CN116098840A

CN116098840A - Use of white truffle extract or composition containing same

Info

Publication number: CN116098840A
Application number: CN202211397332.9A
Authority: CN
Inventors: 李善珠; 金炳贤
Original assignee: LG Household and Health Care Ltd
Current assignee: LG H&H Co Ltd
Priority date: 2021-11-11
Filing date: 2022-11-09
Publication date: 2023-05-12
Also published as: KR20230068603A

Abstract

The present invention provides a use of a white truffle extract or a composition comprising the same, which relates to a composition for improving skin, comprising white truffle (Tuber Magnatum) as an active ingredient, and more particularly, to a cosmetic, skin external preparation composition and pharmaceutical external preparation composition for improving skin barrier, improving skin texture, improving skin moisture and improving atopic dermatitis, comprising white truffle extract as an active ingredient. The white truffle extract according to the present invention improves skin texture by promoting the activity of skin stem cells, strengthens skin barrier by promoting PPARr expression and/or filaggrin expression, and improves skin moisturizing ability, thereby also having an effect of preventing or treating atopic dermatitis, and thus can be effectively used for cosmetics, foods, pharmaceuticals or pharmaceutical external products for improving skin health and treating skin diseases.

Description

Use of white truffle extract or composition containing same

Technical Field

The present invention relates to a composition for improving skin comprising white truffle (white Magnatum) as an active ingredient, and more particularly, to a cosmetic, food, pharmaceutical external product and pharmaceutical composition for improving skin barrier, improving skin texture, improving skin moisture and improving atopic dermatitis, comprising white truffle extract as an active ingredient.

Background

The epidermis (epidermis), which is the outermost layer of the skin, has a protective function against various physical, chemical and mechanical stimuli from the outside, preventing excessive dissipation of body moisture through the skin. This protective function can be achieved by normal formation and maintenance of the stratum corneum, which is made up of keratinocytes. Keratinocytes are cells formed by stepwise morphological and functional changes while basal cells (basal cells) which proliferate continuously move to the stratum corneum (stratum corneum), and after a certain period of time, old keratinocytes are detached from the skin, and new keratinocytes rising from the lowest layer of the epidermis repeat the process of epidermal differentiation (epidermis differentiation) or keratinization (keratinization) replacing their functions. During this keratinization process, keratinocytes produce natural moisturizing factors (Natural Moisturizing Factor; NMF) and intercellular lipids such as ceramides, cholesterol and fatty acids, which act as a barrier to the outside of the stratum corneum, thus functioning as a skin barrier.

The skin texture, which is the skin surface morphology, is characterized by a three-dimensional microstructure formed of fine lines, and varies significantly from one age to another and from one location to another. Skin texture is divided into 1 (about 20-100 μm), 2 (about 5-40 μm), 3 (about 0.5 μm) lines (lines) according to the depth of the lines, and has the following features: the better the skin is, the more obvious the mesh-like network structure is formed by each line, and the polygons and stars (star formation) formed by intersecting lines are (non-patent document 1).

However, it is known that as the age increases or as the skin is damaged, the network structure of the fine lines which are originally dense collapses, the fine lines (2 times, 3 times, etc.) disappear, and 1 time of lines become deeper, and wrinkles are generated (non-patent documents 2, 3). That is, the representative symptom that occurs with skin aging, namely, wrinkles, has been the accumulation of minute changes that occur over a long period of time.

It is known that, embryologically, all the constituent components of human skin are derived from ectoderm or mesoderm. Epidermis, hair follicle, sebaceous glands, sweat glands, etc. originate from ectoderm, melanocytes, nerves and specific sensory receptors originate from neuroectoderm. According to embryological stages, stem cells of embryos are repeatedly differentiated into characteristic cells conforming to respective tissue functions, a certain number of stem cells remain in tissues even after becoming adult, and stem cells are mainly present in two places in the skin. The first is found in hair follicles (hair follicles). It is known that cells before cell differentiation are important for regeneration of epidermis and also for regeneration and growth of hair. The second is the basal layer (the basal layer of epidermis) of the epidermis. It has been shown that stem cells located here take care of not only the epidermis but also the fibroblasts of the dermis layer, thus playing an important role in protecting skin health. The stem cells herein have the advantage of being relatively large in quantity and readily available, and are therefore widely used in skin stem cell research. The skin is continuously regenerated (rejuvenated), and epidermal stem cells (epidermal stem cells) which are skin stem cells present in the epidermis of the skin are involved in the restoration of the epidermis after a wound (non-patent document 4).

In addition, simply increasing the size or number of epidermal cells, keratinocytes or fibroblasts does not solve the problem in regenerating normal skin or improving skin texture. Only when the epidermal stem cells of the epidermis are activated, the skin barrier is restored to its original state and to its normal, smooth state (non-patent document 5). The skin stem cells are also called skin stem cells (skin stem cells), and when the skin stem cells are activated, skin wounds such as trauma and the like can be treated or wound treatment can be promoted, and it is also expected that wrinkles of the skin can be relieved. It has been reported that, as an index of skin stem cells, the maintenance of skin stem cells required for epidermal morphogenesis and differentiation is regulated by p63 protein using integrin β1 (integrin β1) and integrin α6 (integrin α6).

Skin stem cells play an important role in maintaining the health and physiological and biochemical constancy of the skin. The stem cells present in the skin are also functionally abnormal due to the influence of aging, whereby the constancy of the skin is broken, and various problems occur. Therefore, various phenomena of skin aging can be improved by the activity of such stem cells (non-patent document 6).

In addition, there is a strong demand for the development of a component having activity of regenerating skin, improving characteristics, moisturizing skin and improving skin texture, which is safe for living organisms, stable in active ingredient, and more excellent in effect than the conventional one having effects of regenerating skin, improving characteristics (atopy), moisturizing skin and improving skin texture.

One of the causes of roughening skin texture is the phenomenon that the stem cell properties of skin stem cells existing in the skin decrease with age. The decrease in stem cell properties of stem cells present in the basal layer of the epidermis rapidly causes deterioration of skin barrier and regeneration ability, etc., and causes abnormal skin thickness, which makes the skin texture rough. However, the reality is that skin care products aimed at this mechanism are under-developed.

Currently existing techniques for regenerating skin and improving skin texture mainly focus on keratinocytes or fibroblasts, which are techniques for differentiating cells to some extent, and thus it is actually difficult to expect radical changes. The problem is that in many cases, rather than being the result obtained by changing the actual cells, it is rather by means of moisturizing.

Since skin stem cells are cells present in the skin and capable of differentiating into various cells, it is expected that the above-described problems can be more fundamentally solved by a method capable of more fundamentally improving the skin.

Prior art literature

Non-patent literature

(non-patent document 1) Journal of the European Academy of Dermatology and Venereigy, 12:103-114,1999.

(non-patent document 2) The British journal of dematology, 110:129-138,1984.

(non-patent document 3) Skin research and technology.5:189-194,1999.

(non-patent document 4) Annu.Rev.Cell Dev.biol.,22:339-373,2006.

(non-patent document 5) J Clin invest, 113 (3): 390-400,2004.

(non-patent document 6) Stem Cell Aging and Regenerative medicine, 43:986-997,2008.

Disclosure of Invention

Problems to be solved

As a result of the intensive studies to achieve the above object, the inventors of the present invention have confirmed that a white truffle extract promotes skin regeneration by promoting proliferation of skin stem cells, and that PPARr has excellent activity, has an effect of improving characteristics, shows an effect of improving skin moisturization due to an increase in expression of filaggrin, and shows an effect of improving skin texture, and also shows an effect of promoting proliferation of skin stem cells and maintaining stem cell properties, and thus have confirmed an effect of promoting stem cell activity, thereby completing the present invention.

