical software is adopted, the test level is significant with P < 0.05 as the difference, each group of data is expressed by adopting a mean value +/-standard deviation mode, the normal test and the variance alignment analysis are carried out, the single factor variance analysis method is adopted when the data obeys the normal distribution and the variance alignment, and the Kruskal-Wall test is adopted when the data does not obey the normal distribution or the variance alignment.
3. Sensory evaluation
And carrying out sensory evaluation on the samples 1-3 (after drying) to verify whether the judgment of the samples 1-3 is accurate or not. Sensory evaluation criteria and scoring criteria are shown in table 4.
4. Results and analysis
The results of the six index values of sample 1 and sample 2 are shown in Table 5, the results of the six index values of sample 1 and sample 3 are shown in Table 6, and the sensory evaluation results of samples 1 to 3 are shown in Table 7.
TABLE 1 coefficient of variation of color values for drawn protein sample 1
TABLE 2 partial data for analysis of cross section of one of the wire drawing proteins of sample 1
TABLE 3 index values of variation coefficients related to pore area, pore area ratio and apparent density of drawn protein sample 1
TABLE 4 sensory evaluation criteria for drawn protein samples
Table 5 six index numerical results table for sample 1 and sample 2 characterizing the extrusion stability of the wire drawing proteins
Note that: * Indicating that the index value of sample 1 has a significant difference (P < 0.05) from the index value of sample 2; no. indicates that the index value of sample 1 is not significantly different from the index value of sample 2
As can be seen from table 5, comparing the index of the color value variation data (L value, a value), the average value of the apparent density variation coefficient (a value) and the index of the pore area variation coefficient (C value), the drawn protein sample 1 and the drawn protein sample 2 have no significant difference (no significant difference at 0.05 level), and the drawn protein sample 2 has significant difference compared with the index of the sample 1 in terms of the index of the color value (b value) and the pore area ratio variation coefficient (D value), and the two index values of the sample 2 (b value, D value) are lower than the index value corresponding to the sample 1, indicating that the extrusion stability of the drawn protein of the sample 2 is higher than that of the sample 1; whereas from the visual observations of samples 1 and 2, and from the sensory scores of table 7, sample 2 did appear more uniform than sample 1, indicating that the drawn protein of sample 2 had relatively higher extrusion stability than sample 1.
Table 6 table of six index numerical values characterizing extrusion stability of the wire drawing proteins for sample 1 and sample 3
Note that: no. indicates that the index value of sample 1 is not significantly different from the index value of sample 3
As can be seen from table 6, the six indexes of sample 1 and sample 3 have no significant difference, and cannot be determined by a direct comparison method, and a product method is required. The index product value=l value×a value×b value×c value×d value×a value, the index product value of sample 3 is 8.3e+09, and the index product value of sample 1 is 2.5e+10, so that it can be known that the index product value of sample 3 is smaller than the index product value of sample 1, indicating that sample 3 has relatively high extrusion stability. From the sensory scores of table 7, sample 3 did have a more uniform appearance than sample 1.
Table 7 sensory scoring results of the drawn proteins of samples 1-3
From table 7, it can be seen that the sensory scores from high to low are sample 2, sample 3 and sample 1, respectively, which are the same as the evaluation results of the direct comparison method and the index product value method, and indicate that the method of the invention can effectively evaluate the extrusion stability of the wire drawing protein. In practical use, the index product value method is also applicable to the situation described by the direct judgment method.
In conclusion, the extrusion stability analysis method of the wiredrawing protein can effectively characterize the extrusion stability of the wiredrawing protein, is particularly suitable for evaluating the extrusion stability of the wiredrawing protein under different working conditions, is further beneficial to screening and obtaining production equipment or production conditions and the like with relatively high extrusion stability, and has important significance for extrusion production of the wiredrawing protein because the quantitative characterization is more accurate than visual judgment or sensory evaluation, and the quantitative characterization can be performed by one person.
Although embodiments of the present invention have been disclosed above, it is not limited to the details and embodiments shown, it is well suited to various fields of use for which the invention is suited, and further modifications may be readily made by one skilled in the art, and the invention is therefore not to be limited to the particular details and examples shown and described herein, without departing from the general concepts defined by the claims and the equivalents thereof.
Claims (6)
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