CN115933153A - Illumination system and microscope - Google Patents

Abstract

The invention relates to an illumination system and a microscope. The illuminating system comprises a standard light source, an illuminating light source, a light splitting element and a lens assembly, wherein the lens assembly is arranged on the light emitting side of the light splitting element, the light splitting element can receive at least part of light emitted by the standard light source and the illuminating light source and can emit at least part of light emitted by the standard light source and the illuminating light source towards the lens assembly, and the lens assembly is used for emitting the light emitted by the light splitting element towards a sample. The lighting system has the advantages of simple switching operation of the lighting source and the standard light source and convenience in use.

Description

Illumination system and microscope

Technical Field

The invention relates to the technical field of microscopic imaging, in particular to an illumination system and a microscope.

Background

At present, when a microstructure such as a biological tissue is inspected in the biomedical field, it is generally required to acquire an image of the microstructure and spectral information of a specific waveband so as to acquire changes of the microstructure in terms of content, structural morphology and the like. The microscope is used for obtaining the image of the microstructure, and usually an illumination light source is used for illumination, and the standard light source is used for spectrum calibration for obtaining the specific waveband spectrum information of the microstructure. However, in the related art, the structure of the illumination light source and the standard light source of the microscope is complicated, and the use is inconvenient.

Disclosure of Invention

Therefore, it is necessary to provide an illumination system and a microscope for solving the problems of complicated structure and inconvenient use of the illumination light source and the standard light source of the microscope.

The utility model provides an illumination system, includes standard light source, beam splitting component and lens subassembly, the lens subassembly is located beam splitting component's light-emitting side, beam splitting component can receive standard light source and at least some light of light source outgoing, and can with standard light source and at least some light of light source outgoing towards the lens subassembly outgoing, the lens subassembly is used for with the light of beam splitting component outgoing is towards the sample outgoing.

In one embodiment, the light emitted from the standard light source and the light emitted from the illumination light source can enter the light splitting element from different sides of the light splitting element, and the light splitting element can reflect the light emitted from the standard light source and transmit the light emitted from the illumination light source.

In one embodiment, the light splitting element has a light splitting surface, the light splitting surface is inclined to the optical axis of the lens assembly, the light emitted by the illumination light source enters the light splitting element from a side of the light splitting surface opposite to the lens assembly, the light emitted by the standard light source enters the light splitting element from a side of the light splitting surface facing the lens assembly, and the light splitting surface can transmit the light emitted by the illumination light source and reflect the light emitted by the standard light source.

In one embodiment, the light splitting element includes a first right-angle prism and a second right-angle prism, inclined surfaces of the first right-angle prism and the second right-angle prism are abutted to form the light splitting surface, one side surface of the first right-angle prism faces the lens assembly and is perpendicular to an optical axis of the lens assembly, light emitted from the standard light source enters the light splitting element from the other side surface of the first right-angle prism, and light emitted from the illumination light source enters the light splitting element from a side surface of the second right-angle prism.

In one embodiment, the light emitted from the standard light source and the incident direction of the light emitted from the illumination light source on the light splitting element are perpendicular to each other.

In one embodiment, the lens assembly includes a plurality of coaxially arranged convex lenses.

In one embodiment, the lighting system further includes a first controller, a light guide element, and a light source interface, two ends of the light guide element are respectively connected to the first controller and the light source interface, a light outlet of the light source interface is opposite to the light splitting element, and the first controller is configured to couple light emitted from the standard light source into the light guide element.

In one embodiment, the illumination source is fixed on a side of the light splitting element, which faces away from the lens assembly, and a light emitting surface of the illumination source faces the light splitting element.

In one embodiment, the lighting system further includes a second controller, the lighting source has a positive electrode and a negative electrode, and the two electrodes of the second controller are electrically connected to the positive electrode and the negative electrode of the lighting source respectively to control the on or off of the lighting source and/or control the output power of the lighting source.

A microscope comprising a stage for holding a sample and an illumination system as described in any preceding embodiment for illuminating the sample.

Above-mentioned lighting system, the cooperation of beam splitting component and lens subassembly can be with the light conduction of standard light source and light source outgoing to the sample on in order to throw light on the sample, when the image of sample is acquireed to needs, only need close standard light source to open light source, make light source outgoing can throw light on the sample through beam splitting component and lens subassembly, and in the same way, when the spectral information of sample is acquireed to needs, only need close light source and open standard light source can. Therefore, the lighting system has the advantages that the lighting source and the standard light source are integrated in one system, the structure is simple, the light source does not need to be detached, replaced or shifted when the lighting source and the standard light source are switched for use, and the use is more convenient.

Drawings

Fig. 1 is a schematic diagram of an illumination system in some embodiments.

Reference numerals:

10. an illumination system; 101. a lighting module; 102. an illumination light source; 1022. a positive electrode; 1024. a negative electrode; 103. a light-splitting element; 1032. a light splitting surface; 1034. a first right-angle prism; 1036. a second right-angle prism; 104. a lens assembly; 1042. a convex lens; 105. a first controller; 106. a light guide element; 107. a light source interface; 108. a second controller; 109. a control module; 201. an object stage.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, embodiments accompanying figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "above," and "over" a second feature may be directly on or obliquely above the second feature, or simply mean that the first feature is at a higher level than the second feature. A first feature "under," "beneath," and "under" a second feature may be directly under or obliquely under the second feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. As used herein, the terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like are for purposes of illustration only and do not denote a single embodiment.

In the related art, the light source used by the microscope to acquire the image of the sample and the spectral information of the sample is different, for example, an illumination light source is used to acquire the image of the sample, and a standard light source is used to acquire the spectral information of the sample. In the related art, the illumination light source and the standard light source are installed separately, and when the image of the sample and the spectral information of the sample need to be obtained separately, it is usually necessary to switch the illumination light source and the standard light source, for example, to detach one of the light sources and replace the other light source, or to transfer the other light source to the sample through the transfer of a mechanical structure, which results in a complicated structure of the illumination system and inconvenience in use.

To solve the above problems, the present application provides an illumination system and a microscope.

Referring to fig. 1, fig. 1 is a schematic structural diagram of an illumination system 10 according to some embodiments. The illumination system 10 may be used in a microscope, and the illumination system 10 may be capable of emitting light to illuminate a sample of the microscope to facilitate observation of the sample. Of course, the illumination system 10 may be used in any suitable device that requires illumination of a sample, which may be a microstructure such as a biological tissue, to facilitate viewing of the sample. For example, the illumination system 10 may be applied to a microscope for illuminating a sample by emitting light rays of different wavelength bands to obtain an image or spectral information of the sample, so as to observe changes in the content, structural morphology and the like of tissue composition information of a biological tissue caused by a disease.

Further, in some embodiments, the illumination system 10 includes a standard light source (not shown), an illumination light source 102, a light splitting element 103, and a lens assembly 104, where the lens assembly 104 is disposed on a light emitting side of the light splitting element 103, and light emitted from the standard light source and the illumination light source 102 can be incident on the light splitting element 103. The light splitting element 103 can receive the light emitted from the standard light source and the illumination light source 102, and can emit the light emitted from the standard light source and the illumination light source 102 toward the lens assembly 104. The lens assembly 104 is used for emitting the light emitted from the light splitting element 103 toward the sample to illuminate the sample.

It will be appreciated that the illumination system 10, the beam splitting element 103 and the lens assembly 104 are adapted to direct light from both the standard light source and the illumination source 102 onto the sample to illuminate the sample. When an image of a sample needs to be acquired, only the standard light source needs to be turned off, and the illumination light source 102 is turned on, so that the illumination light source 102 emits light, and the light emitted by the illumination light source 102 can illuminate the sample through the light splitting element 103 and the lens assembly 104. Similarly, when the spectrum information of the sample needs to be acquired, only the illumination light source 102 needs to be turned off and the standard light source needs to be turned on, and the light emitted by the standard light source can illuminate the sample through the light splitting element 103 and the lens assembly 104. Therefore, in the lighting system 10, the lighting source 102 and the standard light source are integrated into one system, the structure is simple, and when the lighting source 102 and the standard light source are switched for use, the lighting source 102 and the standard light source are only required to be controlled to be turned on or turned off, the light source does not need to be detached, replaced or transferred, and the lighting system 10 is more convenient to use.

It should be noted that, in the present application, turning on a certain light source may be understood as turning on the light source through a switch button or software circuit control, or exposing a light outlet of the light source through the cooperation of a mechanical structure, so that the light source can emit light, and turning off a certain light source may be understood as turning off the light source through a switch button or software circuit control, or blocking a light outlet of the light source through the cooperation of a mechanical structure, so that the light source cannot emit light.

In the present application, the wavelength and type of the light emitted from the illumination light source 102 are not limited as long as the illumination of the sample can be satisfied, so as to obtain an image of the sample. For example, in some embodiments, the light emitting wavelength of the illumination source 102 is between 400nm and 1000nm, and the light emitting wavelength of the illumination source 102 covers a larger wavelength range, which is beneficial to more comprehensively acquiring an image of a sample, and improving the accuracy of observing or detecting the sample. Specifically, the illumination light source 102 includes, but is not limited to, a light emitting element such as a light emitting diode, a halogen lamp, or the like. Of course, the wavelength and type of the light emitted from the illumination source 102 may be selected from other options, and may be specifically designed according to the illumination requirement of the sample, as long as the illumination requirement of the sample can be satisfied, and the wavelength range and type of the light emitted from the illumination source 102 are not specifically limited in the present application.

It will be appreciated that when the sample is illuminated with the illumination source 102, the microscope may also be configured with a sensor that is adapted to the wavelength range of the light emitted by the illumination source 102, the sensor being configured to receive light reflected from the sample to obtain an image of the sample.

In this application, the wavelength and type of the light emitted from the standard light source are not limited, as long as the spectrum calibration requirement of the sample can be met, so as to obtain the spectrum information of the sample. For example, in some embodiments, the wavelength of the output light of the standard light source may be between 400nm and 1000nm, and of course, the wavelength of the output light of the standard light source may also have a larger range, for example, between 250nm and 1700nm, so as to obtain a larger range of spectral information of the sample, and improve the detection accuracy. The standard light source includes but is not limited to light emitting elements such as mercury lamps, mercury argon lamps, xenon lamps, cadmium lamps, etc., and can be designed according to the requirements of the detected spectral range.

It is understood that when the sample is illuminated by the standard light source, one or more elements such as mercury (Hg), neon (Ne), argon (Ar), cadmium (Cd), cesium (Cs), helium (He), and thallium (Tl) may be added to the sample, and the spectral information of the sample may be obtained by different reactions of the elements to light rays in different spectral ranges. When the sample is illuminated by a standard light source, the microscope may further be provided with a spectrometer or other device capable of receiving spectral information, the spectrometer being configured to receive light reflected or excited by the sample to obtain spectral information of the sample.

Further, in some embodiments, the light emitted from the standard light source and the illumination light source 102 can enter the light splitting element 103 from different sides of the light splitting element 103, and the light splitting element 103 can reflect at least a portion of the light emitted from the standard light source and transmit at least a portion of the light emitted from the illumination light source 102, so that at least a portion of the light emitted from the standard light source and the illumination light source 102 can be emitted toward the lens assembly 104.

For example, in some embodiments, the light splitting element 103 has a light splitting surface 1032, the light splitting surface 1032 is inclined to the optical axis of the lens component 104, the light emitted from the illumination source 102 enters the light splitting element 103 from a side of the light splitting surface 1032 facing away from the lens component 104, the light emitted from the standard light source enters the light splitting element 103 from a side of the light splitting surface 1032 facing toward the lens component 104, and the light splitting surface 1032 can transmit the light emitted from the illumination source 102 and reflect the light emitted from the standard light source. The light emitted from the illumination source 102 is transmitted through the light splitting surface 1032 and then emitted to the lens assembly 104, and the light emitted from the standard light source is reflected by the light splitting surface 1032 toward the lens assembly 104. It should be understood that, in the present application, taking the plane of the light splitting surface 1032 as an example, the plane divides two side spaces, and the space of the lens assembly 104 on one side thereof, the elements located in the same side space with the lens assembly 104 can be regarded as being located on the side of the light splitting surface 1032 facing the lens assembly 104, and the elements located in the different side spaces with the lens assembly 104 can be regarded as being located on the side of the light splitting surface 1032 facing away from the lens assembly 104.

In some embodiments, the light splitting element 103 includes a first right angle prism 1034 and a second right angle prism 1036, the inclined surfaces of the first right angle prism 1034 abut to form the light splitting surface 1032, e.g., the first right angle prism 1034 is glued to the second right angle prism 1036 at the inclined surfaces. One side surface of the first right-angle prism 1034 faces the lens assembly 104 and is perpendicular to the optical axis of the lens assembly 104, the other side surface is parallel to the optical axis of the lens assembly 104, and the light emitted from the standard light source enters the light splitting element 103 from the other side surface of the first right-angle prism 1034. One side surface of the second right-angle prism 1036 is perpendicular to the optical axis of the lens assembly 104, and the other side surface is parallel to the optical axis of the lens assembly 104, and the light emitted from the illumination light source 102 enters the light splitting element 103 from the side surface of the second right-angle prism 1036 perpendicular to the optical axis of the lens assembly 104. In the present application, a surface of the right-angle prism corresponding to a hypotenuse of the right-angle triangle of the cross section is referred to as an inclined surface of the right-angle prism, and two surfaces of the right-angle prism corresponding to two legs of the right-angle triangle of the cross section are both referred to as side surfaces of the right-angle prism.

The specific arrangement of the light splitting element 103 is not limited as long as the light splitting element can reflect the light emitted from the standard light source and transmit the light emitted from the illumination light source 102, so that the light incident on the light splitting element 103 from different sides can be emitted toward the lens assembly 104. For example, in some embodiments, the light splitting element 103 is provided with a transflective film at the intersection of the first rectangular prism 1034 and the second rectangular prism 1036, i.e., at the light splitting plane 1032. The transflective film can transmit a part of the light emitted from the standard light source and reflect a part of the light emitted from the standard light source, and the transflective film can transmit a part of the light emitted from the illumination light source 102 and reflect a part of the light emitted from the illumination light source 102. For example, the transflective film can reflect 40%, 50% or 60% of the light emitted from the standard light source, and can transmit 40%, 50% or 60% of the light emitted from the illumination light source 102, as long as the illumination requirement of the sample can be satisfied when the standard light source or the illumination light source 102 emits light. In other embodiments, the light splitting surface 1032 may further be provided with a selective transmission film layer, and the material of the selective transmission film layer may be designed according to the emergent light of the standard light source and the illumination light source 102, so as to reflect the light emitted from the standard light source and the light emitted from the illumination light source 102 to the maximum extent, for example, more than 60% of the light emitted from the standard light source can be reflected, more than 60% of the light emitted from the illumination light source 102 can be transmitted, and the utilization efficiency of the light can be improved. The design relationship among the standard light source, the illumination light source 102, and the light splitting element 103, and the specific arrangement of the light splitting element 103 are not limited, for example, the light splitting element 103 may also be one or more light splitting prisms, as long as the light splitting element 103 can emit the light beams emitted from the standard light source and the illumination light source 102 incident from different positions toward the lens assembly 104 to illuminate the sample, and the illumination requirement of the sample is met, and the arrangement of the light splitting element 103 is not specifically limited in this application.

In the embodiment shown in fig. 1, the incident directions of the light emitted from the standard light source and the light emitted from the illumination light source 102 on the light splitting element 103 are perpendicular to each other, in other words, the central light of the light beam incident from the standard light source to the light splitting element 103 is perpendicular to the central light of the light beam incident from the illumination light source 102 to the light splitting element 103, and the light splitting surface 1032 can form an included angle of 45 degrees with the optical axis of the lens assembly 104, so that the light emitted from the standard light source and the light emitted from the illumination light source 102 can be emitted toward the lens assembly 104 effectively, and the structural design and layout of the illumination system 10 are simpler. In other embodiments, the angle of the light splitting surface 1032 with respect to the optical axis of the lens assembly 104 and the incident angle of the light emitted from the standard light source and the illumination light source 102 on the light splitting element 103 may also have other designs, as long as the light splitting element 103 can emit the light emitted from the standard light source and the illumination light source 102 toward the lens assembly 104, which is not described herein again.

The lens assembly 104 may include a plurality of lenses with focal power, and the lens assembly 104 has a collimating and/or light-homogenizing effect due to the cooperation of the plurality of lenses with focal power, so that the light emitted from the standard light source and the light emitted from the illumination light source 102 can be more uniform and illuminate the sample at a good incident angle, thereby improving the illumination effect and satisfying the requirement for observing the sample. For example, the lens assembly 104 may include a plurality of convex lenses 1042, the plurality of convex lenses 1042 are coaxially arranged, and an axis common to the plurality of convex lenses 1042 may be understood as an optical axis of the lens assembly 104. Of course, the type and number of the lenses in the lens assembly 104 may also have other designs, which may be specifically designed according to the light emitting condition of the light splitting element 103 and the illumination requirement of the sample, and the present application does not specifically limit the arrangement of the lens assembly 104.

In some embodiments, the lighting system 10 further includes a first controller 105, a light guiding element 106, and a light source interface 107, the light guiding element 106 may be an optical fiber, two ends of the light guiding element 106 are respectively connected to the first controller 105 and the light source interface 107, the first controller 105 may be a controller of a standard light source, and the first controller 105 may be configured to control the standard light source to be turned on and off. The standard light source can be disposed in the first controller 105 or connected to the first controller 105, and the first controller 105 is further configured to couple light emitted from the standard light source into the light guide element 106. The light source interface 107 can be in butt joint with the fixing structure of the light splitting element 103, a light outlet of the light source interface 107 is opposite to the light splitting element 103, and light emitted by the standard light source sequentially passes through the light guide element 106 and the light source interface 107 and then is emitted towards the light splitting element 103.

In some embodiments, the illumination source 102 is fixed on a side of the light splitting element 103 opposite to the lens component 104, and a light emitting surface of the illumination source 102 faces the light splitting element 103. In some embodiments, the lighting system 10 further includes a second controller 108, and the second controller 108 may be a controller of the lighting source 102 for controlling the lighting source 102 to be turned on and off, and when the lighting source 102 is turned on, the second controller 108 may also control the output power of the lighting source 102 to meet different lighting requirements. For example, the illumination source 102 may have a positive pole 1022 and a negative pole 1024, and the two poles of the second controller 108 are electrically connected to the positive pole 1022 and the negative pole 1024 of the illumination source 102, respectively, to control the illumination source 102.

It is understood that in the above embodiments, the illumination light source 102, the light splitting element 103 and the lens assembly 104 may be integrated into one illumination module 101, for example, the illumination light source 102, the light splitting element 103 and the lens assembly 104 are fixed to each other by a fixing structure, the first controller 105 and the second controller 108 are disposed outside the illumination module 101, the first controller 105 is interfaced with the illumination module 101 through the light guiding element 106 and the light source interface 107, and the second controller 108 is electrically connected with the illumination light source 102 through a wire. By such arrangement, the lighting system 10 has high structural integration level, simple structural arrangement and wiring, high space utilization rate, and convenient control and operation of the lighting source 102 and the standard light source.

Further, in some embodiments, the lighting system 10 may further include a control module 109, the control module 109 may include an operation panel or a display panel, and the control module 109 may electrically connect the first controller 105 and the second controller 108, so as to control the first controller 105 and the second controller 108 through software or manual operation of the control panel, and thus control the lighting source 102 and the standard light source to be turned on or off.

The present application further provides a microscope (not shown) comprising a stage 201 and an illumination system 10 as described in any of the above embodiments, wherein the stage 201 is used for fixing a sample, and the light-emitting side of the lens assembly 104 can be opposite to the stage 201 so as to facilitate the illumination system 10 to illuminate the sample. In some embodiments, the microscope may further include other components such as a spectrometer that may obtain spectral information of the sample when the standard light source emits light, and a sensor that may obtain an image of the sample when the illumination light source 102 emits light.

Although not shown, it should be understood by those skilled in the art that the microscope and illumination system 10 of the present application may further include any other suitable elements to achieve the corresponding functions, and the above elements may be replaced by other elements to achieve the same functions, as long as the switching step between the illumination light source 102 and the standard light source can be simplified, and will not be described herein again.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (10)

1. The lighting system is characterized by comprising a standard light source, a lighting source, a light splitting element and a lens assembly, wherein the lens assembly is arranged on the light emitting side of the light splitting element, the light splitting element can receive at least part of light emitted by the standard light source and the lighting source and can emit at least part of light emitted by the standard light source and the lighting source to the lens assembly, and the lens assembly is used for emitting the light emitted by the light splitting element to a sample.

2. The illumination system of claim 1, wherein the light emitted from the standard light source and the light emitted from the illumination light source can enter the light splitting element from different sides of the light splitting element, and the light splitting element can reflect the light emitted from the standard light source and transmit the light emitted from the illumination light source.

3. The illumination system according to claim 2, wherein the light splitting element has a light splitting surface, the light splitting surface is inclined to the optical axis of the lens assembly, the light emitted from the illumination light source enters the light splitting element from a side of the light splitting surface facing away from the lens assembly, the light emitted from the standard light source enters the light splitting element from a side of the light splitting surface facing toward the lens assembly, and the light splitting surface is capable of transmitting the light emitted from the illumination light source and reflecting the light emitted from the standard light source.

4. The illumination system according to claim 3, wherein the light splitting element includes a first right-angle prism and a second right-angle prism, inclined surfaces of the first right-angle prism and the second right-angle prism are abutted to form the light splitting surface, one side surface of the first right-angle prism faces the lens assembly and is perpendicular to an optical axis of the lens assembly, the light emitted from the standard light source enters the light splitting element from the other side surface of the first right-angle prism, and the light emitted from the illumination light source enters the light splitting element from the side surface of the second right-angle prism.

5. The illumination system of claim 2, wherein the light emitted from the standard light source and the light emitted from the illumination light source are incident on the light splitting element in directions perpendicular to each other.

6. The illumination system of claim 1, wherein the lens assembly comprises a plurality of coaxially disposed convex lenses.

7. The illumination system according to claim 1, further comprising a first controller, a light guide element, and a light source interface, wherein two ends of the light guide element are respectively connected to the first controller and the light source interface, a light outlet of the light source interface is opposite to the light splitting element, and the first controller is configured to couple light emitted from the standard light source into the light guide element.

8. The illumination system of claim 1, wherein the illumination source is fixed on a side of the light splitting element facing away from the lens assembly, and a light emitting surface of the illumination source faces the light splitting element.

9. The lighting system according to claim 1, further comprising a second controller, wherein the lighting source has a positive electrode and a negative electrode, and the two electrodes of the second controller are electrically connected to the positive electrode and the negative electrode of the lighting source respectively to control the on/off of the lighting source and/or to control the output power of the lighting source.

10. A microscope comprising a stage for holding a sample and an illumination system according to any one of claims 1 to 9 for illuminating the sample.

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