Means for solving the problems

In order to achieve the above object, the present invention provides a composition for improving skin comprising a white truffle extract as an active ingredient, which is used for cosmetics, foods, medicines or external medicines.

As another means for solving the above problems, the present invention provides a composition for promoting skin stem cell activity, which comprises a white truffle extract as an active ingredient.

In addition, the use of white truffle extract for preparing the above composition is provided.

ADVANTAGEOUS EFFECTS OF INVENTION

The white truffle extract according to the present invention improves skin texture by promoting the activity of skin stem cells, strengthens skin barrier by promoting PPARr expression and/or filaggrin expression, and improves skin moisturizing ability, thereby also having an effect of preventing or treating atopic dermatitis, and thus can be effectively used for cosmetics, foods, pharmaceuticals or pharmaceutical external products for improving skin health and treating skin diseases.

Drawings

FIG. 1 is a photograph comparing actual skin, artificial skin prepared with epidermal stem cells, and artificial skin prepared with ordinary skin cells (J Clin invest.2004;113 (3): 390-400).

Fig. 2 shows the results of confirming the epidermal stem cell growth and division promoting effect (epidermal stem cell activity effect) of the white truffle extract.

FIG. 3 shows the results of confirming the effect of increasing the stem cell activity of the epidermal stem cell of the white truffle extract.

FIG. 4 is a graph showing the analysis of the variation in the expression of silk fibroin mRNA caused by the treatment of white truffle extract.

FIG. 5 is a graph showing the analysis of PPARr mRNA expression changes caused by the treatment of white truffle extract.

Detailed Description

The present invention is described in detail below.

In order to exert excellent effects when applied to actual skin, the ingredients that regenerate skin, improve characteristics, moisturize skin, and improve skin texture preferably exhibit high activity at low concentration, regenerate skin, improve characteristics, moisturize skin, and improve skin texture activity, are excellent in absorption through skin, low in volatility so as to be able to stay for a sufficient time to exhibit effects of regenerating skin, improving characteristics, moisturize skin, and improving skin texture, keep active ingredients stable in or on the composition, be easily prepared into medicines, cosmetics, foods, or the like, and be safe to skin. However, among known components, a component satisfying all of the above characteristics is not common. For example, when some components for regenerating skin, improving characteristics, moisturizing skin, and improving skin texture are tested in vitro, even at low concentrations, the activities of regenerating skin, improving characteristics, moisturizing skin, and improving skin texture are excellent, but the ability to absorb through the skin is reduced, so that it is difficult to apply to actual skin. Other active ingredients are less hydrophilic and therefore difficult to formulate into pharmaceuticals or cosmetics and foods. In addition, some ingredients that regenerate skin, improve characteristics, moisturize skin, and improve skin texture also exist where the above active ingredients are decomposed or deformed into other compounds upon exposure to heat, light, or oxygen, so that the effect has been lost before application to the skin.

From the following examples, it is confirmed that the white truffle extract exhibits remarkably excellent proliferation promoting effect of skin stem cells, ppar activity effect, silk fibroin expression effect at low concentration, and thus can be used as an active ingredient of a pharmaceutical, cosmetic or food composition for improving skin barrier, improving skin texture, improving characteristics and improving skin moisturization.

Accordingly, the present invention provides a cosmetic, food, pharmaceutical external or pharmaceutical composition for improving skin comprising the extract of white truffle.

The white truffle (tube Magnatum) of the invention is one of truffle or American truffle (truffle). There are 30 or more truffles, but among them, black truffle (Tuber Melanosporum) and white truffle (Tuber Magnatum) are most preferable. The growth is carried out at 5-30 cm of the ground of the quercetin, and is found in a depth of 1 m. For collecting the xidatong dew, the smell of dogs or pigs is used. The artificial cultivation is not possible, and the yield is low, so the scarcity is high.

