CN115572675A - Matrix temperature control auxiliary heating device for PCR instrument and PCR instrument - Google Patents
Matrix temperature control auxiliary heating device for PCR instrument and PCR instrument Download PDFInfo
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- CN115572675A CN115572675A CN202211425071.7A CN202211425071A CN115572675A CN 115572675 A CN115572675 A CN 115572675A CN 202211425071 A CN202211425071 A CN 202211425071A CN 115572675 A CN115572675 A CN 115572675A
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- 238000010438 heat treatment Methods 0.000 title claims abstract description 332
- 239000011159 matrix material Substances 0.000 title claims abstract description 53
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- 238000009434 installation Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 10
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- 230000004543 DNA replication Effects 0.000 description 5
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- 238000000338 in vitro Methods 0.000 description 4
- 230000000295 complement effect Effects 0.000 description 3
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- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000004153 renaturation Methods 0.000 description 2
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- 239000002994 raw material Substances 0.000 description 1
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Abstract
The invention discloses a matrix temperature control auxiliary heating device for a PCR instrument and the PCR instrument, wherein the matrix temperature control auxiliary heating device for the PCR instrument comprises a temperature control module, an auxiliary heating module, a control module and a temperature sensor, wherein an accommodating groove on the temperature control module is used for accommodating a sample tube, so that the temperature of the sample tube can be adjusted in the accommodating groove, the control module is used for controlling the temperature of the temperature control module, and further the control module can control the temperature of a cylinder; offer the through-hole that is the matrix and distribute on the auxiliary heating module, sample pipe can run through the through-hole and stretch into the holding tank when being used for the installation of the matrix control by temperature change auxiliary heating device of PCR appearance, when the heater heating, can heat the cylinder through heat transfer, can play auxiliary heating's purpose, thereby make the inside temperature of a plurality of cylinders more even, and then can make a plurality of sample pipes better at the temperature homogeneity of same moment, finally make sample pipe testing result's accuracy nature better.
Description
Technical Field
The invention relates to the technical field of biological detection, in particular to a matrix temperature control auxiliary heating device for a PCR instrument and the PCR instrument.
Background
The existing PCR analyzers can be roughly classified into two types from the viewpoint of module composition, one type is composed of only a temperature control device, and the other type is composed of a temperature control device and an optical detection device. The temperature control device has the main function of providing a proper temperature environment for the in-vitro DNA replication of a sample to be tested, and particularly, the in-vitro DNA replication process of the sample to be tested comprises the steps that DNA is denatured at high temperature, a double strand is opened to become a single strand, the DNA single strand is combined with a primer in a sample tube to be tested according to the base complementary pairing principle at low temperature, and the DNA is replicated by means of polymerase at the optimal reaction temperature of the polymerase. The temperature of the sample to be detected needs to be accurately controlled in the execution process of the three steps, otherwise, the research result generates deviation, and even the detection fails.
When the existing PCR instrument is used for heating or cooling a sample to be detected, a plurality of samples to be detected have large temperature difference in the actual running process, and further the temperature uniformity among the samples to be detected at the same moment is poor. Therefore, how to improve the temperature uniformity among a plurality of samples to be detected in the detection process is an urgent problem to be solved.
Disclosure of Invention
The invention aims to provide a matrix temperature control auxiliary heating device for a PCR instrument and the PCR instrument, and solves the problem of poor temperature uniformity.
In order to achieve the purpose, the invention provides a technical scheme that:
a matrix temperature control auxiliary heating device for a PCR instrument comprises:
the temperature control module comprises a plurality of columns, and an accommodating groove for accommodating the sample tube is formed in each column so that the sample tube can be adjusted in the accommodating groove;
the auxiliary heating module is connected with the temperature control module, a plurality of through holes distributed in a matrix form are formed in the auxiliary heating module, the cylinder penetrates through the through holes, a plurality of transverse heating wires and a plurality of longitudinal heating wires are further arranged on the auxiliary heating module, the transverse heating wires are located on one row of the through holes, one transverse heating wire corresponds to one row of the through holes, the longitudinal heating wires are located on one row of the through holes, one longitudinal heating wire corresponds to one row of the through holes, the transverse heating wires and the longitudinal heating wires are arranged in a staggered mode to form intersection points, heaters are arranged at least on part of the intersection points, and the transverse heating wires and the longitudinal heating wires at the corresponding intersection points are electrically connected with the heaters;
the control module controls the plurality of transverse heating wires and the plurality of longitudinal heating wires to be sequentially switched on or switched off, and when the transverse heating wires, the longitudinal heating wires and the corresponding heaters form a loop so that the corresponding heaters heat the corresponding columns;
the temperature sensor is used for detecting the temperature inside the cylinder, and the temperature sensor is used for transmitting a detected signal to the control module, and the control module controls the temperature of the heater.
Optionally, when one of the transverse heating wires is electrified, the other transverse heating wires are not electrified, and the longitudinal heating wires are sequentially electrified, so that the heaters heat the cylinders one by one.
Optionally, when one of the transverse heating lines is powered on, the rest of the transverse heating lines are not powered on, and the plurality of longitudinal heating lines are powered on, so that the heaters heat the cylinders in groups.
Optionally, the plurality of transverse heating wires are energized for different durations, or the plurality of longitudinal heating wires are energized for different durations, so that the heating power is different between the heaters.
Optionally, the auxiliary heating module is detachably connected to the temperature control module.
Optionally, the adjacent columns are connected through a bearing table, and the bearing table is used for supporting the auxiliary heating module.
Optionally, the height of the top end of the column is higher than the height of the bearing table.
Optionally, the bearing table is provided with lightening holes.
Optionally, the through holes on the auxiliary heating module are distributed in a matrix form in an m × n manner, where m is not less than 2, and n is not less than 2.
The invention also provides a technical scheme that:
a PCR instrument, comprising: the matrix temperature control auxiliary heating device for the PCR instrument is positioned above the heat dissipation device;
the heater of the matrix temperature control auxiliary heating device for the PCR instrument has different heating powers.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention discloses a matrix temperature control auxiliary heating device for a PCR instrument, which comprises a temperature control module, an auxiliary heating module, a control module and a temperature sensor, wherein an accommodating groove on the temperature control module is used for accommodating a sample tube, so that the temperature of the sample tube can be adjusted in the accommodating groove, through holes distributed in a matrix form are formed in the auxiliary heating module, the sample tube can penetrate through the through holes and extend into the accommodating groove when the matrix temperature control auxiliary heating device for the PCR instrument is installed, a plurality of transverse heating wires and a plurality of longitudinal heating wires are also arranged on the auxiliary heating module, the transverse heating wires and the longitudinal heating wires are arranged in a criss-cross mode to form a plurality of intersection points, heaters are arranged at least at part of intersection points, the transverse heating wires and the longitudinal heating wires at the corresponding intersection points are electrically connected with the heaters, the heaters are equivalent to the heaters arranged at the periphery of the through holes, and when the auxiliary heating module is connected with the temperature control module, each heater just corresponds to one cylinder and is arranged beside the cylinder; the control module controls the plurality of transverse heating wires and the plurality of longitudinal heating wires to be sequentially switched on or off, and when the transverse heating wires, the longitudinal heating wires and the corresponding heaters form a loop so that the corresponding heaters heat the cylinder; when the heater is used for heating, the column body can be heated through heat transfer, and then the heat is transferred to the sample tube in the column body, so that the purpose of auxiliary heating of the sample tube is achieved, the sample tube can be subjected to temperature compensation, and the problem of poor temperature uniformity among a plurality of sample tubes is solved; the temperature sensor is used for detecting the temperature inside the column body, namely the temperature of the sample tube can be detected, then the temperature sensor can transmit a detected signal to the control module, and the temperature of the heater is controlled through the control module; when the temperature control module heats the to-be-measured samples in the sample tubes, the temperature sensor senses that the internal temperature of the partial cylinder does not reach the preset temperature, the control module controls the transverse heating wires and the longitudinal heating wires of the auxiliary heating module to be sequentially switched on and off, so that the corresponding heater heats the corresponding cylinder, the auxiliary heating module completes the auxiliary heating of the sample tubes, and the temperature uniformity performance between the sample tubes is improved.
2. When the transverse heating wire and the longitudinal heating wire of the matrix temperature control auxiliary heating device for the PCR instrument are communicated with the heater, the heater is started; after at least one of the transverse heating wire and the longitudinal heating wire is disconnected with the heater, the heater is closed, so that the control module can control the opening and closing of the heater by controlling the transverse heating wire and the longitudinal heating wire, the use is convenient, and the arrangement modes of the transverse heating wire and the longitudinal heating wire are tidy.
3. The adjacent columns in the matrix temperature control auxiliary heating device for the PCR instrument are connected through the bearing table, the bearing table is used for supporting the auxiliary heating module, the height of the bearing table determines the position of the heater relative to the sample tube, and the position of the bearing table can be changed according to actual use requirements.
4. The bearing table of the matrix temperature control auxiliary heating device for the PCR instrument is provided with lightening holes, and the weight of the whole temperature control module can be lightened due to the arrangement of the lightening holes.
5. The control module of the matrix temperature control auxiliary heating device for the PCR instrument is also used for controlling the temperature of the temperature control module, so that the control module can control the temperature of the cylinder bodies, and can achieve the main heating purpose.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
FIG. 1 is a schematic structural diagram of a matrix temperature-controlled auxiliary heating device for a PCR instrument according to the present invention;
FIG. 2 is a first structural schematic diagram of an auxiliary heating module of the matrix temperature control auxiliary heating device for a PCR instrument of the present invention;
FIG. 3 is a schematic structural diagram of a first view angle of a temperature control module of the matrix temperature control auxiliary heating device for a PCR instrument according to the present invention;
FIG. 4 is a schematic structural view of a sample tube;
FIG. 5 is a schematic circuit diagram of an auxiliary heating module of the matrix temperature control auxiliary heating device for a PCR instrument according to the present invention;
FIG. 6 is a schematic structural diagram of a second view angle of the temperature control module of the matrix temperature control auxiliary heating apparatus for PCR instrument according to the present invention;
FIG. 7 is a second structural diagram of an auxiliary heating module of the matrix temperature control auxiliary heating device for a PCR instrument of the present invention;
FIG. 8 is a schematic view of the PCR apparatus of the present invention.
In the figure:
1. a temperature control module; 11. a cylinder; 111. accommodating grooves; 12. a bearing table; 121. lightening holes; 13. mounting grooves; 2. an auxiliary heating module; 21. a through hole; 22. a heater; 23. a transverse heating wire; 24. a longitudinal heating wire; 3. a sample tube; 4. a heat sink is provided.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the PCR instrument in the prior art, when a sample to be tested is subjected to in vitro DNA replication, DNA is denatured at a high temperature (about 95 ℃ (also referred to as a denaturation temperature), a double strand is opened to become a single strand, and at a low temperature (about 60 ℃ (also referred to as a renaturation temperature), the single strand of DNA is combined with a primer in the sample to be tested according to the base complementary pairing principle, and at an optimal reaction temperature (about 72 ℃ (also referred to as an extension temperature), the DNA is replicated by using polymerase, and in this process, a temperature control module can control among the denaturation temperature, the renaturation temperature, and the extension temperature, so that the sample to be tested can be subjected to in vitro DNA replication.
Therefore this embodiment provides a matrix control by temperature change auxiliary heating device for PCR appearance, has solved the problem that the temperature homogeneity is poor between the sample that awaits measuring in the PCR experimentation.
Referring to fig. 1, the embodiment of the present disclosure provides a matrix temperature control auxiliary heating device for a PCR instrument, which includes a temperature control module 1, an auxiliary heating module 2, a control module and a temperature sensor. As shown in fig. 1, the temperature control module 1 includes a plurality of columns 11, and a receiving groove 111 for receiving the sample tube 3 is formed in each column 11, so that the sample tube 3 can be temperature-controlled in the receiving groove 111; as shown in fig. 1 and 2, the auxiliary heating module 2 is connected to the temperature control module 1, specifically, a plurality of through holes 21 distributed in a matrix are formed in the auxiliary heating module 2, the pillars 11 penetrate through the through holes 21, so that the auxiliary heating module 2 is connected to the temperature control module 1, a plurality of transverse heating wires 23 and a plurality of longitudinal heating wires 24 are further disposed on the auxiliary heating module 2, the transverse heating wires 23 are disposed on one side of a row of through holes 21, one transverse heating wire 23 corresponds to one row of through holes 21, the longitudinal heating wire 24 is disposed on one side of one row of through holes 21, one longitudinal heating wire 24 corresponds to one row of through holes 21, the transverse heating wires 23 and the longitudinal heating wires 24 are arranged in a staggered manner to form intersections, at least a part of the intersections are provided with heaters 22, the transverse heating wires 23 and the longitudinal heating wires 24 at the corresponding intersections are electrically connected to the heaters 22, which are equivalent to the heaters 22 disposed around the through holes 21, when the auxiliary heating module 2 is connected to the temperature control module 1, each heater 22 corresponds to one pillar 11 and is disposed just beside the pillar 11; the control module controls the plurality of transverse heating wires 23 and the plurality of longitudinal heating wires 24 to be sequentially powered on or powered off, and when the plurality of transverse heating wires 23 and the plurality of longitudinal heating wires 24 are powered on, the transverse heating wires 23, the longitudinal heating wires 24 and the corresponding heaters 22 form a loop, so that the corresponding heaters 22 heat the corresponding columns 11.
It should be noted that the intersection point formed by the staggered arrangement of the transverse heating wires 23 and the longitudinal heating wires 24 is only a virtual intersection point where the transverse heating wires 23 are not connected with the longitudinal heating wires 24, and is not a circuit welding point where the transverse heating wires 23 are electrically connected with the longitudinal heating wires 24, the heater 22 is arranged at the virtual intersection point, and the heater 22 is electrically connected with the transverse heating wires 23 and the longitudinal heating wires 24 at the virtual intersection point, as shown in fig. 5.
When the auxiliary heating module 2 is not connected with the temperature control module 1, as shown in fig. 3 and 4, the sample tube 3 can be directly embedded in the column 11 of the temperature control module 1, and the control module or other heating devices can control the temperature control module 1 to perform main heating on the sample tube 3, however, in this mode, temperature difference exists between a plurality of sample tubes 3, and the temperature uniformity is poor, so as to influence the experimental result. And this application is through setting up auxiliary heating module 2, after auxiliary heating module 2 is connected the completion with accuse temperature module 1, be as shown in fig. 1 the state, at this moment, the sample tube 3 that has the sample of awaiting measuring to run through-hole 21 and stretch into in the holding tank 111 of cylinder 11, thereby accomplish placing of sample tube 3, because the periphery of every through-hole 21 all encloses and establishes heater 22, consequently, the periphery that is equivalent to every cylinder 11 all encloses and establishes heater 22, when heater 22 heats, can heat cylinder 11 through heat transfer, and then with heat transfer to the sample tube 3 in the cylinder 11, play the purpose to the auxiliary heating of sample tube 3, thereby can carry out temperature compensation to sample tube 3, with the relatively poor problem of temperature homogeneity between solving a plurality of sample tubes 3.
Temperature sensor is used for detecting the inside temperature of cylinder 11, can detect the temperature of sample pipe 3 promptly, then temperature sensor can be with signal transmission to control module who detects, temperature through control module control heater 22, when temperature module 1 heats the intensification and handles to the sample that awaits measuring in a plurality of sample pipes 3 promptly, when temperature sensor senses that the inside temperature of part cylinder 11 does not reach preset temperature (for example degeneration temperature), many horizontal heating wires 23 and many vertical heating wires 24 of control module control auxiliary heating module 2 switch on and off in proper order, so that the cylinder 11 that the corresponding heater 22 heating corresponds, thereby accomplish the auxiliary heating to sample pipe 3 through auxiliary heating module 2, make the temperature homogeneity performance between a plurality of sample pipes 3 obtain promoting.
The auxiliary heating principle of the auxiliary heating module 2 is described in detail below.
As shown in fig. 5, a schematic diagram of a connection circuit of the transverse heating wire 23, the longitudinal heating wire 24 and the heater 22 of the auxiliary heating module 2 is shown, wherein one end of the transverse heating wire 23 is used for being electrically connected or disconnected with the control module, and the other end is not connected with the circuit, and similarly, one end of the longitudinal heating wire is used for being electrically connected or disconnected with the control module, and the other end is not connected with the circuit, wherein the control module includes a control program and a power supply inside, the control program controls the positive pole of the power supply to be communicated with the transverse heating wire 23, and the negative pole of the control power supply to be communicated with the longitudinal heating wire 24, so that the transverse heating wire 23, the longitudinal heating wire 24 and the corresponding heater 22 form a loop, so that the heater 22 heats the corresponding column 11, the temperature of the column 11 is raised to a target temperature, the temperature inside the plurality of columns 11 is relatively uniform, and the temperature uniformity of the plurality of sample tubes 3 at the same time is relatively good; after the control module controls the disconnection between at least one of the transverse heating wire 23 and the longitudinal heating wire 24 and the heater 22, the heater 22 is closed, so that the control module can control the opening and closing of the heater 22 by controlling the transverse heating wire 23 and the longitudinal heating wire 24, and the opening and closing of a plurality of heaters 22 can be controlled by a small number of heating wires through the connection mode of the transverse heating wire 23 and the longitudinal heating wire 24 in the longitudinal and transverse arrangement and the control mode of the control module, thereby saving the cost and being more convenient to operate.
The following describes in detail a plurality of auxiliary heating modes of the auxiliary heating module 2.
One heating mode is as follows: the plurality of heaters 22 are individually activated and heated one by one.
Specifically, in some embodiments, one transverse heater wire 23 is energized while the remaining transverse heater wires 23 are not energized, and the longitudinal heater wires 24 are sequentially energized, such that the heaters 22 heat the column 11 one by one (sequentially). For example, the control module controls the first transverse heating wire 23 to be powered on, the rest of the transverse heating wires are not powered on, then the longitudinal heating wires 24 are powered on one by one, and only one longitudinal heating wire 24 is powered on at the same time, that is, when the first longitudinal heating wire 24 is powered on, the rest of the longitudinal heating wires 24 are powered off, when the second longitudinal heating wire 24 is powered on, the first and the other longitudinal heating wires 24 are powered off, when the third longitudinal heating wire 24 is powered on, the first, the second and the other pairs of longitudinal heating wires 24 are powered off, and so on, the longitudinal heating wires 24 are powered on and off in the above manner no matter how many longitudinal heating wires 24 are. After the first row of heaters 22 is heated, the second transverse heating wire 23 is powered on, the first transverse heating wire 23 and other transverse heating wires 23 are powered off, then the longitudinal heating wires 24 are powered on one by one, only one longitudinal heating wire 24 is powered on at the same time, and the like, the power is on and off in the above mode no matter how many transverse heating wires 23 are provided, so that the heaters 22 can be heated one by one. The energization manner here corresponds to that the longitudinal heating lines 24 are energized one by one after each transverse heating line 23 is heated for a certain period of time. Through this kind of matrix control by temperature change auxiliary heating device that this application provided, can be so that a plurality of heaters 22 heat alone to the cylinder 11 that corresponds one by one to when avoiding a plurality of heaters 22 to start simultaneously, take place heat transfer between the cylinder 11, with the auxiliary heating effect that influences cylinder 11, thereby improve in the experimentation, the temperature homogeneity between sample tube 3 improves the accuracy of experimental result.
The control module controls the power-on and power-off sequence of the transverse heating wire 23 and the longitudinal heating wire 24, and the heating power of each heater 22 is designed and recorded and stored in the control program of the control module. For example, the sequence of heating by the heater 22 may be from the top left corner shown in fig. 5, from left to right, and from top to bottom, or from the bottom right corner shown in fig. 5, from right to left, and from bottom to top, or may be other heating sequences, which is not limited herein, as long as the heater 22 is ensured to heat the corresponding columns 11 one by one; in addition, the energization time of each of the transverse heating lines 23 or each of the longitudinal heating lines 24 may be different, so that the energization time of each of the heaters 22 is different to vary the heating power of each of the heaters 22, thereby further improving the temperature uniformity performance between the sample tubes 3. Alternatively, the heaters 22 are arranged in groups, each group including at least two heaters 22, and the heaters 22 are activated in groups, and heat is applied in groups.
Specifically, in some embodiments, one transverse heater wire 23 is energized while the remaining transverse heater wires 23 are not energized and a plurality of longitudinal heater wires 24 are energized to heat the columns 11 of the group of heaters 22. For example, the control module controls the first transverse heating wire 23 to be powered on, the rest of the transverse heating wires are not powered on, then the plurality of longitudinal heating wires 24 are powered on, and the plurality of longitudinal heating wires 24 may be grouped into one group, so that the longitudinal heating wires 24 may be powered on and off in one group, that is, when the first group of longitudinal heating wires 24 is powered on, the rest of the longitudinal heating wires 24 are powered off, when the second group of longitudinal heating wires 24 is powered on, the first group and the other longitudinal heating wires 24 are powered off, and so on, no matter how many groups the longitudinal heating wires 24 have, the above-mentioned manner is adopted. When the first row of heaters 22 is heated, the second transverse heating wire 23 is powered on, the first and other transverse heating wires 23 are powered off, then the longitudinal heating wires 24 are powered on in a group by group, and in the same way, the transverse heating wires 23 are powered on and off no matter how many transverse heating wires 23 are, so that the heaters 22 can be heated in a group. The energization pattern here corresponds to that the longitudinal heating wires 24 are energized in groups after each of the transverse heating wires 23 is heated for a certain period of time.
In other embodiments, while the plurality of transverse heater wires 23 are energized, the remaining transverse heater wires 23 are not energized and the longitudinal heater wires 24 are sequentially energized to heat the columns 11 in groups of heaters 22. For example, the control module controls the plurality of transverse heating wires 23 to be powered on, the rest of the transverse heating wires 23 are not powered on, the plurality of transverse heating wires 23 can be in a group, so that the transverse heating wires 23 can be powered on and off in a group, then the longitudinal heating wires 24 are powered on one by one, only one longitudinal heating wire 24 is powered on at the same time, that is, when the first longitudinal heating wire 24 is powered on, the rest of the longitudinal heating wires 24 are powered off, when the second longitudinal heating wire 24 is powered on, the first and other longitudinal heating wires 24 are powered off, and so on, the longitudinal heating wires 24 are powered on and off in the above manner no matter how many longitudinal heating wires 24 are. After the plurality of rows of heaters 22 are heated, the second group of transverse heating wires 23 is powered on, the first group and other transverse heating wires 23 are powered off, then the longitudinal heating wires 24 are powered on one by one, only one longitudinal heating wire 24 is powered on at the same time, and the power is switched on and off in the above mode no matter how many transverse heating wires 23 are provided, so that the heaters 22 can be heated in groups.
Through this kind of matrix control by temperature change auxiliary heating device that this application provided, can be so that a plurality of heaters 22 heat corresponding cylinder 11 simultaneously to improve auxiliary heating's rate.
In other embodiments, the control module may control the transverse heating wires 23 and the longitudinal heating wires 24 to heat from outside to inside in sequence, at this time, the two longitudinal heating wires 24 at the outside are powered on, the transverse heating wires 23 are powered on one by one, that is, the longitudinal heating wires 24 at the outside are powered on for a period of time and then are disconnected (each time the longitudinal heating wires 24 are powered on for a period of time, the transverse heating wires 23 are powered on one by one), and then the longitudinal heating wires 24 at the inside are also powered on one by one for a period of time and then are disconnected, so that the integral column 11 can be heated. In the heating, the heating power of the outer heater 22 may be larger than the heating power of the inner heater 22.
In some embodiments, the plurality of transverse heater wires 23 are energized for different durations or the plurality of longitudinal heater wires 24 are energized for different durations, so that the heating power is different between the heaters 24. The control module controls the transverse heating wire 23 and the longitudinal heating wire 24 to be powered on at the same time and then not powered off at the same time, so that different heaters 22 can have different heating time to realize non-heating power for different sample tubes 3.
In the temperature control process, the heaters 22 at the intersection are sequentially electrified one by one or electrified one by one to heat the sample tubes 3 one by one or assisted by one, so that the temperature of the sample tubes 3 can reach the same temperature, and the temperature uniformity among the sample tubes 3 and the samples to be measured is improved.
It should be noted that the above steps only show some possible working processes of the temperature control module 1, and that the temperature control module 1 may have other working processes.
The heaters 22 work one by one to be the optimal scheme, and the purpose of the heaters 22 work one by one is to further improve the temperature uniformity of the sample tube 3, prevent the heat conduction generated between the two heaters 22 when the two heaters 22 work simultaneously, thereby influencing the effect of auxiliary heating and further improving the temperature control precision.
It should be noted that the heating power of each heater 22 is not uniform when it is operated, because the temperature of each sample tube 3 is not the same when there is a problem of temperature uniformity between the columns 11, and naturally the heating power of each sample tube 3 is different when it is additionally heated in order to raise the temperature of all the sample tubes 3 to the same temperature. Therefore, when the control module controls each longitudinal heating wire 24 and each transverse heating wire 23 to be electrified, the electrifying power is designed and executed according to the heating power required by the sample tube 3 at the intersection point, and when some sample tubes 3 do not need auxiliary heating, any one or two of the transverse heating wire 23 and the longitudinal heating wire 24 at the sample tube 3 are not electrified, so that the heating power of the heater 22 at the position is zero.
For example, the number of the transverse heating lines 23 is 12, the number of the longitudinal heating lines 24 is 8, and 96 intersections are formed, so that 96 heaters 22 can be designed and distributed in a matrix, and in this case, the number of the sample tubes 3 is also 96 and distributed in a matrix, which corresponds to the number of the heaters 22 around the sample tubes 3. The control module adopts a pulse width modulation technology (PWM technology) to control the transverse heating wire 23 and the longitudinal heating wire 24, and is simultaneously designed to finish one-time auxiliary heating of 96 sample tubes 3 in 1 second or 2 seconds, so that the one-by-one auxiliary heating of the 96 sample tubes 3 can be finished in a very short time, which is similar to the auxiliary heating of the 96 sample tubes 3, and the temperature uniformity performance among a plurality of sample tubes 3 is further improved.
The number of the transverse heating lines 23 may be 8, 12 or other numbers, and the number of the longitudinal heating lines 24 may be 8 or other numbers, which may be determined according to the usage requirement.
The heater 22 may be a heating resistor.
The cylinder 11 is made of a heat conducting material, which may be a heat conducting metal, or other heat conducting materials.
Foretell auxiliary heating module 2 can dismantle with accuse temperature module 1 and be connected, can make things convenient for auxiliary heating module 2's dismantlement. When in use, the auxiliary heating module 2 can be directly placed on the surface of the temperature control module 1, and can also be fixedly connected with the temperature control module 1.
The through holes on the auxiliary heating module 2 are distributed in a matrix form in an m × n manner, wherein m is not less than 2, and n is not less than 2. M here can be set to be 8, 12 or other numbers, n can also be set to be 8 or other numbers, and it can be determined according to the use requirement, for example, two kinds of auxiliary heating modules 2 shown in fig. 2 and 7, when the auxiliary heating module 2 is the case shown in fig. 7, the auxiliary heating module 2 can be installed at any position of the temperature control module 1, so as to perform auxiliary heating on the column 11 here.
In some embodiments, as shown in fig. 3, the adjacent columns 11 are connected by a support platform 12, the support platform 12 is used for supporting the auxiliary heating module 2, the height of the support platform 12 determines the position of the heater 22 relative to the sample tube 3, and the position of the support platform 12 can be changed according to actual use requirements.
The heights of the bearing tables 12 are different, the positions of the heaters 22 are different, and the heaters 22 can be positioned at the upper part, the middle part, the bottom part or other parts of the column 11.
Wherein, the top height of cylinder 11 is higher than the height of plummer 12, consequently forms the recess between cylinder 11 and the plummer 12, and when installation auxiliary heating module 2, auxiliary heating module 2 can block in the recess, consequently can enough play spacing effect. Meanwhile, as the auxiliary heating module 2 is clamped in the groove, the heater 22 can be just attached to the outer wall of the cylinder 11, and therefore heat transfer is easier.
The weight-reducing holes 121 are formed in the bearing table 12, so that the weight of the whole temperature control module 1 can be reduced through the weight-reducing holes 121, and meanwhile, the cost of raw materials can be saved.
In some embodiments, as shown in fig. 6, a mounting groove 13 is formed at the bottom of temperature control module 1, where the mounting groove 13 is used for placing a temperature sensor, and the mounting groove 13 may be multiple, and can be used for installing multiple temperature sensors to detect the temperature inside column 11. The temperature sensor is electrically connected with the control module, and the temperature sensor can transmit the detected signal to the control module to control the temperature of the temperature control module 1 and/or the heater 22 through the control module.
The working process of the matrix temperature control auxiliary heating device for the PCR instrument comprises the following steps:
firstly, the temperature control module 1 and the auxiliary heating module 2 are installed, then the sample tubes 3 penetrate through the through holes 21 on the auxiliary heating module 1 and are finally embedded in the cylinder 11 of the temperature control module 1, then the temperature control module 1 controls the cylinder 11 to be heated to a first target temperature (about 95 ℃), DNA is denatured, at the moment, the temperature sensors can detect the temperature inside the cylinder 11 and transmit detected temperature signals to the control module, when the first temperature sensors sense that the internal temperature of part of the cylinder 11 does not reach the first target temperature, the control module controls the auxiliary heating module 2 to start heating, so that heat generated by the heaters 22 is transmitted to the cylinder 11, the temperature of the cylinder 11 is raised to the first target temperature, the internal temperatures of the cylinders 11 are enabled to be more uniform, the temperature uniformity of the sample tubes 3 at the same moment is enabled to be better, and finally the accuracy of detection results of the sample tubes 3 is enabled to be better; then the temperature control module 1 is used for cooling to a second target temperature (about 60 ℃), so that the sample to be detected in the sample tube 3 is subjected to DNA replication according to the base complementary pairing principle, and the temperature control process is repeated to complete replication and amplification of the sample to be detected.
The heater 22 may be activated singly or in multiple ways, specifically, depending on the signal detected by the temperature sensor, the temperature sensor will transmit the signal to the control module, the control module will control the corresponding transverse heating wire 23 and the longitudinal heating wire 24 to be powered on, so that the heater 22 at the intersection of the transverse heating wire 23 and the longitudinal heating wire 24 is communicated with the transverse heating wire 23 and the longitudinal heating wire 24, the heater 22 will activate heating, and then transmit heat to the column 11 around the heater 22, so as to raise the temperature of the column 11 to the first target temperature.
Referring to fig. 8, an embodiment of the present disclosure provides a PCR instrument, including: heat abstractor 4 and the matrix control by temperature change auxiliary heating device who is used for the PCR appearance, a matrix control by temperature change auxiliary heating device for the PCR appearance is located heat abstractor 4 tops, and a heater 22 for the matrix control by temperature change auxiliary heating device of PCR appearance has different heating power, has solved the poor problem of temperature homogeneity.
The structure of the matrix temperature control auxiliary heating device for a PCR instrument in this embodiment includes the same or similar technical effects as described above, and therefore, detailed description is not repeated herein, and reference may be made to the description of the matrix temperature control auxiliary heating device for a PCR instrument.
In the description of the present invention, it is to be understood that the indicated orientations or positional relationships are based on the orientations or positional relationships shown in the drawings and are only for convenience in describing the present invention and simplifying the description, but are not intended to indicate or imply that the indicated devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be construed as limiting the present invention.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. The utility model provides a matrix control by temperature change auxiliary heating device for PCR appearance which characterized in that includes:
the temperature control module comprises a plurality of columns, and an accommodating groove for accommodating the sample tube is formed in each column so that the sample tube can be adjusted in the accommodating groove;
the auxiliary heating module is connected with the temperature control module, a plurality of through holes distributed in a matrix form are formed in the auxiliary heating module, the cylinder penetrates through the through holes, a plurality of transverse heating wires and a plurality of longitudinal heating wires are further arranged on the auxiliary heating module, the transverse heating wires are located on one row of the through holes, one transverse heating wire corresponds to one row of the through holes, the longitudinal heating wires are located on one row of the through holes, one longitudinal heating wire corresponds to one row of the through holes, the transverse heating wires and the longitudinal heating wires are arranged in a staggered mode to form intersection points, heaters are arranged at least on part of the intersection points, and the transverse heating wires and the longitudinal heating wires at the corresponding intersection points are electrically connected with the heaters;
the control module controls the plurality of transverse heating wires and the plurality of longitudinal heating wires to be sequentially switched on or off, and when the control module is switched on, the transverse heating wires, the longitudinal heating wires and the corresponding heaters form a loop so that the corresponding heaters heat the corresponding cylinders;
the temperature sensor is used for detecting the temperature inside the cylinder, transmitting a detected signal to the control module, and controlling the temperature of the heater through the control module.
2. The matrix temperature control auxiliary heating device for PCR instrument as claimed in claim 1, wherein when one of said transverse heating wires is energized, the other transverse heating wires are not energized, and a plurality of said longitudinal heating wires are sequentially energized, and only one of said longitudinal heating wires is energized at a time, so that said heaters heat the corresponding said cartridges one by one.
3. The matrix temperature control auxiliary heating device for the PCR instrument as claimed in claim 1, wherein when one of said transverse heating lines is energized, the other transverse heating lines are not energized, and at least two of said longitudinal heating lines are energized simultaneously in groups, so that said heaters heat the corresponding said columns in groups.
4. The matrix temperature-controlled auxiliary heating device for PCR instrument according to claim 2 or 3, wherein the duration of the power-on of the plurality of transverse heating lines is different or the duration of the power-on of the plurality of longitudinal heating lines is different, so that the heating power is different between the heaters.
5. The matrix temperature-controlled auxiliary heating device for the PCR instrument as claimed in any one of claims 1 to 3, wherein the auxiliary heating module is detachably connected to the temperature control module.
6. The matrix temperature control auxiliary heating device for the PCR instrument as claimed in any one of claims 1 to 3, wherein adjacent ones of the cartridges are connected by a support platform for supporting the auxiliary heating module.
7. The matrix temperature-controlled auxiliary heating device for PCR instrument of claim 6, wherein the top of the cartridge is higher than the height of the platform.
8. The matrix temperature-control auxiliary heating device for the PCR instrument as claimed in claim 6, wherein the bearing platform is provided with lightening holes.
9. The matrix temperature control auxiliary heating device for PCR instrument of claim 1, wherein said through holes on said auxiliary heating module are distributed in matrix form in m x n manner, wherein said m is not less than 2, and said n is not less than 2.
10. A PCR instrument, comprising: a heat sink and the matrix temperature control auxiliary heating device for PCR instrument as claimed in any one of claims 1-9, wherein the matrix temperature control auxiliary heating device for PCR instrument is located above the heat sink.
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| CN202211425071.7A CN115572675A (en) | 2022-11-15 | 2022-11-15 | Matrix temperature control auxiliary heating device for PCR instrument and PCR instrument |
| CN202322092173.8U CN220550183U (en) | 2022-11-15 | 2023-08-04 | Matrix type temperature control auxiliary heating device for PCR instrument and PCR instrument |
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| CN202211425071.7A CN115572675A (en) | 2022-11-15 | 2022-11-15 | Matrix temperature control auxiliary heating device for PCR instrument and PCR instrument |
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| CN202322092173.8U Active CN220550183U (en) | 2022-11-15 | 2023-08-04 | Matrix type temperature control auxiliary heating device for PCR instrument and PCR instrument |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0552666A (en) * | 1991-08-27 | 1993-03-02 | Tokai Carbon Co Ltd | Plane temperature measurement sensor |
| JPH11287831A (en) * | 1998-04-01 | 1999-10-19 | Canon Inc | Method and device for measuring resistance, and device of electronic source and its manufacture |
| JP2001311757A (en) * | 2000-04-28 | 2001-11-09 | Nec Corp | Inspecting device for array type electronic component |
| CN200988331Y (en) * | 2006-11-23 | 2007-12-12 | 黄生新 | Matrix type Electric heating table for printing pattern |
| CN101974421A (en) * | 2010-10-26 | 2011-02-16 | 秦荣 | Variable-temperature metal module with precise temperature compensation and temperature compensating method |
| JP2011240726A (en) * | 2010-05-14 | 2011-12-01 | Toyota Boshoku Corp | Temperature distribution detection device, human body detector, and seat for vehicle |
| CN210481399U (en) * | 2019-05-30 | 2020-05-08 | 杭州安誉科技有限公司 | Auxiliary heating device for gene amplification instrument module |
-
2022
- 2022-11-15 CN CN202211425071.7A patent/CN115572675A/en active Pending
-
2023
- 2023-08-04 CN CN202322092173.8U patent/CN220550183U/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0552666A (en) * | 1991-08-27 | 1993-03-02 | Tokai Carbon Co Ltd | Plane temperature measurement sensor |
| JPH11287831A (en) * | 1998-04-01 | 1999-10-19 | Canon Inc | Method and device for measuring resistance, and device of electronic source and its manufacture |
| JP2001311757A (en) * | 2000-04-28 | 2001-11-09 | Nec Corp | Inspecting device for array type electronic component |
| CN200988331Y (en) * | 2006-11-23 | 2007-12-12 | 黄生新 | Matrix type Electric heating table for printing pattern |
| JP2011240726A (en) * | 2010-05-14 | 2011-12-01 | Toyota Boshoku Corp | Temperature distribution detection device, human body detector, and seat for vehicle |
| CN101974421A (en) * | 2010-10-26 | 2011-02-16 | 秦荣 | Variable-temperature metal module with precise temperature compensation and temperature compensating method |
| CN210481399U (en) * | 2019-05-30 | 2020-05-08 | 杭州安誉科技有限公司 | Auxiliary heating device for gene amplification instrument module |
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| CN220550183U (en) | 2024-03-01 |
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