CN114907161B - Organic fertilizer containing biocontrol microbial inoculum and preparation method thereof - Google Patents
Organic fertilizer containing biocontrol microbial inoculum and preparation method thereof Download PDFInfo
- Publication number
- CN114907161B CN114907161B CN202210791717.7A CN202210791717A CN114907161B CN 114907161 B CN114907161 B CN 114907161B CN 202210791717 A CN202210791717 A CN 202210791717A CN 114907161 B CN114907161 B CN 114907161B
- Authority
- CN
- China
- Prior art keywords
- parts
- mixing
- organic fertilizer
- zeolite
- sodium hydroxide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Soil Sciences (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses an organic fertilizer containing a biocontrol microbial inoculum and a preparation method thereof, belonging to the technical field of organic fertilizers and microbial fertilizers. According to the invention, wormcast, straw, bean pulp, humic acid and the like are used as raw materials, and aspergillus polyvidus, pseudomonas paraflavus and bacillus subtilis are screened as active microorganisms to prepare the bio-organic fertilizer, so that necessary nutrients can be provided for plant growth, the content of effective nutrients and organic matters can be increased, the soil structure can be improved, the soil microbial activity can be increased, the soil fertility can be further improved, the crop growth can be promoted, the use of inorganic chemical fertilizers can be replaced, the toxic effect of the inorganic chemical fertilizers on soil can be reduced, and the economic benefit and the social benefit are remarkable.
Description
Technical Field
The invention belongs to the technical field of organic fertilizers and microbial fertilizers, and particularly relates to an organic fertilizer containing a biocontrol microbial inoculum and a preparation method thereof.
Background
The long-term excessive application of the chemical fertilizer causes a series of problems, such as soil hardening and acidification, organic matter content reduction, biological activity reduction, poor fruit and vegetable quality and the like, and the important measures for turning the adverse situations are realized by reducing the application amount of the chemical fertilizer and matching organic substitution in order to promote the sustainable development of agriculture. The biological organic fertilizer is prepared by composting and composting livestock and poultry manure, crop straws and other wastes and compounding the composted wastes with functional microorganisms. The biological organic fertilizer can increase soil fertility, purify and repair soil, and play an important role in promoting plant growth, reducing plant morbidity, increasing yield, improving crop quality and the like.
The bio-organic fertilizer comprises the following action mechanisms: after the biological organic fertilizer is applied to soil, functional bacteria in the fertilizer improve the soil fertility through various metabolic activities. For example, by applying a fertilizer containing nitrogen-fixing microorganisms, nitrogen-fixing bacteria in the fertilizer can reduce nitrogen which cannot be directly absorbed and utilized by plants in the air into ammonia through a special biological nitrogen-fixing effect, and the ammonia can be absorbed and utilized by the plants after being further converted into other nitrogen with different forms; applying a fertilizer containing phosphate-solubilizing and potassium-solubilizing microorganisms, wherein the microorganisms can decompose and convert insoluble or organic nutrients, such as tricalcium phosphate, aluminum phosphate, iron phosphate, organic phosphorus and the like, which cannot be directly or rapidly absorbed and utilized by plants in soil into nutrients which can be directly absorbed and utilized by the plants. (2) Functional bacteria in the biological organic fertilizer can secrete physiological active substances such as auxin (indoleacetic acid, IAA) and the like to promote plant growth, and meanwhile, when the functional bacteria propagate in plant root systems to form dominant species, the functional bacteria can also inhibit the growth and propagation of other pathogenic bacteria, so that the probability of infecting the plant root systems is reduced. (3) The biological organic fertilizer not only contains N, P and K, secondary elements (Ca, mg, S and the like) and trace elements (Fe, mn, cu and the like) for plants to absorb and utilize, but also contains organic components such as amino acid, protein, sugar, fat and the like, and the components can help the functional bacteria in the fertilizer to colonize and exert functions.
The application of the bio-organic fertilizer in China is still in the initial stage at present, the research is not deep, the yield is often reduced to some extent after the manufactured bio-organic fertilizer is applied independently compared with the single application of inorganic fertilizer and organic fertilizer, the current yield is influenced mainly because the nutrient of the bio-organic fertilizer is released slowly and can not be provided for the crops in time when the bio-organic fertilizer is applied independently, and therefore, the application of the bio-organic fertilizer is limited, and the bio-organic fertilizer is required to be matched with the inorganic fertilizer or the organic fertilizer even if the bio-organic fertilizer is used, but the promotion of the growth and the quality of the crops is not obvious. And the fertilizer efficiency time is short, the fertilizer needs to be applied repeatedly for many times, in practice, the application rate is not high enough, and the improvement effect on the soil environment is relatively common.
Disclosure of Invention
Aiming at the problems of poor fertilizer effect and single function of the biological organic fertilizer in the prior art, the invention provides the high-efficiency biological organic fertilizer, which improves the physical and chemical properties and biological activity of soil, fundamentally solves the problems, provides continuous and effective nutrition for crops, and improves the yield and quality of the crops.
In order to achieve the technical purpose, the technical scheme adopted by the invention is as follows:
an organic fertilizer containing a bio-control microbial inoculum is prepared from the following raw materials in parts by weight: 50-90 parts of straw, 50-80 parts of wormcast, 20-50 parts of bean pulp, 10-40 parts of corn flour, 10-15 parts of humic acid, 5-15 parts of microbial agent, 9-19 parts of porous zeolite, 1-4 parts of potassium mineral powder, 0.5-1 part of ground phosphate rock, 0.5-1 part of calcium chloride and 0.1-0.5 part of synergist.
Furthermore, the straws are corn straws and/or wheat straws.
Further, when the wormcast is used, the wormcast is sieved by a 25-30-mesh sieve and then stacked for 6-9 days, and the moisture content of the wormcast is controlled to be 30-40% for later use.
The present invention selects wormcast as main organic raw material, and various organic wastes in the nature are fermented and then are quickly decomposed under the action of protease, lipase, cellulase and amylase in the earthworm digestive system, and are converted into nutrients which can be easily utilized by self or other organisms, and the wormcast is obtained after excretion. The earthworm cast is an ideal natural biological fertilizer, contains a large number of elements required by plants, a plurality of mineral elements and beneficial microorganisms, is beneficial to the growth of the plants, has uniform texture, black (gray) color fine crushed substances with natural soil smell, granular structures and capillary pores, has good ventilation, drainage and water retention properties and large specific surface area, provides a good living environment for a plurality of beneficial microorganisms, and has good capacity of absorbing and maintaining nutrient substances. Meanwhile, after the organic fertilizer is applied to soil, effective nutrients in the soil are released by mineralizing the surrounding soil, the content of humus in the soil can be increased, and the organic fertilizer can play a role in improving the soil better than common organic fertilizers.
Further, the microbial agent is prepared by mixing aspergillus polyvidus, pseudomonas paraflavus and bacillus subtilis according to a mass ratio of 1.
