CN115137837B - Application of polyvinylpyrrolidone modified palladium nanoparticles - Google Patents
Application of polyvinylpyrrolidone modified palladium nanoparticles Download PDFInfo
- Publication number
- CN115137837B CN115137837B CN202211075886.7A CN202211075886A CN115137837B CN 115137837 B CN115137837 B CN 115137837B CN 202211075886 A CN202211075886 A CN 202211075886A CN 115137837 B CN115137837 B CN 115137837B
- Authority
- CN
- China
- Prior art keywords
- polyvinylpyrrolidone
- nps
- pvp
- fudr
- nematodes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000002105 nanoparticle Substances 0.000 title claims abstract description 63
- 239000001267 polyvinylpyrrolidone Substances 0.000 title claims abstract description 43
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 title claims abstract description 43
- 229920000036 polyvinylpyrrolidone Polymers 0.000 title claims abstract description 43
- 150000002940 palladium Chemical class 0.000 title claims description 32
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 claims abstract description 185
- 230000002438 mitochondrial effect Effects 0.000 claims abstract description 36
- 229910052763 palladium Inorganic materials 0.000 claims abstract description 34
- 102000004190 Enzymes Human genes 0.000 claims abstract description 33
- 108090000790 Enzymes Proteins 0.000 claims abstract description 33
- 230000002708 enhancing effect Effects 0.000 claims abstract description 23
- 230000032683 aging Effects 0.000 claims abstract description 22
- 230000002503 metabolic effect Effects 0.000 claims abstract description 19
- 230000008642 heat stress Effects 0.000 claims abstract description 14
- 230000006540 mitochondrial respiration Effects 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims description 16
- 230000004202 respiratory function Effects 0.000 claims description 12
- 230000003712 anti-aging effect Effects 0.000 claims description 10
- 235000013373 food additive Nutrition 0.000 claims description 9
- 239000002778 food additive Substances 0.000 claims description 9
- 239000002245 particle Substances 0.000 claims description 9
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 8
- 230000035882 stress Effects 0.000 claims description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims description 5
- 239000002417 nutraceutical Substances 0.000 claims description 5
- 235000021436 nutraceutical agent Nutrition 0.000 claims description 5
- 210000003470 mitochondria Anatomy 0.000 abstract description 19
- 230000000694 effects Effects 0.000 abstract description 16
- 238000002360 preparation method Methods 0.000 abstract description 16
- 230000009467 reduction Effects 0.000 abstract description 4
- 241000244206 Nematoda Species 0.000 description 120
- ODKNJVUHOIMIIZ-RRKCRQDMSA-N floxuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(F)=C1 ODKNJVUHOIMIIZ-RRKCRQDMSA-N 0.000 description 72
- 239000000047 product Substances 0.000 description 58
- 241000244203 Caenorhabditis elegans Species 0.000 description 37
- 239000000243 solution Substances 0.000 description 34
- 238000002156 mixing Methods 0.000 description 23
- 241001167795 Escherichia coli OP50 Species 0.000 description 19
- 230000001580 bacterial effect Effects 0.000 description 17
- 239000011248 coating agent Substances 0.000 description 16
- 238000000576 coating method Methods 0.000 description 16
- 102100030497 Cytochrome c Human genes 0.000 description 14
- 108010075031 Cytochromes c Proteins 0.000 description 14
- 239000000203 mixture Substances 0.000 description 14
- 239000003814 drug Substances 0.000 description 13
- 230000036284 oxygen consumption Effects 0.000 description 13
- 239000007788 liquid Substances 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 10
- 238000012258 culturing Methods 0.000 description 9
- 230000002401 inhibitory effect Effects 0.000 description 9
- 230000017448 oviposition Effects 0.000 description 9
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 8
- 238000010521 absorption reaction Methods 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 8
- 230000004083 survival effect Effects 0.000 description 8
- 102000000634 Cytochrome c oxidase subunit IV Human genes 0.000 description 7
- 108090000365 Cytochrome-c oxidases Proteins 0.000 description 7
- 238000005452 bending Methods 0.000 description 7
- 230000001965 increasing effect Effects 0.000 description 7
- 230000029058 respiratory gaseous exchange Effects 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 239000002552 dosage form Substances 0.000 description 6
- 229940079593 drug Drugs 0.000 description 6
- 230000036541 health Effects 0.000 description 6
- 230000002829 reductive effect Effects 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- ABKQFSYGIHQQLS-UHFFFAOYSA-J sodium tetrachloropalladate Chemical compound [Na+].[Na+].Cl[Pd+2](Cl)(Cl)Cl ABKQFSYGIHQQLS-UHFFFAOYSA-J 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 5
- 241001559542 Hippocampus hippocampus Species 0.000 description 5
- 101150003085 Pdcl gene Proteins 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 5
- 239000007853 buffer solution Substances 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 230000007423 decrease Effects 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 230000000968 intestinal effect Effects 0.000 description 5
- 230000033001 locomotion Effects 0.000 description 5
- 230000004898 mitochondrial function Effects 0.000 description 5
- 230000034958 pharyngeal pumping Effects 0.000 description 5
- 238000003753 real-time PCR Methods 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 238000009825 accumulation Methods 0.000 description 4
- 230000001154 acute effect Effects 0.000 description 4
- 229960005070 ascorbic acid Drugs 0.000 description 4
- 235000010323 ascorbic acid Nutrition 0.000 description 4
- 239000011668 ascorbic acid Substances 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- PIBWKRNGBLPSSY-UHFFFAOYSA-L palladium(II) chloride Chemical compound Cl[Pd]Cl PIBWKRNGBLPSSY-UHFFFAOYSA-L 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 230000035806 respiratory chain Effects 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 108010030844 2-methylcitrate synthase Proteins 0.000 description 3
- 230000002407 ATP formation Effects 0.000 description 3
- 108010071536 Citrate (Si)-synthase Proteins 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000006399 behavior Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 239000002775 capsule Substances 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 210000003800 pharynx Anatomy 0.000 description 3
- 239000006187 pill Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 230000027756 respiratory electron transport chain Effects 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000003826 tablet Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 101100246536 Caenorhabditis elegans pyc-1 gene Proteins 0.000 description 2
- BMZRVOVNUMQTIN-UHFFFAOYSA-N Carbonyl Cyanide para-Trifluoromethoxyphenylhydrazone Chemical compound FC(F)(F)OC1=CC=C(NN=C(C#N)C#N)C=C1 BMZRVOVNUMQTIN-UHFFFAOYSA-N 0.000 description 2
- 102000006732 Citrate synthase Human genes 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 2
- 239000012190 activator Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000012295 chemical reaction liquid Substances 0.000 description 2
- 239000007810 chemical reaction solvent Substances 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 230000003412 degenerative effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002073 fluorescence micrograph Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000036571 hydration Effects 0.000 description 2
- 238000006703 hydration reaction Methods 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000006705 mitochondrial oxidative phosphorylation Effects 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 230000004899 motility Effects 0.000 description 2
- 208000015122 neurodegenerative disease Diseases 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000009747 swallowing Effects 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000007492 two-way ANOVA Methods 0.000 description 2
- 238000002371 ultraviolet--visible spectrum Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 108091006112 ATPases Proteins 0.000 description 1
- 102000057290 Adenosine Triphosphatases Human genes 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 101150110592 CTS1 gene Proteins 0.000 description 1
- 101001059929 Caenorhabditis elegans Forkhead box protein O Proteins 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 235000009161 Espostoa lanata Nutrition 0.000 description 1
- 240000001624 Espostoa lanata Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000819999 Nymphes Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 108010001267 Protein Subunits Proteins 0.000 description 1
- 102000002067 Protein Subunits Human genes 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 102000011990 Sirtuin Human genes 0.000 description 1
- 108050002485 Sirtuin Proteins 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000004103 aerobic respiration Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000010226 confocal imaging Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 238000012407 engineering method Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000004153 glucose metabolism Effects 0.000 description 1
- 230000006545 glycolytic metabolism Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- -1 hxk-1 Proteins 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000001325 log-rank test Methods 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 210000003712 lysosome Anatomy 0.000 description 1
- 230000001868 lysosomic effect Effects 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 230000005787 mitochondrial ATP synthesis coupled electron transport Effects 0.000 description 1
- 210000001700 mitochondrial membrane Anatomy 0.000 description 1
- 230000006677 mitochondrial metabolism Effects 0.000 description 1
- 230000008811 mitochondrial respiratory chain Effects 0.000 description 1
- 239000010413 mother solution Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 230000004770 neurodegeneration Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 230000010627 oxidative phosphorylation Effects 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000008844 regulatory mechanism Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 1
- 239000004061 uncoupling agent Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
- A61K47/58—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nanotechnology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Epidemiology (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Inorganic Chemistry (AREA)
- Manufacturing & Machinery (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- Materials Engineering (AREA)
- Composite Materials (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to application of palladium nanoparticles, and discloses application of palladium nanoparticles modified by polyvinylpyrrolidone, which comprises application of palladium nanoparticles modified by polyvinylpyrrolidone in preparation of a product for resisting aging and/or enhancing heat stress resistance, application of palladium nanoparticles modified by polyvinylpyrrolidone in preparation of a product for improving mitochondrial respiration function, and application of palladium nanoparticles modified by polyvinylpyrrolidone in a product for enhancing expression of mitochondrial metabolic enzyme. The palladium nano-particles modified by the polyvinylpyrrolidone can reverse the function reduction of mitochondria caused by aging so as to achieve the effects of delaying aging and resisting aging.
Description
Technical Field
The invention relates to application of palladium nanoparticles, in particular to application of palladium nanoparticles modified by polyvinylpyrrolidone in preparing products for resisting aging and/or enhancing heat stress resistance, application of palladium nanoparticles modified by polyvinylpyrrolidone in preparing products for improving mitochondrial respiration function, and application of palladium nanoparticles modified by polyvinylpyrrolidone in preparing products for enhancing expression of mitochondrial metabolic enzymes.
Background
The health and longevity are the targets of people unchanged from the ancient times. Nowadays, with the continuous improvement of the average life span of human beings, the aging problem is increasingly highlighted. Aging has become a medical problem and a social problem which are becoming more and more serious, and is considered to be a major factor in the onset of neurodegenerative diseases, tumors, cardiovascular diseases and diabetes, which are characterized by degenerative phenomena occurring at the molecular, cellular organelle, tissue and even whole organism level. The research and development of anti-aging drugs and the clarification of the regulation mechanism thereof have important practical significance for relieving the increasingly serious aging phenomenon and the pressure of the sustainable development of economy and society.
Cytochrome c oxidase (mitochondrial complex IV) is an enzyme complex of respiratory chain enzyme, is a key enzyme in respiratory chain enzyme, participates in oxidative phosphorylation and many reactions in tricarboxylic acid cycle, is an important cofactor for mitochondrial function exertion and biosynthesis, and the change of enzyme activity directly affects the function change of the whole respiratory chain. However, cytochrome c oxidase is unstable and fragile, and is vulnerable to free radical oxidation, and the destruction of its protein subunit can cause electron loss during electron transfer, thereby reducing the electron transfer efficiency, further affecting the function of respiratory chain and insufficient energy supply of cells, and reducing the metabolic capability of the body, resulting in aging and aging-related diseases. Researches find that in tissues rich in mitochondria such as heart, brain and the like, various diseases related to aging are related to the structural and functional disorder of cytochrome c oxidase; similarly, mitochondrial enzyme activity is also reduced within neurodegenerative disorders.
Previous researches show that the mitochondrial respiratory chain enzyme is reconstructed, expressed and purified by an enzyme engineering method, but the natural enzyme has the problems of insufficient stability, complicated reconstruction and purification, high preparation cost and the like. Therefore, the research on the anti-aging nano enzyme with high physical and chemical stability, simple preparation process and low cost has great significance.
Disclosure of Invention
The invention aims to solve the problems of poor stability, complex synthesis process and high cost of natural enzymes in the prior art, and provides the application of the palladium nanoparticle modified by polyvinylpyrrolidone.
In order to achieve the above objects, the present invention provides, in a first aspect, the use of polyvinylpyrrolidone-modified palladium nanoparticles for the preparation of a product for anti-aging and/or enhancing the ability to resist heat stress.
Preferably, the particle size of the polyvinylpyrrolidone-modified palladium nanoparticle is 15-25nm.
Preferably, in the polyvinylpyrrolidone-modified palladium nanoparticle, the molar ratio of polyvinylpyrrolidone to palladium is 1-9.
Preferably, the anti-aging and/or anti-heat stress enhancing product is selected from at least one of a drug, a health product and a food additive.
In a second aspect, the invention provides the use of polyvinylpyrrolidone-modified palladium nanoparticles in the preparation of a product for improving mitochondrial respiratory function.
Preferably, the particle size of the polyvinylpyrrolidone-modified palladium nanoparticle is 15-25nm.
Preferably, in the polyvinylpyrrolidone-modified palladium nanoparticle, the molar ratio of polyvinylpyrrolidone to palladium is 1-9.
Preferably, the mitochondrial respiration function improving product is selected from at least one of a drug, a nutraceutical, and a food additive.
In a third aspect, the invention provides the use of polyvinylpyrrolidone-modified palladium nanoparticles in the preparation of a product for enhancing expression of a mitochondrial metabolic enzyme.
Preferably, the particle size of the polyvinylpyrrolidone-modified palladium nanoparticle is 15-25nm.
Preferably, in the polyvinylpyrrolidone-modified palladium nanoparticle, the molar ratio of polyvinylpyrrolidone to palladium is 1-9.
Preferably, the product for enhancing the expression of mitochondrial metabolic enzyme is selected from at least one of a drug, a health product and a food additive.
Preferably, the metabolic enzyme is selected from citrate synthasects-1Hexokinasehxk-1And pyruvate carboxylasepyc-1At least one of (1).
Through the technical scheme, the invention has the beneficial effects that:
the invention provides a new function and a new application of palladium nanoparticles modified by polyvinylpyrrolidone, and the inventor discovers for the first time that the palladium nanoparticles modified by polyvinylpyrrolidone can increase the basic respiration rate, the maximum respiration rate and the ATP generation of organism mitochondria so as to enhance the respiration function of the organism mitochondria, and can be effectively applied to the preparation of products for improving the respiration function of the mitochondria; the palladium nanoparticles modified by polyvinylpyrrolidone can prolong the life of organisms and make them oldThe pharyngeal exercise (eating) ability and the head swing ability of the old organism are increased, the lipofuscin accumulation amount is reduced, and the method can be effectively applied to the preparation of the product for resisting aging and/or enhancing the heat stress resistance; in addition, the palladium nano-particles modified by polyvinylpyrrolidone also have the function of enhancing metabolic enzyme citrate synthase in organism mitochondriacts-1Hexokinasehxk-1And pyruvate carboxylasepyc-1The efficacy of (2) can be effectively applied to the preparation of products for enhancing the expression of mitochondrial metabolic enzymes.
Additional features and advantages of the invention will be set forth in the detailed description which follows.
Drawings
FIG. 1 is a graph of UV-VIS absorption spectra of PVP-Pd NPs at various concentrations in example 1 after interaction with cytochrome c;
FIG. 2 is a graph of UV-VIS absorption spectra of PVP-Pd NPs in example 1 after exposure to cytochrome c for various periods of time;
FIG. 3 is a graph showing the basal oxygen consumption and maximum oxygen consumption of nematodes in the PVP-Pd NPs-treated group and the control group in example 2, wherein A is a graph showing the basal oxygen consumption of nematodes in the PVP-Pd NPs-treated group and the control group, and B is a graph showing the maximum oxygen consumption of nematodes in the PVP-Pd NPs-treated group and the control group;
FIG. 4 is a graph of ATP levels in nematodes of the PVP-Pd NPs treated group and the control group of example 3;
FIG. 5 is a graph showing mRNA levels of various metabolic enzymes in mitochondria of nematodes in the PVP-Pd NPs-treated group and the control group in example 4;
FIG. 6 is a graph of mean nematode life time for the PVP-Pd NPs treated group and the control group of example 5;
FIG. 7 is a graph of nematode survival for the PVP-Pd NPs treated group and the control group of example 5;
FIG. 8 is fluorescence images of the nematode lipofuscin of the PVP-Pd NPs treated group and the control group of example 6;
FIG. 9 is a graph of quantitative data on nematode lipofuscin in the PVP-Pd NPs treated group and the control group of example 6;
FIG. 10 is a graph of the number of head swings of nematodes in the group to which PVP-Pd NPs were administered and the control group in example 7;
FIG. 11 is a graph of nematode pharyngeal pumping frequency for the PVP-Pd NPs administration group and the control group of example 8;
FIG. 12 is a graph showing the survival rate of nematodes under heat stress and the number of times of body bending in the PVP-Pd NPs-administered group and the control group in example 9.
Detailed Description
The endpoints of the ranges and any values disclosed herein are not limited to the precise range or value, and such ranges or values should be understood to encompass values close to those ranges or values. For ranges of values, between the endpoints of each of the ranges and the individual points, and between the individual points may be combined with each other to give one or more new ranges of values, and these ranges of values should be considered as specifically disclosed herein.
The invention provides application of palladium nanoparticles modified by polyvinylpyrrolidone in preparing products for resisting aging and/or enhancing heat stress resistance.
In the research process, by observing the influences of the life, aging-related physiology and behavior indexes of organisms (such as nematodes and human bodies) treated by the palladium nanoparticles modified by polyvinylpyrrolidone, the inventor of the invention proves that the palladium nanoparticles modified by polyvinylpyrrolidone can prolong the life of the organisms, increase the pharyngeal exercise (eating) ability and the body bending ability of the aged organisms, reduce the lipofuscin accumulation amount, and have the effects of delaying aging, resisting aging and reversing the function reduction caused by aging.
In the present invention, the polyvinylpyrrolidone-modified palladium nanoparticles may be commercially available or may be prepared by a method disclosed in the prior art.
Illustratively, the method for preparing polyvinylpyrrolidone-modified palladium nanoparticles comprises the following steps: in a reaction solvent I, sodium tetrachloropalladate (Na) 2 PdCl 4 ) Mixing the palladium nanoparticle with polyvinylpyrrolidone (PVP), heating to 50-70 ℃, mixing the PVP with ascorbic acid for reaction to obtain a reaction solution, and separating and purifying the reaction solution to obtain the polyvinylpyrrolidone modified palladium nanoparticle.
In the present invention, sodium tetrachloropalladate is commercially available, or can be obtained by mixing palladium chloride with sodium chloride.
According to the invention, the sodium tetrachloropalladate and the polyvinylpyrrolidone can be mixed in any proportion. In order to further improve the effect of polyvinylpyrrolidone on palladium nanoparticle modification and better control the particle size of polyvinylpyrrolidone-modified palladium nanoparticle, the molar ratio of sodium tetrachloropalladate to polyvinylpyrrolidone is preferably 1 to 10-15.
In the invention, the reaction solvent I can adopt deionized water, and specifically, the process of mixing the sodium tetrachloropalladate and the polyvinylpyrrolidone is as follows: preparing the sodium tetrachloropalladate into a solution with the concentration of 0.04-0.06mol/L by using deionized water, preparing the polyvinylpyrrolidone into a solution with the concentration of 0.5-0.7mol/L by using the deionized water, and then mixing in the form of aqueous solution.
According to the invention, the mixing with ascorbic acid is carried out by: adding an aqueous solution of ascorbic acid in a dropwise manner; the reaction conditions include: the temperature is 50-70 deg.C, and the time is 50-70min.
In the present invention, the separation and purification processes may adopt solid-liquid separation and purification methods conventional in the art, for example, solid-liquid separation may be performed by filtration and centrifugation, and purification may be performed by washing, membrane filtration, and the like.
In the invention, specifically, the preparation process of the palladium nanoparticle modified by polyvinylpyrrolidone comprises the following steps: mixing palladium chloride and sodium chloride aqueous solution to prepare Na with the concentration of 0.05mol/L 2 PdCl 4 Solution, 10mL Na 2 PdCl 4 The solution was mixed with 10mL of deionized water, and 10mL of a 0.6mol/L aqueous solution of polyvinylpyrrolidone was added to Na with magnetic stirring 2 PdCl 4 In the solution, the temperature is raised and the water bath is kept at 60 ℃, and then10mL of ascorbic acid aqueous solution with the concentration of 0.06mol/L is dripped into the solution within 15min, the solution is continuously stirred for 60min at the temperature after the dripping is finished, reaction liquid is obtained, the reaction liquid is subjected to solid-liquid separation to obtain separation and precipitation, and the separation and precipitation is purified to obtain the palladium nano-particles modified by polyvinylpyrrolidone (hereinafter referred to as PVP-Pd NPs).
According to the present invention, the particle size of the polyvinylpyrrolidone-modified palladium nanoparticle is preferably 15 to 25nm, and specifically may be 15 nm, 17 nm, 19 nm, 21 nm, 23 nm, 25nm, or any value in a range of any two of the above values. The inventor finds that under the preferred embodiment, the absorption of the nano-particles by organisms is favorably improved, and the aging delaying effect and the heat stress resistance of the nano-particles are further improved.
According to the present invention, preferably, in the polyvinylpyrrolidone-modified palladium nanoparticle, the molar ratio of polyvinylpyrrolidone to palladium is 1 to 9.
In the invention, the anti-aging product can be a product for prolonging the life of an organism, can also be a product for improving the motion ability of the organism in the aspects of pharyngeal motion ability, body bending ability, head swinging ability and the like, or a product for reducing the lipofuscin accumulation amount in the organism; the product for enhancing the heat stress resistance can be a product for enhancing the resistance of an organism to external heat stress (namely the heat stress resistance), and the external heat stress of the organism refers to a phenomenon that the highest point of a temperature range for growth adaptation of the organism generates metabolism and growth and development stress to the organism, for example, the external heat stress temperature of nematodes can be 32-40 ℃.
According to the present invention, preferably, the anti-aging and/or anti-heat stress enhancing product is at least one selected from the group consisting of a drug, a health product and a food additive. The dosage form of the medicine or the health-care product is oral liquid, capsules, tablets, powder, granules or pills, and the medicine or the health-care product of various dosage forms can be prepared according to the conventional method in the field and can also comprise a carrier acceptable in pharmacy or health-care products. The content of the polyvinylpyrrolidone-modified palladium nanoparticles in the anti-aging and/or anti-heat stress enhancing product can be designed and adjusted according to dosage.
In a second aspect, the invention provides the use of polyvinylpyrrolidone-modified palladium nanoparticles in the preparation of a product for improving mitochondrial respiratory function.
The inventor of the invention further discovers, through research and observation, that the palladium nanoparticles modified by polyvinylpyrrolidone can increase the basal respiration rate, the maximum respiration rate and the ATP production of organism mitochondria, further enhance the respiratory function of organism mitochondria, and can be effectively applied to the preparation of products for promoting the mitochondrial respiration.
The polyvinylpyrrolidone-modified palladium nanoparticles according to the present invention may be commercially available or may be prepared by methods disclosed in the prior art. Preferably, the particle size of the polyvinylpyrrolidone-modified palladium nanoparticle is 15-25nm. The inventors found that, in the preferred embodiment, it is advantageous to increase the nanoparticle absorption by the living body, and thereby to improve the effect of the nanoparticle on improving the mitochondrial respiratory function of the living body.
According to the present invention, preferably, in the polyvinylpyrrolidone-modified palladium nanoparticle, the molar ratio of polyvinylpyrrolidone to palladium is 1 to 9.
In the present invention, the product for improving mitochondrial respiratory function may be a product for regulating mitochondrial electron transport chain disorder, a product for regulating mitochondrial metabolic enzyme disorder, or a product for increasing cellular ATP synthesis.
According to the present invention, preferably, the mitochondrial respiration function improving product is selected from at least one of a drug, a health product and a food additive. The dosage form of the medicine or the health-care product is oral liquid, capsules, tablets, powder, granules or pills, and the medicine or the health-care product of various dosage forms can be prepared according to the conventional method in the field and can also comprise a carrier acceptable in pharmacy or health-care products. The content of the polyvinylpyrrolidone-modified palladium nanoparticles in the product for improving the mitochondrial respiratory function can be designed and adjusted according to the dosage.
In a third aspect, the invention provides the use of polyvinylpyrrolidone-modified palladium nanoparticles in the preparation of a product for enhancing expression of a mitochondrial metabolic enzyme.
The inventors of the present invention have also surprisingly found that polyvinylpyrrolidone-modified palladium nanoparticles have the ability to enhance citrate synthase in mitochondria of organismscts-1Hexokinasehxk-1And pyruvate carboxylasepyc-1The efficacy of (2) can be effectively applied to the preparation of products for enhancing the expression of mitochondrial metabolic enzymes.
According to the present invention, the polyvinylpyrrolidone-modified palladium nanoparticles may be commercially available or may be prepared by a method disclosed in the prior art. Preferably, the particle size of the polyvinylpyrrolidone-modified palladium nanoparticle is 15-25nm. The inventors have found that in this preferred embodiment, it is advantageous to increase the uptake of the nanoparticles by the organism, and thus to increase the enhancement of mitochondrial metabolic enzymes in the organism.
According to the present invention, preferably, in the polyvinylpyrrolidone-modified palladium nanoparticle, the molar ratio of polyvinylpyrrolidone to palladium is 1 to 9.
According to the present invention, preferably, the product for enhancing the expression of mitochondrial metabolism enzyme is selected from at least one of pharmaceuticals, nutraceuticals, and food additives. The dosage form of the medicine or the health-care product is oral liquid, capsules, tablets, powder, granules or pills, and the medicine or the health-care product of various dosage forms can be prepared according to the conventional method in the field and can also comprise a carrier acceptable in pharmacy or health-care products. The content of polyvinylpyrrolidone-modified palladium nanoparticles in the product for enhancing the expression of mitochondrial metabolic enzymes can be designed and adjusted according to dosage.
According to the invention, the metabolic enzyme may be any enzyme of an organism involved in mitochondrial metabolic processes. Preferably, the metabolic enzyme is selected from citrate synthasects-1Hexokinasehxk-1And pyruvate carboxylasepyc-1At least one of (1).
The present invention will be described in detail below by way of examples.
In the following examples and comparative examples, polyvinylpyrrolidone modified palladium nanoparticles (PVP-Pd NPs) were obtained from qiangsu jufeng nanomaterial science co ltd, product No. XFJ117, particle size 15-25nm, molar ratio of polyvinylpyrrolidone to palladium 5, cytochrome c was obtained from yuye (shanghai) co ltd, product No. 9007-43-6, mitochondrial oxidative phosphorylation uncoupling agent FCCP was obtained from seleck co ltd, product No. S8276, palladium chloride PdCl 8276 2 From Allantin reagent (Shanghai) Inc., with product number 7647-10-1, FUDR for inhibiting nematode oviposition, from Allantin reagent (Shanghai) Inc., with product number 50-91-9; the mitochondrial ATP detection kit is purchased from Saimer Feishell science and technology (China) GmbH, and has a product number of A22066;cts-1, hxk-1, pyc-1 andreference geneOf actinThe q-PCR primer is prepared by Shanghai Biotechnology Limited, and the specific primer is as follows: the NAD + /Sirtuin Pathway Modulates Longevity through Activation of Mitochondrial UPR and FOXO Signaling，Laurent Mouchiroud，et alCell, volume 154, issue 2, 18 July 2013, pages 430-441; the q-PCR reverse transcription kit is purchased from Promega reagent company Limited, and the product number is A5001; taq Pro Universal SYBR aPCR Master mix available from Promega reagent Inc., product number Q712-02; the mitochondrial ATP detection kit is purchased from Biyunnan reagent Limited, and the product number is S0026B; the rest chemical reagents and raw materials are conventional commercial products.
In the following examples and comparative examples, a confocal laser microscope was purchased from Nikon, japan, a luminescence detector was purchased from Promega, a mitochondrial function measurement System Seahorse XF24 was purchased from Seahorse biosciences, USA, and an ultraclean bench was purchased from Suzhou gold Equipment science and technology, inc.
In the following examples and comparative examples, wild-type N2 C.elegans was obtained from the genetic center of C.elegans (university of Minnesota, USA); escherichia coli OP50 is purchased from NTCC type culture collection center and is numbered as NTCC445651, and the culture process of the bacterial liquid is as follows: escherichia coli OP50 was inoculated into LB medium and cultured in a shaking incubator at a rotation speed of 220rpm and a temperature of 37 ℃.
In the following examples and comparative examples, the procedure for configuring NGM slabs was: 3g of NaCl, 17g of agar and 2.5g of peptone, adding double distilled water to 1000mL, adjusting the pH value to 6.0, and sterilizing to obtain a basic culture medium; the water bath at 55 ℃ is balanced for 15min, so that the solidification of a basic culture medium caused by too low temperature is prevented. In a super clean bench, after ultraviolet sterilization of a basic culture dish, a magnetic stirrer, gloves and substances to be added, firstly, a conical flask filled with a basic culture medium is placed on the magnetic stirrer for stirring, the rotation speed is based on no generation of bubbles, then, alcohol cotton balls are used for wiping the gloves clean, a culture plate package and a bottleneck sealing film (the gloves do not touch the bottleneck) are disassembled, and 25mL KH is sequentially added into the basic culture medium 2 PO 4 Solution (mother liquor concentration: 1M), 1mL CaCl 2 Solution (mother liquor concentration: 1M), 1mL MgSO 4 Slowly dripping 1mL of cholesterol solution (mother solution concentration: 5 mg/mL) along the bottle wall during stirring to fully and uniformly mix the cholesterol solution with the solution in the bottle until no visible oil drop exists on the surface of the solution to obtain an NGM culture medium; pouring the NGM culture medium on a culture dish with the diameter of 9cm, making the NGM culture medium have no visible air bubbles as much as possible, standing the culture dish at room temperature for one day after plate pouring, and solidifying the culture medium for later use.
In the following examples and comparative examples, the synchronization process for caenorhabditis elegans was:
collecting caenorhabditis elegans adults in a good growth state into a centrifuge tube, and adding 10mL M9 buffer solution to wash away redundant escherichia coli OP50; and (3) reserving 4mL of supernatant in a centrifugal tube, adding 500 mu L of 5M NaOH solution and 500 mu L of 5% sodium hypochlorite solution, oscillating for about 3min on a vortex oscillator, detecting under an electron microscope while oscillating, cleaning 3 times by using M9 buffer solution as soon as possible when a nymph body is broken, centrifuging by using a miniature centrifuge after cleaning, removing redundant solution and leaving ova, adding 500 mu L of ova to a blank NGM culture plate, and culturing for 12-16h in an incubator at 20 ℃ so that the ova are incubated into synchronized L1 larvae.
Example 1
To test the cytochrome c oxidase mimetic activity of PVP-Pd NPs, 60. Mu.L of a 10mg/mL cytochrome c solution (using PBS buffer as a solvent) and 400. Mu.L of different concentrations of PVP-Pd NPs (0.1. Mu.g/mL, 0.2. Mu.g/mL, 1. Mu.g/mL, 10. Mu.g/mL, 50. Mu.g/mL, 100. Mu.g/mL of PVP-Pd NPs) were added in sequence to a 5mL EP tube containing 4mL of a PBS buffer solution (phosphate concentration of 10mM, pH 7.4), and UV-visible absorption measurement was performed after 60min of reaction, and PVP-Pd NPs concentration of 0 was used as a control (control), as shown in FIG. 1.
The results are shown in FIG. 2, in which 60. Mu.L of a 10mg/mL cytochrome c solution (using PBS buffer as a solvent) and 400. Mu.L of a 1. Mu.g/mL PVP-Pd NPs solution were added in this order to a 5mL EP tube containing 4mL of a PBS buffer solution (concentration: 10mM, pH 7.4) and reacted for 2min,7min, 111min, 15min,19min,22min,26min,30min, followed by UV-visible absorption measurement, and the reaction time was 0 as a control (control).
The results of FIGS. 1 and 2 demonstrate cytochrome c oxidase-like activity of PVP-Pd NPs. As can be seen from FIG. 1, the control group (control) readily detected a higher absorption peak at the alpha band (550 nm) of iron cytochrome c, indicating the presence of cytochrome c in the form of ferrous iron. In contrast, after the PVP-Pd NPs with the concentration of between 0.1 and 100 mu g/mL are treated, the absorption peak of cytochrome c in a ferrous form is reduced, and the concentration of the PVP-Pd NPs is dependent, and the fact that the PVP-Pd NPs with the concentration of between 0.1 and 100 mu g/mL can convert cytochrome c in the ferrous form into cytochrome c in a ferric form is proved, and the cytochrome c oxidase catalysis function of the PVP-Pd NPs is proved. As can be seen from FIG. 2, after the PVP-Pd NPs are added into the reaction system, the characteristic absorption of the iron cytochrome c gradually shows time-dependent reduction within the time range of 2min to 30min, and the decrease of the absorption peak of the cytochrome c in the form of ferrous iron can be obviously detected after the reaction is carried out for 7min, thus confirming the reaction sensitivity of the catalytic function of the cytochrome c oxidase similar to the PVP-Pd NPs. The above results indicate that PVP-Pd NPs are able to mimic the activity of cytochrome c oxidase in vitro, catalyzing the oxidation of cytochrome c in the ferrous form.
Example 2
Mitochondrial function is particularly important for understanding energy metabolism of cells, and the oxygen consumption of cells can reflect mitochondrial function. Therefore, the effect of PVP-Pd NPs on improving the mitochondrial respiratory function can be researched by researching the oxygen consumption rate of mitochondria. Adding PVP-Pd NPs and FUDR into an escherichia coli OP50 bacterial solution, uniformly mixing, uniformly coating the mixture on an NGM flat plate to obtain an NGM flat plate containing PVP-Pd NPs and FUDR (the final concentration of the PVP-Pd NPs is 0.5 mu g/mL, the final concentration of the FUDR is 80 mu M, and the final concentration of the FUDR is used for inhibiting the oviposition of caenorhabditis elegans), adding FUDR into the escherichia coli OP50 bacterial solution, uniformly mixing, and uniformly coating the mixture on the NGM flat plate to obtain an NGM flat plate (the final concentration of the FUDR is 80 mu M) containing only FUDR and not containing PVP-Pd NPs; carrying out nematode synchronization treatment by adopting a wild caenorhabditis elegans model, and transferring a part of L4-stage nematode strains to an NGM flat plate containing PVP-Pd NPs and FUDR as an administration group when L1-stage wild type N2 caenorhabditis elegans grows to L4 stage; transferring another part of L4-stage nematode strains to NGM plates which do not contain PVP-Pd NPs and only contain FUDR to serve as a control group, culturing about 50-100 nematodes in each group at 20 ℃, collecting the nematodes at 7 days of adult insects, and measuring oxygen consumption. Specifically, the oxygen consumption of nematodes was measured using the mitochondrial function assay system Seahorse XF24, specifically, the test plate was added 1mL of hydration per well one day in advance and placed in a 37 ℃ incubator overnight for hydration, and the nematodes were then placed in a 24-well standard Seahorse plate (18 nematodes per well). Using Seahorse XF-24 software, the assay template was loaded, mitochondrial oxidative phosphorylation uncoupler FCCP (final concentration: 20. Mu.M) was injected into each well to induce maximal respiration, and NaN was added 3 (final concentration: 40 mM) was used to distinguish non-mitochondrial respiration, and the maximum and basal oxygen consumption rates per nematode were calculated on average for the treatment and control groups, and the results are shown in FIG. 3.
As can be seen from FIG. 3, PVP-Pd NPs enhance mitochondrial respiration in senile nematodes; specifically, on adult day 7, the nematodes in the control group easily detected a certain basal oxygen consumption and maximum oxygen consumption. The basal oxygen consumption (shown as a in fig. 3) and the maximum oxygen consumption (shown as B in fig. 3) were significantly increased in the PVP-Pd NPs-treated group compared to the control group. The result shows that the PVP-Pd NPs can improve the respiratory function of mitochondria, so the PVP-Pd NPs can be used as an effective mitochondrial activator and have important application prospect.
Example 3
Mitochondria are the main site for aerobic respiration and energy conversion of cells, and are self-understood as the capacity factory of cells, most energy required for cell life activities comes from mitochondria, and the transfer of electron transfer on the inner membrane of mitochondria promotes proton discharge to the mitochondrial membrane gap, and finally, ADP is converted into ATP through ATP synthase for energy supply. Therefore, the effect of PVP-Pd NPs on improving the mitochondrial respiratory function can be researched by researching the ATP generation of mitochondria. Adding PVP-Pd NPs and FUDR into Escherichia coli OP50 bacterial liquid, mixing, uniformly coating on an NGM flat plate to obtain an NGM flat plate containing PVP-Pd NPs and FUDR (the final concentration of PVP-Pd NPs is 0.5 mu g/mL; the final concentration of FUDR is 80 mu M, and is used for inhibiting the oviposition of caenorhabditis elegans), adding FUDR into Escherichia coli OP50 bacterial liquid, mixing, and uniformly coating on an NGM flat plate to obtain an NGM flat plate containing only FUDR and not containing PVP-Pd NPs (the final concentration of FUDR is 80 mu M); carrying out nematode synchronization treatment by adopting a wild caenorhabditis elegans model, and transferring a part of L4-stage nematode strains to an NGM flat plate containing PVP-Pd NPs and FUDR as an administration group when L1-stage wild type N2 caenorhabditis elegans grows to the L4 stage; another part of the L4-stage nematode strains was transferred to NGM plates containing only FUDR without PVP-Pd NPs as a control group, cultured at 20 ℃ in an incubator, and the nematodes were collected at day 7 of the adult worms and the mitochondrial ATP yield was measured, specifically, about 100-200 nematodes were collected per group and resuspended in M9 buffer for 5 freeze/thaw cycles (from liquid nitrogen to 40 ℃), then boiled for 20min to release ATP and destroy ATPase activity before centrifugation, and the diluted supernatant was used for ATP measurement according to the instructions of the ATP detection kit, the results are shown in FIG. 4.
The results in FIG. 4 show that PVP-Pd NPs increase mitochondrial productivity of senile nematodes; specifically, on day 7 of adult worms, the average ATP content of mitochondria of the nematode strain of the control group is 9597.8 RLU/. Mu.g protein, compared with the nematode control group, the ATP production level of mitochondria treated by PVP-Pd NPs is obviously increased, and the average ATP content is 68111.6 RLU/. Mu.g protein. The results show that PVP-Pd NPs can obviously improve the mitochondrial productivity of the aged individuals and improve the respiratory function of the mitochondria. Therefore, the PVP-Pd NPs can be used as an effective mitochondrial activator and have important application prospects.
Example 4
Citrate synthasects-1Hexokinase, and a process for producing the samehxk-1And pyruvate carboxylasepyc-1Is a key enzyme involved in mitochondrial carbohydrate synthesis or glycolytic metabolism and is important for the exertion of mitochondrial respiratory function. Adding PVP-Pd NPs and FUDR into an escherichia coli OP50 bacterial solution, uniformly mixing, uniformly coating the mixture on an NGM flat plate to obtain an NGM flat plate containing PVP-Pd NPs and FUDR (the final concentration of the PVP-Pd NPs is 0.5 mu g/mL, the final concentration of the FUDR is 80 mu M, and the final concentration of the FUDR is used for inhibiting the oviposition of caenorhabditis elegans), adding FUDR into the escherichia coli OP50 bacterial solution, uniformly mixing, and uniformly coating the mixture on the NGM flat plate to obtain an NGM flat plate (the final concentration of the FUDR is 80 mu M) containing only FUDR and not containing PVP-Pd NPs; carrying out nematode synchronization treatment by adopting a wild caenorhabditis elegans model, and transferring a part of L4-stage nematode strains to an NGM flat plate containing PVP-Pd NPs and FUDR as an administration group when L1-stage wild type N2 caenorhabditis elegans grows to L4 stage; transferring another part of L4-stage nematode strains to NGM plates which do not contain PVP-Pd NPs and only contain FUDR to serve as a control group, culturing 50-100 nematodes in an incubator at 20 ℃ for 48h, collecting the nematodes treated by the control group and the PVP-Pd NPs, washing the nematodes with M9 buffer solution for three times, adding 1mL of TRIZOL (purchased from Invitrogen biotechnology company) into each nematode tube, splitting the nematodes and extracting mRNA; cDNA was synthesized using M-MLV reverse transcriptase (purchased from Invitrogen Biotech, product number # 28025013), and citrate synthase was determined by real-time PCR (q-PCR) using SYBR Green Supermix and q-PCR primer pairscts-1Hexokinasehxk-1And pyruvate carboxylasepyc-1The mRNA transcription expression level of (a).
PVP-Pd NPs on nematode mitochondrial citrate synthasects-1Hexokinasehxk-1And pyruvate carboxylasepyc- 1Relative reference geneactinThe results are shown in FIG. 5.
From FIG. 5As can be seen, feeding the nematodes with PVP-Pd NPs increased mitochondrial expression levels of metabolic enzymes in the nematodes relative to controls; the specific expression is that the PVP-Pd NPs treatment group can promote glucose metabolism enzyme after feeding the nematodes in the L4 stage for two dayscts-1Glucose metabolizing enzymehxk-1And glucose synthase pyc-1 were up-regulated 2-fold, 1.5-fold and 1.6-fold, respectively, relative to the reference gene (P)<0.05 This shows that PVP-Pd NPs fed the nematode can effectively improve the metabolic function of mitochondria.
Example 5
Adding PVP-Pd NPs and FUDR into an escherichia coli OP50 bacterial solution, uniformly mixing, uniformly coating the mixture on an NGM flat plate to obtain an NGM flat plate containing PVP-Pd NPs and FUDR (the final concentration of the PVP-Pd NPs is 0.5 mu g/mL, the final concentration of the FUDR is 80 mu M, and the final concentration of the FUDR is used for inhibiting the oviposition of caenorhabditis elegans), adding FUDR into the escherichia coli OP50 bacterial solution, uniformly mixing, and uniformly coating the mixture on the NGM flat plate to obtain an NGM flat plate (the final concentration of the FUDR is 80 mu M) containing only FUDR and not containing PVP-Pd NPs; carrying out nematode synchronization treatment by adopting a wild caenorhabditis elegans model, and transferring a part of L4-stage nematode strains to an NGM flat plate containing PVP-Pd NPs and FUDR as an administration group when L1-stage wild type N2 caenorhabditis elegans grows to the L4 stage; transferring another part of L4-stage nematode strains to NGM plates which do not contain PVP-Pd NPs and only contain FUDR to serve as a control group, culturing in an incubator at 20 ℃, wherein each group contains about 50-100 nematodes until adult nematodes die, counting the number of dead nematodes every other day by using an electron microscope, and removing the dead nematodes from the culture dish until all nematodes die; summarizing data, drawing a survival curve by Graph Pad, calculating a significance difference (Log-rank test) and counting the life span of the nematodes. The data of the life test are shown in Table 1, the results of the average life are shown in FIG. 6, and the results of the survival rate are shown in FIG. 7.
As can be seen from FIGS. 6 and 7, the mean life span of the nematodes in the control group was approximately 16.6 days, and the 0.5. Mu.g/mL PVP-Pd NPs-treated group significantly extended the life span of the nematodes, which was 19.7 days, up to 16.7% longer than the control group (P < 0.001). The number of days of nematode death in the PVP-Pd NPs-treated group was relatively delayed compared to the control group, and the longest life span of the nematodes was 30 days, while the longest life span of the nematodes in the control group was 24 days, and the longest life span of the nematodes in the PVP-Pd NPs-treated group was 6 days later than that in the control group. The result shows that the PVP-Pd NPs can obviously prolong the service life and have good anti-aging effect.
TABLE 1
Example 6
Adding PVP-Pd NPs and FUDR into an escherichia coli OP50 bacterial solution, uniformly mixing, uniformly coating the mixture on an NGM flat plate to obtain an NGM flat plate containing PVP-Pd NPs and FUDR (the final concentration of the PVP-Pd NPs is 0.5 mu g/mL, the final concentration of the FUDR is 80 mu M, and the final concentration of the FUDR is used for inhibiting the oviposition of caenorhabditis elegans), adding FUDR into the escherichia coli OP50 bacterial solution, uniformly mixing, and uniformly coating the mixture on the NGM flat plate to obtain an NGM flat plate (the final concentration of the FUDR is 80 mu M) containing only FUDR and not containing PVP-Pd NPs; carrying out nematode synchronization treatment by adopting a wild caenorhabditis elegans model, and transferring a part of L4-stage nematode strains to an NGM flat plate containing PVP-Pd NPs and FUDR as an administration group when L1-stage wild type N2 caenorhabditis elegans grows to the L4 stage; transferring another part of L4-stage nematode strains to NGM plates which do not contain PVP-Pd NPs and only contain FUDR as a control group, culturing in an incubator at 20 ℃ until the 1 st day or the 7 th day of adults, detecting lipofuscin fluorescence images (excitation: 365 nm; emission: 420 nm) of the nematodes by using a laser confocal imaging method, randomly shooting about 10 nematodes in each experimental group, and quantitatively analyzing lipofuscin in each nematode by using Image J software to obtain the lipofuscin fluorescence intensity of the nematodes, wherein the data are shown in figure 9.
Lipofuscin, an undegradable lysosome substance, is one of the useful biomarkers of caenorhabditis elegans physiological age, and its accumulation intensity is correlated with the age of the nematode. As shown in fig. 8, PVP-Pd NPs fed nematodes significantly decreased the intestinal lipofuscin content of adult nematodes on days 1 and 7; specifically, in the control group, part of luminous lipofuscin is observed in the intestinal tract of the nematode on the first day of the adult, and the content of the lipofuscin in the intestinal tract of the adult on the 7 th day is obviously increased relative to the content of the lipofuscin on the first day of the adult; in the PVP-Pd NPs treatment group, the lipofuscin content of the adult insects at day 1 and day 7 is far less than that of the adult insects at day 1 and day 7 in the control group respectively. The relative quantitative data (as shown in fig. 9) also demonstrate that, in particular, the mean fluorescence intensity of the intestinal lipofuscin of the adult nematodes at day 1 was 20.4 and the mean fluorescence intensity of the intestinal lipofuscin of the adult nematodes at day 7 was 44.6 in the control group, indicating that the lipofuscin content increased with the age of the nematodes. The average fluorescence intensity of intestinal lipofuscin of the nematodes at day 1 of the PVP-Pd NPs treatment group is 12.3, which is reduced by 40% compared with the control group (day 1 of the adults); on day 7 of PVP-Pd NPs treatment of the adult worms, the mean fluorescence intensity of intestinal lipofuscin in the nematodes was 37.1, which was a 17% reduction relative to the control (day 7 of adult worms). The results show that the PVP-Pd NPs can reduce the content of lipofuscin in the intestinal tracts of organisms of different age groups, and have a good anti-aging effect.
Example 7
The movement of caenorhabditis elegans is related to the strength of muscle tissues of the caenorhabditis elegans, and the influence of materials on the movement capability and the body function of the caenorhabditis elegans can be reflected by detecting the swing behavior of the caenorhabditis elegans. In order to further evaluate the influence of PVP-Pd NPs on nematode health parameters, the body swing ability of the nematodes was tested. Adding PVP-Pd NPs and FUDR into an escherichia coli OP50 bacterial solution, uniformly mixing, uniformly coating the mixture on an NGM flat plate to obtain an NGM flat plate containing PVP-Pd NPs and FUDR (the final concentration of the PVP-Pd NPs is 0.5 mu g/mL, the final concentration of the FUDR is 80 mu M, and the final concentration of the FUDR is used for inhibiting the oviposition of caenorhabditis elegans), adding FUDR into the escherichia coli OP50 bacterial solution, uniformly mixing, and uniformly coating the mixture on the NGM flat plate to obtain an NGM flat plate (the final concentration of the FUDR is 80 mu M) containing only FUDR and not containing PVP-Pd NPs; carrying out nematode synchronization treatment by adopting a wild caenorhabditis elegans model, and transferring a part of L4-stage nematode strains to an NGM flat plate containing PVP-Pd NPs and FUDR as an administration group when L1-stage wild type N2 caenorhabditis elegans grows to the L4 stage; transferring another part of L4-stage nematode strains to NGM plates which do not contain PVP-Pd NPs and only contain FUDR as a control group, culturing about 50-100 nematodes in each group at 20 ℃, collecting caenorhabditis elegans on the 1 st, 7 th and 10 th days after continuous administration, and recording the head swing times of the nematodes within 20s; after counting the data, significant differences were calculated using Graph Pad (Two-way ANOVA, sidak multiple complexes test), the error bars identify the mean Standard Error (SEM), and the results are shown in fig. 10.
As can be seen from FIG. 10, after 2 days of administration, i.e., day 1 of the administration, the head swing phenomenon was easily detected by the nematodes of the control group, the average number of head swings within 20s was 16.7 for the nematodes of the control group, and the average number of pharyngeal pumping was 17.4 for the nematodes of the PVP-Pd NPs administration group; compared with a blank group, the PVP-Pd NPs administration group has no obvious difference, and the PVP-Pd NPs are proved to have biological safety. On the 7 th day and the 10 th day of the adult, the number of head swings of the nematodes in the control group is obviously reduced relative to that of the adult on the 1 st day, and the number of head swings is 10.5 times and 9.4 times respectively in average every 20s times; the decrease range of the head swing quantity of the nematodes in the PVP-Pd NPs administration group is smaller, and the average 20s times is 12.1 times and 11.8 times respectively; compared with a control group, the PVP-Pd NPs administration group improves the head swing capacity of the nematodes on the 7 th day and the 10 th day, and has a significant difference with the control group. The results show that the PVP-Pd NPs have better biological safety, are beneficial to improving the swing capacity of the head and have the effect of delaying the decline of the exercise capacity related to aging.
Example 8
The pharynx undergoes degenerative decline with aging, and the feeding ability of the pharynx is continuously reduced, and the feeding ability of the pharynx can be usually judged by the movement frequency of the nematode pharyngeal pump. The process for detecting the swallowing frequency of the nematode comprises the following steps: adding PVP-Pd NPs and FUDR into an escherichia coli OP50 bacterial solution, uniformly mixing, uniformly coating the mixture on an NGM flat plate to obtain an NGM flat plate containing PVP-Pd NPs and FUDR (the final concentration of the PVP-Pd NPs is 0.5 mu g/mL, the final concentration of the FUDR is 80 mu M, and the final concentration of the FUDR is used for inhibiting the oviposition of caenorhabditis elegans), adding FUDR into the escherichia coli OP50 bacterial solution, uniformly mixing, and uniformly coating the mixture on the NGM flat plate to obtain an NGM flat plate (the final concentration of the FUDR is 80 mu M) containing only FUDR and not containing PVP-Pd NPs; carrying out nematode synchronization treatment by adopting a wild caenorhabditis elegans model, and transferring a part of L4-stage nematode strains to an NGM flat plate containing PVP-Pd NPs and FUDR as an administration group when L1-stage wild type N2 caenorhabditis elegans grows to the L4 stage; transferring another part of L4-stage nematode strains to NGM plates which do not contain PVP-Pd NPs and only contain FUDR to serve as a control group, culturing about 50-100 nematodes in each group at 20 ℃, collecting caenorhabditis elegans on the 1 st, 7 th and 10 th days of adult nematodes after continuous administration, observing nematode video under a stereoscopic microscope and counting the number of times of a 30-s endopharyngeal pumping, and recording 10 parallel times in each group. Data were analyzed by One-way ANOVAY statistics and data results are expressed as Mean + -SEM, as shown in FIG. 11.
As can be seen from FIG. 11, the average number of pharyngeal pumps was 123 for the control group nematode and 133 for the PVP-Pd NPs administration group nematode on the first day of adult; on the 7 th day of adult nematode, the average number of pharyngeal pumping of the nematode in the control group is 82, and the average number of pharyngeal pumping of the nematode in the PVP-Pd NPs administration group is 97; on day 10 of adult, the average number of pharyngeal pumping was 71 for the control group nematodes and 85 for the PVP-Pd NPs administration group. As can be seen, the PVP-Pd NPs administration group can obviously improve the pharyngeal swallowing frequency of the caenorhabditis elegans adults at 1 day, 7 days and 10 days. The results show that PVP-Pd NPs are helpful for improving the pharyngeal motility and have the effect of delaying the decline of the motility related to aging.
Example 9
Adding PVP-Pd NPs and FUDR into Escherichia coli OP50 bacterial liquid, mixing, uniformly coating on an NGM flat plate to obtain an NGM flat plate containing PVP-Pd NPs and FUDR (the final concentration of PVP-Pd NPs is 0.5 mu g/mL; the final concentration of FUDR is 80 mu M, and is used for inhibiting the oviposition of caenorhabditis elegans), adding FUDR into Escherichia coli OP50 bacterial liquid, mixing, and uniformly coating on an NGM flat plate to obtain an NGM flat plate containing only FUDR and not containing PVP-Pd NPs (the final concentration of FUDR is 80 mu M); carrying out nematode synchronization treatment by adopting a wild caenorhabditis elegans model, and transferring a part of L4-stage nematode strains to an NGM flat plate containing PVP-Pd NPs and FUDR as an administration group when L1-stage wild type N2 caenorhabditis elegans grows to the L4 stage; transferring another part of L4-stage nematode strains to NGM plates which do not contain PVP-Pd NPs and only contain FUDR as a control group, culturing about 50-100 nematodes in each group at 20 ℃, collecting the nematodes at 7 days of adult worms, transferring to NGM plates without PVP-Pd NPs for detecting the acute oxidative stress capacity of caenorhabditis elegans: culturing caenorhabditis elegans in a constant-temperature incubator at 35 ℃ for 3h, transferring to a blank plate, placing on a constant-temperature incubator at 20 ℃ for recovering for 1h, evaluating the survival rate of the caenorhabditis elegans, and judging the death standard in the same way as in example 5; evaluating the motor behavior ability of the survival nematodes, and recording the body bending times of the nematodes within 20s (the distance of one wavelength for the nematodes to climb forwards is recorded as one body bending); statistical data, significant differences were calculated using Graph Pad (Two-way ANOVA, sidak multiple complexes test), error bars indicate Standard Error of Mean (SEM), and the results are shown in fig. 12.
As can be seen from fig. 12, the results of the acute heat stress experiment show that the survival rate of the nematodes in the control group after the nematodes are treated at 35 ℃ for 3h on the seventh day of the adult nematodes is 20.4%, and the body bending frequency of the surviving nematodes is 2.6 times/20 s; the survival rate of the nematodes in the PVP-Pd NPs administration group after the same treatment is 53.3%, and the body bending frequency of the surviving nematodes is 3.3 times/20 s, which have significant differences. The result shows that the PVP-Pd NPs can obviously improve the acute hot stress capability of the nematodes, and further the PVP-Pd NPs can be applied to the preparation of products for improving the acute hot stress capability of organisms.
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, many simple modifications can be made to the technical solution of the invention, including combinations of various technical features in any other suitable way, and these simple modifications and combinations should also be regarded as the disclosure of the invention, and all fall within the scope of the invention.
Claims (9)
1. Application of palladium nanoparticles modified by polyvinylpyrrolidone in preparing products for resisting aging and/or enhancing heat stress resistance.
2. The use according to claim 1, wherein the anti-aging and/or anti-heat stress enhancing product is selected from at least one of a pharmaceutical, a nutraceutical and a food additive.
3. Application of palladium nanoparticles modified by polyvinylpyrrolidone in preparing products for improving mitochondrial respiration function.
4. The use according to claim 3, wherein the mitochondrial respiratory function improving product is selected from at least one of a pharmaceutical, a nutraceutical and a food additive.
5. Application of palladium nanoparticles modified by polyvinylpyrrolidone in preparing products for enhancing expression of mitochondrial metabolic enzymes.
6. The use according to claim 5, wherein the product that enhances expression of mitochondrial metabolic enzymes is selected from at least one of a pharmaceutical, a nutraceutical, and a food additive.
7. Use according to claim 5, characterized in that the mitochondrial metabolizing enzyme is selected from the group consisting of citrate synthasescts-1Hexokinase, and a process for producing the samehxk-1And pyruvate carboxylasepyc-1At least one of (a).
8. Use according to any one of claims 1 to 7, wherein the polyvinylpyrrolidone-modified palladium nanoparticles have a particle size of 15-25nm.
9. The use according to any one of claims 1 to 7, wherein the polyvinylpyrrolidone-modified palladium nanoparticles have a molar ratio of polyvinylpyrrolidone to palladium of 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211075886.7A CN115137837B (en) | 2022-09-05 | 2022-09-05 | Application of polyvinylpyrrolidone modified palladium nanoparticles |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211075886.7A CN115137837B (en) | 2022-09-05 | 2022-09-05 | Application of polyvinylpyrrolidone modified palladium nanoparticles |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115137837A CN115137837A (en) | 2022-10-04 |
| CN115137837B true CN115137837B (en) | 2022-11-25 |
Family
ID=83415799
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211075886.7A Active CN115137837B (en) | 2022-09-05 | 2022-09-05 | Application of polyvinylpyrrolidone modified palladium nanoparticles |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115137837B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118649245A (en) * | 2024-08-16 | 2024-09-17 | 北京市疾病预防控制中心 | Use of polyvinylpyrrolidone-modified palladium nanoparticles for preparing products for treating huntington's disease |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4511361B2 (en) * | 2002-04-26 | 2010-07-28 | ミズ株式会社 | Method and apparatus for quantitative analysis of dissolved hydrogen concentration in test water |
| JP4653945B2 (en) * | 2003-10-24 | 2011-03-16 | ミズ株式会社 | Pharmacologically functional water and its use |
-
2022
- 2022-09-05 CN CN202211075886.7A patent/CN115137837B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN115137837A (en) | 2022-10-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN115137837B (en) | Application of polyvinylpyrrolidone modified palladium nanoparticles | |
| TWI719691B (en) | Use of lactobacillus reuteri gkr1 for preparing composition of reducing uric acid | |
| EP3277287B1 (en) | Mixtures of human milk oligosaccharides comprising 3 '-o-sialyllactose | |
| JP2007507214A (en) | Analysis of prebiotic effects | |
| WO2023216952A1 (en) | Anti-ageing use of uridylic acid, adenylic acid, and yeast peptide composition | |
| Nie et al. | Multi-function screening of probiotics to improve oral health and evaluating their efficacy in a rat periodontitis model | |
| CN112274577B (en) | Use of a composition comprising a fermentation of a Musa species and a probiotic composition | |
| CN109912726B (en) | Phellinus linteus polysaccharide derivative, preparation method and application thereof | |
| EP2508190B1 (en) | Macrophage phagocytosis-activating composition and/or composition promoting cytokine production in macrophages | |
| CN118019840B (en) | Lactobacillus reuteri with functions of prolonging service life, resisting aging and reducing fat, and product and application thereof | |
| CN115192605B (en) | Application of cobaltosic oxide nanoparticles modified by dimercaptosuccinic acid | |
| JP2007267720A (en) | METHOD FOR PRODUCING PURIFIED beta-1,3-1,6-D-GLUCAN | |
| Budiasih et al. | Antihyperglicemic activity of some chromium (III)-amino acid complexes in nicotinamide-streptozotocin induced diabetic wistar rats | |
| KR101302043B1 (en) | Chitosan Oligosaccharide comprising of deep sea water for detoxicating alcohol or eliminating alcoholic | |
| CN107254461A (en) | A kind of carrier immobilized endoinulase of mesoporous silicon oxide and its application in FOS production | |
| CN114836336A (en) | Lactobacillus and application thereof | |
| CN113447462B (en) | Application of metformin-derived carbon quantum dots | |
| WO2005077390A1 (en) | Hypoglycemic agent, remedy/preventive for diabetes and proces for producing the same | |
| CN114532540B (en) | Maltopentaosyl trehalose and application of microsphere thereof in regulating intestinal flora | |
| CN114376983B (en) | Natural extract composite granule suitable for high uric acid population | |
| CN113817788B (en) | Enzymatic preparation method of glucosamine | |
| CN113929762B (en) | 3-hydroxybutyrylated and/or 3-hydroxypentylglycolylated modified insulin and application thereof | |
| CN115927345A (en) | DNA nano material targeting HIF-1 alpha and preparation method and application thereof | |
| CN109043224A (en) | A kind of cleanser reducing cadmium enrichment in edible shellfish body | |
| CN118203108A (en) | Tea polyphenol oxidation self-assembled nano biological material and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |