CN115032133B - Endothelial cell monolayer permeability detection experimental device - Google Patents
Endothelial cell monolayer permeability detection experimental device Download PDFInfo
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- CN115032133B CN115032133B CN202210628587.5A CN202210628587A CN115032133B CN 115032133 B CN115032133 B CN 115032133B CN 202210628587 A CN202210628587 A CN 202210628587A CN 115032133 B CN115032133 B CN 115032133B
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- 230000035699 permeability Effects 0.000 title claims abstract description 26
- 238000001514 detection method Methods 0.000 title claims abstract description 23
- 210000002889 endothelial cell Anatomy 0.000 title claims abstract description 21
- 239000013553 cell monolayer Substances 0.000 title claims abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 65
- 238000002955 isolation Methods 0.000 claims abstract description 47
- 239000003814 drug Substances 0.000 claims abstract description 29
- 238000012360 testing method Methods 0.000 claims abstract description 24
- 230000007246 mechanism Effects 0.000 claims description 64
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 35
- 229920000742 Cotton Polymers 0.000 claims description 22
- 230000001954 sterilising effect Effects 0.000 claims description 13
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 238000004140 cleaning Methods 0.000 claims description 8
- 230000000903 blocking effect Effects 0.000 claims description 7
- 230000001681 protective effect Effects 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 2
- 244000309464 bull Species 0.000 claims 4
- 239000011521 glass Substances 0.000 abstract description 15
- 241000894006 Bacteria Species 0.000 abstract description 6
- 239000002356 single layer Substances 0.000 description 5
- 230000009471 action Effects 0.000 description 4
- 230000003511 endothelial effect Effects 0.000 description 4
- 238000000691 measurement method Methods 0.000 description 4
- 239000006096 absorbing agent Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000002792 vascular Effects 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229920006289 polycarbonate film Polymers 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/08—Investigating permeability, pore-volume, or surface area of porous materials
- G01N15/0806—Details, e.g. sample holders, mounting samples for testing
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/20—Gaseous substances, e.g. vapours
- A61L2/208—Hydrogen peroxide
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Physics & Mathematics (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Dispersion Chemistry (AREA)
- Epidemiology (AREA)
- Analytical Chemistry (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a detection experimental device, in particular to an endothelial cell monolayer permeability detection experimental device. The invention aims to provide an endothelial cell monolayer permeability test device which can be automatically operated and kill residual bacteria. The invention provides an endothelial cell monolayer permeability detection experimental device which comprises a base, an isolation cover, a movable door and a conductivity tester, wherein the top of the base is connected with the isolation cover, the front part of the isolation cover is rotatably connected with the movable door, the conductivity tester is used for experimental detection, the device also comprises a display and the like, and the upper part of the front side of the isolation cover is connected with the display. According to the invention, the first motor and the second motor are started, the electric liquid suction device and the electric sucking disc can be controlled to rotate and move, then the electric liquid suction device and the electric sucking disc are started, so that the liquid medicine can be automatically fed, and the transwell chamber is automatically placed in the glass cup, thereby facilitating detection of people in a closed space.
Description
Technical Field
The invention relates to a detection experimental device, in particular to an endothelial cell monolayer permeability detection experimental device.
Background
Currently, it is often difficult to measure the permeability of the vascular wall of a human body, and it is difficult to observe the influence of a single factor on the permeability of the vascular wall due to the influence of various complex factors in a receptor. Therefore, in vitro measurement of the endothelial permeability of the monolayer becomes a main means for researching the endothelial monolayer permeability function, compared with an albumin filtration measurement method, the conductivity measurement method of the endothelial permeability of the monolayer is simpler to operate, and the technical sensitivity and the sensibility are obviously improved.
However, in the experiment, the conductivity measurement method also needs to be operated manually step by step, and since a good isolation effect is difficult to achieve in the manual operation, endothelial cells are easy to be polluted by bacteria, and before the experiment, people need to open a sterile room and put various experimental equipment into the sterile room, so that external air is easy to enter the sterile room, and the detection result is influenced.
Thus, in response to the above-described shortcomings, an endothelial cell monolayer permeability test device is provided that is capable of automated operation and killing residual bacteria.
Disclosure of Invention
In order to overcome the defects that when people carry out a conductivity measurement method of single-layer endothelial permeability, manual operation is needed, and bacteria easily enter a sterile room and stay in the sterile room when various experimental equipment is put in, so that the detection result is influenced, the invention aims to provide an endothelial cell single-layer permeability detection experimental device which can be automatically operated and kill residual bacteria.
