CN115990328B - Composite preparation for efficiently degrading soil glyphosate, and use method and application thereof - Google Patents

Abstract

The invention discloses a composite preparation for efficiently degrading soil glyphosate, and a use method and application thereof. The active ingredients of the composite preparation are carbamide peroxide and ferrous sulfate, and the concentration ratio of the carbamide peroxide to the ferrous sulfate in the composite preparation is 1-3: 0.5 to 1. The compound preparation can efficiently degrade soil glyphosate, ensures the supply of nitrogen nutrients to the soil, ensures that the degradation rates of the glyphosate are 31.71%, 46.66%, 50.24% and 70.74% respectively after the 1 st, 7 th, 14 th and 28 th days after application, and simultaneously increases the ammonium nitrogen content of the soil, so that fertilization and degradation of the glyphosate are completed in one step, and the compound preparation has the characteristics of no increase of production cost and labor cost, easiness in operation, no secondary pollution and the like, and has good theoretical and application popularization values.

Description

A composite preparation for efficiently degrading soil glyphosate and its use method and application

Technical Field

The present invention belongs to the field of soil remediation and agricultural technology, and specifically relates to a composite preparation for efficiently degrading soil glyphosate, and a use method and application thereof.

Background technique

Glyphosate [N-(phosphomethyl)glycine] is a broad-spectrum and highly effective herbicide that provides protection for eliminating weeds in farmland and increasing the income and yield of grain crops. However, with the widespread use and irrational application of glyphosate, the problem of glyphosate residues in farmland soil and water bodies has become increasingly prominent. In recent decades, many studies have shown that glyphosate or herbicides based on glyphosate can have toxic effects on non-target aquatic organisms, including microorganisms, such as amphibians, invertebrates, aquatic plants, shrimp, etc. Glyphosate can even have toxic effects on humans after being transmitted through the food chain. On October 27, 2017, the World Health Organization's International Agency for Research on Cancer published a preliminary list of carcinogens for reference, and glyphosate is on the list of Class 2A carcinogens.

At present, the method for glyphosate degradation is mainly through microbial degradation. However, most of the degradation bacteria screened out in most patents are single strains, or two to three strains. Microbial degradation of glyphosate is still limited to the laboratory research stage, and in complex soil systems, there are a large number of soil indigenous (pioneer) microbial strains, and it is difficult to ensure the effect of artificially added degradation bacteria on glyphosate degradation. There is little research on how to achieve glyphosate degradation while increasing farmland fertility. Therefore, it is very important to provide a composite preparation and method that is efficient, economical, simple and easy to operate, and can supplement fertilizer and efficiently degrade soil glyphosate.

Summary of the invention

The purpose of the present invention is to provide a composite formulation for efficiently degrading soil glyphosate and a method for using and application thereof in view of the above-mentioned deficiencies in the prior art.

The first object of the present invention is to provide a composite formulation for efficiently degrading soil glyphosate.

Another object of the present invention is to provide the application of the above-mentioned highly efficient soil degradation glyphosate composite formulation.

Another object of the present invention is to provide a method for using the above-mentioned highly efficient soil-degrading glyphosate composite formulation.

The above-mentioned purpose of the present invention is achieved through the following technical solutions:

A composite preparation for efficiently degrading soil glyphosate, wherein the active ingredients of the composite preparation are urea peroxide and ferrous sulfate.

The present invention aims to utilize urea peroxide with oxygen supply and slow release and ferrous sulfate to prepare a composite preparation that can autonomously trigger a (quasi) Fenton effect to effectively degrade soil glyphosate. The composite preparation generates soil micromolar concentration of hydrogen peroxide through the slow release of urea peroxide, and efficiently promotes the degradation of glyphosate by optimizing the catalytic effect of Fe ²⁺ . The composite preparation can not only efficiently degrade soil glyphosate, but also ensure the supply of soil nutrients, and is a method that is simple to operate and reduces labor input.

Preferably, the concentration ratio of the urea peroxide to the ferrous sulfate is 1-3:0.5-1.

More preferably, the concentration ratio of the urea peroxide to the ferrous sulfate is 1 to 2:1.

Preferably, the content of urea peroxide in the composite preparation is 2-6 mmol/kg, and the content of ferrous sulfate is 1-2 mmol/kg.

Preferably, the nitrogen content of the urea peroxide is 28-32%, the active oxygen mass fraction is 12-16%, and the hydrogen peroxide content is 34-38%.

More preferably, the nitrogen content of the urea peroxide is 30-31%, the active oxygen mass fraction is 14-15%, and the hydrogen peroxide content is 35-36%.

Preferably, the ferrous sulfate salt includes ferrous sulfate heptahydrate or ferrous sulfate.

Preferably, the composite formulation can be formulated with 10-35 mg/kg of a soil conditioner; the soil conditioner comprises the following raw materials in parts by weight: 10-19 parts of nitrogen fertilizer, 6-19 parts of phosphorus fertilizer, and 5-34 parts of potassium fertilizer.

Preferably, the nitrogen fertilizer is one or more of urea, ammonium sulfate, and ammonium phosphate; the phosphate fertilizer is one or more of superphosphate and calcium magnesium phosphate; and the potassium fertilizer is one or more of potassium sulfate and potassium chlorate.

The present invention also provides the use of the composite formulation in the preparation of a soil degrading glyphosate product. In a preferred embodiment of the present invention, in the early stage of cultivation, the UHP and Fe+UHP treatment groups can remove glyphosate in paddy soil and red soil more quickly and with better effects.

Preferably, the pH of the soil is 6.0-7.0.

Preferably, the soil is southern red soil or paddy soil. The present invention is particularly suitable for paddy soil and southern red soil contaminated by glyphosate, and can simultaneously achieve glyphosate degradation and nitrogen fertilizer application.

The present invention also provides a method for using the composite preparation, which mainly comprises the following steps:

S1. Select the soil affected by glyphosate, dry it and turn it evenly; the glyphosate content is 8-15 mg/kg;

S2. Add the composite preparation to the contaminated soil and mix well; the application rate is 12 to 15 kg per mu;

S3. After applying the compound preparation, water/irrigate to maintain the soil moisture content at 30% to 60%;

S4. Transplant crops 5-10 days after fertilization.

Preferably, the glyphosate content is 10-11 mg/kg.

Preferably, crops are transplanted 7-8 days after fertilization.

The present invention selects urea peroxide (UHP) and ferrous sulfate (Fe) to mediate Fenton reaction or Fenton-like reaction to promote the degradation of soil glyphosate, which has a better effect. On the 1st, 7th, 14th and 28th days of cultivation, the degradation rates of glyphosate in the Fe+UHP group were 31.71%, 46.66%, 50.24% and 70.74%, respectively, which were significantly higher than those in other treatment groups. After the first day of application, the ammonium nitrogen content of the UHP and Fe+UHP in the nitrogen application group of paddy soil increased by 2.86 times and 2.84 times respectively compared with the control group; the ammonium nitrogen content of the UHP and Fe+UHP in the red soil increased by 3.73 times and 3.15 times respectively compared with the control group. The ammonium nitrogen variation ranges of paddy soil and red soil were 11.29-100.03 mg/kg and 2.73-73.19 mg/kg, respectively. Compared with the control, the ammonium nitrogen content in the nitrogen application group was significantly higher during the 28-day incubation period, and the ammonium nitrogen content and urease activity in the soil were greatly increased, and the activities of nitrate reductase and catalase were reduced.

In the present invention, the ammonium nitrogen content in paddy soil is generally higher than that in red soil; moreover, when urea peroxide, urea and ferrous sulfate are applied to glyphosate-contaminated soil, the nitrogen conversion of the nitrogen-applying group is relatively small, and nitrogen nutrition can be normally provided while removing glyphosate; secondly, compared with the control group, the application of the compound preparation is beneficial to the underground growth of corn seedlings and promotes the growth of its stem thickness, wherein urea peroxide can significantly increase the root length of the seedlings. In addition, compared with the control group, there is no significant difference in nitrogen conversion between the nitrogen-applying groups of urea peroxide and ordinary urea, but urea peroxide can promote faster degradation of glyphosate residues, reduce the toxicity of glyphosate residues while providing nitrogen nutrition normally, and promote corn growth.

Compared with the prior art, the present invention has the following beneficial effects:

Compared with the commonly used method of microbial degradation of soil glyphosate, the present invention provides a composite preparation for efficiently degrading soil glyphosate and its use method and application. By using urea peroxide and ferrous sulfate with oxygen supply and slow release to form a composite preparation that can autonomously trigger (quasi) Fenton effect, the soil glyphosate can be effectively degraded. The method can not only efficiently degrade soil glyphosate, but also ensure the supply of soil nutrients, and is a method that is simple to operate and reduces labor input. The present invention solves the problem that it is difficult to balance the restoration of glyphosate-contaminated farmland soil and agricultural fertilization, has good environmental benefits, economic benefits and social benefits, and has good promotion and application value.

BRIEF DESCRIPTION OF THE DRAWINGS

Figure 1 shows the residual concentration of glyphosate in paddy soil after the application of urea peroxide and ferrous sulfate.

Figure 2 shows the ammonium nitrogen content in paddy soil after the application of urea peroxide and ferrous sulfate.

Figure 3 shows the residual concentration of glyphosate in southern red soil after applying urea peroxide and ferrous sulfate.

Figure 4 shows the ammonium nitrogen content in red soil after the application of urea peroxide and ferrous sulfate.

Figure 5 is an experimental picture of paddy soil and red soil after applying urea peroxide and ferrous sulfate.

Figure 6 is a photo of the growth of corn after the application of urea peroxide and ferrous sulfate.

Figure 7 is a graph of agronomic traits of corn seedlings in glyphosate-contaminated soil under different fertilizer ratios; among them, (a) plant height; (b) stem thickness; (c) root length; (d) aboveground fresh weight; (e) aboveground dry weight; (f) underground fresh weight; and (g) underground dry weight.

Detailed ways

The present invention is further described below in conjunction with specific examples, but the examples do not limit the present invention in any form. Without departing from the spirit and essence of the present invention, simple modifications or replacements made to the methods, steps or conditions of the present invention are within the scope of the present invention; unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing implementation modes and embodiments and are not intended to limit the present invention.

the term

Unless otherwise specified or incompatible herewith, the terms and phrases used herein shall have the following meanings:

The terms "and/or", "or/and", and "and/or" used in this article have a selection range that includes any one of two or more related listed items, and also includes any and all combinations of related listed items, and the arbitrary and all combinations include any combination of two related listed items, any more related listed items, or all related listed items. It should be noted that when at least three items are connected by at least two conjunctions selected from "and/or", "or/and", and "and/or", it should be understood that in this application, the technical solution undoubtedly includes technical solutions that are all connected by "logical and", and undoubtedly includes technical solutions that are all connected by "logical or". For example, "A and/or B" includes three parallel solutions of A, B and the combination of A and B. For example, the technical solution of "A, and/or, B, and/or, C, and/or, D" includes any one of A, B, C, and D (that is, the technical solution that is all connected by "logical OR"), and also includes any and all combinations of A, B, C, and D, that is, the combination of any two or any three of A, B, C, and D, and also includes the combination of four of A, B, C, and D (that is, the technical solution that is all connected by "logical AND").

Herein, "preferred", "better", "more preferred" and "suitable" are only used to describe implementation methods or examples with better effects, and it should be understood that they do not constitute a limitation on the scope of protection of the present invention. If multiple "preferred" items appear in a technical solution, unless otherwise specified and there is no contradiction or mutual restriction, each "preferred" item is independent.

In the present invention, “further”, “furthermore”, “particularly”, etc. are used for descriptive purposes to indicate differences in content, but should not be construed as limiting the scope of protection of the present invention.

In the present invention, "optionally", "optional", and "optional" mean optional, that is, any one of the two parallel solutions of "yes" or "no". If multiple "options" appear in a technical solution, unless otherwise specified and there is no contradiction or mutual restriction, each "optional" is independent.

In the present invention, in the "first aspect", "second aspect", "third aspect", "fourth aspect", etc., the terms "first", "second", "third", "fourth", etc. are used only for descriptive purposes and cannot be understood as indicating or implying relative importance or quantity, nor can they be understood as implicitly indicating the importance or quantity of the indicated technical features. Moreover, "first", "second", "third", "fourth", etc. only serve the purpose of non-exhaustive enumeration and description, and it should be understood that they do not constitute a closed limitation on quantity.

In the present invention, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.

In the present invention, when it comes to a numerical interval (i.e., a numerical range), unless otherwise specified, the distribution of the optional numerical values in the numerical interval is considered to be continuous, and includes the two numerical endpoints (i.e., the minimum value and the maximum value) of the numerical interval, and each numerical value between the two numerical endpoints. Unless otherwise specified, when the numerical interval only refers to the integers in the numerical interval, including the two endpoint integers of the numerical range, and each integer between the two endpoints, is equivalent to directly listing each integer. When multiple numerical ranges are provided to describe features or characteristics, these numerical ranges can be merged. In other words, unless otherwise specified, the numerical range disclosed herein should be understood to include any and all sub-ranges included therein. The "numerical value" in the numerical interval can be any quantitative value, such as a number, a percentage, a ratio, etc. "Numerical interval" allows broadly including numerical interval types such as percentage intervals, ratio intervals, and ratio intervals.

The temperature parameters in the present invention, if not specifically limited, are allowed to be either constant temperature treatment or to vary within a certain temperature range. It should be understood that the constant temperature treatment allows the temperature to fluctuate within the precision range controlled by the instrument. Fluctuations within the range of ±5°C, ±4°C, ±3°C, ±2°C, and ±1°C are allowed.

In the present invention, the term "room temperature" or "normal temperature" generally refers to 4°C to 35°C, for example, 20°C ± 5°C. In some embodiments of the present invention, "room temperature" or "normal temperature" refers to 10°C to 30°C. In some embodiments of the present invention, "room temperature" or "normal temperature" refers to 20°C to 30°C.

In the present invention, when referring to the unit of the data range, if there is a unit only after the right endpoint, it means that the units of the left endpoint and the right endpoint are the same. For example, 3-5h means that the units of the left endpoint "3" and the right endpoint "5" are both h (hours).

All documents mentioned in the present invention are cited as references in this application, just as each document is cited as a reference separately. Unless they conflict with the invention purpose and/or technical solution of the present application, the cited documents involved in the present invention are cited with all contents and all purposes. When the present invention involves cited documents, the definitions of relevant technical features, terms, nouns, phrases, etc. in the cited documents are also cited. When the present invention involves cited documents, the examples and preferred embodiments of the cited relevant technical features may also be incorporated into this application as references, but are limited to the ability to implement the present invention. It should be understood that when the content of the citation conflicts with the description in this application, the present application shall prevail or be modified adaptively according to the description of this application.

The mass or weight of the relevant components mentioned in the description of the embodiments of the present invention may not only refer to the specific content of each component, but also indicate the proportional relationship of the mass or weight between the components. Therefore, as long as the content of the relevant components is proportionally enlarged or reduced according to the description of the embodiments of the present invention, it is within the scope disclosed in the description of the embodiments of the present invention. Specifically, the mass or weight described in the description of the embodiments of the present invention may be units known in the chemical industry such as μg, mg, g, kg, etc.

The urea peroxide in the present invention is of agricultural grade or industrial grade, wherein the nitrogen content is 30%, the active oxygen mass fraction is 14%, and the hydrogen peroxide content is 36%.

Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

Example 1 A composite formulation for efficiently degrading glyphosate in rice soil and its use method

The paddy soil in the Zengcheng teaching and research base of South China Agricultural University, where rice, corn and other crops are planted all year round, was used as the experimental soil. After testing, the organic matter content in the basic physical and chemical properties of the paddy soil was 18.15 g/kg, the effective nitrogen, phosphorus and potassium contents were 18.18, 6.96 and 32.10 mg/kg respectively, the gravel, clay and silt were 56.75%, 9.00% and 0.10% respectively, the pH value was 6.10, the Eh value (oxidation-reduction potential) was 237 mV, and the Ec value (conductivity) was 223 μs/cm.

The specific steps include:

S1. Take the topsoil (0-15 cm), air-dry it, grind it into powder, and then pass it through a 2 mm sieve for later use;

S2. The sieved soil was placed into small white buckets with a height of 11.7 cm, a top diameter of 12 cm, and a bottom diameter of 10.4 cm. Each bucket contained 1 kg of soil containing 10 mg/kg of glyphosate.

S3. Add fertilizer to the soil and mix according to the fertilizer ratio in Table 1 below;

S4. Add water to maintain the moisture content of paddy soil at 30%, and maintain the moisture content by weighing every 2 days;

S5. After 1 day, 7 days, 14 days and 28 days of cultivation, take the mixed soil sample in the small bucket and extract it, and then measure it by high performance liquid chromatography.

Table 1 The ratio of urea and ferrous sulfate in different compound preparations

Note: CK is the blank group, without adding ferrous sulfate, urea peroxide and ordinary urea; Fe is only adding 3mmol/kg of ferrous sulfate heptahydrate, UHP is only adding 3mmol/kg of urea peroxide; U is only adding ordinary urea with the same nitrogen content; Fe+UHP is adding 3mmol/kg of ferrous sulfate heptahydrate and urea peroxide; Fe+U is adding 3mmol/kg of ferrous sulfate heptahydrate and urea. The same below.

Table 2 Organic matter content of paddy soil after application

Note: Data with the same lowercase letters between different treatments were not significantly different at p < 0.05 (mean ± s.e.m., n = 3).

As shown in Figure 1, Table 2 and Figure 2, in the paddy soil contaminated with glyphosate, the glyphosate degradation rates of the treatment group (Fe+UHP) with the addition of ferrous sulfate and urea peroxide were 31.71%, 46.66%, 50.54% and 70.74% respectively after the 1st, 7th, 14th and 28th days of cultivation, and the glyphosate removal efficiency was significantly higher than that of the other treatment groups. The glyphosate residues in the CK group were significantly higher than those in the other treatment groups on the 1st, 14th and 28th days. Among them, the glyphosate residue concentration in the system with the combination of ferrous sulfate and urea peroxide (Fe+UHP) decreased the fastest.

Under different treatment conditions, the organic matter content of paddy soil did not reach a significant difference level during the incubation period (p>0.05). The organic matter content in paddy soil was in the range of 15.9-20.99 mg/kg. However, all treatment groups slowly increased with the extension of incubation time, and then tended to a stable dynamic change trend.

During the entire reaction period, the ammonium nitrogen content of the treatment groups with nitrogen addition (UHP group, U group, Fe+UHP group, Fe+U group) was significantly higher than that of the treatment groups without urea addition, and the ammonium nitrogen content of the nitrogen-added groups showed a trend of first increasing and then slowly decreasing. From the first day to the seventh day, the ammonium nitrogen content of all treatment groups increased to varying degrees. CK, Fe, UHP, U, Fe+UHP and Fe+U increased by 39.10%, 63.45%, 24.54%, 21.12%, 15.36% and 18.96% on the seventh day compared with the first day, respectively. Within 28 days, the ammonium nitrogen content of paddy soil varied from 11.29 to 100.03 mg/kg.

These results show that the application of urea peroxide and ferrous sulfate can mediate the Fenton reaction or Fenton-like reaction to promote the degradation of soil glyphosate. In the early stage of cultivation, the Fe+UHP treatment group can quickly remove glyphosate from paddy soil, which can be used as one of the agronomic measures for in situ remediation. At the same time, applying urea peroxide and ferrous sulfate to glyphosate-contaminated soil can provide nitrogen nutrition normally while removing glyphosate.

Example 2 A composite formulation for efficiently degrading glyphosate in southern red soil and its use method

The red soil of the experimental base of the College of Horticulture of South China Agricultural University was used as the experimental soil. The red soil was collected from wasteland with many weeds. According to the test, the organic matter content of the basic physical and chemical properties of the red soil was 13.38g/kg, the effective nitrogen, phosphorus and potassium contents were 11.29, 17.20 and 5.27mg/kg respectively, the gravel, clay and silt were 55.74%, 46.16% and 0.10% respectively, the pH value was 6.90, the Eh value (oxidation-reduction potential) was 196mV, and the Ec value (conductivity) was 384μs/cm.

The specific steps include:

S1. Take the topsoil (0-15 cm), air-dry it, grind it into powder, and then pass it through a 2 mm sieve for later use;

S2. The sieved soil was placed in small white buckets with a height of 11.7 cm, a top diameter of 12 cm, and a bottom diameter of 10.4 cm. Each bucket contained 1 kg of soil containing 10 mg/kg glyphosate.

S3. Add fertilizer to the soil and mix well according to the fertilizer ratio in Table 1 above.

S4. Add water to maintain the water content of the red soil at 30%, and maintain the water content by weighing every 2 days;

S5. After 1 day, 7 days, 14 days and 28 days of cultivation, take the mixed soil sample in the small bucket and extract it, and then measure it by high performance liquid chromatography.

Table 3 Organic matter content of red soil after application

As shown in Figure 3, Table 3, Figure 4 and Figure 5, in the red soil contaminated with glyphosate, in the initial stage of the reaction (day 1), the glyphosate residues in this embodiment are from high to low: Fe>U>CK>Fe+UHP>Fe+U>UHP, and the glyphosate degradation rates are 7.88%, 14.58%, 25.97%, 34.73%, 36.14% and 51.66%, respectively. On the 1st and 7th days after cultivation, the organic matter content of the 6 treatment groups of red soil did not change much, and then the soil increased sharply on the 14th day and then slowly decreased. The ammonium nitrogen content of the treatment group after nitrogen addition was significantly higher than that of the treatment group without nitrogen addition, and the ammonium nitrogen content of the nitrogen application group showed an overall downward trend with the extension of the reaction time. There was no significant difference in the ammonium nitrogen content between the treatment groups without nitrogen addition (CK group and Fe group) at each sampling time (p>0.05). On the first day, the ammonium nitrogen content of the Fe+UHP group was significantly higher than that of the other treatment groups on the 7th and 14th days. The range of ammonium nitrogen content in red soil was 2.37-73.19 mg/kg. A comprehensive comparison of the glyphosate concentrations of the two soils showed that after the application of fertilizers, red soil was easier to remove glyphosate from the soil than paddy soil.

Example 3 Effect of a composite formulation for efficiently degrading glyphosate in soil on corn growth

The red soil was collected from the experimental base of the College of Horticulture of South China Agricultural University, and the paddy soil was collected from the Zengcheng teaching and research base of South China Agricultural University. The red soil was collected from wasteland with many weeds; paddy soil is used to grow rice, corn and other crops all year round. According to tests, the organic matter content in the basic physical and chemical properties of paddy soil is 18.15g/kg, the effective nitrogen, phosphorus and potassium contents are 18.18, 6.96 and 32.10mg/kg respectively, the gravel, clay and silt are 56.75%, 9.00% and 0.10% respectively, the pH value is 6.10, the Eh value (oxidation-reduction potential) is 237mV, and the Ec value (conductivity) is 223μs/cm. The basic physical and chemical properties of red soil include organic matter content of 13.38 g/kg, effective nitrogen, phosphorus and potassium contents of 11.29, 17.20 and 5.27 mg/kg respectively, gravel, clay and silt contents of 55.74%, 46.16% and 0.10% respectively, pH value of 6.90, Eh value (oxidation-reduction potential) of 196 mV and Ec value (conductivity) of 384 μs/cm.

The specific steps include:

S1. Take the topsoil (0-15 cm), air-dry it, grind it into powder, and then pass it through a 2 mm sieve for later use;

S2. The sieved soil was placed into small white buckets with a height of 11.7 cm, a top diameter of 12 cm, and a bottom diameter of 10.4 cm. Each bucket contained 1 kg of soil containing 10 mg/kg glyphosate.

S3. Add fertilizer to the soil and mix well according to the fertilizer ratio in Table 1 above, with 3 replicates for each treatment;

S4. Transplanting corn seedlings 7 days after fertilization treatment;

S5. Take samples from corn 7 days after transplantation and measure the agronomic traits of corn.

As shown in Figures 6 and 7, applying urea peroxide or urea mixed with ferrous sulfate can increase the plant height of corn seedlings in paddy soil and red soil. Applying urea peroxide alone can increase the root length of corn seedlings in paddy soil, and applying urea alone can reduce the root length of corn in paddy soil and red soil.

Embodiments 4 to 8

Examples 4 to 8 and Example 1 were treated under the same environmental conditions, the same management measures, and the same spraying method. The treatment reagent formulas of Examples 4 to 8 are as follows:

Table 4 Compositions of different embodiments of Examples 4 to 8 and glyphosate degradation rates after 28 days

In summary, the present invention uses urea peroxide and ferrous sulfate to mediate the Fenton reaction or Fenton-like reaction to promote the degradation of soil glyphosate. In the early stage of cultivation, the Fe+UHP treatment group can quickly remove glyphosate in paddy soil and red soil, and the effect is good, which can be used as one of the agronomic measures for in-situ remediation and management. Applying urea peroxide and ferrous sulfate to glyphosate-contaminated soil will affect the physical and chemical properties of the soil, but the nitrogen conversion of the nitrogen-applying group is less different, and nitrogen nutrition can be provided normally while removing glyphosate. Applying urea peroxide before planting corn seedlings can reduce the glyphosate concentration in the two soils, and the application of urea peroxide is beneficial to the growth of the underground part of corn seedlings.

The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims (6)

1. The application method of the composite preparation for efficiently degrading the soil glyphosate is characterized by comprising the following steps of:

s1, selecting soil affected by glyphosate, airing and uniformly stirring; the content of glyphosate is 8-15 mg/kg;

S2, adding the composite preparation into polluted soil, and uniformly stirring; the application amount is 12-15 kg per mu;

s3, after the composite preparation is applied, watering/irrigating to keep the water content of the soil to be 30% -60%;

s4, transplanting crops after fertilizing for 5-10 days;

The compound preparation consists of urea peroxide and ferrous sulfate, wherein the concentration ratio of the urea peroxide to the ferrous sulfate is 1-2: 1, a step of; the content of urea peroxide in the composite preparation is 2-6 mmol/kg, and the content of ferrous sulfate salt is 1-2 mmol/kg.

2. The use method according to claim 1, wherein the urea peroxide has a nitrogen content of 28-32%, an active oxygen mass fraction of 12-16% and a hydrogen peroxide content of 34-38%; the ferrous sulfate comprises ferrous sulfate heptahydrate or ferrous sulfate.

3. The method of use according to claim 1, wherein the composite formulation is formulated with 10-35 mg/kg of soil conditioner; the soil conditioner comprises the following raw materials in parts by weight: 10-19 parts of nitrogen fertilizer, 6-19 parts of phosphate fertilizer and 5-34 parts of potash fertilizer.

4. A method of use according to claim 3, wherein the nitrogen fertilizer is one or more of urea, ammonium sulphate, ammonium phosphate; the phosphate fertilizer is one or more of calcium superphosphate and calcium magnesium phosphate fertilizer; the potash fertilizer is one or more of potassium sulfate and potassium chlorate.

5. The method of claim 1, wherein the soil has a pH of 6.0 to 7.0.

6. The method of claim 1, wherein the soil is southern red soil or paddy soil.