CN115054554A

CN115054554A - Hispid fig washing-free hand sanitizer and preparation method thereof

Info

Publication number: CN115054554A
Application number: CN202210850495.1A
Authority: CN
Inventors: 杨琳; 张君诚; 洪玉莹; 张杭颖; 章立; 吴进华
Original assignee: Sanming University
Current assignee: Nanjing Si Nine Technology Services Co ltd
Priority date: 2022-07-20
Filing date: 2022-07-20
Publication date: 2022-09-16
Anticipated expiration: 2042-07-20
Also published as: CN115054554B

Abstract

The invention provides a hispid fig washing-free hand sanitizer and a preparation method thereof, wherein the method comprises the following steps: firstly, cleaning, drying and grinding the root of hispid fig to obtain hispid fig powder, then dissolving the hispid fig powder in ethanol, ultrasonically extracting, centrifuging and then removing precipitate to obtain an ethanol extract of the hispid fig; mixing the ethanol extract of hispid fig with glycerol to obtain the hispid fig no-clean hand sanitizer. The invention adopts 80% ethanol-ultrasonic method to extract active ingredients of the root of Ficus Simplicissima lour, and mixes the active ingredients with glycerol to prepare the non-washing hand sanitizer of Ficus Simplicissima lour. The five-finger wild peach is a faintly acid natural Chinese herbal medicine substance, and is added into the no-wash hand sanitizer to serve as an active ingredient, so that the concentration of ethanol in the no-wash hand sanitizer can be reduced, the damage of the ethanol to the skin of hands is reduced, and meanwhile, the antibacterial effect can be improved. In addition, the hispid fig can also endow the no-clean hand sanitizer with certain fragrance so as to improve the use feeling of users.

Description

Hispid fig washing-free hand sanitizer and preparation method thereof

Technical Field

The invention relates to the technical field of no-clean hand sanitizer, and particularly relates to a hispid fig no-clean hand sanitizer and a preparation method thereof.

Background

The no-clean hand sanitizer is famous without water in the cleaning process, so that the steps of foaming and cleaning can be saved, and the no-clean hand sanitizer is convenient, practical and safe and has the function which is not inferior to the conventional hand sanitizer. For example, in general, the contact surface between hands and the non-washing hand lotion is the pump head of a bottle, unlike soap, perfumed soap and other solid which are easy to be contaminated and sterilized on the hands, the non-washing hand lotion is easy to clean and can avoid causing secondary infection. The no-wash hand sanitizer has obvious sterilization effect, has the sterilization rate of over 90 percent on candida albicans, staphylococcus aureus, escherichia coli, hepatitis A and other pathogens, has no irritation and anaphylactic reaction to a human body, and can effectively protect family and self from bacteria and diseases. More importantly, the washing-free hand sanitizer is not used with water, can be sprayed on hands for several times and repeatedly rubs hands, is very convenient to use, and is often used in water-free hand washing-free disinfection environments such as hospitals, restaurants, families, offices, enterprises and public institutions, railway stations, tourism, troops, entertainment venues, schools, kindergartens, supermarkets, hotels, banks, airports, docks, cinemas and the like.

At present, the main component of most of no-clean hand washing solutions is alcohol. The basic formula of the alcohol-containing no-clean hand sanitizer is generally alcohol plus glycerin, and is added with some essence for regulating fragrance. Wherein, alcohol is used as a disinfection component, and automatically volatilizes after acting on hands to play a disinfection role, no water is needed for washing, the requirement of quick drying can be met, and glycerin keeps certain moisture on the skin to play a role in moisturizing the skin. With the increasing importance of consumers on hand hygiene and skin protection, the market of the no-clean hand sanitizer is continuously expanding, and the no-clean hand sanitizer has a certain application prospect. In order to achieve the effects of sterilization and bacterial growth inhibition, the general no-clean hand sanitizer contains about 78% of ethanol, has irritation and damage to the skin of hands after long-term use, and has strong pungent smell. But the content can be reduced to achieve the expected bacteriostatic effect. Therefore, on the premise of not reducing the bacteriostatic action of the no-clean hand sanitizer, reducing the alcohol content of the no-clean hand sanitizer becomes a hot problem for researching the no-clean hand sanitizer. Furthermore, one reason why the skin is resistant to microorganisms is that some substances present on the surface of the skin are acidic and the skin ph of the hands of adults and teenagers is less than 5. When the no-clean hand sanitizer is alkaline or neutral, the skin of hands feels tight and dry after hand washing. The reason is that the hand sanitizer does not destroy substances with acidity on the skin surface, and the pH value on the skin surface is increased to directly or indirectly influence the metabolism of keratinocytes, so that the symptoms of proliferation and differentiation of the keratinocytes, change of the skin barrier function, reduction of the stratum corneum compactness and the like are caused.

Disclosure of Invention

The invention aims to provide the hispid fig washing-free hand sanitizer which has lower ethanol content and better antibacterial effect.

The invention also aims to provide a preparation method of the hispid fig no-clean hand sanitizer, which can reduce the ethanol content in the no-clean hand sanitizer and increase the antibacterial effect by adding the active component of the hispid fig in the no-clean hand sanitizer, has simple operation and controllable parameters, and is suitable for industrial large-scale production.

The technical problem to be solved by the invention is realized by adopting the following technical scheme.

The invention provides a preparation method of a hispid fig washing-free hand sanitizer, which comprises the following steps:

s1, preparing an ethanol extract of hispid fig: cleaning root of hispid fig, drying, grinding to obtain hispid fig powder, dissolving the hispid fig powder in ethanol, ultrasonic extracting, centrifuging, and removing precipitate to obtain hispid fig ethanol extractive solution;

s2, mixing the hispid fig alcohol extract with glycerol to obtain the hispid fig washing-free hand sanitizer.

The invention provides a hispid fig washing-free hand sanitizer, which is prepared according to the preparation method.

The non-washing hand sanitizer containing hispid fig and the preparation method thereof have the advantages that:

the invention adopts 80% ethanol-ultrasonic method to extract active ingredients of the root of Ficus Simplicissima lour, and mixes the active ingredients with glycerol to prepare the non-washing hand sanitizer of Ficus Simplicissima lour. The five-finger wild peach is a faintly acid natural Chinese herbal medicine substance, and is added into the no-wash hand sanitizer to serve as an active ingredient, so that the concentration of ethanol in the no-wash hand sanitizer can be reduced, the damage of the ethanol to the skin of hands is reduced, and meanwhile, the antibacterial effect can be improved. In addition, the hispid fig can also endow the no-clean hand sanitizer with certain fragrance so as to improve the use feeling of users.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and those skilled in the art can also obtain other related drawings based on the drawings without inventive efforts.

FIG. 1 is a graph showing the variation of ph of the hispid fig. 1-3 hand sanitizer of the present invention and the ethanol hand sanitizer of comparative example 1;

FIG. 2 is a diagram of Escherichia coli culture media in which small round paper sheets containing hispid fig washing-free hand sanitizer and hispid fig alcohol extract, glycerol and ethanol washing-free hand sanitizer are respectively placed;

FIG. 3 is a graph showing the growth of E.coli in five media containing hispid fig washing-free hand sanitizer, hispid fig alcohol extract, glycerol, ethanol washing-free hand sanitizer and pure LB media, respectively;

FIG. 4 is a graph showing the growth of E.coli in three media containing hispid fig washing-free hand sanitizer, hispid fig alcohol extract and ethanol washing-free hand sanitizer, respectively;

FIG. 5 is a flow chart of the preparation of the hispid fig washing-free hand sanitizer of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are conventional products which are not indicated by manufacturers and are commercially available.

The hispid fig washing-free hand sanitizer and the preparation method thereof of the embodiment of the invention are specifically described below.

Referring to fig. 5, the preparation method of the hispid fig washing-free hand sanitizer provided by the embodiment of the present invention includes the following steps:

s1, preparing an ethanol extract of hispid fig: cleaning root of hispid fig, drying, grinding to obtain hispid fig powder, dissolving the hispid fig powder in ethanol, ultrasonic extracting, centrifuging, and removing precipitate to obtain hispid fig ethanol extractive solution. The fructus fici simplicissimae ethanol extract is a weakly acidic natural Chinese herbal medicine substance, and is added into the no-wash hand sanitizer as an active substance, so that the no-wash hand sanitizer has a bacteriostatic action and cannot damage substances with acidity on the surface of skin, and the skin feel is not greasy and tight.

Further, in a preferred embodiment of the present invention, the drying and grinding steps include: and drying the cleaned roots of the hispid fig until the weight is constant, and then putting the roots of the hispid fig into a crusher for grinding to obtain the hispid fig powder.

Further, in a preferred embodiment of the present invention, the mass percentage of the ethanol is 75 to 85%, and the mass volume ratio of the hispid fig powder to the ethanol is 1: 15-25 (g/mL).

Further, in the preferred embodiment of the present invention, the temperature of the ultrasonic extraction is 35 to 39 ℃, and the extraction time is 50 to 70 min.

Further, in a preferred embodiment of the present invention, the centrifugation comprises the following specific steps: and putting the mixed liquid of the hispid fig powder and the ethanol after ultrasonic extraction into a freezing high-speed centrifuge, and centrifuging for 10-20 min at 7500-8500 r at 3-5 ℃.

Further, in a preferred embodiment of the present invention, the volume ratio of the hispid fig alcoholic extract to the glycerin is 3 to 7: 1.

further, in a preferred embodiment of the invention, in the hispid fig no-clean hand sanitizer, the mass percentage of the ethanol is 60-70%.

The invention adopts 80% ethanol-ultrasonic method to extract active ingredients of the root of Ficus Simplicissima lour, and mixes the active ingredients with glycerol to prepare the non-washing hand sanitizer of Ficus Simplicissima lour. The five-finger wild peach is a faintly acid natural Chinese herbal medicine substance, and is added into the no-wash hand sanitizer to serve as an active ingredient, so that the concentration of ethanol in the no-wash hand sanitizer can be reduced, the damage of the ethanol to the skin of hands is reduced, and meanwhile, the antibacterial effect is improved. In addition, the hispid fig can also endow the no-clean hand sanitizer with certain fragrance so as to improve the use feeling of users.

The invention also provides the hispid fig washing-free hand sanitizer which is prepared according to the preparation method.

The features and properties of the present invention are described in further detail below with reference to examples.

Example 1

The hispid fig washing-free hand sanitizer provided by the embodiment is prepared according to the following method:

(1) preparing an ethanol extract of hispid fig: cleaning the root of hispid fig, drying to constant weight, and grinding into powder in a crusher. Then taking 5g of ground root of hispid fig into powder, dissolving in 100mL of 80% ethanol, ultrasonically extracting for 60min at 37 ℃, placing in a freezing high-speed centrifuge for 15min at 4 ℃ and 8000r, and removing the precipitate to obtain the hispid fig ethanol extract.

(2) Preparing the hispid fig washing-free hand sanitizer: according to the volume ratio of the ethanol extract of the hispid fig to the glycerin of 3:1, preparing to obtain the hispid fig washing-free hand sanitizer. Wherein, in the hispid fig washing-free hand sanitizer, the content of ethanol is 60 percent.

Example 2

(2) Preparing the hispid fig washing-free hand sanitizer: according to the volume ratio of the ethanol extract of the hispid fig to the glycerin of 5: 1, preparing to obtain the hispid fig washing-free hand sanitizer. Wherein, in the hispid fig washing-free hand sanitizer, the content of ethanol is 66 percent.

Example 3

(1) preparing an ethanol extract of hispid fig: cleaning the root of hispid fig, drying to constant weight, and grinding the root of hispid fig into powder in a crusher for later use. Then taking 5g of ground root of hispid fig into powder, dissolving in 100mL of 80% ethanol, ultrasonically extracting for 60min at 37 ℃, placing in a freezing high-speed centrifuge for 15min at 4 ℃ and 8000r, and removing the precipitate to obtain the hispid fig ethanol extract.

(2) Preparing the hispid fig washing-free hand sanitizer: according to the volume ratio of the ethanol extract of the hispid fig to the glycerin of 7: 1, preparing to obtain the hispid fig washing-free hand sanitizer. Wherein, in the hispid fig washing-free hand sanitizer, the content of ethanol is 70 percent.

Comparative example 1

The comparative example provides an ethanol no-clean hand sanitizer, wherein the ethanol in the ethanol no-clean hand sanitizer accounts for 80% by mass, and the ratio of ethanol to glycerin is 7: 1.

test example 1

The test example is a comparison test of the ph value of the hispid fig washing-free hand sanitizer in examples 1 to 3 and the ph value of the ethanol washing-free hand sanitizer in comparative example 1 with time, and specifically comprises the following steps:

firstly, the hispid fig washing-free hand sanitizer in the embodiment 1-3 and the ethanol washing-free hand sanitizer in the comparative example 1 are respectively poured into beakers and numbered from the first to the fourth. Wherein, the ratio of the hispid fig alcohol extract to the glycerin in the embodiment 1 is 3: the ratio of the washing-free hand sanitizer 1, the hispid fig ethanol extract of example 2 and the glycerin is 5: the ratio of the non-washing hand sanitizer of 1 to the hispid fig alcohol extract of embodiment 3 to glycerin is 7: 1, the ratio of 80% ethanol to glycerin of comparative example 1 is 7: 1 the no-clean hand sanitizer. Then testing the pH of the five-finger wild peach washing-free hand sanitizer with the three formulas and the control group of the ethanol washing-free hand sanitizer with the three formulas at 0, 1, 3, 6, 12, 24, 48, 96 and 144h respectively, measuring three times at each time point, taking the time when the pH is measured as an abscissa and the average pH value of the measured solution as an ordinate, and drawing a line graph of the change of the pH values of the four washing-free hand sanitizers in 6d (144h) along with the time.

FIG. 1 shows the graph of the change of ph of the hispid fig washing-free hand sanitizer of examples 1-3 and the ethanol washing-free hand sanitizer of comparative example 1. As can be seen from fig. 1, PH of the No. 1 No. i to No. iii alcohol No. i hand sanitizers containing hispid fig active ingredients are acidic, and PH of the No. i alcohol No. i hand sanitizers containing no hispid fig active ingredients are alkaline. The ph change trends of the four groups of hand lotions with different formulas are all descending. In the first 48 hours, the ph of the No. one and No. four no-clean hand cleaners is reduced by 0.65 (9.84%) and 0.67 (8.51%) respectively faster than that of the No. three no-clean hand cleaners; however, in the time period of 48-144 h, the ph of the no-clean hand sanitizer is remarkably more stable than that of the no-clean hand sanitizer. Within 48-96 hours, the ph of the No. one no-clean hand sanitizer is only reduced by 0.08 (1.37%), and almost no difference exists before 48 hours; but the reduction of No. 2, No. 3 and No. 4 is more, and the reduction is respectively 0.20 (3.33%), 0.17 (2.78%) and 0.15 (2.08%). In 96-144 hours, the numbers I, II, III and IV respectively decrease by 0.08 (1.38%), 0.12 (2.07%), 0.21 (3.53%) and 0.27 (3.83%). Therefore, the stability of the No. I and No. II no-clean hand sanitizer is better than that of the No. III and No. IV in the period, wherein the stability of the No. I no-clean hand sanitizer is slightly better than that of the No. II hand sanitizer.

Test example 2

In this test example, changes in the appearance of the hispid fig leave-on hand sanitizer and the ethanol leave-on hand sanitizer 6d (144h) in test example 1 were observed, and the results are shown in table 1.

TABLE 1 appearance change table of no-clean hand sanitizer

As can be seen from Table 1, the hand sanitizer and glycerin are well soluble in each other, no delamination and no precipitate are generated, while the hand sanitizer of No. III has the precipitate phenomenon, and the solution is not as stable as the hand sanitizer of No. I.

Test example 3

The results of this test example, in which the skin feel of the hispid fig leave-on hand sanitizer and the ethanol leave-on hand sanitizer in test example 1 were recorded, are shown in table 2.

TABLE 2 sensory evaluation of leave-on hand sanitizer

As can be seen from the table 2, the No. I no-clean hand sanitizer has no greasy feeling or tight feeling after being used, has good skin feel, is slightly greasy both No. II and No. III, and is also superior to the No. II and No. III.

In summary, from the experimental examples 1 to 3, it can be known that the formula of the no-clean hand sanitizer of hispid fig is an alcohol extract: the pH of the no-clean hand sanitizer containing 3:1 glycerin is acidic, and the no-clean hand sanitizer is stable, free of layering and precipitation, free of greasy skin feel and tight feel, and good in comprehensive evaluation.

Test example 4

In the test example, the in-vitro antibacterial action of the hispid fig washing-free hand sanitizer and the hispid fig alcohol extract, glycerol and ethanol washing-free hand sanitizer is explored by adopting a circular filter paper disc method, and the specific steps are as follows:

sterile small circular filter paper pieces having a diameter of 6mm prepared in advance were put into the four beakers of table 3, respectively, and soaked for 2 hours. Then, the single clone was picked up and placed in 1ml of LB medium and cultured with shaking at 37 ℃ and 180r for 40 min. The activated bacteria are inoculated on a solid culture medium by a coating method. And then dividing a culture medium into four areas, sequentially pasting small round filter paper sheets soaked in liquid in No. 1-4 beakers to different areas, sealing and marking. And (3) culturing the coated plates at 37 ℃ for 8h, observing, measuring and counting the size of the inhibition zone of the small disc on each plate, and comparing the inhibition effect of various hand washing solutions and various components thereof on escherichia coli. Wherein, the size of the inhibition zone is shown in table 4.

TABLE 3 combination of different ingredients of no-clean hand sanitizer in four beakers

FIG. 2 shows a diagram of Escherichia coli culture medium containing small round paper sheets containing hispid fig washing-free hand sanitizer, hispid fig alcohol extract, glycerol and ethanol washing-free hand sanitizer. As can be seen from FIG. 2, the Escherichia coli culture medium placed with small circular paper sheets is cultured for 8h, and inhibition zones appear on the plates.

TABLE 4 bacteriostatic effect of different components on E.coli

It can be known from tables 3 and 4 that the components soaked by the number 1 and number 2 round filter paper sheets with larger inhibition zones are the hispid fig washing-free hand sanitizer and the hispid fig alcohol extract respectively, the number 3 round filter paper sheet soaked with glycerin has no inhibition zone, and the number 4 round filter paper sheet soaked with the alcohol washing-free hand sanitizer has inhibition zones, but the inhibition zones are smaller than the number 1 and number 2. By comparing the sizes of the inhibition zones, the best inhibition effect and obvious inhibition zone of the no-wash hand sanitizer containing the active components of the hispid fig and the ethanol extract can be preliminarily obtained; the bacteriostatic effect of the ethanol non-washing hand sanitizer is inferior; glycerol has no bacteriostatic effect.

Test example 5

In the test example, the ultraviolet-visible spectrophotometer method is adopted to explore the in vitro antibacterial action of the hispid fig washing-free hand sanitizer, the hispid fig alcohol extract, the glycerol and ethanol washing-free hand sanitizer, and the specific steps are as follows:

and taking four 250mL conical flasks, respectively putting 170mL of prepared LB liquid culture medium into each of the four conical flasks, respectively adding 30mL of hispid fig washing-free hand sanitizer, hispid fig alcohol extract, glycerol and ethanol washing-free hand sanitizer into the corresponding 250mL conical flasks, and fully mixing and shaking. Another 250mL conical flask was placed in 200mL LB liquid medium as a control. Escherichia coli was inoculated into 20mL of a liquid medium and cultured at 37 ℃ and 180rpm for 90 min. Respectively adding 2 mL of bacterial liquid into the prepared culture medium added with different antibacterial components, culturing at 37 ℃ and 180rpm, and respectively detecting the OD value of escherichia coli in the culture bottle every 40 min. Each set of experiments had three biological replicates and three technical replicates.

And respectively testing the OD600 values of escherichia coli cultured in LB culture media with different bacteriostatic components for 0min, 100min, 140 min, 160min, 180 min, 200 min and 220min and a negative control group, measuring for three times at each time point, taking the culture time of the escherichia coli as a horizontal coordinate, taking the average OD600 value of the bacteria liquid measured at each time point as a vertical coordinate, and drawing growth curves of the escherichia coli in different culture media.

FIG. 3 shows the growth curve of Escherichia coli in five culture media respectively containing hispid fig washing-free hand sanitizer, hispid fig alcohol extract, glycerol, ethanol washing-free hand sanitizer and pure LB culture medium. In fig. 3, nos. 1 to 4 are culture solutions to which hispid fig washing-free hand sanitizer, hispid fig alcohol extract, glycerin and ethanol washing-free hand sanitizer are added, and No. 5 is a negative control group, i.e., a culture medium without any antibacterial component. As can be seen from FIG. 3, the phases of growth of E.coli in the negative control group are clearly distinguished, and the species used in this test are normal. 0-100 min is a lag phase, and the propagation speed is slow because the escherichia coli adapts to a new culture environment; 100-170 min is in logarithmic growth phase, the base number of the escherichia coli is proper, the nutrients are sufficient, and the escherichia coli rapidly grows in logarithmic growth phase; 180-220 min is a stable period, at this time, not only a few nutrients are left in the culture solution and are continuously consumed, but also the toxic substances accumulated in the metabolism of the escherichia coli continuously rise, and the propagation speed of the escherichia coli gradually decreases under the influence of factors such as ph decrease, the number of dead escherichia coli begins to increase, the proliferation number and the death number gradually tend to be balanced, and the total number of cells also tends to be stable. Due to the fact that the culture time is too short, the growth curve in the graph 3 does not evolve to the decay stage of escherichia coli, but does not influence the research on the antibacterial effect of the components of the hispid fig washing-free hand sanitizer in the test.

The glycerol group 3 in FIG. 3 also shows the slow, fast and stable growth period of E.coli. However, the OD600 value in the bacterial suspension No. 3 was low compared to that in the bacterial suspension No. 5, which means that the growth and propagation ability of Escherichia coli in the bacterial suspension No. 3 was inferior to that in the control group No. 5. While OD600 values of No. 1, No. 2 and No. 4 were always around 0, almost no Escherichia coli growth occurred, indicating that 30mL of the three substances added to the medium all had a strong bacteriostatic effect. In conclusion, the bacteriostatic effects of No. 1, No. 2 and No. 4 are far greater than those of the glycerin group and the negative group, and strong contrast is formed between the glycerin group and the negative group, but the strength of the bacteriostatic effects of No. 1, No. 2 and No. 3 cannot be obtained by the experiment.

Test example 6

The experimental example researches the bacteriostatic effect of the hispid fig washing-free hand sanitizer, the hispid fig alcohol extract and the ethanol washing-free hand sanitizer added into a culture medium to explore the strength of the bacteriostatic effect among the hispid fig washing-free hand sanitizer, and the specific steps are as follows:

respectively adding 0.5mL of hispid fig washing-free hand sanitizer, hispid fig alcohol extract and ethanol washing-free hand sanitizer into 30mL of prepared LB culture medium, marking as No. 1, No. 2 and No. 3, transferring 0.3 mL of bacterial liquid to culture at 37 ℃, measuring the OD600 values of escherichia coli bacterial liquid and a negative control group which are cultured for 0, 60, 100, 120, 140, 150 and 160min, measuring three times at each time point, taking the culture time of escherichia coli as a horizontal coordinate, taking the average OD600 value of the bacterial liquid measured at each time point as a vertical coordinate, and drawing the growth curves of the escherichia coli in the three culture media.

FIG. 4 is a graph showing the growth of E.coli in three media containing hispid fig washing-free hand sanitizer, hispid fig alcohol extract and ethanol washing-free hand sanitizer. As can be seen from FIG. 4, the growth curve of Escherichia coli with the added antibacterial components in the bacterial liquid no longer conforms to the growth curve of normal Escherichia coli in FIG. 3, and the bacteriostatic effect of three antibacterial components can be distinguished only in the time zone of 60 min-150 min. By analyzing the OD600 values of the three curves at different times, the antibacterial effect of the hispid fig alcohol extract is judged to be the best, the antibacterial effect of the hispid fig non-washing hand sanitizer is the second best, and the antibacterial effect of the ethanol non-washing hand sanitizer is the worst.

The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without inventive step based on the embodiments of the present invention, are within the scope of protection of the present invention.

Claims

1. A preparation method of a hispid fig washing-free hand sanitizer is characterized by comprising the following steps:

s1, preparing an ethanol extract of hispid fig: cleaning, drying and grinding roots of hispid fig to obtain hispid fig powder, dissolving the hispid fig powder in ethanol, ultrasonically extracting, centrifuging, and removing precipitate to obtain hispid fig ethanol extract;

2. The method of claim 1, wherein in step S1, the steps of drying and grinding include: drying the cleaned root of the hispid fig until the weight is constant, and then putting the root into a crusher for grinding to obtain the hispid fig powder.

3. The preparation method according to claim 1, wherein in step S1, the mass percentage of the ethanol is 75-85%, and the mass volume ratio of the hispid fig powder to the ethanol is 1: 15-25 (g/mL).

4. The method according to claim 1, wherein in step S1, the temperature of ultrasonic extraction is 35-39 ℃ and the extraction time is 50-70 min.

5. The method according to claim 1, wherein in step S1, the centrifugation step comprises: and putting the mixed liquid of the hispid fig powder and the ethanol after ultrasonic extraction into a freezing high-speed centrifuge, and centrifuging for 10-20 min at 7500-8500 r at 3-5 ℃.

6. The method according to claim 1, wherein in step S2, the volume ratio of the hispid fig alcoholic extract to the glycerin is 3-7: 1.

7. the preparation method of claim 1, wherein the mass percent of the ethanol in the hispid fig no-clean hand sanitizer is 60-70%.

8. A hispid fig no-clean hand sanitizer is characterized by being prepared by the preparation method of any one of claims 1 to 7.