The "skin barrier improving" effect of the present invention means that the skin is thickened by increasing the stem cell property of the epidermal cells, the skin is smoothed by increasing the regenerative power of the skin barrier, and the skin tissue is improved by promoting the differentiation of the epidermal cells, to damage caused by external and internal causes of the skin. The damage caused by the external causes may include ultraviolet rays, external pollutants, wounds, traumas, etc., and the damage caused by the internal causes may include stress (stress) etc., but is not limited thereto.

The "skin texture improving" effect of the present invention means that the skin surface is smoothed by suppressing or blocking the roughness of the skin surface due to the influence of aging, pressure, etc., and the gloss is imparted to improve the skin color.

The "characteristic improving" effect of the present invention shows an effect of preventing and treating skin diseases such as characteristics, xerosis cutis, etc., and means suppression or inhibition or alleviation of symptoms of characteristics.

The "moisturizing" effect of the present invention means that the moisture content of the skin is increased by preventing or suppressing the decrease in the moisture content of the skin, and the skin surface is smoothed and is given luster.

The above white truffle extract can be extracted by methods known in the art, and the method thereof is not particularly limited. Alternatively, a commercially available extract may be used.

The kind of the extraction solvent used for extracting the white truffle is preferably not particularly limited, and any solvent known in the art may be used. As non-limiting examples of the above extraction solvents, water (or distilled water) may be used; lower alcohols of C1 to C4 such as methanol, ethanol, propanol, butanol, etc.; polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; hydrocarbon solvents such as ethyl acetate, acetone, hexane, diethyl ether, and methylene chloride; or a mixture thereof, specifically, water (or distilled water), lower alcohol, 1, 3-butanediol, ethyl acetate may be used alone, or 2 or more kinds may be mixed for use, and more specifically, an aqueous ethanol solution may be used.

The solvent extract may be prepared by extracting white truffle more than 1 time using the above solvents.

In the present invention, the extract prepared as described above or the fraction obtained by subjecting to the above fractionation process may be subjected to filtration or to a concentration or drying process to remove the solvent, and the above filtration, concentration and drying may be performed. Specifically, the filtration may be performed by a filter paper or a reduced pressure filter, the concentration may be performed by reduced pressure concentration using a reduced pressure concentrator or the like, and the drying may be performed by a freeze drying method.

The extract produced as described above or the fraction obtained by the fractionation process described above may be purified by various chromatographic methods such as silica gel column chromatography (silica gel column chromatography), thin layer chromatography (thin layer chromatography) and high performance liquid chromatography (high performance liquid chromatography), to obtain a further purified fraction.

As another aspect, a composition for promoting skin stem cell activity comprising a white truffle (Tuber Magnatum) extract is provided. The composition is a cosmetic, pharmaceutical external, food or pharmaceutical composition for promoting skin stem cell activity.

The above-mentioned composition for promoting skin stem cell activity may preferably be a composition for promoting epidermal stem cell activity.

In the present invention, it was confirmed in experimental example 1 that the epidermal stem cells were activated by 116% when the 0.1ppm white truffle extract was treated for the effect of promoting the activity of the epidermal stem cells.

As another aspect, a composition for promoting expression of pparγ (PPARr) or filaggrin (filaggrin) comprising a white truffle (tub Magnatum) extract is provided.

The "Filaggrin" of the present invention is a protein that plays an important role in the formation of skin barrier, and plays a role in aggregating keratin in keratinocytes, thereby forming a solid skin barrier. It is well known that the fact that barrier function is reduced in aged skin is due to the fact that several filaggrin proteins are reduced. In particular, the fact that atopic dermatitis, a disease that appears to be a dysfunction of the skin barrier, has been reported to be intimately involved in the reduction of silk fibroin (j. Invest. Dermotol., 126:1200-1202,2006.). In addition, the fact that if silk fibroin increases, the moisturizing ability increases is also reported (Biochem Med (Zagreb) 2019;29 (2): 020501., journal of Investigative Dermatology (2020) 140, 615-623.). That is, if the skin barrier is to be enhanced, the expression of the silk fibroin must be increased.

The "PPARr (pparγ)" of the present invention is another protein which has an important correlation with atopic dermatitis, has a very important role in differentiation of skin cells, recovery of barrier function, biosynthesis of lipids, etc., and positively affects the skin by promoting expression of skin barrier proteins, i.e., evolucrin, lorecrin (paphiopediin), etc. In contrast, a decrease in PPARr may lead to damage of the skin barrier, and it was confirmed that the expression of this protein was decreased in atopic dermatitis patients (Our Dermatol Online.5 (2): 107-112, 2014.). Thus, like silk-polyprotein, PPARr, like silk-polyprotein, can repair the skin barrier if expression is increased.

In the present invention, the expression level of PPARr was confirmed in Experimental example 2, and when 0.5ppm of white truffle extract was treated, it was confirmed that PPARr gene was increased 1.32-fold.

In the present invention, the amount of expressed silk fibroin was confirmed in experimental example 2, and when 0.5ppm of white truffle extract was treated, it was confirmed that silk fibroin gene was increased 1.42 times.

The "cosmetic composition" of the present invention may be in the form of a general emulsion or a solubilized form. For example, there may be provided lotions such as a skin softening lotion or a nourishing lotion, lotions such as facial lotions, body lotions, creams such as nourishing creams, moisturizing creams, eye creams, essence, make-up ointments, balm (balm), sprays, gels, masks, sunscreens, pre-makeup base lotions, foundations of liquid, solid or spray type, etc., powders, cleansing agents such as cleansing creams, cleansing lotions, cleansing oils, etc., dosage forms such as cleansing foam, soap, body wash, etc.

In addition, the above-mentioned cosmetics may further contain adjuvants commonly used in the cosmetic field, such as fatty substances, organic solvents, solubilizing agents, concentrates and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents (foaming agents), fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion blocking agents and chelating agents, preserving agents, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics.

The cosmetic dosage form may contain the above white truffle extract at a relatively high concentration for a cosmetic such as cleansing agent, washing agent, etc. in which the active ingredient stays on the skin in a short time. In contrast, in a leave-on cosmetic such as a lotion, an emulsion, a cream, an essence, etc., in which the active ingredient remains on the skin for a long period of time, the above-mentioned white truffle extract may be contained at a concentration lower than that of a rinse-off cosmetic. Although not limited thereto, in one embodiment of the present invention, the above composition may comprise 0.0001 to 10 wt% (preferably 0.0001 to 1 wt%) of the above white truffle extract with respect to the total weight of the composition. When the composition of the present invention contains less than 0.0001% by weight of the above-mentioned white truffle extract, sufficient effects of regenerating skin, improving characteristics, moisturizing skin and improving skin texture cannot be expected, and when it contains more than 10% by weight, undesired reactions such as allergy or the like may occur or there is a problem in skin safety, and thus, it is intended to prevent such problems.

The "food composition" of the present invention is a composition obtained by adding the above-mentioned white truffle extract to food materials such as beverages, teas, spices, chewing gums, biscuits, etc., or preparing into foods such as capsules, powders, suspensions, etc.

Since the above-mentioned food preparation can be taken on a daily basis, it is expected that the food preparation has high effects of regenerating skin, improving characteristics, moisturizing skin and improving skin texture, and is therefore very useful.

When the above white truffle extract is used as a food additive, the above white truffle extract may be added directly or used together with other foods or food ingredients, and may be suitably used according to conventional methods. The mixing amount of the active ingredient may be appropriately determined depending on the purpose of use thereof (prophylactic, healthy or therapeutic treatment). In general, in preparing a food or beverage, the composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, relative to the raw materials. However, in the case of taking for a long time for the purpose of health and hygiene or for the purpose of regulating health, the above amount may be not more than the above range, and since there is no problem in safety, the active ingredient may be used in an amount not less than the above range.

The kind of the above food is not particularly limited. As examples of foods to which the above substances can be added, there are meats, sausages, breads, chocolates, candies, snacks, biscuits, pizzas, instant noodles, other noodles, chewing gums, dairy products containing ice cream, various soups, beverages, teas, oral liquids, alcoholic beverages, vitamin complex agents and the like, and include all general-purpose health foods.

When the food dosage form is a beverage, various flavors or natural carbohydrates, etc., may be contained as additional ingredients like conventional beverages. The natural carbohydrate is monosaccharide such as glucose, fructose, disaccharide such as maltose, sucrose, and polysaccharide such as dextrin, cyclodextrin, xylitol, sorbitol, erythritol, etc. As the sweetener, natural sweeteners such as thaumatin (thaumatin), stevia (stevia) extract, or synthetic sweeteners such as saccharin, aspartame, and the like can be used. The proportion of the above natural carbohydrates is generally from about 0.01 to 0.04g, preferably from about 0.02 to 0.03g, per 100mL of the composition of the invention.

In addition to the above, the food formulation may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acids and salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages, and the like. In addition to this, the food dosage form may contain pulp for preparing natural fruit juices, fruit juice beverages and vegetable beverages. These components may be used alone or in combination. The proportion of these additives is not critical, but is generally selected in the range from 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the invention.

The "pharmaceutical external composition" of the present invention refers to a composition for articles other than an article other than an instrument, a device or an apparatus, among articles used for the purpose of diagnosing, treating, alleviating, disposing or preventing a disease in a human or an animal, and articles other than an article other than an instrument, a device or an apparatus, among articles used for the purpose of pharmacologically affecting the structure and function of a human or an animal, and may include, as a specific example, an internal preparation, but is not limited thereto, and a preparation method, an amount, a utilization method, a constituent component, and the like of a pharmaceutical external preparation may be appropriately selected from conventional techniques known in the art.

The "stem cell" of the present invention is a cell capable of undergoing cell division by itself and capable of differentiating into a specific cell type (specific cell type) of a wide variety of forms. The type of the stem cell is not particularly limited, and in one specific example, the stem cell may be a skin stem cell. The "skin stem cells" mentioned above refer to stem cells capable of differentiating into cells constituting the skin (epidermis, dermis, and subcutaneous fat layer). The cells constituting the above skin include keratinocytes (keratinocyte) present in the epidermis, melanocytes (melanocyte), and fibroblasts present in the dermis (mainly responsible for collagen and elastin biosynthesis).

The kind of the skin stem cells is not particularly limited. The skin stem cells used in the present invention are available regardless of where they originate. For example, the skin stem cells may be obtained from a well-known skin stem cell supply source, such as a basal layer of hair follicle or epidermis, and the animal to be harvested may be a mammal. In one embodiment, the mammal may include, but is not limited to, a human, a mouse, a rat, a guinea pig, a rabbit, a monkey, a pig, a horse, a cow, an sheep, a antelope, a dog, or a cat. Preferably, the mammal may be a human. Such methods of obtaining skin stem cells from a supply of skin stem cells are well known in the art.

In the present invention, the "effect of promoting the activity of stem cells" is meant to include an effect of promoting the proliferation of stem cells, and/or an effect of maintaining the specificity index molecule of stem cells and self-splitting property, i.e., stem cell properties.

In addition, the composition of the present invention can be used to prepare cosmetic dosage forms. These cosmetic formulations are the same as those described in the previous description of cosmetic formulations comprising white truffle extract.

As another aspect, there is provided the use of a white truffle extract for the preparation of cosmetics, foods, pharmaceutical exteriors, and pharmaceutical compositions for skin improvement.

As another aspect, there is provided the use of a white truffle extract for the preparation of a composition for promoting skin stem cell activity.

