CN115429917B

CN115429917B - Sterilizing device for plant tissue culture explant

Info

Publication number: CN115429917B
Application number: CN202211080110.4A
Authority: CN
Inventors: 宋慧
Original assignee: Ningbo Academy of Agricultural Sciences
Current assignee: Ningbo Academy of Agricultural Sciences
Priority date: 2022-09-05
Filing date: 2022-09-05
Publication date: 2024-01-30
Anticipated expiration: 2042-09-05
Also published as: CN115429917A

Abstract

The invention relates to the field of tissue culture, in particular to a sterilization device for plant tissue culture explants, which comprises: the device comprises a device body, wherein a sterilization area and a liquid storage area are separated from top to bottom through a partition board, the partition board is provided with a liquid leakage hole, and the liquid storage area is provided with a liquid drainage hole; the panel is positioned at the top of the device body; the filter screen is arranged in the sterilization area through the panel; the rocking handle drives the stirring assembly to rotate in the filter screen; further comprises: the valve assembly is provided with a valve gasket, and the valve gasket seals the liquid leakage hole; the fixed plate is connected with the partition plate, the fixed plate is provided with a mounting hole for mounting the valve component, and the upper part of the valve component is sleeved with a spring and can stretch and retract relative to the mounting hole; one end of the valve driving mechanism is connected with the top of the valve component; the other end of the button is contacted with a button of a handle of the device body. Through button, valve actuating mechanism, valve subassembly and spring, make the sealed weeping hole of valve pad, guarantee that the valve pad is closed and keep producing precompression to baffle weeping hole to improve the sealed effect of shutoff.

Description

Sterilizing device for plant tissue culture explant

Technical Field

The invention relates to the technical field of tissue culture, belongs to the technical field of plant biology in the agriculture and forestry field, and particularly relates to a sterilization device for a plant tissue culture explant.

Background

The sterilization of the explant is the first step of tissue culture, and involves the links of alcohol washing, disinfectant washing, sterile water washing and the like. Generally, tissue culture personnel place the explants in sterile culture flasks, repeatedly pour the liquid in the order of alcohol-sterile water-disinfectant-sterile water, burn the glass rod or forceps with an alcohol lamp during the process to make the glass rod or forceps sterile, and then stir the liquid to increase the contact between the disinfectant and the explants. These repeated pouring operations are not only cumbersome, but also increase the risk of the explant being poured out together during the pouring of the liquid. The glowing glass rods or tweezers enter alcohol or disinfectant, so that on one hand, alcohol combustion can be caused to cause fire hazard, and on the other hand, the disinfectant can be heated to decompose to release harmful gases. Therefore, the existing sterilization device and technology are not simple and convenient to operate, the risk of fire and harmful gas release is high, the explant is easy to lose along with pouring liquid, and a simple and labor-saving explant sterilization device is needed, so that the tissue culture efficiency is improved.

Patent document CN 215460434U discloses a simple sterilization device for culturing plant tissue explants, comprising a container, a partition plate, a control rod, a blocking ball and a filter screen basket, wherein the top end of the container is provided with an opening; the edge of the baffle plate is fixed with the inner wall of the container and divides the interior of the container into an upper disinfection cavity and a lower liquid outlet cavity, a liquid leakage hole is arranged at a position, close to one side, of the baffle plate, and a liquid discharge hole is arranged at the bottom wall of the container corresponding to the liquid outlet cavity; one end of the operating rod is vertically inserted into the container; the blocking ball is fixed at one end of the control rod, which is positioned in the container, and can block the liquid leakage hole; the problem of above-mentioned explant sterilization operation inconvenient has been solved to it, but because the stopper ball of adoption control lever drive blocks up the weeping hole, the stopper ball of plastics is by self weight and connecting rod dead weight to block up baffle weeping hole, but this structure is owing to own connecting rod dead weight is very light, and this structure leakproofness can not guarantee, if put on having the uneven face in slope also can appear leaking the condition, and the stopper ball water shutoff is not tight, can lead to the antiseptic solution to remain effectively in the disinfection chamber, can not accomplish the explant sterilization operation, and then can't satisfy sterilization apparatus normal use.

Therefore, how to provide a sterilization device for plant tissue culture explants solves the problem that the water shutoff sealing is unreliable in the prior art, and is a problem which needs to be solved by the technicians in the field.

Disclosure of Invention

Therefore, one purpose of the invention is to provide a sterilization device for plant tissue culture explants, which solves the problem that the sealing and water plugging of the weeping holes are unreliable in the prior art.

The invention provides a sterilization device for plant tissue culture explants, which comprises:

the device comprises a device body, wherein the device body is internally divided into a sterilization area and a liquid storage area from top to bottom through a partition board, the partition board is provided with a liquid leakage hole, and the liquid storage area is provided with a liquid discharge hole;

a panel positioned on top of the device body;

the filter screen is arranged in the sterilization zone through the panel;

the rocking handle drives the stirring assembly to rotate in the filter screen;

further comprises:

a valve assembly having a valve gasket sealing the weep hole;

the fixed plate is connected with the partition plate, a mounting hole for mounting the valve component is formed in the fixed plate, and a spring is sleeved on the upper part of the valve component and can stretch relative to the mounting hole; and

one end of the valve driving mechanism is connected with the top of the valve component; the other end of the button is contacted with a button of a handle of the device body.

Further, the valve assembly further comprises:

the top of the connecting seat is used for installing a spring and is matched with the installing hole, and the middle of the connecting seat is a channel;

the valve connecting rod, the valve pad is cyclic annular, and its cover is located valve connecting rod bottom is in order to seal the weeping hole, the top of valve connecting rod stretches into the passageway, with the connecting seat is fixed the valve pad.

Further, the valve pad is a silica gel pad.

Further, the valve driving mechanism includes:

the connecting rod fixing cap is characterized in that the top surface of the fixing plate, which is opposite to the mounting hole, is a mounting plane which is sunken downwards, the top of the connecting seat extends out of the mounting plane and is clamped with the connecting rod fixing cap, and the top of the connecting rod fixing cap is provided with an insertion groove;

the connecting pin enters the connecting rod fixing cap through the insertion groove;

the transmission rod, transmission rod one end with the connecting pin is connected, and the connecting axle is installed to its middle part, the connecting axle support in shaft hole or draw-in groove on the handle, the transmission rod other end with the inside butt of button.

Further, the connecting pin is I-shaped with a long upper part and a short lower part, the bottom of the connecting pin is not separated from the inserting groove, and the top of the connecting pin is connected with one end of the transmission rod.

Further, the connecting end of the transmission rod and the connecting pin adopts a hook sleeve structure with an upward opening, and a pin entering groove is formed in one end, close to the hook sleeve structure, of the transmission rod; the transmission rod takes the connecting shaft as a demarcation point, the connecting end of the transmission rod is horizontal to the whole connecting shaft, the contact section of the connecting shaft to the button is in a shape of lowering first and then lifting second, and the pressing end of the button is in contact with the position of the lifting shape of the contact section.

Further, the rocking handle is detachably connected with the stirring assembly.

Further, the stirring assembly includes:

a cover adapted to fit the screen top opening; a bearing is arranged in the bearing;

the stirring rod is inserted from the top of the cover, matched with the bearing and fixed from the bottom of the cover through a nut; the top of the stirring rod is provided with a driving hole, and the driving hole is connected with the rocking handle or the driving end of the motor;

and the blade is fixed at the bottom end part of the stirring rod through a screw.

Further, the partition plate is a cylinder body with an open top, the drain hole is positioned at the lowest part of the bottom wall of the drain hole, and the side wall of the drain hole is welded with the device body in an ultrasonic mode.

Further, the liquid discharge hole is located at the lower portion of the device body and located on the outer wall of the side opposite to the handle, a liquid discharge tube head is installed at the liquid discharge hole, the liquid discharge tube head is connected with a liquid discharge cover, and a sealing gasket is arranged between the liquid discharge cover and the liquid discharge tube head.

Compared with the prior art, the invention discloses a sterilization device for plant tissue culture explants, which has the following beneficial effects:

1. the valve driving mechanism is pressed by the button, the valve driving mechanism directly presses down the valve assembly to enable the valve gasket to seal the liquid leakage hole, and a compressible spring is additionally arranged on the valve assembly, so that the valve gasket is ensured to always keep generating precompression on the liquid leakage hole of the partition plate under the action of the compression spring force when the valve gasket is closed and does not work, and therefore a good water shutoff sealing effect is achieved, and the valve gasket can play a good water shutoff effect even being placed on an inclined plane with a gradient;

2. the plane of the baffle plate is modified and adjusted, the original plane is changed into a funnel-like shape, the liquid leakage hole of the baffle plate is made to be the lowest, the edge of the baffle plate is the highest, and the edge of the baffle plate is 1mm higher than the liquid leakage hole, so that when the silica gel valve pad is opened, all liquid on the baffle plate can be converged to the liquid leakage hole of the baffle plate to form liquid accumulation state;

3. the stirring assembly is additionally provided with a bearing, so that the rocking handle can be easily rotated, and abnormal sound generated by friction resistance and friction between plastics is solved, thereby driving the blades to rotate;

4. the silica gel valve pad is opened in a connecting rod transmission mode, a plastic button is arranged on the plastic handle, when the button is not pressed down, one end of the transmission rod is downward under the acting force of the button, so that the other end is upward tilted to drive the valve connecting rod and the silica gel valve pad to be upward lifted, a water leakage hole of the partition plate is in an opened state, liquid flows into a liquid storage area through the hole, the connecting rod transmission opening mode of the button enables the device to have better water shutoff sealing effect, no water leakage is caused, the structure is more reliable, the application environment is wider, and a good water shutoff effect can be achieved even if the device shakes or is placed on an inclined plane in the operation process;

5. the structural component is convenient for mold opening production and product later assembly and assembly, and can be produced by adopting a 3D printing technology.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.

FIG. 1 is an exploded view of a sterilization apparatus for plant tissue culture explants according to the present invention;

FIG. 2 is a longitudinal cross-sectional view of a sterilization apparatus for plant tissue culture explants according to the present invention;

FIG. 3 is an exploded view of the stirring assembly;

FIG. 4 is an exploded view of the valve assembly;

FIG. 5 is an exploded view of the drive rod, connecting pin and connecting shaft;

FIG. 6 is an exploded view of the drive rod, connecting shaft, connecting rod fixing cap and fixing plate;

FIG. 7 is a schematic cross-sectional view of an assembled valve drive mechanism and valve assembly;

FIG. 8 is an exploded view of the valve actuator and valve assembly;

FIG. 9 is a schematic diagram showing the structure of a sterilization apparatus for plant tissue culture explants.

Detailed Description

Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present invention and should not be construed as limiting the invention.

In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.

Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.

In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.

In view of the problem that the sealing and water blocking of the liquid leakage holes are unreliable in the prior art, the blocking ball is not tight in water blocking, so that the disinfectant can not be effectively reserved in the disinfection cavity, the sterilization operation of the explant can not be completed, and the sterilization device can not be normally used. The embodiment of the invention discloses a sterilization device for a plant tissue culture explant, which comprises the following components: the device comprises a device body 11, wherein a sterilization area and a liquid storage area are separated from top to bottom in the device body 11 through a partition board 10, the partition board 10 is provided with a liquid leakage hole, and the liquid storage area is provided with a liquid discharge hole; a panel 4, the panel 4 being located on top of the device body 11; a filter screen 3, wherein the filter screen 3 is installed in the sterilization zone through the panel 4; the stirring device comprises a rocking handle 1, wherein the rocking handle 1 drives a stirring assembly 2 to rotate in a filter screen 3;

referring to fig. 1, 2 and 9, the method further includes: a valve assembly 9, the valve assembly 9 having a valve pad 91, the valve pad 91 sealing the weep hole; the fixed plate 8 is connected with the partition plate 10, a mounting hole for mounting the valve component 9 is formed in the fixed plate 8, and a spring 92 is sleeved on the upper part of the valve component 9 and can stretch and retract relative to the mounting hole; and a valve driving mechanism 6, wherein one end of the valve driving mechanism 6 is connected with the top of the valve assembly 9; the other end of which is in contact with the push button 5 of the handle 14 of the device body 11.

By the arrangement, the invention still keeps the sterilization of the explant in the filter screen at the upper layer of the sterilization device, and the waste liquid is discharged to the liquid storage area at the lower layer of the sterilization device through the valve, so that repeated pouring of the liquid such as disinfectant, sterile water and the like is not needed; the blade is rotated by the rocking handle to stir the disinfectant and the sterile water, so that the contact between the disinfectant and the explant can be increased without burning a glass rod or tweezers and stirring the disinfectant.

Meanwhile, the valve driving mechanism can be pressed by a button, the valve driving mechanism directly presses down the valve assembly, so that the valve gasket seals the liquid leakage hole, a compressible spring is added on the valve assembly, so that the valve gasket is ensured to be always kept to generate precompression on the baffle liquid leakage hole under the action of the compression spring force when the valve gasket is closed and does not work, thereby playing a good water shutoff sealing role, playing a good water shutoff role even if the valve gasket is placed on an inclined plane with a gradient, and ensuring the normal use of the sterilization device.

Referring to fig. 1 and 4, the valve assembly 9 further includes: the top of the connecting seat 93 is used for installing a spring 92 and is matched with the installing hole, and the middle of the connecting seat 93 is a channel; the valve connecting rod 94, the valve pad 91 is annular, and is sleeved at the bottom of the valve connecting rod 94 to seal the liquid leakage hole, and the top of the valve connecting rod 94 extends into the channel to fix the valve pad 91 with the connecting seat 93.

Preferably, the valve pad 91 is a silica gel pad.

Referring to fig. 5 and 6, the valve driving mechanism 6 includes: the top surface of the connecting rod fixing cap 63, opposite to the mounting hole, of the fixing plate 8 is a mounting plane 81 recessed downwards, the top of the connecting seat 93 extends out of the mounting plane 81 and is clamped with the connecting rod fixing cap 63, and the top of the connecting rod fixing cap 63 is provided with an insertion groove; a connection pin 62, wherein the connection pin 62 enters the connecting rod fixing cap 63 through the insertion groove; and one end of the transmission rod 61 is connected with the connecting pin 62, the middle part of the transmission rod 61 is provided with a connecting shaft 64, the connecting shaft 64 is supported in a shaft hole or a clamping groove on the handle 14, and the other end of the transmission rod 61 is in abutting connection with the inside of the button 5.

Advantageously, the connecting pin 62 is in the shape of an i-shaped with a long upper part and a short lower part, the bottom of which is not separated from the insertion groove, and the top of which is connected to one end of the driving rod 61.

More advantageously, the connecting end of the transmission rod 61 and the connecting pin 62 adopts a hook sleeve structure with an upward opening, the hook sleeve is at least a 3/4 circular arc sleeve, and a pin entering groove is arranged at one end of the transmission rod 61 close to the hook sleeve structure; the transmission rod 61 takes the connecting shaft 64 as a demarcation point, the whole connecting end of the transmission rod is horizontal to the connecting shaft 64, the contact section from the connecting shaft 64 to the button 5 is in a shape of lowering first and then lifting second, and the pressing end of the button 5 is in contact with the position of the lifting shape of the contact section.

In the embodiment of the invention, the rocking handle 1 is detachably connected with the stirring assembly 2.

In a specific embodiment of the present invention, referring to fig. 3, the stirring assembly 2 includes:

a cover 21, wherein the cover 21 is matched with the top opening of the filter screen 3; within which is disposed a bearing 22;

a stirring rod 23, wherein the stirring rod 23 is inserted from the top of the cover 21, matched with the bearing 22, and fixed from the bottom of the cover 21 through a nut 24; the top of the stirring rod 23 is provided with a driving hole, and the driving hole is connected with the rocking handle 1 or the driving end of the motor;

and a blade 25, wherein the blade 25 is fixed to the bottom end of the stirring rod 23 by a screw 92.

In one embodiment of the present invention, the partition board 10 is a hollow cylinder with an open top, the liquid leakage hole is located at the lowest position of the bottom wall, and the side wall of the liquid leakage hole is welded with the device body 11 by ultrasonic waves.

Referring to fig. 1 and 9, the drain hole is located at the lower part of the device body 11 and on the outer wall opposite to the handle 14, a drain pipe head is installed at the drain hole, the drain pipe head is connected with a drain cover 12, and a sealing pad 13 is arranged between the drain cover 12 and the drain pipe head.

The structure assembly process of the invention is as follows:

referring to fig. 1, 7 and 8, the diaphragm 10 is fixed to the apparatus body 11 by ultrasonic welding, then the valve connecting rod 94 is passed through the middle circular hole of the valve pad 91, the valve pad 91 is simultaneously fixed by the connecting seat 93, the assembly of the valve assembly 9 is completed, then the spring 92 is mounted on the valve assembly 9, then the spring is mounted together in the mounting hole on the upper surface of the fixing plate 8, then the head of the valve assembly 9 is mounted by the connecting rod fixing cap 63 and then rotated by 90 degrees to clamp, so that the valve assembly 9 is fixed on the fixing plate 8, then the fixing plate 8 is fixed on the diaphragm 10 by four screws 7, meanwhile, the valve pad in the valve assembly just blocks the liquid leakage hole of the diaphragm 10, then the connecting rod pin 62 is mounted in the connecting rod fixing cap 63, then the connecting rod pin 62 is hooked by one end of the transmission rod 6, simultaneously the connecting shaft 64 is inserted into the positioning hole of the transmission rod 6, simultaneously the two heads of the connecting shaft 64 are mounted on the groove of the apparatus body 11, or clamped on the shaft hole, then the button 5 is mounted in the groove of the apparatus body 11, the handle 14 is mounted on the apparatus body 11, finally the panel 11 is buckled on the apparatus body 11, and finally the liquid is buckled on the apparatus body 12 by the sealing plate 12.

The stirring assembly 2 is assembled, the bearing 22 is placed in the cover 21, then the bearing 22 is fixed on the cover 21 through three screws 28 by utilizing the bearing pressing plate 27, meanwhile, the bearing 22 is pressed, the stirring rod 23 is inserted from the upper surface of the cover 21, then the stirring rod 23 is screwed and fixed through screw threads below the cover 21 and above the stirring rod 23 through the screw cap 24, finally, the blade 25 is fixed on the stirring rod 23, and then the stirring assembly 2 is assembled through the connection of the screws 26.

Finally, the stainless steel filter screen 3 is placed on the panel 4, then the stirring assembly is covered, and the rocking handle 1 is inserted into the corresponding hole of the stirring rod 23 in the stirring assembly 2, so that all the assembly is completed, and the functions are realized.

In the following, the application of the present invention will be described by taking only 100 pumpkin shelled kernels as an example.

1. The sterilization apparatus was first placed in a sterilization bag and autoclaved at 121 c/0.2 mpa for 15 minutes.

2. After cooling, the sterilization bag is opened on an ultra-clean bench which has been subjected to ultraviolet sterilization for 25 minutes, and the sterilization device is taken out.

3. The stirring assembly 2 is taken down, and pumpkin kernels are put into a stainless steel filter screen 3. The valve assembly 9 of the sterilization device is now in a closed state under the influence of the spring force.

4. Pouring 75% alcohol, covering the stirring assembly 2, inserting the rocking handle 1 into the corresponding driving hole of the stirring rod 23, rocking the rocking handle 1 to drive the blade 25 to rotate, and sterilizing for 30 seconds. The button 5 is pressed, the connecting rod pin 62 is driven by the lever principle of the transmission rod 6, the connecting rod fixing cap 63 is lifted upwards, the valve assembly 9 is driven to overcome the compression force of the spring and lift upwards, the valve pad 91 in the valve assembly 9 leaves the liquid leakage hole of the partition board 10, the liquid leakage hole is in an open state, alcohol is discharged to a liquid storage area through the liquid leakage hole of the partition board 10, the button 5 is loosened, and the valve assembly 9 resets under the action of the compression spring to enable the valve pad 91 to be attached to the liquid leakage hole of the partition board 10, so that the liquid leakage hole is closed.

5. Pouring sterile water, covering the stirring assembly, inserting the rocking handle 1 into the corresponding driving hole of the stirring rod 23, rocking the rocking handle 1 to drive the blades 25 to rotate, and cleaning for 30 seconds. Pressing the button 5, the valve assembly is opened, and the sterile water is discharged to the liquid storage area through the water leakage hole of the partition board 10. The button 5 is released, and the valve assembly is reset to be closed under the action of the compression spring.

6. Step 5 is repeated twice.

7. Pouring 10% sodium hypochlorite solution, covering the stirring assembly, inserting the rocking handle 1 into the corresponding driving hole of the stirring rod, rocking the rocking handle 1 to drive the blade 25 to rotate, and sterilizing for 8 minutes. Pressing the button 5, opening the valve assembly, and discharging sodium hypochlorite to the liquid storage area through the water leakage holes of the partition board 10. The button 5 is released and the valve assembly is reset closed.

8. Pouring sterile water, covering the stirring assembly, inserting the rocking handle 1 into the corresponding driving hole of the stirring rod, rocking the rocking handle to drive the blade to rotate, and cleaning for 30 seconds. The plastic button 5 is pressed, the valve assembly is opened, and the sterile water is discharged to the liquid storage area through the water leakage holes of the partition board 10. The plastic button 5 is released and the valve assembly is reset closed.

9. Step 8 is repeated twice.

10. And (5) finishing the sterilization of the explant. Transferring pumpkin kernels in the filter screen for standby.

11. Unscrewing the liquid discharge cap 12 below the sterilization device and pouring the waste liquid. Cleaning all parts of the sterilizing device, and reusing after high-pressure sterilization.

11. Pumpkin kernels in the filter screen were inoculated in 0.6% agar medium at a density of 10 kernels per petri dish. Culturing for 7 days under the photoperiod condition that the temperature is (25+/-1) DEG C, the illumination intensity is 30000lux and the illumination/darkness is 16h, and counting the pollution rate and the pollution rate is zero.

The invention solves the problems of complex sterilization operation of the explant, easy loss of the explant and more accessories of a sterilization device in the traditional tissue culture process. The device is simple, the operation is simple and easy and labor-saving, the device is suitable for sterilization of various explants, and the popularization value is high. By changing the material and the water blocking structure of the existing blocking ball, a better water blocking effect is realized; the opening direction of the side cover is modified, so that the operation comfort level of pouring waste liquid is improved; the inclination angle of the partition board is increased, so that the water leakage hole of the partition board is at the lowest position, thereby facilitating the better discharge of the sterilizing solution and avoiding the residue in the partition board; the stirring assembly is additionally provided with a bearing, so that the rocking handle can rotate easily without friction noise, in addition, the rocking handle is convenient to insert and pull out, and when the rocking handle is pulled out of the outlet, a driving hole of the stirring rod can be connected with a motor port, so that electric high-speed rotation stirring can be realized; the driving mechanism for sealing the liquid leakage hole of the partition plate adopts a transmission mode of a connecting rod and a compression spring, so that the water shutoff and leakage prevention structure is more reliable.

In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Further, one skilled in the art can engage and combine the different embodiments or examples described in this specification.

While embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the invention, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the invention.

Claims

1. A sterilization apparatus for plant tissue culture explants, comprising:

the device comprises a device body (11), wherein a sterilization area and a liquid storage area are separated from each other in the device body (11) from top to bottom through a partition board (10), the partition board (10) is provided with a liquid leakage hole, and the liquid storage area is provided with a liquid leakage hole;

a panel (4), the panel (4) being located on top of the device body (11);

the filter screen (3) is arranged in the sterilization area through the panel (4);

the stirring device comprises a rocking handle (1), wherein the rocking handle (1) drives a stirring assembly (2) to rotate in a filter screen (3);

characterized by further comprising:

-a valve assembly (9), the valve assembly (9) having a valve gasket (91), the valve gasket (91) sealing the weep hole;

the fixing plate (8), the said fixing plate (8) connects with said baffle (10), there are mounting holes to mount the said valve assembly (9) on it, the upper portion of the said valve assembly (9) is fitted with the spring (92), and it is telescopic to the said mounting hole; and

a valve driving mechanism (6), wherein one end of the valve driving mechanism (6) is connected with the top of the valve assembly (9); the other end of the device body (11) is contacted with a button (5) of a handle (14);

the valve assembly (9) further comprises: the top of the connecting seat (93) is used for installing a spring (92) and is matched with the installing hole, and the middle of the connecting seat is a channel; the valve connecting rod (94), the valve pad (91) is annular, the valve pad is sleeved at the bottom of the valve connecting rod (94) to seal the liquid leakage hole, the top of the valve connecting rod (94) stretches into the channel, and the valve pad (91) is fixed with the connecting seat (93);

the valve driving mechanism (6) includes:

the connecting rod fixing cap (63), the top surface of the fixing plate (8) opposite to the mounting hole is a mounting plane (81) recessed downwards, the top of the connecting seat (93) extends out of the mounting plane (81) and is clamped with the connecting rod fixing cap (63), and the top of the connecting rod fixing cap (63) is provided with an insertion groove;

a connection pin (62), wherein the connection pin (62) enters the connecting rod fixing cap (63) through the insertion groove;

the transmission rod (61), one end of the transmission rod (61) is connected with the connecting pin (62), a connecting shaft (64) is arranged in the middle of the transmission rod, the connecting shaft (64) is supported in a shaft hole or a clamping groove on the handle (14), and the other end of the transmission rod (61) is in butt joint with the inside of the button (5);

the connecting pin (62) is I-shaped with a long upper part and a short lower part, the bottom of the connecting pin is not separated from the insertion groove, and the top of the connecting pin is connected with one end of the transmission rod (61);

the connecting end of the transmission rod (61) and the connecting pin (62) adopts a hook sleeve structure with an upward opening, and a pin entering groove is formed in one end, close to the hook sleeve structure, of the transmission rod; the transmission rod (61) takes the connecting shaft (64) as a demarcation point, the connecting end of the transmission rod is horizontally arranged on the whole connecting shaft (64), the contact section from the connecting shaft (64) to the button (5) is in a shape of lowering before lifting, and the pressing end of the button (5) is in contact with the position of the lifting shape of the contact section.

2. The sterilization apparatus of a plant tissue culture explant according to claim 1, wherein said valve pad (91) is a silicone pad.

3. A plant tissue culture explant sterilization apparatus according to claim 1 or 2, characterized in that said rocking handle (1) is detachably connected to said agitation assembly (2).

4. A plant tissue culture explant sterilization apparatus according to claim 3, characterized in that said agitation assembly (2) comprises:

a cover (21), wherein the cover (21) is matched with the top opening of the filter screen (3); a bearing (22) is arranged in the bearing;

a stirring rod (23), wherein the stirring rod (23) is inserted from the top of the cover (21), matched with the bearing (22) and fixed from the bottom of the cover (21) through a nut (24); the top of the stirring rod (23) is provided with a driving hole, and the driving hole is connected with the rocking handle (1) or the driving end of the motor;

and the blade (25) is fixed on the bottom end part of the stirring rod (23) through a screw (26).

5. A plant tissue culture explant sterilization apparatus according to claim 1 or 2, characterized in that said partition (10) is a hollow cylinder with an open top, said weep hole is located at the lowest part of its bottom wall, and its side wall is ultrasonically welded to said apparatus body (11).

6. Sterilization device for plant tissue culture explants according to claim 1 or 2, characterized in that the drain hole is located in the lower part of the device body (11) and on the outer wall on the side opposite to the handle (14), a drain pipe head is mounted at the drain hole, the drain pipe head is connected with a drain cover (12), and a sealing gasket (13) is provided between the drain cover (12) and the drain pipe head.