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CN116172015B - Seed dressing agent for preventing and treating wheat stem-based rot, seed coating agent and comprehensive prevention and treatment method - Google Patents

Seed dressing agent for preventing and treating wheat stem-based rot, seed coating agent and comprehensive prevention and treatment method Download PDF

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Publication number
CN116172015B
CN116172015B CN202211356982.9A CN202211356982A CN116172015B CN 116172015 B CN116172015 B CN 116172015B CN 202211356982 A CN202211356982 A CN 202211356982A CN 116172015 B CN116172015 B CN 116172015B
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agent
wheat
seed dressing
seed
rot
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CN116172015A (en
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王祥会
焦玉霞
于玲雅
关秀敏
郭妍妍
成冰
曹增
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Zoucheng Agricultural And Rural Bureau
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Zoucheng Agricultural And Rural Bureau
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/06—Coating or dressing seed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having no bond to a nitrogen atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Soil Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
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  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a seed dressing agent and a seed coating agent for preventing and treating wheat stem basal rot and a comprehensive prevention and treatment method, belonging to the technical field of plant disease prevention and treatment. The compound seed dressing agent is prepared by compounding bacillus amyloliquefaciens, a mepiquat chloride aqueous solution and a thifluzamide agent, has a better control effect on stem-based rot caused by wheat infection caused by fusarium and the like, can be used for preventing and controlling plant diseases such as wheat stem-based rot caused by fusarium and the like, thereby better controlling the spread and epidemic of the diseases, ensuring the production safety of grains and slowing down the generation of plant disease resistance. Meanwhile, bactericides such as pyraclostrobin and the like are sprayed in the wheat green-returning period, so that the spread of diseases can be effectively restrained, joint defense is formed by the bactericide and the compound seed dressing agent, the comprehensive prevention and control effect is improved, a basis is provided for biological prevention and control of wheat stem-based rot in the future, and the application prospect is wide.

