CN116196262A - Preparation method of yeast cell wall extract hydrolysate and cosmetic composition - Google Patents
Preparation method of yeast cell wall extract hydrolysate and cosmetic composition Download PDFInfo
- Publication number
- CN116196262A CN116196262A CN202310088303.2A CN202310088303A CN116196262A CN 116196262 A CN116196262 A CN 116196262A CN 202310088303 A CN202310088303 A CN 202310088303A CN 116196262 A CN116196262 A CN 116196262A
- Authority
- CN
- China
- Prior art keywords
- cell wall
- yeast cell
- hydrolysate
- wall extract
- yeast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000005253 yeast cell Anatomy 0.000 title claims abstract description 173
- 239000000284 extract Substances 0.000 title claims abstract description 99
- 239000002537 cosmetic Substances 0.000 title claims abstract description 77
- 239000000413 hydrolysate Substances 0.000 title claims abstract description 71
- 239000000203 mixture Substances 0.000 title claims abstract description 26
- 238000002360 preparation method Methods 0.000 title abstract description 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 90
- 239000002994 raw material Substances 0.000 claims abstract description 53
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims abstract description 44
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 41
- 239000000126 substance Substances 0.000 claims abstract description 32
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims abstract description 22
- 235000019253 formic acid Nutrition 0.000 claims abstract description 22
- 244000005700 microbiome Species 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 230000003301 hydrolyzing effect Effects 0.000 claims abstract description 12
- 229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 9
- 210000002421 cell wall Anatomy 0.000 claims abstract description 5
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 40
- 239000007788 liquid Substances 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 21
- 230000007062 hydrolysis Effects 0.000 claims description 11
- 238000006460 hydrolysis reaction Methods 0.000 claims description 11
- 239000011259 mixed solution Substances 0.000 claims description 10
- 238000000926 separation method Methods 0.000 claims description 10
- 239000003085 diluting agent Substances 0.000 claims description 9
- 239000012535 impurity Substances 0.000 claims description 9
- 238000002835 absorbance Methods 0.000 claims description 8
- 108010009736 Protein Hydrolysates Proteins 0.000 claims description 5
- 238000000746 purification Methods 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 5
- 239000012498 ultrapure water Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 4
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 241000235058 Komagataella pastoris Species 0.000 claims description 2
- 230000002255 enzymatic effect Effects 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 15
- 230000009965 odorless effect Effects 0.000 abstract description 4
- 238000007865 diluting Methods 0.000 abstract 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 30
- 108010012715 Superoxide dismutase Proteins 0.000 description 30
- 229920000057 Mannan Polymers 0.000 description 25
- 239000000047 product Substances 0.000 description 22
- 210000003491 skin Anatomy 0.000 description 22
- 229920002498 Beta-glucan Polymers 0.000 description 18
- 229920001503 Glucan Polymers 0.000 description 14
- 241000196324 Embryophyta Species 0.000 description 13
- 150000003839 salts Chemical class 0.000 description 13
- 229920001285 xanthan gum Polymers 0.000 description 12
- 239000000230 xanthan gum Substances 0.000 description 12
- 229940082509 xanthan gum Drugs 0.000 description 12
- 235000010493 xanthan gum Nutrition 0.000 description 12
- LUEWUZLMQUOBSB-GFVSVBBRSA-N mannan Chemical class O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@H]3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-GFVSVBBRSA-N 0.000 description 11
- 230000000694 effects Effects 0.000 description 10
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 9
- 239000005913 Maltodextrin Substances 0.000 description 8
- 229920002774 Maltodextrin Polymers 0.000 description 8
- 229940035034 maltodextrin Drugs 0.000 description 8
- 229920002101 Chitin Polymers 0.000 description 7
- 150000004676 glycans Chemical class 0.000 description 6
- 239000004006 olive oil Substances 0.000 description 6
- 235000008390 olive oil Nutrition 0.000 description 6
- 229920001282 polysaccharide Polymers 0.000 description 6
- 239000005017 polysaccharide Substances 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 239000003513 alkali Substances 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 230000004071 biological effect Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 150000002632 lipids Chemical class 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 3
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 229910052802 copper Inorganic materials 0.000 description 3
- 239000010949 copper Substances 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 230000003834 intracellular effect Effects 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000004302 potassium sorbate Substances 0.000 description 3
- 235000010241 potassium sorbate Nutrition 0.000 description 3
- 229940069338 potassium sorbate Drugs 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 239000011701 zinc Substances 0.000 description 3
- 229910052725 zinc Inorganic materials 0.000 description 3
- 229920001661 Chitosan Polymers 0.000 description 2
- 229920002261 Corn starch Polymers 0.000 description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 2
- 244000000231 Sesamum indicum Species 0.000 description 2
- 235000003434 Sesamum indicum Nutrition 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000012993 chemical processing Methods 0.000 description 2
- 239000008120 corn starch Substances 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- 230000002526 effect on cardiovascular system Effects 0.000 description 2
- 239000003995 emulsifying agent Substances 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 230000031700 light absorption Effects 0.000 description 2
- 239000003208 petroleum Substances 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000029663 wound healing Effects 0.000 description 2
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 102100024023 Histone PARylation factor 1 Human genes 0.000 description 1
- DUKURNFHYQXCJG-UHFFFAOYSA-N Lewis A pentasaccharide Natural products OC1C(O)C(O)C(C)OC1OC1C(OC2C(C(O)C(O)C(CO)O2)O)C(NC(C)=O)C(OC2C(C(OC3C(OC(O)C(O)C3O)CO)OC(CO)C2O)O)OC1CO DUKURNFHYQXCJG-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102000003425 Tyrosinase Human genes 0.000 description 1
- 108060008724 Tyrosinase Proteins 0.000 description 1
- 229920000392 Zymosan Polymers 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000005859 cell recognition Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000033629 detection of yeast Effects 0.000 description 1
- 238000004033 diameter control Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 150000008131 glucosides Chemical group 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 108010005335 mannoproteins Proteins 0.000 description 1
- 125000000311 mannosyl group Chemical group C1([C@@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 230000037067 skin hydration Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a preparation method of yeast cell wall extract hydrolysate and a cosmetic composition, wherein the yeast cell wall is obtained by breaking wall and separating the used yeast raw material, most of color, smell and inorganic salt in the cell wall are removed by repeatedly diluting with water, the clean crude yeast cell wall is obtained by separating and concentrating, the color, smell and inorganic salt in the cell wall are further removed by hydrolyzing the clean crude yeast cell wall by acetic acid, the yeast cell wall extract is obtained, the solubility of the yeast cell wall extract is increased by hydrolyzing the yeast cell wall extract by formic acid, the yeast cell wall extract hydrolysate which is colorless, odorless, salt-free and soluble and retains substances beneficial to skin protection in the yeast cell wall is obtained, and the obtained yeast cell wall extract hydrolysate is used as a main component, and other components which are mainly derived from cosmetic raw materials of microorganisms and/or plants are used to form a novel cosmetic composition.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and in particular relates to a preparation method of a yeast cell wall extract hydrolysate and a cosmetic composition.
Background
The yeast cell wall accounts for about 20% -30% of the dry weight of the whole cell, has the important functions of maintaining cell morphology and identifying among cells, is divided into 4 layers according to the structure, wherein the inner layer is a glucan layer, the middle layer mainly consists of proteins, the outer layer is a mannan layer, the innermost layer is chitin, and the layers can be partially inlaid; according to chemical composition, the mannans account for about 30% of dry weight of yeast cell walls, the beta-glucans account for about 30%, the glycoproteins and the chitin account for about 20%, and other components such as proteins, lipids, inorganic salts and the like account for about 20%.
The beta-glucan is a structural polysaccharide connected with a plasma membrane and forms a main component of a yeast cell wall, and has the function of supporting external mannose, wherein the beta-glucan consists of beta-1, 3-glucan and beta-1, 6-glucan, and the ratio of the beta-glucan to the beta-glucan is 85:15. Beta-glucan takes beta-1, 3-glucan as a framework, beta-1, 6-glucan as a branched chain and chitin as the innermost layer.
The average molecular weight of the beta-1, 3-glucan is 240kDa, the average molecular weight of the beta-1, 6-glucan is 24kDa, the average molecular weight of the mannans is 150kDa, and the average molecular weight of the chitin is 25kDa.
The mannans have various physiological functions of cell recognition, cell wall pore diameter control and the like, the mannans are connected with proteins together through covalent bonds, the main chain is single-chain, a plurality of mannans are connected through alpha-1, 6-glycosidic bonds to form, the mannose side chains are connected with the main chain through alpha-1, 2 and alpha-1, 3 bonds, and partial side chains are combined with functional groups which determine the correlation of yeast cell antigens.
The two functions now identified for mannans in the yeast cell wall are adsorption of pathogenic bacteria and modulation of immunity.
Mannans are widely used in cosmetics, which have the effects of increasing the viscosity of aqueous solutions, stabilizing emulsions, moisturizing, and also activating skin and anti-inflammatory properties.
Mannoproteins of the yeast cell wall have the same hydrophilic and lipophilic structure as surfactants and many emulsifiers and can act as emulsifiers in cosmetics.
Yeast glucan is immune polysaccharide existing in natural yeast cell walls, has antitumor activity for the first time in 1963, has been found to have outstanding performances in terms of antibacterial, immunoregulation, radiation resistance, gastrointestinal regulation, tissue structure regeneration or repair assistance, wound healing promotion, cardiovascular and cerebrovascular prevention, diabetes prevention and the like, has unique biological activity in terms of tumors, hepatitis, cardiovascular, diabetes, blood lipid reduction, aging resistance and the like, is the first glucan found to have the immunological activity, is widely used in cosmetics, and mainly has the functions of film formation, adhesive, softener, skin conditioning and the like in cosmetics.
The basic unit of chitin is acetylglucosamine, which is a polymer formed by connecting 1000-3000 acetylglucosamine residues with each other through 1,4 glucoside chains.
Chitin can be hydrolyzed into chitosan, and can be used as humectant and skin conditioner in cosmetics, and has effects of accelerating wound healing, improving skin hydration degree, reducing skin fine lines, improving rough skin, resisting tyrosinase synthesis, reducing melanin generation, improving uneven skin color, and whitening skin.
Also in the yeast cell wall are proteins, lipids and other substances beneficial to the growth of skin cells, which exert beneficial effects on skin and on growth metabolism.
The protein can generate a large amount of polypeptide after hydrolysis, and the polypeptide has a large amount of application in cosmetics, and has special effects on protecting, repairing and treating skin.
Many of the lipids have particular effects on protecting the skin.
As described above, yeast cell walls contain a large amount of useful substances for cosmetics, but yeast produces yellow or brown-yellow substances during fermentation culture and also produces a special smell, which seriously affects the quality of cosmetics when used as raw materials for cosmetics, and also contains inorganic salts and other substances harmful to skin in the yeast cell walls, so that it is necessary to remove the color and smell substances and inorganic salts to produce yeast cell wall extracts suitable for application to cosmetics.
Further, as described above, yeast polysaccharides include β -glucan and α -mannan, the average molecular weight of β -1, 3-glucan is 240kDa, the average molecular weight of β -1, 6-glucan is 24kDa, the average molecular weight of mannan is 150kDa, and the average molecular weight of chitin is 25kDa, and these macromolecular polysaccharides are hardly miscible with water and are important indicators when used as raw materials for cosmetics, so that improvement of the solubility is very important for its application in the cosmetic field.
In the existing methods for producing zymosan, mainly a method of combining alkali and enzyme, a method of combining alkali and salt, a method of combining alkali and acid, a method of combining alkali and organic solvent, a method of combining acid and salt and the like are adopted, and generally, products produced by the methods are single yeast cell wall polysaccharide, such as beta-glucan and alpha-mannan, a large amount of substances beneficial to skin care in the yeast cell wall are destroyed and lost by the methods, the functions of the produced products in cosmetic application are reduced, the utilization rate of yeast cell wall raw materials is greatly reduced due to the single products, the yield of the products is reduced, the destroyed and lost substances also cause environmental pollution, and the production cost of the products is increased due to the single products produced by the methods.
The existing products for producing polysaccharide by using yeast walls, mainly beta-glucan and alpha-manna, have single effects due to single products, and can be seen from the components in the yeast walls, wherein a large amount of substances with special effects on skin protection, repair and treatment are included in addition to the beta-glucan and the alpha-manna.
Therefore, a method for preparing a yeast cell wall extract hydrolysate which can retain most of the components beneficial to cosmetic application in the yeast cell wall and can remove substances and inorganic salts containing color and smell in the yeast cell wall, produce colorless, odorless, salt-free and easily soluble substances beneficial to skin protection in the yeast cell wall, and a cosmetic composition which takes the yeast cell wall extract hydrolysate as a main component and takes the cosmetic raw materials derived from microorganisms and/or plants as main other components is found, and plays a vital role in the application of the yeast cell wall extract hydrolysate, particularly in the application of the yeast cell wall extract hydrolysate as cosmetic raw materials.
Disclosure of Invention
The present invention aims at finding a method for preparing yeast cell wall extract hydrolysate which can retain most of components beneficial to cosmetic application in yeast cell walls, can remove substances and inorganic salts containing color and smell in the yeast cell walls, produce colorless, odorless, salt-free and easily soluble substances beneficial to skin protection in most of yeast cell walls, and provides a novel cosmetic composition which is formed by using the yeast cell wall extract hydrolysate as a main component and using cosmetic raw materials derived from microorganisms and/or plants as main other components.
The aim of the invention is realized by the following technical scheme:
a method for preparing a yeast cell wall extract hydrolysate, comprising the steps of:
(1) Adding water into the yeast raw material, uniformly mixing to obtain yeast diluent, and separating the yeast diluent by a separator to remove liquid part and obtain yeast solid;
(2) Repeating the step (1) to obtain a clean yeast raw material;
(3) Breaking the wall of clean yeast raw materials to obtain yeast wall-broken liquid;
(4) Separating the yeast wall-broken liquid by using a separator, and separating and removing a liquid part to obtain a crude yeast cell wall;
(5) Adding water to the crude yeast cell wall obtained by separation, and uniformly mixing to obtain crude yeast cell wall diluent;
(6) Separating the crude yeast cell wall diluent again by using a separator to remove liquid, thereby obtaining the crude yeast cell wall which is cleaned for the first time;
(7) Repeating the steps (4) - (6), and primarily removing color, smell and other impurities by the repeated dilution and separation purification method to obtain purified crude yeast cell walls;
(8) Adding acetic acid into the clean crude yeast cell wall to obtain a clean crude yeast cell wall and acetic acid mixed solution;
(9) Hydrolyzing the clean crude yeast cell wall and acetic acid mixed solution, and further hydrolyzing color and odor substances and other impurities which can be dissolved by acetic acid in the clean crude yeast cell wall to obtain clean crude yeast cell wall acetic acid hydrolysate;
(10) Separating the clean crude yeast cell wall acetic acid hydrolysate by using a separator, and removing acetic acid liquid to obtain a precipitated crude yeast cell wall extract;
(11) Removing acetic acid from the crude yeast cell wall extract to obtain a precipitated yeast cell wall extract;
(12) Adding formic acid into the yeast cell wall extract to obtain a yeast cell wall extract and formic acid mixed solution;
(13) Hydrolyzing the yeast cell wall extract and formic acid mixed solution to obtain yeast cell wall extract formic acid hydrolysate;
(14) Removing formic acid from the formic acid hydrolysate of the yeast cell wall extract to obtain yeast cell wall extract hydrolysate.
The yeasts in the step (1) include Saccharomyces cerevisiae, pichia pastoris, etc.
The wall breaking in the step (3) comprises mechanical wall breaking, chemical wall breaking, enzymatic wall breaking and the like.
And (3) hydrolyzing the clean crude yeast cell wall and acetic acid mixed solution in the step (9), wherein the hydrolysis temperature is not lower than 65 ℃ and the hydrolysis time is not less than 1 hour.
The acetic acid liquid described in step (10) includes color and odor materials and other impurities that may be dissolved by acetic acid in the clean crude yeast cell wall.
And (3) hydrolyzing the yeast cell wall extract and formic acid mixed solution in the step (13), wherein the hydrolysis temperature is not lower than 70 ℃ and the hydrolysis time is not less than 0.5 hour.
A cosmetic composition comprising a yeast cell wall extract hydrolysate as a main component, characterized by:
(1) The viscosity value of the yeast cell wall extract hydrolysate is not more than 500cP;
(2) The absorbance of the yeast cell wall extract hydrolysate is not more than 0.05;
(3) The inorganic salt content in the hydrolysate of the yeast cell wall extract is less than 500ppm;
(4) The formic acid content in the hydrolysate of the yeast cell wall extract is less than 200ppm;
(5) The yeast cell wall extract hydrolysate accounts for not less than 20% of the cosmetic composition;
(6) The cosmetic composition includes other components.
The viscosity number described in feature (1) was measured using a viscosity tester at 20℃and a dry matter content of the yeast cell wall extract hydrolysate of 35%, calculated as the percentage of the dry matter weight of the yeast cell wall extract hydrolysate to the total weight of the yeast cell wall extract hydrolysate.
The absorbance in the feature (2) was measured spectrophotometrically by measuring the absorbance of the yeast cell wall extract hydrolysate obtained at a wavelength of 420nm against ultrapure water.
The inorganic salt content described in feature (3) is calculated using the ash content of the yeast cell wall extract hydrolysate, based on the percentage of the weight of ash content in the yeast cell wall extract hydrolysate to the total weight of dry matter of the yeast cell wall extract hydrolysate.
The proportion of not less than 20% in the feature (5) is calculated on the basis of the weight percentage of the yeast cell wall extract hydrolysate based on the total weight of the cosmetic composition, excluding water.
The ratio of the microorganism-derived and/or plant-derived cosmetic raw material in the other components described in the feature (6) is more than 90%, and the microorganism-derived and/or plant-derived cosmetic raw material means a cosmetic raw material extracted and purified from the microorganism, and the plant-derived cosmetic raw material means a cosmetic raw material extracted and purified from the plant, based on the weight of the microorganism-derived and/or plant-derived cosmetic raw material as a percentage of the total weight of the other components excluding water.
The invention has the advantages and beneficial effects that:
1. the product produced by the present invention (yeast cell wall extract hydrolysate) is a completely new product, and none of the existing patents and documents is presented with the same product as the product produced by the present invention.
2. The product (yeast cell wall extract hydrolysate) produced by the invention retains most substances beneficial to skin protection in the yeast cell wall, and increases other beneficial components in the yeast cell wall, such as chitosan, protein, lipoid and the like, while retaining the products produced by the prior production technology, such as yeast glucan and mannans, thereby improving the application effect of the produced products in the cosmetic field.
3. As the product of the invention retains most substances beneficial to skin protection in the yeast cell wall, compared with the prior art, the invention improves the utilization rate of the yeast cell wall raw material, improves the yield of the product and reduces the production cost.
4. The product of the invention keeps most substances beneficial to skin protection in the yeast cell wall, improves the utilization rate of the yeast cell wall raw material, and the added alkali and enzyme in the preparation method of the prior art hydrolyze and discharge all other substances except glucan and mannan in the yeast cell wall, so that the cost of sewage treatment is increased and the environment is polluted.
5. The preparation method adopts simple process steps, mainly utilizes acetic acid hydrolysis, removes yellow or brown color and smell substances generated in the processes of culturing and fermenting yeast, reduces the production cost and improves the application effect of the produced product in the field of cosmetics.
6. According to the preparation method, the solubility of the yeast cell wall extract is increased by adopting the hydrolysis of formic acid to the yeast cell wall extract, and the solubility of the yeast cell wall extract hydrolysate can be flexibly controlled according to the requirements of products by controlling the hydrolysis temperature and time, so that the application effect of the produced products in the field of cosmetics is improved.
7. In cosmetics, the viscosity (also representing the hydrolysis degree) of the added raw materials is a very important index, and the viscosity directly influences the quality of the cosmetics.
8. In cosmetics, the color can influence the quality of the cosmetics, the invention defines the light absorption value (representing the color) of the yeast cell wall extract hydrolysate in the characteristic (2) of the cosmetic composition, and when the light absorption value is not more than 0.05, the color of the yeast cell wall extract hydrolysate is a colorless transparent liquid, and the quality of the cosmetics produced by the yeast cell wall extract hydrolysate can be improved by visually inspecting the color of the yeast cell wall extract hydrolysate.
9. According to the invention, the contents of inorganic salt and formic acid in the yeast cell wall extract hydrolysate are respectively defined in the characteristics (3) and (4), the inorganic salt and the formic acid can stimulate skin, and the quality of cosmetics produced by utilizing the yeast cell wall extract hydrolysate can be improved due to the reduction of the contents.
10. In the existing cell wall extract, the product is single, the effect in cosmetics is little, the addition proportion in the cosmetic composition is little, and the yeast cell wall extract hydrolysate produced by the invention keeps most substances beneficial to skin protection in the yeast cell wall, removes color and smell substances and inorganic salts harmful to skin, increases the solubility of the substances and inorganic salts, and increases the use amount of the substances in the cosmetic composition.
11. In the existing cosmetic composition, wherein the weight proportion of the raw materials of the cosmetics derived from microorganisms and/or plants is relatively low in the total weight of the cosmetic composition, the cosmetics truly derived from microorganisms and/or plants cannot be produced, and the main component derived from microorganisms used in the present invention is a pure, colorless, odorless, salt-free yeast cell wall extract hydrolysate which retains most of substances beneficial for skin protection, repair and treatment in yeast cell walls, so that the raw materials can be added in any proportion as required, and other components in the cosmetic composition are defined in the feature (6), so that the present invention is truly a cosmetic composition derived from microorganisms and/or plants.
Drawings
In order that the present invention may be more clearly understood, the following description will be made in detail with reference to the accompanying drawings, wherein fig. 1 is a flow chart of a preparation process according to the present invention.
Detailed Description
The invention will be further described by means of specific examples.
Example 1
(1) Taking 800g of waste yeast (dry matter content is 33%) which is a byproduct of beer fermentation, adding 4000g of water, setting a separator to 5000 rpm, separating for 15 minutes with a separation factor 4472, and removing supernatant to obtain 784g of yeast solid (dry matter content is 33%) at the bottom;
(2) Adding 4000g of water into yeast solid, stirring uniformly, setting a separator to 5000 rpm, separating for 15 minutes with a separation factor 4472, and removing upper liquid to obtain 780g of yeast solid (dry matter content 33%);
(3) Repeating step (2) 5 times to obtain 764g (dry matter content 33%) of clean yeast raw material;
(4) Adding 1000g of water into a clean yeast raw material, uniformly stirring, then breaking the wall of the clean yeast raw material by using a homogenizer, wherein the pressure of the homogenizer is set to 130MPa, the flow is 55ml/min, and the circulation times are 5 times to obtain a yeast wall breaking liquid;
(5) Separating the yeast cell wall breaking liquid, setting the rotating speed of a separator at 5000 rpm, setting the separating factor at 5411, separating for 15 minutes, and separating and removing the liquid part to obtain 320g of crude yeast cell wall (dry matter content is 33%);
(6) Adding 4000g of water to the crude yeast cell wall obtained by separation, stirring uniformly, then separating again, and removing liquid to obtain 316g of crude yeast cell wall (the dry matter content is 33%);
(7) Repeating steps (5) and (6) for 5 times, and removing most of color and odor substances, inorganic salts and other impurities by the separation and cleaning to obtain 300g of clean crude yeast cell wall (the dry matter content is 33 percent);
(8) Adding 4000g of acetic acid into the clean crude yeast cell wall, performing preliminary hydrolysis at the temperature of 100 ℃ for 3 hours, further hydrolyzing color and odor substances and other impurities which can be dissolved by the acetic acid in the clean crude yeast cell wall, and further releasing inorganic salts in the impurities to obtain clean crude yeast cell wall acetic acid hydrolysate;
(9) Separating the clean crude yeast cell wall acetic acid hydrolysate, setting the rotating speed of a separator at 5500 r/min, setting the separating factor at 5411, separating for 15 minutes, and removing acetic acid liquid to obtain 264g (dry matter content of 34%) of precipitated crude yeast cell wall extract;
(10) 1600g of water is added to the precipitated crude yeast cell wall extract obtained by separation, and a precipitated crude yeast cell wall extract diluent is obtained;
(11) Separating the precipitated crude yeast cell wall extract dilution at 5500 rpm with a separation factor of 5411 for 15 minutes to remove liquid to obtain 252g (dry matter content 34%) of precipitated yeast cell wall extract;
(12) 2400g of formic acid is added into the yeast cell wall extract, hydrolysis is carried out at the temperature of 100 ℃ for 5 hours, and a formic acid hydrolysate of the yeast cell wall extract is obtained;
(13) Formic acid was removed from the liquid by repeated evaporation with water, and 244g (35% dry matter) of decolorized, deodorized, desalted and soluble yeast cell wall extract hydrolysate was finally obtained.
Detection of yeast cell wall extract hydrolysate:
color: the absorbance of the yeast cell wall extract hydrolysate obtained at 420nm wavelength was measured by spectrophotometry using an ultraviolet-visible spectrophotometer manufactured by INESA company, model L5S and purified water as a control, and the absorbance was found to be 0.03.
Colorless by visual inspection.
Smell: the yeast cell wall extract hydrolysate was free of the specific odor of yeast as determined by sensory testing.
And (3) viscosity detection: the viscosity tester is adopted for detection, the device is a viscosity tester, the model is NDJ-5S, and the detection result is 285 cP at 20 ℃.
Determination of inorganic salts: the result of the detection was 0.076S/m using a conductivity meter (Lei Ci desk type conductivity meter DDS-307A), and the salt content in the yeast cell wall extract hydrolysate was 378ppm.
Example 2
(1) 30g (dry matter content 35%) of the yeast cell wall extract hydrolysate obtained in example 1 was taken and put into a graduated 100ml beaker.
(2) Taking 150mg of superoxide dismutase (SOD) with the biological activity of 7500U/mg, wherein the adopted superoxide dismutase is intracellular enzyme biosynthesized in vivo by utilizing yeast, and then obtaining blue-green crystals through wall breaking, filtering, purifying and freeze drying, the superoxide dismutase (SOD) is copper-containing and zinc-containing superoxide dismutase, and adding the 150mg of superoxide dismutase (SOD) into the beaker in the step (1).
(3) Taking 0.5g of xanthan gum, adding the xanthan gum into a beaker in the step (1), and obtaining the xanthan gum through microbial synthesis, extraction and purification.
(4) 2g of glucan is taken and added into the beaker in the step (1), and the glucan is processed by using yeast cell walls as raw materials.
(5) Taking 2g of mannan, adding the mannan into a beaker in the step (1), wherein the mannan is processed by using yeast cell walls as raw materials.
(6) Adding ultrapure water into the beaker in the step (1), adding the beaker to the position with the scale of 100ml, and uniformly stirring to obtain the cosmetic which takes the yeast cell wall extract hydrolysate as the main component and takes superoxide dismutase (SOD), xanthan gum, glucan and mannans as other components and all components are derived from microorganisms.
Example 3
(1) 35g of the yeast cell wall extract hydrolysate obtained in example 1 (dry matter content: 35%) was taken and put into a graduated 100ml beaker.
(2) Taking 200mg of superoxide dismutase (SOD) with the biological activity of 7500U/mg, wherein the adopted superoxide dismutase is intracellular enzyme biosynthesized in vivo by utilizing yeast, and then obtaining blue-green crystals through wall breaking, filtering, purifying and freeze drying, the superoxide dismutase (SOD) is copper-containing and zinc-containing superoxide dismutase, and adding the 200mg of superoxide dismutase (SOD) into the beaker in the step (1).
(3) Taking 0.3g of xanthan gum, adding the xanthan gum into a beaker in the step (1), and obtaining the xanthan gum through microbial synthesis, extraction and purification.
(4) Adding 1.5g of glucan into the beaker in the step (1), wherein the glucan is processed by using yeast cell walls as raw materials.
(5) Taking 1.5g of mannan, adding the mannan into a beaker in the step (1), and processing the mannan by using yeast cell walls as raw materials.
(6) Taking 2g of maltodextrin, and adding the maltodextrin into a beaker in the step (1), wherein the maltodextrin is obtained by processing corn starch serving as a raw material.
(7) Adding 1.5g of olive oil into the beaker in the step (1), wherein the adopted olive oil is obtained by processing sesame serving as a raw material.
(8) Adding ultrapure water into the beaker in the step (1), adding the beaker to the position with the scale of 100ml, and uniformly stirring to obtain the cosmetic which takes yeast cell wall extract hydrolysate as a main component and takes microorganism-derived cosmetic raw materials such as superoxide dismutase (SOD), xanthan gum, glucan and mannans and plant-derived cosmetic raw materials such as maltodextrin and olive oil as other components.
Example 4
(1) 40g (dry matter content 35%) of the yeast cell wall extract hydrolysate obtained in example 1 was taken and put into a graduated 100ml beaker.
(2) Taking 250mg of superoxide dismutase (SOD) with the biological activity of 7500U/mg, wherein the adopted superoxide dismutase is intracellular enzyme biosynthesized in vivo by utilizing yeast, and then obtaining blue-green crystals through wall breaking, filtering, purifying and freeze drying, the superoxide dismutase (SOD) is copper-containing and zinc-containing superoxide dismutase, and adding the 250mg of superoxide dismutase (SOD) into the beaker in the step (1).
(3) Taking 0.2g of xanthan gum, adding the xanthan gum into a beaker in the step (1), and obtaining the xanthan gum through microbial synthesis, extraction and purification.
(4) Adding 1.5g of glucan into the beaker in the step (1), wherein the glucan is processed by using yeast cell walls as raw materials.
(5) Taking 1.5g of mannan, adding the mannan into a beaker in the step (1), and processing the mannan by using yeast cell walls as raw materials.
(6) Taking 1.8g of maltodextrin, and adding the maltodextrin into a beaker in the step (1), wherein the maltodextrin is obtained by processing corn starch serving as a raw material.
(7) Adding 1.5g of olive oil into the beaker in the step (1), wherein the adopted olive oil is obtained by processing sesame serving as a raw material.
(8) Adding 0.03g of potassium sorbate into the beaker in the step (1), wherein the potassium sorbate is obtained by using petroleum as a raw material and performing chemical processing.
(9) Adding 0.02g of essence into the beaker in the step (1), wherein the essence is obtained by using petroleum as a raw material and performing chemical processing.
(10) Adding ultrapure water into the beaker in the step (1), adding the beaker to the position with the scale of 100ml, and uniformly stirring to obtain cosmetics which take yeast cell wall extract hydrolysate as main components, and take plant-derived cosmetic raw materials such as superoxide dismutase (SOD), xanthan gum, glucan and mannans, plant-derived cosmetic raw materials such as maltodextrin and olive oil and chemical-derived cosmetic raw materials such as potassium sorbate and essence as other components, wherein the weight proportion of the chemical-derived cosmetic raw materials is 0.73 percent of the total weight of the other components and is less than 10 percent defined in the cosmetic composition.
Claims (13)
1. A method for preparing a yeast cell wall extract hydrolysate, comprising the steps of:
(1) Adding water into the yeast raw material, uniformly mixing to obtain yeast diluent, and separating the yeast diluent by a separator to remove liquid part and obtain yeast solid;
(2) Repeating the step (1) to obtain a clean yeast raw material;
(3) Breaking the wall of clean yeast raw materials to obtain yeast wall-broken liquid;
(4) Separating the yeast wall-broken liquid by using a separator, and separating and removing a liquid part to obtain a crude yeast cell wall;
(5) Adding water to the crude yeast cell wall obtained by separation, and uniformly mixing to obtain crude yeast cell wall diluent;
(6) Separating the crude yeast cell wall diluent again by using a separator to remove liquid, thereby obtaining the crude yeast cell wall which is cleaned for the first time;
(7) Repeating the steps (4) - (6), and primarily removing color, smell and other impurities by the repeated dilution and separation purification method to obtain purified crude yeast cell walls;
(8) Adding acetic acid into the clean crude yeast cell wall to obtain a clean crude yeast cell wall and acetic acid mixed solution;
(9) Hydrolyzing the clean crude yeast cell wall and acetic acid mixed solution, and further hydrolyzing color and odor substances and other impurities which can be dissolved by acetic acid in the clean crude yeast cell wall to obtain clean crude yeast cell wall acetic acid hydrolysate;
(10) Separating the clean crude yeast cell wall acetic acid hydrolysate by using a separator, and removing acetic acid liquid to obtain a precipitated crude yeast cell wall extract;
(11) Removing acetic acid from the crude yeast cell wall extract to obtain a precipitated yeast cell wall extract;
(12) Adding formic acid into the yeast cell wall extract to obtain a yeast cell wall extract and formic acid mixed solution;
(13) Hydrolyzing the yeast cell wall extract and formic acid mixed solution to obtain yeast cell wall extract formic acid hydrolysate;
(14) Removing formic acid from the formic acid hydrolysate of the yeast cell wall extract to obtain yeast cell wall extract hydrolysate.
2. The yeast of step (1) of claim 1, wherein the yeast comprises Saccharomyces cerevisiae, pichia pastoris, and the like.
3. The method of claim 1, wherein the breaking of the cell wall in step (3) comprises mechanical breaking, chemical breaking, enzymatic breaking, etc.
4. The method of claim 1, wherein the step (9) is performed by hydrolyzing the purified crude yeast cell wall and acetic acid mixture at a temperature of not less than 65 ℃ for not less than 1 hour.
5. The acetic acid liquid of step (10) of claim 1, wherein the acetic acid liquid comprises color and odor materials and other impurities soluble in acetic acid in the clean crude yeast cell wall.
6. The method according to claim 1, wherein the hydrolysis is carried out at a temperature of not less than 70℃for not less than 0.5 hours.
7. A cosmetic composition comprising a yeast cell wall extract hydrolysate as a main component, characterized by:
(1) The viscosity value of the yeast cell wall extract hydrolysate is not more than 500cP;
(2) The absorbance of the yeast cell wall extract hydrolysate is not more than 0.05;
(3) The inorganic salt content in the hydrolysate of the yeast cell wall extract is less than 500ppm;
(4) The formic acid content in the hydrolysate of the yeast cell wall extract is less than 200ppm;
(5) The yeast cell wall extract hydrolysate accounts for not less than 20% of the cosmetic composition;
(6) The cosmetic composition includes other components.
8. The viscosity number of claim 7, wherein the viscosity number is measured by a viscosity tester at 20℃and the dry matter content of the yeast cell wall extract hydrolysate is 35%, calculated as a percentage of the dry matter weight of the yeast cell wall extract hydrolysate to the total weight of the yeast cell wall extract hydrolysate.
9. The method according to claim 7, wherein the absorbance is measured spectrophotometrically by measuring the absorbance of a yeast cell wall extract hydrolysate obtained at a wavelength of 420nm against ultrapure water.
10. The inorganic salt content of claim 7, wherein the inorganic salt content is calculated from ash content of the yeast cell wall extract hydrolysate as a percentage of the weight of ash content in the yeast cell wall extract hydrolysate based on the total weight of dry matter of the yeast cell wall extract hydrolysate.
11. The composition according to claim 7, wherein the proportion of the yeast cell wall extract is not less than 20%, and wherein the proportion of the yeast cell wall extract is not less than 20% based on the weight of the yeast cell wall extract and the total weight of the cosmetic composition, excluding water.
12. The other component according to claim 7, wherein the proportion of the cosmetic raw material derived from the microorganism and/or the plant in the other component is more than 90%, based on the weight of the cosmetic raw material derived from the microorganism and/or the plant in the total weight of the other component, excluding water.
13. The cosmetic raw material derived from a microorganism and/or a plant according to claim 9, wherein the cosmetic raw material derived from a microorganism is a cosmetic raw material extracted and purified from a microorganism, and the cosmetic raw material derived from a plant is a cosmetic raw material extracted and purified from a plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310088303.2A CN116196262A (en) | 2023-02-09 | 2023-02-09 | Preparation method of yeast cell wall extract hydrolysate and cosmetic composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310088303.2A CN116196262A (en) | 2023-02-09 | 2023-02-09 | Preparation method of yeast cell wall extract hydrolysate and cosmetic composition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116196262A true CN116196262A (en) | 2023-06-02 |
Family
ID=86518539
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310088303.2A Pending CN116196262A (en) | 2023-02-09 | 2023-02-09 | Preparation method of yeast cell wall extract hydrolysate and cosmetic composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116196262A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116159002A (en) * | 2023-02-09 | 2023-05-26 | 北京活力蓝晶生物科技有限公司 | Production process of yeast cell wall extract hydrolysate |
| WO2024164773A1 (en) * | 2023-02-09 | 2024-08-15 | 北京活力蓝晶生物科技有限公司 | Production process for producing yeast cell wall extract hydrolysate using organic acids |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583802A (en) * | 2004-06-02 | 2005-02-23 | 中国农业大学 | Method for extracting beta-1,3-dextran |
| JP2007045714A (en) * | 2005-08-05 | 2007-02-22 | Kirin Brewery Co Ltd | Composition for ameliorating skin condition |
-
2023
- 2023-02-09 CN CN202310088303.2A patent/CN116196262A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583802A (en) * | 2004-06-02 | 2005-02-23 | 中国农业大学 | Method for extracting beta-1,3-dextran |
| JP2007045714A (en) * | 2005-08-05 | 2007-02-22 | Kirin Brewery Co Ltd | Composition for ameliorating skin condition |
Non-Patent Citations (1)
| Title |
|---|
| 俞学锋等: "《生物化学实验 第2版》", vol. 2, 电子科技大学出版社, pages: 115 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116159002A (en) * | 2023-02-09 | 2023-05-26 | 北京活力蓝晶生物科技有限公司 | Production process of yeast cell wall extract hydrolysate |
| WO2024164773A1 (en) * | 2023-02-09 | 2024-08-15 | 北京活力蓝晶生物科技有限公司 | Production process for producing yeast cell wall extract hydrolysate using organic acids |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wang et al. | Ultrasonic treatment for improved solution properties of a high-molecular weight exopolysaccharide produced by a medicinal fungus | |
| Hifney et al. | Upgrading the antioxidant properties of fucoidan and alginate from Cystoseira trinodis by fungal fermentation or enzymatic pretreatment of the seaweed biomass | |
| CN116196262A (en) | Preparation method of yeast cell wall extract hydrolysate and cosmetic composition | |
| CN114901701A (en) | Ultra-low molecular weight hyaluronic acid and preparation method thereof | |
| CN117925731B (en) | Galactose yeast-like fungus centella asiatica fermentation product and preparation method and application thereof | |
| US20100221382A1 (en) | Cell wall derivatives, their preparation process, and use thereof | |
| JPH0348201B2 (en) | ||
| US10590207B2 (en) | Process for isolating fucoidan and laminarin from live, harvested seaweed | |
| JP5732390B2 (en) | Method for the co-production of chitin, its derivatives and polymers containing glucose, mannose and / or galactose by fermentation of yeast Pichia pastoris | |
| KR101132114B1 (en) | Method of molecular weight control of hyaluronic acid | |
| CN113755544A (en) | Schizophyllum commune fermentation product and preparation method and application thereof | |
| CN111741963A (en) | Low-molecular-weight chondroitin sulfate and preparation method thereof | |
| Liang et al. | Flash extraction of ulvan polysaccharides from marine green macroalga Ulva linza and evaluation of its antioxidant and gut microbiota modulation activities | |
| CN116159002A (en) | Production process of yeast cell wall extract hydrolysate | |
| CN116370360B (en) | Production process for producing yeast cell wall extract hydrolysate by using organic acid | |
| CN1609188A (en) | A strain for producing pullulan and a method for producing pullulan using the strain | |
| CN110627863B (en) | Proteoglycan extraction method, extract and its application and cosmetics | |
| CN107236054B (en) | A kind of preparation method and application of low molecular weight alveoli | |
| CN108410927B (en) | Method for producing high molecular weight hyaluronic acid by fermentation method | |
| WO2017113262A1 (en) | Antioxidative biocellulose and manufacturing method thereof | |
| CN112521430B (en) | Large-scale preparation method of sulfated kelp oligosaccharide suitable for cosmetics | |
| CN104745662A (en) | Method for preparing hyaluronic acid substitute through oxidizing chitosan by laccase-TEMPO system | |
| CN117024627A (en) | Preparation method and application of yeast nano beta-glucan dry powder | |
| CN117821544A (en) | Brown algae refining method | |
| CN105106075A (en) | Composition with nutritional and moisturizing efficacies as well as preparation method and application of composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |