CN115028481A - Separation and extraction method of blue algae extract - Google Patents
Separation and extraction method of blue algae extract Download PDFInfo
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/59—Biological synthesis; Biological purification
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to a method for separating and extracting a blue algae extract, which can obviously enhance photosynthesis of green leaf plants and accumulate dry matters after being applied as a liquid fertilizer in the agricultural field. When blue algae is taken to drain water to 50% of solid content, 1-5ml of mixed biological enzyme is added into each kilogram of blue algae, enzymolysis is carried out for 10 hours at 60 ℃ to obtain primary enzymolysis liquid, 2L of 1-2% hydrogen peroxide aqueous solution is added into each kilogram of enzymolysis liquid, 100-square air is adopted for oxidation in each ton under the reaction conditions of 200 ℃ and 3.5Mpa pressure, and the oxidation reaction time is 2 hours. And squeezing and filtering the oxidized reactant to obtain clear liquid rich in micromolecular organic carbon source active substances. Experimental data show that the clear liquid can be independently used as a fertilizer for green-leaf plants and is also very suitable to be used as a production raw material of a liquid fertilizer.
Description
Technical Field
The invention relates to the technical field of biological extraction, in particular to a separation and extraction method of a blue algae extract.
Background
Blue-green algae, also known as cyanobacteria and blue-green algae, is the oldest known photoautotrophic organism on earth, and dates back to about 35 hundred million years. The blue algae photosynthetic system is similar to eukaryote, has chlorophyll a, a photosystem I and a photosystem II, and can reduce carbon dioxide into organic carbon and release oxygen by utilizing solar energy. The occurrence of blue-green algae obviously influences the evolution of the earth environment, and the aerobic change of the earth atmospheric environment, which is one of the most critical events in the process of the evolution of the earth, is initiated.
Besides the photosynthetic function, the blue algae also has the following unique physiological characteristics, so that the blue algae can be subjected to explosive propagation in the early severe environment of the earth, and finally, the atmospheric environment is changed.
1. Nitrogen fixation effect
The blue algae is the only algae capable of fixing nitrogen and can be divided into nitrogen-fixing blue algae and non-nitrogen-fixing blue algae according to whether the nitrogen-fixing effect exists or not. The nitrogen fixation function of the nitrogen-fixing blue algae is to reduce nitrogen molecules into nitrogen compounds by using nitrogen-fixing enzyme. Because the nitrogenase is inactivated under the aerobic condition, some nitrogen-fixing blue-green algae also form heteromorphic cells to separate the nitrogen fixing function from the photosynthesis.
2、CO 2 Concentration capacity
CO of blue algae 2 The concentration mechanism enables the cells to remove CO from the carboxyl body 2 The concentration is concentrated to the level that the carbon-fixing enzyme works efficiently. To date, 5 different inorganic carbon absorption systems have been found in cyanobacterial cells (2 for CO absorption 2 And 3 for absorbing bicarbonate). The carbon fixing rate of the cyanobacteria cells can be adjusted by combining the carbon absorption systems in different modes so as to adapt to the change of inorganic carbon in the environment, and the cyanobacteria cells can still effectively utilize the carbon source in the low-carbon environment.
3. Low light intensity adaptability
Sunlight entering the water is absorbed and scattered by some particles and organic matters in the water, and photosynthesis of producers in the water is limited. Compared with other phytoplankton, the cyanobacterial cell contains two main pigments of chlorophyll and carotenoid, and also contains pigments such as phycocyanin, phycoerythrin, allophycocyanin and the like, has a wider light-capturing spectrum, can fully capture and utilize a light source under low light intensity, and has stronger adaptability to the low light intensity.
4. Blue algae dormant body
The cyanobacteria dormant body is a self-protection strategy for the cyanobacteria to deal with the adverse environment. When the environment is not favorable for the growth of blue algae, algae cells sink into the sediment in the form of dormancy bodies; when the environmental condition is improved, the dormancy body is revived and grows and reproduces again. For example, in the case of a low temperature in winter, the algae cells mainly sink into the water bottom in the form of dormant bodies to live through the winter, and germinate again in the spring of the next year to become the seed source of the next year blue algae outbreak.
Researches show that the blue algae contains rich amino acids, proteins, polysaccharides, biological coenzymes, biological stimulants and other nutrient components, can be used for changing waste into valuable, and is applied to fertilizers of terrestrial plants, so that the problem of separating and extracting various nutrient substances from the blue algae becomes a solution of the scheme.
Disclosure of Invention
In order to search for a formula with higher safety and better separation effect, the invention provides a method for separating and extracting a blue algae extract, which can enable green leaf plants to obviously enhance photosynthesis and accumulate dry matters after being applied as a liquid fertilizer in the agricultural field.
A method for separating and extracting blue algae extract comprises the following steps,
s1: firstly, leaching out water from blue algae, adding 1-5ml of mixed biological enzyme into each kilogram of blue algae when the water content is reduced to 50% of solid content, and carrying out enzymolysis for 10 hours at 60 ℃ to obtain a primary enzymolysis liquid;
s2: pouring the preliminary enzymolysis liquid into a reaction vessel, adding 2L of 1-2 mass percent aqueous hydrogen peroxide into each kilogram of the preliminary enzymolysis liquid, and oxidizing each kilogram of the preliminary enzymolysis liquid by using 0.1 square air under the reaction conditions of 200 ℃ and 3.5Mpa pressure for 2 hours to obtain a reactant;
s3: and squeezing and filtering the oxidized reactant to obtain clear liquid rich in micromolecular organic carbon source active substances.
In a preferred embodiment of the invention, the mixed biological enzyme in S1 is one or more of cellulase, acid protease, lignin peroxidase, glucose oxidase, pectinase and L amino acid oxidase.
In a preferred embodiment of the present invention, the reaction vessel in S2 is a high temperature and high pressure reaction vessel.
In a preferred embodiment of the invention, the blue algae extract can be used as a fertilizer for green-leaf plants and can also be used as a raw material for producing liquid fertilizers.
The method for separating and extracting the blue algae extract has the following beneficial effects:
the invention adopts blue algae and applies bioengineering technology to prepare the blue algae into pollution-free green feed, thereby not only preventing and treating the flood and disaster of blue algae and other plants, but also improving the ecological environment, turning harm into good and changing waste into valuable, and after being applied as liquid fertilizer, the invention can ensure that the photosynthesis of green leaf plants can be obviously enhanced and dry matters can be accumulated.
Detailed Description
The invention discloses a separation and extraction method of a blue algae extract, which can be realized by appropriately improving process parameters by taking the contents of the method as reference by a person skilled in the art. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. The method for separating and extracting blue algae extract of the present invention has been described by way of preferred embodiments, and it will be apparent to those skilled in the art that the techniques of the present invention may be implemented and applied by modifying or appropriately changing or combining the methods and applications described herein without departing from the content, spirit and scope of the present invention. In order to make those skilled in the art better understand the technical solution of the present invention, the following detailed description of the present invention is provided with reference to specific embodiments.
Example 1: a separation and extraction method of a blue algae extract comprises the following steps:
firstly, a plurality of blue algae are taken to drain water, when the water content is reduced to 50 percent of solid content, 1ml of cellulase and acid protease are added into each kilogram of blue algae, and the blue algae is subjected to enzymolysis for 10 hours at the temperature of 60 ℃ to obtain a primary enzymolysis liquid; pouring the preliminary enzymatic hydrolysate into a high-temperature high-pressure reaction kettle, adding 2L of 1 mass percent aqueous hydrogen peroxide into each kilogram of the preliminary enzymatic hydrolysate, and oxidizing each kilogram of the preliminary enzymatic hydrolysate by using 0.1 square air under the reaction conditions of 200 ℃ and 3.5MPa, wherein the oxidation reaction time is 2 hours to obtain a reactant; and squeezing and filtering the reoxidized reactant to obtain clear liquid rich in micromolecular organic carbon source active substances.
Example 2: a method for separating and extracting a blue algae extract comprises the following steps:
firstly, leaching a plurality of blue algae to remove water, adding 2ml of cellulase, lignin peroxidase and glucose oxidase mixed biological enzyme into each kilogram of blue algae when the water content is reduced to 50 percent of solid content, and carrying out enzymolysis for 10 hours at the temperature of 60 ℃ to obtain a primary enzymolysis liquid; pouring the preliminary enzymatic hydrolysate into a high-temperature high-pressure reaction kettle, adding 2L of 1 mass percent aqueous hydrogen peroxide into each kilogram of the preliminary enzymatic hydrolysate, and oxidizing each kilogram of the preliminary enzymatic hydrolysate by using 0.1 square air under the reaction conditions of 200 ℃ and 3.5MPa, wherein the oxidation reaction time is 2 hours to obtain a reactant; and squeezing and filtering the reoxidized reactant to obtain clear liquid rich in micromolecular organic carbon source active substances.
Example 3: a separation and extraction method of a blue algae extract comprises the following steps:
firstly, leaching a plurality of blue algae to remove water, adding 3ml of mixed biological enzyme of cellulase, pectinase and glucose oxidase per kilogram when the water is reduced to 50 percent of solid content, and carrying out enzymolysis for 10 hours at the temperature of 60 ℃ to obtain a primary enzymolysis liquid; pouring the preliminary enzymolysis liquid into a high-temperature high-pressure reaction kettle, adding 2L of 2 mass percent aqueous hydrogen peroxide into each kilogram of the preliminary enzymolysis liquid, and oxidizing each kilogram of the preliminary enzymolysis liquid by adopting 0.1 square of air under the reaction conditions of 200 ℃ and 3.5MPa, wherein the oxidation reaction time is 2 hours to obtain a reactant; and squeezing and filtering the reoxidized reactant to obtain clear liquid rich in micromolecular organic carbon source active substances.
Example 4: a separation and extraction method of a blue algae extract comprises the following steps:
firstly, leaching a plurality of blue algae to remove water, adding 4ml of mixed biological enzyme of cellulase, pectinase, glucose oxidase and L-amino acid oxidase enzyme into each kilogram of blue algae when the water content is reduced to 50 percent of solid content, and carrying out enzymolysis for 10 hours at the temperature of 60 ℃ to obtain a primary enzymolysis liquid; pouring the preliminary enzymolysis liquid into a high-temperature high-pressure reaction kettle, adding 2L of 2 mass percent aqueous hydrogen peroxide into each kilogram of the preliminary enzymolysis liquid, and oxidizing each kilogram of the preliminary enzymolysis liquid by adopting 0.1 square of air under the reaction conditions of 200 ℃ and 3.5MPa, wherein the oxidation reaction time is 2 hours to obtain a reactant; and squeezing and filtering the reoxidized reactant to obtain clear liquid rich in micromolecular organic carbon source active substances.
Example 5: a separation and extraction method of a blue algae extract comprises the following steps:
firstly, leaching a plurality of blue algae to remove water, adding 5ml of cellulase, acid protease, pectinase, glucose oxidase and L-amino acid oxidase mixed biological enzyme into each kilogram of blue algae when the water content is reduced to 50 percent of solid content, and carrying out enzymolysis for 10 hours at the temperature of 60 ℃ to obtain a primary enzymolysis liquid; pouring the preliminary enzymolysis liquid into a high-temperature high-pressure reaction kettle, adding 2L of 2 mass percent aqueous hydrogen peroxide into each kilogram of the preliminary enzymolysis liquid, and oxidizing each kilogram of the preliminary enzymolysis liquid by adopting 0.1 square of air under the reaction conditions of 200 ℃ and 3.5MPa, wherein the oxidation reaction time is 2 hours to obtain a reactant; and squeezing and filtering the reoxidized reactant to obtain clear liquid rich in micromolecular organic carbon source active substances.
The clear liquid prepared in the above examples 1 to 5 can be used not only as a fertilizer for green leaf plants alone, but also as a raw material for producing liquid fertilizers.
The following are comparative examples using the blue algae extract obtained in example 5 as a field fertilizer:
the first field test:
crop: peanut
And (3) experimental design: the test was divided into 3 groups as follows:
example (b): spraying blue algae extracting solution
Comparative example 1: spray clear water control
Comparative example 2: spraying multifunctional foliage fertilizer for certain brand
And (3) test results: according to the fact that the blue algae extract in the embodiment is diluted and used as a fertilizer, tests are carried out in the field, and the result shows that the yield of peanuts can be remarkably improved by applying the blue algae extract, compared with other fertilization, the yield is improved by 11.43%, and compared with a clear water control, the yield is increased by 29.12%. The kernel yield is improved by 5.89% compared with other fertilization and is increased by 10.70% compared with clear water, which is shown in table 1.
TABLE 1 growth index of peanuts under different grouping treatment (statistical mean)
The blue algae extract disclosed by the invention is used as a fertilizer, has an influence on improving the peanut quality, can obviously improve the protein and fat content of peanut grains, reduces the content of soluble sugar, and improves the oleic acid-linoleic acid ratio (O/L), and is specifically shown in Table 2.
TABLE 2 quality index of peanut kernels under different grouping treatment (statistical mean)
And (2) field test II:
crop: wheat (Triticum aestivum L.)
And (3) experimental design: the test was divided into 3 groups as follows:
example (b): spraying blue algae extracting solution
Comparative example 1: spraying clear water contrast
Comparative example 2: spraying certain brand multifunctional foliage spray fertilizer
And (3) test results: the statistical data of various growth indexes of wheat show that the wheat using the blue algae extract as the fertilizer is obviously higher than other control groups in the indexes such as plant height, spike grain number, thousand grain weight and the like, and the embodiment also has better performance in dry matter accumulation and yield, and the specific data are shown in Table 3.
TABLE 3 fertility index of wheat under different grouping treatment (statistical mean)
And (3) field test III:
crop: tomato
And (3) experimental design: the test was divided into 3 groups as follows:
example (b): spraying blue algae extracting solution
Comparative example 1: spraying clear water contrast
Comparative example 2: spraying certain brand multifunctional foliage spray fertilizer
And (3) test results: when the blue algae extract in the embodiment is used as a fertilizer, the leaves are dark green, the branches and leaves are luxuriant, the growth is tidy, the stems are thick and strong, the flowers are more, the fruits are more, the yield is obviously increased, and the specific data are shown in table 4.
TABLE 4 tomato growth index (statistical mean) under different grouping treatment
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (4)
1. A separation and extraction method of blue algae extract is characterized in that the separation and extraction method comprises the following steps,
s1: firstly, leaching out water from blue algae, adding 1-5ml of mixed biological enzyme into each kilogram of blue algae when the water content is reduced to 50% of solid content, and carrying out enzymolysis for 10 hours at the temperature of 60 ℃ to obtain a primary enzymolysis liquid;
s2: pouring the preliminary enzymolysis liquid into a reaction vessel, adding 2L of 1-2 mass percent aqueous hydrogen peroxide into each kilogram of the preliminary enzymolysis liquid, and oxidizing each kilogram of the preliminary enzymolysis liquid by using 0.1 square air under the reaction conditions of 200 ℃ and 3.5Mpa pressure for 2 hours to obtain a reactant;
s3: and squeezing and filtering the oxidized reactant to obtain clear liquid rich in micromolecular organic carbon source active substances.
2. The method for separating and extracting a blue algae extract according to claim 1, wherein the mixed biological enzyme in S1 is one or more of cellulase, acid protease, lignin peroxidase, glucose oxidase, pectinase and L-amino acid oxidase.
3. The method for separating and extracting the blue algae extract according to claim 1, wherein the reaction vessel in the S2 is a high-temperature high-pressure reaction vessel.
4. The method for separating and extracting the blue algae extract according to any one of claims 1 to 3, wherein the blue algae extract can be used as a fertilizer for green-leaf plants and can also be used as a raw material for producing liquid fertilizers.
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