Description

Seed dressing agent for preventing and treating wheat stem-based rot, seed coating agent and comprehensive prevention and treatment method
Technical Field
The invention belongs to the technical field of plant disease control, and in particular relates to a seed dressing agent and a seed coating agent for controlling wheat stem basal rot and a comprehensive control method.
Background
Wheat stem rot is an important fungal soil-borne disease caused by fusarium infection, and spreads in a epidemic situation in a wheat producing area. After the wheat seeds are infected, the wheat seeds can be decomposed before germination; when the seedling stage is infected, brown disease spots appear on leaf sheaths and stems at the basal part of the wheat stems, a plurality of disease spots are combined together to cause browning and rot of the root parts of the wheat, and when the disease is serious, the wheat leaves are withered and died; when the infection of the later-stage diseases is serious, the disease can spread to the 6 th stem node; when the field environment is high in humidity, red or white mildew layers can be observed on the basal stem nodes, and finally, dry and white ears and small grain blighted ears are caused, and the yield of land parcels can be reduced by 20-70%, and serious land parcels even absolute yield can be caused. In recent years, because the quantity of fusarium in soil is increased greatly by popularizing straw and returning to the field in a large area, the wheat stem basal rot caused by the composite infection of a plurality of fusarium is in a trend of increasing year by year, and the safety production of wheat in China and even the world is seriously threatened.
At present, no variety for resisting the wheat stem basal rot is available, and although agricultural measures such as deep-ploughing, deep loosening, rotation, rapid straw decomposition and the like are adopted in the wheat planting process to show a certain control effect on the wheat stem basal rot, under the situation that the current straw burning is forbidden and the autumn is urgent, the straw returning is still the only effective form for rapidly processing the straw at present. At present, no chemical control agent specially registered for controlling the wheat stem basal rot is available, the difficulty of controlling the disease is also described, and from the combination of a biological agent and a chemical agent, the exploration of a control method for the wheat stem basal rot becomes necessary, and the prevention and control method is beneficial to slowing down the risk of increased drug resistance of the disease.
Disclosure of Invention
In the research of promoting prevention and treatment of wheat stem basal rot, the invention discovers that: the bacillus amyloliquefaciens can play a good role in inhibiting the wheat stem rot, and based on the bacillus amyloliquefaciens and other preparations, seed dressing is carried out in a single-agent or combined mode, and a bactericide is sprayed in the turning green period, so that the comprehensive control method of the wheat stem rot is explored. On the basis, the invention provides the following technical scheme:
the invention provides application of bacillus amyloliquefaciens in preparing a medicament with a wheat stem rot control effect.
In the above use, the agent is selected from seed dressing agents.
The invention provides a compound seed dressing agent which is prepared by compounding a bacillus amyloliquefaciens bacterial agent, a mepiquat chloride aqueous agent and a thifluzamide agent; wherein the bacterial content of the bacillus amyloliquefaciens bacterial agent is more than or equal to 1 multiplied by 10 10 CFU/mL, the concentration of the mepiquat chloride aqueous agent is 250g/L, and the concentration of the thifluzamide pharmaceutical agent is 240g/L.
The volume ratio of the bacillus amyloliquefaciens, the thifluzamide medicament and the mepiquat chloride aqua is selected from
1000:75-125:400; Preferably 1000:75:400 or 1000:125:400.
The bacillus amyloliquefaciens bacterial agent can be self-made or purchased commercially. The microbial inoculum is purchased from home or commercial sources, and the bacterial content in the microbial inoculum should meet the requirement of not less than 1X 10 10 CFU/mL. If the self-made mode is adopted to prepare the bacillus amyloliquefaciens bacterial agent, the technology disclosed in the patent CN201910812781.7 can be selected, and any other feasible prior art can be adopted, for example, the patent CN202011568910.1.
The mepiquat chloride aqueous solution can be self-made or purchased commercially. Homemade or commercially available, the concentration of mepiquat chloride in the aqueous formulation should be 250g/L.
The thifluzamide medicament can be self-made or purchased commercially. The thifluzamide concentration in the agent should be 240g/L, either self-made or commercially available. If the above thifluzamide agent is prepared in a self-made manner, it may be selected from the following: thifluzamide is dissolved in any pesticidally acceptable solvent (e.g., acetonitrile, toluene, methylene chloride, acetone, ethyl acetate, etc.) to prepare a thifluzamide pharmaceutical agent at a concentration of 240g/L.
In practical application, the compound seed dressing agent is selected from the following dosage:
1000-1750 mL of the compound seed dressing agent is selected for each 100kg of wheat seeds. Preferably, the dosages of the compound seed dressing agent are as follows: 1525mL of compound seed dressing agent is selected for each 100kg of wheat seeds; wherein, the volume ratio of the bacillus amyloliquefaciens agent to the thifluzamide agent to the mepiquat chloride aqueous solution is 1000:125:400.
The compound seed dressing agent has excellent inhibition effect on fusarium, so that the compound seed dressing agent can be applied to prevention and treatment of plant diseases caused by fusarium, including but not limited to wheat stem basal rot and the like. In addition, the invention also provides application of the compound seed dressing agent in preparation of a preparation for preventing and treating wheat stem basal rot.
The invention provides a novel seed coating agent, which contains the compound seed dressing agent.
The novel seed coating agent can also comprise a micro fertilizer acceptable for seed coating, a plant growth regulator, a film forming agent, an antifreezing agent, a substrate wetting agent and other auxiliary agents.
The invention provides a comprehensive control method for wheat stem basal rot, which specifically comprises the following steps: before sowing, the wheat is subjected to seed dressing or coating, and then a bactericide is sprayed in the wheat turning green period, so that the comprehensive control of the wheat stem basal rot is realized.
In the above-mentioned comprehensive control method, the medicine used for dressing seed is selected from the above-mentioned compound seed dressing medicine, and the seed coating agent used for coating is selected from the above-mentioned novel seed coating agent.
In the comprehensive control method, the bactericide is pyraclostrobin or propisochlor-azoxystrobin.
The beneficial effects of the invention are as follows:
the compound seed dressing agent has excellent inhibition effect on fusarium, can be used for preventing and controlling plant diseases caused by fusarium, such as wheat stem basal rot, and the like, thereby better controlling the spread and popularity of the diseases, ensuring the production safety of grains, and further slowing down the generation of plant disease and insect pest resistance. Meanwhile, bactericides such as pyraclostrobin and the like are sprayed in the wheat green-returning period, so that the spread of diseases can be effectively restrained, joint defense is formed by the bactericides and the compound seed dressing agent, the comprehensive prevention and control effect is improved, a basis is provided for biological prevention and control of the wheat stem basal rot in the future, and the application prospect is wide.
Detailed Description
The test materials and agents of the present invention are as follows:
Wheat variety: jimai 22, one of the local main cultivars, national trial wheat No. 2006018, is offered by Shandong Shengfeng seed company, inc. The components of the medicament are as follows: bacillus amyloliquefaciens (more than or equal to 100 hundred million CFU/mL), available from Wuhanke North Biotech Co., ltd; mepipinium aqua (250 g/L) (AS), qingdao Fengbang Agrochemical Co., ltd., shandong province; thifluzamide suspension (240 g/L) (SC), optical agrochemicals Co., ltd. Among the above agents, AS is an aqueous agent and SC is a suspending agent.
Other terms used in the present invention, unless otherwise indicated, generally have meanings commonly understood by those of ordinary skill in the art. The invention will be described in further detail below in connection with specific embodiments and with reference to the data. The following examples are intended to illustrate the invention and are not intended to limit the scope of the invention in any way.
Examples
1. Field test arrangement
The test was carried out in the village (35 deg. 33'N,116 deg. 88' E) of the northern Zhenzhen village of Shandong province, 11 months 12 days 2021, the land is flat, the soil fertility is uniform, the irrigation condition is good, and the top crop is corn. The base fertilizer is applied with 50kg of compound fertilizer with the ratio of P to K=15:15:15 per mu, the sowing quantity is 12.5kg/667m 2, the row spacing is 18cm during sowing, and the sowing depth is 3-5 cm.
2. Medicament seed dressing
Before sowing, the test agent was measured according to the dose in table 1, mixed with wheat, and seed-dressing was performed. Airing the seeds after seed dressing in a cool and ventilated place for standby sowing. The experiment was designed for a total of 8 groups, each group being repeated 3 times, 50m 2 per cell. The control group was: seed dressing is not performed.
TABLE 1 dosage of seed dressing agent per 100kg seed
Seedling stage investigation was carried out on day 25 of 12 in 2021, 10 seedlings were sampled at 3 points on diagonal line per cell, each treatment was repeated 3 times, a total of 30 seedlings were brought back to laboratory, disease conditions were recorded, and at this time seedlings could not be disease classified, and only disease rates were calculated. The calculation formula is as follows:
disease rate= (disease number/total number) x 100%.
The results of the investigation are shown as "rate of disease in seedling stage" in Table 2. As can be seen from table 2, the wheat stem basal rot is significantly controlled in the seedling stage, and the disease rate is significantly lower than that of the control group; especially group 7, exhibited very excellent control effect of wheat stem basal rot. In the prevention and treatment effect of seedling stage stem basal rot, the medicament compounding is superior to that of single use of each dose. Meanwhile, the bacillus amyloliquefaciens is slightly better than thifluzamide in the prevention and treatment effect of the wheat stem base rot, and the result has important value for developing new strains for preventing and treating the wheat stem base rot.
In the wheat turning green period (13 days of 3 months of 2022), the pesticide is sprayed on each cell by adopting 25% pyraclostrobin suspending agent according to the dosage of 40mL/667m 2, except for a control group.
The jointing stage investigation is carried out at the 3 rd month of 2022 and 25 th day, sampling is carried out according to seedling stage sampling, and jointing stage disease plant rate is calculated.
Spike phase investigation was performed at day 19, 5, 2022, to investigate Bai Sui rates and calculate disease indices. The sampling method of each district is the same as the seedling stage, 90 stems are taken out by each treatment three-point sampling method, the stems are brought back to a laboratory for classification, the classification standard is carried out by referring to the technical specification of stem basal rot detection and report of wheat in Shandong province, and the classification standard is as follows: level 0: the whole plant stalk is asymptomatic; stage 1: the leaf sheath 1 of the basal part of the overground part turns brown, but the stem node has no lesion; 3 stages: the 1 st stem node of the overground part turns brown; 5 stages: the 2 nd stem node of the overground part turns brown; 7 stages: brown lesions exceeded the 2 nd node, but were devoid of white ears; stage 9: brown lesions exceed the 2 nd stem node, producing white spikes or no spikes due to morbidity; calculating the disease index of the spike period. The calculation formula is as follows:
Disease rate= (disease number/total number) ×100%;
disease index=100×Σ (leaf number of each stage×representative value of each stage)/(total leaf number of investigation×representative value of highest stage).
The investigation results are shown as "rate of disease in jointing stage", "index of disease in spike stage", "yield" and "control effect" in table 2. As is clear from Table 2, the application of the composition in the returning period effectively suppresses the spread trend of the disease, and the disease rate is less variable than that of each treatment in the seedling stage. The disease rate of the control group is always in a higher proportion and always in an ascending state. The seed coating and the green returning period are subjected to pesticide spraying treatment, the investigation result of the prevention effect of the treatment 6 and the treatment 7 in the spike period exceeds 80 percent, the seed coating and the green returning period are in a more ideal state, the yield is higher than that of other treatments, and the yield is increased by more than 17 percent.
TABLE 2
The following is noted: the test data in Table 2 were obtained by performing analysis of variance on the investigation data of the wheat growth period by means of DPS (9.01) data processing system using the Duncan new complex polar difference method. The disease rates of the groups in table 2 do not completely show a consistent change rule, and the reason is mainly that the test field is large, sampling points in each period may not be in the same place, and the sampling test errors cause fluctuation of test data, but the fluctuation is in a reasonable range and does not influence the explanation of the technical effects of the invention.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the invention in any way, and any person skilled in the art may make modifications or alterations to the disclosed technical content to the equivalent embodiments. However, any simple modification, equivalent variation and variation of the above embodiments according to the technical substance of the present invention still fall within the protection scope of the technical solution of the present invention.

Claims (5)

1. The compound seed dressing medicament is characterized by being prepared by compounding a bacillus amyloliquefaciens bacterial agent, a mepiquat chloride aqueous agent and a thifluzamide medicament;
the bacterial content of the bacillus amyloliquefaciens bacterial agent is more than or equal to 1 multiplied by 10 10 CFU/mL, the concentration of the mepiquat chloride aqueous agent is 250g/L, and the concentration of the thifluzamide aqueous agent is 240g/L; the volume ratio of the bacillus amyloliquefaciens agent to the thifluzamide agent to the mepiquat chloride aqueous solution is selected from 1000:75-125:400.
2. The compound seed dressing agent of claim 1, wherein the dosage of the compound seed dressing agent is: 1525mL of compound seed dressing agent is selected for each 100 kg wheat seeds; wherein, the volume ratio of the bacillus amyloliquefaciens agent to the thifluzamide agent to the mepiquat chloride aqueous solution is 1000:125:400.
3. A novel seed coating agent, which is characterized in that the seed coating agent contains the compound seed dressing agent according to any one of claims 1-2.
4. A novel seed coating agent according to claim 3, wherein the seed coating agent further comprises a seed-acceptable micro-fertilizer, a plant growth regulator, a film forming agent, an antifreeze, a substrate wetting agent or other auxiliary agents.
5. A comprehensive control method for wheat stem rot is characterized by comprising the following specific steps: before sowing, seed dressing or coating is carried out on the wheat, and then a bactericide is sprayed in the wheat turning green period, so that the comprehensive control of the wheat stem basal rot is realized; wherein the medicament for seed dressing is the compound seed dressing medicament according to any one of claims 1-2; the seed coating agent used for coating is the novel seed coating agent of claim 3 or 4; the bactericide is pyraclostrobin or propiconazole azoxystrobin.
CN202211356982.9A 2022-11-01 2022-11-01 Seed dressing agent for preventing and treating wheat stem-based rot, seed coating agent and comprehensive prevention and treatment method Active CN116172015B (en)

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CN103109843A (en) * 2013-03-04 2013-05-22 中化蓝天集团有限公司 Insecticidal disease-preventing suspended seed coating containing thiamethoxam, thifluzamide and/or benzene kresoxim-methyl
CN110551666A (en) * 2019-10-09 2019-12-10 河南省农业科学院植物保护研究所 Bacillus amyloliquefaciens YB-130, microbial preparation and application thereof

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