CN116139178B

CN116139178B - Livestock dry powder with disinfection function and preparation method thereof

Info

Publication number: CN116139178B
Application number: CN202310141930.8A
Authority: CN
Inventors: 郑建龙; 艾琴; 农意承; 邹增丁; 方炳虎
Original assignee: Guangdong Wens Dahuanong Biotechnology Co ltd
Current assignee: Guangdong Wens Dahuanong Biotechnology Co ltd
Priority date: 2023-02-21
Filing date: 2023-02-21
Publication date: 2024-04-26
Anticipated expiration: 2043-02-21
Also published as: CN116139178A

Abstract

The invention provides livestock dry powder with a disinfection function and a preparation method thereof, and belongs to the technical field of veterinary disinfectants. The dry powder for livestock comprises, by weight, 50% -85% of montmorillonite, 5% -30% of sepiolite, 5% -10% of silicon dioxide, 2% -10% of plant ash, 0.01% -0.1% of thymol, 0.1% -0.5% of chloramine-T and 0.02% -0.1% of ammonium chloride. The invention can not only rapidly dry the surfaces of piglets and wet environment, but also has broad-spectrum antibacterial and antivirus capabilities, has aromatic and not-pungent taste, can promote the survival rate of piglets, repel mosquitoes and flies, control the breeding of bacteria and viruses in the environment and block the transmission of diseases through the collocation of montmorillonite, sepiolite, silicon dioxide, plant ash, thyme atmosphere, chloramine T and ammonium chloride.

Description

Livestock dry powder with disinfection function and preparation method thereof

Technical Field

The invention belongs to the technical field of veterinary disinfectants, and particularly relates to livestock dry powder with a disinfection function and a preparation method thereof.

Background

With the increasing intensive production degree of pig raising, disinfection work becomes one of important links in pig raising production, and in winter and spring, the disinfection work is a high-onset period of pig raising diseases, especially diarrhea, swine fever and other diseases, and serious economic loss is brought to pig farms. When the pig farm is ill, how to disinfect pigs becomes a difficult problem. At present, liquid disinfectants, quicklime and the like are often used as disinfectants in pig farms, but the following disadvantages exist: (1) The use of the liquid disinfectant is easy to increase the humidity of the housing, is not beneficial to the use of the livestock in the closing of the housing in winter and spring, and is not suitable for the disinfection of diarrhea piglets and sick and weak pigs; (2) The quick lime is used as a disinfectant, so that the pig feed additive has irritation to pigs and operators.

Therefore, aiming at the problems, it is necessary to develop a dry powder with antibacterial and disinfecting functions and moisture absorption and drying functions, which is used in winter and spring under inconvenient use of liquid disinfectants or in a humid environment or for drying and disinfecting the body surfaces of pigs, so that the disinfection irritation of personnel and animals is reduced.

Disclosure of Invention

In order to solve the technical problems, the invention provides livestock dry powder with a disinfection function and a preparation method thereof.

In order to achieve the above purpose, the present invention proposes the following technical solutions:

One of the technical proposal is as follows: the dry livestock powder with the disinfection function comprises, by weight, 50% -85% of montmorillonite, 5% -30% of sepiolite, 5% -10% of silicon dioxide, 2% -10% of plant ash, 0.01% -0.1% of thymol, 0.1% -0.5% of chloramine-T and 0.02% -0.1% of ammonium chloride.

Further, the raw materials comprise 59.87% of montmorillonite, 25% of sepiolite, 10% of silicon dioxide, 5% of plant ash, 0.01% of thymol, 0.1% of chloramine-T and 0.02% of ammonium chloride by weight.

The second technical scheme is as follows: a preparation method of livestock dry powder with a disinfection function comprises the following steps:

(1) Drying montmorillonite, sepiolite, silicon dioxide and plant ash for standby;

(2) Weighing the raw materials according to the weight percentage, mixing, crushing and sieving the silicon dioxide, plant ash, thymol, chloramine-T and ammonium chloride to obtain a mixture A;

(3) And adding the mixture A into the dried montmorillonite and sepiolite, and uniformly mixing and stirring to obtain the livestock dry powder with the disinfection function.

Further, in the step (1), the drying temperature is 100-105 ℃ and the drying time is 2 hours.

Further, in the step (2), the mixture is crushed and sieved by a 80-mesh sieve.

Further, in the step (3), the time of mixing and stirring is 15min, and the stirring speed is 10r/min.

Compared with the prior art, the invention has the following advantages and technical effects:

(1) The sepiolite selected by the invention has a porous structure and a large specific surface area, has strong adsorptivity, is matched with montmorillonite and silicon dioxide, can rapidly dry the body surface of newborn piglets, reduces the body surface temperature loss of the newborn piglets, improves the activity of the piglets, and improves the survival rate of the piglets; meanwhile, the wet culture environment can be quickly dried, and the survival probability of bacteria and viruses in the environment is reduced.

(2) The chloramine T selected by the invention is a broad-spectrum chlorine-containing disinfectant, has the effect of killing bacteria, viruses, fungi and spores, and has small irritation compared with common chlorine-containing disinfectants such as sodium trichloroisocyanurate; meanwhile, the disinfection effect can be improved by matching with ammonium chloride.

(3) The plant ash selected by the invention can accelerate the dry powder agent to fall off from the body surface in the body surface drying process of the piglets.

(4) The thymol selected by the invention has aromatic smell, can repel mosquitoes and flies, avoid animals from being bitten by the mosquitoes and flies, and can reduce the pungent smell of chloramine T.

Therefore, in conclusion, the piglet feed additive disclosed by the invention can be used for rapidly drying the body surface of a piglet and a humid environment through the collocation of montmorillonite, sepiolite, silicon dioxide, plant ash, thyme atmosphere, chloramine T and ammonium chloride, has broad-spectrum antibacterial and antivirus capabilities, is fragrant and not harsh in taste, can be used for improving the survival rate of the piglet, expelling mosquitoes and flies, controlling the breeding of bacteria and viruses in the environment and blocking the transmission of diseases.

Drawings

The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the application. In the drawings:

FIG. 1 is a graph showing the results of the water absorption test of the dry livestock powder prepared in example 1;

FIG. 2 is a graph showing the results of the water absorption test of the dry livestock powder prepared in example 2;

FIG. 3 is a graph showing the results of the water absorption test of the dry livestock powder prepared in example 3;

FIG. 4 is a graph showing the results of the water absorption test of the dry livestock powder prepared in example 4;

FIG. 5 is a graph showing the results of the water absorption test of the dry livestock powder prepared in example 5;

FIG. 6 is a graph showing the results of the water absorption test of the dry livestock powder prepared in comparative example 1;

FIG. 7 is a graph showing the results of the water absorption test of the dry livestock powder agent prepared in comparative example 2.

Detailed Description

Various exemplary embodiments of the invention will now be described in detail, which should not be considered as limiting the invention, but rather as more detailed descriptions of certain aspects, features and embodiments of the invention.

It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In addition, for numerical ranges in this disclosure, it is understood that each intermediate value between the upper and lower limits of the ranges is also specifically disclosed. Every smaller range between any stated value or stated range, and any other stated value or intermediate value within the stated range, is also encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.

It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the invention described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from consideration of the specification of the present invention. The specification and examples of the present invention are exemplary only.

As used herein, the terms "comprising," "including," "having," "containing," and the like are intended to be inclusive and mean an inclusion, but not limited to.

The invention provides livestock dry powder with a disinfection function, which comprises, by weight, 50% -85% of montmorillonite, 5% -30% of sepiolite, 5% -10% of silicon dioxide, 2% -10% of plant ash, 0.01% -0.1% of thymol, 0.1% -0.5% of chloramine-T and 0.02% -0.1% of ammonium chloride.

In some preferred embodiments of the present invention, the raw materials comprise 59.87% montmorillonite, 25% sepiolite, 10% silica, 5% plant ash, 0.01% thymol, 0.1% chloramine-T and 0.02% ammonium chloride by weight.

The invention also provides a preparation method of the livestock dry powder with the disinfection function, which comprises the following steps:

The montmorillonite, sepiolite, silicon dioxide and plant ash have the main functions of absorbing moisture in piglets and the environment through the pore structure of the montmorillonite, sepiolite, silicon dioxide and plant ash, so that the aim of drying is fulfilled. In principle, the drying temperature and time are preferably such that the weight of montmorillonite, sepiolite, silica and plant ash is reduced to a constant value.

Sepiolite is a magnesium-rich silicate clay mineral. The theoretical chemical formula is Mg ₈[Si₂O₃₀](OH)₄·12H₂ O, and 4 water molecules are crystal water: the balance of zeolite water. The sepiolite is a chain structure of hydrous aluminum magnesium silicate mineral. The chain structure also contains small units with lamellar structure, which is 2: layer 1. The cell layer pores of sepiolite can be widened toMaximum reachable/>More water molecules (zeolite water) can be contained, so that the sepiolite has excellent physical and chemical properties and technological properties, and meanwhile, the fine chains are pulled together due to the three-dimensional bond structure and Si-O-Si bonds, so that the sepiolite has a special crystal form which is prolonged consistently, and therefore, the sepiolite particles are rod-shaped and the fine particles are fibrous. The open groove pivot in the structure is parallel to the long axis of the crystal, so that the absorption capacity of the groove pivot is extremely strong. Sepiolite is relatively stable in properties at low temperatures, and the drying process is mainly aimed at removing zeolite water in pores, but when the temperature is too high, the zeolite water is lost, and the structure of the sepiolite is folded along with heating water loss, namely tetrahedral sheets are bent at turning parts, and the volume of channels is reduced, so that the adsorptivity of the sepiolite is reduced. Therefore, the temperature should not be so high during the drying process that the zeolite water in the sepiolite is removed, but the crystal water is preferably retained.

Based on the above considerations, in some preferred embodiments of the present invention, in step (1), the drying is performed at a temperature of 100-105 ℃ for a period of 2 hours.

The purpose of pulverization is to increase the specific surface area of the raw material, thereby improving the adsorption. In principle, the present invention is not particularly limited in terms of the pulverized particle size, but in some preferred embodiments of the present invention, in step (2), the pulverization is passed through an 80-mesh sieve, based on the consideration of increasing the specific surface area and energy saving.

The mixing and stirring function is to mix the raw materials sufficiently so that they combine with each other to act synergistically. In principle, the time and the rotation speed of the mixing and stirring are not limited, so long as the purpose of sufficiently mixing the raw materials can be achieved, but in some preferred embodiments of the present invention, in step (3), the time of the mixing and stirring is 15min, and the stirring rotation speed is 10r/min, based on the energy saving consideration.

All the raw materials used in the embodiment of the invention are commercially available.

The technical scheme of the invention is further described by the following examples.

Example 1

(1) Drying montmorillonite, sepiolite, silicon dioxide and plant ash at 105 ℃ for 2 hours for later use;

(2) Weighing 59.87% of montmorillonite, 25% of sepiolite, 10% of silicon dioxide, 5% of plant ash, 0.01% of thymol, 0.1% of chloramine-T and 0.02% of ammonium chloride according to weight percentage, mixing and crushing the silicon dioxide, the plant ash, the thymol, the chloramine-T and the ammonium chloride, and sieving the mixture through a 80-mesh sieve to obtain a mixture A;

(3) And (3) adding the mixture A into the montmorillonite and sepiolite dried in the step (1), and mixing and stirring for 15min at the speed of 10r/min to obtain the livestock dry powder with the disinfection function.

Example 2

(2) Weighing 70.84% of montmorillonite, 15% of sepiolite, 8% of silicon dioxide, 5.75% of plant ash, 0.05% of thymol, 0.3% of chloramine-T and 0.06% of ammonium chloride according to weight percentage, mixing and crushing the silicon dioxide, the plant ash, the thymol, the chloramine-T and the ammonium chloride, and sieving the mixture through a 80-mesh sieve to obtain a mixture A;

Example 3

(2) Weighing 81.4% of montmorillonite, 10% of sepiolite, 5% of silicon dioxide, 2.9% of plant ash, 0.1% of thymol, 0.5% of chloramine-T and 0.1% of ammonium chloride according to weight percentage, mixing and crushing the silicon dioxide, the plant ash, the thymol, the chloramine-T and the ammonium chloride, and sieving the mixture through a 80-mesh sieve to obtain a mixture A;

Example 4

(1) Drying montmorillonite, sepiolite, silicon dioxide and plant ash at 100deg.C for 2 hr;

(2) Weighing 50% of montmorillonite, 30% of sepiolite, 9.39% of silicon dioxide, 10% of plant ash, 0.01% of thymol, 0.5% of chloramine-T and 0.1% of ammonium chloride according to weight percentage, mixing and crushing the silicon dioxide, the plant ash, the thymol, the chloramine-T and the ammonium chloride, and sieving the mixture through a 80-mesh sieve to obtain a mixture A;

Example 5

(2) Weighing 85% of montmorillonite, 5% of sepiolite, 7.685% of silicon dioxide, 2% of plant ash, 0.075% of thymol, 0.2% of chloramine-T and 0.04% of ammonium chloride according to weight percentage, mixing and crushing the silicon dioxide, the plant ash, the thymol, the chloramine-T and the ammonium chloride, and sieving with an 80-mesh sieve to obtain a mixture A;

Comparative example 1

(2) Weighing 81.4% of montmorillonite, 10% of sepiolite, 5.1% of silicon dioxide, 2.9% of plant ash, 0.1% of thymol and 0.5% of chloramine-T according to weight percentage, mixing and crushing the silicon dioxide, the plant ash, the thymol and the chloramine-T, and sieving with a 80-mesh sieve to obtain a mixture A;

Comparative example 2

(2) Weighing 81.4% of montmorillonite, 10% of sepiolite, 5.5% of silicon dioxide, 2.9% of plant ash, 0.1% of thymol and 0.1% of ammonium chloride according to weight percentage, mixing and crushing the silicon dioxide, the plant ash, the thymol and the ammonium chloride, and sieving with a 80-mesh sieve to obtain a mixture A;

Performance testing

Experimental example 1 Dry powder Water absorption measurement

A funnel with a diameter of 9cm (glass funnel commonly used in laboratories) was selected, and after folding a filter paper with a diameter of 18cm, it was placed against the wall of the funnel, and the funnel was placed on a 50mL glass measuring cylinder. Weighing 10g of the dry powder agent in the examples 1-3 respectively, pouring the dry powder agent into a funnel paved with filter paper correspondingly, adding 30mL of double distilled water to the dry powder agent respectively, allowing water flow to slowly pass through the dry powder agent and the filter paper for 20min, and ensuring that no water drops drop out of the filter paper after the water is completely added for 3min continuously; meanwhile, similar products on the market are used as a control group, the control group 1 is an Anhui area collection product (the main components are modified aluminosilicate, mineral adsorbents, plant adsorbents and plant essential oils), the control group 2 is a Hunan area collection product (the main components are mineral adsorbents, plant adsorbents and plant essences), and the measurement process is the same as that of the example. The water absorption of the dry powder was calculated by recording the water absorption after filtration, and the water absorption test results of examples 1 to 5 and comparative examples 1 to 2 are shown in FIGS. 1 to 7, respectively, and the results are shown in Table 1.

TABLE 1 determination of the Dry powder Water absorption

As can be seen from Table 1, the dry powder agent prepared in the example of the present invention has a water absorption capacity superior to that of the like products on the market, and has excellent drying capacity.

Experimental example 2 inhibition effect of Dry powder on bacteria

The measurement is carried out by adopting co-culture, the clinically separated escherichia coli, staphylococcus aureus and salmonella are cultured to prepare bacterial liquid with the concentration of 1 multiplied by 10 ⁸ CFU/mL, then 10g of dry powder samples prepared in examples 1-5 and comparative examples 1-2 are respectively weighed, added into 10mL of bacterial liquid (with the concentration of 1 multiplied by 10 ⁸ CFU/mL), uniformly stirred, and then the mixture is waited for 15 minutes at room temperature, and the number of viable bacteria is detected by a gradient dilution plating method, and the result is shown in Table 2.

TABLE 2 inhibition of bacteria by dry powder

As can be seen from the data in Table 2, the dry powders of examples 1-5 have potent inhibitory effects on clinically isolated E.coli, staphylococcus aureus, salmonella; comparative example 1 in the absence of ammonium chloride and comparative example 2 in the absence of ammonium chloride-T, the bacteriostatic effect was reduced.

Experimental example 3 in vitro killing effect of dry powder on pseudorabies, porcine epidemic diarrhea and porcine rotavirus

1G of each of the dry powders of examples 1 to 5 and comparative examples 1 to 2 was weighed out, and the dry powders were purified according to the formula 1:10, sucking 800 mu L of dry powder diluent, respectively reacting with 100 mu L of pseudorabies virus liquid, porcine epidemic diarrhea virus liquid and porcine rotavirus liquid for 5min, and then adding 100 mu L of neutralizer (4% sodium thiosulfate physiological saline) for termination reaction. And (3) sucking the supernatant of the mixed solution, extracting the viral nucleic acid, and performing fluorescent quantitative PCR to detect the corresponding viral nucleic acid. A blank was also set up and the results are shown in tables 3-5.

TABLE 3 results of killing pseudorabies virus by dry powder

Table 4 results of the dry powder formulations for killing porcine epidemic diarrhea virus

Table 5 results of the dry powder formulation against porcine rotavirus

As can be seen from the data in tables 3-5, the dry powder of examples 1-5 was not detected with the corresponding viral nucleic acid Ct value after 15min of the respective actions with pseudorabies virus solution, porcine epidemic diarrhea virus solution, porcine rotavirus solution, indicating that it has a destructive effect on viral nucleic acid, the viral nucleic acid was destroyed, and the corresponding virus had been inactivated.

Experimental example 4 Effect of dry powder on the production Performance of farrowing house piglets

20 Sows to be produced (in a delivery house) are selected and divided into a control group and a test group, wherein 10 sows in each group are sprayed on a production bed and an incubator by using the dry powder prepared in the embodiment 1 of the invention, the dosage is 100g/m ³ twice a week, meanwhile, the newborn piglets are subjected to delivery disinfection by using the dry powder prepared in the embodiment 1, the control group is subjected to no dry powder disinfectant spraying treatment by using the obstetric bed and incubator, meanwhile, the newborn piglets are subjected to treatment by using a traditional method of wiping clean towels, the rest of feeding management is the same as that of the test group, the treatment is completed until weaning of the piglets, and the diarrhea rate and the survival rate of the piglets in the test period are counted, and the results are shown in table 6.

TABLE 6 influence of dry powder on the productivity of piglets in a farrowing house

Group of	Sow number of head	Piglet head number	Number of diarrhea	Number of dead head	Diarrhea rate/%	Survival rate/%
							Test group	10	126	8	6	6.35	95.2
Control group	10	121	32	10	26.44	91.7

As can be seen from Table 6, the test group using the dry powder agent prepared in example 1 of the present invention had a higher survival rate of piglets than the control group and a lower diarrhea rate than the control group.

The present application is not limited to the above-mentioned embodiments, and any changes or substitutions that can be easily understood by those skilled in the art within the technical scope of the present application are intended to be included in the scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. The livestock dry powder with the disinfection function is characterized in that the raw materials comprise 50-85% of montmorillonite, 5-30% of sepiolite, 5-10% of silicon dioxide, 2-10% of plant ash, 0.01-0.1% of thymol, 0.1-0.5% of chloramine-T and 0.02-0.1% of ammonium chloride according to weight percentage;

The preparation method of the livestock dry powder with the disinfection function comprises the following steps:

(3) Adding the mixture A into the dried montmorillonite and sepiolite, and uniformly mixing and stirring to obtain the livestock dry powder with the disinfection function;

In the step (1), the drying temperature is 100-105 ℃ and the drying time is 2h.

2. The dry livestock powder with disinfection function according to claim 1, wherein the raw materials consist of 59.87% by weight of montmorillonite, 25% by weight of sepiolite, 10% by weight of silica, 5% by weight of plant ash, 0.01% by weight of thymol, 0.1% by weight of chloramine-T and 0.02% by weight of ammonium chloride.

3. The dry livestock powder with a sterilizing function according to claim 1, wherein in the step (2), the powder is crushed and passed through a 80-mesh sieve.

4. The dry livestock powder with a sterilizing function according to claim 1, wherein in the step (3), the mixing and stirring time is 15min and the stirring speed is 10r/min.