CN115089891B - Device for stimulating caudate nucleus in brain to improve nervous system excitability - Google Patents

Abstract

The present invention provides a device for stimulating the caudate nucleus in the brain to increase nervous system excitability, comprising: the infrared spot frequency light source module is used for generating high-power infrared light with the wavelength of 8.5 microns and the luminous power of 35 mW; the optical fiber coupling module is used for reducing the infrared light and carrying out focused beam processing on the infrared light based on the multi-stage focusing component to obtain focused infrared light, and the focused infrared light is collimated by the collimating component and then coupled into the optical fiber for conduction; the puncture guiding component is used for guiding the front end of the optical fiber to a specific irradiation stimulation site in the brain and irradiating the infrared light coupled into the optical fiber on the caudate nucleus in the brain. The device for stimulating the caudate nucleus in the brain to improve the excitability of the nervous system can reduce nervous system inhibition and fatigue feeling caused by excessive secretion of gamma-aminobutyric acid in the brain by stimulating the caudate nucleus in the brain through infrared light, indirectly control the excitability degree of the nervous system of an organism, and has the advantages of simple operation, safety, reliability, rapid regulation and control and short time.

Description

A device used to stimulate the caudate nucleus in the brain to increase the excitability of the nervous system

technical field

The invention relates to the technical field of biological effects of infrared light, in particular to a device for stimulating the caudate nucleus in the brain to improve the excitability of the nervous system.

Background technique

As the most important inhibitory neurotransmitter widely distributed in the brain, γ-aminobutyric acid plays an important role in maintaining normal physiological functions of the brain. However, excessive secretion of gamma-aminobutyric acid will reduce the activity of nerve cells, inhibit the excitability of the central nervous system, cause people to experience drowsiness, sluggishness, lethargy and other adverse symptoms, and affect the body's exercise level. Previous studies have found that long-term exercise can weaken the metabolic process of GABA and increase the content of GABA in brain tissue, thereby inhibiting central nervous cells and causing fatigue. Therefore, reducing the γ-aminobutyric acid in the brain can improve the excitability of the nervous system and reduce the fatigue symptoms of the nervous system. At present, most of the traditional methods for regulating the change of GABA content in the brain are drugs, which not only have contraindications to medication, but also have side effects, and the onset time is slow. Therefore, how to design an external stimulation device that can efficiently and quickly solve the problems of central nervous system excitatory inhibition caused by excessive γ-aminobutyric acid secretion has become an urgent problem to be solved.

Contents of the invention

Therefore, the present invention provides a device for stimulating the caudate nucleus in the brain to increase the excitability of the nervous system, so as to solve the problems of high limitations and poor reliability of the existing drug regulation methods.

The invention provides a device for stimulating the caudate nucleus in the brain to improve the excitability of the nervous system, comprising:

Infrared point-frequency light source module, used to generate high-power infrared light with a wavelength of 8.5 μm and an optical power of less than or equal to 200 mW;

The optical fiber coupling module shrinks and focuses the infrared light based on a multi-stage focusing component to obtain focused infrared light, and then collimates the focused infrared light through a collimating component and then couples it into an optical fiber for transmission;

The puncture guide part is used to guide the front end of the optical fiber to a specific irradiation stimulation site in the brain, and irradiate the infrared light coupled into the optical fiber on the caudate nucleus of the brain; wherein, the irradiation stimulation site for the caudate nucleus.

Further, the puncture guiding component includes: an inner guide wire, an outer sheath, and a fixed switch;

The inner guide wire is used to puncture into the irradiation stimulation site in the brain; the end of the inner guide wire is provided with a buckle corresponding to the slot of the outer sheath, so that the inner guide wire capable of snap-fitting with and removably withdrawn from the sheath;

The outer sheath is used to puncture and place the irradiation stimulation site under the guidance of the inner guide wire, and guide the optical fiber to be inserted into the irradiation stimulation site along the outer sheath after the inner guide wire is withdrawn from the outer sheath;

The fixed switch is used to fix the optical fiber in the outer sheath by mechanical pressure after being closed, so that the optical fiber is fixedly inserted into a corresponding depth.

Further, after the fixed switch is closed, a tubular channel is formed in the middle for passing and fixing the optical fiber, and the inner surface of the tubular channel is made of soft material to protect the optical fiber from being damaged by mechanical pressure.

Further, the fixed switch is provided with buckles corresponding to the slots on the outer sheath, so as to enable the fixed switch to be engaged with and disassembled from the outer sheath during the process of fixing the optical fiber.

Further, the inner diameter of the outer sheath is 1.1mm, and the outer diameter is 1.3mm; the material hardness of the outer sheath is 70-80HD, the color is transparent, and the surface is marked with scales.

Further, the diameter of the inner guide wire is 0.8-0.9mm; the material hardness of the inner guide wire is 70-80HD.

Further, the fiber coupling module includes: the multi-stage focusing component, the collimating component and the optical fiber;

The multi-stage focusing component is used to reduce the spot of the infrared light emitted by the infrared point-frequency light source module, so that the radius of the spot of the infrared light is reduced to less than or equal to 600 μm, and then the focused infrared light is obtained; The collimation component is used to collimate the focused infrared light so that the light beam is irradiated along a straight line; the optical fiber is used to conduct the collimated infrared light.

Further, the optical fiber is a multimode optical fiber with an inner diameter of 580-610 μm, an outer diameter of 900-1000 μm, and a numerical aperture of 0.3, which is used to effectively transmit a light beam with a wavelength of 8.5 μm, so that the output power of the fiber is 35 mW.

Further, the repetition frequency of the infrared light is less than or equal to 200 kHz, and the pulse width is less than or equal to 2 μs.

Further, the device is set to irradiate the infrared light on the caudate nucleus of the brain for 5 minutes.

The device provided by the present invention for stimulating the caudate nucleus in the brain to improve the excitability of the nervous system generates high-power infrared light with a wavelength of 8.5 μm and an optical power of less than or equal to 200 mW through an infrared point-frequency light source module; Based on the multi-stage focusing components to shrink the infrared light and process the focused beam to obtain the focused infrared light, the focused infrared light is collimated by the collimation component and then coupled into the optical fiber for transmission; and the puncture guide component is used to The infrared light emitted by the front end of the optical fiber with a power of 35mW is guided to a specific irradiation stimulation site in the brain, and the infrared light coupled into the optical fiber is irradiated on the caudate nucleus of the brain, which can stimulate the caudate nucleus of the brain through infrared light to reduce the γ- Nervous system depression and fatigue caused by excessive GABA secretion indirectly control the nervous system excitement of the organism, the operation is simple, safe and reliable, the regulation is rapid and the time is short.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description These are some embodiments of the present invention. For those skilled in the art, other drawings can also be obtained based on these drawings without creative effort.

Fig. 1 is a schematic structural view of a device for stimulating the caudate nucleus in the brain to improve the excitability of the nervous system provided by an embodiment of the present invention;

Fig. 2 is a schematic structural diagram of an optical fiber coupling module provided by an embodiment of the present invention;

Fig. 3 is a schematic structural diagram of a puncture guide part provided by an embodiment of the present invention;

Fig. 4 is a schematic diagram of the physical structure of the device for stimulating the caudate nucleus in the brain to improve the excitability of the nervous system provided by the embodiment of the present invention;

Fig. 5 is a schematic structural view of the outer sheath in the device for stimulating the caudate nucleus in the brain to improve the excitability of the nervous system provided by the embodiment of the present invention;

6 is a schematic structural view of the inner guide wire in the device for stimulating the caudate nucleus in the brain to improve the excitability of the nervous system provided by the embodiment of the present invention;

Fig. 7 is a schematic structural view of a fixed switch in a device for stimulating the caudate nucleus in the brain to improve the excitability of the nervous system provided by an embodiment of the present invention;

Fig. 8 is the γ-aminobutyric acid (GABA) result chart of the test group and the control group under the stimulation of 8.5 μm wavelength provided by the embodiment of the present invention;

Fig. 9 is the result figure of the Western blot test of the neuronal core antigen (NeuN) of the test group and the control group provided by the embodiment of the present invention;

Fig. 10 is an analysis diagram of the results of the Western blot test of neuronal core antigen (NeuN) in the test group and the control group provided by the embodiment of the present invention.

Detailed ways

In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments It is a part of embodiments of the present invention, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

The following is a detailed description of the embodiment of the device for stimulating the caudate nucleus of the brain to improve the excitability of the nervous system according to the present invention. As shown in Figures 1 and 4, it is a structural schematic diagram and a physical structural schematic diagram of a device for stimulating the caudate nucleus in the brain to improve the excitability of the nervous system provided by the embodiment of the present invention. The specific implementation process includes the following parts: Infrared point-frequency light source module (that is, a high-power infrared point-frequency light source 101 ), an optical fiber coupling module 102 and a puncture guiding component 103 .

In the embodiment of the present invention, the infrared point-frequency light source module is used to generate high-power infrared light with a wavelength of 8.5 μm and an optical power less than or equal to 200 mW. The fiber coupling module 102 is used to shrink and focus the infrared light based on the multi-stage focusing component 201 to obtain the focused infrared light, and collimate the focused infrared light through the collimating component 202 coupled into the optical fiber 203 for conduction. The puncture guiding part 103 is used to guide the front end of the optical fiber 203 to a specific irradiation stimulation site in the brain, and irradiate the infrared light coupled into the optical fiber 203 on the caudate nucleus of the brain, thereby protecting the optical fiber The front end of 203 is protected from blood and cerebrospinal fluid of the brain tissue. The irradiation stimulation site is the caudate nucleus.

Further, the infrared light is infrared light with a repetition frequency less than or equal to 200 kHz and a pulse width less than or equal to 2 μs. When the repetition frequency of the infrared light is less than or equal to 200 kHz, and the pulse width is less than or equal to 2 μs, it is beneficial to reduce the infrared photothermal effect while giving high-frequency stimulation to the deep functional areas of the brain.

As shown in FIG. 3 , the puncture guiding component 103 includes: an inner guide wire 301 , an outer sheath 302 and a fixed switch 303 .

The inner guide wire 301 is used to puncture into the irradiation stimulation site in the brain; the end of the inner guide wire 301 is provided with a buckle 602 corresponding to the slot 503 of the outer sheath 302, so that during the puncture The inner guide wire 301 can be fastened with the outer sheath 302 and withdrawn from the outer sheath 302 detachably. Specifically, as shown in FIG. 6 , the inner guide wire 301 has a diameter of 0.8-0.9 mm, a hard plastic material with a hardness of 86-90 HD, and a sharp front end 601 for puncturing into a specific site in the brain. There is a buckle 602 at the end of the inner guide wire 301 corresponding to the slot 503 of the outer sheath 302 to ensure that the inner guide wire 301 is closely combined with the outer sheath 302 during the puncture process so as not to be displaced relative to each other. At the same time, lightly rotate the inner guide wire 301 clockwise to detachably withdraw from the outer sheath 302 .

The outer sheath 302 is used to puncture and place the irradiation stimulation site under the guidance of the inner guide wire 301, and guide the optical fiber 203 to be inserted along the outer sheath 302 after the inner guide wire 301 is withdrawn from the outer sheath 302 Irradiate the site of stimulation. Specifically, as shown in Figure 5, the inner diameter of the outer sheath 302 is 1.1 mm, the outer diameter is 1.3 mm, and the hardness of the material is medium hard plastic of 70-80 HD. The puncture depth can be observed in real time to ensure the accuracy of the puncture), the material is tough and flexible (on the one hand, it can ensure that the insertion angle of the optical fiber 203 is not biased, on the other hand, it can conform to the softness of the brain tissue, and protect the brain tissue to the greatest extent), and the front end is smooth (The smooth front end can ensure that it is placed in the brain for a long time without damaging the brain tissue); the outer sheath 302 is punctured into the stimulation site under the guidance of the inner guide wire 301, and after the outer sheath 302 is fixed, the inner guide wire 301 is withdrawn, and then Optical fiber 203 may be placed along outer sheath 302 at the stimulation site.

The fixing switch 303 is used to fix the optical fiber 203 in the outer sheath 302 by mechanical pressure after being closed, so that the optical fiber 203 is fixedly inserted into a corresponding depth. As shown in FIG. 7 , the fixed switch 303 forms a tubular channel 701 for passing and fixing the optical fiber 203 in the middle after it is closed. The inner surface of the tubular channel is made of a soft material, and the optical fiber can pass through the tubular channel. play a fixed role. The hinge link 703 can be rotated to realize the closing of the fixed switch, and then fix the optical fiber through the mechanical pressure after closing. During the process of fixing the optical fiber 203 , the fixing switch 303 can be fastened with the outer sheath 302 . Specifically, the fixed switch 303 is used to fix the optical fiber 203 on the outer sheath 302 to ensure that the optical fiber 203 is placed at an appropriate depth and will not go too deep, and at the same time ensure that it will not be displaced during the test, causing brain tissue damage and optical fiber damage. 203 itself suffers from stress damage. The fixed switch 303 can be turned on and off repeatedly. After closing, a tubular channel will be formed in the middle (through which the optical fiber 203 passes). The inner surface of the fixed switch 303 is relatively soft (it can adapt to the thickness of the optical fiber 203, and the optical fiber 203 will not be damaged due to mechanical pressure). There is a buckle 702 on the outer surface of the fixed switch 303, which can correspond to the groove 503 of the outer sheath 302 (the groove 503 is also the groove 503 corresponding to the buckle 602 of the inner guide wire 301). Before the puncture operation, the inner guide wire 301 needs to be removed, the fixed switch 303 is mounted on the outer sheath 302 and the switch is kept on. Then, the optical fiber 203 is inserted through the outer sheath 302 , and after checking the insertion depth with the scale on the outer sheath 302 , the fixed switch 303 is closed, and the optical fiber 203 is clamped. Finally, the fixed switch 303 is removed together with the optical fiber 203, and the inner guide wire 301 is reinstalled to start the puncture operation.

As shown in FIG. 2 , the fiber coupling module 102 includes: the collimating component 202 , the multi-stage focusing component 201 and the optical fiber 203 . The multi-stage focusing component 201 is used to reduce the spot of the infrared light emitted by the infrared point-frequency light source module, so that the radius of the spot of the infrared light is reduced to less than or equal to 600 μm, and then the focused infrared light is obtained; The collimation component 202 is used to collimate the focused infrared light so that the light beam is irradiated along a straight line, thereby reducing light scattering; the optical fiber 203 is used to transmit the collimated infrared light. Specifically, the optical fiber 203 is a multimode optical fiber with an inner diameter of 580-610 μm, an outer diameter of 900-1000 μm, and a numerical aperture of 0.3, which can be used to effectively transmit light beams with a wavelength of 8.5 μm, so that the output power of the fiber is 35 mW.

The present invention finds through analysis that the above-mentioned high-power infrared light with a specific wavelength of 8.5 μm and an optical power intensity of 35 mW stimulates the caudate nucleus for 5 minutes, which can reduce the secretion of gamma-aminobutyric acid in the brain. The reduction of the concentration of gamma-aminobutyric acid will increase the excitability of the biological nervous system, so in the actual implementation process, the device is set to irradiate the infrared light on the caudate nucleus of the brain for 5 minutes, so that The device indirectly controls the excitability of the nervous system of the organism and reduces the drowsiness and drowsiness caused by brain fatigue.

In the actual test process, the device was used to carry out relevant tests on 8 SD rats aged 8 weeks. The specific test process is as follows: a total of 8 SD rats at the age of 8 weeks with similar physiological status (such as size, weight, etc.) were randomly selected and divided into 2 groups. There were 4 rats in the control group and the stimulation group respectively. Perform the same anesthesia and craniotomy on each group of rats, then fix the rats on the animal head frame stereotaxic apparatus, then position and puncture the puncture guide part 103 into the caudate nucleus, and guide the optical fiber 203 through the puncture guide part 103 And fixed in the caudate nucleus stimulation point. Finally, adjust the parameters and turn on the high-power point-frequency infrared light source. The stimulation group was irradiated with infrared light with a wavelength of 8.5 μm for 5 min. In the control group, only optical fiber 203 was inserted for 5 minutes without infrared light irradiation. After the end, open the rat skull, take out the rat brain, remove the cerebellum and brainstem, and put it into a cryopreservation tube for storage in liquid nitrogen. The brain samples of each rat were extracted to detect the γ-aminobutyric acid in the rat body, and its concentration was analyzed. Repeat the test 3 times as above, get the mean value of the γ-aminobutyric acid concentration of the 3 tests for statistical analysis, and make the column analysis chart of the γ-aminobutyric acid results in Figure 8 (the abscissa is the classification of the control group and the stimulation group, The vertical axis is the concentration value). The abscissa T is the concentration data of the test group stimulated by infrared light, and the abscissa C is the concentration data of the control group, only the optical fiber is inserted, but no stimulation; the ordinate represents the concentration.

By analyzing the concentration of γ-aminobutyric acid, it can be found that when the infrared light is stimulated, the concentration of γ-aminobutyric acid in the rat brain is lower than that of the control group without stimulation. In addition, by performing Western blot test on neuronal core antigen (NeuN) in mouse brain neurons, it was found that NeuN had no significant difference between the test group and the control group. As shown in Figure 9, the protein band results of NeuN, T1-4 is the test group stimulated by infrared light, and C1-4 is the control group, only the optical fiber is inserted, but no stimulation.

As shown in FIG. 10 , it is a histogram of NeuN, the abscissa is the classification of the control group and the stimulation group, and the ordinate is the concentration value. The abscissa T is the concentration data of the test group stimulated by infrared light, and the abscissa C is the concentration data of the control group, only the optical fiber is inserted, but no stimulation; the ordinate represents the concentration. The analysis results suggest that the infrared light stimulation has no damage to the brain nerve tissue at the molecular level.

It can be seen from the test results that the device for stimulating the caudate nucleus in the brain to improve the excitability of the nervous system, based on the stimulating effect of infrared light on biological nerves, can reduce the concentration of γ-aminobutyric acid in the brain to improve the excitability of the nervous system , to relieve drowsiness and drowsiness caused by brain fatigue, and infrared light stimulation does not damage the brain nerves of rats, so it is safe and reliable.

By setting the front end of the puncture positioning part at the center of the caudate nucleus of the rat brain; causing the infrared point frequency light source module to generate infrared light, which passes through the optical fiber 203 and the outer sheath 302, and accurately irradiates the caudate nucleus, which can Efficiently, quickly and with low side effects, it solves the problems of fatigue, drowsiness and other problems caused by central nervous system depression caused by excessive secretion of γ-aminobutyric acid.

The invention can reduce the secretion of gamma-aminobutyric acid in the brain by introducing infrared light into the brain and stimulating the caudate nucleus of the brain, thereby improving the inhibitory state of the central nervous system of the human brain, relieving brain fatigue, and improving the excitability of the nervous system. The innovative point of this device is that it will not damage the central nervous system, and it will only cause a small wound to the individual when used. It is safe and reliable, and it is easy to heal after operation.

The device for stimulating the caudate nucleus in the brain to improve the excitability of the nervous system described in the embodiment of the present invention generates high-power infrared light with a wavelength of 8.5 μm and an optical power of 35 mW through an infrared point-frequency light source module; Based on the multi-stage focusing components to shrink the infrared light and process the focused beam to obtain the focused infrared light, the focused infrared light is collimated by the collimation component and then coupled into the optical fiber for transmission; the puncture guide component is used to guide the optical fiber The front end of the front end is guided to a specific irradiation stimulation site in the brain, and the infrared light coupled into the optical fiber is irradiated on the caudate nucleus of the brain, which can stimulate the caudate nucleus of the brain through infrared light to reduce the nerve damage caused by excessive secretion of γ-aminobutyric acid in the brain. System inhibition and fatigue, indirect control of the nervous system excitement of the organism, simple operation, safe and reliable, rapid regulation and short time.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: it can still be Modifications are made to the technical solutions described in the foregoing embodiments, or equivalent replacements are made to some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims (9)

1. An apparatus for stimulating the caudate nucleus in the brain to increase nervous system excitability, comprising:

the infrared spot frequency light source module is used for generating high-power infrared light with the wavelength of 8.5 microns and the light power of less than or equal to 200 mW;

the optical fiber coupling module is used for reducing and focusing the infrared light based on the multi-stage focusing component to obtain focused infrared light, and then the focused infrared light is collimated by the collimating component and then coupled into an optical fiber for conduction;

a puncture guide member for guiding the tip of the optical fiber to a specific irradiation stimulation site in the brain and irradiating the infrared light coupled into the optical fiber onto the caudate nucleus in the brain; wherein the irradiation stimulation site is caudate nucleus; the puncture guide member includes: an inner guide wire, an outer sheath and a fixed switch; the inner guide wire is used for puncturing an irradiation stimulation site entering the brain; the tail end of the inner guide wire is provided with a buckle corresponding to the clamping groove of the outer sheath, so that the inner guide wire can be buckled with the outer sheath and can be detachably withdrawn from the outer sheath in the puncture process; the outer sheath is used for puncturing under the guidance of the inner guide wire to place the irradiation stimulation site, and guiding the optical fiber to be placed into the irradiation stimulation site along the outer sheath after the inner guide wire is withdrawn from the outer sheath; the fixing switch is used for fixing the optical fiber in the sheath through mechanical pressure after being closed so as to enable the optical fiber to be fixedly arranged into a corresponding depth.

2. The apparatus of claim 1, wherein the switch forms a tubular channel for passing and fixing the optical fiber in the middle after being closed, and the inner surface of the tubular channel is made of soft material to protect the optical fiber from being damaged by mechanical pressure.

3. The apparatus of claim 1, wherein the switch is provided with a snap corresponding to a snap on the sheath to allow the switch to be engaged with and disengaged from the sheath during the process of fixing the optical fiber.

4. The device for stimulating the caudate nucleus within the brain to increase nervous system excitability of claim 1, wherein the outer sheath has an inner diameter of 1.1mm and an outer diameter of 1.3mm; the sheath is made of 70-80HD materials, is transparent in color and is provided with scales on the surface.

5. The device for stimulating the caudate nucleus within the brain to increase nervous system excitability of claim 1, wherein the inner guide wire is 0.8-0.9mm in diameter; the hardness of the material of the inner guide wire is 86-90HD.

6. The apparatus of claim 1, wherein the fiber coupling module comprises: the multi-stage focusing element, the collimating element and the optical fiber;

the multistage focusing component is used for reducing the light spot of the infrared light emitted by the infrared spot frequency light source module to enable the radius of the light spot of the infrared light to be reduced to be less than or equal to 600 mu m, and then the focused infrared light is obtained; the collimation component is used for collimating the focused infrared light so that the light beam irradiates along a straight line; the optical fiber is used for conducting the collimated infrared light.

7. The apparatus according to claim 6, wherein the optical fiber is a multimode optical fiber having an inner diameter of 580-610 μm, an outer diameter of 900-1000 μm, and a numerical aperture of 0.3, and is configured to efficiently conduct a light beam having a wavelength of 8.5 μm such that the power of the exit end of the optical fiber is 35mW.

8. The apparatus of claim 1, wherein the infrared light has a repetition frequency of 200kHz or less and a pulse width of 2 μ s or less.

9. The device of claim 1, wherein the device is configured to irradiate the infrared light on the caudate nucleus within the brain for a period of 5min.

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