CN115096871B - Detection device applied to multichannel SERS micro-fluidic chip - Google Patents
Detection device applied to multichannel SERS micro-fluidic chip Download PDFInfo
- Publication number
- CN115096871B CN115096871B CN202210862608.XA CN202210862608A CN115096871B CN 115096871 B CN115096871 B CN 115096871B CN 202210862608 A CN202210862608 A CN 202210862608A CN 115096871 B CN115096871 B CN 115096871B
- Authority
- CN
- China
- Prior art keywords
- detection
- silver
- detected
- nano
- lipid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 122
- 238000004416 surface enhanced Raman spectroscopy Methods 0.000 title claims abstract description 60
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical group [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims abstract description 181
- 150000002632 lipids Chemical class 0.000 claims abstract description 120
- 238000001069 Raman spectroscopy Methods 0.000 claims abstract description 45
- 239000012488 sample solution Substances 0.000 claims abstract description 29
- 239000000758 substrate Substances 0.000 claims abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 57
- 229910052709 silver Inorganic materials 0.000 claims description 53
- 239000004332 silver Substances 0.000 claims description 53
- 229910001868 water Inorganic materials 0.000 claims description 48
- 239000012528 membrane Substances 0.000 claims description 35
- 239000000575 pesticide Substances 0.000 claims description 34
- 239000002356 single layer Substances 0.000 claims description 32
- 239000011521 glass Substances 0.000 claims description 21
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 20
- 239000003638 chemical reducing agent Substances 0.000 claims description 19
- 229910021607 Silver chloride Inorganic materials 0.000 claims description 18
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 claims description 18
- 239000010410 layer Substances 0.000 claims description 13
- 239000011148 porous material Substances 0.000 claims description 11
- 239000008367 deionised water Substances 0.000 claims description 10
- 229910021641 deionized water Inorganic materials 0.000 claims description 10
- 235000011164 potassium chloride Nutrition 0.000 claims description 10
- 239000001103 potassium chloride Substances 0.000 claims description 10
- 239000012279 sodium borohydride Substances 0.000 claims description 9
- 229910000033 sodium borohydride Inorganic materials 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 238000004140 cleaning Methods 0.000 claims description 8
- 238000003487 electrochemical reaction Methods 0.000 claims description 8
- 238000005516 engineering process Methods 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 7
- 230000001603 reducing effect Effects 0.000 claims description 5
- 238000003825 pressing Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000011065 in-situ storage Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 239000002120 nanofilm Substances 0.000 claims description 3
- 239000000243 solution Substances 0.000 description 36
- 230000004048 modification Effects 0.000 description 22
- 238000012986 modification Methods 0.000 description 22
- 238000000034 method Methods 0.000 description 15
- 239000000523 sample Substances 0.000 description 8
- 230000000875 corresponding effect Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000009826 distribution Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 230000003321 amplification Effects 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 238000011897 real-time detection Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000004454 trace mineral analysis Methods 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 239000007783 nanoporous material Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The application provides a detection device applied to a multichannel SERS microfluidic chip, which comprises a microfluidic pipeline and a detection chip; the detection chips comprise a plurality of detection chips, the detection chips are connected to the output pipeline end of the microfluidic pipeline in parallel, and the input pipeline end of the microfluidic pipeline is used for inputting a sample solution to be detected; each detection chip comprises an SERS substrate applied to detecting objects to be detected with different properties, and the SERS substrate comprises any one of the following nano porous silver structures: the surface of the nano porous silver structure is modified by a single lipid film, and the surface of the nano porous silver structure is modified by a double lipid film; the SERS substrate adsorbs molecules to be detected in the sample solution to be detected, and a Raman signal of the molecules to be detected adsorbed by the detection chip is detected through the handheld Raman spectrometer. The problem of the waiting of different properties wait to detect the thing and be difficult to detect simultaneously, detection efficiency is low is solved, realize multichannel simultaneous detection, improve detection efficiency.
Description
Technical Field
The application relates to the technical field of detection devices, in particular to a detection device applied to a multichannel SERS microfluidic chip.
Background
The Surface Enhanced Raman Scattering (SERS) technology is a novel trace analysis technology, can obtain information of influence of genes, chemical bonds and microenvironment on sample structures from a molecular level, and can obtain fingerprint spectrums of the sample structures in real time, has large information quantity, has the characteristics of high sensitivity, strong specificity, no marking, no damage, real-time detection and the like, cannot be interfered by media such as solution and the like, and is widely applied to the fields of biochemical detection and the like. The micro-fluidic chip is a core technology applied to the field of micro total analysis systems.
At present, a common method for combining the SERS technology with the microfluidic chip is improved from disposable detection to reusable, such as application number 201810524022.6, which discloses a reusable high-flux SERS microfluidic chip and applications thereof, including a reaction chip and a detection chip connected in series through a microfluidic pipeline; the reaction chip is used for mixing and reacting a liquid sample containing heavy metal ions with a DNA enzyme reagent; the detection chip is used for detecting the reactant after the reaction of the reaction chip. The application of the reusable high-flux SERS microfluidic chip is also provided, and the reusable high-flux SERS microfluidic chip is applied to detecting the content of trace harmful metal ions in a water environment. The designed SERS microfluidic chip can be repeatedly recycled, has simple process, low cost and high efficiency, and can be produced in batches. The method can detect a plurality of data at one time, and has the characteristics of rapidness, high efficiency and high throughput detection. Although the multi-channel detection device can be repeatedly used, the problems of low detection efficiency, weak signal intensity and the like due to different hydrophilic and lipophilic characteristics of different objects to be detected cannot be solved, and multiple types of detection can be simultaneously carried out.
Disclosure of Invention
The main purpose of this application is to provide a detection device who is applied to multichannel SERS micro-fluidic chip, aims at solving the problem that the thing that awaits measuring of different properties is difficult to detect simultaneously, detection efficiency hangs down.
In order to achieve the above object, the present application provides a detection apparatus applied to a multichannel SERS microfluidic chip, including a microfluidic pipeline and a detection chip;
the detection chips comprise a plurality of detection chips, the detection chips are connected to the output pipeline end of the microfluidic pipeline in parallel, and the input pipeline end of the microfluidic pipeline is used for inputting a sample solution to be detected;
each detection chip comprises an SERS substrate used for detecting objects to be detected with different properties, and the SERS substrate comprises any one of the following nano porous silver structures: the surface of the nano porous silver structure is modified by a monolayer lipid film, and the surface of the nano porous silver structure is modified by a double-layer lipid film;
the SERS substrate adsorbs molecules to be detected in the sample solution to be detected, and a Raman signal of the molecules to be detected adsorbed by the detection chip is detected through a handheld Raman spectrometer.
Further, the manufacturing process of the SERS substrate includes:
washing silver flakes with the purity of 99.9% by using double deionized water, and placing the washed silver flakes into potassium chloride water with a preset concentration to perform an electrochemical reaction to obtain silver chloride plated silver flakes;
stirring the solution in which the silver chips plated with the silver chloride are positioned, placing the silver chips plated with the silver chloride into a reducing agent, and reducing the silver chips plated with the silver chloride into nano-porous silver chips in situ through the reducing agent;
and washing the porous silver sheet by using deionized water and ethanol to obtain the nano porous silver structure.
Further, potassium chloride water was electrochemically reacted by 0.15 v.
Further, the diameter of each pore diameter in the nano-porous silver structure is 60-80nm.
Furthermore, the detection chips comprise three detection chips, namely a first detection chip, a second detection chip and a third detection chip;
the SERS substrate in the first detection chip adopts a nano porous silver structure with a surface modified by a single lipid film;
the SERS substrate in the second detection chip adopts a nano porous silver structure with the surface modified by double lipid films;
the SERS substrate in the third detection chip adopts the nano-porous silver structure.
Further, a single lipid film is deposited on the nano-porous silver structure through a technology of constructing an ordered ultrathin molecular film, so that the nano-porous silver structure with the surface modified by the single lipid film is obtained.
Further, the step of modifying the surface of the nano-porous silver structure to generate a monolayer lipid membrane comprises:
dropping a certain amount of lipid solution on the water surface through a Hamilton micro-injector until the lipid solution is evaporated to form a lipid membrane;
pressing the lipid membrane at a preset speed until the lipid membrane is subjected to a target surface pressure;
and (3) pulling up the glass slide positioned in the water and the nano porous silver structure positioned on the glass slide at a specified speed until the nano porous silver structure is exposed out of the water surface, thus obtaining the nano porous silver structure with the surface modified by the monolayer lipid film.
Further, the step of surface modification of the nanoporous silver structure to generate monolayer lipid membrane modification comprises:
dropping a certain amount of lipid solution on the water surface through a Hamilton micro-injector until the lipid solution is evaporated to form a lipid membrane;
pressing the lipid membrane at a preset speed until the lipid membrane bears a target surface pressure;
pulling up the glass slide positioned in the water and the nano porous silver structure positioned on the glass slide at a designated speed until the nano porous silver structure is exposed out of the water surface;
and inserting the nano-porous silver structure into the water again to realize the nano-porous silver structure with the surface modified by the double lipid films.
Further, the reducing agent is sodium borohydride solution.
Furthermore, an input pipeline end of the micro-fluidic pipeline is arranged to be a Y-shaped pipeline, a containing cavity where the sample solution to be detected is located is communicated with a first port of the Y-shaped pipeline, and a second port of the Y-shaped pipeline is communicated with a containing cavity where a cleaning solution for cleaning the micro-fluidic pipeline is located.
The detection device applied to the multi-channel SERS micro-fluidic chip provided in the application has the following beneficial effects:
through setting up a plurality of detection chips that connect in parallel, and set up the nano-porous structure that is used for detecting the molecule of waiting to detect that corresponds the nature on detecting the chip to solve the waiting of different properties and detect the thing and be difficult to detect simultaneously, detection efficiency hangs down the problem, realize the multichannel and detect simultaneously, improve detection efficiency.
Drawings
Fig. 1 is a schematic structural diagram of a detection apparatus applied to a multichannel SERS microfluidic chip according to an embodiment of the present application.
The implementation, functional features and advantages of the object of the present application will be further explained with reference to the embodiments, and with reference to the accompanying drawings.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the present application is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.
Referring to fig. 1, in an embodiment of the present application, a detection apparatus applied to a multichannel SERS microfluidic chip is provided, including a microfluidic pipeline 2 and a detection chip 1; the detection chip 1 comprises a plurality of detection chips, the detection chips are connected to the output pipeline end of the micro-fluidic pipeline 2 in parallel, and the input pipeline end of the micro-fluidic pipeline 2 is used for inputting a sample solution 3 to be detected; wherein, each detection chip 1 is including being applied to detecting the SERS basement that different properties detected the thing, and the SERS basement is including any one in following nanometer porous silver structure 5: the surface of the nano porous silver structure is subjected to monolayer lipid membrane modification 51, the surface of the nano porous silver structure is subjected to double-layer lipid membrane modification 52, and the surface of the nano porous silver structure is subjected to pure nano porous silver structure 53; the SERS substrate adsorbs molecules to be detected in a sample solution to be detected, and a Raman signal of the molecules to be detected adsorbed by the detection chip 1 is detected through the handheld Raman spectrometer.
The application is applied to detect waiting to detect the sample of waiting to detect different properties in waiting to detect liquid, more specifically is through decorating the surface of nanometer porous silver structure 5 to the realization is richened and is realized detecting waiting to detect the sample of different properties, if the sample that waits to detect of different properties includes at least one: hydrophilic pesticides, lipophilic pesticides, charged pesticides; the multi-channel detection of the object to be detected is realized, and the detection efficiency is improved.
In the embodiment, the microfluidic pipeline 2 is used for conveying a sample solution to be detected to an area to be detected, the microfluidic pipeline 2 comprises an input pipeline end and an output pipeline end, wherein the sample solution to be detected enters into the output pipeline end from the input pipeline end, the output pipeline end is connected in parallel with a plurality of detection chips 1, each detection chip 1 comprises an SERS substrate used for detecting objects to be detected with different properties, SERS refers to a surface enhanced Raman scattering technology and is a novel trace analysis technology, information of influence of genes, chemical bonds and micro-environments on a sample structure can be obtained from a molecular level, a fingerprint spectrum of the sample structure can be obtained in real time, the information amount is large, and the method has the characteristics of high sensitivity, strong specificity, no mark, no damage, real-time detection and the like, and cannot be interfered by media such as solution and the like; in this embodiment, the SERS substrate includes a nanoporous silver structure 5, specifically includes nanoporous silver structures 5 with different surface modifications, such as a nanoporous silver structure 51 with a single lipid film modified surface, a nanoporous silver structure 52 with a double lipid film modified surface, or a nanoporous silver structure 53; amplifying and displaying the nano porous silver structure 5 through an electron microscope to observe whether the pore form distribution of the nano porous silver structure 5 on the SERS substrate is uniform or not, or to observe whether the pore form distribution of the nano porous silver structure 5 modified by adding a lipid film is uniform or not; if the morphology distribution of the nano-porous silver structure 52 with the double-layer lipid film modified surface is uneven in the chip where the nano-porous silver structure 52 with the double-layer lipid film modified surface is observed through a high-magnification scanning electron microscope, the nano-porous silver structure 52 with the double-layer lipid film modified surface is uneven in pore shape, and the nano-porous silver structure is a defective product; if the morphology distribution of the nanoporous silver structure 51 with the surface modified by the monolayer lipid film is uniform in the chip where the nanoporous silver structure 51 with the surface modified by the monolayer lipid film is observed through a high-magnification scanning electron microscope, the nanoporous silver structure 51 with the surface modified by the monolayer lipid film is uniform in pore shape, and the product is good. The SERS base is used for adsorbing the molecules to be detected of waiting to detect sample solution, the Raman signal of the molecules to be detected of the nanoporous silver structure 5 on the detection chip 1 is detected through the hand-held Raman spectrometer, when adsorbing the molecules to be detected of corresponding attribute, then the Raman signal can be amplified to the signal of the sixth power of ten of original Raman signal, set up this nanoporous silver structure 5 in order to realize amplifying the Raman signal to adsorbed chemical molecule, and then look over whether there is the object to be detected of the different nature that needs detected in waiting to detect sample solution.
As in one embodiment, the detection chip 1 includes two detection chips 1, two detection chips 1 are connected in parallel at the end of the output pipeline, and one detection chip of the two detection chips 1 is a nano-porous silver structure 51 (i.e. the detection chip is the first detection chip 11) with a surface modified by a single lipid film, and is used for adsorbing and detecting the lipophilic pesticide; the other detection chip 1 is a nano-porous silver structure 52 (namely, the detection chip is a second detection chip 12) with a double-layer lipid membrane modified surface, and is used for adsorbing and detecting the charged pesticide; a sample solution to be detected is shunted to the two detection chips 1 from the output pipeline end, if a Raman signal of a molecule to be detected of the nano porous silver structure 5 positioned on the detection chips 1 is detected by a handheld Raman spectrometer, when the molecule to be detected with the corresponding property is adsorbed, if lipophilic pesticide is adsorbed in the first detection chip 11 where the nano porous silver structure 51 with the single-layer lipid membrane modified surface is positioned, the Raman signal is amplified to a signal which is six times of ten times of the original Raman signal, which indicates that the nano porous silver structure 51 with the single-layer lipid membrane modified surface adsorbs the lipophilic pesticide, namely the lipophilic pesticide exists in the sample solution to be detected; if the raman signal of the molecule to be detected of the nanoporous silver structure 5 on the detection chip 1 is detected by the handheld raman spectrometer, when the molecule to be detected with the corresponding property is adsorbed, if the charged pesticide is adsorbed in the second detection chip 12 where the nanoporous silver structure 52 with the surface modified by the bilayer lipid membrane is located, the raman signal is amplified to a signal which is six times of ten times of the original raman signal, which indicates that the nanoporous silver structure with the surface modified by the bilayer lipid membrane adsorbs the charged pesticide, that is, the charged pesticide exists in the sample solution to be detected. The multi-channel detection device for the molecules to be detected with different properties in the same sample solution to be detected realizes the simultaneous detection of the molecules to be detected with different properties, and improves the detection efficiency. In this embodiment, the magnification of the electron microscope is mainly the morphology of the nanoporous structure, and the specific magnification is not limited herein. In other embodiments, a plurality of parallel detection chips 1 for detecting different properties may also be provided, and the number of channels detected in parallel is not limited here.
In this embodiment, the manufacturing process of the SERS substrate includes:
step 1, washing silver flakes with the purity of 99.9% by using double deionized water, and placing the washed silver flakes into potassium chloride water with a preset concentration to perform an electrochemical reaction to obtain silver flakes plated with silver chloride;
step 2, stirring the solution in which the silver pieces plated with the silver chloride are positioned, placing the silver pieces plated with the silver chloride in a reducing agent, and reducing the silver pieces plated with the silver chloride into nano porous silver pieces in situ through the reducing agent;
and 3, washing the porous silver sheet by using deionized water and ethanol to obtain the nano porous silver structure 53.
In this embodiment, in step 1, a silver sheet with a purity of 99.9% is washed by using double deionized water (dd-H2O), and the washed silver sheet is placed in a potassium chloride aqueous solution electrolyte with a preset concentration to perform an electrochemical reaction, wherein the concentration of the potassium chloride aqueous solution can be preset to be 1 mol/l of potassium chloride aqueous solution, at this time, the surface of the metal silver sheet is changed from glossy silver color to dark yellow color, and the electrochemical reaction is taken by the user after the metal silver sheet is changed to dark yellow color, so that the silver sheet plated with silver chloride can be obtained; stirring the water solution in which the silver sheet plated with silver chloride is located to enable the silver sheet to be in the dynamically stirred liquid, then placing the silver sheet in a reducing agent, and reducing the silver sheet into a nano porous silver sheet through the original taste of the reducing agent, wherein the silver sheet quickly enters the reducing agent from the dynamically stirred liquid, the reducing agent can adopt a sodium borohydride solution, the silver sheet plated with silver chloride is reduced through the sodium borohydride solution, bubbles appear in the sodium borohydride solution at this time, and when the bubbles in the sodium borohydride solution are eliminated, the silver sheet plated with silver chloride is judged to be reduced into the nano porous silver sheet, and then washing is carried out through deionized water and ethanol to obtain the required nano porous silver structure.
The principle of generating the nano-porous silver structure 53 is to respectively oxidize pure silver with smooth and flat surface into silver chloride through electrochemical reaction, and generate the nano-porous silver structure through original taste reduction induction by a reducing agent.
In this embodiment, the reducing agent is a substance that loses electrons or deviates electrons in the redox reaction. The reducing agent itself has reducing properties and is oxidized, and the product is called an oxidation product. The reduction and the oxidation reaction are carried out simultaneously, that is, the reducing agent itself is oxidized to become an oxide while the reducing agent is oxidized with the reduced material. The reactant containing a certain substance whose valence is increased is a reducing agent.
In this example, potassium chloride water was electrochemically reacted by 0.15 volts. The solution of the potassium chloride water is subjected to electrochemical reaction by adopting 0.15V voltage, so that the required nano-porous material is uniform in pore size, and the pore size is 60-80nm, so that the subsequent observation by an electron microscope is facilitated.
In this embodiment, the diameter of each pore size in the nanoporous silver structure 53 is 60-80nm. The pore diameter of 60-80nm is adopted, so that each pore diameter in the nano porous silver structure 53 is within the size range, the precise control of the nano porous silver structure 53 is realized, and the subsequent surface modification and the observation of an electron microscope are facilitated.
In this embodiment, the detecting chip 1 includes three detecting chips, which are respectively a first detecting chip 11, a second detecting chip 12 and a third detecting chip 13; the SERS substrate in the first detection chip 11 adopts a nano porous silver structure 51 with a surface modified by a single lipid film; the SERS substrate in the second detection chip adopts a nano porous silver structure 52 with a surface modified by double lipid films; the SERS substrate in the third detection chip adopts a silver sheet nano-porous silver structure 53.
In this embodiment, three detection chips 1 for detecting different molecules to be detected in a sample solution to be detected are provided, which are a first detection chip 11, a second detection chip 12 and a third detection chip 13, wherein the SERS substrate of the first detection chip 11 employs a nano-porous silver structure 51 with a single-layer lipid film modified surface for adsorbing lipophilic pesticides in the molecules to be detected, and a hand-held raman spectrometer is used for detecting raman signals of the molecules to be detected of the nano-porous silver structure 51 with the single-layer lipid film modified surface on the first detection chip 11, and when the molecules to be detected with corresponding properties are adsorbed, if lipophilic pesticides are adsorbed in the first detection chip 5 with the nano-porous silver structure 51 with the single-layer lipid film modified surface, the raman signals are amplified to signals which are six times of ten times of the original raman signals, indicating that the nano-porous silver structure 51 with the single-layer lipid film modified surface adsorbs the lipophilic pesticides, the sample solution to be detected has the lipophilic pesticides; if the raman signal of the molecule to be detected of the nano-porous silver structure 51 with the surface modified by the monolayer lipid film on the first detection chip 11 is detected by the handheld raman spectrometer, no amplification signal is found in the raman signal, that is, the raman signal is not detected or is very weak, it indicates that the nano-porous silver structure 51 with the surface modified by the monolayer lipid film does not adsorb the lipophilic pesticide, that is, the lipophilic pesticide does not exist in the sample solution to be detected or the concentration of the lipophilic pesticide is low enough to be calculated. The SERS substrate of the second detection chip 12 adopts a nanoporous silver structure 52 with a surface modified by a bilayer lipid film, and is used for adsorbing whether the charged pesticide exists in the molecule to be detected; if the raman signal of the molecule to be detected of the nanoporous silver structure 52 with the surface modified by the bilayer lipid membrane on the second detection chip 12 is detected by the handheld raman spectrometer, when the molecule to be detected with the corresponding property is adsorbed, if the charged pesticide is adsorbed in the second detection chip 12 with the nanoporous silver structure 52 with the surface modified by the bilayer lipid membrane, the raman signal is amplified to a signal which is six times of ten times of the original raman signal, which indicates that the nanoporous silver structure 52 with the surface modified by the bilayer lipid membrane adsorbs the charged pesticide, and indicates that the charged pesticide exists in the sample solution to be detected; if the raman signal of the molecule to be detected of the nanoporous silver structure 52 with the surface modified by the bilayer lipid film on the second detection chip 12 is detected by the handheld raman spectrometer, no amplification signal is found in the raman signal, that is, the raman signal is not detected or is very weak, it indicates that the nanoporous silver structure 52 with the surface modified by the bilayer lipid film does not adsorb the charged pesticide, that is, the charged pesticide does not exist in the sample solution to be detected or the concentration of the charged pesticide is low enough to be calculated. The SERS substrate in the third detection chip 13 adopts the nanoporous silver structure 53 for adsorbing the hydrophilic pesticide in the molecule to be detected, the nanoporous silver structure 53 adopts the original nanoporous silver structure 53 without modifying the surface, if the raman signal of the molecule to be detected of the nanoporous silver structure 53 on the third detection chip 13 is detected by the handheld raman spectrometer, when the molecule to be detected with the corresponding property is adsorbed, if the hydrophilic pesticide is adsorbed in the third detection chip 13 where the nanoporous silver structure 53 is located, the raman signal is amplified to a signal which is a sixth power of ten times of the original raman signal, it is indicated that the nanoporous silver structure 53 adsorbs the hydrophilic pesticide, that is, the hydrophilic pesticide exists in the sample solution to be detected; if the raman signal of the molecule to be detected of the nanoporous silver structure 53 on the third detection chip 13 is detected by the handheld raman spectrometer, and the raman signal is not found to have any amplification signal, that is, the raman signal is not detected or is very weak, it indicates that the nanoporous silver structure 53 does not adsorb the hydrophilic pesticide, that is, the sample solution to be detected does not have the hydrophilic pesticide or the concentration of the hydrophilic pesticide is low enough to be calculated. Through setting up multiple passageway, be applied to the detection chip 1 of different SRS bases to detect the molecule of waiting to detect in waiting to detect sample solution of different properties, improve detection efficiency.
In this example, a monolayer lipid film was deposited on the nanoporous silver structure 5 by a technique of constructing an ordered ultrathin molecular film (Langmuir-Blodgett) to obtain a nanoporous silver structure 51 with a monolayer lipid film modification occurring on the surface. More specifically, a unilamellar lipid membrane is deposited on the nanoporous silver sheet by the langmuir-blodgett method, and the surface pressure of the unilamellar lipid membrane can be measured by a williams paper pair pressure sensor to obtain a desired unilamellar lipid membrane.
In this embodiment, the step of surface modification of the nanoporous silver structure to generate monolayer lipid membrane modification comprises:
dropping a certain amount of lipid solution on the water surface through a Hamilton micro-injector until the lipid solution is evaporated to form a lipid film;
extruding the lipid membrane at a preset speed until the lipid membrane bears the target surface pressure;
and (3) pulling up the glass slide positioned in the water and the nano porous silver structure positioned on the glass slide at a specified speed until the nano porous silver structure is exposed out of the water surface, thus obtaining the nano porous silver structure 51 with the surface modified by the monolayer lipid film.
In this example, the step of modifying the surface of the nanoporous silver structure with a monolayer lipid film is as follows: firstly, a Hamilton micro-injector is adopted to drop the lipid solution on the water surface, the amount of the lipid solution dropped on the water surface can be selected according to actual needs, such as the height of 1 mm of the lipid solution paved on the water surface, and can also be adjusted according to the actual needs, after the lipid solution is evaporated for a preset time to form a lipid film, for example, in a specific embodiment, the lipid solution is evaporated for 15 minutes to form a required lipid film, and the lipid film is extruded at a constant speed of 5 mm per minute, so that the lipid film bears the target surface pressure. The nano-porous silver sheet is attached to the glass slide and clamped to the clamp, and the clamp is used for fixing the nano-porous silver sheet on the glass slide. And then, pulling the glass slide upwards at a constant rate of 0.1 millimeter per minute until the silver sheet is completely exposed out of the water surface to realize monolayer lipid membrane modification, wherein the surface pressure of the nano-porous silver sheet on the glass slide is kept unchanged in the process of carrying out the monolayer lipid modification. In other embodiments, the slide in water and the nanoporous silver structure on the slide can be pulled up at other specified speeds until the nanoporous silver structure is exposed to the water. In this example, the illustrated water represents deionized water.
In this embodiment, the step of surface modification of the nanoporous silver structure to generate monolayer lipid membrane modification comprises:
dripping a certain amount of lipid solution on the water surface through a Hamilton micro-injector until the lipid solution is evaporated to form a lipid film;
extruding the lipid membrane at a preset speed until the lipid membrane bears a target surface pressure;
pulling up the glass slide positioned in the water and the nano porous silver structure positioned on the glass slide at a specified speed until the nano porous silver structure is exposed out of the water surface;
the nanoporous silver structure is re-inserted into the water to achieve a surface bilayer lipid membrane modified nanoporous silver structure 52.
In this example, the step of modifying the surface of the nanoporous silver structure with a monolayer lipid film is as follows: firstly, a Hamilton micro-injector is adopted to drop the lipid solution on the water surface, the amount of the lipid solution dropped on the water surface can be selected according to actual needs, such as the height of being flatly paved on the water surface by 1 mm, and can also be adjusted according to the actual needs, after the lipid solution is evaporated for a preset time to form a lipid film, for example, in a specific embodiment, the lipid solution is evaporated for 15 minutes to form a required lipid film, and the lipid film is extruded at a constant speed of 5 mm per minute, so that the lipid film bears the target surface pressure. The nano-porous silver sheet is attached to the glass slide and clamped to the clamp, and the clamp is used for fixing the nano-porous silver sheet on the glass slide. And then, pulling the glass slide upwards at a constant rate of 0.1 millimeter per minute until the silver sheet is completely exposed out of the water surface to realize monolayer lipid membrane modification, wherein the surface pressure of the nano-porous silver sheet on the glass slide is kept unchanged in the process of carrying out the monolayer lipid modification. In other embodiments, the slide in water and the nanoporous silver structure on the slide can be pulled up at other specified speeds until the nanoporous silver structure is exposed to the water. And then the nano porous silver structure is inserted into water again, a lipid film is arranged on the water surface by adopting the mode, and the glass slide is pulled upwards at a constant speed of 0.1 millimeter per minute until the silver sheet is completely exposed out of the water surface to carry out double-layer lipid film modification so as to realize the nano porous silver structure with the surface subjected to double-layer lipid film modification, namely the nano porous silver structure 52 with double-layer surface modification. In this example, the illustrated water represents deionized water.
The double-layer surface modification of the nano-porous silver structure is based on single-layer surface modification, and single-layer surface modification is performed again to obtain the double-layer surface modified nano-porous silver structure 52.
In this example, the reducing agent is sodium borohydride solution. The sodium borohydride solution can be prepared by 0.1M, and the silver sheet plated with silver chloride can be effectively reduced into the nano-porous silver sheet by 0.1 mol of the sodium borohydride solution.
In this embodiment, the input pipeline end of the microfluidic pipeline 2 is set to be a Y-shaped pipeline, the chamber 3 in which the sample solution to be detected is located is communicated with the first port of the Y-shaped pipeline, and the second port of the Y-shaped pipeline is communicated with the chamber 4 in which the cleaning solution for cleaning the microfluidic pipeline 2 is located.
In this embodiment, the input pipeline end of the micro-fluidic pipeline 2 is set to be a Y-shaped pipeline, the first port of the Y-shaped pipeline is communicated with the containing cavity 3 where the sample solution to be detected is located, the second port of the Y-shaped pipeline is communicated with the containing cavity 4 where the cleaning solution is located, a flow direction valve is arranged at the intersection of the Y-shaped pipeline and is used for communicating the output pipeline end of the micro-fluidic pipeline 2 with the sample solution to be detected, or the output pipeline end of the micro-fluidic pipeline 2 is communicated with the cleaning solution, so that the micro-fluidic pipeline can be effectively cleaned, and the reuse is realized.
To sum up, for the detection device who is applied to multichannel SERS micro-fluidic chip that provides in this application embodiment, through setting up a plurality of detection chip 1 that connect in parallel, and set up the nano-porous silver structure 5 that is used for detecting the molecule that detects that corresponds nature on detection chip 1 to solve the thing that detects of different nature and be difficult to detect simultaneously, detection efficiency hangs down the problem, realize multichannel simultaneous detection, improve detection efficiency.
It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, apparatus, article, or method that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, apparatus, article, or method. Without further limitation, an element defined by the phrase "comprising one of 8230, and" comprising 8230does not exclude the presence of additional like elements in a process, apparatus, article, or method comprising the element.
The above description is only for the preferred embodiment of the present application and not intended to limit the scope of the present application, and all modifications of equivalent structures and equivalent processes, which are made by the contents of the specification and the drawings of the present application, or which are directly or indirectly applied to other related technical fields, are intended to be included within the scope of the present application.
Claims (9)
1. The detection device applied to the multichannel SERS microfluidic chip is characterized by comprising a microfluidic pipeline and a detection chip;
the detection chips comprise a plurality of detection chips, the detection chips are connected to the output pipeline end of the microfluidic pipeline in parallel, and the input pipeline end of the microfluidic pipeline is used for inputting a sample solution to be detected;
each detection chip comprises an SERS substrate applied to detection of objects to be detected with different properties, the SERS substrate modifies a nano-silver structure and is used for enriching samples to be detected with different properties and realizing detection, and the SERS substrate comprises any one of the following nano-porous silver structures: the surface of the nano porous silver structure is modified by a monolayer lipid film, the surface of the nano porous silver structure is modified by a double-layer lipid film, the nano porous silver structure modified by the monolayer lipid film is used for adsorbing and detecting lipophilic pesticides, and the nano porous silver structure modified by the double-layer lipid film is used for adsorbing and detecting charged pesticides;
the SERS substrate adsorbs molecules to be detected in the sample solution to be detected, and a Raman signal of the molecules to be detected adsorbed by the detection chip is detected through a handheld Raman spectrometer;
the detection chips comprise three detection chips, namely a first detection chip, a second detection chip and a third detection chip;
the SERS substrate in the first detection chip adopts a nano porous silver structure with a surface modified by a single lipid film;
the SERS substrate in the second detection chip adopts a nano porous silver structure with the surface modified by double lipid films;
and the SERS substrate in the third detection chip adopts the nano porous silver structure.
2. The detecting device applied to the multi-channel SERS microfluidic chip as recited in claim 1, wherein the manufacturing process of the SERS substrate comprises:
washing the silver flakes with the purity of 99.9% by using double deionized water, and placing the washed silver flakes into potassium chloride water with preset concentration to perform electrochemical reaction to obtain silver flakes plated with silver chloride;
stirring the solution in which the silver pieces plated with the silver chloride are positioned, placing the silver pieces plated with the silver chloride into a reducing agent, and reducing the silver pieces plated with the silver chloride into nano-porous silver pieces in situ through the reducing agent;
and washing the porous silver sheet by using deionized water and ethanol to obtain the nano porous silver structure.
3. The detecting device applied to the multi-channel SERS microfluidic chip as recited in claim 2, wherein the electrochemical reaction is performed on potassium chloride water by 0.15V.
4. The detecting device applied to the multichannel SERS microfluidic chip according to any one of claims 1 to 3, wherein the diameter of each pore in the nanoporous silver structure is 60-80nm.
5. The detection device applied to the multichannel SERS microfluidic chip as recited in any one of claims 1 to 4, wherein a single lipid film is deposited on the nanoporous silver structure by a technology of constructing an ordered ultrathin molecular film, so as to obtain the nanoporous silver structure with the surface modified by the single lipid film.
6. The detection device applied to the multi-channel SERS microfluidic chip as recited in claim 5, wherein the step of modifying the surface of the nanoporous silver structure by a single lipid film comprises:
dropping a certain amount of lipid solution on the water surface through a Hamilton micro-injector until the lipid solution is evaporated to form a lipid membrane;
pressing the lipid membrane at a preset speed until the lipid membrane is subjected to a target surface pressure;
and (3) pulling up the glass slide positioned in the water and the nano porous silver structure positioned on the glass slide at a specified speed until the nano porous silver structure is exposed out of the water surface, thus obtaining the nano porous silver structure with the surface modified by the monolayer lipid film.
7. The detection device applied to the multi-channel SERS microfluidic chip as recited in claim 5, wherein the step of modifying the surface of the nanoporous silver structure by a single lipid film comprises:
dropping a certain amount of lipid solution on the water surface through a Hamilton micro-injector until the lipid solution is evaporated to form a lipid film;
pressing the lipid membrane at a preset speed until the lipid membrane is subjected to a target surface pressure;
pulling up the glass slide positioned in the water and the nano porous silver structure positioned on the glass slide at a designated speed until the nano porous silver structure is exposed out of the water surface;
and inserting the nano-porous silver structure into the water again to realize the nano-porous silver structure with the surface modified by the double lipid films.
8. The detecting device applied to the multi-channel SERS microfluidic chip according to claim 2, wherein the reducing agent is a sodium borohydride solution.
9. The detecting device applied to the multi-channel SERS microfluidic chip according to claim 1, wherein an input pipeline end of the microfluidic pipeline is set to be a Y-shaped pipeline, a containing cavity where the sample solution to be detected is located is communicated with a first port of the Y-shaped pipeline, and a second port of the Y-shaped pipeline is communicated with a containing cavity where a cleaning solution for cleaning the microfluidic pipeline is located.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210862608.XA CN115096871B (en) | 2022-07-22 | 2022-07-22 | Detection device applied to multichannel SERS micro-fluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210862608.XA CN115096871B (en) | 2022-07-22 | 2022-07-22 | Detection device applied to multichannel SERS micro-fluidic chip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115096871A CN115096871A (en) | 2022-09-23 |
| CN115096871B true CN115096871B (en) | 2022-12-23 |
Family
ID=83298863
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210862608.XA Active CN115096871B (en) | 2022-07-22 | 2022-07-22 | Detection device applied to multichannel SERS micro-fluidic chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115096871B (en) |
Citations (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6149868A (en) * | 1997-10-28 | 2000-11-21 | The Penn State Research Foundation | Surface enhanced raman scattering from metal nanoparticle-analyte-noble metal substrate sandwiches |
| WO2001007892A1 (en) * | 1999-07-27 | 2001-02-01 | Esperion Therapeutics, Inc. | Method and device for measurement of cholesterol efflux |
| JP2005140794A (en) * | 2005-01-13 | 2005-06-02 | E Graw An | Raman opto load process and device for detecting chemical substance and microorganism |
| WO2006048660A1 (en) * | 2004-11-04 | 2006-05-11 | Mesophotonics Limited | Metal nano-void photonic crystal for enhanced raman spectroscopy |
| WO2009020479A2 (en) * | 2007-04-27 | 2009-02-12 | The Regents Of The University Of California | Device and methods for detection of airborne agents |
| KR20090015779A (en) * | 2007-08-09 | 2009-02-12 | 재단법인서울대학교산학협력재단 | A method for adsorption using solid thin film mask of nano-particle and adsorption matter |
| CA2667040A1 (en) * | 2008-06-09 | 2009-12-09 | The University Of Georgia Research Foundation, Inc. | Surface enhanced raman spectroscopy (sers) systems for the detection of viruses and methods of use thereof |
| WO2012094727A1 (en) * | 2010-10-29 | 2012-07-19 | The Governing Council Of The University Of Toronto | Lipid encapsulation of surface enhanced raman scattering (sers) nanoparticles |
| CN102608311A (en) * | 2012-04-19 | 2012-07-25 | 湖南大学 | Biological sensing method for cholera toxin based on agglomeration of phospholipid bilayer membrane covered nanoparticles |
| CN102680452A (en) * | 2012-05-14 | 2012-09-19 | 中央民族大学 | Dual-detection biochemical sensing detector integrated with optofluidics |
| WO2013070948A1 (en) * | 2011-11-09 | 2013-05-16 | The Regents Of The University Of Michigan | Sers, fluorescence, absorption, and luminescence detection with flow-through multi-hole capillaries |
| CN103196887A (en) * | 2013-03-27 | 2013-07-10 | 重庆绿色智能技术研究院 | High-throughput microfluidic device for organic pesticide detection, and water sample detection method of same |
| CN103439309A (en) * | 2013-08-19 | 2013-12-11 | 南通大学 | Preparation method for high-sensitivity CO gas spectrum sensor |
| JP2014021025A (en) * | 2012-07-20 | 2014-02-03 | Hiroshi Sotooka | Apparatus and method for forming artificial lipid membrane |
| CN104132921A (en) * | 2014-07-07 | 2014-11-05 | 华南师范大学 | Chemical vapor deposition based method for preparing surface Raman enhanced active substrate |
| CN104568905A (en) * | 2015-01-19 | 2015-04-29 | 东南大学 | Three-dimensional code biological detection chip based on surface-enhanced Raman scattering (SERS) microflow platform as well as preparation method and detection method of biological detection chip |
| CN105784667A (en) * | 2014-12-22 | 2016-07-20 | 深圳先进技术研究院 | Surface enhanced Raman scattering substrate, preparation method and applications thereof |
| CN106281288A (en) * | 2016-08-04 | 2017-01-04 | 陕西森瑞石油技术开发有限公司 | A kind of low permeability reservoir pressure reducing and injection increasing agent used for flooding well and preparation method thereof |
| CN106669872A (en) * | 2016-12-29 | 2017-05-17 | 中国人民解放军国防科学技术大学 | Surface-enhanced Raman microfluidic test chip as well as preparation method and application thereof |
| CA2949634A1 (en) * | 2016-11-25 | 2018-05-25 | Medmira Inc. | Analyte detection using raman spectroscopy |
| CN108709880A (en) * | 2018-05-28 | 2018-10-26 | 中国工程物理研究院化工材料研究所 | Reusable high throughput SERS micro-fluidic chips and its application |
| CN109142313A (en) * | 2018-08-03 | 2019-01-04 | 吉林大学 | The diamond substrate and preparation method thereof of semiconductor surface enhancing Raman scattering |
| CN109358033A (en) * | 2019-01-08 | 2019-02-19 | 中国科学院烟台海岸带研究所 | One seed nucleus-satellite type gold and silver composite Nano SERS substrate and preparation method thereof |
| US10526441B1 (en) * | 2019-01-23 | 2020-01-07 | King Fahd University Of Petroleum And Minerals | Polypyrrole-coated silver particles for surface enhanced Raman scattering |
| CN111175274A (en) * | 2019-12-31 | 2020-05-19 | 浙江大学 | Application of PDMS film in SERS high-sensitivity detection |
| CN112014373A (en) * | 2020-08-28 | 2020-12-01 | 陕西理工大学 | SERS Raman substrate, preparation method thereof and method for analyzing and detecting pesticide residues |
| EP3811078A1 (en) * | 2018-06-11 | 2021-04-28 | Dublin City University | A method and device for assaying the interaction and dynamics of permeation of a molecule and a lipid bilayer |
| EP3849705A1 (en) * | 2018-09-14 | 2021-07-21 | Berkeley Lights, Inc. | Methods for assaying binding affinity |
| CN113433111A (en) * | 2021-06-25 | 2021-09-24 | 辽宁大学 | Liposome-based core-shell material for Raman spectrum detection and preparation method and application thereof |
| CN113624744A (en) * | 2021-09-08 | 2021-11-09 | 首都师范大学 | Microfluidic in-situ SERS detection method for detecting trace mercury ions |
| CN114577779A (en) * | 2022-03-10 | 2022-06-03 | 丹娜(天津)生物科技股份有限公司 | Micro-fluidic drug sensitive plate for rapidly detecting microbial drug sensitivity based on Raman spectrum and application thereof |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7271896B2 (en) * | 2003-12-29 | 2007-09-18 | Intel Corporation | Detection of biomolecules using porous biosensors and raman spectroscopy |
| US7776547B2 (en) * | 2004-08-26 | 2010-08-17 | Intel Corporation | Cellular analysis using Raman surface scanning |
| US7659977B2 (en) * | 2006-04-21 | 2010-02-09 | Intel Corporation | Apparatus and method for imaging with surface enhanced coherent anti-stokes raman scattering (SECARS) |
| CN102023150B (en) * | 2009-09-15 | 2012-10-10 | 清华大学 | Raman scattering substrate and detection system with same |
| EP2619532A1 (en) * | 2010-09-20 | 2013-07-31 | Vanderbilt University | Nanoscale porous gold film sers template |
| US9019495B2 (en) * | 2012-07-31 | 2015-04-28 | Hewlett-Packard Development Company, L.P. | Apparatus for performing spectroscopy having a porous membrane |
| US20150185156A1 (en) * | 2012-07-31 | 2015-07-02 | Northwestern University | Dispersible Surface-Enhanced Raman Scattering Nanosheets |
| US20140106469A1 (en) * | 2012-10-11 | 2014-04-17 | The Regents Of The University Of California | Nanoparticle Plasmonic Sensor for Localized Surface Plasmon Resonance |
| CN103341170B (en) * | 2013-07-08 | 2015-03-25 | 东南大学 | Dual-controllable medicine release structure with SERS (Surface Enhanced Raman Scattering) signal and preparation method of dual-controllable medicine release structure |
| US9804093B2 (en) * | 2014-05-23 | 2017-10-31 | University Of Notre Dame Du Lac | Ultrasensitive SERS flow detector |
| CA2913428A1 (en) * | 2014-12-03 | 2016-06-03 | Bubble Technology Industries Inc. | System and method for detection of contaminants |
| CN105717221A (en) * | 2016-01-30 | 2016-06-29 | 崔淑华 | GC-EI-MS rapid measurement method for penflufen residual amount |
| CN114594247A (en) * | 2022-03-22 | 2022-06-07 | 中国计量大学 | Microfluidic SERS chip manufacturing and detecting method based on immunoassay |
-
2022
- 2022-07-22 CN CN202210862608.XA patent/CN115096871B/en active Active
Patent Citations (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6149868A (en) * | 1997-10-28 | 2000-11-21 | The Penn State Research Foundation | Surface enhanced raman scattering from metal nanoparticle-analyte-noble metal substrate sandwiches |
| WO2001007892A1 (en) * | 1999-07-27 | 2001-02-01 | Esperion Therapeutics, Inc. | Method and device for measurement of cholesterol efflux |
| WO2006048660A1 (en) * | 2004-11-04 | 2006-05-11 | Mesophotonics Limited | Metal nano-void photonic crystal for enhanced raman spectroscopy |
| JP2005140794A (en) * | 2005-01-13 | 2005-06-02 | E Graw An | Raman opto load process and device for detecting chemical substance and microorganism |
| WO2009020479A2 (en) * | 2007-04-27 | 2009-02-12 | The Regents Of The University Of California | Device and methods for detection of airborne agents |
| KR20090015779A (en) * | 2007-08-09 | 2009-02-12 | 재단법인서울대학교산학협력재단 | A method for adsorption using solid thin film mask of nano-particle and adsorption matter |
| CA2667040A1 (en) * | 2008-06-09 | 2009-12-09 | The University Of Georgia Research Foundation, Inc. | Surface enhanced raman spectroscopy (sers) systems for the detection of viruses and methods of use thereof |
| WO2012094727A1 (en) * | 2010-10-29 | 2012-07-19 | The Governing Council Of The University Of Toronto | Lipid encapsulation of surface enhanced raman scattering (sers) nanoparticles |
| WO2013070948A1 (en) * | 2011-11-09 | 2013-05-16 | The Regents Of The University Of Michigan | Sers, fluorescence, absorption, and luminescence detection with flow-through multi-hole capillaries |
| CN102608311A (en) * | 2012-04-19 | 2012-07-25 | 湖南大学 | Biological sensing method for cholera toxin based on agglomeration of phospholipid bilayer membrane covered nanoparticles |
| CN102680452A (en) * | 2012-05-14 | 2012-09-19 | 中央民族大学 | Dual-detection biochemical sensing detector integrated with optofluidics |
| JP2014021025A (en) * | 2012-07-20 | 2014-02-03 | Hiroshi Sotooka | Apparatus and method for forming artificial lipid membrane |
| CN103196887A (en) * | 2013-03-27 | 2013-07-10 | 重庆绿色智能技术研究院 | High-throughput microfluidic device for organic pesticide detection, and water sample detection method of same |
| CN103439309A (en) * | 2013-08-19 | 2013-12-11 | 南通大学 | Preparation method for high-sensitivity CO gas spectrum sensor |
| CN104132921A (en) * | 2014-07-07 | 2014-11-05 | 华南师范大学 | Chemical vapor deposition based method for preparing surface Raman enhanced active substrate |
| CN105784667A (en) * | 2014-12-22 | 2016-07-20 | 深圳先进技术研究院 | Surface enhanced Raman scattering substrate, preparation method and applications thereof |
| CN104568905A (en) * | 2015-01-19 | 2015-04-29 | 东南大学 | Three-dimensional code biological detection chip based on surface-enhanced Raman scattering (SERS) microflow platform as well as preparation method and detection method of biological detection chip |
| CN106281288A (en) * | 2016-08-04 | 2017-01-04 | 陕西森瑞石油技术开发有限公司 | A kind of low permeability reservoir pressure reducing and injection increasing agent used for flooding well and preparation method thereof |
| CA2949634A1 (en) * | 2016-11-25 | 2018-05-25 | Medmira Inc. | Analyte detection using raman spectroscopy |
| WO2018094528A1 (en) * | 2016-11-25 | 2018-05-31 | Medmira Inc. | Analyte detection using raman spectroscopy |
| CN106669872A (en) * | 2016-12-29 | 2017-05-17 | 中国人民解放军国防科学技术大学 | Surface-enhanced Raman microfluidic test chip as well as preparation method and application thereof |
| CN108709880A (en) * | 2018-05-28 | 2018-10-26 | 中国工程物理研究院化工材料研究所 | Reusable high throughput SERS micro-fluidic chips and its application |
| EP3811078A1 (en) * | 2018-06-11 | 2021-04-28 | Dublin City University | A method and device for assaying the interaction and dynamics of permeation of a molecule and a lipid bilayer |
| CN109142313A (en) * | 2018-08-03 | 2019-01-04 | 吉林大学 | The diamond substrate and preparation method thereof of semiconductor surface enhancing Raman scattering |
| EP3849705A1 (en) * | 2018-09-14 | 2021-07-21 | Berkeley Lights, Inc. | Methods for assaying binding affinity |
| CN109358033A (en) * | 2019-01-08 | 2019-02-19 | 中国科学院烟台海岸带研究所 | One seed nucleus-satellite type gold and silver composite Nano SERS substrate and preparation method thereof |
| US10526441B1 (en) * | 2019-01-23 | 2020-01-07 | King Fahd University Of Petroleum And Minerals | Polypyrrole-coated silver particles for surface enhanced Raman scattering |
| CN111175274A (en) * | 2019-12-31 | 2020-05-19 | 浙江大学 | Application of PDMS film in SERS high-sensitivity detection |
| CN112014373A (en) * | 2020-08-28 | 2020-12-01 | 陕西理工大学 | SERS Raman substrate, preparation method thereof and method for analyzing and detecting pesticide residues |
| CN113433111A (en) * | 2021-06-25 | 2021-09-24 | 辽宁大学 | Liposome-based core-shell material for Raman spectrum detection and preparation method and application thereof |
| CN113624744A (en) * | 2021-09-08 | 2021-11-09 | 首都师范大学 | Microfluidic in-situ SERS detection method for detecting trace mercury ions |
| CN114577779A (en) * | 2022-03-10 | 2022-06-03 | 丹娜(天津)生物科技股份有限公司 | Micro-fluidic drug sensitive plate for rapidly detecting microbial drug sensitivity based on Raman spectrum and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115096871A (en) | 2022-09-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Weng et al. | Recent advances in thread-based microfluidics for diagnostic applications | |
| Rattanarat et al. | Graphene-polyaniline modified electrochemical droplet-based microfluidic sensor for high-throughput determination of 4-aminophenol | |
| Tang et al. | Bacteria detection based on its blockage effect on silicon nanopore array | |
| Shariati et al. | An ultrasensitive label free human papilloma virus DNA biosensor using gold nanotubes based on nanoporous polycarbonate in electrical alignment | |
| Gao et al. | Emerging paper microfluidic devices | |
| CN105772118B (en) | The SERS micro-fluidic chips and preparation method of integrated enhancing substrate on ITO electro-conductive glass | |
| EP1490508B1 (en) | Electrochemical detector systems | |
| EP3247998B1 (en) | Microfluidic particle analysis device | |
| EP2294410A1 (en) | Conductivity sensor device comprising diamond film with at least one nanopore or micropore | |
| Arshad et al. | Recent developments and applications of nanomaterial-based lab-on-a-chip devices for sustainable agri-food industries | |
| Ghrera et al. | Quantum dot-based microfluidic biosensor for cancer detection | |
| CA2920584A1 (en) | Microchip structure and treatments for electrochemical detection | |
| EP2084532A1 (en) | Porous biological assay substrate and method and device for producing such substrate | |
| Tran et al. | A state-of-the-art review on graphene-based nanomaterials to determine antibiotics by electrochemical techniques | |
| Tang et al. | Label-free potentiometric aptasensing platform for the detection of Pb2+ based on guanine quadruplex structure | |
| Karthikeyan et al. | Fluorometric sensor for mercury ion detection in a fluidic MEMS device | |
| Abhilasha et al. | The Model of Smart Sensing Device For Sensitive Nanoclusters Modification in Sensing Properties | |
| Bilican et al. | Alternative screening method for analyzing the water samples through an electrical microfluidics chip with classical microbiological assay comparison of P. aeruginosa | |
| Zhang et al. | The recent development of nanomaterials enhanced paper-based electrochemical analytical devices | |
| Rasheed et al. | Dual-mode highly selective colorimetric and smartphone-based paper sensors utilizing silver nanoparticles for ultra-trace level omeprazole detection in complex matrices | |
| DE19710525A1 (en) | Device and method for sampling in fluid phases with a diffusion body and an analyte-binding further phase | |
| CN115096871B (en) | Detection device applied to multichannel SERS micro-fluidic chip | |
| Srinivasaraghavan et al. | A comparative study of nano-scale coatings on gold electrodes for bioimpedance studies of breast cancer cells | |
| Chen et al. | Modification-free pumpless laser-induced graphene multichannel open microfluidic electrode chip for simultaneous quantification of dual targets | |
| Chen et al. | Multi-dimensional microfluidic paper-based analytical devices (μPADs) for noninvasive testing: A review of structural design and applications |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhu Hongni Inventor after: Huang Jinqing Inventor before: Zhu Hongni |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |