CN115007232B - Microfluidic chip and liquid drop in-situ blasting method based on Janus swimming microelectrodes - Google Patents
Microfluidic chip and liquid drop in-situ blasting method based on Janus swimming microelectrodes Download PDFInfo
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- CN115007232B CN115007232B CN202210747233.2A CN202210747233A CN115007232B CN 115007232 B CN115007232 B CN 115007232B CN 202210747233 A CN202210747233 A CN 202210747233A CN 115007232 B CN115007232 B CN 115007232B
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
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Abstract
The invention discloses a microfluidic chip and a droplet in-situ blasting method, which comprises a cover plate, a substrate, a PDMS channel, an inlet and an outlet; the inner sides of the cover plate and the substrate are respectively provided with a conductive layer used for connecting an external power supply; at least 1 Janus swimming microelectrode capable of freely moving in the cavity is arranged in the cavity; the Janus swimming microelectrode comprises a conductive part and a magnetic part which are combined together, the movement of the Janus swimming microelectrode in the cavity and the gesture thereof are controlled by an external magnetic field through the magnetic part, and the conduction of the Janus swimming microelectrode and the conductive layers of the cover plate and the substrate is realized through the conductive part, so that a local electric field is formed, and the liquid drop is blasted. The invention is suitable for the biomedical fields of targeted drug delivery, cell therapy, culture and the like.
Description
Technical Field
The invention relates to the field of cell biology such as targeted drug delivery, cell culture and the like, in particular to a microfluidic chip and a liquid drop in-situ blasting method based on Janus swimming microelectrodes.
Background
The droplet microfluidic technology is used as a novel microfluidic control form, can be used for preparing monodisperse micro droplets with controllable structure and components, and has a remarkable potential in flexible packaging and controllable release of active substances. Especially, the microcapsule with controllable size and internal structure of the template is made of double emulsion droplets or multiple emulsion droplets, and cross contamination between the encapsulation and the external environment can be effectively avoided due to the unique core-shell structure. Therefore, the microcapsule taking the liquid drop as the template is widely used as a transportation carrier for packaging, transporting and releasing various functional substances including medicines, cosmetic ingredients, cells and the like, and particularly, the microcapsule has excellent application prospect in the fields of biomedicine, chemical detection and the like by conveying the internal wrappage of the liquid drop to a designated area and realizing controllable release.
Currently, release of the wrap within the microdroplet is accomplished primarily by means of temperature control, chemical environment, mechanical stress response, and external electric field regulation. The release of substances inside the microcapsule regulated by temperature change is essentially achieved by utilizing the melting property of the temperature response material at a specific temperature, and is mainly aimed at isothermal sensitive materials such as lipids or hydrocarbon compounds (glycerin fatty acid, paraffin oil and eicosane). The chemical environment change can also cause the micro-droplet shell to undergo chemical reaction and be rapidly degraded, but the method also has special requirements on droplet shell structure materials, such as pH value sensitive materials, plastic response materials and the like, which means that the method can only release specific samples, thereby limiting the application range of the method. Mechanically stress-regulated droplet release requires a hard and brittle shell and complex channel structure, correspondingly limiting droplet size and internal active content. The electric control drop release is a drop rupture mode which does not depend on an external environment, the drop rupture mode can be flexibly regulated and controlled according to the external medium environment and the inner core substance, the controllability is strong, but complicated electrode structure design is needed if the release of active substances in drops in controllable directions and positions is to be realized.
In summary, the above-mentioned droplet release methods cannot release the core material of the droplet at any position and in any direction on the surface of the droplet, i.e., cannot realize in-situ cracking at any position on the surface of the droplet, and are lacking in flexibility. The random positions on the surface of the liquid drop are blasted, so that substances in the liquid drop are released to the designated positions according to the needs, and the targeted delivery of the medicine is facilitated.
Disclosure of Invention
The invention aims to provide a liquid drop in-situ blasting method based on Janus swimming microelectrodes and a microfluidic chip, which utilize the swimming microelectrodes to manufacture a strong local electric field at any position near liquid drops, blast a liquid drop shell at the position through the stronger local electric field, aim to realize the electric rupture at any position on the liquid drops, enable medicines in the liquid drops to be released along any direction, and provide theoretical and technical support for targeted drug delivery and cell medicine treatment.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the microfluidic chip comprises a plate-shaped cover plate, a substrate, a PDMS (polydimethylsiloxane) channel arranged between the cover plate and the substrate, a closed cavity formed between the cover plate, the substrate and the PDMS channel, and an inlet and an outlet communicated with the cavity; the inlet and the outlet are both arranged on the cover plate or the substrate, or the inlet and the outlet are respectively arranged on the cover plate and the substrate; the inner sides of the cover plate and the substrate are respectively provided with a conductive layer used for connecting an external power supply; at least 1 Janus swimming microelectrode capable of freely moving in the cavity is arranged in the cavity; the Janus swimming microelectrode comprises a conductive part and a magnetic part which are combined together, the movement and the gesture of the Janus swimming microelectrode in the cavity are controlled by an external magnetic field through the magnetic part, and the conduction of the Janus swimming microelectrode and the conductive layers of the cover plate and the substrate is realized through the conductive part, so that a local electric field is formed.
Preferably, the cover plate and the substrate are both made of conductive glass with an indium tin oxide coating.
Preferably, the distance between the cover plate and the substrate is 1.0mm; the Janus swimming microelectrode is spherical with the radius of 300 mu m or rod-shaped with the cross section radius of 300 mu m.
Preferably, the volume ratio of the conductive part to the magnetic part of the Janus swimming microelectrode is 1:1.
Preferably, the microfluidic chip is processed by the following process:
1) A channel mould with a linear cavity is obtained through numerical control machine tool processing, and the channel mould is stuck on the surface of the cleaned glass by using shadowless glue; cutting conductive glass with an indium tin oxide coating to manufacture the cover plate and the substrate; the cover plate is provided with an inlet and an outlet through drilling;
2) The polydimethyl siloxane and the curing agent are mixed according to the proportion of 10:1, pouring the mixture on the surface of smooth glass, and sticking a PVA film on the surface of the mixture after heating and curing to serve as a pressing plate;
3) The polydimethyl siloxane and the curing agent are mixed according to the proportion of 10:1, pouring the mixture on glass adhered with the channel mold in a closed processing device to cover the inside and outside of the cavity, and vacuumizing the processing device to remove bubbles in uncured polydimethylsiloxane; pressing the prepared pressing plate on uncured polydimethylsiloxane and pressing the pressing plate to the bottom with force; taking away the channel mold to obtain an uncured PDMS channel in a shape of a Chinese character 'kou'; then placing the obtained uncured PDMS channel and a pressing plate together in an oven for curing;
4) Cleaning one side of the opening of the cured PDMS channel and one side of the substrate coated with the indium tin oxide coating, putting the cleaned side into a plasma machine chamber, vacuumizing to enable the substrate to be in a vacuum environment, subsequently introducing oxygen as excitation gas, carrying out plasma treatment for 35 seconds under the action of a high-frequency electric field, taking out the substrate, and bonding the substrate to obtain a semi-finished product;
5) Putting the bonded semi-finished product into water, and dissolving the PVA film on the pressing plate to separate the pressing plate and the semi-finished product;
6) And carrying out plasma treatment on one side of the cover plate coated with the indium tin oxide coating and one side of the semi-finished product separated from the pressing plate, and bonding the cover plate and the semi-finished product to obtain the microfluidic chip.
Preferably, the Janus swimming microelectrode is processed by the following steps:
1) The preparation method comprises the following steps: mixing metal silver powder and water to prepare a conductive raw material; mixing magnetic ferroferric oxide powder with photo-curing adhesive powder to prepare magnetic raw materials;
2) Shaping; simultaneously pumping the conductive raw material and the magnetic raw material into a preparation tool, wherein the preparation tool comprises two inlet channels for the conductive raw material and the magnetic raw material to enter, a converging channel communicated with the two inlet channels, and a cutting hole penetrating through the converging channel; after the conductive raw material and the magnetic raw material are converged and enter a converging channel, cutting oil is periodically introduced into the cutting hole so as to penetrate through the converging channel, and therefore the conductive raw material and the magnetic raw material flowing in the converging channel are subjected to punching cutting so as to form blanks;
4) Curing, namely irradiating the blank by ultraviolet rays to cure the photo-curing adhesive in the blank to obtain Janus particles;
5) And heating, namely performing high-temperature heating treatment on the Janus particles to remove water in the Janus particles, and fusing silver powder in the Janus particles to obtain the Janus swimming microelectrode.
The invention also provides a liquid drop in-situ blasting method based on the Janus swimming microelectrode, which comprises the micro-fluidic chip and comprises the following steps of:
1) Hydrophilic treatment: cleaning and drying the inner cavity of the microfluidic chip, placing the microfluidic chip into a cavity of a plasma machine, performing plasma treatment on the inner surface of the cavity by using oxygen as excitation gas under the action of an electric field, and introducing hydrophilic-OH groups on the surface of the cavity to change the inner surface of the cavity into a hydrophilic surface so as to prevent the Janus swimming microelectrode from being adhered to the conductive layer of the cover plate or the substrate;
2) Filling KCL solution: preparing KCL solution with conductivity of 0.2S/m, and filling the KCL solution into a cavity of the microfluidic chip;
2) Preparing water-in-oil double emulsion drops; preparing a polystyrene particle of 500nm as an active substance model, coating the active substance model into an oil shell by utilizing a liquid drop microfluidic technology to form water-in-oil double emulsion liquid drops, and conveying the Janus swimming microelectrode and the water-in-oil double emulsion liquid drops into a cavity of the microfluidic chip to suspend the Janus swimming microelectrode and the water-in-oil double emulsion liquid drops in a KCL solution;
3) The conducting layer of the substrate is used as a grounding electrode, and the conducting layer of the cover plate is connected with the positive electrode of the output end of the signal amplifier;
4) The Janus swimming microelectrode is pulled by an electromagnet to swim in a cavity of the microfluidic chip, and a water-in-oil double-emulsion droplet is found and is close to the Janus swimming microelectrode under a microscope, so that a conductive part of the Janus swimming microelectrode contacts the water-in-oil double-emulsion droplet;
5) The signal generator is regulated to output proper voltage amplitude and electric signal frequency so as to generate a local strong electric field on the conductive part of the Janus swimming microelectrode, thereby being used for blasting water-in-oil double emulsion drops;
6) Repeating the above steps to obtain the optimal voltage amplitude and electrical signal frequency.
The beneficial effects of the invention are as follows:
the Janus swimming microelectrode is pulled by an external magnet to swim in the cavity and position to the position where the liquid drop needs to be blasted, and then the blasting of the liquid drop and the release of internal substances are controlled by adjusting external voltage and frequency signals. The magnetic field is used for regulating and controlling and randomly changing the contact position of the Janus swimming microelectrode and the micro-droplet so as to realize the blasting of any position on the surface of the droplet, and the gap of the technology in the current droplet blasting can be filled. The invention is suitable for the biomedical fields of targeted drug delivery, cell therapy, culture and the like.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic exploded view of the present invention;
FIG. 3 is a schematic structural diagram of a Janus swimming microelectrode;
fig. 4 is a schematic diagram of a process of manufacturing a microfluidic chip;
fig. 5 is a schematic diagram of a process for manufacturing a Janus swimming microelectrode.
Detailed Description
The technical scheme of the patent is further described in detail below with reference to the specific embodiments.
In the description of the present invention, it should be noted that the positional or positional relationship indicated by the terms such as "inner", "outer", "upper", "lower", "horizontal", etc. are based on the positional or positional relationship shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or element in question must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.
As shown in fig. 1 to 5, a microfluidic chip of the present invention includes a plate-shaped cover plate 1, a substrate 6, and a PDMS channel 4 (PDMS polydimethylsiloxane) disposed between the cover plate 1 and the substrate 6. A closed cavity is formed among the cover plate 1, the substrate 6 and the PDMS channel 4, and the device also comprises an inlet 2 and an outlet 3 which are communicated with the cavity; the inlet 2 and the outlet 3 are both provided on the cover plate 1. The cover plate 1 and the substrate 6 are each made of conductive glass with an indium tin oxide coating, and the indium tin oxide coating on the inner sides of the cover plate 1 and the substrate 6 forms a conductive layer for connecting an external power source.
At least 1 Janus swimming microelectrode 5 which can freely move in the cavity is arranged in the cavity; the Janus swimming microelectrode 5 comprises a conductive part 8 and a magnetic part 7 which are combined together, the movement of the Janus swimming microelectrode 5 in the cavity and the gesture thereof are controlled by an external magnetic field through the magnetic part 7, and the conduction of the Janus swimming microelectrode 5 with the conductive layers of the cover plate 1 and the substrate 6 is realized through the conductive part 8, so that a local electric field is formed.
Further, the spacing between the cover plate 1 and the base 6 is 1.0mm; the Janus swimming microelectrode 5 is spherical with a radius of 300 μm or rod-shaped with a cross-sectional radius of 300 μm. With the above-mentioned size structure, the conductive liquid in the cavity can be conducted through the conductive portion 8 of the Janus floating microelectrode 5 after the conductive layer is electrified.
The volume ratio of the conductive part 8 and the magnetic part 7 of the Janus swimming microelectrode 5 is 1:1.
The microfluidic chip is processed by the following processes:
1) A channel mould (not shown) with a linear cavity is obtained through numerical control machine tool processing, and the channel mould is stuck on the surface of the cleaned glass by using shadowless glue; cutting conductive glass with an indium tin oxide coating to form a cover plate 1 and a substrate 6; the cover plate 1 is provided with an inlet 2 and an outlet 3 through drilling;
2) The polydimethyl siloxane and the curing agent are mixed according to the proportion of 10:1, pouring the mixture on the surface of smooth glass, and sticking a PVA film (not shown) on the surface of the glass after heating and curing to serve as a pressing plate;
3) The polydimethyl siloxane and the curing agent are mixed according to the proportion of 10:1, pouring the mixture on glass adhered with a channel mold in a closed processing device (not shown) to cover the inside and the outside of a cavity, and vacuumizing the processing device to remove bubbles in uncured polydimethylsiloxane; pressing the prepared pressing plate on uncured polydimethylsiloxane, pressing the uncured polydimethylsiloxane to the bottom with force, and extruding and forming; taking away the channel mold to obtain an uncured PDMS channel 4 in a shape of a Chinese character 'kou'; then the obtained uncured PDMS channel 4 and the pressing plate are put together in an oven to be cured;
4) Cleaning one side of the opening of the cured PDMS channel and one side of the substrate coated with the indium tin oxide coating, putting the cleaned side into a plasma machine chamber, vacuumizing to enable the substrate to be in a vacuum environment, subsequently introducing oxygen as excitation gas, carrying out plasma treatment for 35 seconds under the action of a high-frequency electric field, taking out the substrate, and bonding the substrate to obtain a semi-finished product;
5) Placing the bonded semi-finished product into water, and dissolving a PVA film (PVA polyvinyl alcohol) on the pressing plate to separate the pressing plate and the semi-finished product;
6) And carrying out plasma treatment on the side of the cover plate 1 coated with the indium tin oxide coating and the side of the semi-finished product separated from the pressing plate, and bonding the cover plate and the semi-finished product to obtain the microfluidic chip.
The Janus swimming microelectrode 5 is manufactured by the following steps:
1) The preparation method comprises the following steps: mixing silver powder with water to prepare a conductive raw material 13; mixing magnetic ferroferric oxide powder and photo-curing adhesive powder to prepare a magnetic raw material 14;
2) Shaping; simultaneously pumping the conductive raw material 13 and the magnetic raw material 14 into a preparation tool 15, wherein the preparation tool 15 comprises two inlet channels 16 for the conductive raw material 13 and the magnetic raw material 14 to enter, a converging channel 17 communicated with the two inlet channels 16, and a cutting hole 18 penetrating through the converging channel 17; after the conductive raw material 13 and the magnetic raw material 14 are converged into the converging channel 17, cutting oil is periodically introduced into the cutting hole 18 so as to penetrate through the converging channel 17, so that the conductive raw material 13 and the magnetic raw material 14 flowing in the converging channel are subjected to punching cutting to form blanks; the blank is moved further along the converging channel 17 by cutting oil hydrodynamically. The device can obtain the required structure of the blank by adjusting the flow rate of the two inlet channels 16.
4) Curing, wherein ultraviolet light is emitted from an ultraviolet lamp 19 at the tail part of the converging channel 17 to irradiate the blank, so that a photo-curing adhesive (for example, ETPTA ethoxylated trimethylolpropane triacrylate) in the blank is cured to obtain Janus particles;
5) And heating, namely performing high-temperature heating treatment on the Janus particles to remove water in the Janus particles and fuse silver powder in the Janus particles to obtain the Janus swimming microelectrode 5.
The invention also provides a liquid drop in-situ blasting method based on the Janus swimming microelectrode 5, which comprises a microfluidic chip, and comprises the following steps:
1) Hydrophilic treatment: cleaning and drying the inner cavity of the microfluidic chip, placing the microfluidic chip into a cavity of a plasma machine, performing plasma treatment on the inner surface of the cavity by using oxygen as excitation gas under the action of an electric field, and introducing hydrophilic-OH groups on the surface of the cavity to change the inner surface of the cavity into a hydrophilic surface so as to prevent Janus floating microelectrodes 5 from being adhered to a conductive layer of a cover plate 1 or a substrate 6;
2) Filling KCL solution: preparing KCL solution with conductivity of 0.2S/m, and filling the KCL solution into a cavity of the microfluidic chip;
2) Preparing water-in-oil double emulsion drops; preparing a polystyrene particle of 500nm as an active substance model, coating the active substance model into an oil shell by utilizing a liquid drop microfluidic technology to form water-in-oil double-emulsion liquid drops, and dripping the Janus swimming microelectrode 5 and the water-in-oil double-emulsion liquid drops into a cavity of a microfluidic chip to suspend the Janus swimming microelectrode in KCL solution;
3) The signal generator is connected, the output end of the signal generator is connected with the input end of the signal amplifier, the conducting layer of the substrate 6 is used as a grounding electrode, and the conducting layer of the cover plate 1 is connected with the positive electrode of the output end of the signal amplifier;
4) The electromagnet is utilized to pull the Janus swimming microelectrode 5 to move in the cavity of the microfluidic chip, and the water-in-oil double-emulsion liquid drops are found and are close to the water-in-oil double-emulsion liquid drops under a microscope, so that the conductive part 8 of the Janus swimming microelectrode 5 is contacted with the water-in-oil double-emulsion liquid drops;
5) The signal generator is regulated to output proper voltage amplitude and electric signal frequency so as to generate a local strong electric field on the conductive part 8 of the Janus swimming microelectrode 5, thereby being used for blasting the water-in-oil double emulsion liquid drops and releasing the internal active substances;
6) Repeating the above steps to obtain the optimal voltage amplitude and electrical signal frequency.
The foregoing description is only illustrative of the present invention and is not intended to limit the scope of the invention, and all equivalent structures or equivalent processes or direct or indirect application in other related technical fields are included in the scope of the present invention.
Claims (7)
1. A microfluidic chip, characterized in that: the device comprises a plate-shaped cover plate, a substrate, a PDMS channel arranged between the cover plate and the substrate, a closed cavity formed among the cover plate, the substrate and the PDMS channel, and an inlet and an outlet communicated with the cavity; the inlet and the outlet are both arranged on the cover plate or the substrate, or the inlet and the outlet are respectively arranged on the cover plate and the substrate; the inner sides of the cover plate and the substrate are respectively provided with a conductive layer used for connecting an external power supply; at least 1 Janus swimming microelectrode capable of freely moving in the cavity is arranged in the cavity; the Janus swimming microelectrode comprises a conductive part and a magnetic part which are combined together, the movement and the gesture of the Janus swimming microelectrode in the cavity are controlled by an external magnetic field through the magnetic part, and the conduction of the Janus swimming microelectrode and the conductive layers of the cover plate and the substrate is realized through the conductive part, so that a local electric field is formed.
2. The microfluidic chip of claim 1, wherein: the cover plate and the substrate are both made of conductive glass with an indium tin oxide coating.
3. The microfluidic chip of claim 1, wherein: the distance between the cover plate and the substrate is 1.0mm; the Janus swimming microelectrode is spherical with the radius of 300 mu m or rod-shaped with the cross section radius of 300 mu m.
4. A microfluidic chip according to claim 3, wherein: the volume ratio of the conductive part to the magnetic part of the Janus swimming microelectrode is 1:1.
5. The microfluidic chip of claim 2, wherein the microfluidic chip is fabricated by the process of:
1) A channel mould with a linear cavity is obtained through numerical control machine tool processing, and the channel mould is stuck on the surface of the cleaned glass by using shadowless glue; cutting conductive glass with an indium tin oxide coating to manufacture the cover plate and the substrate; the cover plate is provided with an inlet and an outlet through drilling;
2) The polydimethyl siloxane and the curing agent are mixed according to the proportion of 10:1, pouring the mixture on the surface of smooth glass, and sticking a PVA film on the surface of the mixture after heating and curing to serve as a pressing plate;
3) The polydimethyl siloxane and the curing agent are mixed according to the proportion of 10:1, pouring the mixture on glass adhered with the channel mold in a closed processing device to cover the inside and outside of the cavity, and vacuumizing the processing device to remove bubbles in uncured polydimethylsiloxane; pressing the prepared pressing plate on uncured polydimethylsiloxane and pressing the pressing plate to the bottom with force; taking away the channel mold to obtain an uncured PDMS channel in a shape of a Chinese character 'kou'; then placing the obtained uncured PDMS channel and a pressing plate together in an oven for curing;
4) Cleaning one side of the opening of the cured PDMS channel and one side of the substrate coated with the indium tin oxide coating, putting the cleaned side into a plasma machine chamber, vacuumizing to enable the substrate to be in a vacuum environment, subsequently introducing oxygen as excitation gas, and carrying out plasma treatment under the action of a high-frequency electric field for 35 seconds to enable a large number of free radicals to be generated on the surface of the material, taking out the material, and bonding the material to obtain a semi-finished product;
5) Putting the bonded semi-finished product into water, and dissolving the PVA film on the pressing plate to separate the pressing plate and the semi-finished product;
6) And carrying out plasma treatment on one side of the cover plate coated with the indium tin oxide coating and one side of the semi-finished product separated from the pressing plate, and bonding the cover plate and the semi-finished product to obtain the microfluidic chip.
6. The microfluidic chip according to claim 4, wherein the Janus swimming microelectrode is processed by the steps of:
1) The preparation method comprises the following steps: mixing metal silver powder and water to prepare a conductive raw material; mixing magnetic ferroferric oxide powder with photo-curing adhesive powder to prepare magnetic raw materials;
2) Shaping; simultaneously pumping the conductive raw material and the magnetic raw material into a preparation tool, wherein the preparation tool comprises two inlet channels for the conductive raw material and the magnetic raw material to enter, a converging channel communicated with the two inlet channels, and a cutting hole penetrating through the converging channel; after the conductive raw material and the magnetic raw material are converged and enter a converging channel, cutting oil is periodically introduced into the cutting hole so as to penetrate through the converging channel, and therefore the conductive raw material and the magnetic raw material flowing in the converging channel are subjected to punching cutting so as to form blanks;
4) Curing, namely irradiating the blank by ultraviolet rays to cure the photo-curing adhesive in the blank to obtain Janus particles;
5) And heating, namely performing high-temperature heating treatment on the Janus particles to remove water in the Janus particles, and fusing silver powder in the Janus particles to obtain the Janus swimming microelectrode.
7. A method for in-situ blasting of droplets based on Janus travelling microelectrodes, comprising the microfluidic chip according to one of claims 1 to 6, characterized in that it comprises the following steps:
1) Hydrophilic treatment: cleaning and drying the inner cavity of the microfluidic chip, placing the microfluidic chip into a cavity of a plasma machine, performing plasma treatment on the inner surface of the cavity by using oxygen as excitation gas under the action of an electric field, and introducing hydrophilic-OH groups on the surface of the cavity to change the inner surface of the cavity into a hydrophilic surface so as to prevent the Janus swimming microelectrode from being adhered to the conductive layer of the cover plate or the substrate;
2) Filling KCL solution: preparing KCL solution with conductivity of 0.2S/m, and filling the KCL solution into a cavity of the microfluidic chip;
3) Preparing water-in-oil double emulsion drops; preparing 500nm polystyrene particles as an active material model, coating the active material model into an oil shell by utilizing a liquid drop microfluidic technology to form water-in-oil double-emulsion liquid drops, and conveying the Janus swimming microelectrodes and the water-in-oil double-emulsion liquid drops into a cavity of the microfluidic chip to suspend the Janus swimming microelectrodes and the water-in-oil double-emulsion liquid drops in a KCL solution;
4) The conducting layer of the substrate is used as a grounding electrode, and the conducting layer of the cover plate is connected with the positive electrode of the output end of the signal amplifier;
5) The Janus swimming microelectrode is pulled by an electromagnet to swim in a cavity of the microfluidic chip, and a water-in-oil double-emulsion droplet is found and is close to the Janus swimming microelectrode under a microscope, so that a conductive part of the Janus swimming microelectrode contacts the water-in-oil double-emulsion droplet;
6) The signal generator is regulated to output proper voltage amplitude and electric signal frequency so as to generate a local strong electric field on the conductive part of the Janus swimming microelectrode, thereby being used for blasting water-in-oil double emulsion drops;
7) Repeating the above steps to obtain the optimal voltage amplitude and electrical signal frequency.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210747233.2A CN115007232B (en) | 2022-06-28 | 2022-06-28 | Microfluidic chip and liquid drop in-situ blasting method based on Janus swimming microelectrodes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210747233.2A CN115007232B (en) | 2022-06-28 | 2022-06-28 | Microfluidic chip and liquid drop in-situ blasting method based on Janus swimming microelectrodes |
Publications (2)
| Publication Number | Publication Date |
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| CN115007232A CN115007232A (en) | 2022-09-06 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2020188563A1 (en) * | 2019-03-17 | 2020-09-24 | Technion Research & Development Foundation Limited | System and method for manipulating objects in a fluid |
| CN112080392A (en) * | 2020-09-21 | 2020-12-15 | 长春理工大学 | Three-dimensional dielectrophoresis micro-fluidic chip for separating circulating tumor cells at high flux |
| CN112808335A (en) * | 2021-01-21 | 2021-05-18 | 中国科学技术大学 | Preparation method of micro-fluidic chip for multi-parameter detection of water body |
| CN114166893A (en) * | 2021-12-09 | 2022-03-11 | 中国计量大学 | Method for detecting virus based on microfluidic impedance method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020188563A1 (en) * | 2019-03-17 | 2020-09-24 | Technion Research & Development Foundation Limited | System and method for manipulating objects in a fluid |
| CN112080392A (en) * | 2020-09-21 | 2020-12-15 | 长春理工大学 | Three-dimensional dielectrophoresis micro-fluidic chip for separating circulating tumor cells at high flux |
| CN112808335A (en) * | 2021-01-21 | 2021-05-18 | 中国科学技术大学 | Preparation method of micro-fluidic chip for multi-parameter detection of water body |
| CN114166893A (en) * | 2021-12-09 | 2022-03-11 | 中国计量大学 | Method for detecting virus based on microfluidic impedance method |
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