CN115181634A - Single cell separation device - Google Patents
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- CN115181634A CN115181634A CN202210728649.XA CN202210728649A CN115181634A CN 115181634 A CN115181634 A CN 115181634A CN 202210728649 A CN202210728649 A CN 202210728649A CN 115181634 A CN115181634 A CN 115181634A
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- 238000000926 separation method Methods 0.000 title claims abstract description 45
- 239000010902 straw Substances 0.000 claims abstract description 23
- 239000012780 transparent material Substances 0.000 claims abstract description 8
- 238000009630 liquid culture Methods 0.000 claims description 34
- 230000002572 peristaltic effect Effects 0.000 claims description 14
- 238000002955 isolation Methods 0.000 claims description 5
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 2
- 238000000638 solvent extraction Methods 0.000 claims description 2
- 230000003321 amplification Effects 0.000 abstract description 4
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000002265 prevention Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 11
- 239000007788 liquid Substances 0.000 description 9
- 238000013461 design Methods 0.000 description 5
- 238000005192 partition Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 2
- 210000000887 face Anatomy 0.000 description 2
- 238000000684 flow cytometry Methods 0.000 description 2
- 230000005389 magnetism Effects 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 241000203069 Archaea Species 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000004720 dielectrophoresis Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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Abstract
The invention relates to a single cell separation device. The technical scheme is as follows: comprises a base, a sterile box, a cell separation mechanism and a drive control mechanism; the base adopts transparent material, and the fluorescent tube is installed respectively to both sides around its lower surface, base upper portion sets up aseptic box, the lower surface threaded connection at aseptic box top portion cell separating mechanism, cell separating mechanism includes T type fixed block, the lower extreme and the elastic spiral line fixed connection of T type fixed block, elastic spiral line's lower extreme fixed connection holding rod, the first L type straw of lower extreme fixed connection of holding rod, the upper portion lateral wall opening fixed connection second L type straw of first L type straw, the lower part pipe wall suit fixed block of first L type straw, the left side wall fixed connection high definition digtal camera of fixed block, the outside lateral wall fixed mounting drive control mechanism in rear side of aseptic box. The invention has the beneficial effects that: aseptic operation, pollution prevention, high-definition amplification search and efficiency improvement.
Description
Technical Field
The invention belongs to the technical field of biochemical experiment devices, and relates to a single cell separation device.
Background
Organisms can be divided into unicellular organisms and multicellular organisms according to the number of cells that constitute them. Unicellular organisms consist of only a single cell and often converge to form a cell colony. The earliest organisms on earth formed approximately thirty-five hundred million years ago to forty-one hundred million years ago, and prokaryotes were the most primitive organisms such as bacteria and blue-green algae and occur in warm water. Unicellular organisms include all archaea and eubacteria and many protists. Because the single structure of the single cell can avoid other interference factors, the single cell becomes an important sample and a unique research object of biochemical research, and when the single cell is researched, how to separate the single cell is the key for researching the single cell.
The cell capture and separation refers to a process of separating one or more cells from a liquid mixed with a plurality of cells by physical, chemical, biological and other means. Cell capture isolation is an extremely important part of modern biology and is also one of the key steps in cell research. Cell capture separation is an important experimental approach in immunology, diagnostic testing and pathology research. The captured and separated cells can be applied to experiments such as cell counting, cell culture and the like. The existing cell capturing and separating methods are various, such as flow cytometry separation, dielectrophoresis separation and the like, but the flow cytometry instrument has the disadvantages of large modeling, complex structure, high cost and expensive machine, needs professional personnel for operation, has high using condition, is not convenient for large-area popularization and use, and has relatively low capturing rate and long-time sample preparation, and in addition, the two methods have great requirements on the sample amount.
For some experimental scenes that the demand of single cells is not very large, the extraction and separation of the single cells are carried out by adopting a micromanipulation method, the existing micromanipulation method needs an experimenter to look at the position of the single cells continuously by a microscope, the eyes of the experimenter are tired due to long-time work, the labor intensity is higher, in addition, a single cell suction device usually adopts a glass suction tube with a simple structure, and the experimenter cannot accurately and quickly extract the single cells from the cell centrifugate, so the single cell separation device provided by the invention adopts a high-definition camera to match with a cell separation mechanism, realizes the visual field amplification search of mixed cell sap, further realizes the quick identification and suction of the single cells, improves the efficiency and the accuracy, and relieves the labor intensity of the experimenter adopting the traditional micromanipulation method.
Disclosure of Invention
In view of the problems in the prior art, the invention discloses a single cell separation device, which adopts the technical scheme that the single cell separation device comprises a base, a sterile box, a cell separation mechanism and a driving control mechanism; the base comprises a tray, the tray is made of transparent materials, a cross partition is arranged in the tray, foot pads are respectively arranged at four corners of the lower surface of the tray, lamp tubes are respectively arranged at the front side and the rear side of the lower surface of the tray, the upper portion of the base is provided with the sterile box, a convex edge is downwards arranged on the inner side wall of the lower end of the sterile box, round holes are formed in the centers of the left side wall and the right side wall of the sterile box, isolating sleeves are respectively arranged in the boxes at the inner edges of the left side and the right side of the round holes, the edge of an opening at the top end of the sterile box is in threaded connection with the edge of a cover, the center of the lower surface of the cover is in threaded connection with the cell separation mechanism, the cell separation mechanism comprises a T-shaped fixed block, the lower end of the T-shaped fixed block is fixedly connected with one end of an elastic spiral line, the lower end of the elastic spiral line is fixedly connected with a holding rod, control buttons are respectively arranged on the upper portion and the lower side wall of the holding rod, the lower end of the holding rod is fixedly connected with a first L-shaped suction tube, the left side wall of the fixed block is fixedly connected with a camera head, and the lower end of the first L-shaped suction tube is fixedly connected with a side wall of the side of the sterile box; through the design of the movable connection between the base and the sterile box, experimenters can add mixed liquid to the tray of the base and clean and maintain the tray; meanwhile, the whole base is wrapped together by the sterile box, so that the sample is prevented from being exposed in the air for too long time during testing to cause pollution; in addition, the designed first L-shaped straw structure is matched with a high-definition camera arranged at the bent pipe of the straw structure, so that effective amplification of single cells can be realized, and the accuracy in the process of suction is improved; finally, the peristaltic pump is adopted as the driving control mechanism, so that the suction force can be effectively controlled, the technical capability requirement of the traditional manual labor hour is reduced, and the universality of the device is improved.
As a preferable scheme of the present invention, the cross partition is arranged to partition the interior of the tray into a first mixed liquid culture dish, a second mixed liquid culture dish, a first separated liquid culture dish and a second separated liquid culture dish; through separating into corresponding culture dish with the tray, can promote the experimenter and after the separation unicellular, place unicellular convenience, reduce frequently to open aseptic box and base simultaneously, cause bacterial contamination's probability.
As a preferable scheme of the present invention, the upper part of the front side lamp tube faces right to the left and right central lines of the first and second separated liquid culture dishes, and the upper part of the rear side lamp tube faces right to the left and right central lines of the first and second mixed liquid culture dishes; by the design, the lower part of the tray can be ensured to irradiate through the lamp tubes arranged in the front and at the back under the dim operating environment of light, and the controllability of the experimental environment is ensured.
As a preferred scheme of the invention, the sterile box is made of transparent materials; the aseptic box adopts transparent material, can ensure that the experimenter is in the in-process of isolation unicellular, and the operating condition of each mechanism of outside visual observation aseptic box inside improves the device's controllability and security.
As a preferred scheme of the invention, a touch screen is installed in a groove on the left side of the upper surface of the cover, and a control panel is installed in a groove on the cover on the front side of the touch screen; through touch-sensitive screen and control panel, can adjust high definition digtal camera and peristaltic pump's operating parameter in a flexible way, the experimental personnel of being convenient for in the test process, nimble operation the device improves the convenience.
As a preferred scheme of the invention, a magnetic suction seat is arranged on the inner side wall of the rear side of the sterile box, and a clamping groove is formed in the magnetic suction seat.
As a preferred scheme of the invention, the magnetic suction seat is matched with the fixed block in a magnetic suction manner through the clamping groove, and the cell separation mechanism is adsorbed on the magnetic suction seat for fixing; through the draw-in groove of magnetism suction seat, can carry out the sample at the experimenter on the way, as required, go into corresponding draw-in groove with cell separating mechanism card in, get in a flexible way and put, perhaps after experimental, go into corresponding draw-in groove with cell separating mechanism card in, prevent the inner wall contact of first L type straw and aseptic tank, cause the damage.
As a preferable scheme of the invention, the driving control mechanism comprises a peristaltic pump, the peristaltic pump is respectively provided with an inlet pipeline and an outlet pipeline, the inlet pipeline and the outlet pipeline respectively penetrate through the rear side wall of the sterile box, and the right side of the peristaltic pump is provided with a controller; design like this, can guarantee that inlet pipeline and outlet pipeline contact with the inside air of aseptic box or cell mixed liquid, prevent because set up outside aseptic box, introduce miscellaneous fungus and cause stained.
As a preferable scheme of the invention, the inlet pipeline is communicated with the free end of the second L-shaped suction pipe through a hose; through adopting the hose with the inlet tube with communicate between the second L type straw, can be convenient for cell separating mechanism nimble shift position in aseptic box, and do not receive the inlet tube with the restriction of being connected between the second L type straw.
The invention has the beneficial effects that:
through the design of the movable connection between the base and the sterile box, experimenters can add mixed liquid to the tray of the base and clean and maintain the tray;
the whole base is wrapped together by the sterile box, so that the sample is prevented from being exposed in the air for too long time during testing to cause pollution;
the cell separating mechanism of design adopts the high definition digtal camera of its return bend department installation of first L type straw structure cooperation, can realize effectively enlargiing to mixed liquid to enlarge the view of searching unicellular, the degree of accuracy when promoting the absorption, drive control mechanism adopts the peristaltic pump, the dynamics that can effective control absorb reduces the technical ability requirement that tradition relied on man-hour, promote the device's commonality, with this experimental efficiency who promotes traditional micro-operation method, alleviate experimenter's intensity of labour.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a cross-sectional view of the overall structure of the present invention;
FIG. 3 is a schematic view of a base of the present invention;
FIG. 4 is a bottom view of the base of the present invention;
FIG. 5 is a cross-sectional view of a sterilization case of the present invention;
FIG. 6 is a schematic view of a cell separation mechanism according to the present invention;
fig. 7 is a schematic view of the drive control mechanism of the present invention.
In the figure: 1-base, 2-aseptic box, 3-cell separation mechanism, 4-drive control mechanism, 11-tray, 12-cross partition, 13-foot pad, 14-lamp tube, 15-first mixed liquid culture dish, 16-second mixed liquid culture dish, 17-first separated liquid culture dish, 18-second separated liquid culture dish, 21-convex edge, 22-round hole, 23-isolation sleeve, 24-cover, 25-touch screen, 26-control panel, 27-magnetic suction seat, 271-clamping groove, 31-T type fixing block, 32-elastic spiral line, 33-holding rod, 331-control button, 34-first L type suction tube, 35-second L type suction tube, 36-fixing block, 37-camera, 41-peristaltic pump, 411-inlet pipeline, 412-outlet pipeline and 42-controller.
Detailed Description
Example 1
As shown in fig. 1 to 7, the single-cell separation device according to the present invention adopts the technical scheme that the single-cell separation device comprises a base 1, a sterile box 2, a cell separation mechanism 3 and a driving control mechanism 4; base 1 includes tray 11, tray 11 adopts transparent material, set up the cross in the tray 11 and cut off 12, callus on the sole 13 is installed respectively to four angles of tray 11 lower surface, fluorescent tube 14 is installed respectively to both sides around the 11 lower surfaces of tray, it will to set up the cross to cut off 12 the internal partitioning of tray 11 is first mixed liquid culture dish 15, the mixed liquid culture dish of second 16, first separation liquid culture dish 17 and second separation liquid culture dish 18, the front side the upper portion of fluorescent tube 14 is just right the central line about first separation liquid culture dish 17, second separation liquid culture dish 18, the rear side the upper portion of fluorescent tube 14 is just right the central line about first mixed liquid culture dish 15, the mixed liquid culture dish 16 of second.
The upper portion of the base 1 is provided with the sterile box 2, the sterile box 2 is made of transparent materials, a convex edge 21 is downwards arranged on the inner side wall of the lower end of the sterile box 2, a round hole 22 is formed in the center of the left side wall and the right side wall of the sterile box 2, an isolation sleeve 23 is respectively arranged in the inner edge of the round hole 22 in the box, the top opening edge of the sterile box 2 is in threaded connection with the edge of a cover 24, a touch screen 25 is arranged on the left side of the upper surface of the cover 24 in a slotted mode, a control panel 26 is arranged on the cover in a slotted mode on the front side of the touch screen 25, a magnetic suction seat 27 is arranged on the inner side wall of the rear side of the sterile box 2, and a clamping groove 271 is formed in the magnetic suction seat 27.
Lower surface center threaded connection of lid 24 cell separating mechanism 3, cell separating mechanism 3 includes T type fixed block 31, the lower extreme of T type fixed block 31 and the one end fixed connection of elastic helix 32, the lower extreme fixed connection holding rod 33 of elastic helix 32, control button 331 is seted up respectively from top to bottom to the holding rod 33 lateral wall, the first L type straw 34 of lower extreme fixed connection of holding rod 33, the upper portion lateral wall opening fixed connection second L type straw 35 of first L type straw 34, first L type straw 34 with link up the lower part pipe wall suit fixed block 36 at position between the second L type straw 35, fixed block 36 with draw-in groove 271 cooperation will cell separating mechanism 3 adsorbs it is fixed on the magnetism suction base 27, the left side wall fixed connection high definition digtal camera 37 of fixed block 36, it is right under high definition digtal camera 37 the terminal opening part of first L type straw 34.
The outer side wall of the rear side of the sterile box 2 is fixedly provided with the drive control mechanism 4, the drive control mechanism 4 comprises a peristaltic pump 41, an inlet pipeline 411 and an outlet pipeline 412 are respectively arranged on the peristaltic pump 41, the inlet pipeline 411 and the outlet pipeline 412 respectively penetrate through the rear side wall of the sterile box 2, the inlet pipeline 411 is communicated with the free end of the second L-shaped suction pipe 35 through a hose, and a controller 42 is arranged on the right side of the peristaltic pump 41.
The working principle of the invention is as follows: when in use, firstly, the relevant starting switch on the control panel 26 is pressed to start the whole device, then the cell mixed liquid is centrifuged by a centrifuge, then the cell mixed liquid is respectively inserted into the round holes 22 at the left and right sides of the sterile box 2 by hands to separate the whole sterile box 2 from the base 1 at the lower end, then the centrifuged cell mixed liquid is respectively poured into the first mixed liquid culture dish 15 and the second mixed liquid culture dish 16 of the base 1, then the convex edge 21 at the lower end of the sterile box 2 is inserted into the base 1 to assemble the two, then an experimenter wears sterile gloves with two hands to be respectively inserted into the round holes 22 at the left and right sides of the sterile box 2, the cell separation mechanism 3 is taken down from the magnetic suction seat 27 on the inner side wall at the rear side of the sterile box 2 by holding the holding rod 33 of the cell separation mechanism 3, then the position of the opening at the lower end of the first L-shaped suction pipe 34 is adjusted by hands, so that the single cell is searched in the first mixed liquid culture dish 15 or the second mixed liquid culture dish 16, during the process, the other hand can be released to control the relevant buttons on the control panel 26, the parameters such as the focal length, the amplification proportion and the exposure of the high-definition camera 37 are adjusted, the high-definition camera 37 amplifies the mixed liquid in the first mixed liquid culture dish 15 or the second mixed liquid culture dish 16 and transmits the real-time image to the touch screen 25 of the cover 24, the experimenter can flexibly adjust the position of the lower end opening of the first L-shaped suction pipe 34 according to the amplified image of the touch screen 25 until the single cell is searched, then the control button 331 on the holding rod 33 is pressed by fingers, at the moment, the peristaltic pump 41 is started, the generated negative pressure sucks the single cell into the first L-shaped suction pipe 34 from the lower end opening of the first L-shaped suction pipe, once the single cell is observed to be sucked into the first L-shaped suction pipe 34, the hand holding the holding rod 33 immediately inclines the bottom of the first L-shaped pipette 34 upwards while releasing the control button 331 on the holding rod 33, so that the single cell is retained in the pipette at the port of the first L-shaped pipette 34, then the first L-shaped pipette 34 is translated into the first separation liquid culture dish 17 or the second separation liquid culture dish 18, the other control button 331 on the holding rod 33 is pressed, and at this time, the peristaltic pump 41 reversely rotates to blow out the single cell in the pipette at the port of the first L-shaped pipette 34.
Electrical connections or structures not described in detail herein are prior art.
Although the present invention has been described in detail with reference to the specific embodiments, the present invention is not limited to the above embodiments, and various changes and modifications without inventive changes may be made within the knowledge of those skilled in the art without departing from the spirit of the present invention.
Claims (9)
1. A single-cell separation device, comprising: comprises a base (1), a sterile box (2), a cell separation mechanism (3) and a drive control mechanism (4); base (1) includes tray (11), tray (11) adopts transparent material, set up cross wall (12) in tray (11), callus on the sole (13) are installed respectively to four angles of tray (11) lower surface, fluorescent tube (14) are installed respectively to both sides around tray (11) lower surface, base (1) upper portion sets up aseptic box (2), chimb (21) are seted up downwards to the inside wall of aseptic box (2) lower extreme, round hole (22) are seted up at the left and right sides wall center of aseptic box (2), control the inward flange of round hole (22) is respectively to set up isolation sleeve (23) in the case, the top opening edge of aseptic box (2) and the marginal threaded connection of lid (24), the lower surface center threaded connection of lid (24) cell separation mechanism (3), cell separation mechanism (3) includes T type fixed block (31), the lower extreme and the one end fixed connection of elasticity (32) of T type fixed block (31), the lower extreme fixed connection holding rod (33) of elasticity (32), the last lower extreme fixed connection holding rod (33) of elasticity (33) is set up the L type fixed button (331) respectively straw (34) the straw upper portion is connected with the L type helix (34), straw (34) of second type spiral line (34) upper portion fixed connection of straw (34), first L type straw (34) with link up lower part pipe wall suit fixed block (36) at position between second L type straw (35), left side wall fixed connection high definition digtal camera (37) of fixed block (36), it is right under high definition digtal camera (37) the end opening part of first L type straw (34), the rear side outside wall fixed mounting of aseptic box (2) drive control mechanism (4).
2. A single-cell separation device according to claim 1, wherein: set up cross and cut off (12) and will the internal partitioning of tray (11) is first mixed liquid culture dish (15), second mixed liquid culture dish (16), first parting liquid culture dish (17) and second parting liquid culture dish (18).
3. A single-cell separation apparatus as claimed in claim 2, wherein: the upper parts of the lamp tubes (14) on the front side are dead against the left and right central lines of the first separating liquid culture dish (17) and the second separating liquid culture dish (18), and the upper parts of the lamp tubes (14) on the rear side are dead against the left and right central lines of the first mixed liquid culture dish (15) and the second mixed liquid culture dish (16).
4. A single-cell separation device according to claim 1, wherein: the sterile box (2) is made of transparent materials.
5. A single-cell separation device according to claim 1, wherein: a touch screen (25) is installed on the left side of the upper surface of the cover (24) in a groove mode, and a control panel (26) is installed on the front side of the touch screen (25) in the groove mode.
6. A single-cell separation device according to claim 1, wherein: the inner side wall of the rear side of the sterile box (2) is provided with a magnetic suction seat (27), and the magnetic suction seat (27) is provided with a clamping groove (271).
7. A single-cell separation device according to claim 6, wherein: the magnetic suction seat (27) is matched with the fixed block (36) in a magnetic suction mode through the clamping groove (271), and the cell separation mechanism (3) is adsorbed on the magnetic suction seat (27) to be fixed.
8. A single-cell separation apparatus as claimed in claim 1, wherein: the drive control mechanism (4) comprises a peristaltic pump (41), an inlet pipeline (411) and an outlet pipeline (412) are respectively arranged on the peristaltic pump (41), the inlet pipeline (411) and the outlet pipeline (412) respectively penetrate through the rear side wall of the sterile box (2), and a controller (42) is arranged on the right side of the peristaltic pump (41).
9. A single-cell separation apparatus according to claim 8 wherein: the inlet pipeline (411) is communicated with the free end of the second L-shaped suction pipe (35) through a hose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210728649.XA CN115181634A (en) | 2022-06-24 | 2022-06-24 | Single cell separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210728649.XA CN115181634A (en) | 2022-06-24 | 2022-06-24 | Single cell separation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115181634A true CN115181634A (en) | 2022-10-14 |
Family
ID=83515024
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210728649.XA Withdrawn CN115181634A (en) | 2022-06-24 | 2022-06-24 | Single cell separation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115181634A (en) |
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2022
- 2022-06-24 CN CN202210728649.XA patent/CN115181634A/en not_active Withdrawn
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Application publication date: 20221014 |