The composition can be used for promoting proliferation of skin stem cells or improving maintenance capacity of skin stem cells.

As another aspect, there is provided the use of a white truffle extract for the preparation of a composition for promoting expression of pparγ (PPARr) or filaggrin.

Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are merely illustrative of the present invention, and the present invention is not limited to the following examples. In addition, these experimental examples are only for aiding in understanding the present invention, and thus the scope of the present invention is not limited in any sense by them.

Experimental example 1: skin texture improvement by enhancing stem cell properties of epidermal stem cells

First, an attempt was made to confirm the assumption that strengthening skin stem cell properties is more helpful in improving skin texture to set the direction of the experiment, and comparison was made between actual skin and prepared artificial skin (fig. 1).

The structure of actual skin and artificial skin prepared with epidermal stem cells and artificial skin prepared with ordinary skin cells were compared with photographs (source: J Clin invest.2004;113 (3): 390-400). In the photographs a, d, g in fig. 1, if the basal layer (basal layer) marked with red arrows is compared, it can be confirmed that the artificial skin d prepared with the epidermal stem cells shows the dense concentration of cells at the actual skin a level. That is, the artificial skin prepared with the epidermal stem cells is more similar to the actual skin structure than the artificial skin prepared with the ordinary skin of keratinocytes, fibroblasts, etc. Therefore, it is expected that the effect of stimulating keratinocytes and fibroblasts is also expected to some extent, such as skin regeneration and skin texture improvement, but it can be said that it is advantageous to stimulate epidermal stem cells in order to restore the structure most similar to the original structure of actual skin. After that, the epidermal stem cell activity of white truffle was confirmed.

[ A. Epidermal Stem cell Activity ]

Epidermal stem cells (primary human epidermal keratinocytes, primary human epidermal keratinocytes, HPEKp) were purchased from CellnTec (Switzerland) and then cultured in carbon dioxide medium at 37 ℃ under 5% carbon dioxide. 10000 cells were implanted in 96-well plates, and 0.1ppm white truffle was post-treated for 24 hours and further cultured for 24 hours. The degree of cell activity was confirmed using CCK-8 (Dojindo) and is shown graphically in FIG. 2. When 0.1ppm white truffle was treated, 116% activation of epidermal stem cells was confirmed (p < 0.05), and the fact that it was positively helpful to the division of stem cells was seen.

[ comparison of Colony (color) count ]

*65 this experiment was based on the condition that cells having no stem cell property are difficult to divide, and utilized the characteristic that stem cells can grow and divide even if they exist as single cells (single cell survival ). To analyze whether or not the white truffle can increase the epidermal stem cell property, 400 cells were implanted in a 60mm disk cell plate (disk plate) using the same epidermal stem cells as in the A experiment, and 0.1ppm white truffle was treated after 24 hours. For a total of 7 days, the number of colonies stained with crystal violet (sigma) was then counted using image J. It is considered that the more colonies are formed, the greater the stem cell activity is. The results are shown in FIG. 3, in which the colony count was increased 1.2 times in the experimental group treated with white truffle, thereby confirming that white truffle increased epidermal stem cell properties. This finally shows that the white truffle extract shows more excellent efficacy than other materials in terms of skin regeneration and skin texture improvement.

Experimental example 2: effects of repairing skin barrier and improving characteristics

It is attempted to confirm the effect of improving the characteristics by confirming the expression amounts of the silk fibroin and PPARr.

Keratinocytes and fibroblasts were cultured in carbon dioxide medium at 37℃under 5% carbon dioxide. For keratinocytes used to observe the expression of silk fibroin, 30000 cells were seeded in 6-well plates and 0.05ppm white truffle extract was post-treated for 24 hours. After 24 hours again, RNA was obtained using an RNA prep kit (Qiagen, germany). After cDNA was synthesized from RNA using cDNA synthesis kit (cDNA systhesis kit) (philiekorea, korea), the expression level of silk fibroin was analyzed by quantitative PCR. For fibroblasts used to observe PPARr expression, 50000 cells were implanted in a 6-well plate, and 0.5ppm white truffle extract was post-treated for 24 hours. After that, the expression level of PPARr was analyzed by the same experimental method as that of the silk fibroin.

The expression level of silk fibroin is shown in FIG. 4, and the expression level of PPARr is shown in FIG. 5.

When 0.05ppm of the white truffle extract was treated, it was confirmed that the silk fibroin gene was increased 1.42-fold, and that the PPARr gene was increased 1.32-fold.

The increased expression of these proteins means that the skin barrier is repaired quickly and the symptoms of the patient with the characteristics can be improved, so that the extract of white truffle having both effects appears to have very excellent effects on repairing the skin barrier and improving the characteristics.

Claims

1. Use of a composition comprising white truffle (Tuber Magnatum) extract for the preparation of a cosmetic for improving skin.

2. The use according to claim 1, wherein the extract is an extract of one or more solvents selected from the group consisting of water, C1-C4 alcohol, 1, 3-butanediol, and ethyl acetate.

3. The use according to claim 1, wherein the cosmetic is in the form of a lotion, a concentrate, a lotion, a cream, a mask, a gel, a powder, a foundation, a balm, an ointment or a detergent.

4. The use according to claim 1, wherein the skin improvement is at least one selected from the group consisting of skin barrier improvement, skin texture improvement, skin moisturization improvement, and atopic dermatitis improvement.

5. The use according to claim 4, wherein said improvement in skin barrier or skin texture is due to an increase in skin stem cell activity and/or an increase in skin stem cell activity.

6. The use according to claim 4, wherein said improvement in skin barrier, skin moisturization or atopic dermatitis is due to an increase in filaggrin expression and/or PPARr expression.

7. Use of a composition comprising an extract of white truffle (Tuber Magnatum) for the preparation of a food product for improving skin.

8. The use according to claim 7, wherein the extract is an extract of one or more solvents selected from the group consisting of water, C1-C4 alcohol, 1, 3-butanediol, and ethyl acetate.

9. The use according to claim 7, wherein the skin improvement is at least one selected from the group consisting of skin barrier improvement, skin texture improvement, skin moisturization improvement, and atopic dermatitis improvement.

10. The use according to claim 9, wherein said improvement in skin barrier or skin texture is due to an increase in skin stem cell activity and/or an increase in skin stem cell activity.

11. The use according to claim 9, wherein said improvement in skin barrier, skin moisturization or atopic dermatitis is due to an improvement in filaggrin expression and/or PPARr expression.

12. Use of a composition comprising a white truffle (Tuber Magnatum) extract for the preparation of a skin-improving pharmaceutical external product.

13. The use according to claim 12, wherein the extract is an extract of one or more solvents selected from the group consisting of water, C1-C4 alcohol, 1, 3-butanediol, and ethyl acetate.

14. The use according to claim 12, wherein the skin improvement is at least one selected from the group consisting of skin barrier improvement, skin texture improvement, skin moisturization improvement, and atopic dermatitis improvement.

15. The use according to claim 14, wherein said improvement in skin barrier or skin texture is due to an increase in skin stem cell activity and/or an increase in skin stem cell activity.

16. The use according to claim 14, wherein said improvement in skin barrier, skin moisturization or atopic dermatitis is due to an increase in filaggrin expression and/or PPARr expression.

17. Use of white truffle (Tuber Magnatum) extract for preparing composition for promoting skin stem cell activity.

18. Use of a white truffle extract according to claim 17 wherein said composition is used for promoting proliferation of skin stem cells or improving maintenance of skin stem cells.

19. Use of a white truffle (Tuber Magnatum) extract for the preparation of a composition for promoting expression of pparγ (PPARr) or filaggrin (filaggrin).