CN115127857A - Plant tissue sampling device - Google Patents
Plant tissue sampling device Download PDFInfo
- Publication number
- CN115127857A CN115127857A CN202210857539.3A CN202210857539A CN115127857A CN 115127857 A CN115127857 A CN 115127857A CN 202210857539 A CN202210857539 A CN 202210857539A CN 115127857 A CN115127857 A CN 115127857A
- Authority
- CN
- China
- Prior art keywords
- punching
- plant tissue
- centrifuge tube
- fixing
- sampling device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000005070 sampling Methods 0.000 title claims abstract description 44
- 238000004080 punching Methods 0.000 claims abstract description 80
- 238000003825 pressing Methods 0.000 claims abstract description 47
- 241000196324 Embryophyta Species 0.000 claims description 32
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 9
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 9
- 241001330002 Bambuseae Species 0.000 claims description 9
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 9
- 239000011425 bamboo Substances 0.000 claims description 9
- 230000000452 restraining effect Effects 0.000 claims 1
- 238000000034 method Methods 0.000 description 8
- 238000000605 extraction Methods 0.000 description 5
- 230000002349 favourable effect Effects 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 4
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a plant tissue sampling device, comprising: the base, the centrifuge tube rack, mount and perforating device, this application has set up the base of taking U type standing groove, and set up the spout supporting with the centrifuge tube rack on U type standing groove upper portion, set up mount and perforating device above U type standing groove, before the sampling operation, can place the centrifuging tube standing groove on the centrifuge tube rack with the centrifuge tube, and place plant tissue such as blade in the centrifuging tube top, through pressing perforating device, can punch the sample to plant tissue such as blade, and the plant tissue who takes out directly falls in the centrifuging tube, need not artifical the transfer, convenient operation is swift. Still can carry out multiunit sample operation simultaneously through removing the centrifuge tube rack, further raise the efficiency. The device can be matched with centrifuge tube racks with different specifications and punching devices with different specifications, can meet the sampling requirements of different centrifuge tubes for different tests, and can also be matched with punching devices with different quantities to further improve the sampling efficiency.
Description
Technical Field
The invention relates to the technical field of plant tissue sampling devices, in particular to a plant tissue sampling device.
Background
In the plant genome extraction test, the subsequent experimental operations such as separation and extraction can be carried out after a tissue sample is collected and put into a centrifugal tube. There are two cases when sampling: 1. directly taking a leaf sample in a field or in the field and putting the leaf sample into a centrifugal tube; 2. the leaves are processed (e.g., lyophilized or air dried) and placed into centrifuge tubes in a laboratory. Generally, the blade sample put into the centrifuge tube needs to be processed into a size of 2mm-5 mm. In laboratory sampling and subpackaging, a puncher is generally adopted for sampling. Traditional hole puncher does not have the device of fixed centrifuging tube, has beaten the hole sample back, puts into the centrifuging tube again, complex operation, and just confuses different experimental groups easily.
On the other hand, the tissue sample punched by the traditional puncher has only one specification, can not meet the requirements of different types of genome tests, and can not be adapted to centrifuge tubes with different apertures.
Disclosure of Invention
Aiming at the defects, the invention provides a plant tissue sampling device, which solves the problems of complicated sampling operation and low efficiency in the existing plant genome extraction test.
In order to achieve the purpose, the invention adopts the following technical scheme:
a plant tissue sampling device, comprising:
the device comprises a base, a first fixing device and a second fixing device, wherein a U-shaped placing groove is formed in the base, a sliding groove is formed in the upper portion of the U-shaped placing groove, and the sliding groove is horizontally arranged;
the centrifugal pipe rack is erected at the sliding groove at two sides and can horizontally slide relative to the sliding groove, and centrifugal pipe placing holes are formed in the centrifugal pipe rack at intervals;
the fixing frame is arranged above the base and the centrifuge tube rack, and fixing holes are formed in the fixing frame;
the punching device is erected on the fixing frame and penetrates through the fixing hole; the perforating device comprises a limiting cylinder, a connecting pipe, a limiting block, a perforating knife, a pressing rod and a pressing block, wherein the connecting pipe is of a hollow structure, the connecting pipe runs through the fixed hole, one end of the connecting pipe is connected with the limiting cylinder, the other end of the connecting pipe is connected with the limiting block, a via hole is formed in the limiting block, and is located above the fixed hole, a through hole is formed in the bottom opening and the top of the limiting cylinder, the pressing rod is located in the connecting pipe and runs through the via hole of the limiting block, the perforating hole of the limiting cylinder, one end of the pressing rod is connected with the perforating knife, the other end of the pressing rod is connected with the pressing block, the perforating knife is located in the limiting cylinder, a spring is further sleeved on the periphery of the pressing rod, and the spring is located between the pressing block and the limiting block or between the perforating knife and the limiting cylinder.
Preferably, the punching device further comprises a pushing block, the pushing block is connected with the inner side of the top of the punching knife through a spring, and the end of the pushing block is flush with the cutting edge of the punching knife.
Preferably, the perforating knife is a hollow cylinder with an opening at one end, and the cutting edge of the perforating knife is serrated.
Preferably, the height of the punching knife is smaller than that of the limiting cylinder.
Preferably, the end of the pressing rod is provided with a mounting hole with internal threads, the top end of the punching knife is fixed with a connecting part with external threads, and the punching knife is detachably connected with the pressing rod through the connecting part.
Preferably, be equipped with the fixed slot on the mount, the fixed orifices is located in the fixed slot, the shape and the size of fixed slot with perforating device's stopper phase-match, the stopper is located fixed slot department, and the stopper bottom with be equipped with the elastic component between the fixed slot bottom, the elasticity of elastic component is less than press the elasticity of the spring of depression bar periphery.
Preferably, a plurality of perforating devices are erected on the fixing frame.
Preferably, the U-shaped placing groove and the centrifuge tube shelf are also provided in plurality.
Preferably, the inner side of the centrifugal tube placing hole is provided with an elastic gasket. The elastic gasket is favorable for placing the centrifuge tube and is also adaptive to the centrifuge tubes with different apertures.
Preferably, a positioning line is drawn on the centrifugal tube rack.
Compared with the prior art, the invention has the beneficial effects that:
1. this plant sampling device has set up the base of taking U type standing groove, and set up the spout supporting with the centrifuge tube rack on U type standing groove upper portion, set up mount and perforating device in U type standing groove top, before the sampling operation, can place the centrifuge tube on the centrifuge tube rack, and place plant tissue such as blade in the centrifuge tube top, through pressing down perforating device, can punch the sample to plant tissue such as blade, and the plant tissue who takes out directly falls in the centrifuge tube, need not artifical the transfer, convenient operation is swift. Still can carry out multiunit sampling operation simultaneously through removing the centrifuge tube rack, further raise the efficiency.
2. This sampling device's perforating device includes spacing section of thick bamboo, connecting pipe, stopper, the sword of punching, presses the depression bar, presses structure such as briquetting, spring, and during sampling operation, spacing section of thick bamboo moves down and presses plant tissues such as blade, and the sword of punching is punched again and is punched the sample to plant tissues such as blade after that, need not the manual work and press the leaf tissue, and is easy and simple to handle. The sampling device can also be simultaneously matched with two sets of or more punching devices, two or more punching devices can be simultaneously sampled every time, and the sampling efficiency is improved by more than one time.
3. The punching knife of the sampling device is internally provided with an elastic pushing block, and when the punching knife punches and samples the blade tissue, the pushing block can outwards push the cut blade tissue to a centrifugal tube, so that the sampling efficiency is further improved. Meanwhile, the pushing block is fixed with the punching knife through a spring, has certain elasticity and is not easy to damage the leaf tissue.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below.
FIG. 1 is a schematic view of a plant tissue sampling device according to the present invention.
FIG. 2 is a schematic view of a disassembled structure of the plant tissue sampling device of the present invention.
FIG. 3 is a schematic view of the connection structure of the punching apparatus of the present invention.
FIG. 4 is a cross-sectional view of the perforating apparatus of the present invention.
Fig. 5 is a schematic structural diagram of another embodiment of the present invention.
FIG. 6 is a cross-sectional view of a perforating apparatus according to another embodiment of the present invention. .
Wherein the labels shown in the figures are:
10. a base; 11. a U-shaped placing groove; 111. a chute;
20. a centrifuge tube rack; 21. placing a hole in a centrifuge tube; 22. positioning a line;
30. a fixed mount; 31. fixing the cross bar; 32. fixing the vertical rod; 33. a fixing hole; 34. a fixing groove;
40. a punching device; 41. a limiting cylinder; 42. a connecting pipe; 43. a limiting block; 44. a punching knife; 45. a pressing lever; 46. pressing the block; 47. a spring; 48. and a push block.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "inside" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or orientations or positional relationships where products of the present invention are conventionally placed in use, and are used only for convenience in describing the present invention and for simplification of description, but do not indicate or imply that the device or the element to which the present invention is directed must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it should also be noted that, unless otherwise explicitly stated or limited, the terms "disposed" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in a specific case to those of ordinary skill in the art.
