CN115382671A - Rapid pretreatment device for nucleic acid amplification - Google Patents
Rapid pretreatment device for nucleic acid amplification Download PDFInfo
- Publication number
- CN115382671A CN115382671A CN202211022411.1A CN202211022411A CN115382671A CN 115382671 A CN115382671 A CN 115382671A CN 202211022411 A CN202211022411 A CN 202211022411A CN 115382671 A CN115382671 A CN 115382671A
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- retainer
- nucleic acid
- fixedly connected
- acid amplification
- pretreatment device
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 42
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 40
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 40
- 230000003321 amplification Effects 0.000 title claims abstract description 23
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 23
- 238000001179 sorption measurement Methods 0.000 claims abstract description 16
- 230000017525 heat dissipation Effects 0.000 claims abstract description 11
- 238000001514 detection method Methods 0.000 abstract description 12
- 238000005119 centrifugation Methods 0.000 abstract description 5
- 230000003111 delayed effect Effects 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 8
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000004804 winding Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000003761 preservation solution Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B15/00—Other accessories for centrifuges
- B04B15/02—Other accessories for centrifuges for cooling, heating, or heat insulating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B9/00—Drives specially designed for centrifuges; Arrangement or disposition of transmission gearing; Suspending or balancing rotary bowls
- B04B9/10—Control of the drive; Speed regulating
Landscapes
- Centrifugal Separators (AREA)
Abstract
The invention provides a rapid pretreatment device for nucleic acid amplification, which belongs to the technical field of medical instruments and aims to solve the problems that a retainer can continuously rotate for a period of time under the action of inertia after the centrifugation of the existing centrifugal machine is finished, so that a sample cannot be taken out in time and the working progress is delayed; the heat dissipation holes are formed in the lower end face of the centrifuge main body; the driving piece is fixedly connected inside the lower end of the centrifuge main body; the fixed plate is fixedly connected to the middle part of the inner side of the centrifuge main body; the adaptor is coaxially and fixedly connected with the driving piece; the retainer is inserted at the upper end of the adapter; the adsorption piece is connected to the inner side of the fixing plate in a sliding manner; the invention can effectively reduce the time of the inertial rotation of the retainer, thereby ensuring that the sample can be taken out more quickly, ensuring the accuracy of the subsequent nucleic acid amplification detection result and avoiding the situation of wrong and repeated detection of the result.
Description
Technical Field
The invention belongs to the technical field of medical instruments, and particularly relates to a rapid pretreatment device for nucleic acid amplification.
Background
The nucleic acid amplification detection is a method for amplifying a nucleic acid sequence to be detected through the action of enzyme and then detecting, wherein medical staff preprocesses a collected nucleic acid sample (fully and uniformly agitates a swab in a preservation solution through a centrifugal machine), then extracts supernate to extract nucleic acid, and performs a series of analyses on the extracted nucleic acid to obtain the nucleic acid sequence so as to realize nucleic acid amplification detection, wherein the time and the position of sample collection, the treatment and the preservation of the sample, the efficiency of nucleic acid extraction and the sensitivity of an amplification reagent can influence the final result.
For example CN201410803133.2 discloses a centrifuge comprising: a rotor; a rotor chamber having an opening; an electric motor; a door configured to close the opening; an input/output unit configured to receive an input of an operation condition and display an operation state; and a housing that accommodates the rotor, the rotor chamber, the motor, the door, and the input/output unit, wherein an upper surface of the housing has the opening, a light-emitting portion is provided near a corner where the upper surface and the front surface of the housing intersect each other or near a front side of the opening, a horizontal width of the light-emitting portion is longer than the opening, and a light-emitting form of the light-emitting portion is configured to be changed based on an operation state of the centrifuge.
Based on the aforesaid, current centrifuge usually comprises rotor, motor, holder etc. put the sample in the holder when using, drive holder and sample through the motor and rotate at a high speed and realize the centrifugal treatment to the nucleic acid sample, but after centrifugation the holder can continue to rotate a period under the inertia effect, lead to the sample can not in time take out, delay the job schedule, and if directly can make the sample take place great shock again according to stopping the holder, lead to the precipitate in the sample to float and influence centrifugal effect, influence subsequent testing result.
Therefore, in view of the above, the present invention provides a rapid pretreatment device for nucleic acid amplification, which is improved based on the existing structure and defects, so as to achieve the purpose of higher practical value.
Disclosure of Invention
In order to solve the technical problems, the invention provides a rapid pretreatment device for nucleic acid amplification, which aims to solve the problems that the conventional centrifugal machine usually comprises a rotor, a motor, a retainer and the like, when the centrifugal machine is used, a sample is placed in the retainer, the retainer and the sample are driven by the motor to rotate at a high speed to realize centrifugal treatment on the nucleic acid sample, but after centrifugation is finished, the retainer can continuously rotate for a period of time under the action of inertia, so that the sample cannot be taken out in time, the working progress is delayed, and if the retainer is directly pressed and stopped, the sample can generate larger vibration, so that precipitates in the sample float, the centrifugal effect is influenced, and the subsequent detection result is influenced.
The invention relates to a rapid pretreatment device for nucleic acid amplification, which has the purpose and the effect and is achieved by the following specific technical means:
a rapid pretreatment device for nucleic acid amplification comprises a centrifuge body, a heat dissipation hole, a driving piece, a fixing plate, a retainer, an adapter and an adsorption piece; the plurality of heat dissipation holes are uniformly distributed and arranged on the lower end face of the centrifuge body; the driving piece is fixedly connected inside the lower end of the centrifuge main body; the fixed plate is fixedly connected to the middle part of the inner side of the centrifuge main body; the adaptor is coaxially and fixedly connected with the driving piece; the retainer is inserted at the upper end of the adapter; six adsorption pieces are arranged, and the six adsorption pieces are connected to the inner side of the fixing plate in a circumferential array sliding mode.
Further, the retainer comprises a pull ring; the pull ring is fixedly connected to the upper end face of the retainer.
Furthermore, the retainer also comprises a connecting hole; the connecting hole is formed in the lower end face of the retainer;
the adaptor comprises a connecting shaft; the connecting shaft is fixedly connected to the upper end face of the adapter piece and is in sliding connection with the connecting hole.
Furthermore, the fixing plate comprises an electromagnet and a mounting groove; the mounting groove is formed in the outer side of the fixing plate; the electromagnet is circular, and the electromagnet is fixedly connected to the outer side of the mounting groove.
Furthermore, the fixing plate also comprises a sliding groove; six sliding grooves are arranged on the inner side of the fixed plate in a circumferential array manner;
the adsorption piece comprises a deceleration soft brush; the soft brush of speed reduction is provided with six, and six soft brushes of speed reduction are fixed connection respectively on six medial surfaces that adsorb the piece.
Furthermore, the adsorption piece also comprises an elastic piece; and each speed-reducing soft brush is provided with three elastic pieces, and the six speed-reducing soft brushes are respectively and elastically connected to the inner sides of the six sliding grooves through the elastic pieces.
Compared with the prior art, the invention has the following beneficial effects:
the invention adds the deceleration soft brush on the basis of the traditional centrifuge, controls the position change of the deceleration soft brush through the electromagnet, when the centrifuge is electrified to work, the electromagnet is used for enabling the adsorption part to move outwards to be attached to the electromagnet, so that the deceleration soft brush is far away from the retainer, the normal state of the centrifuge is ensured, after the centrifuge finishes working, the electromagnet is demagnetized, the deceleration soft brush rapidly and automatically rebounds under the action of the elastic part to realize the deceleration of the retainer, the deceleration soft brush is attached to the retainer through the deceleration soft brush, so that the friction force is generated between the deceleration soft brush and the retainer, the resistance force applied when the retainer rotates inertially is increased, the retainer stops more rapidly, the time of the inertial rotation of the retainer is effectively reduced, the sample can be taken out more rapidly, the working efficiency is improved, the working progress is improved, the condition that the sample vibrates due to the fact that the retainer is directly pressed to stop is avoided, the centrifugal effect of the sample is ensured, the accuracy of the subsequent nucleic acid amplification detection result is effectively ensured, the condition that the repeated detection is needed after the result is wrong is avoided, the labor intensity of the workers is effectively reduced, and the working efficiency of the workers is improved.
According to the invention, a fixed retainer on the traditional centrifuge is changed into a detachable retainer, the replacement of the retainer is facilitated through the connecting hole and the connecting shaft, the replacement speed of a nucleic acid sample to be treated is increased by replacing the retainer, so that the working efficiency is improved, the pretreatment speed of the nucleic acid sample is increased, the retainer can be conveniently taken through the pull ring, and the replacement efficiency of the retainer is improved.
The invention can effectively reduce the time of the inertial rotation of the retainer, thereby ensuring that the sample can be taken out faster, improving the working efficiency and the working progress, simultaneously avoiding the condition that the sample vibrates because the retainer is directly pressed and stopped, ensuring the centrifugal effect of the sample, effectively ensuring the accuracy of the subsequent nucleic acid amplification detection result, avoiding the condition that the result needs to be detected repeatedly after being wrong, effectively reducing the labor intensity of workers, thereby improving the working efficiency of the workers, accelerating the replacement speed of the nucleic acid sample to be processed, improving the working efficiency and improving the speed of the pretreatment of the nucleic acid sample.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is a schematic view of the fixing plate structure of the present invention.
Fig. 3 is a schematic view of the structure of the sliding groove of the present invention.
Fig. 4 is a schematic view of the structure of the decelerating soft brush of the invention.
Fig. 5 is a schematic cross-sectional structural view of the present invention.
In the drawings, the corresponding relationship between the component names and the reference numbers is as follows:
1. a centrifuge body; 2. heat dissipation holes; 3. a drive member; 4. a fixing plate; 401. an electromagnet; 402. mounting grooves; 403. a sliding groove; 5. a cage; 501. a pull ring; 502. connecting holes; 6. an adapter; 601. a connecting shaft; 7. an adsorbing member; 701. a deceleration soft brush; 702. an elastic member.
Detailed Description
Embodiments of the present invention will be described in further detail with reference to the drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and to simplify the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless explicitly stated or limited otherwise, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Example (b):
as shown in figures 1 to 5:
the invention provides a rapid pretreatment device for nucleic acid amplification, which comprises a centrifuge body 1, a heat dissipation hole 2, a driving piece 3, a fixing plate 4, a retainer 5, an adapter piece 6 and an adsorption piece 7; a plurality of heat dissipation holes 2 are arranged, and the heat dissipation holes 2 are uniformly distributed on the lower end surface of the centrifuge main body 1; the driving piece 3 is fixedly connected inside the lower end of the centrifuge main body 1; the fixed plate 4 is fixedly connected to the middle part of the inner side of the centrifuge body 1; the adaptor 6 is coaxially and fixedly connected with the driving piece 3; the retainer 5 is inserted at the upper end of the adapter 6; six suction members 7 are arranged, and six suction members 7 are connected to the inner side of the fixing plate 4 in a sliding mode in a circumferential array mode.
Wherein, the fixed plate 4 comprises an electromagnet 401 and a mounting groove 402; the mounting groove 402 is arranged at the outer side of the fixing plate 4; the electromagnet 401 is annular, and the electromagnet 401 is fixedly connected to the outer side of the mounting groove 402; in use, make centrifuge circular telegram work through driving piece 3, centrifuge produces magnetic force through electro-magnet 401 after circular telegram, electro-magnet 401 is at the outside winding of iron core rather than power assorted conductive winding and constitutes, this kind of coil that leads to the electric current has magnetism like magnet, the outage can be demagnetized immediately, make adsorbing piece 7 remove laminating electro-magnet 401 to the outside through electro-magnet 401, make the soft brush 701 that slows down keep away from holder 5, make holder 5 drive nucleic acid sample carry out high-speed rotation and realize centrifugation, accomplish the preliminary treatment of nucleic acid sample, prevent effectively that soft brush 701 that slows down from interfering centrifuge's normal work, guarantee centrifuge's work efficiency, discharge centrifuge with the heat that driving piece 3 during operation gived off through louvre 2, effectively cool down for driving piece 3.
Wherein, the retainer 5 comprises a pull ring 501; the pull ring 501 is fixedly connected to the upper end surface of the retainer 5; in use, the retainer 5 can be taken conveniently through the pull ring 501, and the replacement efficiency of the retainer 5 is improved.
Wherein, the retainer 5 also comprises a connecting hole 502; the connecting hole 502 is arranged on the lower end surface of the retainer 5;
the adaptor 6 comprises a connecting shaft 601; the connecting shaft 601 is fixedly connected to the upper end face of the adapter 6, and the connecting shaft 601 is connected with the connecting hole 502 in a sliding mode; in use, the holder 5 is conveniently replaced through the connecting hole 502 and the connecting shaft 601, and the replacement speed of the nucleic acid sample to be processed is increased by replacing the holder 5, so that the working efficiency is improved, and the speed of nucleic acid sample pretreatment is increased.
Wherein, the fixing plate 4 further comprises a sliding groove 403; six sliding grooves 403 are arranged, and six sliding grooves 403 are circumferentially arrayed on the inner side of the fixed plate 4;
the adsorption member 7 comprises a deceleration soft brush 701; six deceleration soft brushes 701 are arranged, and the six deceleration soft brushes 701 are respectively and fixedly connected to the inner side surfaces of the six adsorption pieces 7; in use, the speed-reducing soft brush 701 can move through the sliding groove 403, after the centrifugal machine finishes working, the speed-reducing soft brush 701 is attached to the retainer 5, the resistance force applied when the retainer 5 rotates inertially is increased through the friction force generated between the speed-reducing soft brush 701 and the retainer 5, the retainer 5 is stopped more quickly, the time of the inertial rotation of the retainer 5 is effectively reduced, so that a sample can be taken out more quickly, the working efficiency is improved, the working progress is improved, the condition that the sample vibrates due to the fact that the retainer 5 is directly stopped is avoided, the centrifugal effect of the sample is ensured, the accuracy of a subsequent nucleic acid amplification detection result is effectively ensured, the condition that repeated detection is needed after a result error is avoided, the labor intensity of workers is effectively reduced, and the working efficiency of the workers is improved.
Wherein, the absorption member 7 further comprises an elastic member 702; each decelerating soft brush 701 is provided with three elastic members 702, and the six decelerating soft brushes 701 are respectively and elastically connected to the inner sides of the six sliding grooves 403 through the elastic members 702; in use, after the electromagnet 401 is demagnetized, the decelerating soft brush 701 rapidly and automatically rebounds under the action of the elastic piece 702, so that the retainer 5 is decelerated.
The specific use mode and function of the embodiment are as follows: in use, the centrifuge is powered on by the driving part 3 to work, the centrifuge generates magnetic force by the electromagnet 401 after being powered on, the adsorbing part 7 moves outwards by the electromagnet 401 to be attached to the electromagnet 401, the speed reducing soft brush 701 is far away from the holder 5, the holder 5 drives the nucleic acid sample to rotate at high speed to realize centrifugation, the pretreatment of the nucleic acid sample is completed, the speed reducing soft brush 701 is effectively prevented from interfering the normal work of the centrifuge, the work efficiency of the centrifuge is ensured, the heat emitted by the driving part 3 during the work is discharged out of the centrifuge through the heat dissipation holes 2, the temperature of the driving part 3 is effectively reduced, after the centrifuge finishes the work, the electromagnet 401 is demagnetized, the speed reducing soft brush 701 rebounds rapidly and automatically under the action of the elastic part 702 to realize the speed reduction of the holder 5, the speed reducing soft brush 701 is attached to the holder 5, so that friction force is generated between the speed reducing soft brush 701 and the holder 5, thereby increasing the resistance of the retainer 5 when the retainer 5 rotates inertially, leading the retainer 5 to stop more quickly, effectively reducing the time of the inertial rotation of the retainer 5, thereby ensuring that the sample can be taken out more quickly, improving the working efficiency, improving the working progress, simultaneously avoiding the condition that the sample vibrates because the retainer 5 is directly pressed to stop, ensuring the centrifugal effect of the sample, effectively ensuring the accuracy of the subsequent nucleic acid amplification detection result, avoiding the condition that the detection needs to be repeated after the result is wrong, effectively reducing the labor intensity of workers, thereby improving the working efficiency of the workers, facilitating the replacement of the retainer 5 through the connecting hole 502 and the connecting shaft 601, accelerating the replacement speed of the nucleic acid sample to be processed by replacing the retainer 5, thereby improving the working efficiency, improving the speed of the pretreatment of the nucleic acid sample, and facilitating the taking of the retainer 5 through the pull ring 501, the replacement efficiency of the retainer 5 is improved.
The embodiments of the present invention have been presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the invention in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiment was chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated.
Claims (6)
1. A rapid pretreatment device for nucleic acid amplification, characterized in that: the centrifugal machine comprises a centrifugal machine body (1), heat dissipation holes (2), a driving piece (3), a fixing plate (4), a retainer (5), an adapter piece (6) and an adsorption piece (7); the plurality of heat dissipation holes (2) are arranged, and the heat dissipation holes (2) are uniformly distributed and arranged on the lower end face of the centrifuge main body (1); the driving piece (3) is fixedly connected inside the lower end of the centrifuge main body (1); the fixed plate (4) is fixedly connected to the middle part of the inner side of the centrifuge main body (1); the adaptor (6) is coaxially and fixedly connected with the driving piece (3); the retainer (5) is inserted at the upper end of the adapter (6); six adsorption pieces (7) are arranged, and the six adsorption pieces (7) are connected to the inner side of the fixing plate (4) in a circumferential array sliding mode.
2. A rapid pretreatment device for nucleic acid amplification according to claim 1, wherein: the fixed plate (4) comprises an electromagnet (401) and a mounting groove (402); the mounting groove (402) is formed in the outer side of the fixing plate (4); the electromagnet (401) is annular, and the electromagnet (401) is fixedly connected to the outer side of the mounting groove (402).
3. The rapid pretreatment device for nucleic acid amplification according to claim 1, wherein: the retainer (5) comprises a pull ring (501); the pull ring (501) is fixedly connected to the upper end face of the retainer (5).
4. A rapid pretreatment device for nucleic acid amplification according to claim 1, wherein: the retainer (5) also comprises a connecting hole (502); the connecting hole (502) is formed in the lower end face of the retainer (5);
the adaptor (6) comprises a connecting shaft (601); the connecting shaft (601) is fixedly connected to the upper end face of the adapter (6), and the connecting shaft (601) is connected with the connecting hole (502) in a sliding mode.
5. The rapid pretreatment device for nucleic acid amplification according to claim 1, wherein: the fixed plate (4) also comprises a sliding groove (403); six sliding grooves (403) are arranged, and the six sliding grooves (403) are circumferentially arrayed on the inner side of the fixing plate (4);
the adsorption piece (7) comprises a deceleration soft brush (701); the six speed-reducing soft brushes (701) are arranged, and the six speed-reducing soft brushes (701) are respectively and fixedly connected to the inner side surfaces of the six adsorption pieces (7).
6. A rapid pretreatment device for nucleic acid amplification according to claim 5, wherein: the adsorption piece (7) further comprises an elastic piece (702); each speed-reducing soft brush (701) is provided with three elastic pieces (702), and the six speed-reducing soft brushes (701) are respectively and elastically connected to the inner sides of the six sliding grooves (403) through the elastic pieces (702).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211022411.1A CN115382671B (en) | 2022-08-25 | 2022-08-25 | A quick preprocessing device for nucleic acid amplification |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211022411.1A CN115382671B (en) | 2022-08-25 | 2022-08-25 | A quick preprocessing device for nucleic acid amplification |
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| CN115382671B CN115382671B (en) | 2024-04-02 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115181651A (en) * | 2022-08-12 | 2022-10-14 | 苏州海苗生物科技有限公司 | Optical detection equipment for nucleic acid amplification |
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