CN116148459B

CN116148459B - Reading compensation method for chemiluminescent reader

Info

Publication number: CN116148459B
Application number: CN202310027942.8A
Authority: CN
Inventors: 倪剑锋; 吴骞
Original assignee: Zhejiang Baotai Intelligent Technology Co ltd
Current assignee: Zhejiang Baotai Intelligent Technology Co ltd
Priority date: 2023-01-09
Filing date: 2023-01-09
Publication date: 2024-02-23
Anticipated expiration: 2043-01-09
Also published as: CN116148459A

Abstract

The invention belongs to the field of in-vitro diagnostic instruments, and particularly relates to a reading compensation method for a chemiluminescent reading instrument, which comprises the following steps: step 1, establishing a compensation coefficient for each hole site in a chemiluminescent reader by taking a certain temperature as a reference value; and 2, correcting the signal value of each hole site by using a compensation coefficient when the chemiluminescent reader detects the signal value. According to the invention, by setting the correction coefficient for each hole, the detection accuracy of each hole is improved, and the reading repeatability of the chemiluminescent reader is further improved.

Description

Reading compensation method for chemiluminescent reader

Technical Field

The invention belongs to the field of in-vitro diagnostic instruments, and particularly relates to a reading compensation method for a chemiluminescent reader.

Background

When the 96-well chemiluminescent reader reads each well, the same measuring object is placed in each well, and the read signal values are inconsistent due to the temperature difference in each well, so that the read repeatability among 96 wells is affected, and the detection is not accurate enough.

Disclosure of Invention

In order to make up for the defects of the prior art, the invention provides a technical scheme of a reading compensation method for a chemiluminescent reader, which can reduce the problem of inconsistent reading signal values caused by the temperature difference of each hole and improve the detection repeatability.

A reading compensation method for a chemiluminescent reader, comprising:

step 1, establishing a compensation coefficient for each hole site in a chemiluminescent reader by taking a certain temperature as a reference value;

and 2, correcting the signal value of each hole site by using a compensation coefficient when the chemiluminescent reader detects the signal value.

Further, in the step 1, the compensation coefficient is: s=1+ (T _Datum -T _i ) N%, where S is the compensation coefficient, T _Datum Is a reference value of temperature, T _i The temperature value of the ith well is given, and N is the temperature increase value.

Further, the T is _Datum 30-40.

Further, the N is 3-10.

Further, in the step 2, the detected signal value is multiplied by a compensation coefficient to obtain a modified signal value.

Compared with the prior art, the invention has the beneficial effects that:

according to the invention, by setting the correction coefficient for each hole, the detection accuracy of each hole is improved, and the reading repeatability of the chemiluminescent reader is further improved.

Drawings

FIG. 1 is a flow chart of the present invention.

Detailed Description

In the description of the present invention, it should be understood that the terms "one end," "the other end," "the outer side," "the upper," "the inner side," "the horizontal," "coaxial," "the center," "the end," "the length," "the outer end," and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, merely to facilitate description of the present invention and simplify the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention.

The invention is further described below with reference to the accompanying drawings.

Referring to fig. 1, a reading compensation method for a chemiluminescent reader includes:

and 1, establishing a compensation coefficient for each hole site in the chemiluminescent reader by taking a certain temperature as a reference value.

Wherein, compensation coefficient is: s=1+ (T _Datum -T _i ) N%, S is compensation coefficient, T _Datum For the reference value of the temperature, in the range of 30-40, preferably 37, T _i For the temperature value of the ith well, N is a temperature increase value in the range of 3 to 10, preferably 5.

And 2, when the chemiluminescent reader detects the signal value, correcting the signal value of each hole site by using a compensation coefficient, specifically, multiplying the detected signal value by the compensation coefficient to obtain a corrected signal value. The above calculation is performed by a computer.

Examples

The invention is applied to a chemiluminescent immunoassay analyzer in the in-vitro diagnosis industry, taking a 96-hole reader (hole sites are generally 8 rows and 12 columns or 8 columns and 12 rows) as an example, the temperatures of different hole sites are different and generally show that the middle temperature is high and the surrounding temperature is low, and the temperature is related to a temperature gradient effect, the invention obtains the temperature in each hole through software theoretical simulation and actual measurement, and establishes the following temperature map:

	1#	2#	3#	4#	5#	6#	7#	8#	9#	10#	11#	12#
													A	36.50	36.54	36.58	36.62	36.62	36.62	36.62	36.62	36.62	36.58	36.52	36.40
B	36.80	36.84	36.88	36.92	36.92	36.92	36.92	36.92	36.92	36.88	36.82	36.70
													C	37.00	37.04	37.08	37.12	37.12	37.12	37.12	37.12	37.12	37.08	37.02	36.90
D	37.10	37.14	37.18	37.22	37.22	37.22	37.22	37.22	37.22	37.18	37.12	37.00
													E	37.10	37.14	37.18	37.22	37.22	37.22	37.22	37.22	37.22	37.18	37.12	37.00
F	37.10	37.14	37.18	37.22	37.22	37.22	37.22	37.22	37.22	37.18	37.12	37.00
													G	36.90	36.94	36.98	37.02	37.02	37.02	37.02	37.02	37.02	36.98	36.92	36.80
H	36.60	36.64	36.68	36.72	36.72	36.72	36.72	36.72	36.72	36.68	36.62	36.50

TABLE 1

Then using the formula 1+ (37-T) with 37 ℃ as a reference _i ) Correction was performed for each well 5%, T _i For the temperature value of each hole, correcting upwards when the temperature is lower than 37 ℃, and correcting downwards when the temperature is higher than 37 ℃, so as to obtain the correction coefficient of each hole:

	1#	2#	3#	4#	5#	6#	7#	8#	9#	10#	11#	12#
													A	1.025	1.023	1.021	1.019	1.019	1.019	1.019	1.01g	1.019	1.021	1.024	1.030
B	1.010	1.008	1.006	1.004	1.004	1.004	1.004	1.004	1.004	1.006	1.009	1.015
													C	1.000	0.998	0.996	0.994	0.994	0.994	0.994	0.994	0.994	0.996	0.999	1.005
D	0.995	0.993	0.991	0.989	0.989	0.989	0.989	0.989	0.989	0.991	0.994	1.000
													E	0.995	0.993	0.991	0.989	0.989	0.989	0.989	0.989	0.989	0.991	0.994	1.000
F	0.995	0.993	0.991	0.989	0.989	0.989	0.989	0.989	0.989	0.991	0.994	1.000
													G	1.005	1.003	1.001	0.999	0.999	0.999	0.999	0.999	0.999	1.001	1.004	1.010
H	1.020	1.018	1.016	1.014	1.014	1.014	1.014	1.014	1.014	1.016	1.019	1.025

TABLE 2

Correcting the detection signal value of each hole according to the modification coefficient, wherein the signal value data before correction is as follows:

	1#	2#	3#	4#	5#	6#	7#	8#	9#	10#	11#	12#
													A	61509	60271	61450	61469	61455	61587	61504	61870	61465	62539	61445	61094
B	62528	61991	62554	63178	62581	62830	62531	62870	62574	64086	62561	60482
													C	63245	62789	63224	63301	63222	63803	63316	63916	63273	63811	63255	62012
D	63372	62580	63286	63874	63283	64206	63318	63738	63354	63796	63313	61875
													E	63316	62931	63303	63325	63331	64263	63391	64083	63325	64134	63313	61293
F	63244	63158	63220	63195	63271	63625	63229	64381	63285	63309	63243	61826
													G	63178	62875	63234	63346	63221	63298	63197	64104	63209	63513	63163	62056
H	62241	62473	62273	61590	62175	63429	62183	63263	62217	61526	62193	60977

TABLE 3 Table 3

Wherein mean=62828, sd=884, cv=1.4%.

The corrected signal value data are:

	1#	2#	3#	4#	5#	6#	7#	8#	9#	10#	11#	12#
													A	63046	61657	62740	62636	62622	62757	62672	63045	62632	63852	62919	62926
B	63153	62486	62929	63430	62831	63081	62781	63121	62824	64470	63124	61389
													C	63245	62663	62971	62921	62842	63420	62936	63532	62893	63555	63191	62322
D	63055	62141	62716	63171	62586	63499	62621	63036	62657	63221	62933	61875
													E	62999	62490	62733	62628	62634	63556	62693	63378	62628	63556	62933	61293
F	62927	62715	62651	62499	62575	62925	62533	63672	62588	62739	62863	61826
													G	63493	63063	63297	63282	63157	63234	63133	64039	63145	63576	63415	62676
H	63485	63597	63269	62452	63045	64317	63053	64148	63088	62510	63374	62501

TABLE 4 Table 4

Wherein mean=62953, sd=534, cv=0.8%.

CV represents repeatability, SD represents standard deviation of 96-well signal values, MEAN represents average value of 96-well signal values, and smaller repeatability cv=sd/MEAN represents better repeatability.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.

Claims

1. A reading compensation method for a chemiluminescent reader, comprising:

step 1, taking a certain temperature as a reference value to build each hole site in a chemiluminescent readerAnd (3) standing a compensation coefficient, wherein the compensation coefficient is as follows: s=1+ (T _Datum -T _i ) N%, where S is the compensation coefficient, T _Datum Is a reference value of temperature, T _i The temperature value of the ith hole is N, which is a temperature increasing value and N is 5;

and 2, when the chemiluminescent reader detects the signal value, correcting the signal value of each hole site by using a compensation coefficient, specifically, multiplying the detected signal value by the compensation coefficient to obtain a corrected signal value.

2. The method for reading compensation of chemiluminescent reader of claim 1 wherein the T is _Datum 30-40.