Example 1
As shown in fig. 1-4, a plant tissue sampling device, comprising:
the base 10, be equipped with U type standing groove 11 on the base 10, 11 both sides upper portions of U type standing groove are equipped with spout 111 respectively, spout 111 level sets up. In this embodiment, there are two U-shaped placing grooves 11, and the two U-shaped placing grooves 11 may be set to have the same size or different sizes.
The centrifugal pipe rack 20 is characterized in that two sides of the centrifugal pipe rack 20 are erected at the sliding groove 111 and can horizontally slide relative to the sliding groove 111, and centrifugal pipe placing holes 21 are formed in the centrifugal pipe rack 20 at intervals. Centrifuge tube rack 20 and centrifuging tube place hole 21 can be provided with the multiple, satisfy the demand of placing of different specification centrifuging tubes.
The fixing frame 30 is arranged above the base 10 and the centrifuge tube rack 20, and the fixing frame 30 is provided with a fixing hole 33. In this embodiment, the fixing frame 30 includes a fixing cross rod 31 and a fixing vertical rod 32, the fixing hole 33 is disposed on the fixing cross rod 31, and two ends of the fixing cross rod 31 are fixed to the outer sides of the U-shaped placing grooves 11 through the fixing vertical rod 32. Further, there are two fixing holes 33. Of course, a greater number of fixing holes 33 may be provided.
The perforating device 40 is erected on the fixing frame 30 and penetrates through the fixing hole 33. In this embodiment, there are two punching devices 40.
In use, the centrifuge tube is inserted into the centrifuge tube placing hole 21 of the centrifuge tube rack 20 and fixed, and the centrifuge tube rack 20 is inserted into the slide groove 111 of the U-shaped placing groove 11. The blade to be sampled is placed over the centrifuge tube orifice. Centrifuge tube rack 20 is pushed to a hand, makes the mouth of pipe of centrifuging tube be located perforating device 40 below, removes the in-process, and another hand lifts perforating device 40, waits centrifuge tube rack 20 to remove the assigned position after, pushes down perforating device 40, makes perforating device 40 punch the sample to the blade, and the blade tissue who gets falls the centrifuging tube, and is easy and simple to handle swift. The centrifuge tube holder 20 is further moved to sample the other parts of the blades.
Specifically, the punching device 40 comprises a limiting cylinder 41, a connecting pipe 42, a limiting block 43, a punching knife 44, a pressing rod 45 and a pressing block 46, the connecting pipe 42 is a hollow structure, the connecting pipe 42 penetrates through the fixing hole 33, and one end of the connecting pipe 42 is connected with the limiting cylinder 41, the other end is connected with the limiting block 43, the limiting block 43 is provided with a via hole which is positioned above the fixing hole 33, the bottom of the limiting cylinder 41 is provided with an opening, the top is provided with a through hole, the pressing rod 45 is arranged in the connecting pipe 42, and penetrates through the through hole of the limiting block 43 and the through hole of the limiting cylinder 41, one end of the pressing rod 45 is connected with the punching knife 44, the other end is connected with the pressing block 46, the punching knife 44 is arranged in the limiting cylinder 41, a spring 47 is further sleeved on the periphery of the pressing rod 45, and the spring 47 is arranged between the pressing block 46 and the limiting block 43. That is, a certain distance is left between the pressing block 46 and the stopper 43, and during the punching operation, the pressing block 46, that is, the punching blade 44, is pressed downward to punch and sample the leaf tissue. In other embodiments, the spring 47 may also be disposed between the punching blade 44 and the limiting cylinder 41. The limiting cylinder 41 can protect the punching knife 44, and can also press blade tissues in the punching operation process, so that the punching operation is facilitated.
Specifically, the punching blade 44 is a hollow cylinder with an opening at one end, and the cutting edge of the punching blade 44 is serrated. Furthermore, the end of the pressing rod 45 is provided with a mounting hole with internal threads, the top end of the punching knife 44 is fixed with a connecting part with external threads, and the punching knife 44 is detachably connected with the pressing rod 45 through the connecting part. The height of the punching knife 44 is smaller than that of the limiting cylinder 41. In the operation process that punches promptly, spacing section of thick bamboo 41 contacts the blade tissue earlier, presses the blade tissue back well, and the sample of punching is carried out to the blade again to the sword 44 that punches, more is favorable to taking out of blade tissue. Preferably, the bottom of the limiting cylinder 41 can be sleeved with a rubber ring and the like, so that the contact area between the limiting cylinder 41 and the blade tissue can be increased, and the blade tissue can be prevented from being crushed by the limiting cylinder 41.
Preferably, the punching device 40 further comprises a pushing block 48, the pushing block 48 is connected with the inner side of the top of the punching blade 44 through a spring 47, and the end of the pushing block 48 is flush with the blade edge of the punching blade 44. That is, while the punching knife 44 punches and samples the leaf tissue, the pushing block 48 pushes the taken-out leaf tissue into the centrifugal tube, which is beneficial to improving the sampling efficiency of the leaf tissue.
Further, a fixing groove 34 is formed in the fixing frame 30, the fixing hole 33 is formed in the fixing groove 34, the shape and size of the fixing groove 34 are matched with the limiting block 43 of the punching device 40, the limiting block 43 is arranged at the position of the fixing groove 34, an elastic member is arranged between the bottom of the limiting block 43 and the bottom of the fixing groove 34, and the elastic force of the elastic member is smaller than that of the spring 47 at the periphery of the pressing rod 45. That is, before the punch 40 is pressed, the bottom of the stopper cylinder 41 of the punch 40 is spaced from the centrifuge tube rack 20. After the centrifuge tube rack 20 and the leaf tissue move to the designated position, the perforating device 40 is pressed, the elastic part in the fixing groove 34 is compressed, the perforating device 40 integrally moves downwards, and the bottom of the limiting cylinder 41 is contacted with the leaf tissue to fix the leaf tissue; the punch 40 is further pushed down, the spring 47 on the outer periphery of the pressing rod 45 is contracted, and the punching blade 44 punches and samples the leaf tissue.
Further, two punching devices 40 are mounted on the fixing frame 30. The two punching devices 40 are respectively positioned above the two U-shaped placing grooves 11. The perforating knives 44 associated with the two perforating devices 40 are of different sizes. Correspondingly, the diameters of the centrifuge tube placing holes 21 in the two centrifuge tube racks 20 are different. The perforating device 40 and the centrifuge tube with different apertures can be selected according to the requirement. Of course, the perforating knife 44 and the centrifuge tube rack 20 may be replaced as necessary.
Further, a positioning line 22 is drawn on the centrifuge tube holder 20. Is favorable for positioning the centrifuge tube in the operation process. In other embodiments, an infrared sensing device may be disposed at the U-shaped placing groove 11, and when the centrifuge tube reaches a predetermined position, a warning sound is emitted to prompt the operator to stop pushing the centrifuge tube rack 20.
Example 2
As shown in fig. 5-6, a plant tissue sampling device, comprising:
the base 10, be equipped with U type standing groove 11 on the base 10, 11 both sides upper portions of U type standing groove are equipped with spout 111 respectively, spout 111 level sets up.
The centrifugal tube rack 20 is characterized in that two sides of the centrifugal tube rack 20 are erected at the sliding groove 111 and can horizontally slide relative to the sliding groove 111, and centrifugal tube placing holes 21 are formed in the centrifugal tube rack 20 at intervals. The centrifuge tube rack 20 can be matched with various specifications, and can meet the requirements of different plant genome extraction tests.
The fixing frame 30 is arranged above the base 10 and the centrifuge tube rack 20, and the fixing frame 30 is provided with a fixing hole 33. In this embodiment, the fixing frame 30 includes a fixing cross rod 31 and a fixing vertical rod 32, the fixing hole 33 is disposed on the fixing cross rod 31, and two ends of the fixing cross rod 31 are fixed to the outer sides of the U-shaped placing grooves 11 through the fixing vertical rod 32. In this embodiment, the fixing rail 31 is further provided with a sliding groove, the fixing holes 33 are disposed on the sliding groove at intervals, and the mounting position of the punching device 40 can be selected as required.
The perforating device 40 is erected on the fixing frame 30 and penetrates through the fixing hole 33. In this embodiment, there is one of the punching devices 40. The punching device 40 can move along the sliding groove and can be installed by selecting a proper fixing hole 33.
The operation method of this embodiment is similar to that of embodiment 1, and in use, a centrifuge tube is inserted into the centrifuge tube placing hole 21 of the centrifuge tube rack 20 and fixed, and the centrifuge tube rack 20 is inserted into the chute 111 of the U-shaped placing groove 11. The blade to be sampled is placed over the centrifuge tube orifice. One hand pushes centrifuge tube rack 20, makes the mouth of pipe of centrifuging tube be located perforating device 40 below, removes the in-process, and another hand lifts perforating device 40, waits that centrifuge tube rack 20 to remove the assigned position after, pushes down perforating device 40, makes perforating device 40 punch the sample to the blade, and the blade tissue of getting falls the centrifuging tube, and is easy and simple to handle swift. The centrifuge tube rack 20 is moved further to sample the other parts of the blade.
Specifically, the punching device 40 comprises a limiting cylinder 41, a connecting pipe 42, a limiting block 43, a punching knife 44, a pressing rod 45 and a pressing block 46, the connecting pipe 42 is a hollow structure, the connecting pipe 42 penetrates through the fixing hole 33, and one end of the connecting pipe 42 is connected with the limiting cylinder 41, the other end is connected with the limiting block 43, the limiting block 43 is provided with a via hole which is positioned above the fixing hole 33, the bottom of the limiting cylinder 41 is provided with an opening, the top is provided with a through hole, the pressing rod 45 is arranged in the connecting pipe 42, and penetrates through the through hole of the limiting block 43 and the through hole of the limiting cylinder 41, one end of the pressing rod 45 is connected with the punching knife 44, the other end is connected with the pressing block 46, the punching knife 44 is arranged in the limiting cylinder 41, a spring 47 is further sleeved on the periphery of the pressing rod 45, and the spring 47 is arranged between the pressing block 46 and the limiting block 43. The punching device 40 in this embodiment has two punching devices 40, and the pressing blocks 46 of the two punching devices 40 are connected to form one large pressing block 46. I.e. by pressing one of the pressing blocks 46, two punching devices 40 can be operated simultaneously. Of course, in other embodiments, a greater number of punch devices 40 may be provided to further improve efficiency. In other embodiments, the centrifuge tube placing hole 21 is provided with an elastic gasket on the inner side, which is not only beneficial to placing the centrifuge tube, but also suitable for centrifuge tubes with different apertures.
Specifically, the punching blade 44 is a hollow cylinder with an opening at one end, and the cutting edge of the punching blade 44 is serrated. The blade can be cut off easily. Furthermore, the end of the pressing rod 45 is provided with a mounting hole with internal threads, the top end of the punching knife 44 is fixed with a connecting part with external threads, and the punching knife 44 is detachably connected with the pressing rod 45 through the connecting part. The height of the punching knife 44 is smaller than that of the limiting cylinder 41. In the operation process that punches promptly, spacing section of thick bamboo 41 contacts the blade tissue earlier, presses the blade tissue back well, and the sample of punching is carried out to the blade again to the sword 44 that punches, more is favorable to taking out of blade tissue. The bottom of the limiting cylinder 41 can be sleeved with a rubber ring sleeve, so that the contact area with the leaf tissue can be increased, and the leaf tissue can be prevented from being crushed by the limiting cylinder 41.
Preferably, the punching device 40 further comprises a pushing block 48, the pushing block 48 is connected with the inner side of the top of the punching blade 44 through a spring 47, the pushing block 48 is arranged in the middle of the inner side of the punching blade 44, and the end of the pushing block 48 is flush with the cutting edge of the punching blade 44. That is, while the punching knife 44 punches and samples the leaf tissue, the pushing block 48 pushes the taken leaf tissue into the centrifugal tube, which is beneficial to improving the sampling efficiency of the leaf tissue.
Further, a fixing groove 34 is formed in the fixing frame 30, the fixing hole 33 is formed in the fixing groove 34, the shape and size of the fixing groove 34 are matched with the limiting block 43 of the punching device 40, the limiting block 43 is formed in the fixing groove 34, an elastic member is arranged between the bottom of the limiting block 43 and the bottom of the fixing groove 34, and the elastic force of the elastic member is smaller than that of the spring 47 on the periphery of the pressing rod 45. That is, before the punch 40 is pressed, the bottom of the stopper cylinder 41 of the punch 40 is spaced from the centrifuge tube rack 20. After the centrifuge tube shelf 20 and the leaf tissue move to the designated positions, the perforating device 40 is pressed, the elastic part in the fixing groove 34 is compressed, the perforating device 40 integrally moves downwards, and the bottom of the limiting cylinder 41 is contacted with the leaf tissue to fix the leaf tissue; the punch 40 is further pushed down, the spring 47 on the outer periphery of the pressing rod 45 is contracted, and the punching blade 44 punches and samples the leaf tissue.
Further, a positioning line 22 is drawn on the centrifuge tube holder 20. Is favorable for positioning the centrifuge tube in the operation process. In other embodiments, a positioning strip with certain elasticity may be provided inside the U-shaped placement groove 11 for positioning, or an elastic protrusion and a groove may be provided between the centrifuge tube rack 20 and the U-shaped placement groove 11 for positioning, and after a certain external force is exceeded, the protrusion may move relative to the groove.
The leaf tissue sampling that is used for plant genome extraction test with this device, before the sample, place the hole 21 in centrifugal tube that inserts centrifugal tube into centrifuge tube rack 20 and fix to in inserting the spout 111 of U type standing groove 11 with centrifugal tube rack 20. The blade to be sampled is placed over the centrifuge tube orifice. A hand pushes the centrifuge tube rack 20 to enable the tube opening of the centrifuge tube to be positioned below the perforating device 40, after the centrifuge tube rack 20 moves to a designated position, the perforating device 40 is pressed downwards, the bottom of the limiting cylinder 41 is firstly contacted with the leaf tissue, and the leaf tissue is fixed; the perforating device 40 is continuously pressed downwards, the spring 47 on the periphery of the pressing rod 45 contracts, the perforating knife 44 perforates and samples the leaf tissue, and meanwhile, the pushing block 48 pushes the taken leaf tissue into the centrifugal tube, so that the operation is simple, convenient and quick. The centrifuge tube holder 20 is further moved to sample the other parts of the blades. The two perforating devices 40 are matched in the embodiment, two samples can be simultaneously sampled once each operation, and the sampling efficiency is doubled.
To sum up, this sampling device has set up the base 10 of taking U type standing groove 11, and set up the spout 111 supporting with centrifuge tube rack 20 on 11 upper portions of U type standing groove, set up mount 30 and perforating device 40 in 11 tops of U type standing groove, before the sampling operation, can place the centrifuging tube standing groove on centrifuge tube rack 20 with the centrifuge tube, and place plant tissue such as blade in the centrifuging tube top, through pressing perforating device 40, can punch the sample to plant tissue such as blade, and the plant tissue who takes out directly falls in the centrifuging tube, need not artifical the transfer, and convenient operation is swift. And a plurality of groups of sampling operations can be simultaneously carried out by moving the centrifuge tube rack 20, so that the efficiency is further improved. This device can be supporting the centrifuge tube rack 20 of different specifications, also can be supporting the perforating device 40 of different specifications, can satisfy the sample requirement of the different centrifuging tubes of different experiments, still can support the perforating device 40 of different quantity, further improves sample efficiency.
The above description is only for the specific embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (10)
1. A plant tissue sampling device, comprising:
the device comprises a base, wherein a U-shaped placing groove is formed in the base, a sliding groove is formed in the upper portion of the U-shaped placing groove, and the sliding groove is horizontally arranged;
the centrifugal pipe rack is erected at the sliding groove at two sides and can horizontally slide relative to the sliding groove, and centrifugal pipe placing holes are arranged on the centrifugal pipe rack at intervals;
the fixing frame is arranged above the base and the centrifuge tube rack, and fixing holes are formed in the fixing frame;
the punching device is erected on the fixing frame and penetrates through the fixing hole; perforating device includes spacing section of thick bamboo, connecting pipe, stopper, punching knife, presses the depression bar and presses the pressure piece, the connecting pipe is hollow structure, the connecting pipe runs through the fixed orifices, and connecting pipe one end with spacing section of thick bamboo links to each other, the other end with the stopper links to each other, be equipped with the via hole on the stopper, the via hole is located the fixed orifices top, spacing bobbin base portion opening, top are equipped with the perforation, press the depression bar to locate in the connecting pipe, and run through the via hole of stopper reaches the perforation of spacing section of thick bamboo, press down lever one end with punching knife links to each other, the other end with press the piece and link to each other, punching knife locates in the spacing section of thick bamboo, still the cover is equipped with the spring according to the depression bar periphery, the spring is located punch the piece with between the stopper, perhaps locate the sword with between the spacing section of thick bamboo.
2. The device for sampling plant tissue according to claim 1, wherein said punching means further comprises a pushing block connected to the inside of the top of said punching blade by a spring, the end of said pushing block being flush with the cutting edge of said punching blade.
3. The plant tissue sampling device according to claim 1, wherein the punching blade is a hollow cylinder having an opening at one end, and the cutting edge of the punching blade is serrated.
4. The plant tissue sampling device of claim 3, wherein the height of the perforating knife is less than the height of the restraining cylinder.
5. The plant tissue sampling device according to claim 3, wherein the end of the pressing rod is provided with a mounting hole with internal threads, the top end of the punching knife is fixed with a connecting part with external threads, and the punching knife is detachably connected with the pressing rod through the connecting part.
6. The plant tissue sampling device according to claim 1, wherein the fixing frame is provided with a fixing groove, the fixing hole is provided in the fixing groove, the fixing groove has a shape and size matched with the stopper of the punching device, the stopper is provided at the fixing groove, and an elastic member is provided between the bottom of the stopper and the bottom of the fixing groove, the elastic member having an elastic force smaller than that of the spring provided at the periphery of the pressing rod.
7. The plant tissue sampling device of claim 1, wherein a plurality of perforation devices are mounted on the mounting bracket.
8. A plant tissue sampling device according to claim 7 wherein a plurality of said U-shaped standing grooves and said centrifuge tube rack are also provided.
9. The plant tissue sampling device according to claim 1, wherein an elastic washer is provided inside the centrifugal tube placing hole.
10. The plant tissue sampling device of claim 1 wherein a positioning line is drawn on the centrifuge tube rack.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210857539.3A CN115127857A (en) | 2022-07-20 | 2022-07-20 | Plant tissue sampling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210857539.3A CN115127857A (en) | 2022-07-20 | 2022-07-20 | Plant tissue sampling device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115127857A true CN115127857A (en) | 2022-09-30 |
Family
ID=83384861
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210857539.3A Pending CN115127857A (en) | 2022-07-20 | 2022-07-20 | Plant tissue sampling device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115127857A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024108358A1 (en) * | 2022-11-21 | 2024-05-30 | 深圳先进技术研究院 | Tissue sample pusher |
-
2022
- 2022-07-20 CN CN202210857539.3A patent/CN115127857A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024108358A1 (en) * | 2022-11-21 | 2024-05-30 | 深圳先进技术研究院 | Tissue sample pusher |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR102235511B1 (en) | Apparatus for removing seed of fruit | |
| CN115127857A (en) | Plant tissue sampling device | |
| CN211891002U (en) | Traditional chinese medicine cutting device with adjustable | |
| CN112056570B (en) | Green orange processing device | |
| US2345731A (en) | Fruit juice extractor | |
| CN211220975U (en) | Fruit sectioning device | |
| CN212540196U (en) | Scanning electron microscope plant sample sampler | |
| CN217071590U (en) | Positioning device for cutting radiator section | |
| CN211362570U (en) | Finance text data perforating device | |
| CN1463216A (en) | Dual-purpose citrus fruit slicer | |
| CN213848650U (en) | Meat taking device for processing dried orange peel | |
| CN212978563U (en) | Ring knife type herbal medicine slicing machine | |
| CN212684087U (en) | Cigarette paper stripping off device | |
| CN113243779A (en) | Novel shrimp instrument of shelling | |
| CN207754494U (en) | The high cherry kernel removing device of dressing percentage | |
| CN208624566U (en) | A kind of fruit opens valve peeling machine | |
| CN217407700U (en) | Fresh olive fruit denucleation device | |
| CN216266207U (en) | Apple root and pedicle cutting equipment for agricultural apple processing | |
| CN219294850U (en) | Adjustable hole cleaning device for carton sheet blank | |
| CN217407273U (en) | Macro fungus strain separation device | |
| CN211333690U (en) | Raw bamboo slitting device | |
| CN213819756U (en) | Fruit cutting device for processing dried orange peel | |
| CN209665596U (en) | A kind of sample slicer | |
| CN220575383U (en) | Automatic cutting device for end face of stainless steel pipe blank | |
| CN215825385U (en) | Orange processing and slicing device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |