CN117210309A - Biological sample processing device and biological detection system - Google Patents

Abstract

The application provides a biological sample processing device and a biological detection system, and belongs to the technical field of biological sample detection. The biological sample processing device provided by the application comprises a sample processing component, a sample processing component and a biological sample processing component, wherein the sample processing component comprises a cracking cavity for receiving a sample to be processed and cracking the sample to be processed, a cleaning cavity for cleaning the sample to be processed, and a communication channel for communicating the cracking cavity and the cleaning cavity; the separation plate is arranged in the cracking cavity, the separation plate divides the cracking cavity into a first area and a second area, the bottom areas of the first area and the second area are communicated with each other, the corresponding areas of the separation plate are isolated from each other, and the first area is positioned between the communication channel and the second area. The biological sample processing device and the biological detection system at least solve the problems of high manufacturing cost and overlarge device volume of the biological sample processing device caused by the need of a large amount of lipophilic substances in the prior art.

Description

Biological sample processing device and biological detection system

Technical field

The present application belongs to the technical field of biological sample detection, and in particular relates to a biological sample processing device and a biological detection system.

Background technique

Nucleic acid testing has been widely used in many fields such as disease diagnosis and prognosis, public health, food safety, molecular breeding, and forensic identification. Nucleic acid testing mainly includes three steps: sample processing, nucleic acid extraction and purification, and nucleic acid amplification and detection. Nucleic acid testing in traditional laboratories requires dedicated operators and physical partitions, and many steps require manual operations. Not only does it cause cumbersome operations, but it also faces the risk that samples and reagents can easily cause harm to operators and contaminate nucleic acid products.

The fully integrated automated nucleic acid detection system can well solve the above problems and achieve the detection goal of "sample in - result out". It not only greatly reduces personnel operations, reduces potential contamination hazards of samples and amplification products, but also improves the reliability of detection. , save labor costs and reduce space occupation to the greatest extent.

Portable fully integrated nucleic acid testing, also known as "molecular diagnostic POCT (point-of-care testing, instant testing)", is a portable, miniaturized, automated nucleic acid testing system that uses external automation equipment to simulate manual operations to achieve sample processing to a fully automated process for nucleic acid detection.

Conventional molecular diagnostic POCT must at least be integrated and include multiple spaced sample processing chambers, extraction and purification chambers, and amplification detection chambers. Among them, the sample processing chamber is mainly used for lysis processing of samples, that is, to release DNA from cells, tissues, blood and other samples, and sometimes also includes the removal of inhibitors for subsequent PCR analysis; the extraction and purification chamber is used for nucleic acids Extraction and purification, which plays a key role in the PCR process, can effectively separate the target nucleic acid, remove contaminants, improve PCR reaction efficiency and reduce background interference, thereby ensuring the accuracy, sensitivity and specificity of the PCR reaction; amplification detection chamber , used to amplify the purified nucleic acid and detect the results.

Existing Technology In order to realize full automation of the detection process, the molecular diagnostic POCT device needs to move the substance to be detected between various chambers to realize the operation steps of different stages. Usually, magnetic particles are used to adsorb the substance to be detected, and then externally Magnetic field movement is used to realize the movement of magnetic particles that have captured the substance to be measured in the liquid in each chamber. However, this method is only at the theoretical stage. Actual products still need to consider the sealing issues between each chamber during transportation, as well as the sealing between each chamber. How to avoid the problem of magnetic particles not being lost during movement between chambers.

Contents of the invention

During the transportation of molecular diagnostic POCT consumable cartridges, and before the cartridges are placed in the instrument for operation, it is also necessary to prevent liquids from flowing between the chambers. For example, the lysate in the lysis chamber cannot enter the rear-end cleaning chamber. , for the same reason, the cleaning fluid in the cleaning chamber cannot flow into the cracking chamber. Therefore, a blocking device that can hinder the movement of liquid is required between different sample processing chambers. Filled paraffin is used as a valve. On the one hand, the paraffin can solidify to form a barrier for storage and transportation. On the other hand, paraffin is a lipophilic substance that floats on the liquid surface and does not participate in the reaction of nucleic acid detection. It also reduces the risk of magnetic particles during movement. The effect of friction with the supporting surface.

Paraffin can be liquefied at a lower temperature to cover the liquid surface of each chamber. The external magnetic field allows the magnetic particles to first pass through the liquid paraffin during the movement of each chamber, and the liquid paraffin layer needs to be thick enough to move in each chamber. As the magnetic particles pass through, the paraffin wax coats the magnetic particles to provide lubrication. For example, during the transfer process, magnetic particles used as extraction and purification reagents have a lubricating effect due to the lipophilic properties of liquefied paraffin, which can reduce the phenomenon of magnetic particles losing magnetism during transfer. That is, during the transfer process, the magnetic particles interact with the consumable cartridge. The friction or roughness of the inner surface decreases during the movement of magnetic particles, resulting in a decrease in the amount of substance to be measured that finally moves into the detection chamber, resulting in a significant reduction in the accuracy of the detection results.

The embodiments of this application provide a biological sample processing device and a biological detection system. The biological sample processing device and biological detection system provided by this application can be blocked by solid paraffin and can also use the lipophilicity of paraffin to reduce the movement process. friction in. In addition, in the process of using paraffin, the amount of paraffin used can be reduced, thereby reducing the time for solid paraffin to melt, thereby improving detection efficiency, reducing the manufacturing cost of the biological sample processing device, and miniaturizing the device.

A first aspect of the embodiment of the present application provides a biological sample processing device, including: a sample processing component; a lysis chamber for receiving and lysing a sample to be processed; and a cleaning chamber for cleaning the sample to be processed. ; And a communication channel connecting the cracking chamber and the cleaning chamber; the cracking chamber is used to accommodate an aqueous solution containing a sample to be processed; a partition is provided in the cracking chamber, and the partition separates the cracking chamber It is a first area and a second area in which the bottom areas are connected to each other and the corresponding areas of the partition are isolated from each other. The first area is located between the communication channel and the second area; the lysis chamber contains samples to be processed. When containing an aqueous solution, one end of the partition extends below the surface of the aqueous solution; between the first area and the end of the communication channel, there is also a lipophilic substance that can change its shape with temperature changes; so The lipophilic substance isolates the liquid flow of the cracking chamber and the cleaning chamber in a solid form at normal temperature; the lipophilic substance covers the aqueous solution in a liquid form, and the lipophilic substance is in contact with the aqueous solution. Aqueous solutions are immiscible.

Furthermore, the biological sample processing device further includes a magnetic conductive member, which is accommodated in at least one of the lysis chamber, the cleaning chamber and the communication channel. The magnetic conductive member is used for Under the action of an external magnetic field, it enters the cracking chamber and adsorbs the target substance in the sample to be processed.

Furthermore, the lipophilic substance is solid at the first temperature and is at least partially accommodated in the communication channel, and the magnetic conductive member is sealed in the lipophilic substance.

Furthermore, the lipophilic substance can melt at a second temperature to flow into the first area and float above the sample to be processed contained in the lysis chamber, and the second temperature is greater than the first temperature. temperature.

Further, the magnetically conductive member located in the communication channel can pass through the lipophilic substance into the lysis chamber under the action of an external magnetic field and contact the sample to be processed to capture the target substance, and pass through the The lipophilic substances are transferred to the cleaning chamber.

Further, the communication channel includes a first port adjacent to the lysis chamber and a second port adjacent to the cleaning chamber;

The magnetic conductive member is fixed on the first port or the second port through the lipophilic substance.

Further, the lysis chamber includes a chamber bottom wall and a plurality of chamber side walls formed by bending and extending from the chamber bottom wall. The chamber side walls include a third chamber spaced apart from the partition plate. A side wall of a region and a side wall of a second region, the side wall of the first region is adjacent to the communication channel, and the distance between the partition plate and the side wall of the first region is smaller than the distance between the side wall of the partition plate and the side wall of the first region. The spacing between the side walls of the second area.

Further, the communication channel, the lysis chamber and the cleaning chamber have openings in the same direction, and the sample addition component further includes a cover plate covering the openings;

The cover plate and the partition plate are spaced apart;

Alternatively, the cover plate is in contact with the partition plate.

Furthermore, the biological sample processing device further includes a sample adding member, which is at least partially inserted into the second area. The sample adding member has a receiving chamber for receiving the sample to be processed and a chamber communicating with the receiving chamber. cavity and the filter outlet of the second area.

Further, the sample processing component further includes an opening that communicates with the communication channel, the lysis chamber, and the cleaning chamber. The sample adding component further includes a cover plate covering the opening. The partition plate and the cover board connection.

Further, the cracking chamber includes a chamber bottom wall and a plurality of chamber side walls formed by bending and extending from the chamber bottom wall, and the partition passes through the chamber side walls in its width direction. Cooperate with surplus.

Further, the lysis chamber includes a chamber bottom wall, a plurality of chamber side walls formed by bending and extending from the chamber bottom wall, and guide rails extending inwardly from the chamber side walls, the The partition board is clamped on the guide rail.

Furthermore, cracking aids for crushing the sample to be processed are pre-stored in the cracking chamber.

Furthermore, a preset distance is provided between the bottom of the partition and the bottom of the cracking chamber, so that the bottom areas of the first region and the second region are connected to each other.

Further, a biological detection system includes: a biological sample processing device; and

Magnet, abutted against the side wall of the outer chamber of the sample processing component and used to provide an external magnetic field; and/or

a heating device, which is in contact with the communication channel and/or the outer chamber side wall of the cleaning chamber, and is used for heating; and/or an ultrasonic device, which is in contact with the outer chamber side wall of the cracking chamber, and is used for heating Ultrasonic waves are emitted to the lysis chamber and/or the communication channel.

The technical solutions provided by the embodiments of the present application at least bring the following beneficial effects:

The present application provides a biological sample processing device. In the biological sample processing device, partitions are provided in the lysis chamber, and the partitions are used to divide the lysis chamber into a third area in which the bottom areas are connected to each other and the areas corresponding to the partitions are isolated from each other. The first area and the second area ensure that the paraffin that plays the role of isolation does not need to cover the liquid surface of the entire cracking chamber, but only needs to cover the liquid surface of the first area. In this way, while ensuring the required thickness of the paraffin layer, it is reasonable. The amount of paraffin used is reduced, thereby reducing the size of the cartridge and achieving product miniaturization. At the same time, the amount of solid paraffin used in the blocking process is reduced. During the detection process, high temperature is used to melt the paraffin to form liquid paraffin, thereby reducing the pre-processing time and improving the detection speed of the biological sample processing device.

This application also provides a biological detection system. Since the biological detection system includes the biological sample processing device of the aforementioned technical solution, the biological detection system can also reduce manufacturing costs and miniaturize the device.

It should be understood that the above general description and the following detailed description are only exemplary and explanatory, and do not limit the present application.

Description of the drawings

In order to explain the technical solutions of the embodiments of the present application more clearly, the drawings required to be used in the embodiments of the present application will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. Those of ordinary skill in the art can also obtain other drawings based on these drawings without exerting creative efforts.

Figure 1 is a schematic three-dimensional structural diagram of a biological sample processing device provided by some embodiments of the present application;

Figure 2 is a cross-sectional view along line A-A of the biological sample processing device shown in Figure 1;

Figure 3 is an enlarged view of part B of the biological sample processing device shown in Figure 2;

Figure 4 is a schematic three-dimensional structural diagram of a sample processing component in a biological sample processing device provided by some embodiments of the present application;

Figure 5 is a schematic three-dimensional structural diagram of the sample addition component in the biological sample processing device provided by some embodiments of the present application;

Figure 6 is a schematic diagram of the coverage of lipophilic substances in the prior art;

Figure 7 is a schematic diagram of the coverage of lipophilic substances in this embodiment.

Among them, the reference marks are:

100. Biological sample processing device; 10. Sample processing component; 102. First area; 103. Second area; 11. Lysis chamber; 111. Chamber bottom wall; 112. Chamber side wall; 1121. First area side wall; 1122, second area side wall; 12, cleaning chamber; 13, communication channel; 131, first port; 132, second port; 14, opening; 20, partition; 30, sample addition component; 31, containment Chamber; 32. Filter outlet; 33. Cover plate; 40. Lipophilic substance; 50. Magnetic conductive piece; 300. Paraffin; 400. Sample to be processed.

Detailed ways

Features and exemplary embodiments of various aspects of the application are described in detail below. In the following detailed description, numerous specific details are set forth in order to provide a thorough understanding of the application. However, it will be apparent to one skilled in the art that embodiments of the present application may be practiced without some of these specific details. The following description of embodiments is merely intended to provide a better understanding of the present application by illustrating examples of the present application.

In the description of this application, it should be understood that the orientation descriptions, such as the orientation or positional relationship indicated by up, down, back, etc., are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the implementation of this application. Examples and simplified descriptions are not intended to indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be construed as limiting the embodiments of the present application.

In the description of the embodiments of this application, the meaning of multiple is two or more, and greater than, less than, etc. are understood as excluding the original number. If there is a description of first and second, it is only for the purpose of distinguishing technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of indicated technical features or implicitly indicating the order of indicated technical features. relation.

In the description of the embodiments of this application, unless otherwise explicitly limited, words such as setting, installation, and connection should be understood in a broad sense. Those skilled in the art can reasonably determine the specific meaning of the above words in this application based on the specific content of the technical solution. .

It should be noted that, as long as there is no conflict, the embodiments and features in the embodiments of this application can be combined with each other. The embodiments will be described in detail below with reference to the accompanying drawings.

Molecular diagnostic POCT is a testing method that uses portable analytical instruments and supporting reagents to quickly obtain test results at the sampling site. Its main criterion is that it does not require a fixed testing location. The reagents and instruments are portable and can be used. Operate promptly.

In this regard, biological sample processing devices are usually needed at the testing site to perform operations such as lysis, cleaning, amplification, etc. on the collected biological samples, so that the portable analysis instruments on site can accurately identify the collected biological samples. information, and then make corresponding judgments. However, in the existing technology, the lysis step is usually performed in different instruments from the cleaning and amplification steps, resulting in the inability to truly achieve the real-time detection effect of sample input and result output.

Molecular diagnostic POCT integrates lysis, cleaning, amplification, and detection in different chambers of the same consumable cartridge. Paraffins in different sample processing chambers are usually connected to each other to form wax channels. The design of this wax channel often requires a large amount of paraffin in order to achieve sealing during solidification, communication and lubrication during liquefaction. However, there are some problems with large amounts of paraffin: Since a large amount of paraffin is in a solid state during transportation or storage, the solid state paraffin needs to be liquefied during the detection process, and it takes more time for a large amount of paraffin to change from solid to liquid. This is contrary to the rapid detection pursued by molecular diagnostic POCT. Therefore, how to provide a biological sample processing device that reduces the amount of paraffin while minimizing the product is an urgent problem that those skilled in the art need to solve. Since the biological sample processing device needs to complete at least some of the above steps, the biological sample processing device usually includes a plurality of sample processing chambers arranged at intervals and a connecting channel for connecting adjacent sample processing chambers. At the same time, the connecting channel is usually And/or lipophilic substances are placed in the sample processing chamber to isolate different detection solutions in different sample processing chambers (the detection solution can be samples to be processed, cleaning solutions, amplification reagents, etc.).

If the lysis step is to be built into a biological sample processing device, a larger volume of sample to be processed (the sample to be processed is mixed with the sample collected from the organism and the lysate) needs to be added to the sample processing chamber, causing the sample processing The larger volume of the cavity, coupled with height restrictions, results in a larger cross-sectional area of the sample processing cavity, which leads to a greater demand for lipophilic substances covering the sample processing cavity, further increasing the volume of the biological sample processing device. Not conducive to miniaturization.

As mentioned above, since the capacity of each sample processing chamber is large or small, in some larger sample processing chambers, it is usually necessary to cover the entire surface of the sample processing chamber with lipophilic substances. This will increase the lipophilicity on the one hand. The amount of substances used leads to an increase in cost; on the other hand, due to the increase in lipophilic substances, correspondingly, it is necessary to increase the size of the communication channel for accommodating lipophilic substances.

Based on the above considerations, in order to avoid the increase in the amount of lipophilic substances caused by the excessive volume of individual sample processing chambers, the inventors designed a sample processing device after in-depth research. By setting partitions in the lysis chamber, the lipophilic substances are The substance only needs to cover the surface of the first area, thereby solving the problem of excessive dosage of lipophilic substances and excessive volume of the sample processing device.

The biological sample processing device and biological detection system provided by this application can be applied to clinical testing in hospitals, rapid testing in private clinics, home self-testing, testing outside the laboratory, decentralized testing, etc. This application uses a biological sample processing device. Take on-site nucleic acid testing as an example to illustrate.

The technical solutions of the biological sample processing device and biological detection system provided by the specific embodiments of the present application will be further described below.

Figure 1 is a schematic three-dimensional structural diagram of a biological sample processing device provided by some embodiments of the present application; Figure 2 is a cross-sectional view of the biological sample processing device shown in Figure 1 along line A-A; Figure 3 is a schematic view of the biological sample processing device shown in Figure 2 An enlarged view of Part B; Figure 4 is a schematic three-dimensional structural view of the sample processing component in the biological sample processing device provided by some embodiments of the present application; Figure 5 is a schematic three-dimensional structural view of the sample adding component in the biological sample processing device provided by some embodiments of the present application. .

Please refer to FIGS. 1 to 5 . In a first aspect, an embodiment of the present application provides a biological sample processing device 100 , that is, a consumable cartridge, which includes a sample processing component 10 and a partition 20 . The sample processing component 10 includes a lysis chamber 11 for receiving and lysing the sample to be processed, a cleaning chamber 12 for cleaning the sample to be processed, and a communication channel 13 connecting the lysis chamber 11 and the cleaning chamber 12; The partition 20 is arranged in the cracking chamber 11. The partition 20 divides the cracking chamber 11 into a first area 102 and a second area 103 in which the bottom areas are connected to each other and the areas corresponding to the partition 20 are isolated from each other. The first area 102 Located between the communication channel 13 and the second area 103 .

In some embodiments of the present application, the biological sample processing device 100 can be a consumable and used to detect biological samples in cooperation with other functional instruments and analytical instruments; in other embodiments of the present application, the biological sample processing device 100 100 can also be a recyclable product. After a single use, the biological sample processing device 100 can be reused by cleaning, disinfecting, re-injecting the detection solution and packaging.

The biological sample processing device 100 can be made of transparent materials such as plastic and glass to facilitate operation and observation by medical staff.

The sample processing component 10 has at least a lysis chamber, a cleaning chamber, and an amplification chamber.

Among them, the lysis chamber is used to cleave the sample to be processed that enters it to release the target substance in the sample to be processed. In embodiments, the target substance is nucleic acid, and the function of the lysis chamber is to destroy the sample and release the nucleic acid in the sample into the lysis chamber.

The target substance can be released by physical release. For example, in some embodiments of the present application, a certain number of lysis particles can be encapsulated in the lysis chamber in advance. When the sample to be processed enters the lysis chamber, the function can be used. The instrument drives the lysis particles to move within the lysis chamber, grinding the sample and releasing the target substances.

In these embodiments of the present application, the pyrolysis particles can be polystyrene particles, borosilicate glass particles, quartz glass particles, etc.; the functional instrument can select an ultrasonic device, and by using the ultrasonic device to release ultrasonic waves, the pyrolysis particles can generate rapid Vibrate to grind the sample to be processed and better release the target substance.

The cleaning fluid is stored in the cleaning chamber and is used to clean away other substances besides the target substance, such as inhibitors and lysis fluid in the lysis chamber, to improve the accuracy of detection.

The amplification chamber stores freeze-dried reaction components and complex solutions of bioassays, and is used to amplify target substances to increase their quantity for monitoring and analysis.

The lysis chamber 11 and the cleaning chamber 12 may be any two adjacent chambers among the lysis chamber, the cleaning chamber, the amplification chamber and other unmentioned chambers. At the same time, this application does not limit the size, side wall shape, and bottom wall shape of each sample processing chamber, and can be selected according to actual conditions.

For example, the volume of each sample processing chamber can be determined according to the amount of detection liquid that needs to be stored, or according to whether other structures need to be added to the chamber; in order to facilitate the transportation of materials in the sample processing chamber, side walls can be provided and the bottom wall are provided with rounded corners; in order to facilitate the placement of the biological sample processing device 100, the bottom walls of multiple sample processing chambers can also be arranged on the same plane or different planes, etc.

The lysis chamber 11 is used to receive samples to be processed. A possible implementation is that the lysis chamber 11 can be used to receive samples to be processed that enter from the outside, or to receive samples to be processed that have been processed by other sample processing chambers.

The partition 20 is disposed in the cracking chamber 11. A possible implementation is that the partition 20 can be directly formed in the cracking chamber 11, directly dividing the cracking chamber 11 into a first layer where the bottom areas are interconnected and the corresponding areas of the partition 20 are isolated from each other. Area 102 and second area 103;

It can also be detachably connected to and closely matched with the cracking chamber 11, and the cracking chamber 11 is divided into a first area 102 and a second area 103 with the bottom areas connected to each other and the corresponding areas of the partition 20 isolated from each other. It can be based on the actual production. Choose according to the situation.

The bottom areas of the first area 102 and the second area 103 are connected to each other. A possible implementation is that there is at least a partial structure at the bottom of the partition 20 so that the first area 102 and the second area 103 are connected. For example, the bottom end of the partition 20 can be provided with a through hole connecting the first region 102 and the second region 103. The first region 102 and the second region 103 are connected through the through hole and can exchange materials.

In these embodiments of the present application, by arranging the bottom areas of the first area 102 and the second area 103 to communicate with each other, the detection liquid in the lysis chamber 11 communicates with each other in the first area 102 and the second area 103 to ensure lysis. Cavity 11 functions.

The first area 102 is located between the communication channel 13 and the second area 103, which can effectively reduce the sealing area of the cracking chamber 11.

For example, taking the cracking chamber 11 as the cracking chamber and the cleaning chamber 12 as the cleaning chamber, the partition 20 separates the cracking chamber into a first region 102 and a second region 103 .

In order to prevent the detection liquid pre-stored in the lysis chamber from flowing into the cleaning chamber through the connecting channel 13, a layer of lipophilic material needs to be laid on the upper surface of the lysis chamber to prevent the detection liquid in the lysis chamber from entering the cleaning chamber through the connecting channel 13, so , by arranging the partition 20 in the cracking chamber and dividing the cracking chamber into the first area 102 and the second area 103, the lipophilic substance only needs to be laid on the upper surface of the first area 102 connected with the communication channel 13. The use of lipophilic substances is reduced to a great extent and the manufacturing cost is reduced. At the same time, since the amount of lipophilic substances used is reduced, the space of the communication channel 13 can be reduced, further achieving miniaturization.

It should be noted that the movement of the sample to be processed in the cracking chamber 11, the cleaning chamber 12 and the connecting channel 13 requires the help of other components. After using other components to adsorb the sample to be processed, the process to be processed is achieved by moving other components. Movement of samples. In this way, on the one hand, the role of the lipophilic substance is to form a liquid environment in the communication channel 13 and each sample processing chamber, so that other components can move smoothly in each sample processing chamber or communication channel 13 without being affected by friction. It sticks to the inner wall of the sample processing chamber or the communication channel 13; on the other hand, it serves to separate the detection liquid in each sample processing chamber.

In other words, the setting of lipophilic substances can optimize the transfer efficiency of samples to be processed while avoiding the flow of detection liquid in each sample processing chamber.

In some embodiments of the present application, if necessary, the partition 20 can be disposed in the lysis chamber 11, the cleaning chamber 12 and other sample processing chambers at the same time to further streamline the amount of lipophilic substances, thereby making each sample processing chamber compact. , and the biological sample processing device 100 is miniaturized.

In the biological sample processing device 100 provided in the above embodiments of the present application, the partition 20 is provided in the lysis chamber 11 and the partition 20 is used to divide the lysis chamber 11 into interconnected bottom areas and isolated areas corresponding to the partition 20 The first region 102 and the second region 103 make it unnecessary for the lipophilic substance that plays a role in isolation to cover the entire surface of the lysis chamber 11, but only needs to cover the surface of the first region 102. In this way, the lipophilic substance is greatly reduced. The dosage of lipid substances reduces the manufacturing cost. At the same time, since the amount of lipophilic substances used is reduced, the space of the communication channel 13 can be reduced, further achieving miniaturization.

In some embodiments of the present application, the biological sample processing device 100 further includes a sampling member 30, which is at least partially inserted into the second region 103. The sampling member 30 has a receiving chamber 31 for receiving the sample to be processed and a communication The receiving cavity 31 and the filter outlet 32 of the second area 103 are included.

The sample adding member 30 is used to add the sample to be processed. In some embodiments of the present application, the sampling component 30 can be used to add the sample to be processed collected by the external component into the second area 103 of the lysis chamber; it can also be used to add the sample to be processed that has been lysed by the external component. Cleaning chamber; it can also be used to add samples to be processed that have been cleaned by external components into the amplification chamber.

The sample adding member 30 is at least partially inserted into the second region 103. A possible implementation is that the sample adding member 30 is at least partially disposed in the second region 103, where the sample adding member 30 can be detachably connected or fixedly connected with the lysis chamber 11. It is even molded in one piece and can be selected according to the actual situation.

The holding chamber 31 is used to hold the sample to be processed, and the filter outlet 32 communicates with the holding chamber 31 and the second area 103 . For example, in an embodiment in which the sample adding member 30 is fixedly connected to the lysis chamber 11, the receiving chamber 31 may include a feeding port (not shown), and the sample to be processed may be added into the receiving chamber 31 through the feeding port; when adding the sample In the embodiment in which the component 30 is detachably connected to the lysis chamber 11, in addition to the aforementioned arrangement method, the filter port 32 can also be detachably provided. During the sample addition stage, the receiving chamber 31 is exposed by disassembling the filter port 32. After adding the sample to be processed, install the filter port 32.

The arrangement of the sample adding component 30 allows the sample to be processed to be filtered before entering the lysis chamber 11 to remove unnecessary substances. At the same time, the lysis chamber 11 needs to be designed larger to accommodate the sample adding component 30 . Therefore, in these embodiments of the present application, the amount of lipophilic substances is reduced by arranging the partition 20 in the lysis chamber 11 so that the partition 20 is used to separate the lysis chamber 11 .

In some embodiments of the present application, the biological sample processing device 100 further includes a lipophilic substance 40 used to form a barrier between the lysis chamber 11 and the cleaning chamber 12 . The lipophilic substance 40 and the to-be-processed substance contained in the lysis chamber 11 The samples are immiscible with each other.

The lipophilic substance 40 and the sample to be processed contained in the lysis chamber 11 are incompatible with each other. A possible implementation is that the lipophilic substance 40 will not have any chemical reaction with the sample to be processed and the detection solution contained in the lysis chamber 11 , and the lipophilic substance 40 will be stratified with the detection liquid, that is, the lipophilic substance 40 floats on the surface of the detection liquid, and will not form an emulsion under natural conditions. Similarly, the lipophilic substance 40 is also incompatible with the liquid contained in the cleaning chamber 12 . The role of the lipophilic substance 40 is to separate the liquid, such as cleaning fluid, in the lysis chamber 11 and the cleaning chamber 12. Through the separation function of the lipophilic substance 40, a barrier is formed between the lysis chamber 11 and the cleaning chamber 12 to prevent a cracking chamber from being blocked. The liquid between 11 and cleaning chamber 12 flows freely and mixing occurs. The lipophilic substance 40 can also prevent the detection liquid in the lysis chamber 11 and the cleaning chamber 12 from volatilizing.

For example, the lipophilic substance 40 may be lipophilic materials such as paraffin and oil. In some embodiments of the present application, in order to enable the lipophilic substance 40 to maintain a certain shape when the biological sample processing device 100 is not in use to avoid its displacement during transportation, paraffin wax can be used as the lipophilic substance 40, using Paraffin wax is solid at room temperature, so it has a fixed form when not in use. When used, the paraffin wax is melted by heating. In the following description, the lipophilic substance 40 is paraffin as an example.

In some embodiments of the present application, the biological sample processing device 100 further includes a magnetic conductive member 50. The magnetic conductive member 50 is received in at least one of the lysis chamber 11, the cleaning chamber 12 and the communication channel 13. The magnetic conductive member 50 is used. Under the action of an external magnetic field, it enters the cracking chamber 11 and adsorbs the target substance in the sample to be processed.

The function of the magnetic conductive member 50 is to adsorb the target substance and transport the target substance under the action of the external magnetic field, that is, to transfer the target substance through the lipophilic substance 40 between different sample processing chambers, so as to transport the adsorbed target substance to different sample processing chambers. cavities, for different treatments. The number of magnetic conductive members 50 can be set to multiple, and multiple magnetic conductive members 50 can be used to adsorb the target substance at the same time to improve the adsorption and transfer efficiency of the target substance.

Illustratively, in some embodiments of the present application, the magnetic conductive member 50 may be superparamagnetic magnetic particles.

In some embodiments of the present application, the external magnetic field can be a permanent magnet or an electromagnet. The external magnetic field is used to attract the plurality of magnetic conductive members 50 and the external magnetic field is moved along the structure of the sample processing member 10 to attract the plurality of magnetic conductive members 50 . Move into the sample to be processed in the cracking chamber 11, wait for the multiple magnetic conductive members 50 to adsorb the target substance, and then move the external magnetic field along the sample processing member 10, so that the target substance passes through the multiple sample processing chambers in sequence.

In some embodiments of the present application, the lipophilic substance 40 is solid at the first temperature and is at least partially accommodated in the communication channel 13 , and the magnetic conductive member 50 is sealed in the communication channel 13 through the lipophilic substance 40 .

The lipophilic substance 40 is solid at the first temperature. A possible implementation is that during the storage and transportation process after the biological sample processing device 100 is produced, the ambient temperature is maintained around the first temperature. At this time, the lipophilic substance 40 In solid state. In other words, the lipophilic substance 40 can be configured to remain solid at normal temperature. The specific value of the first temperature is related to the type of the lipophilic substance 40 . Therefore, the type of the lipophilic substance 40 can be determined according to the use environment of the biological sample processing device 100 .

For example, the melting point of the lipophilic substance 40 can be selected to be 20°C to 40°C higher than the first temperature to avoid the lipophilic substance 40 from melting at normal temperature (10°C to 40°C).

Since the role of the magnetic conductive member 50 is to adsorb the target substance and move under the action of an external magnetic field, the magnetic conductive member 50 cannot enter the sample processing chamber prematurely and come into contact with the detection liquid to avoid damaging the magnetic conductive member 50 or the sample to be processed. Make an impact. For example, if the magnetic conductive member 50 enters the lysis chamber prematurely and comes into contact with the detection liquid, during the lysis process, the magnetic conductive member 50 is likely to collide and rub with the lysis particles under the action of functional instruments, thereby causing damage to the magnetic conductive member. 50% surface damage, affecting the adsorption effect of subsequent target substances.

Based on this, in some embodiments of the present application, the magnetic conductive member 50 can be set to be sealed in the communication channel 13 through the lipophilic substance 40 . In this way, when the biological sample processing device 100 is not in use, the magnetic conductive member 50 can be fixed to prevent the magnetic conductive member 50 from moving in the sample processing member 10 before the biological sample processing device 100 is used.

In some embodiments of the present application, the lipophilic substance 40 may melt at a second temperature greater than the first temperature to flow into the first region 102 and float above the sample to be processed contained in the lysis chamber 11 .

The second temperature is a temperature higher than the melting point of the lipophilic substance 40. During the use of the biological sample processing device 100, an external functional instrument (such as a heating device) can be used to heat the lipophilic substance 40, so that the lipophilic substance 40 Melts and flows into the cracking chamber 11. At this time, the liquid lipophilic substance 40 floats on the detection liquid (sample to be processed) in the cracking chamber 11 (since the density of paraffin is lower than that of the detection liquid and it is incompatible with the detection liquid, Therefore, the paraffin can float above the detection liquid) and be located above the sample to be processed, thereby isolating the detection liquid in the cracking chamber 11 and the detection liquid in the cleaning chamber 12 .

In some embodiments of the present application, the magnetic conductive member 50 located in the communication channel 13 can pass through the lipophilic substance 40 into the lysis chamber 11 and contact the sample to be processed under the action of an external magnetic field.

The lipophilic substance 40 enters the lysis chamber 11 and comes into contact with the sample to be processed. A possible implementation is to use an external magnetic field to move the magnetic conductive member 50 after the work of functional instruments such as the heating device and ultrasonic device is completed (or almost before the end).

For example, taking the cracking chamber 11 as a cracking chamber, after the heating device completes heating and melting the lipophilic substance 40, and the ultrasonic device processes the sample to be processed in the cracking chamber, the sample located in the cracking chamber can be moved through an external magnetic field. The magnetic conductive member 50 in the communication channel 13 moves into the cracking chamber to adsorb the released target substance.

In some embodiments of the present application, the communication channel 13 includes a first port 131 adjacent to the lysis chamber 11 and a second port 132 adjacent to the cleaning chamber 12; the magnetic conductive member 50 is fixed on the first port 131 through the lipophilic substance 40. One port 131 or a second port 132.

The first port 131 is adjacent to the cracking chamber 11. A possible implementation is that the first port 131 is located at an end of the communication channel 13 close to the cracking chamber 11; the second port 132 is adjacent to the cleaning chamber 12. A possible implementation is, The second port 132 is located at one end of the communication channel 13 close to the cleaning chamber 12 . The plane where the first port 131 is located and the plane where the second port 132 is located may be parallel to each other or may be intersecting.

For example, the communication channel 13 may be straight-cylindrical. In this case, the first port 131 and the second port 132 are located at both ends of the communication channel 13 respectively, and the plane where the first port 131 is located and the plane where the second port 132 is located are mutually opposite to each other. parallel.

The magnetic conductive member 50 is fixed to the first port 131 or the second port 132 through the lipophilic substance 40. A possible implementation is that the magnetic conductive member 50 is disposed in the communication channel 13 close to the lysis chamber 11 or the cleaning chamber 12, so that the lipophilic material 40 can After melting, the substance 40 quickly enters the cracking chamber 11 or the cleaning chamber 12 and floats above the detection liquid to continue to play a separation role. At the same time, the magnetic conductive member 50 is located at one end of the communication channel 13 and can enter the cracking chamber 11 more quickly. Or cleaning chamber 12.

In some embodiments of the present application, the magnetic conductive member 50 includes magnetic particles (magnetic particles) or magnetic conductive balls. Magnetic particles refer to extremely small particles or particle aggregates with magnetic properties. They are usually composed of magnetic materials such as iron, nickel, cobalt, etc. The size of magnetic particles generally ranges from nanometers to micrometers. Magnetic particles have special magnetic behavior and can exhibit magnetization behavior under the action of an external magnetic field. Specifically, when a magnetic particle is exposed to an external magnetic field, its internal magnetic moment will be affected by the magnetic field, causing the particle itself to exhibit magnetism. This magnetization behavior can be permanent (hard magnetic) or reversible (soft magnetic), depending on the properties of the magnetic material. In this embodiment, the magnetic particles are mainly used for biological separation in biomedicine.

It should be noted that the application and research of magnetic particles also need to consider their impact on the environment and health, especially at the nanoscale. Therefore, relevant safety measures and regulations need to be observed when using and handling magnetic particles.

In some embodiments of the present application, the lysis chamber 11 includes a chamber bottom wall 111 and a plurality of chamber sidewalls 112 formed by bending and extending from the chamber bottom wall 111 . The chamber sidewalls 112 include spaced apart from the partition plate 20 . The first area side wall 1121 and the second area side wall 1122 are provided, the first area side wall 1121 is adjacent to the communication channel 13, and the distance between the partition 20 and the first area side wall 1121 is smaller than the distance between the partition 20 and the second area side wall 1121. The spacing between area sidewalls 1122.

The first area side wall 1121 is adjacent to the communication channel 13. A possible implementation is that the first area side wall 1121 is the side wall closest to the communication channel 13. At the same time, since the second area side wall 1122 is different from the first area side wall 1121 are relatively spaced apart, then the second area side wall 1122 is the side wall farthest from the communication channel 13 .

Based on this, the distance between the partition 20 and the first area side wall 1121 is smaller than the distance between the partition 20 and the second area side wall 1122 . A possible implementation is that the partition is arranged closer to the communication channel 13 . In this way, the volume of the first region 102 is smaller than the volume of the second region 103, thereby reducing the amount of lipophilic substance 40 and allowing the second region 103 to have a larger accommodation space.

In some embodiments of the present application, the sample processing component 10 further includes a communication channel 13 and an opening 14 of the lysis chamber 11 and the cleaning chamber 12 , and the sample adding component 30 further includes a cover 33 covering the opening 14 .

It should be noted that the above description of this embodiment is based on the description that the sample processing component 10 only includes the lysis chamber 11 and the cleaning chamber 12. In the case where the sample processing component 10 also includes a third sample processing chamber and a fourth sample processing chamber , the opening 14 can also communicate with the third sample processing chamber and the fourth sample processing chamber.

Based on this, the cover 33 covers the opening 14 by welding, so that the biological sample processing device 100 forms a sealed space. Before the cover 33 does not cover the opening 14, each sample processing chamber and each connecting channel 13 can be cleaned and disinfected through the opening 14, and the detection liquid is pre-stored in each sample processing chamber, the magnetic conductive member 50 is pre-stored in the connecting channel 13, and the magnetic conductive parts 50 are pre-stored in each connecting channel 13. The sample processing chamber and/or the communication channel 13 is pre-stored with a solid lipophilic substance 40 .

After the cover plate 33 covers the opening 14, the cover plate 33 and the partition plate 20 can be spaced apart, or the cover plate 33 and the partition plate 20 can be in contact. The partition plate 20 is vertically connected to the cover plate 33, which can be welded. , the snap connection can also be formed in one piece. This choice depends on the consideration of processing technology and cost, and does not affect the actual innovation point of this application. In these embodiments of the present application, in order to facilitate processing and molding, the contact between the cover plate 33 and the partition plate 20 can be selected. By arranging the contact between the cover plate 33 and the partition plate 20, the design of the mold can be simplified, thereby making molding more convenient.

At the same time, it should be noted that in the embodiment in which the cover plate 33 and the partition plate 20 are spaced apart, that is, the cover plate 33 and the partition plate 20 are not connected, there will be some lipophilic substances 40 passing between the cover plate 33 and the partition plate 20 The gap between them enters the second region 103. Since the lipophilic substance 40 floats above the detection liquid in both the first region 102 and the second region 103, it will not have any influence.

In some embodiments of the present application, the sample processing component 10 also includes the communication channel 13 and the opening 14 of the lysis chamber 11 and the cleaning chamber 12. The sample adding component 30 also includes a cover 33 covering the opening 14; the partition 20 is connected to the opening 14. The cover plate 33 is connected.

Based on this, the partition plate 20 is directly connected to the cover plate 33, which reduces the difficulty of forming the partition plate 20. At the same time, when the cover plate 33 does not cover the opening 14, it is also convenient to add detection liquid to each sample processing chamber and to the lysis chamber. Add lysis particles to the chamber, add lyophilized reaction components to the amplification chamber, etc.

The connection method between the partition plate 20 and the cover plate 33 can be bonding, riveting, integral molding, etc.

In some embodiments of the present application, the lysis chamber 11 includes a chamber bottom wall 111 and a plurality of chamber side walls 112 formed by bending and extending from the chamber bottom wall 111. The partition 20 is in contact with the chamber side in its width direction. Wall 112 interference fit.

Based on this, in some embodiments of the present application, the width of the partition 20 can be set to be slightly larger than the distance between the chamber side walls 112 on both sides of the partition 20 in the width direction. In this way, after the cover 33 covers the opening 14, the partition 20 Both sides of the width direction of 20 are closely matched with the chamber side wall 112 to prevent the lipophilic substance 40 from passing through the gap between the partition 20 and the chamber side wall 112 from the first area into the second area during subsequent use. regional situation.

In some embodiments of the present application, the lysis chamber 11 includes a chamber bottom wall 111, a plurality of chamber side walls 112 formed by bending and extending from the chamber bottom wall 111, and a plurality of chamber side walls 112 formed by extending inwardly from the chamber side wall 112. Guide rail (not shown in the figure), the partition 20 is clamped on the guide rail.

By arranging guide rails on the side wall 112 of the chamber, the guide rails are used to provide guidance for the insertion of the partition plate 20, which facilitates subsequent covering of the opening 14 with the cover plate 33.

In some embodiments of the present application, the number of guide rails may be one. One guide rail is provided on the chamber side wall 112 on one side of the sample processing chamber. During the subsequent process of covering the opening 14 with the cover plate 33, a single-sided rail is used. Provide guidance for the partition 20.

At the same time, in other embodiments of the present application, the number of guide rails can be set to two, and the two guide rails are respectively provided on the two opposite chamber side walls 112 of the sample processing chamber, and the two guide rails are used to simultaneously The partition plate 20 can be inserted for guidance, and at the same time, the partition plate 20 can also be clamped by two guide rails. Therefore, compared with the previous embodiments of the present application, the width of the partition plate 20 can be set to be equal to or smaller than the chamber side walls on both sides of the width direction. The spacing between 112mm makes installation more convenient.

In some embodiments of the present application, the cracking chamber 11 is pre-stored with cracking aids for crushing the sample to be processed, and/or the cleaning chamber 12 is pre-stored with cleaning reagents for cleaning the sample to be processed, Cleaning is a cleaning process.

The cracking aids are the cracking particles provided in the aforementioned embodiments, which can be polystyrene particles, borosilicate glass particles, quartz glass particles, etc. The cracking chamber 11 is a cracking chamber, and the cleaning chamber 12 is a cleaning chamber.

In these embodiments of the present application, a partition 20 is provided in the cracking chamber 11 as a cracking chamber, and the cracking chamber 11 is divided into a first area 102 and a third area in which the bottom areas are connected to each other and the areas corresponding to the partition 20 are isolated from each other. Area 2103. While the input amount of the lipophilic substance 40 into the lysis chamber 11 can be reduced, the accommodation space of the second region 103 can also be expanded to accommodate the sample adding member 30 .

In some embodiments of the present application, a preset distance is spaced between the bottom of the partition 20 and the bottom of the cracking chamber 11 so that the bottom areas of the first region 102 and the second region 103 are connected to each other.

Based on this, by providing a gap between the bottom of the partition 20 and the cracking chamber 11 , that is, the bottom ends of the first region 102 and the second region 103 are completely connected, which does not affect the amount of sample to be processed that can be injected into the cracking chamber 11 , and It is beneficial for the magnetic conductive member 50 to move between the first area 102 and the second area 103 .

It should be noted that, on the premise of ensuring that the liquid level of the detection liquid contained in the cracking chamber 11 is higher than the bottom end of the partition 20 , the preset distance can be set according to the actual situation.

In a second aspect, embodiments of the present application provide a biological detection system, including the biological sample processing device 100 of any of the foregoing embodiments and a magnet, and/or a heating device, and/or an ultrasonic device. Among them, the magnet can be in contact with the outer chamber side wall of the sample processing component 10 and be used to provide an external magnetic field; the heating device can be in contact with the outer chamber side wall of the communication channel 13 and/or the cleaning chamber 12 and be used for heating; the ultrasonic device It is in contact with the outer chamber side wall of the cracking chamber 11 and is used to emit ultrasonic waves to the cracking chamber 11 and/or the communication channel 13 .

Figure 7 is a schematic diagram of a biological sample processing device provided by some embodiments of the present application. Figure 6 is a schematic diagram of the prior art. The sample 400 to be processed in the figure can be pre-packaged in the biological sample processing device before the biological sample processing device 100 is manufactured. In the device, the user can also join during the detection process. But no matter which way, in order to accurately determine the detection results, the amount of the sample to be processed 400 is maintained within a range, and the cracking chamber 11 should not be set too long, so the cross-sectional area of the cracking chamber 11 is relatively fixed. In this case, since the magnetic particles need to be wrapped with a sufficient amount of paraffin 300 to prevent the loss of magnetic beads during the movement of the magnetic particles, as shown in Figure 6, the paraffin 300 in liquid form needs to cover the entire cross-section of the cracking chamber 11, resulting in Paraffin 300 uses a large amount, which does not help save materials and melt quickly. Using the solution shown in Figure 7 in this embodiment, without increasing additional costs, by setting the partition 20, the liquid paraffin 300 that later enters the cracking chamber 11 can be separated into a smaller area. , and on the necessary path during the movement of magnetic particles, while reducing the amount of paraffin 300, it can still ensure that the magnetic particles are wrapped in sufficient paraffin during the movement. This ensures the detection efficiency and accuracy of the detection results, and at the same time reduces the overall size of the biological sample processing device 100, further ensuring the convenience of transportation, storage, and user use.

The above are only specific implementation modes of the embodiments of the present application, but the protection scope of the embodiments of the present application is not limited thereto. Any person familiar with the technical field can easily think of various methods within the technical scope disclosed in the embodiments of the present application. Equivalent modifications or substitutions shall be included within the protection scope of the embodiments of this application. Therefore, the protection scope of the embodiments of the present application should be subject to the protection scope of the claims.

Claims (15)

1. A biological sample processing device, characterized in that it includes: sample processing components; It includes a lysis chamber for receiving a sample to be processed and lysing the sample to be processed; It includes a cleaning chamber arranged side by side with the lysis chamber and used for cleaning samples to be processed; And a communication channel connecting the top of the cracking chamber and the cleaning chamber; The lysis chamber is used to accommodate an aqueous solution containing a sample to be processed; A partition is provided in the cracking chamber. The partition divides the cracking chamber into a first area and a second area in which bottom areas are connected to each other and areas corresponding to the partition are isolated from each other. The first area is located in the between the connecting channel and the second region; When the aqueous solution containing the sample to be processed is accommodated in the lysis chamber, one end of the partition extends below the surface of the aqueous solution; Between the first region and the end of the communication channel, there is also a lipophilic substance that can change its shape with temperature changes; the lipophilic substance isolates the cracking chamber from the cracking chamber in a solid form at normal temperature. The liquid in the cleaning chamber flows; the lipophilic substance covers the aqueous solution in liquid form, and the lipophilic substance and the aqueous solution are immiscible with each other. 2. The biological sample processing device according to claim 1, characterized in that the biological sample processing device further includes a magnetic conductive member, and the magnetic conductive member is accommodated in the lysis chamber, the cleaning chamber and the communication chamber. In at least one of the channels, the magnetic conductive member is used to enter the lysis chamber and adsorb the target substance in the sample to be processed under the action of an external magnetic field. 3. The biological sample processing device according to claim 2, characterized in that the lipophilic substance is solid at the first temperature and is at least partially contained in the communication channel, and the magnetic conductive member is sealed in of the lipophilic substances. 4. The biological sample processing device according to claim 3, wherein the lipophilic substance can melt at a second temperature to flow into the first region and float on the material to be processed contained in the lysis chamber. Above the sample, the second temperature is greater than the first temperature. 5. The biological sample processing device according to claim 4, characterized in that the magnetic conductive member located in the communication channel can pass through the lipophilic substance and enter the lysis chamber under the action of an external magnetic field. And contact with the sample to be processed to capture the target substance, and pass through the lipophilic substance to the cleaning chamber. 6. The biological sample processing device according to claim 5, wherein the communication channel includes a first port adjacent to the lysis chamber and a second port adjacent to the cleaning chamber; The magnetic conductive member is fixed on the first port or the second port through the lipophilic substance. 7. The biological sample processing device according to claim 1, wherein the lysis chamber includes a chamber bottom wall and a plurality of chamber side walls formed by bending and extending from the chamber bottom wall. The chamber side wall includes a first area side wall and a second area side wall that are spaced apart from the partition plate, the first area side wall is adjacent to the communication channel, and the partition plate is connected to the first area side wall. The distance between side walls is smaller than the distance between the partition and the side walls of the second region. 8. The biological sample processing device according to claim 1, characterized in that, the biological sample processing device further comprises a sample adding member, the sample adding member is at least partially inserted into the second region, the sample adding member has A receiving cavity for receiving the sample to be processed and a filter outlet connecting the receiving cavity and the second area. 9. The biological sample processing device according to claim 8, characterized in that the communication channel, the lysis chamber and the cleaning chamber have openings in the same direction, and the sample adding member further includes a cover plate; The cover plate and the partition plate are spaced apart; Alternatively, the cover plate is in contact with the partition plate. 10. The biological sample processing device according to claim 8, wherein the sample processing component further includes an opening connecting the communication channel with the lysis chamber and the cleaning chamber, and the sample adding component further includes A cover plate covers the opening, and the partition plate is connected to the cover plate. 11. The biological sample processing device according to claim 7, wherein the lysis chamber includes a chamber bottom wall and a plurality of chamber side walls bent and extended from the chamber bottom wall, and the partitions The plate has an interference fit with the chamber side wall in its width direction. 12. The biological sample processing device according to claim 7, wherein the lysis chamber includes a chamber bottom wall, a plurality of chamber side walls formed by bending and extending from the chamber bottom wall, and a plurality of chamber side walls extending from the chamber bottom wall. The side wall of the chamber extends inward to form a guide rail, and the partition plate is clamped on the guide rail. 13. The biological sample processing device according to claim 1, wherein a cracking aid for crushing the sample to be processed is pre-stored in the cracking chamber. 14. The biological sample processing device according to claim 1, wherein a preset distance is spaced between the bottom of the partition and the bottom of the lysis chamber, so that the distance between the first region and the second region The bottom areas are interconnected. 15. A biological detection system, characterized by comprising: The biological sample processing device according to any one of claims 1-14; and A magnet, which is in contact with the side wall of the outer chamber of the sample processing component and used to provide an external magnetic field; A heating device is in contact with the communication channel or the outer chamber side wall of the cleaning chamber and is used for heating; An ultrasonic device is in contact with the side wall of the outer chamber of the lysis chamber and used to emit ultrasonic waves to the lysis chamber or the communication channel.