The invention is realized by the following technical approaches:
The utility model provides an endothelial cell monolayer permeability detection experimental apparatus, the on-line screen storage device comprises a base, the cage, dodge gate and conductivity tester, the base top is connected with the cage, the anterior rotation of cage is connected with the dodge gate, the conductivity tester is used for experimental detection, still including the display, first rack, the second rack, control mechanism, a test mechanism, sterilization mechanism and isolation mechanism, cage front side upper portion is connected with the display, base top front side right part is connected with first rack, base top rear side right part is connected with the second rack, first rack and second rack all are located the inside of cage, the base upside is equipped with the control mechanism that is used for automatic material preparation, base top rear side middle part is equipped with the test mechanism that is used for making the conductivity tester remove, the conductivity tester installs on test mechanism, conductivity tester passes through electric connection with the display, be equipped with the isolation mechanism that is used for blocking experimental instrument between first rack and the second rack, through with experimental apparatus on first rack and the second rack, make isolation cage and activity gate be connected with the second rack, make the cage and the second rack pass through the control mechanism that is used for making the sealed space after the test instrument is used for detecting the liquid medicine, the sealed instrument, the data is accomplished through the sealed instrument that is passed through to the sealed instrument, and is used for detecting the sealed instrument.
In one embodiment, the control mechanism comprises a first motor, a guide frame, a second motor, a first screw rod, a first movable plate, an electric liquid suction device and an electric sucking disc, wherein the right side of the middle part of the upper side of the base is connected with the first motor, the right side of the middle part of the upper side of the base is rotatably connected with the guide frame, the bottom of the guide frame is connected with the output shaft of the first motor, the top of the guide frame is connected with the second motor, the guide frame is rotatably connected with the first screw rod, the top of the first screw rod is connected with the output shaft of the second motor, the guide frame is connected with the first movable plate in a sliding mode, the first movable plate is in threaded connection with the first screw rod, the left side of the first movable plate is connected with the electric liquid suction device, the right side of the first movable plate is connected with the electric sucking disc, the electric liquid suction device and the electric sucking disc are driven to rotate and move by opening the first motor and the second motor, and the electric liquid suction device and the electric sucking disc are automatically prepared by opening.
In one embodiment, the testing mechanism comprises a supporting frame, a third motor, a second screw rod and a second movable plate, wherein the middle part of the rear side of the top of the base is connected with the supporting frame, the top of the supporting frame is connected with the third motor, the second screw rod is connected with the inside of the supporting frame in a rotating mode, the top of the second screw rod is connected with the output shaft of the third motor, the second screw rod is connected with the second movable plate in a threaded mode, the second movable plate is connected with the supporting frame in a sliding mode, the front part of the second movable plate is connected with the conductivity tester, the second screw rod is driven to rotate through the opening of the third motor, and the second movable plate and the conductivity tester are enabled to move.
In one embodiment, the sterilization mechanism comprises an annular rack, a sliding block, a fourth motor, a first gear and a hydrogen peroxide sterilizer, wherein the upper part of the inner side of the isolation cover is connected with the annular rack, the inner side of the annular rack is connected with the sliding block in a sliding manner, the upper part of the sliding block is connected with the fourth motor, the output shaft of the fourth motor is connected with the first gear, the first gear is meshed with the annular rack, the front side of the sliding block is connected with the hydrogen peroxide sterilizer, the hydrogen peroxide sterilizer is started through the fourth motor, the first gear is driven to rotate, the sliding block and the hydrogen peroxide sterilizer move, and the hydrogen peroxide sterilizer is started to sterilize the inside of the isolation cover.
In one embodiment, the isolation mechanism comprises a rotating rod, a baffle, an elastic piece, a second gear and a toothed plate, wherein the outer sides of the tops of the first rack and the second rack are respectively connected with the rotating rod in a rotating mode, the baffle is connected to the lower side of the rotating rod, the elastic piece is connected between the baffle on the front side and the first rack, the elastic piece is also connected between the baffle on the rear side and the second rack, the upper side of the rotating rod is connected with the second gear through a one-way clutch, the toothed plate is connected to the upper left side of the guide frame and the right side of the top of the guide frame, the toothed plate is contacted with the second gear when rotating, the baffle is pushed to rotate, and then the experimental instrument is placed, and the elastic piece is used for resetting the baffle to shield the experimental instrument.
In one embodiment, the cleaning mechanism for wiping the isolation cover is further arranged, the cleaning mechanism comprises a supporting rod and wiping cotton, the annular chute is located inside the isolation cover, the lower portion of the rear side of the sliding block is connected with the supporting rod, the rear side of the supporting rod is connected with the wiping cotton, the wiping cotton is in contact with the inner wall of the isolation cover, and the wiping cotton is enabled to wipe the inner wall of the isolation cover through movement of the supporting rod and the wiping cotton.
In one embodiment, the device further comprises a blocking mechanism for protecting, the blocking mechanism comprises a protecting shell and a rubber sleeve, the upper part of the inner side of the annular rack is connected with the protecting shell, the outer side of the protecting shell is connected with the isolation cover, the guide frame is connected with the rubber sleeve, the left side and the right side of the first movable plate penetrate through the rubber sleeve, and protection is achieved on the fourth motor and the first screw rod through the protecting shell and the rubber sleeve.
In one embodiment, the cage is transparent.
In one embodiment, the elastic member is a torsion spring.
In one embodiment, the outer side of the top of the base is provided with an annular chute, and the support rod slides in the annular chute.
The invention provides an endothelial cell monolayer permeability detection experimental device, which has the advantages that:
1. after the glass cup, the liquid medicine pipe filled with the liquid medicine and the transwell chamber are placed, the hydrogen peroxide sterilizer is started, so that the inside of the isolation cover can be sterilized, and the influence of residual bacteria in the isolation cover on a detection result is prevented.
2. According to the invention, the first motor and the second motor are started, the electric liquid suction device and the electric sucking disc can be controlled to rotate and move, then the electric liquid suction device and the electric sucking disc are started, so that the liquid medicine can be automatically fed, and the transwell chamber is automatically placed in the glass cup, thereby facilitating detection of people in a closed space.
3. Under the action of the wiping cotton, the wiping cotton moves in an annular shape, so that the wiping cotton can wipe the inner wall of the isolation cover, and people can watch the internal condition through the isolation cover conveniently.
Drawings
Fig. 1 is a schematic perspective view of the present invention.
Fig. 2 is a schematic view of a partial perspective structure of the present invention.
Fig. 3 is a schematic perspective view of a testing mechanism according to the present invention.
Fig. 4 is a schematic perspective view of a portion of a testing mechanism according to the present invention.
Fig. 5 is a schematic perspective view of a control mechanism according to the present invention.
Fig. 6 is a schematic view of a partial perspective structure of a control mechanism according to the present invention.
Fig. 7 is a schematic perspective view of an isolation mechanism according to the present invention.
Fig. 8 is a schematic view of a partial perspective structure of an isolation mechanism according to the present invention.
Fig. 9 is a schematic perspective view of a sterilization mechanism according to the present invention.
Fig. 10 is a schematic perspective view of a cleaning mechanism according to the present invention.
Fig. 11 is a schematic perspective view of a blocking mechanism according to the present invention.
Marked in the figure as: 1-base, 2-cage, 3-movable door, 4-display, 5-first rack, 6-second rack, 7-conductivity tester, 8-control mechanism, 81-first motor, 82-guide frame, 83-second motor, 84-first screw, 85-first movable plate, 86-electric aspirator, 87-electric suction cup, 9-test mechanism, 91-support frame, 92-third motor, 93-second screw, 94-second movable plate, 10-sterilization mechanism, 101-annular rack, 102-slider, 103-fourth motor, 104-first gear, 105-hydrogen peroxide sterilizer, 11-isolation mechanism, 1101-rotating rod, 1102-baffle, 1103-elastic member, 1104-second gear, 1105-toothed plate, 12-cleaning mechanism, 1201-annular chute, 1202-support rod, 1203-wiping cotton, 13-blocking mechanism, 1301-protective shell, 1302-rubber sleeve, 14-glass cup, 15-liquid medicine tube, 16-transwell chamber.
Detailed Description
The invention is further elucidated below in conjunction with the drawings of the specification, and embodiments of the invention are given in conjunction with the drawings of the specification.
Example 1
The utility model provides an endothelial cell monolayer permeability detects experimental apparatus, including base 1, the cage 2, dodge gate 3, the display 4, first rack 5, second rack 6, conductivity tester 7, control mechanism 8, test mechanism 9, sterilization mechanism 10 and isolation mechanism 11, see that the 1 top outside of base is connected with cage 2 in FIG. 1-FIG. 9, cage 2 is transparent material, cage 2 front side lower part swivelling joint has dodge gate 3, cage 2 front side upper portion is connected with display 4, open through display 4, can make things convenient for people to watch detected data, 1 top front side right part of base is connected with first rack 5, 1 top rear side right part of base is connected with second rack 6, first rack 5 and second rack 6 all are located the inside of cage 2, 1 top rear side middle part of base is equipped with test mechanism 9, be connected with tester 7 on the test mechanism 9, can begin to detect through conductivity tester 7, conductivity tester 7 and display 4 are equipped with between the electrical property rack 5 and the second rack 6 through being connected with on the cage 2, the second rack 6 is equipped with between the first rack 11.
The control mechanism 8 comprises a first motor 81, a guide frame 82, a second motor 83, a first screw rod 84, a first movable plate 85, an electric liquid suction device 86 and an electric sucking disc 87, the middle right side of the upper side of the base 1 is bolted with the first motor 81, the middle right side of the upper side of the base 1 is rotatably connected with the guide frame 82, the bottom of the guide frame 82 is connected with the output shaft of the first motor 81, the top of the guide frame 82 is connected with the second motor 83, the guide frame 82 is rotatably connected with the first screw rod 84, the top of the first screw rod 84 is connected with the output shaft of the second motor 83, the guide frame 82 is slidably connected with the first movable plate 85, the first movable plate 85 is in threaded connection with the first screw rod 84, the left side of the first movable plate 85 is connected with the electric liquid suction device 86, the right side of the first movable plate 85 is connected with the electric sucking disc 87, and the first movable plate 86, the electric liquid suction device 86 and the electric liquid suction device 87 are opened through the first motor 83 and the electric liquid suction device 87.
The testing mechanism 9 comprises a supporting frame 91, a third motor 92, a second screw rod 93 and a second movable plate 94, the middle part of the rear side of the top of the base 1 is connected with the supporting frame 91, the top of the supporting frame 91 is bolted with the third motor 92, the third motor 92 is opened, the conductivity tester 7 can be moved, the second screw rod 93 is rotatably connected inside the supporting frame 91, the top of the second screw rod 93 is connected with the output shaft of the third motor 92, the second screw rod 93 is connected with the second movable plate 94 in a threaded manner, the second movable plate 94 is slidably connected with the supporting frame 91, and the front part of the second movable plate 94 is connected with the conductivity tester 7.
The sterilization mechanism 10 comprises an annular rack 101, a sliding block 102, a fourth motor 103, a first gear 104 and a hydrogen peroxide sterilizer 105, as shown in fig. 9, the upper part of the inner side of the isolation cover 2 is connected with the annular rack 101, the inner side of the annular rack 101 is connected with the sliding block 102 in a sliding manner, the upper part of the sliding block 102 is connected with the fourth motor 103, the output shaft of the fourth motor 103 is connected with the first gear 104, the first gear 104 is meshed with the annular rack 101, the front side of the sliding block 102 is connected with the hydrogen peroxide sterilizer 105, and the inside of the isolation cover 2 can be sterilized by starting the hydrogen peroxide sterilizer 105.
The isolation mechanism 11 comprises a rotating rod 1101, a baffle 1102, an elastic piece 1103, a second gear 1104 and a toothed plate 1105, as shown in fig. 7 and 8, the outer sides of the tops of the first placing frame 5 and the second placing frame 6 are respectively connected with the rotating rod 1101 in a rotating mode, the baffle 1102 is respectively connected with the lower sides of the two rotating rods 1101, the baffle 1102 is reversely rotated to the upper sides of the liquid medicine pipe 15 and the transwell chamber 16, the liquid medicine pipe 15 and the transwell chamber 16 can be shielded, the elastic piece 1103 is connected between the baffle 1102 on the front side and the first placing frame 5, the elastic piece 1103 is a torsion spring, the elastic piece 1103 is also connected between the baffle 1102 on the rear side and the second placing frame 6, the upper sides of the two rotating rods 1101 are respectively connected with the second gear 1104 through a one-way clutch, the upper parts on the left side and the right sides of the tops of the guide frames 82 are respectively connected with the toothed plate 1105, and the toothed plate 1105 can be contacted with the second gear 1104 when rotating.
When people need to use the endothelial cell monolayer permeability detection experimental device, firstly, a polycarbonate film is placed in a transwell chamber 16, then a movable door 3 is pulled to be opened by rotation, a glass 14 is placed at the lower part of a supporting frame 91, the glass 14 is positioned under an electric liquid suction device 86, a baffle 1102 is pushed to rotate, an elastic piece 1103 is deformed, a liquid medicine pipe 15 filled with liquid medicine is placed in a second placing frame 6, then the transwell chamber 16 is placed on a first placing frame 5, the baffle 1102 is released, the elastic piece 1103 is restored to the original state, the elastic piece 1103 drives the baffle 1102 to reversely reset, the baffle 1102 is made to cover the liquid medicine tube 15 and the transwell chamber 16, then the movable door 3 is pushed to be reversely closed, the fourth motor 103 is started, the output shaft of the fourth motor 103 drives the first gear 104 to rotate, under the action of the annular rack 101, the first gear 104, the fourth motor 103 and the sliding block 102 move in an annular mode, then the hydrogen peroxide sterilizer 105 is started, the hydrogen peroxide sterilizer 105 is made to sterilize the inside of the isolation cover 2, after the sterilization of the inside of the isolation cover 2 is completed, the hydrogen peroxide sterilizer 105 and the fourth motor 103 are closed, after a period of time, the first motor 81 is started, the output shaft of the first motor 81 is controlled to rotate positively, so that the guide frame 82, the toothed plate 1105, the first movable plate 85, the electric liquid suction device 86 and the electric sucking disc 87 are driven to rotate positively, the electric liquid suction device 86 rotates to the position right above the liquid medicine tube 15, when the toothed plate 1105 at the rear side contacts with the second gear 1104 at the rear side, the toothed plate 1105 at the rear side drives the second gear 1104 at the rear side to rotate, so that the rotary rod 1101 at the rear side and the baffle 1102 at the rear side are driven to rotate, the elastic piece 1103 deforms, the baffle 1102 at the rear side does not block the liquid medicine tube 15 any more, then the output shaft of the first motor 81 is controlled to stop rotating positively, then the second motor 83 is started, the output shaft of the second motor 83 is controlled to rotate positively, so as to drive the first screw rod 84 to rotate positively, the first movable plate 85, the electric liquid suction device 86 and the electric sucking disc 87 are driven to move downwards, so that the electric liquid suction device 86 is inserted into the liquid medicine in the liquid medicine pipe 15, then the output shaft of the second motor 83 is controlled to stop rotating positively, then the electric liquid suction device 86 is controlled to be started, so that the electric liquid suction device 86 sucks the liquid medicine in the liquid medicine pipe 15, then the electric liquid suction device 86 is closed, then the output shaft of the second motor 83 is controlled to rotate reversely, so as to drive the first screw rod 84 to rotate positively, and the first movable plate 85, the electric liquid suction device 86 and the electric sucking disc 87 are driven to move upwards for resetting, so that the electric liquid suction device 86 is separated from the liquid medicine in the liquid medicine pipe 15, then the output shaft of the second motor 83 is controlled to stop reversing, then the output shaft of the first motor 81 is controlled to reversely reset, so that the guide frame 82, the toothed plate 1105, the first movable plate 85, the electric liquid suction device 86 and the electric sucking disc 87 are driven to reversely reset, the electric liquid suction device 86 is reversely rotated to the position right above the glass 14, the toothed plate 1105 at the rear side drives the second gear 1104 at the rear side to reversely reset, so that the rotary rod 1101 at the rear side and the baffle 1102 at the rear side are driven to reversely reset, the elastic piece 1103 is restored to the original state, the baffle 1102 at the rear side is enabled to block the liquid medicine pipe 15 again, when the rear toothed plate 1105 is separated from the rear second gear 1104, the rear rotating rod 1101 and the rear baffle 1102 stop reversing, then the output shaft of the first motor 81 is controlled to stop reversing, the output shaft of the second motor 83 is controlled to rotate forward again, the electric aspirator 86 and the electric suction cup 87 are moved downward, so that the electric aspirator 86 enters the glass 14, then the output shaft of the second motor 83 is controlled to stop rotating forward, the electric aspirator 86 is controlled to be opened again, so that the electric aspirator 86 ejects the liquid medicine into the glass 14, then the electric aspirator 86 is closed, the output shaft of the second motor 83 is controlled to rotate reversely again to enable the electric liquid suction device 86 and the electric sucking disc 87 to move upwards for resetting, so that one-time liquid medicine feeding is completed, then the output shaft of the first motor 81 is controlled to rotate forwardly again to drive the guide frame 82, the toothed plate 1105, the first movable plate 85, the electric liquid suction device 86 and the electric sucking disc 87 to rotate forwardly, the electric sucking disc 87 rotates to the position right above the transwell chamber 16, when the toothed plate 1105 at the front side contacts with the second gear 1104 at the front side, the toothed plate 1105 at the front side drives the second gear 1104 at the front side to rotate, so as to drive the rotary rod 1101 at the front side and the baffle 1102 at the front side to rotate, the elastic member 1103 is deformed to make the baffle 1102 at the front side no longer block the transwell chamber 16, then control the output shaft of the first motor 81 to stop rotating forward, then control the output shaft of the second motor 83 to rotate forward, thereby driving the first screw rod 84 to rotate forward, making the first movable plate 85, the electric aspirator 86 and the electric sucker 87 move downward, thereby making the electric sucker 87 contact with the transwell chamber 16, then control the output shaft of the second motor 83 to stop rotating forward, then control the electric sucker 87 to open, making the electric sucker 87 suck the transwell chamber 16, then control the output shaft of the second motor 83 to rotate reversely, thereby driving the first screw rod 84 to rotate positively, enabling the first movable plate 85, the electric liquid absorber 86 and the electric sucking disc 87 to move upwards and reset, enabling the electric sucking disc 87 to suck the transwell cell 16 to move upwards, controlling the output shaft of the second motor 83 to stop reversing, controlling the output shaft of the first motor 81 to reverse, driving the guide frame 82, the toothed plate 1105, the first movable plate 85, the electric liquid absorber 86 and the electric sucking disc 87 to reverse, enabling the toothed plate 1105 on the front side to drive the second gear 1104 on the front side to reverse and reset, driving the rotating rod 1101 on the front side and the baffle 1102 on the front side to reverse and reset, when the elastic member 1103 is restored, the front toothed plate 1105 is separated from the front second toothed plate 1104, the front rotary rod 1101 and the front baffle 1102 stop rotating reversely, and when the guide frame 82, the toothed plate 1105, the first movable plate 85, the electric suction device 86 and the electric suction cup 87 rotate reversely and reset, the electric suction cup 87 rotates reversely and is positioned right above the glass 14, the output shaft of the first motor 81 is controlled to stop rotating reversely, the output shaft of the second motor 83 is controlled to rotate reversely again, the electric suction device 86 and the electric suction cup 87 move downwards, so that the electric suction cup 87 drives the transwell chamber 16 to enter the glass 14, when the transwell chamber 16 contacts with the glass 14, the output shaft of the second motor 83 is controlled to stop rotating positively, then the electric sucking disc 87 is controlled to be closed, the electric sucking disc 87 releases the transwell chamber 16, the output shaft of the second motor 83 is controlled to rotate reversely again, the electric aspirator 86 and the electric sucking disc 87 are moved upwards to reset, the electric sucking disc 87 is separated from the transwell chamber 16, then the output shaft of the first motor 81 is controlled to rotate positively to reset, the guide frame 82, the toothed plate 1105, the first movable plate 85, the electric aspirator 86 and the electric sucking disc 87 are driven to rotate positively to reset, the electric liquid suction device 86 is positioned right above the glass 14 again, then the operation is repeated to finish the liquid medicine feeding once again, the electric liquid suction device 86 sprays the liquid medicine in the transwell chamber 16, then the first motor 81 and the second motor 83 are closed, the third motor 92 is opened, the output shaft of the third motor 92 is controlled to rotate positively, thereby driving the second screw rod 93 to rotate positively, the second movable plate 94 and the conductivity tester 7 are moved downwards, one end of the conductivity tester 7 is inserted into the liquid medicine in the glass 14, the other end of the conductivity tester 7 is inserted into the liquid medicine in the transwell chamber 16, then the third motor 92 is closed, then the conductivity tester 7 and the display 4 are started, the conductivity tester 7 starts to detect, the detected data is displayed on the display 4, then people can obtain the detection result by watching the display 4, after the conductivity tester 7 completes detection, the conductivity tester 7 and the display 4 are closed, the third motor 92 is started, the output shaft of the third motor 92 is controlled to rotate reversely, the second screw rod 93 is driven to rotate reversely, the second movable plate 94 and the conductivity tester 7 move upwards to reset, the third motor 92 is closed, the movable door 3 is pulled to rotate and open, then the glass cup 14 is taken out from the lower part of the supporting frame 91, the baffle 1102 is pushed to rotate, the elastic piece 1103 is deformed, then the liquid medicine pipe 15 filled with liquid medicine is taken out from the second placing frame 6, the baffle 1102 is loosened, the elastic piece 1103 is restored to the original state, the elastic piece 1103 drives the baffle 1102 to reversely reset, and then the movable door 3 is pushed to reversely close.
Example 2
On the basis of embodiment 1, the cleaning mechanism 12 is further included, the cleaning mechanism 12 comprises a supporting rod 1202 and wiping cotton 1203, an annular chute 1201 is formed in the outer side of the top of the base 1, the annular chute 1201 is located in the isolation cover 2, the supporting rod 1202 is welded to the lower portion of the rear side of the sliding block 102, the supporting rod 1202 slides in the annular chute 1201, the wiping cotton 1203 is connected to the rear side of the supporting rod 1202, and the wiping cotton 1203 can wipe the inner wall of the isolation cover 2 through movement of the wiping cotton 1203, and the wiping cotton 1203 is in contact with the inner wall of the isolation cover 2.
When the sliding block 102 moves in an annular mode, the sliding block 102 drives the supporting rod 1202 and the wiping cotton 1203 to move in an annular mode, the wiping cotton 1203 wipes the inner wall of the isolation cover 2, therefore people can watch the internal situation conveniently through the isolation cover 2, and when the sliding block 102 stops moving, the supporting rod 1202 and the wiping cotton 1203 stop moving.
The shielding mechanism 13 comprises a shielding shell 1301 and a rubber sleeve 1302, the shielding shell 1301 is welded on the upper portion of the inner side of the annular rack 101, the outer side of the shielding shell 1301 is connected with the isolation cover 2, the rubber sleeve 1302 is connected to the guide frame 82, the fourth motor 103 and the first screw rod 84 can be protected under the action of the shielding shell 1301 and the rubber sleeve 1302, and the left side and the right side of the first movable plate 85 penetrate through the rubber sleeve 1302.
When people use the endothelial cell monolayer permeability detection experimental device, under the action of the protective shell 1301, the fourth motor 103 can be protected, when the hydrogen peroxide sterilizer 105 is sterilizing, the rubber sleeve 1302 can protect the first screw rod 84, when the first movable plate 85 moves, the rubber sleeve 1302 deforms, and when the first movable plate 85 resets, the rubber sleeve 1302 returns to the original state.
Finally, it should be noted that: the foregoing description is only illustrative of the preferred embodiments of the present invention, and although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements or changes may be made without departing from the spirit and principles of the present invention.
Claims (7)
1. The utility model provides an endothelial cell monolayer permeability test experimental device, including base (1), cage (2), dodge gate (3) and conductivity tester (7), base (1) top is connected with cage (2), cage (2) front portion rotation is connected with dodge gate (3), conductivity tester (7) are used for experimental detection, a serial communication port, still include display (4), first rack (5), second rack (6), control mechanism (8), test mechanism (9), sterilization mechanism (10) and isolation mechanism (11), cage (2) front side upper portion is connected with display (4), base (1) top front side right part is connected with first rack (5), base (1) top rear side right part is connected with second rack (6), first rack (5) and second rack (6) are located the inside of cage (2), base (1) upside is equipped with control mechanism (8) that are used for automatic material preparation, base (1) top rear side middle part is equipped with and is used for making conductivity tester (7) remove conductivity tester (9) and install on conductivity tester (7) through display tester (7), the upper part of the inner side of the isolation cover (2) is provided with a sterilizing mechanism (10) for sterilizing, an isolation mechanism (11) for blocking an experimental instrument is arranged between the first placing frame (5) and the second placing frame (6), the isolation cover (2) and the movable door (3) are used for forming a closed space by placing the experimental instrument on the first placing frame (5) and the second placing frame (6), the experimental instrument is shielded by the isolation mechanism (11), the closed space is sterilized by the sterilizing mechanism (10), the experimental instrument and the liquid medicine are subjected to material preparation by the control mechanism (8), the electrical conductivity tester (7) is driven to move by the testing mechanism (9), the electrical conductivity tester (7) starts to detect, and the display (4) is used for displaying detection data;
The control mechanism (8) comprises a first motor (81), a guide frame (82), a second motor (83), a first screw rod (84), a first movable plate (85), an electric liquid suction device (86) and an electric sucking disc (87), wherein the first motor (81) is connected to the right side of the middle part of the upper side of the base (1), the guide frame (82) is rotatably connected to the right side of the middle part of the upper side of the base (1), the bottom of the guide frame (82) is connected to the output shaft of the first motor (81), the second motor (83) is connected to the top of the guide frame (82), the first screw rod (84) is rotatably connected to the output shaft of the second motor (83), the first movable plate (85) is connected to the first screw rod (84) in a sliding mode, the electric liquid suction device (86) is connected to the left side of the first movable plate (85), the electric sucking disc (87) is connected to the right side of the first movable plate (85), the first motor (81) and the first motor (84) and the second motor (83) are rotatably connected to the output shaft of the first motor (83), the first screw rod (84) and the electric liquid suction device (86) are driven by the first motor (81) and the second motor (83), and the electric liquid suction device (87) are automatically started, and the electric liquid suction device (87) is automatically started;
The testing mechanism (9) comprises a supporting frame (91), a third motor (92), a second screw rod (93) and a second movable plate (94), wherein the middle part of the rear side of the top of the base (1) is connected with the supporting frame (91), the top of the supporting frame (91) is connected with the third motor (92), the second screw rod (93) is rotatably connected inside the supporting frame (91), the top of the second screw rod (93) is connected with the output shaft of the third motor (92), the second screw rod (93) is connected with the second movable plate (94) in a threaded manner, the second movable plate (94) is connected with the supporting frame (91) in a sliding manner, the front part of the second movable plate (94) is connected with the conductivity tester (7), and the second screw rod (93) is driven to rotate through the third motor (92), so that the second movable plate (94) and the conductivity tester (7) are moved;
isolation mechanism (11) including bull stick (1101), baffle (1102), elastic component (1103), second gear (1104) and pinion rack (1105), first rack (5) and second rack (6) top outside all rotate and are connected with bull stick (1101), bull stick (1101) downside all is connected with baffle (1102), be connected with elastic component (1103) between baffle (1102) and first rack (5) of front side, also be connected with elastic component (1103) between baffle (1102) and second rack (6) of rear side, bull stick (1101) upside all is connected with second gear (1104) through one-way clutch, left side upper portion and top right side of leading truck (82) all are connected with pinion rack (1105), can contact with second gear (1104) when pinion rack (1105) rotate, place experimental apparatus through pushing baffle (1102) again, elastic component (1103) are used for baffle (1102) to reset, make baffle (1102) shelter from experimental apparatus.
2. The endothelial cell monolayer permeability test experimental device according to claim 1, wherein the sterilization mechanism (10) comprises an annular rack (101), a sliding block (102), a fourth motor (103), a first gear (104) and a hydrogen peroxide sterilizer (105), the upper portion of the inner side of the isolation cover (2) is connected with the annular rack (101), the sliding block (102) is connected to the inner side of the annular rack (101) in a sliding manner, the upper portion of the sliding block (102) is connected with the fourth motor (103), the first gear (104) is connected to an output shaft of the fourth motor (103), the first gear (104) is meshed with the annular rack (101), the front side of the sliding block (102) is connected with the hydrogen peroxide sterilizer (105), the first gear (104) is driven to rotate through the opening of the fourth motor (103), the sliding block (102) and the hydrogen peroxide sterilizer (105) are driven to move, and then the hydrogen peroxide sterilizer (105) is opened, so that the interior sterilization of the isolation cover (2) is realized.
3. The endothelial cell monolayer permeability test device according to claim 1, further comprising a cleaning mechanism (12) for wiping the isolation cover (2), wherein the cleaning mechanism (12) comprises a supporting rod (1202) and wiping cotton (1203), the annular chute (1201) is positioned in the isolation cover (2), the lower part of the rear side of the sliding block (102) is connected with the supporting rod (1202), the rear side of the supporting rod (1202) is connected with the wiping cotton (1203), the wiping cotton (1203) is contacted with the inner wall of the isolation cover (2), and the wiping cotton (1203) wipes the inner wall of the isolation cover (2) through the movement of the supporting rod (1202) and the wiping cotton (1203).
4. The endothelial cell monolayer permeability test device according to claim 3, further comprising a blocking mechanism (13) for protection, wherein the blocking mechanism (13) comprises a protective shell (1301) and a rubber sleeve (1302), the upper portion of the inner side of the annular rack (101) is connected with the protective shell (1301), the outer side of the protective shell (1301) is connected with a shielding cover (2), the guide frame (82) is connected with the rubber sleeve (1302), the left side and the right side of the first movable plate (85) penetrate through the rubber sleeve (1302), and protection of the fourth motor (103) and the first screw rod (84) is achieved through the protective shell (1301) and the rubber sleeve (1302).
5. An endothelial cell monolayer permeability test device according to claim 1, characterized in that the shielding (2) is of transparent material.
6. An endothelial cell monolayer permeability test device according to claim 1, wherein the resilient member (1103) is a torsion spring.
7. An endothelial cell monolayer permeability test device according to claim 3, wherein the outer side of the top of the base (1) is provided with an annular chute (1201), and the support rod (1202) slides in the annular chute (1201).
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