CN115428859A

CN115428859A - NMN/vitamin B group and trace element efficacy composition with good pet palatability and preparation method thereof

Info

Publication number: CN115428859A
Application number: CN202211116140.6A
Authority: CN
Inventors: 陈建生; 刘蕊莉; 张彤
Original assignee: Hoboomlife Bio Technology Shenzhen Co Ltd
Current assignee: Hoboomlife Bio Technology Shenzhen Co Ltd
Priority date: 2022-09-14
Filing date: 2022-09-14
Publication date: 2022-12-06

Abstract

The invention discloses an NMN/vitamin B group and trace element efficacy composition with good pet palatability, which comprises the following components: the functional components comprise: NMN, vitamin B group and trace elements; a major component comprising: high fructose corn syrup, amino acid raw material and yeast extract 708. Through the improvement of the palatability of NMN, vitamin B group and trace element liquid, the high fructose corn syrup, the amino acid raw material and the yeast extract 708 raw material are utilized to prepare the pet fur nutrition supplementing micromolecule liquid preparation which has a certain meat flavor, rich meat quality, can well cover the bad smell of the vitamin B group and the trace element, has the stability at normal temperature of 18 months and is easy to absorb.

Description

NMN/vitamin B group and trace element efficacy composition with good pet palatability and preparation method thereof

Technical Field

The invention relates to the technical field of pet foods, in particular to an NMN/vitamin B group and trace element functional composition with good pet palatability and a preparation method thereof.

Background

The cat belongs to absolute carnivorous animals, has extremely high requirements on palatability of cat food, is a technical problem in the world in development of the flavoring agent for the cat, and can be used as a companion animal, the cat has lasting health and prolonged service life, not only can reduce diseases suffered by people, but also can accompany the owner for a long time, and is easy to be favored on the body of the owner and the psychological health.

NAD + levels steadily decline with age, which leads to metabolic changes and increased disease susceptibility. Fluctuations in NAD + can affect: mitochondrial function and metabolism, redox reactions, circadian rhythms, immune responses and inflammation, DNA repair, cell differentiation, protein signaling, chromatin, and epigenetics. Restoration of NAD + levels in older or diseased animals can promote health and prolong life. Nicotinamide Mononucleotide (NMN) is a biosynthetic precursor of nicotinamide adenine dinucleotide (NAD +) that promotes cellular NAD + production and counteracts age-related pathologies associated with a decline in tissue NAD + levels. NMN acts mainly by converting it into NAD +, which is also known as coenzyme I, and is known as nicotinamide adenine dinucleotide, which is widely distributed in all cells of the human body, participates in thousands of biocatalytic reactions, and is an essential coenzyme in the human body. The decline of NAD + during aging is considered to be a major cause of disease and disability, such as hearing and vision loss, cognitive and motor dysfunction, immune deficiency, arthritis due to autoimmune inflammatory response disorder, metabolic disorders, and cardiovascular disease.

Therefore, NMN supplementation increases the NAD + content in vivo, thereby delaying, ameliorating, and preventing various aging-related manifestations, or age-induced metabolic disorders and geriatric diseases. However, the NMN raw material has an irritating feeling on the digestive system, the taste is poor, dogs and cats cannot take the NMN raw material actively, and the NMN raw material can be taken effectively by supplementing the NMN raw material only by improving the taste through a certain preparation process. In addition, most of NMN similar element B group elements are bitter, and are extremely dislike for dogs and cats.

The tastes of raw materials such as NMN and B group liquid, trace elements and the like are disliked by dogs and cats, active diet for dogs and cats can not be done, dogs and dogs are developed in taste and can taste various tastes such as bitter taste, cats can not eat sweet taste, but cats are particularly sensitive to bitter taste, 12 genes related to bitter taste perception and up to 7 bitter taste receptors can be found through DNA analysis of domestic cats, and cats can recognize harmful substances by means of bitter taste, which means that the risks of poisoning caused by eating poisonous plants and immature fruits by mistake can be avoided in the mode. I.e., NMN, group B liquids and trace elements are not favored by cats and dogs. NMN does not have a market pet product at present, and the existing B group and trace element liquid sold in the market has poor palatability and is difficult to feed.

In patent CN 110463848A, the main phosphate belongs to an acidulant, has strong palatability and fragrant taste, but can cause certain harm to the health of pets after long-term use. Experiments have shown that excessive phosphate consumption aggravates the decline of the renal function and the risk of early death of the rats. Patent CN 111034983A discloses a preparation for chicken flavor using chicken enzymatic hydrolysate and oxidized chicken fat. The product containing the chicken meat enzymolysis liquid and the oxidized chicken fat has short shelf life, generally 12 months, is not used for pets, and is improved aiming at the defects of a phosphate cat phagostimulant and a yeast extract seasoning with chicken meat flavor, the patent CN 109161441A prepares fruit flavor and sweet flavor commonly used for cigarettes through the Maillard reaction of high fructose corn syrup, and the development ideas and the problems of the meat flavor and the pet palatability are two different, and the contrast is completely absent.

Disclosure of Invention

The invention aims to provide an NMN/vitamin B group and trace element efficacy composition with good pet palatability and a preparation method thereof, and solves one or more of the above problems in the prior art.

On one hand, the NMN/vitamin B group and trace element efficacy composition with good pet palatability provided by the invention comprises the following components:

the functional components comprise: NMN, vitamin B group and trace elements;

a major component comprising: high fructose corn syrup, amino acid raw material and yeast extract 708.

In certain embodiments, the vitamin B group comprises one or more of vitamin B1, vitamin B2, vitamin B6, vitamin B12.

In certain embodiments, the amino acid based starting material comprises one or more of glycine, alanine, threonine, lysine, DL-methionine.

In certain embodiments, the NMN/B-vitamin and trace element efficacy composition for pets with good palatability comprises the following components in parts by weight:

wherein: the grape pulp is starch sugar crystal prepared by hydrolyzing and isomerizing plant starch, has sweet taste, and mainly provides monosaccharide for Maillard reaction in the invention;

the flavored yeast extract is a natural flavoring material which is rich in meat flavor substances and is mainly used as a nutrition freshener in the invention, wherein the flavored yeast extract is prepared by taking a yeast extract as a base material and performing thermal degradation of amino acid/peptide, sugar degradation, nucleotide degradation, maillard reaction and the like at high temperature;

glycine has acidic and alkaline functional groups in molecules, has caramel taste, can alleviate acid and alkaline taste, cover bitter taste of saccharin added in food and enhance sweet taste;

alanine has a sweet caramel taste;

threonine has a chocolate taste;

lysine has a fresh bread flavor;

DL-methionine has roasted potato flavor;

the vitamin B1 can generate meat fragrance and can also be used as a catalyst;

vitamin C has antioxidant effect;

zinc gluconate can provide zinc element, wherein zinc is involved in cystine and acid mucopolysaccharide metabolism, and zinc-deficient skin is easy to keratinize;

the sodium benzoate and the sodium sorbate have the function of corrosion prevention;

the xanthan gum has thickening and stabilizing effects;

manganese sulfate can provide manganese element, and manganese can participate in the cell renewal process, and has certain influence on the color and luster of hair;

copper sulfate can provide copper element, and copper is a trace nutrient indispensable to human health, and has important influence on the development and functions of blood, central nervous system, immune system, hair, skin, bone tissue, brain, liver, heart and other internal organs.

on the other hand, the preparation method of the NMN/vitamin B group and trace element efficacy composition with good pet palatability provided by the invention comprises the following steps:

s1, quantitatively weighing qualified raw materials according to a formula;

s2, mixing glycine, alanine and threonine according to a formula ratio to prepare a first premix;

s3, mixing the lysine and the DL-methionine according to the formula ratio to prepare a second premix;

s4, mixing xanthan gum with the second premix to prepare a third premix;

s5, mixing NMN, vitamin B1, vitamin B2, nicotinic acid, pantothenic acid, vitamin B6, biotin, folic acid, vitamin B12, manganese sulfate and copper sulfate to prepare a fourth premix;

s6, mixing water, high fructose corn syrup, the yeast extract 708, a first premix, vitamin B1, vitamin C, zinc gluconate and water to prepare a first reaction solution;

s7, adding the third premix into the first reaction solution, and mixing to prepare a second reaction solution;

s8, adding sodium benzoate and potassium sorbate into the second reaction solution, and mixing to obtain a third reaction solution;

and S9, adding the fourth premix into the third reaction solution, and homogenizing and emulsifying to obtain a mixture.

Wherein: the invention mainly solves the problems of liquid palatability of NMN and certain vitamin and mineral nutritional supplements and long-term stability of a nutrient element liquid preparation through a Maillard reaction, and the composition prepared through the Maillard reaction has rich aroma and bright fruit aroma and sweet aroma.

The maillard reaction refers to the reaction of a monosaccharide (carbonyl) with an amino acid (amino). The extent of the maillard reaction is related to temperature, time, components in the system, water activity, and pH.

In certain embodiments, step S6 is specifically: adding water, high fructose corn syrup, yeast extract 708, a first premix, vitamin B1, vitamin C, zinc gluconate and water into a high-shear homogenizing emulsification tank, starting wall scraping and stirring, and setting the stirring speed to be 40r/min; starting a homogenizing stirring button, and setting the stirring speed to be 3000-4500r/min; simultaneously starting a heating button of the emulsification tank to heat, raising the temperature to 95 ℃, and preserving the heat at 95-100 ℃.

In certain embodiments, the mixing temperature in step S7 is 95-100 ℃ and the mixing time is 30min;

stopping heating during mixing in the step S8;

step S9 specifically includes: when the temperature of the third reaction solution is reduced to 70-80 ℃, adding the fourth premix into the third reaction solution, stopping heating, and continuing to homogenize and emulsify at 1500-2500 r/min.

In certain embodiments, the method of making the NMN/B-vitamin and trace element efficacy composition for pets with good palatability further comprises:

when the temperature of the mixture is reduced to 60 ℃, the mixture is filtered by a 1 mu m screen and then is filled, so that the NMN/vitamin B group and trace element functional composition with good palatability for pets is obtained.

In certain embodiments, the mass ratio of glycine, alanine, threonine in the first premix is 10;

the mass ratio of lysine to DL-methionine in the second premix is 3:1.

Wherein: the present invention divides the amino acid combination into a first amino acid combination participating in the reaction and a second amino acid combination participating in the reaction, i.e., a first premix and a second premix. The first combination of amino acids involved in the reaction is: glycine: alanine: threonine =10:3:5, and must be the first addition of amino acid combination. The second combination of amino acids involved in the reaction is: lysine: methionine =3:1 and must be the addition of a second amino acid combination. The amino acid is added in two batches, and the product which is preferred by the pet can be prepared by controlling the adding time of the amino acid. If not so added, the resulting tasting pet is not as great in palatability and likeability.

Has the advantages that: according to the invention, through the improvement of the palatability of NMN, vitamin B group and trace element liquid, the high fructose corn syrup, the amino acid raw material and the yeast extract 708 raw material are utilized to prepare the pet skin and hair nutrition supplementing micromolecule liquid preparation which has a certain meat flavor, rich meat quality, can well cover the unpleasant odor of the vitamin B group and the trace element, has the stability at normal temperature of 18 months and is easy to absorb.

Detailed Description

The present invention will be described in further detail below with reference to embodiments.

Example 1

An NMN/vitamin B group and trace element efficacy composition with good pet palatability comprising the following components:

the preparation method of the NMN/vitamin B group and trace element efficacy composition with good palatability for pets comprises the following steps:

s1, quantitatively weighing and detecting qualified raw materials according to a formula;

s2, premixing glycine, alanine and threonine according to the formula amount to obtain a first premix;

s3, premixing lysine and DL-methionine according to the formula amount to obtain a second premix;

s4, fully mixing xanthan gum with the second premix to obtain a third premix;

s5, fully mixing NMN, vitamin B1, vitamin B2, nicotinic acid, pantothenic acid, vitamin B6, biotin, folic acid, vitamin B12, manganese sulfate and copper sulfate to obtain a fourth premix;

s6, adding water, high fructose corn syrup, the yeast extract 708, the first premix, B1 (thiamine), vitamin C, zinc gluconate and water into a high-shear homogenizing emulsification tank. Starting a wall scraping stirring button (the stirring speed is set to be 40 r/min) and a homogenizing stirring button (the stirring speed is set to be 3000-4500 r/min), starting a heating button of an emulsifying tank to start heating, raising the temperature to 95 ℃, and preserving the temperature at 95-100 ℃ to prepare a first reaction solution;

s7, when the temperature of the first reaction solution reaches 95 ℃, adding a third premix into the first reaction solution, and preserving the heat at 95-100 ℃ for 30min to prepare a second reaction solution;

s8, keeping the temperature of the second reaction solution for 30min, adding sodium benzoate and potassium sorbate, and stopping heating to obtain a third reaction solution;

s9, when the temperature of the third reaction solution is reduced to 70-80 ℃, adding the fourth premix into the reaction solution, stopping heating, and continuing to perform homogenization at 1500-2500r/min to obtain a mixture;

and S10, uniformly mixing the mixture, filtering the material with a 1um screen when the temperature of the material is reduced to 60 ℃, and filling.

Example 2

An NMN/vitamin B group and trace element efficacy composition with good pet palatability comprises the following components:

s3, premixing the lysine and the DL-methionine according to the formula amount to obtain a second premix;

s4, fully mixing xanthan gum with the second premix to obtain a third premix;

s6, adding water, high fructose corn syrup, the yeast extract 708, the first premix, B1 (thiamine), vitamin C, zinc gluconate and water into a high-shear homogenizing emulsification tank. Starting a wall scraping stirring button (the set stirring speed is 40 r/min) and a homogenizing stirring button (the set stirring speed is 3000-4500 r/min), starting a heating button of an emulsifying tank to start heating, raising the temperature to 95 ℃, and preserving the heat at 95-100 ℃ to prepare a first reaction solution;

s7, adding a third premix into the first reaction solution when the temperature of the first reaction solution reaches 95 ℃, and preserving the heat at 95-100 ℃ for 30min to prepare a second reaction solution;

s8, after the second reaction solution is kept warm for 30min, adding sodium benzoate and potassium sorbate, and stopping heating to prepare a third reaction solution;

Example 3

s4, fully mixing xanthan gum with the second premix to obtain a third premix;

Comparative example 1

DL-methionine was removed from the formulation of example 1 and the other parameters were in accordance with example 1.

Comparative example 2

Lysine and DL-methionine were removed based on the formulation of example 1 and the other parameters were in accordance with example 1.

Comparative example 3

Glycine, alanine and threonine were removed on the basis of the formulation of example 1, and the other parameters were identical to those of example 1.

Comparative example 4

Glycine and alanine were removed from the formulation of example 1 and the other parameters were consistent with example 1.

Performance test

1. Sensory evaluation was performed on example 1 and comparative examples 1 to 4, respectively, and the sensory evaluation table is shown in table 1:

TABLE 1

The sensory evaluation results are shown in table 2:

TABLE 2

Item	Taste of the product	Smell(s)	Palatability for pets	Total score
					Example 1	17	26	47.5	90.5
Comparative example 1	15	22	40	77
					Comparative example 2	10	21	36	67
Comparative example 3	9	18	28	55
					Comparative example 4	11	19	32	62

As can be seen from table 2, the NMN/B vitamin group and trace element effect compositions of example 1 of the present invention, which had better pet palatability than comparative examples 1-4, were of first order in taste, odor and pet palatability. The taste is enough, the meat texture is rich, and no bitter taste exists; the meat flavor is strong, and the taste is pure; meanwhile, more than 80% of pets like to eat. It is obvious that glycine, alanine, threonine, lysine and DL-methionine have important influence on the performance of the final product, and the five amino acids are synergistic and interactive, and are not simple process superposition. Only with the simultaneous addition of these five amino acids will the final composition product be optimal. The main reasons are that: the human smells may not vary significantly, but the pet olfactory system is sensitive, depending on the amount of food intake and the level of liking that the pet is willing to take.

2. Palatability test

Family dogs and cats were selected for testing, 50 small dogs, 50 medium dogs, 50 large dogs, and 50 cats, and palatability testing was performed on the NMN/vitamin B group and trace element efficacy composition of example 1 with good palatability to pets, with results shown in table 3:

TABLE 3

As can be seen from table 3, the higher ratio of NMN/B vitamin and trace element efficacy compositions for dogs and cats fed the pet of the present invention, indicating better palatability of the composition.

3. Mouth resistance test

Preferably, 20 small dogs, 20 medium dogs, 20 large dogs, and 20 cats in a kennel house are selected for oral tolerance testing for 15 days of the NMN/vitamin B group and trace element efficacy composition of example 1 for pet palatability, with results as shown in table 4:

TABLE 4

As can be seen from table 4, the probability that the NMN/vitamin B group and trace element effect composition having good palatability to pets of the invention will be eaten daily by dogs and cats is significantly better than the commercial products (the overall palatability of the marketed B group liquid product, dog and cat, is below 80%), indicating that the composition has better mouth resistance.

4. Hair test

After 10 puppies of 2-3 years old are selected for testing, after the pet eating the NMN/vitamin B group and trace element efficacy composition with good palatability provided by the invention in 1,1-3 months, the pet basically has no hair problem, the hair luster is good, and the fluff Mao Maocheng has no hair loss phenomenon.

5. Accelerated stability test

The NMN/vitamin B group and trace element efficacy composition with good pet palatability in example 1 of the invention is selected for accelerated stability study, and the accelerated condition is a stability test box with 40 ℃ and 75% humidity. The stability test observations were made at 0 day, 15 days, 1 month, 2 months and 3 months, respectively, and the results are shown in table 5:

TABLE 5

As can be seen from Table 5, the NMN/vitamin B group and trace element efficacy composition with good palatability for pets has good stability, and the product performance basically has no change within 0-3 months, which shows that the preparation method and the synergistic effect among the raw material components are beneficial to improving the stability of the composition.

In conclusion: the invention has the following advantages:

1. NMN, vitamin B group and trace element raw materials in the prior art have poor taste, are sour and bitter to some extent, and are unpleasant taste for pet cats and dogs, and the invention improves the bad taste of liquid solution through natural nutrient elements, thereby effectively achieving the effect of attracting cats and dogs to eat actively;

2. the food calling effect of the patent is that a product with a pet food calling effect is prepared without adding harmful phosphate with a strong dog and cat food calling effect;

3. the meat flavor is not generated by adding natural meat and animal fat which have poor quality guarantee period and are easy to cause obesity to dogs and cats;

4. the high fructose corn syrup, the amino acid raw material and the yeast extract 708 raw material are utilized to generate a stable and easily absorbed pet NMN/B vitamin and trace element nutrition supplementing micromolecule liquid preparation which has a certain strong meat flavor and rich meat quality and can well cooperate with the bad smell of NMN/B vitamin and trace element under the control of a certain temperature and time;

5. the liquid preparation with micron-sized liquid molecules, quick absorption, doubled effect and formulation of the formula can be stably stored for 18 months by high-speed homogenizing and emulsifying equipment.

The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various changes and modifications can be made without departing from the inventive concept of the present invention, and these should be considered as within the scope of the present invention.

Claims

1. The flavoring agent with good palatability for pets is characterized by being prepared by two Maillard reactions, wherein the raw materials of the first Maillard reaction comprise water, high fructose syrup, yeast extract 708, glycine, alanine, threonine, vitamin B1, vitamin C and zinc gluconate; on the basis of the first Maillard reaction, the raw materials added in the second Maillard reaction comprise lysine, DL-methionine, xanthan gum, sodium benzoate and potassium sorbate.

2. The flavoring agent according to claim 1, wherein the Maillard reaction main materials comprise the following components in parts by weight:

3. the flavoring agent according to claim 2, wherein the weight ratio of glycine, alanine and threonine is 10.

4. The flavor of claim 2 wherein the first Maillard reaction step is:

s1, quantitatively weighing qualified raw materials according to a formula;

s3, mixing water, high fructose corn syrup, the yeast extract 708, a first premix, vitamin B1, vitamin C, zinc gluconate and water to prepare a first reaction solution;

s4, adding the first reaction solution into a high-shear homogeneous emulsification tank, starting a scraping wall to stir, and setting the stirring speed to be 40r/min; starting a homogenizing stirring button, and setting the stirring speed to be 3000-4500r/min; and simultaneously starting a heating button of the emulsifying tank to heat, raising the temperature to 95 ℃, and preserving the heat at 95-100 ℃ to obtain a first Maillard reaction product.

5. The flavoring agent according to claim 4, wherein the second Maillard reaction step is:

s5, mixing the lysine and the DL-methionine according to the formula ratio to prepare a second premix;

s7, mixing xanthan gum with the second premix according to the formula amount to prepare a third premix;

and S8, adding the third premix into the first Maillard reaction product, controlling the temperature to be 95-100 ℃, mixing for 30min, stopping heating, and adding sodium benzoate and potassium sorbate according to the formula amount.

6. An NMN/vitamin B group and trace element efficacy composition with good pet palatability, comprising the following components:

the functional components comprise: NMN, vitamin B group and trace elements;

and the flavor agent of claim 4.

7. The NMN/B-vitamin efficacy composition for pets according to claim 6, wherein said B-vitamin comprises one or more of B1, B2, B6, B12 vitamins and said trace elements comprise one or more of zinc, manganese, copper.

8. The NMN/vitamin B group and trace element efficacy composition for pets with good palatability according to claim 7, wherein the parts by weight of the efficacy ingredients are:

9. the NMN/vitamin B group and trace element efficacy composition for pets with good palatability according to claim 6, wherein the addition steps of the efficacy ingredients are as follows:

s9, mixing NMN, vitamin B1, vitamin B2, nicotinic acid, pantothenic acid, vitamin B6, biotin, folic acid, vitamin B12, manganese sulfate and copper sulfate to prepare a fourth premix;

and S10, when the temperature of the product of the second Maillard reaction is reduced to 70-80 ℃, adding the fourth premix, and continuing to perform homogenization at 1500-2500 r/min.

10. The NMN/VB and trace element efficacy composition with good palatability for pets according to claim 8, wherein when the S10 homogeneous emulsification temperature is reduced to 60 ℃, the materials are filtered by a 1 μm screen and filled, so that the NMN/VB and trace element efficacy composition with good palatability for pets is obtained.