CN115943962B - Sterilization composition for preventing and treating banded sclerotial blight of wheat - Google Patents
Sterilization composition for preventing and treating banded sclerotial blight of wheat Download PDFInfo
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- CN115943962B CN115943962B CN202310011769.2A CN202310011769A CN115943962B CN 115943962 B CN115943962 B CN 115943962B CN 202310011769 A CN202310011769 A CN 202310011769A CN 115943962 B CN115943962 B CN 115943962B
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- 125000004170 methylsulfonyl group Chemical group [H]C([H])([H])S(*)(=O)=O 0.000 description 2
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- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 1
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- FBOUIAKEJMZPQG-BLXFFLACSA-N diniconazole-M Chemical compound C1=NC=NN1/C([C@H](O)C(C)(C)C)=C/C1=CC=C(Cl)C=C1Cl FBOUIAKEJMZPQG-BLXFFLACSA-N 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- VMNULHCTRPXWFJ-UJSVPXBISA-N enoxastrobin Chemical compound CO\C=C(\C(=O)OC)C1=CC=CC=C1CO\N=C(/C)\C=C\C1=CC=C(Cl)C=C1 VMNULHCTRPXWFJ-UJSVPXBISA-N 0.000 description 1
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- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
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- STJLVHWMYQXCPB-UHFFFAOYSA-N propiconazole Chemical compound O1C(CCC)COC1(C=1C(=CC(Cl)=CC=1)Cl)CN1N=CN=C1 STJLVHWMYQXCPB-UHFFFAOYSA-N 0.000 description 1
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- WOSNCVAPUOFXEH-UHFFFAOYSA-N thifluzamide Chemical compound S1C(C)=NC(C(F)(F)F)=C1C(=O)NC1=C(Br)C=C(OC(F)(F)F)C=C1Br WOSNCVAPUOFXEH-UHFFFAOYSA-N 0.000 description 1
- KUAZQDVKQLNFPE-UHFFFAOYSA-N thiram Chemical compound CN(C)C(=S)SSC(=S)N(C)C KUAZQDVKQLNFPE-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticides, and in particular relates to a bactericidal composition for preventing and treating banded sclerotial blight of wheat. A bactericidal composition for preventing and treating wheat sheath blight is prepared by compounding flutriafol and penflufen or fluopicolide. The sterilization composition has different action mechanisms of the flutriafol and the penflufen or the fluopicolide, and can inhibit pathogenic bacteria from different action sites when being used in combination, so that the development of pathogen resistance can be delayed, and the service life of the medicament can be prolonged. In addition, the bactericidal composition disclosed by the invention has a synergistic effect on inhibiting rhizoctonia cerealis which is a pathogenic bacterium of the sheath blight of wheat, and can improve the control effect on the sheath blight of wheat, so that the application dosage of pesticides can be reduced, and the control cost is reduced.
Description
Technical Field
The invention belongs to the technical field of pesticides, and in particular relates to a bactericidal composition for preventing and treating banded sclerotial blight of wheat.
Background
Wheat is an important grain crop in China. Currently, banded sclerotial blight of wheat caused by wheat infestations with rhizoctonia cerealis (Rhizoctonia cerealis) has become a worldwide soil-borne bacterial disease. According to incomplete statistics, wheat can generally cause 5-40% yield loss after being damaged by banded sclerotial blight, and even cause no harvest of particles when serious. Meanwhile, due to the lack of disease-resistant wheat varieties stable to the sheath blight of wheat and the reasons of global warming, increased field nitrogen fertilizer consumption, straw returning and the like in recent years, the disease becomes an important disease in main wheat producing areas of Henan province, shandong province, anhui province and the like in China, and the quality and the high yield of the wheat are seriously affected. The prior reports on the prevention and treatment of the banded sclerotial blight of wheat include: thifluzamide, tebuconazole, diniconazole, hexaconazole, propiconazole and the like, but the resistance problem develops rapidly due to continuous single use for many years, and the detection rate of the resistance strain rises sharply, so that the control effect is reduced year by year. Therefore, it is necessary to develop new alternative agents or new formulations.
The penflufen is a pyrazole amide fungicide developed by Bayer, is a succinic dehydrogenase inhibitor, mainly acts on a respiratory chain electron transfer complex II, blocks energy metabolism, and can be used for preventing and treating soil-borne or seed-borne diseases caused by basidiomycota fungi and ascomycota fungi of various crops such as potatoes, corns, paddy rice, cotton, wheat, alfalfa, vegetables, rapes and the like, for example: potato black nevus, wheat sheath blight, rice sheath blight, corn small spot and other diseases.
Fluopicolide is a pyridinamide-based bactericide developed by bayer that acts on ghost proteins, affecting cell mitosis. It is mainly used for crops such as rice, has excellent activity on most bacteria of basidiomycetes, and has good control effect on plant diseases caused by rhizoctonia, such as rice sheath blight disease, rice sclerotinia rot, southern blight and the like.
The flutriafol is triazole sterol demethylation inhibitor bactericide developed by Zhengda group Co., ltd. The flutriafol can effectively inhibit the biosynthesis of ergosterol, cause rupture of fungal cell walls, has broad-spectrum antibacterial activity and stronger systemic property, and has good protection and treatment effects on a plurality of fungal diseases caused by basidiomycetes and ascomycetes. The prior art discloses the compounding of flutriafol and other chemical agents, for example, CN201410788209.9, discloses a compound composition containing methanesulfonyl bacteria and flutriafol and a bactericide, and particularly discloses that after the compounding of the methanesulfonyl bacteria and the flutriafol, the composition can be used for preventing and treating bacterial and fungal diseases, such as rice bacterial leaf streak, citrus canker, tomato bacterial wilt, peanut leaf spot and the like. In addition, CN201310061385.8 also discloses a bactericidal composition containing fluoxastrobin, and specifically discloses a bactericidal spectrum which has higher activity on various diseases of various crops and obvious synergy after the fluoxastrobin and flutriafol are compounded.
At present, no report is reported on the compounding of flutriafol and carbendazim, thiram or enestroburin.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.
Disclosure of Invention
The invention aims to provide a bactericidal composition for preventing and treating wheat sheath blight, which can improve the preventing and treating effect on the wheat sheath blight so as to solve the problems existing when a single chemical agent is used.
In order to achieve the above purpose, the present invention provides the following technical solutions:
The invention provides a sterilization composition for preventing and treating wheat sheath blight, which is prepared by compounding tebuconazole and penflufen or fluopicolide.
Preferably, the mass ratio of the flutriafol to the penflufen is 1-40:30-1.
Preferably, the mass ratio of the flutriafol to the fluopicolide is 1-15:15-1.
The second object of the invention is to provide a bactericide which comprises the bactericidal composition for preventing and treating the sheath blight of wheat and auxiliary components. Wherein the auxiliary component is one or more selected from dispersing agent, wetting agent, emulsifying agent, solvent, binder, disintegrating agent, thickening agent, filler, antifreezing agent, stabilizer, antiseptic and defoaming agent.
Compared with the prior art, the invention has the following beneficial effects:
(1) The sterilization composition has different action mechanisms of the flutriafol and the penflufen or the fluopicolide, and can inhibit pathogenic bacteria from different action sites when being used in combination, so that the development of pathogen resistance can be delayed, and the service life of the medicament can be prolonged.
(2) The bactericidal composition provided by the invention has a synergistic effect on inhibiting rhizoctonia cerealis which is a pathogenic bacterium of the sheath blight of wheat, and can improve the control effect on the sheath blight of wheat, so that the application dosage of pesticides can be reduced, and the control cost is reduced.
Detailed Description
The invention will be better understood from the following examples. However, it will be readily appreciated by those skilled in the art that the description of the embodiments is provided for illustration only and should not limit the invention as described in detail in the claims.
Examples: screening of control agents
1. Test strain: rhizoctonia cerealis (Rhizoctonia cerealis) is obtained from a wheat strain infected in a heart-beating continuous experimental base of Langmuir temple of New county, henan province, and is separated in a laboratory and stored on a PDA culture medium.
2. Test agent
95% Flutriafol (commercially available), 95% penflufen (commercially available) and 97% fluopicolide (commercially available)
Dissolving the test agent with dimethyl sulfoxide, diluting with 0.1% Tween-80 aqueous solution to obtain single-agent mother solution, and setting multiple groups of ratios, wherein each single-agent and the ratio mixture have 5 mass concentration gradients according to an equal ratio method.
3. Test method
The hypha growth rate method is adopted. Adding 9mL of pre-melted PDA culture medium into a sterile conical flask, sequentially quantitatively sucking 1mL of liquid medicine from low concentration to high concentration, respectively adding into the conical flask, shaking uniformly, pouring into a culture dish with the diameter of 9cm, and preparing a medicine-containing flat plate with corresponding concentration; treatments without agent were also included as blank, and 10 replicates were set for each treatment. Cutting bacterial cake at the edge of bacterial colony of tested bacterial strain with a puncher with diameter of 5mm, inoculating to the centers of a medicine-containing plate and a blank control plate, covering a dish cover, placing in a constant temperature oven at 25 ℃ for culture, measuring the bacterial colony diameter by a crisscross method when the diameter of the blank control bacterial colony reaches more than 2/3 of the diameter of a culture dish, and calculating the inhibition rate of different treatments on hypha growth.
4. Data analysis: and (3) carrying out data statistical analysis by adopting DPS software, carrying out linear regression by taking the logarithmic value of the concentration of the bactericide as x and the corresponding value of the hypha growth inhibition rate as y to obtain a virulence regression equation and the virulence EC 50 value of the medicament on the target pathogen, and calculating a co-toxicity coefficient (CTC) according to the grand cloud Pei method.
In the above formula: ati—the measured virulence index of the mixture; s- -EC 50 of standard medicament in mg/L; m- -EC 50 of the mixture in mg/L.
TTI＝TIA×PA+TIB×PB
In the above formula: TTI- -theoretical toxicological index of the mixture; toxicity index of TI A - -A agents; the percentage content of the P A -A medicament in the mixture is expressed as percentage (%); toxicity index of TI B - -B agent; the percentage of the P B -B medicament in the mixture is expressed as percentage (%).
In the above formula: ctc—co-toxicity coefficient; ati—actual measured virulence index of the mixture; tti—the theoretical toxicity index of the mixture.
5. Measurement results
The synergy of the agents was evaluated based on the calculated co-toxicity coefficient (CTC), CTC.ltoreq.80 being antagonism, CTC.ltoreq.120 being additive, CTC.ltoreq.120 being synergy, the results being shown in tables 1-3.
TABLE 1 determination of indoor biological Activity of flutriafol and penflufen in combination with Rhizoctonia cerealis
| Medicament name and ratio | EC50(mg/L) | ATI | TTI | CTC |
| Flutriafol | 0.8316 | 100.0000 | -- | -- |
| Fluoxapyroxad | 4.4651 | 18.7252 | -- | -- |
| Flutriafol 1: penflufen 30 | 3.1497 | 26.5454 | 21.3470 | 124.3519 |
| Flutriafol 1: penflufen 20 | 2.5781 | 32.4309 | 22.5955 | 143.5283 |
| Flutriafol 1: fluoxapyroxad 10 | 0.9857 | 84.8230 | 26.1138 | 324.8200 |
| Flutriafol 1: fluoxapyroxad 5 | 1.9704 | 42.4330 | 32.2710 | 131.4895 |
| Flutriafol 1: fluoxapyroxad 1 | 0.4782 | 174.8432 | 59.3626 | 294.5341 |
| Flutriafol 5: fluoxapyroxad 1 | 0.6467 | 129.2872 | 86.4542 | 149.5441 |
| Flutriafol 10: fluoxapyroxad 1 | 0.4453 | 187.7611 | 92.6114 | 202.7408 |
| Flutriafol 20: fluoxapyroxad 1 | 0.1256 | 665.6847 | 96.1298 | 692.4855 |
| Flutriafol 30: fluoxapyroxad 1 | 0.4162 | 200.8890 | 97.3782 | 206.2976 |
| Flutriafol 40: fluoxapyroxad 1 | 0.6095 | 137.1780 | 98.0177 | 139.9523 |
As shown in Table 1, after the flutriafol and the penflufen are compounded, the mass ratio is 1-40: the co-toxicity coefficient of the rhizoctonia cerealis in the range of 30-1 is more than 120, and the synergistic effect is shown.
Table 2 determination of indoor bioactivity of tebuconazole and fluopicolide compounded rhizoctonia cerealis
As shown in table 2, after the flutriafol and the fluopicolide are compounded, the mass ratio is 1-15: the co-toxicity coefficient of the rhizoctonia cerealis in the range of 15-1 is more than 120, and the synergistic effect is shown.
In conclusion, when the flutriafol and the penflufen or the fluopicolide are compounded in the bactericidal composition disclosed by the invention, the synergistic effect is shown on inhibiting pathogenic bacteria of the sheath blight of wheat, the control effect on the sheath blight of wheat can be improved, the application dosage of pesticides can be reduced, and the control cost is reduced.
The foregoing is merely illustrative of the preferred embodiments of this invention and modifications, obvious to those skilled in the art, may be made without departing from the principles of this invention and are within the scope of this invention.
Claims (3)
1. The bactericidal composition for preventing and treating the wheat sheath blight is characterized by being prepared by compounding flutriafol and fluopicolide, wherein the mass ratio of flutriafol to fluopicolide is 1-15:15-1.
2. A bactericide, characterized by comprising the bactericidal composition for preventing and treating banded sclerotial blight of wheat as claimed in claim 1 and auxiliary components.
3. The bactericide according to claim 2, wherein the auxiliary component is one or more selected from the group consisting of dispersants, wetting agents, emulsifiers, solvents, binders, disintegrants, thickeners, fillers, antifreezes, stabilizers, preservatives, defoamers.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310011769.2A CN115943962B (en) | 2023-01-05 | 2023-01-05 | Sterilization composition for preventing and treating banded sclerotial blight of wheat |
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| CN202310011769.2A CN115943962B (en) | 2023-01-05 | 2023-01-05 | Sterilization composition for preventing and treating banded sclerotial blight of wheat |
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| CN115943962B true CN115943962B (en) | 2024-06-25 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1494377A (en) * | 2001-03-08 | 2004-05-05 | �ݶ�ũ�ƹɷ�����˾ | Fungicide compositions based on pyridylethylbenzamide and propamocarb derivative |
| CN1585603A (en) * | 2001-11-14 | 2005-02-23 | 拜尔农科股份有限公司 | Fungicidal composition based on a pyridylmethylbenzamide derivative and a valinamide derivative |
| CN1917760A (en) * | 2004-02-12 | 2007-02-21 | 拜尔农科股份有限公司 | Fungicidal composition comprising a pyridylethylbenzamide derivative and a compound capable of inhibiting the ergosterol biosynthesis |
| WO2007128541A2 (en) * | 2006-05-08 | 2007-11-15 | Syngenta Participations Ag | Pesticidal combinations comprising flutriafol |
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| CN104621123B (en) * | 2013-11-15 | 2016-07-27 | 南京华洲药业有限公司 | A kind of bactericidal composition containing prothioconazoles and fluopicolide and application thereof |
| CN104041505A (en) * | 2014-03-01 | 2014-09-17 | 海南正业中农高科股份有限公司 | Fluopicolide-containing bactericidal composition |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1494377A (en) * | 2001-03-08 | 2004-05-05 | �ݶ�ũ�ƹɷ�����˾ | Fungicide compositions based on pyridylethylbenzamide and propamocarb derivative |
| CN1585603A (en) * | 2001-11-14 | 2005-02-23 | 拜尔农科股份有限公司 | Fungicidal composition based on a pyridylmethylbenzamide derivative and a valinamide derivative |
| CN1917760A (en) * | 2004-02-12 | 2007-02-21 | 拜尔农科股份有限公司 | Fungicidal composition comprising a pyridylethylbenzamide derivative and a compound capable of inhibiting the ergosterol biosynthesis |
| WO2007128541A2 (en) * | 2006-05-08 | 2007-11-15 | Syngenta Participations Ag | Pesticidal combinations comprising flutriafol |
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