CN116519968A - Integrated multi-reagent sample mixing and adding device - Google Patents
Integrated multi-reagent sample mixing and adding device Download PDFInfo
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- CN116519968A CN116519968A CN202310751176.XA CN202310751176A CN116519968A CN 116519968 A CN116519968 A CN 116519968A CN 202310751176 A CN202310751176 A CN 202310751176A CN 116519968 A CN116519968 A CN 116519968A
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- 238000002156 mixing Methods 0.000 title claims abstract description 171
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 87
- 238000002347 injection Methods 0.000 claims abstract description 40
- 239000007924 injection Substances 0.000 claims abstract description 40
- 210000004369 blood Anatomy 0.000 claims description 34
- 239000008280 blood Substances 0.000 claims description 34
- 239000012528 membrane Substances 0.000 claims description 34
- 238000002955 isolation Methods 0.000 claims description 22
- 239000003219 hemolytic agent Substances 0.000 claims description 12
- 239000011324 bead Substances 0.000 claims description 11
- 239000003146 anticoagulant agent Substances 0.000 claims description 10
- 238000011068 loading method Methods 0.000 claims description 10
- 229940127219 anticoagulant drug Drugs 0.000 claims description 9
- 238000007789 sealing Methods 0.000 claims description 6
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims description 4
- 230000004888 barrier function Effects 0.000 claims description 3
- 238000013461 design Methods 0.000 abstract description 6
- 238000001514 detection method Methods 0.000 abstract description 6
- 239000000463 material Substances 0.000 abstract description 6
- 238000000034 method Methods 0.000 description 18
- 230000008569 process Effects 0.000 description 14
- 230000010100 anticoagulation Effects 0.000 description 9
- 230000009286 beneficial effect Effects 0.000 description 8
- 230000009471 action Effects 0.000 description 6
- 239000012943 hotmelt Substances 0.000 description 5
- 238000005070 sampling Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 238000007731 hot pressing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 206010018910 Haemolysis Diseases 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000010339 medical test Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses an integrated multi-reagent sample mixing and adding device in the technical field of reagent sample mixing, which comprises a first reagent body, a second reagent body and a collecting body; the second reagent body and the acquisition body are respectively arranged at two sides of the first reagent body; the collecting body comprises a collecting block, a siphon pipe is arranged in the collecting block, the collecting block is fixedly connected with a first piercing block, and the first piercing block is arranged outside the siphon pipe in a surrounding manner; the first reagent body comprises a mixing tube, a first isolating film is arranged in the mixing tube, the mixing tube is fixedly connected with a plurality of puncture blocks, and a second isolating film is arranged in one side of the mixing tube; the second reagent body comprises an injection pipe, a third isolating film is arranged in one side of the injection pipe, and a second puncture block is arranged on the inner wall of one side of the injection pipe, which is far away from the puncture block. The invention has simple structure, can realize that the operations of sucking samples, mixing reagents and adding samples are all completed in one device through the design of the first reagent body, the second reagent body and the acquisition body, and has high efficiency, convenience, low cost and reduced consumable materials required by detection.
Description
Technical Field
The invention belongs to the technical field of reagent sample mixing, and particularly relates to an integrated multi-reagent sample mixing and adding device.
Background
In many medical tests, a blood sample is subjected to a pretreatment process in which individual reagents are added to the blood, which are not usually mixed together in advance, and which require time intervals. The reagent is sequentially added through the pipettor or the dropper, and finally the reagent is sucked out and loaded into the detector through the pipettor or the dropper, so that the whole process is complex in operation and more in consumable materials are needed.
For example, for leukocyte analysis, the process flow to be performed is as follows (in the case of quantitative droppers only, pipettes are equivalent): the first step: opening the anticoagulation tube, using the quantitative dropper 1 to drop the blood into the anticoagulation tube, covering the anticoagulation tube for shaking, and fully fusing the anticoagulation agent and the blood; and a second step of: opening an anticoagulation tube, using a quantitative dropper 2 to inhale the hemolytic agent from the hemolytic agent, adding the hemolytic agent into the anticoagulation tube, covering the anticoagulation tube for shaking, fusing the hemolytic agent with the previous sample, waiting for a period of time, and waiting for the completion of hemolysis; and a third step of: opening an anticoagulation tube, sucking out a sample from the anticoagulation tube by using a quantitative dropper 3, adding the sample into a detector for inspection, covering the anticoagulation tube to avoid pollution, and throwing the sample into a garbage can;
according to the steps, in order to avoid cross contamination of the sample and the reagent and complete detection, 3 quantitative droppers are required to be consumed, an anticoagulant tube and a hemolytic agent are required to be prepared respectively, the anticoagulant tube is required to be opened and closed repeatedly in the operation process, and the operation is complicated.
Therefore, it is necessary to provide an integrated multi-reagent sample mixing and adding device, so that the operations of sample sucking, reagent mixing and sample adding are completed in one device, and the device is efficient, convenient, low in cost and capable of reducing consumable materials required for detection.
Disclosure of Invention
In order to solve the problem that the operation is complicated, the invention aims to provide an integrated multi-reagent sample mixing and adding device, and through the design of a first reagent body, a second reagent body and a collecting body, the operations of sample suction, reagent mixing and sample adding can be completed in one device, so that the device is efficient, convenient and low in cost, and consumable materials required by detection are reduced.
In order to achieve the above object, the technical scheme of the present invention is as follows: an integrated multi-reagent sample mixing and adding device comprises a first reagent body, a second reagent body and a collecting body; the second reagent body and the acquisition body are respectively arranged at two sides of the first reagent body;
the collection body includes the collection piece, is equipped with the siphon that is used for absorbing blood in the collection piece, and outside the collection piece was passed and extended to the collection piece to siphon one end, the collection piece was close to first reagent body one side fixedly connected with first puncture piece, and first puncture piece ring is established in the siphon outside, and collection piece is kept away from first puncture piece one side and can be dismantled and be connected with the closing cap that is used for sealing the siphon.
The first reagent body comprises a mixing pipe, an anticoagulant is arranged in the mixing pipe, the diameter of the mixing pipe is larger than that of the collecting block, the outer wall of the collecting block can be attached to the inner wall of the mixing pipe, a first isolating membrane is arranged in the mixing pipe, the first isolating membrane is positioned on one side, close to the siphon, of the mixing pipe, a plurality of puncture blocks are fixedly connected to one side, far away from the first isolating membrane, of the mixing pipe, and a second isolating membrane is arranged in one side, close to the puncture blocks, of the mixing pipe; and the first barrier film is capable of being pierced by the first piercing block.
The second reagent body comprises an injection pipe, a hemolytic agent is arranged in the injection pipe, the diameter of the injection pipe is larger than that of the mixing pipe, the outer wall of the mixing pipe can be attached to the inner wall of the injection pipe, a third isolating membrane is arranged in one side, close to the mixing pipe, of the injection pipe and can be pierced by a piercing block, a second piercing block is arranged on the inner wall, far away from the piercing block, of the injection pipe, and the second piercing block can pierce the third isolating membrane and the second isolating membrane.
The basic scheme is as follows: sucking a certain amount of blood by using the siphon tube, then placing the collecting body close to the mixing tube and placing the siphon tube and the first puncture block close to the first isolation film, wherein the first isolation film is punctured, so that the mixing tube and the siphon tube are communicated, and the blood in the siphon tube flows into the mixing tube, so that the blood is mixed with the anticoagulant in the mixing tube, and the mixing tube is shaken by hands during mixing to promote the blood and the anticoagulant to be fully mixed;
the side of the mixing tube far away from the collecting body is close to the injection tube, so that a puncture block on the mixing tube gradually punctures the third isolating membrane, and meanwhile, because the mixing tube and the injection tube are gradually close to each other, the second puncture block is also synchronously close to the third isolating membrane and the second isolating membrane, so that the mixing tube and the injection tube are communicated, the hemolytic agent is mixed with blood, and meanwhile, the mixing tube is shaken by hands during mixing to promote the blood and the hemolytic agent to be fully mixed; when the sample is required to be taken out after mixing, the sealing cover is taken down, and the mixing tube is pressed by hand, so that the sample in the siphon tube can be promoted to be taken out.
The basic scheme has the beneficial effects that: compared with the prior art, the design of this technical scheme through the first reagent body, the second reagent body and gathering the body can realize that inhale appearance, mixed reagent, application of sample operation are all accomplished in a device, and high efficiency is convenient, with low costs, reduce the required consumptive material of detection.
Further, the outer wall of the collecting block is provided with a first external thread strip, the outer part of the mixing tube, which is close to the puncture block, is provided with a second external thread strip, the inner wall of the mixing tube, which is close to the first isolating film, is provided with a first internal thread strip which is in threaded fit with the first external thread strip, and the inside of the injection tube is provided with a second internal thread strip which is in threaded fit with the second external thread strip.
The basic scheme has the beneficial effects that: through the design of the internal and external screw thread strips, the collection body, the mixing tube and the injection tube can be gradually close to and communicated with each other, and meanwhile, the internal and external screw thread strips can facilitate the fixation of the collection body, the mixing tube and the injection tube, so that the device is convenient to shake by hands subsequently, and the sample and the blood are fully mixed.
Further, a plurality of mixing beads are arranged in the mixing tube, and the diameter of the mixing beads is larger than that of the siphon tube.
The basic scheme has the beneficial effects that: the mixing beads in the mixing tube can flow in the mixing tube by shaking the mixing tube, so that the mixing efficiency of the blood sample plate and the reagent can be increased under the drive of the mixing beads, and further, more efficient mixing is realized.
Further, the first puncture block and the second puncture block are arranged in a staggered mode.
The basic scheme has the beneficial effects that: through first puncture piece and second puncture piece dislocation set, the conflict that the mutual top of first puncture piece and second puncture piece reached can be reduced to can increase the first puncture piece and the second puncture piece extend to the degree of depth in the hybrid tube, and then increase the width that the barrier film was punctured, thereby reducible flow and the mixing of reagent.
Further, the first, second and third isolation films are all tinfoil.
The basic scheme has the beneficial effects that: the foil paper has the characteristics of good tightness and easy puncture, so that the first isolating film, the second isolating film and the third isolating film can respectively seal the reagent in the mixing tube and the injection tube on one hand, and on the other hand, the first isolating film, the second isolating film and the third isolating film can be easily punctured by the puncturing block and the puncturing block, so that the reagent and the blood sample are mixed.
Further, the interior of the mixing tube is in a negative pressure state.
The basic scheme has the beneficial effects that: when the first puncture block punctures the first isolating membrane, a certain suction force is generated in the mixing tube under the action of negative pressure, so that the blood sample in the siphon tube is guided into the mixing tube by the suction force of the negative pressure, the mixing speed of the blood sample and the reagent can be increased, and the mixing efficiency of the reagent and the blood sample can be improved.
Further, support rods are symmetrically arranged in the mixing tube, a guide ring is fixedly connected between the two support rods, an annular groove is arranged in the guide ring, a rotating ring is arranged in the guide ring, an annular sliding block is arranged outside the rotating ring, the sliding block is positioned in the annular groove, the guide ring and the rotating ring are in rotating fit with each other through the annular groove, and one end, far away from the first isolation film, of the rotating ring is fixedly connected with an inclined mixing rod;
the clamping grooves are symmetrically and fixedly connected to the inner wall of the rotating ring, a plurality of clamping blocks corresponding to the clamping grooves are fixedly connected to one end, away from the collecting body, of the siphon pipe, the clamping blocks can extend into the clamping grooves, and the clamping blocks and the clamping grooves can lock the positions of the siphon pipe and the rotating ring.
The basic scheme has the beneficial effects that: the clamping block on the siphon pipe is clamped in the clamping groove, then when the first external thread strip is in contact with the first internal thread strip, the collecting body is rotated, the first external thread strip and the first internal thread strip are in threaded fit, when the collecting body is rotated, the siphon pipe is rotated along with the first external thread strip, the rotating ring is driven by the siphon pipe to rotate, and when the rotating ring is rotated, the mixing rod is rotated along with the rotating ring, so that the mixing pipe and the collecting body can be locked, and meanwhile, the blood sample and the reagent are mixed in a rotating mode under stirring of the remixing rod, the sample and the reagent are fully mixed, and the mixing efficiency of the sample and the reagent is improved.
Further, the mixing rod and the second piercing block are arranged in a staggered manner.
The basic scheme has the beneficial effects that: when the second puncture block punctures the isolating membrane, the mixing rod can not influence the puncturing operation of the second puncture block, so that the stability of the device is improved.
Drawings
FIG. 1 is a side view of an integrated multi-reagent sample mixing and loading device according to an embodiment of the present invention.
FIG. 2 is a side view of a mixing rod of an integrated multi-reagent sample mixing and loading device according to an embodiment of the present invention.
FIG. 3 is a top view of a rotating ring of an integrated multi-reagent sample mixing and loading device according to an embodiment of the present invention.
Detailed Description
The following is a further detailed description of the embodiments:
reference numerals in the drawings of the specification include: the device comprises a collection body 1, a collection block 101, a siphon 102, a first external thread 103, a first puncture block 104, a closing cap 105, a clamping block 106, a first reagent body 2, a mixing tube 201, a first isolating membrane 202, a first internal thread 203, a mixing bead 204, a second isolating membrane 205, a second external thread 206, a puncture block 207, a support rod 208, an annular groove 209, a guide ring 210, a mixing rod 211, a rotating ring 212, a clamping groove 213, a second reagent body 3, an injection tube 301, a third isolating membrane 302, a second internal thread 303 and a second puncture block 304.
Example 1, substantially as shown in figure 1: an integrated multi-reagent sample mixing and adding device comprises a first reagent body 2, a second reagent body 3 and a collecting body 1; the second reagent body 3 and the collection body 1 are respectively arranged at two sides of the first reagent body 2;
the collection body 1 comprises a collection block 101, a siphon 102 for sucking blood is fixedly adhered in the collection block 101, one end of the siphon 102 penetrates through the collection block 101 and extends out of the collection block 101, a first puncture block 104 is welded on the left side of the collection block 101 in a hot-melt mode, the first puncture block 104 is arranged outside the siphon 102 in a surrounding mode, and a sealing cover 105 for sealing the siphon 102 is detachably connected on the right side of the collection block 101.
The first reagent body 2 comprises a mixing tube 201, an anticoagulant is arranged in the mixing tube 201, the diameter of the mixing tube 201 is larger than that of the collecting block 101, the outer wall of the collecting block 101 can be attached to the inner wall of the mixing tube 201, a first isolating membrane 202 is arranged in the mixing tube 201, the first isolating membrane 202 is positioned on the right side of the mixing tube 201, a plurality of puncture blocks 207 are fixedly connected to the left side of the mixing tube 201, and a second isolating membrane 205 is arranged in the left side of the mixing tube 201; and the first separation membrane 202 is capable of being pierced by the first piercing block 104; the first and second separation films 202 and 205 are fixedly connected in the mixing pipe 201 by hot pressing.
The second reagent body 3 comprises an injection pipe 301, a hemolytic agent is arranged in the injection pipe 301, the diameter of the injection pipe 301 is larger than that of the mixing pipe 201, the outer wall of the mixing pipe 201 can be attached to the inner wall of the injection pipe 301, a third isolating membrane 302 is arranged in the right portion of the injection pipe 301, the third isolating membrane 302 can be pierced by the piercing block 207, a second piercing block 304 is arranged on the left inner wall of the injection pipe 301, and the second piercing block 304 can pierce the third isolating membrane 302 and the second isolating membrane 205. The third separation film 302 is fixedly connected to the inside of the injection pipe 301 by hot pressing.
The specific implementation process is as follows: when mixing a blood sample and a reagent, firstly, a certain amount of blood is sucked by the siphon tube 102, then the collector 1 is close to the mixing tube 201, the siphon tube 102 and the first puncture block 104 are close to the first isolation film 202, then the first isolation film 202 is punctured under the puncture action of the first puncture block 104, so that the mixing tube 201 and the siphon tube 102 are communicated, the blood in the siphon tube 102 flows into the mixing tube 201, the anticoagulant in the mixing tube 201 is mixed, and the mixing tube 201 is shaken by hands during mixing to promote the blood and the anticoagulant to be fully mixed;
then, the side of the mixing tube 201 away from the collection body 1 is close to the injection tube 301, so that the puncture block 207 on the mixing tube 201 gradually punctures the third isolation membrane 302, and meanwhile, because the mixing tube 201 and the injection tube 301 are gradually close to each other, the second puncture block 304 is also synchronously close to the third isolation membrane 302 and the second isolation membrane 205, so that the mixing tube 201 and the injection tube 301 are communicated, and thus the hemolytic agent is mixed with blood, and meanwhile, the mixing tube 201 is shaken by hand during mixing to promote the blood and the hemolytic agent to be fully mixed; when the sample is to be taken out after mixing, the closing cap 105 is removed, and the mixing tube 201 is pressed by hand, so that the sample in the siphon tube 102 can be taken out.
Compared with the prior art, the design of the first reagent body 2, the second reagent body 3 and the collection body 1 can realize that sample suction, reagent mixing and sample adding operations are finished in one device, and the method is efficient, convenient, low in cost and capable of reducing consumable materials required for detection.
Embodiment 2 is different from the above embodiment in that the outer wall of the collecting block 101 is provided with a first external thread strip 103, the outer portion of the mixing tube 201 near the puncture block 207 is provided with a second external thread strip 206, the inner wall of the mixing tube 201 near the first isolation film 202 is provided with a first internal thread strip 203 in threaded fit with the first external thread strip 103, and the injection tube 301 is internally provided with a second internal thread strip 303 in threaded fit with the second external thread strip 206.
The specific implementation process is as follows: when the sampling body 1, the mixing tube 201 and the injection tube 301 are utilized to mix the sample and the reagent, the sampling body 1, the mixing tube 201 and the injection tube 301 can be gradually close to and communicated with each other through the design of the internal and external screw thread strips, and meanwhile, the internal and external screw thread strips can facilitate the fixation of the sampling body 1, the mixing tube 201 and the injection tube 301, thereby facilitating the subsequent shaking of the device by hands so as to fully mix the sample and the blood.
Embodiment 3 differs from the above embodiment in that a plurality of mixing beads 204 are provided in the mixing tube 201, and the diameter of the mixing beads 204 is larger than the diameter of the siphon tube 102.
The specific implementation process is as follows: when mixing a blood sample and a reagent, the mixing beads 204 in the mixing tube 201 can flow in the mixing tube 201 by shaking the mixing tube 201, so that the mixing efficiency of the blood sample and the reagent can be increased under the driving of the mixing beads 204, and further, more efficient mixing is realized.
Embodiment 4 differs from the previous embodiments in that the first lancing block 104 and the second lancing block 304 are offset.
The specific implementation process is as follows: when the injection tube 301 approaches the mixing tube 201 and the second piercing block 304 pierces the isolation film, the second piercing block 304 will extend into the mixing tube 201, so that the collision of the first piercing block 104 and the second piercing block 304 with each other can be reduced by the dislocation arrangement of the first piercing block 104 and the second piercing block 304, and the depth of the first piercing block 104 and the second piercing block 304 extending into the mixing tube 201 can be increased, so as to increase the pierced width of the isolation film, and reduce the flow and mixing of the reagents.
Embodiment 5 is different from the above embodiment in that the first separator 202, the second separator 205, and the third separator 302 are all tinfoil.
The specific implementation process is as follows: the tinfoil paper has the characteristics of good tightness and easy puncture, so that the first isolation film 202, the second isolation film 205 and the third isolation film 302 can respectively seal the reagent in the mixing tube 201 and the injection tube 301 on one hand, and can puncture the first isolation film 202, the second isolation film 205 and the third isolation film 302 by the puncture block 207 and further mix the reagent with the blood sample on the other hand.
Example 6 is different from the above-described examples in that the inside of the mixing tube 201 is in a negative pressure state, and the mixing tube 201 is prepared in an internal negative pressure state at the time of factory preparation.
The specific implementation process is as follows: because the interior of the mixing tube 201 is under negative pressure, when the first puncture block 104 punctures the first isolation membrane 202, a certain suction force is generated in the mixing tube 201 under the action of the negative pressure, and thus the blood sample in the siphon tube 102 is guided into the mixing tube 201 by the suction force of the negative pressure, so that the mixing speed of the blood sample and the reagent can be increased, and the mixing efficiency of the reagent and the blood sample can be improved.
Embodiment 7 is different from the above embodiment in that, as shown in fig. 2-3, support rods 208 are symmetrically welded in the mixing tube 201 in a hot-melt manner, a guide ring 210 is welded between the two support rods 208, an annular groove 209 is formed in the guide ring 210, a rotating ring 212 is arranged in the guide ring 210, an annular sliding block is welded outside the rotating ring 212 in a hot-melt manner, the sliding block is positioned in the annular groove 209, the guide ring 210 and the rotating ring 212 are in a rotating fit with the sliding block through the annular groove 209, and an inclined mixing rod 211 is welded at one end of the rotating ring 212 far away from the first isolation film 202 in a hot-melt manner;
the inner wall of the rotating ring 212 is symmetrically provided with clamping grooves 213, one end, far away from the acquisition body 1, of the siphon 102 is welded with a plurality of clamping blocks 106 corresponding to the clamping grooves 213 in a hot-melt mode, the clamping blocks 106 can extend into the clamping grooves 213, and the clamping blocks 106 and the clamping grooves 213 can lock positions of the siphon 102 and the rotating ring 212.
The specific implementation process is as follows: when the collector 1 and the mixing tube 201 are communicated, the siphon tube 102 contacts the rotating ring 212, wherein the clamping block 106 on the siphon tube 102 is clamped in the clamping groove 213, then when the first external thread strip 103 contacts with the first internal thread strip 203, the collector 1 is rotated, the first external thread strip 103 and the first internal thread strip 203 are in threaded fit, when the collector 1 rotates, the siphon tube 102 also rotates, the rotating ring 212 is driven by the siphon tube 102 to rotate, and when the rotating ring 212 rotates, the mixing rod 211 rotates, so that the mixing tube 201 and the collector 1 are locked, and simultaneously the blood sample and the reagent are rotationally mixed under the stirring of the mixing rod 211, so that the full mixing of the sample and the reagent is realized, and the mixing efficiency of the sample and the reagent is improved.
Embodiment 8 differs from the above embodiment in that the mixing rod 211 is offset from the second piercing block 304.
The specific implementation process is as follows: because the mixing rod 211 and the second piercing block 304 are arranged in a staggered manner, when the second piercing block 304 pierces the isolation film, the mixing rod 211 will not affect the piercing operation of the second piercing block 304, so that the stability of the device is improved.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
The foregoing is merely an embodiment of the present invention, and a specific structure and characteristics of common knowledge in the art, which are well known in the scheme, are not described herein, so that a person of ordinary skill in the art knows all the prior art in the application day or before the priority date of the present invention, and can know all the prior art in the field, and have the capability of applying the conventional experimental means before the date, so that a person of ordinary skill in the art can complete and implement the present embodiment in combination with his own capability in the light of the present application, and some typical known structures or known methods should not be an obstacle for a person of ordinary skill in the art to implement the present application. It should be noted that modifications and improvements can be made by those skilled in the art without departing from the structure of the present invention, and these should also be considered as the scope of the present invention, which does not affect the effect of the implementation of the present invention and the utility of the patent. The protection scope of the present application shall be subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.
Claims (8)
1. An integrated multi-reagent sample mixing and adding device is characterized in that: the device comprises a first reagent body, a second reagent body and a collecting body; the second reagent body and the acquisition body are respectively arranged at two sides of the first reagent body;
the collecting body comprises a collecting block, a siphon pipe for sucking blood is arranged in the collecting block, one end of the siphon pipe penetrates through the collecting block and extends out of the collecting block, one side, close to the first reagent body, of the collecting block is fixedly connected with a first puncturing block, the first puncturing block is arranged outside the siphon pipe in a surrounding mode, and one side, far away from the first puncturing block, of the collecting block is detachably connected with a sealing cover for sealing the siphon pipe;
the first reagent body comprises a mixing pipe, an anticoagulant is arranged in the mixing pipe, the diameter of the mixing pipe is larger than that of the collecting block, the outer wall of the collecting block can be attached to the inner wall of the mixing pipe, a first isolating membrane is arranged in the mixing pipe, the first isolating membrane is positioned on one side, close to the siphon, of the mixing pipe, a plurality of puncture blocks are fixedly connected to one side, far away from the first isolating membrane, of the mixing pipe, and a second isolating membrane is arranged in one side, close to the puncture blocks, of the mixing pipe; and the first barrier film is capable of being pierced by the first piercing block;
the second reagent body comprises an injection pipe, a hemolytic agent is arranged in the injection pipe, the diameter of the injection pipe is larger than that of the mixing pipe, the outer wall of the mixing pipe can be attached to the inner wall of the injection pipe, a third isolating membrane is arranged in one side, close to the mixing pipe, of the injection pipe and can be pierced by a piercing block, a second piercing block is arranged on the inner wall, far away from the piercing block, of the injection pipe, and the second piercing block can pierce the third isolating membrane and the second isolating membrane.
2. The integrated multi-reagent sample mixing and loading device of claim 1, wherein: the outer wall of the collecting block is provided with a first external thread strip, the outer part of the mixing tube, which is close to the puncture block, is provided with a second external thread strip, the inner wall of the mixing tube, which is close to the first isolating film, is provided with a first internal thread strip which is in threaded fit with the first external thread strip, and the inside of the injection tube is provided with a second internal thread strip which is in threaded fit with the second external thread strip.
3. The integrated multi-reagent sample mixing and loading device of claim 2, wherein: a plurality of mixing beads are arranged in the mixing tube, and the diameter of the mixing beads is larger than that of the siphon tube.
4. The integrated multi-reagent sample mixing and loading device of claim 3, wherein: the first puncture block and the second puncture block are arranged in a staggered mode.
5. The integrated multi-reagent sample mixing and loading device of claim 4, wherein: the first isolating film, the second isolating film and the third isolating film are all tinfoil paper.
6. The integrated multi-reagent sample mixing and loading device of claim 5, wherein: the interior of the mixing tube is in a negative pressure state.
7. The integrated multi-reagent sample mixing and loading device of claim 6, wherein: the mixing pipe is internally symmetrically provided with supporting rods, a guide ring is fixedly connected between the two supporting rods, an annular groove is arranged in the guide ring, a rotating ring is arranged in the guide ring, an annular sliding block is arranged outside the rotating ring, the sliding block is positioned in the annular groove, the guide ring and the rotating ring are in rotating fit with the sliding block through the annular groove, and one end, far away from the first isolation film, of the rotating ring is fixedly connected with an inclined mixing rod;
the clamping grooves are symmetrically and fixedly connected to the inner wall of the rotating ring, a plurality of clamping blocks corresponding to the clamping grooves are fixedly connected to one end, away from the collecting body, of the siphon pipe, the clamping blocks can extend into the clamping grooves, and the clamping blocks and the clamping grooves can lock the positions of the siphon pipe and the rotating ring.
8. The integrated multi-reagent sample mixing and loading device of claim 7, wherein: the mixing rod and the second puncture block are arranged in a staggered way.
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