Furthermore, the preservation number of the aspergillus versicolor is CGMCC3.11555, the aspergillus versicolor is selected from China general microbiological preservation management center, the preservation address is the microbiological research institute of China academy of sciences No. 3 of Xilu No. 1 of Beijing north morning area, chaoyang, and the preservation date is 2008, 7 and 14 days;
the preservation number of the pseudomonas paratyphi is CGMCC1.15634, the pseudomonas paratyphi is selected from China general microbiological preservation management center, the preservation address is the microbiological research institute of China academy of sciences No. 3 of West Lu No. 1 of Beijing Korean district, and the preservation date is 2015, 2 months and 25 days;
the bacillus subtilis has a preservation number of CGMCC1.15832, is selected from China general microbiological preservation management center, has a preservation address of microorganism research institute of China academy of sciences No. 3, west Lu No. 1, north Cheng, chaoyang, beijing, and has a preservation date of 2016, 9 and 20 days.
The preparation method of the microbial agent comprises the following steps: respectively inoculating aspergillus polyvidus, pseudomonas paraflavus and bacillus subtilis into a 500mL shake flask of sterilized beef extract peptone culture medium, carrying out shake culture at 25-30 ℃ for 24h at 200r/min to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
furthermore, the beef extract peptone medium consists of: 5.0g of beef extract, 15.0g of peptone, 5.0g of sodium chloride, 1000mL of water, p H7.1-7.8, and sterilizing for 30min by high-pressure steam at 121-130 ℃.
Further, the porous zeolite is modified zeolite particles, and the specific preparation method comprises the following steps: weighing ferroferric oxide powder according to a feed-liquid ratio of 1g: uniformly dispersing 15mL of the mixture in acrylic emulsion, adding acetic acid accounting for 2% of the mass of the mixed solution and chitosan accounting for 0.5% of the mass of the mixed solution, and performing ultrasonic dispersion for 30-50min to obtain a dispersion liquid; then, mixing zeolite according to a solid-liquid ratio of 1g: dispersing 10mL of the sodium hydroxide solution in 2mol/l, soaking for 1-2h, and then mixing the sodium hydroxide solution and the water according to the volume ratio of 1:1, dispersing the dispersion liquid into sodium hydroxide solution of zeolite drop by drop, magnetically stirring for 10-20min, then filtering, washing with deionized water, and drying to obtain the modified zeolite particles.
Further, the synergist is sorbitol.
A preparation method of an organic fertilizer of a bio-control microbial inoculum comprises the following preparation steps:
(1) Preparing a microbial agent: respectively inoculating aspergillus polyvidus, pseudomonas paraflavus and bacillus subtilis into a 500mL shake flask of sterilized beef extract peptone culture medium, carrying out shake culture at 25-30 ℃ for 24h at 200r/min to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
(2) Preparing modified zeolite: weighing ferroferric oxide powder according to a feed-liquid ratio of 1g: uniformly dispersing 15mL of the mixture in acrylic emulsion, adding acetic acid accounting for 2% of the mass of the mixed solution and chitosan accounting for 0.5% of the mass of the mixed solution, and performing ultrasonic dispersion for 30-50min to obtain a dispersion liquid; then, mixing zeolite according to a solid-liquid ratio of 1g: dispersing 10mL of the sodium hydroxide solution in 2mol/l, soaking for 1-2h, and then mixing the sodium hydroxide solution and the water according to the volume ratio of 1:1, dispersing the dispersion liquid into a sodium hydroxide solution of zeolite drop by drop, magnetically stirring for 10-20min, then filtering, washing with deionized water, and drying to obtain modified zeolite particles;
(3) Weighing the raw materials according to the weight part, adjusting the humidity to 40-50%, mixing, performing pile fermentation, controlling the pile temperature to 45-65 ℃, the pile turning frequency to be 1 time/day, and the fermentation time to be 10-12 days, and drying and crushing after the fermentation is finished to obtain the organic fertilizer containing the biocontrol microbial inoculum.
Each of the raw materials of the present invention is commercially available.
According to the invention, aspergillus sydowii, pseudomonas paraflavus and Bacillus subtilis are screened as active microorganisms, and the Aspergillus sydowii can effectively remove pollutants such as heavy metal elements in soil and play a role in purifying soil; the pseudomonas paratyphi has better tolerance to various heavy metals, has plant growth promoting function, nitrogen fixation, phosphorus dissolution and siderophore secretion capacity, and can absorb and utilize insoluble or organic nutrients which cannot be directly or rapidly absorbed and utilized by plants in soil; the bacillus subtilis can secrete physiological active substances such as auxin (indoleacetic acid, IAA) and the like to promote plant growth, and meanwhile, when functional bacteria are propagated in the root system of a plant to form a dominant population, the growth and propagation of other pathogenic bacteria can be inhibited, so that the probability of infecting the root system of the plant is reduced. After the three components are mixed in equal proportion, the synergistic effect is achieved, and the dual effects of conditioning soil and promoting the growth and resisting diseases of plants are achieved together.
Meanwhile, the colonization ability has a determining effect on the application potential of the functional microorganisms, and if the functional bacteria cannot be colonized, the feasibility of the application of the functional bacteria is reduced. Therefore, the invention uses the magnetic nano ferroferric oxide powder and the acrylic emulsion to mix and improve the name, on one hand, the zeolite filled with the magnetic nano ferroferric oxide powder can effectively colonize and protect the microorganism, and the acrylic acid mixing and improving the name can realize the slow release of nutrient substances and microorganism active secretion, prolong the fertilizer efficiency and achieve two purposes.
Advantageous effects
According to the invention, wormcast, straw, bean pulp, humic acid and the like are used as raw materials, and aspergillus polyvidus, pseudomonas paraflavus and bacillus subtilis are screened as active microorganisms to prepare the bio-organic fertilizer, so that necessary nutrients can be provided for plant growth, the content of effective nutrients and organic matters can be increased, the soil structure can be improved, the soil microbial activity can be increased, the soil fertility can be further improved, the crop growth can be promoted, the use of inorganic chemical fertilizers can be replaced, the toxic effect of the inorganic chemical fertilizers on soil can be reduced, and the economic benefit and the social benefit are remarkable.
Detailed Description
The technical solution of the present invention is further described below with reference to specific embodiments, but is not limited thereto.
Example 1
An organic fertilizer containing a bio-control microbial inoculum is prepared from the following raw materials in parts by weight: 50 parts of straw, 50 parts of wormcast, 20 parts of bean pulp, 10 parts of corn flour, 10 parts of humic acid, 5 parts of microbial agent, 9 parts of porous zeolite, 1 part of potassium ore powder, 0.5 part of phosphate rock powder, 0.5 part of calcium chloride and 0.1 part of synergist.
The straw is corn straw.
When the wormcast is used, the wormcast is sieved by a sieve with 25-30 meshes and then stacked for 6 days, and the moisture content in the wormcast is controlled to be 30-40% for later use.
The microbial agent is prepared by mixing aspergillus polyvidus, pseudomonas paratuberculosis and bacillus subtilis according to the mass ratio of 1.
The preservation number of the aspergillus versicolor is CGMCC3.11555, the aspergillus versicolor is selected from China general microbiological preservation management center, the preservation address is the microbiological research institute of China academy of sciences No. 3, west Lu No. 1 Hopkins, chaozhong, chaoyang, and the preservation date is 2008, 7 and 14 days; the preservation number of the pseudomonas paratyphi is CGMCC1.15634, the pseudomonas paratyphi is selected from China general microbiological preservation management center, the preservation address is the microbiological research institute of China academy of sciences No. 3 of West Lu No. 1 of Beijing Korean district, and the preservation date is 2015, 2 months and 25 days; the bacillus subtilis has a preservation number of CGMCC1.15832, is selected from China general microbiological preservation management center, has a preservation address of microorganism research institute of China academy of sciences No. 3, west Lu No. 1, north Cheng, chaoyang, beijing, and has a preservation date of 2016, 9 and 20 days.
The preparation method of the microbial agent comprises the following steps: respectively inoculating aspergillus polyvidus, pseudomonas paraflavus and bacillus subtilis into a 500mL shake flask of sterilized beef extract peptone culture medium, carrying out shake culture at 25-30 ℃ for 24h at 200r/min to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
beef extract peptone medium: 5.0g of beef extract, 15.0g of peptone, 5.0g of sodium chloride, 1000mL of water, p H7.1-7.8, and sterilizing for 30min by high-pressure steam at 121-130 ℃.
The porous zeolite is modified zeolite particles, and the specific preparation method comprises the following steps: weighing ferroferric oxide powder according to a feed-liquid ratio of 1g: uniformly dispersing 15mL of the mixture in acrylic emulsion, adding acetic acid accounting for 2% of the mass of the mixed solution and chitosan accounting for 0.5% of the mass of the mixed solution, and performing ultrasonic dispersion for 30min to obtain a dispersion liquid; then, mixing zeolite according to a solid-liquid ratio of 1g: dispersing 10mL of the sodium hydroxide solution in 2mol/l, soaking for 1h, and then mixing the sodium hydroxide solution and the sodium hydroxide solution according to the volume ratio of 1:1, dispersing the dispersion liquid into sodium hydroxide solution of zeolite drop by drop, magnetically stirring for 10min, then filtering, washing with deionized water, and drying to obtain the modified zeolite particles.
The synergist is sorbitol.
A preparation method of an organic fertilizer of a bio-control microbial inoculum comprises the following preparation steps:
(1) Preparing a microbial agent: respectively inoculating aspergillus polyvidus, pseudomonas paraflavus and bacillus subtilis into a 500mL shake flask of sterilized beef extract peptone culture medium, carrying out shake culture at 25-30 ℃ for 24h at 200r/min to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
(2) Preparing modified zeolite: weighing ferroferric oxide powder according to a feed-liquid ratio of 1g: uniformly dispersing 15mL of the mixture into acrylic emulsion, adding acetic acid accounting for 2% of the mass of the mixed solution and chitosan accounting for 0.5% of the mass of the mixed solution, and performing ultrasonic dispersion for 30min to obtain a dispersion liquid; then, mixing zeolite according to a solid-liquid ratio of 1g: dispersing 10mL of the sodium hydroxide solution in 2mol/l, soaking for 1h, and then mixing the sodium hydroxide solution and the sodium hydroxide solution according to the volume ratio of 1:1, dispersing the dispersion liquid into a sodium hydroxide solution of zeolite drop by drop, magnetically stirring for 10min, then filtering, washing with deionized water, and drying to obtain modified zeolite particles;
(3) Weighing the raw materials according to the weight part, adjusting the humidity to 40-50%, mixing, performing pile fermentation, controlling the pile temperature to 45-65 ℃, the pile turning frequency to 1 time/day, and the fermentation time to 10 days, and drying and crushing after the fermentation is finished to obtain the organic fertilizer containing the biocontrol microbial inoculum.
Each of the starting materials in this example is commercially available.
Example 2
An organic fertilizer containing a bio-control microbial inoculum is prepared from the following raw materials in parts by weight: 70 parts of straw, 60 parts of wormcast, 35 parts of bean pulp, 20 parts of corn flour, 12 parts of humic acid, 10 parts of microbial agent, 13 parts of porous zeolite, 2 parts of potassium ore powder, 0.8 part of phosphate rock powder, 0.7 part of calcium chloride and 0.3 part of synergist.
The straw is wheat straw.
When the wormcast is used, the wormcast is sieved by a sieve with 25-30 meshes and then stacked for 7 days, and the moisture content in the wormcast is controlled to be 30-40% for later use.
The microbial agent is prepared by mixing aspergillus versicolor, pseudomonas paratuberans and bacillus subtilis according to a mass ratio of 1.
The preservation number of the aspergillus versicolor is CGMCC3.11555, the aspergillus versicolor is selected from China general microbiological preservation management center, the preservation address is the microbiological research institute of China academy of sciences No. 3, west Lu No. 1 Hopkins, chaozhong, chaoyang, and the preservation date is 2008, 7 and 14 days; the preservation number of the pseudomonas paratyphi is CGMCC1.15634, the pseudomonas paratyphi is selected from China general microbiological preservation management center, the preservation address is the microbiological research institute of China academy of sciences No. 3 of West Lu No. 1 of Beijing Korean district, and the preservation date is 2015, 2 months and 25 days; the bacillus subtilis has a preservation number of CGMCC1.15832, is selected from China general microbiological preservation management center, has a preservation address of microorganism research institute of China academy of sciences No. 3, west Lu No. 1, north Cheng, chaoyang, beijing, and has a preservation date of 2016, 9 and 20 days.
The preparation method of the microbial agent comprises the following steps: respectively inoculating aspergillus polyvidus, pseudomonas paraflavus and bacillus subtilis into a 500mL shake flask containing sterilized beef extract peptone culture medium, carrying out shake culture at 25-30 ℃ at 200r/min for 24h to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
beef extract peptone medium: 5.0g of beef extract, 15.0g of peptone, 5.0g of sodium chloride, 1000mL of water, p H7.1-7.8, and sterilizing for 30min by high-pressure steam at 121-130 ℃.
The porous zeolite is modified zeolite particles, and the specific preparation method comprises the following steps: weighing ferroferric oxide powder, and mixing the ferroferric oxide powder with the feed liquid ratio of 1g: uniformly dispersing 15mL of the mixture in acrylic emulsion, adding acetic acid accounting for 2% of the mass of the mixed solution and chitosan accounting for 0.5% of the mass of the mixed solution, and performing ultrasonic dispersion for 40min to obtain a dispersion liquid; then, mixing zeolite according to a solid-liquid ratio of 1g: dispersing 10mL of the sodium hydroxide solution in 2mol/l, soaking for 1h, and then mixing the sodium hydroxide solution and the sodium hydroxide solution according to the volume ratio of 1:1, dropwise dispersing the dispersion liquid into a sodium hydroxide solution of zeolite, magnetically stirring for 10min, then filtering, washing with deionized water, and drying to obtain the modified zeolite particles.
The synergist is sorbitol.
A preparation method of an organic fertilizer of a biocontrol microbial inoculum comprises the following preparation steps:
(1) Preparing a microbial agent: respectively inoculating aspergillus polyvidus, pseudomonas paraflavus and bacillus subtilis into a 500mL shake flask of sterilized beef extract peptone culture medium, carrying out shake culture at 25-30 ℃ for 24h at 200r/min to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
(2) Preparing modified zeolite: weighing ferroferric oxide powder according to a feed-liquid ratio of 1g: uniformly dispersing 15mL of the mixture in acrylic emulsion, adding acetic acid accounting for 2% of the mass of the mixed solution and chitosan accounting for 0.5% of the mass of the mixed solution, and performing ultrasonic dispersion for 40min to obtain a dispersion liquid; then, mixing zeolite according to a solid-liquid ratio of 1g: dispersing 10mL of the sodium hydroxide solution in 2mol/l, soaking for 1h, and then mixing the sodium hydroxide solution and the sodium hydroxide solution according to the volume ratio of 1:1, dropwise dispersing the dispersion liquid into a sodium hydroxide solution of zeolite, magnetically stirring for 10min, then filtering, washing with deionized water, and drying to obtain modified zeolite particles;
(3) Weighing the raw materials according to the weight part, adjusting the humidity to 40-50%, mixing, performing pile fermentation, controlling the pile temperature to 45-65 ℃, the pile turning frequency to be 1 time/day, and the fermentation time to be 11 days, and drying and crushing after the fermentation is finished to obtain the organic fertilizer containing the biocontrol microbial inoculum.
Each of the starting materials in this example is commercially available.
Example 3
An organic fertilizer containing a bio-control microbial inoculum is prepared from the following raw materials in parts by weight: 90 parts of straw, 80 parts of wormcast, 50 parts of bean pulp, 40 parts of corn flour, 15 parts of humic acid, 15 parts of microbial agent, 19 parts of porous zeolite, 4 parts of potassium ore powder, 1 part of phosphate rock powder, 1 part of calcium chloride and 0.5 part of synergist.
The straw is formed by mixing corn straw and wheat straw according to a mass ratio of 1.
When the wormcast is used, the wormcast is sieved by a sieve with 25-30 meshes and then stacked for 9 days, and the moisture content in the wormcast is controlled to be 30-40% for later use.
The microbial agent is prepared by mixing aspergillus versicolor, pseudomonas paratuberans and bacillus subtilis according to a mass ratio of 1.
The preservation number of the aspergillus versicolor is CGMCC3.11555, the aspergillus versicolor is selected from China general microbiological preservation management center, the preservation address is the microbiological research institute of China academy of sciences No. 3, west Lu No. 1 Hopkins, chaozhong, chaoyang, and the preservation date is 2008, 7 and 14 days;
the preservation number of the pseudomonas paratyphi is CGMCC1.15634, the pseudomonas paratyphi is selected from China general microbiological preservation management center, the preservation address is the microbiological research institute of China academy of sciences No. 3 of West Lu No. 1 of Beijing Korean district, and the preservation date is 2015, 2 months and 25 days;
the bacillus subtilis has a preservation number of CGMCC1.15832, is selected from China general microbiological preservation management center, has a preservation address of microorganism research institute of China academy of sciences No. 3 of Beijing, chaoyang, north Chen Xilu No. 1, and has a preservation date of 2016, 9 and 20 days.
The preparation method of the microbial agent comprises the following steps: respectively inoculating aspergillus polyvidus, pseudomonas paraflavus and bacillus subtilis into a 500mL shake flask of sterilized beef extract peptone culture medium, carrying out shake culture at 25-30 ℃ for 24h at 200r/min to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
the beef extract peptone culture medium comprises the following components: 5.0g of beef extract, 15.0g of peptone, 5.0g of sodium chloride, 1000mL of water, p H7.1-7.8, and sterilizing for 30min by high-pressure steam at 121-130 ℃.
The porous zeolite is modified zeolite particles, and the specific preparation method comprises the following steps: weighing ferroferric oxide powder according to a feed-liquid ratio of 1g: uniformly dispersing 15mL of the mixture in acrylic emulsion, adding acetic acid accounting for 2% of the mass of the mixed solution and chitosan accounting for 0.5% of the mass of the mixed solution, and performing ultrasonic dispersion for 50min to obtain a dispersion liquid; then, mixing zeolite according to a solid-liquid ratio of 1g: dispersing 10mL of the sodium hydroxide solution in 2mol/l, soaking for 2 hours, and then mixing the sodium hydroxide solution and the sodium hydroxide solution according to the volume ratio of 1:1, dispersing the dispersion liquid into a sodium hydroxide solution of zeolite drop by drop, magnetically stirring for 20min, then filtering, washing with deionized water, and drying to obtain the modified zeolite particles.
The synergist is sorbitol.
A preparation method of an organic fertilizer of a bio-control microbial inoculum comprises the following preparation steps:
(1) Preparing a microbial agent: respectively inoculating aspergillus polyvidus, pseudomonas paraflavus and bacillus subtilis into a 500mL shake flask of sterilized beef extract peptone culture medium, carrying out shake culture at 25-30 ℃ for 24h at 200r/min to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
(2) Preparing modified zeolite: weighing ferroferric oxide powder according to a feed-liquid ratio of 1g: uniformly dispersing 15mL of the mixture in acrylic emulsion, adding acetic acid accounting for 2% of the mass of the mixed solution and chitosan accounting for 0.5% of the mass of the mixed solution, and performing ultrasonic dispersion for 50min to obtain a dispersion liquid; then, mixing zeolite according to a solid-liquid ratio of 1g: dispersing 10mL of the sodium hydroxide solution in 2mol/l, soaking for 2 hours, and then mixing the sodium hydroxide solution and the sodium hydroxide solution according to the volume ratio of 1:1, dropwise dispersing the dispersion liquid into a sodium hydroxide solution of zeolite, magnetically stirring for 20min, then filtering, washing with deionized water, and drying to obtain modified zeolite particles;
(3) Weighing the raw materials according to the weight part, adjusting the humidity to 40-50%, mixing, performing pile fermentation, controlling the pile temperature to 45-65 ℃, the pile turning frequency to be 1 time/day, and the fermentation time to be 12 days, and drying and crushing after the fermentation is finished to obtain the organic fertilizer containing the biocontrol microbial inoculum.
Each of the starting materials in this example is commercially available.
Comparative example 1
An organic fertilizer containing a bio-control microbial inoculum is prepared from the following raw materials in parts by weight: 90 parts of straw, 80 parts of wormcast, 50 parts of bean pulp, 40 parts of corn flour, 15 parts of humic acid, 15 parts of microbial agent, 19 parts of porous zeolite, 4 parts of potassium ore powder, 1 part of phosphate rock powder, 1 part of calcium chloride and 0.5 part of synergist.
The straw is formed by mixing corn straw and wheat straw according to a mass ratio of 1.
When the wormcast is used, the wormcast is sieved by a sieve with 25-30 meshes and then stacked for 9 days, and the moisture content in the wormcast is controlled to be 30-40% for later use.
The microbial agent is prepared by mixing aspergillus versicolor, pseudomonas paratuberans and bacillus subtilis according to a mass ratio of 1.
The preservation number of the Aspergillus sydowii is CGMCC3.11555, the Aspergillus sydowii is selected from China general microbiological preservation management center, the preservation address is the microbial research institute of China academy of sciences No. 3 of Xilu No. 1 of Beijing, chaoyang, beijing, and the preservation date is 2008, 7 months and 14 days;
the preservation number of the pseudomonas paratyphi is CGMCC1.15634, the pseudomonas paratyphi is selected from China general microbiological preservation management center, the preservation address is the microbiological research institute of China academy of sciences No. 3 of West Lu No. 1 of Beijing Korean district, and the preservation date is 2015, 2 months and 25 days;
the bacillus subtilis has a preservation number of CGMCC1.15832, is selected from China general microbiological preservation management center, has a preservation address of microorganism research institute of China academy of sciences No. 3, west Lu No. 1, north Cheng, chaoyang, beijing, and has a preservation date of 2016, 9 and 20 days.
The preparation method of the microbial agent comprises the following steps: respectively inoculating aspergillus polyvidus, pseudomonas paraflavus and bacillus subtilis into a 500mL shake flask of sterilized beef extract peptone culture medium, carrying out shake culture at 25-30 ℃ for 24h at 200r/min to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
the beef extract peptone medium comprises the following components: 5.0g of beef extract, 15.0g of peptone, 5.0g of sodium chloride, 1000mL of water, p H7.1-7.8, and sterilizing for 30min by high-pressure steam at 121-130 ℃.
The porous zeolite is a common zeolite particle.
The synergist is sorbitol.
A preparation method of an organic fertilizer of a bio-control microbial inoculum comprises the following preparation steps:
(1) Preparing a microbial agent: respectively inoculating aspergillus polyvidus, pseudomonas paraflavus and bacillus subtilis into a 500mL shake flask of sterilized beef extract peptone culture medium, carrying out shake culture at 25-30 ℃ for 24h at 200r/min to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
(2) Weighing the raw materials according to the weight part, adjusting the humidity to 40-50%, mixing, performing pile fermentation, controlling the pile temperature to 45-65 ℃, the pile turning frequency to be 1 time/day, and the fermentation time to be 12 days, and drying and crushing after the fermentation is finished to obtain the organic fertilizer containing the biocontrol microbial inoculum.
Each of the materials of this comparative example was commercially available.
This comparative example uses commercial zeolite particles as they are, except that the zeolite has not been modified, and the remaining raw materials and preparation process are the same as in example 3.
Comparative example 2
An organic fertilizer containing a bio-control microbial inoculum is prepared from the following raw materials in parts by weight: 90 parts of straw, 80 parts of wormcast, 50 parts of bean pulp, 40 parts of corn flour, 15 parts of humic acid, 15 parts of microbial agent, 19 parts of porous zeolite, 4 parts of potassium ore powder, 1 part of phosphate rock powder, 1 part of calcium chloride and 0.5 part of synergist.
The straw is formed by mixing corn straw and wheat straw according to a mass ratio of 1.
When the wormcast is used, the wormcast is sieved by a sieve with 25-30 meshes and then stacked for 9 days, and the moisture content in the wormcast is controlled to be 30-40% for later use.
The microbial agent is prepared by mixing pseudomonas parapoxeroides and bacillus subtilis according to the mass ratio of 1.
The preservation number of the pseudomonas paratyphi is CGMCC1.15634, the pseudomonas paratyphi is selected from China general microbiological preservation management center, the preservation address is the microbiological research institute of China academy of sciences No. 3 of West Lu No. 1 of Beijing Korean district, and the preservation date is 2015, 2 months and 25 days;
the bacillus subtilis has a preservation number of CGMCC1.15832, is selected from China general microbiological preservation management center, has a preservation address of microorganism research institute of China academy of sciences No. 3, west Lu No. 1, north Cheng, chaoyang, beijing, and has a preservation date of 2016, 9 and 20 days.
The preparation method of the microbial agent comprises the following steps: respectively inoculating pseudomonas paratungensis and bacillus subtilis into a 500mL shake flask of sterilized beef extract peptone culture medium, carrying out shake culture at 25-30 ℃ for 24h at 200r/min to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
the beef extract peptone medium comprises the following components: 5.0g of beef extract, 15.0g of peptone, 5.0g of sodium chloride, 1000mL of water, p H7.1-7.8, and sterilizing for 30min by high-pressure steam at 121-130 ℃.
The porous zeolite is modified zeolite particles, and the specific preparation method comprises the following steps: weighing ferroferric oxide powder according to a feed-liquid ratio of 1g: uniformly dispersing 15mL of the mixture into acrylic emulsion, adding acetic acid accounting for 2% of the mass of the mixed solution and chitosan accounting for 0.5% of the mass of the mixed solution, and performing ultrasonic dispersion for 50min to obtain a dispersion liquid; then, mixing zeolite according to a solid-liquid ratio of 1g: dispersing 10mL of the sodium hydroxide solution in 2mol/l, soaking for 2 hours, and then mixing the sodium hydroxide solution and the sodium hydroxide solution according to the volume ratio of 1:1, dropwise dispersing the dispersion liquid into a sodium hydroxide solution of zeolite, magnetically stirring for 20min, then filtering, washing with deionized water, and drying to obtain the modified zeolite particles.
The synergist is sorbitol.
A preparation method of an organic fertilizer of a bio-control microbial inoculum comprises the following preparation steps:
(1) Preparing a microbial agent: respectively inoculating pseudomonas paratungensis and bacillus subtilis into 500mL shake flasks of sterilized beef extract peptone culture medium, carrying out shake culture at 25-30 ℃ at 200r/min for 24h to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
(2) Preparing modified zeolite: weighing ferroferric oxide powder according to a feed-liquid ratio of 1g: uniformly dispersing 15mL of the mixture in acrylic emulsion, adding acetic acid accounting for 2% of the mass of the mixed solution and chitosan accounting for 0.5% of the mass of the mixed solution, and performing ultrasonic dispersion for 50min to obtain a dispersion liquid; then, mixing zeolite according to a solid-liquid ratio of 1g: dispersing 10mL of the sodium hydroxide solution in 2mol/l, soaking for 2 hours, and then mixing the sodium hydroxide solution and the sodium hydroxide solution according to the volume ratio of 1:1, dropwise dispersing the dispersion liquid into a sodium hydroxide solution of zeolite, magnetically stirring for 20min, then filtering, washing with deionized water, and drying to obtain modified zeolite particles;
(3) Weighing the raw materials according to the weight part, adjusting the humidity to 40-50%, mixing, performing pile fermentation, controlling the pile temperature to 45-65 ℃, the pile turning frequency to be 1 time/day, and the fermentation time to be 12 days, and drying and crushing after the fermentation is finished to obtain the organic fertilizer containing the biocontrol microbial inoculum.
Each of the materials of this comparative example was commercially available.
The comparative example is the same as example 3 except that no Aspergillus sydowii is added to the microbial agent.
Comparative example 3
An organic fertilizer containing a bio-control microbial inoculum is prepared from the following raw materials in parts by weight: 90 parts of straw, 80 parts of wormcast, 50 parts of bean pulp, 40 parts of corn flour, 15 parts of humic acid, 15 parts of microbial agent, 19 parts of porous zeolite, 4 parts of potassium ore powder, 1 part of phosphate rock powder, 1 part of calcium chloride and 0.5 part of synergist.
The straw is formed by mixing corn straw and wheat straw according to a mass ratio of 1.
When the wormcast is used, the wormcast is sieved by a sieve with 25-30 meshes and then stacked for 9 days, and the moisture content in the wormcast is controlled to be 30-40% for later use.
The microbial agent is prepared by mixing aspergillus versipellis and bacillus subtilis according to the mass ratio of 1.
The preservation number of the aspergillus versicolor is CGMCC3.11555, the aspergillus versicolor is selected from China general microbiological preservation management center, the preservation address is the microbiological research institute of China academy of sciences No. 3, west Lu No. 1 Hopkins, chaozhong, chaoyang, and the preservation date is 2008, 7 and 14 days;
the bacillus subtilis has a preservation number of CGMCC1.15832, is selected from China general microbiological preservation management center, has a preservation address of microorganism research institute of China academy of sciences No. 3, west Lu No. 1, north Cheng, chaoyang, beijing, and has a preservation date of 2016, 9 and 20 days.
The preparation method of the microbial agent comprises the following steps: respectively inoculating aspergillus polyvidus and bacillus subtilis into a 500mL shake flask of sterilized beef extract peptone culture medium, carrying out shake culture at 25-30 ℃ for 24h at 200r/min to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
the beef extract peptone medium comprises the following components: 5.0g of beef extract, 15.0g of peptone, 5.0g of sodium chloride, 1000mL of water, p H7.1-7.8, and sterilizing for 30min by high-pressure steam at 121-130 ℃.
The porous zeolite is modified zeolite particles, and the specific preparation method comprises the following steps: weighing ferroferric oxide powder according to a feed-liquid ratio of 1g: uniformly dispersing 15mL of the mixture in acrylic emulsion, adding acetic acid accounting for 2% of the mass of the mixed solution and chitosan accounting for 0.5% of the mass of the mixed solution, and performing ultrasonic dispersion for 50min to obtain a dispersion liquid; then, mixing zeolite according to a solid-liquid ratio of 1g: dispersing 10mL of the sodium hydroxide solution in 2mol/l, soaking for 2 hours, and then mixing the sodium hydroxide solution and the sodium hydroxide solution according to the volume ratio of 1:1, dropwise dispersing the dispersion liquid into a sodium hydroxide solution of zeolite, magnetically stirring for 20min, then filtering, washing with deionized water, and drying to obtain the modified zeolite particles.
The synergist is sorbitol.
A preparation method of an organic fertilizer of a bio-control microbial inoculum comprises the following preparation steps:
(1) Preparing a microbial agent: respectively inoculating aspergillus polyvidus and bacillus subtilis into a 500mL shake flask of sterilized beef extract peptone culture medium, carrying out shake culture at 25-30 ℃ for 24h at 200r/min to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
(2) Preparation of modified zeolite: weighing ferroferric oxide powder according to a feed-liquid ratio of 1g: uniformly dispersing 15mL of the mixture in acrylic emulsion, adding acetic acid accounting for 2% of the mass of the mixed solution and chitosan accounting for 0.5% of the mass of the mixed solution, and performing ultrasonic dispersion for 50min to obtain a dispersion liquid; then, mixing zeolite according to a solid-liquid ratio of 1g: dispersing 10mL of the sodium hydroxide solution in 2mol/l, soaking for 2 hours, and then mixing the sodium hydroxide solution and the sodium hydroxide solution according to the volume ratio of 1:1, dropwise dispersing the dispersion liquid into a sodium hydroxide solution of zeolite, magnetically stirring for 20min, then filtering, washing with deionized water, and drying to obtain modified zeolite particles;
(3) Weighing the raw materials according to the weight parts, adjusting the humidity to 40-50%, mixing, performing pile fermentation, controlling the pile temperature to 45-65 ℃, turning the pile frequency for 1 time/day, fermenting for 12 days, drying and crushing after the fermentation is finished to obtain the organic fertilizer containing the biocontrol microbial inoculum.
Each of the materials of this comparative example was commercially available.
The comparative example is the same as example 3 except that Pseudomonas parachutista is not added in the microbial agent.
Comparative example 4
An organic fertilizer containing a bio-control microbial inoculum is prepared from the following raw materials in parts by weight: 90 parts of straw, 80 parts of wormcast, 50 parts of bean pulp, 40 parts of corn flour, 15 parts of humic acid, 15 parts of microbial agent, 19 parts of porous zeolite, 4 parts of potassium ore powder, 1 part of phosphate rock powder, 1 part of calcium chloride and 0.5 part of synergist.
The straw is formed by mixing corn straw and wheat straw according to a mass ratio of 1.
When the wormcast is used, the wormcast is sieved by a sieve with 25-30 meshes and then stacked for 9 days, and the moisture content in the wormcast is controlled to be 30-40% for later use.
The microbial agent is prepared by mixing aspergillus versipellis and pseudomonas paratuberans according to the mass ratio of 1.
The preservation number of the aspergillus versicolor is CGMCC3.11555, the aspergillus versicolor is selected from China general microbiological preservation management center, the preservation address is the microbiological research institute of China academy of sciences No. 3, west Lu No. 1 Hopkins, chaozhong, chaoyang, and the preservation date is 2008, 7 and 14 days;
the preservation number of the pseudomonas paratyphi is CGMCC1.15634, the pseudomonas paratyphi is selected from China general microbiological preservation management center, the preservation address is the microbiological research institute of China academy of sciences No. 3 of West Lu No. 1 of Beijing Korean district, and the preservation date is 2015, 2 months and 25 days;
the preparation method of the microbial agent comprises the following steps: respectively inoculating aspergillus polyvidus and pseudomonas paraflava into a sterilized beef extract peptone culture medium 500mL shake flask, carrying out shake culture at 25-30 ℃ at 200r/min for 24h to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
the beef extract peptone medium comprises the following components: 5.0g of beef extract, 15.0g of peptone, 5.0g of sodium chloride, 1000mL of water, p H7.1-7.8, and sterilizing for 30min by high-pressure steam at 121-130 ℃.
The porous zeolite is modified zeolite particles, and the specific preparation method comprises the following steps: weighing ferroferric oxide powder according to a feed-liquid ratio of 1g: uniformly dispersing 15mL of the mixture in acrylic emulsion, adding acetic acid accounting for 2% of the mass of the mixed solution and chitosan accounting for 0.5% of the mass of the mixed solution, and performing ultrasonic dispersion for 50min to obtain a dispersion liquid; then, mixing zeolite according to a solid-liquid ratio of 1g: dispersing 10mL of the solution in 2mol/l sodium hydroxide solution, soaking for 2h, and then mixing the solution according to the volume ratio of 1:1, dropwise dispersing the dispersion liquid into a sodium hydroxide solution of zeolite, magnetically stirring for 20min, then filtering, washing with deionized water, and drying to obtain the modified zeolite particles.
The synergist is sorbitol.
A preparation method of an organic fertilizer of a bio-control microbial inoculum comprises the following preparation steps:
(1) Preparing a microbial agent: respectively inoculating aspergillus polyvidus and pseudomonas paraflava into a sterilized beef extract peptone culture medium 500mL shake flask, carrying out shake culture at 25-30 ℃ at 200r/min for 24h to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
(2) Preparing modified zeolite: weighing ferroferric oxide powder according to a feed-liquid ratio of 1g: uniformly dispersing 15mL of the mixture in acrylic emulsion, adding acetic acid accounting for 2% of the mass of the mixed solution and chitosan accounting for 0.5% of the mass of the mixed solution, and performing ultrasonic dispersion for 50min to obtain a dispersion liquid; then, mixing zeolite according to a solid-liquid ratio of 1g: dispersing 10mL of the sodium hydroxide solution in 2mol/l, soaking for 2 hours, and then mixing the sodium hydroxide solution and the sodium hydroxide solution according to the volume ratio of 1:1, dropwise dispersing the dispersion liquid into a sodium hydroxide solution of zeolite, magnetically stirring for 20min, then filtering, washing with deionized water, and drying to obtain modified zeolite particles;
(3) Weighing the raw materials according to the weight part, adjusting the humidity to 40-50%, mixing, performing pile fermentation, controlling the pile temperature to 45-65 ℃, the pile turning frequency to be 1 time/day, and the fermentation time to be 12 days, and drying and crushing after the fermentation is finished to obtain the organic fertilizer containing the biocontrol microbial inoculum.
Each of the materials of this comparative example was commercially available.
In the comparative example, the raw materials and the preparation process are the same as those in example 3 except that no Bacillus subtilis is added to the microbial agent.
Performance testing
The fertilizers obtained in examples 1 to 3 of the present invention and comparative examples 1 to 4 were subjected to performance tests
Measurement method
Effective viable count: the determination is carried out according to the method in NY/T2321-2013 microbial fertilizer product inspection regulations.
The technical indexes of the bio-organic fertilizer product are as follows: the determination was carried out according to the method in NY 884-2012 "Bio-organic fertilizers".
The results are shown in table 1:
TABLE 1 product Performance index
Planting test
Test work: corn "denghai 605"
Fertilizer to be tested: the fertilizers obtained in examples 1-3 and comparative examples 1-4 were applied at a rate of 3000kg/hm 2 The fertilizer is used as a base fertilizer to be applied to a soil plough layer at one time, and no additional fertilizer is applied in the whole corn growth period. Other management measures are kept consistent with local. A blank control was set, i.e. no fertilizer was applied.
And (3) collecting continuous 30-strain ears with consistent growth vigor in each cell in the corn harvesting period, and determining the above-ground biomass, the corn yield and yield forming factors.
Surface soil (0-20 cm) samples are collected after the corns are harvested, and soil samples are collected in each cell according to an S-shaped sampling method. When soil is taken, the soil auger is drilled to the depth of 20cm at one time, 3 times of repetition are collected in each district, 3 repeated samples in the same district are mixed into one sample, visible impurities such as gravel and plant root systems are removed, and the sample is brought back to a laboratory to measure the content of soil nutrients and the activity of enzyme.
Soil microbiological assay
The number of three microorganism groups of soil bacteria, fungi and actinomycetes is measured by a dilution plate segregation counting method, and is expressed as the number of colonies contained in each gram of dry soil (cfu). Culturing bacteria in beef extract peptone culture medium at 37 deg.C for 2-3d, culturing fungi in Martin-Bengal culture medium at 27-28 deg.C for 3d, culturing actinomycetes in Gao's I culture medium at 27-28 deg.C for 6d, recording colony count of each strain, and calculating colony count (cfu) per gram of dry soil according to soil water content.
Soil enzyme activity assay
The soil urease activity is measured by adopting an indigo colorimetric method, the soil sucrase activity is measured by adopting a 3, 5-dinitrosalicylic acid colorimetric method, the soil alkaline phosphatase activity is measured by adopting a disodium phenylphosphate colorimetric method, and the soil catalase activity is measured by adopting a potassium permanganate oxidation method.
TABLE 2 planting test and soil index
As can be seen from the data in the table, the fertilizer test group obtained in the embodiment of the invention has the advantages that the corn yield is far higher than that of the comparative example and the blank test group, and the regulation on the physical and chemical properties and the biological activity of soil is also very obvious. The comparative examples 2 to 4 in which the composition of the microbial inoculum was changed and the comparative example 1 in which zeolite was not modified exhibited an obvious tendency to decline in fertilizer efficiency, both in fertilizer efficiency and in soil conditioning effect, because the synergistic effect between microorganisms and the protective effect of modified zeolite disappeared and the microorganisms were unable to efficiently exert biological activity, the fertilizer efficiency declined. Therefore, the composition and the preparation process of the organic fertilizer are the key points for realizing the stable fertilizer efficiency and the soil regulation effect of the invention, and the effect is weak in the absence of the organic fertilizer.
It should be noted that the above-mentioned embodiments are only some of the preferred modes for implementing the invention, and not all of them. Obviously, all other embodiments obtained by persons of ordinary skill in the art based on the above-mentioned embodiments of the present invention without any creative effort shall fall within the protection scope of the present invention.
Claims (5)
1. An organic fertilizer containing a bio-control microbial inoculum is characterized by being prepared from the following raw materials in parts by weight: 50-90 parts of straw, 50-80 parts of wormcast, 20-50 parts of bean pulp, 10-40 parts of corn flour, 10-15 parts of humic acid, 5-15 parts of microbial agent, 9-19 parts of porous zeolite, 1-4 parts of potassium mineral powder, 0.5-1 part of ground phosphate rock, 0.5-1 part of calcium chloride and 0.1-0.5 part of synergist;
the microbial agent is prepared by mixing aspergillus versicolor, pseudomonas paratuberans and bacillus subtilis according to a mass ratio of 1; the preservation number of the aspergillus versicolor is CGMCC3.11555, and the aspergillus versicolor is selected from China general microbiological preservation management center; the preservation number of the pseudomonas paratyphi is CGMCC1.15634, and the pseudomonas paratyphi is selected from China general microbiological preservation management center; the bacillus subtilis has a preservation number of CGMCC1.15832 and is selected from China general microbiological preservation management center;
the porous zeolite is modified zeolite particles, and the specific preparation method comprises the following steps: weighing ferroferric oxide powder according to a feed-liquid ratio of 1g: uniformly dispersing 15mL of the mixture into acrylic emulsion, adding acetic acid accounting for 2% of the mass of the mixed solution and chitosan accounting for 0.5% of the mass of the mixed solution, and performing ultrasonic dispersion for 30-50min to obtain a dispersion liquid; then, mixing zeolite according to a solid-liquid ratio of 1g: dispersing 10mL of the sodium hydroxide solution in 2mol/l, soaking for 1-2h, and then mixing the sodium hydroxide solution and the water according to the volume ratio of 1:1, dropwise dispersing the dispersion liquid into a sodium hydroxide solution of zeolite, magnetically stirring for 10-20min, then filtering, washing with deionized water, and drying to obtain modified zeolite particles.
2. The organic fertilizer containing the biocontrol microbial inoculum as in claim 1, wherein the straws are corn straws and/or wheat straws.
3. The organic fertilizer containing the biocontrol microbial inoculum as in claim 1, wherein when the wormcast is used, the wormcast is sieved by a sieve with 25-30 meshes and then stacked for 6-9 days, and the moisture content in the wormcast is controlled to be 30-40% for later use.
4. The organic fertilizer containing the biocontrol microbial inoculum as in claim 1, wherein the synergist is sorbitol.
5. A preparation method of the organic fertilizer of the biocontrol microbial inoculum as defined in any one of claims 1-4, which is characterized by comprising the following preparation steps:
(1) Preparing a microbial agent: respectively inoculating aspergillus polyvidus, pseudomonas paratuberans and bacillus subtilis into a sterilized beef extract peptone culture medium 500ml shake flask, carrying out shake culture at 25-30 ℃ at 200r/min for 24h to respectively obtain bacterial suspensions, and mixing the bacterial suspensions according to a volume ratio of 1;
(2) Preparing modified zeolite: weighing ferroferric oxide powder according to a feed-liquid ratio of 1g: uniformly dispersing 15mL of the mixture in acrylic emulsion, adding acetic acid accounting for 2% of the mass of the mixed solution and chitosan accounting for 0.5% of the mass of the mixed solution, and performing ultrasonic dispersion for 30-50min to obtain a dispersion liquid; then, mixing zeolite according to a solid-liquid ratio of 1g: dispersing 10mL of the sodium hydroxide solution in 2mol/l, soaking for 1-2h, and then mixing the sodium hydroxide solution and the water according to the volume ratio of 1:1, dropwise dispersing the dispersion liquid into a sodium hydroxide solution of zeolite, magnetically stirring for 10-20min, filtering, washing with deionized water, and drying to obtain modified zeolite particles;
(3) Weighing the raw materials according to the weight part, adjusting the humidity to 40-50%, mixing, performing pile fermentation, controlling the pile temperature to 45-65 ℃, the pile turning frequency to be 1 time/day, and the fermentation time to be 10-12 days, and drying and crushing after the fermentation is finished to obtain the organic fertilizer containing the biocontrol microbial inoculum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210791717.7A CN114907161B (en) | 2022-07-05 | 2022-07-05 | Organic fertilizer containing biocontrol microbial inoculum and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210791717.7A CN114907161B (en) | 2022-07-05 | 2022-07-05 | Organic fertilizer containing biocontrol microbial inoculum and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114907161A CN114907161A (en) | 2022-08-16 |
| CN114907161B true CN114907161B (en) | 2023-03-21 |
Family
ID=82772425
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210791717.7A Active CN114907161B (en) | 2022-07-05 | 2022-07-05 | Organic fertilizer containing biocontrol microbial inoculum and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114907161B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115707675A (en) * | 2022-10-17 | 2023-02-21 | 镇江丰泰农业科技有限公司 | Special selenium-rich and zinc-rich functional fertilizer for rice |
| CN115777727B (en) * | 2022-11-21 | 2023-12-12 | 浙江大学山东(临沂)现代农业研究院 | Microbial agent for resisting root rot and preparation method thereof |
| CN117466684B (en) * | 2023-11-01 | 2024-10-01 | 山东禾之源现代农业发展有限公司 | Sustained-release bio-organic fertilizer and preparation method thereof |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1304912A (en) * | 2000-12-31 | 2001-07-25 | 李承海 | Process for preparing microecological organic fertilizer |
| CN105037041A (en) * | 2015-08-06 | 2015-11-11 | 青岛点石文具用品有限公司 | Compound efficient disease-preventing bio-fertilizer |
| CN107827568A (en) * | 2017-11-24 | 2018-03-23 | 湖北亿隆生物科技有限公司 | A kind of organic substrate fertilizer for tobacco planting |
| CN107827569A (en) * | 2017-11-24 | 2018-03-23 | 湖北亿隆生物科技有限公司 | A kind of organic substrate fertilizer for flower planting |
| CN107739287A (en) * | 2017-12-18 | 2018-02-27 | 蒲江县樱红缘农业开发有限公司 | A kind of organic composite fertilizer |
| CN108585216A (en) * | 2018-04-16 | 2018-09-28 | 佛山市尚柏科技有限公司 | A kind of organic pollution complex microorganism degradation agent and preparation method |
| CN113412770A (en) * | 2021-05-26 | 2021-09-21 | 江苏沿江地区农业科学研究所 | Structural matrix of nursery stock in saline-alkali soil and preparation method thereof |
| CN113273475A (en) * | 2021-07-07 | 2021-08-20 | 江苏淮安国峰清源生物燃气有限责任公司 | Rice seedling raising substrate and preparation method thereof |
| CN114467988B (en) * | 2022-04-01 | 2023-06-16 | 江西湘金农林业有限公司 | Pesticide for preventing and controlling agricultural diseases and insect pests and preparation method thereof |
-
2022
- 2022-07-05 CN CN202210791717.7A patent/CN114907161B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN114907161A (en) | 2022-08-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN114907161B (en) | Organic fertilizer containing biocontrol microbial inoculum and preparation method thereof | |
| CN103232944B (en) | Microorganism bacterium agent for straw and excrement mixed composting | |
| CN106754461B (en) | Biological floc and preparation method and application thereof | |
| CN101497542B (en) | Biological preparation containing plant growth promoting bacteria, as well as preparation and use thereof | |
| CN101481666B (en) | Plant promoting bacteria, microbial preparation containing the same and preparation thereof | |
| CN104557335A (en) | Organic-inorganic compound bacterial fertilizer and preparation method of compound bacterial fertilizer | |
| CN110590421A (en) | Drought-resisting and water-retaining soil conditioner for hilly and mountainous areas and preparation method thereof | |
| CN106045714A (en) | Organic fertilizer produced from tea seed meal and tea seed shells and preparing method thereof | |
| CN116103204B (en) | Microbial decomposing inoculant for organic compost and preparation method thereof | |
| CN113481119B (en) | Composite microbial agent for improving phosphorus utilization rate | |
| CN110437000A (en) | A kind of microbial manure and its preparation method and application | |
| CN108034650A (en) | A kind of microbial bacteria agent carrier and preparation method and application | |
| CN113234616A (en) | Bacillus licheniformis, saline-alkali tolerant microbial agent and preparation method of biomass saline-alkali soil conditioner | |
| CN113321547A (en) | Rhizosphere growth promoting water-soluble microbial fertilizer and preparation method thereof | |
| CN107176891A (en) | It is a kind of to promote the biological agent and its production technology of stalk fast degradation | |
| CN106986696A (en) | Utilize soil conditioner obtained by biological fermentation bed waste material and preparation method thereof | |
| CN113773987B (en) | Biological agent for improving aerobic fermentation efficiency of organic waste and preparation method thereof | |
| CN113234446A (en) | Biomass saline-alkali soil conditioner | |
| CN101759501B (en) | Complex microorganisms flora grain type ascharite fertilizer and preparation method thereof | |
| CN112209778A (en) | Novel slag soil conditioner and preparation method thereof | |
| CN101759500A (en) | Mineral fertilizer containing complex microbial community and preparation method thereof | |
| CN107628894A (en) | Composite bacteria agent increase soil fertility and its preparation method and application | |
| CN115044509A (en) | Preparation process of growth-promoting microbial agent | |
| CN101468924A (en) | Biological organic fertilizer and production method thereof | |
| CN113372168A (en) | Biological humic acid fertilizer and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |