CN1162070C - Method for breeding triploid crucian carp (common carp) and apparatus thereof - Google Patents
Method for breeding triploid crucian carp (common carp) and apparatus thereof Download PDFInfo
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- CN1162070C CN1162070C CNB991152638A CN99115263A CN1162070C CN 1162070 C CN1162070 C CN 1162070C CN B991152638 A CNB991152638 A CN B991152638A CN 99115263 A CN99115263 A CN 99115263A CN 1162070 C CN1162070 C CN 1162070C
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- carp
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The present invention relates to a production method for triploid crucian carp (or common carp), and an apparatus thereof, which is used for the breeding of polyploid fishes. The present invention uses tetraploid fishes as the male parent and uses diploid Japan white crucian carp or diploid common carp as the female parent to carry out artificial fertilization; fertilized eggs are uniformly stuck on a net sheet type artificial fish spawning nest and are suspended in a honeycomb type fry incubator; the fertilized eggs are hatched by slightly flowing water with the dissolved oxygen quantity of 5 to 8 mg/l under the constant temperature of 22 to 24 DEG C, and the hatching rate reaches about 90%; the surface of water per square meter can produce 200 thousand of triploid fish fries. The triploid crucian carp (or common carp) produced by the present invention is completely sterile and has the advantages of fast growth speed, strong disease resistance, delicious meat, low feed coefficient, hypoxia resistance and obvious economic benefit.
Description
The present invention relates to the breeding of fish, be specifically related to production and the equipment thereof of polyploid fish.
Research about artificial induction triploid fish, as far back as the forties the scholar being arranged once was that experiment material has been studied the triploid mechanism of artificial induction with the carp, proposed the dliploid egg nucleus combine with the monoploid sperm nucleus may form the triploid zygote and and then produce the viewpoint of triploid individuality, to inducing the certain reference value of being formed with of fish polyploid afterwards.Swarup (1956,1957,1959) had once carried out the experiment of induced triploid fish continuously, he not only with low temperature induction three quil fishes produce triploid, and raise to sexual maturity.Afterwards, Purdom (1972) had reported seawater economic fish flounder and the success of flounder crossbreed artificial induction triploid again.At present, many in the world countries carry out the research and the practice of fish polyploid fingerling in succession.
Liu Jun etc. have obtained to have obvious heterotic first generation of hybrid F1 in the crossbreeding experiment of red crucian carp of research and Xiangjiang wild carps, find that simultaneously F1 is 4.67% for the male-fertile rate, generally believe that both at home and abroad the carp crucian carp hybridizes male sterile viewpoint thereby revised.On this basis, by directive breeding, therefrom find the male gamete of diploid chromosome, taking this as an opportunity obtains tetraploid fish.The cultivation success of tetraploid fish for utilizing heterosis, hybrid vigor in the fish culture, is produced triploid fish and is laid a good foundation.Yet, the achievement in research of triploid crucian carp (carp) fish is used for production practices, this is China's an open question still.
The present invention is intended to the method for using cell engineering and sexual hybridization to combine, solves the production application problem of triploid crucian carp (carp) fish.
The main technical schemes that realizes the foregoing invention purpose is as follows:
The production method of triploid crucian carp (carp) fish is to be male parent with the tetraploid fish, with dliploid Japanese white crucian or the common carp of dliploid is that female parent carries out artificial insemination, then fertilized egg evenly is attached on the net sheet type artificial fish nest, parallel suspend is in incubator again, under 22～24 ℃ constant temperature, with the miniflow water hatching of dissolved oxygen amount 5-8 mg/litre.The incubator that is exclusively used in hatching triploid crucian carp (carp) fry is a kind of cellular fry hatchibator, the incubator bottom center is respectively equipped with sparge pipe and gas tube, the net sheet type artificial fish nest that has evenly sticked fertilized egg is with the direction perpendicular to sparge pipe and gas tube, parallel suspend is in incubator, and the discharge that enters incubator is 1-2 ton/h.m
3And with 0.2-0.7kg/cm
2Pressure water body inflation in incubator.
Below in conjunction with accompanying drawing in detail the present invention is described in detail.
Fig. 1 is the cross section structure schematic diagram of cellular fry hatchibator.
The production of triploid crucian is to be maternal with the Japanese white crucian, with the tetraploid crucian is male parent, the production of triploid carp is to be female parent with common carp, with the tetraploid carp is male parent, for guaranteeing the production of fry in enormous quantities, adopt the artificial insemination mode, the pond naturality is ripe or be fertilized by the female fish-egg of dliploid and tetraploid sperm that artificial induced spawning reaches sexually matured combinations thereof, and fertilized egg employing cellular fry hatchibator shown in Figure 1 is hatched.Earlier fertilized egg evenly being attached on mesh is on the 40 purpose net sheet type artificial fish nests (4), again the formula fish nest of throwing the net is arranged in parallel and is suspended on the net sheet suspension arrangement (8), spacing between the net sheet is 10cm, the water-bed 10cm of a net pitch of fins, the incubator bottom center is provided with sparge pipe (2), gas tube (3), hydraulic pressure 〉=the 0.1Mpa of sparge pipe ejection, gas tube and air pump join, and air pump is with 0.2～0.7kg/cm
2Pressure water body inflation in incubator, the discharge that enters incubator is 1～2T/h.m
3Water temperature remains on 22～24 ℃, after the sparge pipe ejection of central authorities, flow to the both sides of net sheet, when water level surpasses net sheet 3cm, water is just discharged incubator from low level overflow pipe (6) through Overflow drainage pipeline (12), thereby form the miniflow water of circulation, have the gas of certain pressure from gas tube, to spray, make the dissolved oxygen amount of incubator remain at 5～8mg/L with the micro air bubble shape, it is 60 purpose water outlet screen windows (1) that the incubator both sides are provided with the aperture, spills to stop fry; Confession, the draining gas system of incubator formed in drainage pipeline (9), Overflow drainage ditch and water supply at the bottom of drain cock at the bottom of the pond (5), the pond, feed channel ditch (11), and (10) walk guidance tape for operation, the high-order overflow pipe that (7) are used during for the common fry of hatching.This Design of device is carried out fertilized egg hatching in certain temperature, the sufficient miniflow water environment of oxygen supply are arranged, so incubation rate can reach about 90%, and fry output height, can produce triploid fry 200,000 tails for every square metre.
With triploid crucian carp (carp) fry of this method and device fabrication, chromosome all is triploid (3N=150), and is sterile fully; Fast growth, the triploid crucian is than 2～3 times of the fast growths of general crucian, and the triploid carp is than general carp fast 25～30%; Delicious meat, edible part is many; Resistance against diseases is strong, does not generally catch; Feeding habits are assorted, and the triploid crucian can filter the food plankton, and feed coefficient is low; Lower oxygen concentration resistance; Easily fish for and fish.Have remarkable economic efficiency, be worth large scale application.
Claims (2)
1. the production method of triploid crucian or triploid carp, the production method that it is characterized in that triploid crucian or triploid carp is to be male parent with the tetraploid fish, with dliploid Japanese white crucian or the common carp of dliploid is that female parent carries out artificial insemination, then fertilized egg evenly is attached on the net sheet type artificial fish nest, parallel suspend is in cellular incubator again, under 22～24 ℃ constant temperature, with the miniflow water hatching of dissolved oxygen amount 5-8 mg/litre.
2. one kind is exclusively used in equipment---the cellular fry hatchibator of producing triploid crucian or triploid carp, it is characterized in that the incubator bottom center is respectively equipped with sparge pipe (2) and gas tube (3), the net sheet type artificial fish nest (4) that has evenly sticked fertilized egg is with the direction perpendicular to sparge pipe and gas tube, parallel suspend is on the suspension arrangement (8) of incubator, and the discharge that enters incubator is 1-2 ton/h.m
3, the tracheae pump is with 0.2-0.7kg/cm
2Pressure inflate to incubator through gas tube, be provided with water outlet screen window (1) around the net sheet type artificial fish nest, the position of low level overflow pipe (6) is higher than net sheet type fish nest 3cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB991152638A CN1162070C (en) | 1999-02-15 | 1999-02-15 | Method for breeding triploid crucian carp (common carp) and apparatus thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB991152638A CN1162070C (en) | 1999-02-15 | 1999-02-15 | Method for breeding triploid crucian carp (common carp) and apparatus thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1263703A CN1263703A (en) | 2000-08-23 |
| CN1162070C true CN1162070C (en) | 2004-08-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB991152638A Expired - Lifetime CN1162070C (en) | 1999-02-15 | 1999-02-15 | Method for breeding triploid crucian carp (common carp) and apparatus thereof |
Country Status (1)
| Country | Link |
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| CN (1) | CN1162070C (en) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100382687C (en) * | 2004-08-25 | 2008-04-23 | 中国科学院海洋研究所 | Method for inhibiting primary cleavage of fertilized eggs of paralichthys olivaceus |
| CN101268765B (en) * | 2008-05-15 | 2011-08-17 | 湖北省水产科学研究所 | Fishes oosperm hatcher with all-directional hydrologic cycle |
| CN101822231B (en) * | 2010-05-21 | 2011-11-16 | 云南省水产技术推广站 | Hatching method for Sinocyclocheilus grahami fertilized egg |
| CN102487851A (en) * | 2011-11-18 | 2012-06-13 | 浙江海洋学院 | Keep-alive incubation barrel of fish demersal eggs |
| CN102771430A (en) * | 2012-08-20 | 2012-11-14 | 苏州市苏云生物科技有限公司 | Triploid crucian carp No.2 pond development method |
| CN103918592A (en) * | 2014-05-07 | 2014-07-16 | 娄志 | Crucian fry cultivation method |
| CN104756912A (en) * | 2015-04-01 | 2015-07-08 | 湖南文理学院 | Artificial hatching pond for catfish |
| CN104938386A (en) * | 2015-07-01 | 2015-09-30 | 谢新民 | Inflation oxygenation and water saving type temperature-controllable aquatic animal incubation device |
| CN105994113B (en) * | 2016-06-08 | 2019-01-15 | 江苏省淡水水产研究所 | Fry hatching pond is unpowered water circle device |
| CN107027662A (en) * | 2017-03-24 | 2017-08-11 | 覃忠 | A kind of bar house oil fish hybridization triploid artificial propagation techniques |
| CN110915733B (en) * | 2019-10-16 | 2021-02-02 | 湖南师范大学 | Method for artificial induced spawning, natural mating fertilization and embryo floating running water incubation |
| CN110999830A (en) * | 2020-01-08 | 2020-04-14 | 广西壮族自治区水产引育种中心 | Rapid and efficient carp breeding method |
-
1999
- 1999-02-15 CN CNB991152638A patent/CN1162070C/en not_active Expired - Lifetime
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| Publication number | Publication date |
|---|---|
| CN1263703A (en) | 2000-08-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent for invention or patent application | ||
| CB02 | Change of applicant information |
Co-applicant after: Xiangyin Donghu Fishery |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: CO-APPLICANT TO: EAST LAKE FISHING GROUND, XIANGYIN COUNTY |
|
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Hunan Xiangyun Biological Co., Ltd. Assignor: East Lake fishing ground of Xiangyin County|Hunan Normal University Contract record no.: 2012430000019 Denomination of invention: Method for breeding triploid crucian carp (common carp) and apparatus thereof Granted publication date: 20040818 License type: Exclusive License Open date: 20000823 Record date: 20120322 |
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| CX01 | Expiry of patent term |
Granted publication date: 20040818 |
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| CX01 | Expiry of patent term |