CN114894728A - Micro-fluidic chip and spectral information detection system - Google Patents

Abstract

The invention relates to the technical field of spectrum detection, and provides a micro-fluidic chip and a spectrum information detection system, wherein the micro-fluidic chip comprises: the chip comprises a transparent chip bottom plate, wherein a sample flow channel is formed on the chip bottom plate; a first groove is formed on the chip bottom plate and comprises a first cambered surface and a second cambered surface which are relatively sunken so as to construct an on-chip micro lens, the on-chip micro lens is positioned on the side surface of the sample flow channel, and the optical axis of the on-chip micro lens is vertical to the sample flow channel; and a second groove is formed on the chip bottom plate, the second groove is triangular so as to construct an on-chip prism, and the on-chip prism is positioned on one side of the on-chip micro lens, which is back to the sample flow channel. The micro-fluidic chip can obtain a spectrum consisting of monochromatic light with different wavelengths, is convenient for improving the subsequent detection precision, and has the characteristics of simple structure, small volume, low cost, portability, high stability and reliability, suitability for instant detection occasions and the like.

Description

Microfluidic chip and spectral information detection system

technical field

The invention relates to the technical field of spectral detection, in particular to a microfluidic chip and a spectral information detection system.

Background technique

Spectroscopic detection is widely used in research in various fields such as cell biology, protein engineering, drug discovery, medical diagnosis, and biotechnology. Current frontier biological research and clinical medical research, such as flow spectroscopic detection, biochemical fluorescence detection and chemiluminescence phenomenon research, can analyze the spectral information of different biological samples by detecting the dispersion of multiple fluorophores. This spectral-based signal detection method is very efficient for detecting the signal of a specific fluorophore.

So far, the clinical instruments that can collect and analyze spectral signals, the first is the use of dichroic mirrors and bandpass filters to select specific spectral bands for the use of point detectors such as photomultiplier tubes (PMTs). The disadvantage of this type of instrument is that the collected spectral signal has low resolution and is not suitable for detecting severely overlapping spectral signals; the second type is to emit scattered photons to the detector through additional off-chip optical elements such as prisms or gratings However, such devices are often combined with complex optical lens combinations, requiring a variety of additional off-chip optical components and optical systems to cooperate with each other, resulting in long-distance optical propagation between components. Excessive energy loss not only results in low detection accuracy, excessive equipment volume, and high manufacturing cost, but also requires professionals to operate, which is not suitable for point-of-care inspection (POCT) applications.

SUMMARY OF THE INVENTION

The invention provides a microfluidic chip and a spectral information detection system. By integrating a sample flow channel, an on-chip microlens and an on-chip prism on the microfluidic chip, spectra composed of monochromatic light of different wavelengths can be obtained, so as to facilitate the Subsequently, multiple fluorophores with highly overlapping emission spectra can be simultaneously detected to obtain higher resolution; and the loss of optical signals can be reduced, the detection accuracy can be improved, and it has the advantages of simple structure, small size, low cost, portability, stability and reliability. It has the characteristics of high performance and suitable for instant detection occasions.

The present invention provides a microfluidic chip, comprising:

a transparent chip base plate, on which a sample flow channel is formed;

A first groove is formed on the chip bottom plate, and the first groove includes two relatively concave first arc surfaces and second arc surfaces to construct an on-chip microlens, which is located in the sample. the side of the flow channel, and the optical axis of the on-chip microlens is perpendicular to the sample flow channel, for collimating the divergent light emitted by the sample in the sample flow channel into a polychromatic parallel light for output;

A second groove is formed on the chip bottom plate, and the second groove is triangular to construct an on-chip prism, the on-chip prism is located on the side of the on-chip microlens facing away from the sample flow channel, and is used for The polychromatic parallel light is dispersed into a spectrum composed of monochromatic light of different wavelengths and imaged on the edge of the chip substrate.

According to the microfluidic chip provided by the present invention, the optical axis of the on-chip prism coincides with the optical axis of the on-chip microlens.

According to a microfluidic chip provided by the present invention, the on-chip prism includes a first on-chip prism and a second on-chip prism arranged side by side, and the first on-chip prism is located on the on-chip microlens and the second on-chip prism. Between the prisms, the second on-chip prism is located between the first on-chip prism and the edge of the chip bottom plate.

According to a microfluidic chip provided by the present invention, the divergence angle K of the divergent light emitted by the sample in the sample flow channel satisfies:

and,

K=2a;

Among them, a is the incident angle of the divergent light passing through the sample flow channel and approaching the side of the on-chip microlens; n is the refractive index of the dielectric material of the chip bottom plate; b is the divergent light passing through the sample flow channel and approaching the on-chip microlens. Refraction angle at the side of the on-chip microlens; n ₁ is the refractive index of the sample medium; s ₁ is the maximum distance between the first arc surface and the side of the sample flow channel close to the on-chip microlens; R ₁ is The radius of curvature of the first arc surface; g is the angle formed between the intersection point of the diverging light and the first arc surface and the connecting line of the center of curvature of the first arc surface and the optical axis; l ₂ is the diverging light The distance from the intersection point with the first arc surface to the optical axis; s is the distance between the sample and the side surface of the sample flow channel close to the on-chip microlens.

According to a microfluidic chip provided by the present invention, when the chip bottom plate is made of glass material:

0.2mm≤R ₁ ≤3mm, 0.2mm≤R ₂ ≤3mm;

0.2mm≤s ₁ ≤5mm, 0.2≤s ₂ ≤5mm, 0.2≤s ₃ ≤5mm;

Wherein, R ₂ is the radius of curvature of the second arc surface; s ₂ is the minimum distance between the first arc surface and the second arc surface; s ₃ is the second arc surface and the first arc surface The maximum distance between the intersection of the first dispersion slope of the upper prism and the optical axis; l3 is the distance from the highest end point _of the second arc surface to the optical axis.

According to a microfluidic chip provided by the present invention, the distance y between the dispersion position and the optical axis of the monochromatic light of different wavelengths imaged on the edge of the chip base plate satisfies:

When μ<(γ+δ), y=(y ₃ ·tanδ+x ₄ )·tan(δ-ρ)+y ₃ ;

When μ>(γ+δ), y=(y ₃ ·tanδ+x ₄ )·tan(δ+ρ)+y ₃ ;

Among them, μ is the refraction angle of the monochromatic light passing through the third dispersion slope of the prism on the second sheet; γ is the angle between the third dispersion slope of the prism on the second sheet and the line perpendicular to the optical axis; δ is the The angle between the 4th dispersion inclined plane of the prism on the second sheet and the straight line perpendicular to the optical axis; y is the distance from the intersection of the 4th dispersion inclined plane _of the monochromatic light and the prism on the second sheet to the optical axis; x ₄ is The distance between the intersection of the fourth dispersion slope and the optical axis to the edge surface of the chip base plate; ρ is the refraction angle of the monochromatic light when it passes through the fourth dispersion slope of the second upper prism;

And the dispersion broadening of the spectrum is: the y value of the minimum wavelength monochromatic light minus the y value of the maximum wavelength monochromatic light.

According to a microfluidic chip provided by the present invention, when the chip bottom plate is made of glass material, the dispersion broadening range of the spectrum is 1-30 mm.

According to a microfluidic chip provided by the present invention, when the chip bottom plate is made of glass material:

0.2mm≤s ₃ ≤5mm;

0°≤α≤40°; 0°≤β≤45°; -70°≤γ≤5.7°; 11°≤δ≤75°;

0.1mm≤x ₁ ≤3mm; 0.1mm≤x ₂ ≤3mm;

0.1mm≤x ₃ ≤3mm; 0.1mm≤x ₄ ≤30mm;

Wherein, s ₃ is the maximum distance between the second arc surface and the intersection of the first dispersion slope of the first upper prism and the optical axis; α is the polychromatic parallel light emitted by the second arc surface passing through the The incident angle of the first dispersive slope of the first on-chip prism or the angle between the first dispersive slope of the first on-chip prism and the straight line perpendicular to the optical axis; β is the second The angle between the dispersion slope and the straight line perpendicular to the optical axis; x ₁ is the distance from the intersection of the first dispersion slope and the optical axis to the intersection of the second dispersion slope and the optical axis; x ₂ is the second dispersion slope The distance from the intersection with the optical axis to the intersection of the third dispersion slope and the optical axis; x ₃ is the distance from the intersection of the third dispersion slope and the optical axis to the intersection of the fourth dispersion slope and the optical axis.

A microfluidic chip provided according to the present invention further comprises a transparent chip top cover, the chip top cover is sealed on the chip bottom plate, and the chip top cover is provided with a sample inlet hole and a sample outlet hole, The sample injection hole and the sample outlet hole are in corresponding communication with both ends of the sample flow channel.

The present invention also provides a spectrum information detection system, comprising: a spectrum detection device and the above-mentioned microfluidic chip, wherein the spectrum detection device is used to detect the spectrum.

The microfluidic chip and the spectral information detection system provided by the present invention can obtain the spectrum composed of monochromatic light of different wavelengths by integrating the sample flow channel, the on-chip microlens and the on-chip prism on the microfluidic chip, so as to facilitate the follow-up Multiple fluorophores with highly overlapping emission spectra can be simultaneously and continuously detected by the spectral detection device to obtain high resolution; and the optical component module is simplified, the volume of the detection system is greatly reduced, the portability is convenient and the production cost is reduced, so that the The spectral information detection system based on the microfluidic chip is suitable for various real-time detection occasions. For different samples to be tested, suitable materials are selected and a unique chip structure is designed, which can effectively improve the detection stability and detection accuracy. Diversified clinical information; at the same time, the designed fixed optical element structure (on-chip microlens and on-chip prism) ensures the accuracy of the optical system and the stability of detection; the present invention integrates the on-chip microlens and on-chip prism on a chip, The optimal position can be designed for different applications to ensure that more effective optical signals are collected, the loss of optical signals is reduced, and the detection accuracy is improved. Therefore, the present invention realizes the spectral signal detection of the sample to be tested by designing and processing an integrally formed microfluidic chip, and combining the chip with the spectral detection device, which provides an innovative method for the application of the microfluidic chip in the field of real-time detection. It is of great significance especially for the spectral detection of clinical infectious diseases and cardiovascular diseases.

Additional aspects and advantages of the present invention will be set forth, in part, from the following description, and in part will be apparent from the following description, or may be learned by practice of the invention.

Description of drawings

In order to illustrate the technical solutions in the present invention or related technologies more clearly, the following briefly introduces the accompanying drawings required in the description of the embodiments or related technologies. Obviously, the drawings in the following description are some of the present invention. In the embodiments, for those of ordinary skill in the art, other drawings can also be obtained according to these drawings without any creative effort.

1 is a schematic structural diagram of a chip base plate provided by the present invention;

2 is a schematic structural diagram of a microfluidic chip provided by the present invention;

3 is a schematic diagram of an optical path of an on-chip microlens provided by the present invention;

Fig. 4 is the optical path schematic diagram of the on-chip prism provided by the present invention;

5 is a schematic structural diagram of a spectral information detection system provided by the present invention;

Reference number:

100: microfluidic chip; 101: chip bottom plate; 1001: sample flow channel;

1002: first arc surface; 1003: second arc surface; 1004: on-chip microlens;

1005: Optical axis; 1006: Prism on the first sheet; 1007: Prism on the second sheet;

1008: the first dispersion slope; 1009: the second dispersion slope;

1010: the third dispersion slope; 1011: the fourth dispersion slope; 1012: the edge surface;

102: chip top cover; 1021: injection hole; 1022: sample outlet;

200: sample syringe; 300: array photodetector; 400: upper computer;

500: collection bucket; 600: sample.

Detailed ways

In order to make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions in the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present invention. , not all examples. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

In the description of the embodiments of the present invention, it should be noted that the terms "center", "portrait", "horizontal", "top", "bottom", "front", "rear", "left", "right" , "vertical", "horizontal", "top", "bottom", "inside", "outside", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, only for the convenience of describing this Inventive embodiments and simplified descriptions are not intended to indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operate in a particular orientation, and therefore should not be construed as limiting the embodiments of the present invention. Furthermore, the terms "first," "second," etc. are used for descriptive purposes only and should not be construed to indicate or imply relative importance.

In the description of the embodiments of the present invention, it should be noted that, unless otherwise expressly specified and limited, the terms "connected" and "connected" should be understood in a broad sense, for example, it may be a fixed connection or a detachable connection, Or integral connection; it can be mechanical connection or electrical connection; it can be directly connected or indirectly connected through an intermediate medium. Those of ordinary skill in the art can understand the specific meanings of the above terms in the embodiments of the present invention in specific situations.

In the embodiments of the present invention, unless otherwise expressly specified and limited, the first feature "above" or "under" the second feature may be in direct contact with the first and second features, or the first and second features pass through the middle indirect contact with the media. Also, the first feature being "above", "over" and "above" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is level higher than the second feature. The first feature being "below", "below" and "below" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature has a lower level than the second feature.

In the description of this specification, description with reference to the terms "one embodiment," "some embodiments," "example," "specific example," or "some examples", etc., mean specific features described in connection with the embodiment or example , structures, materials, or features are included in at least one example or example of embodiments of the present invention. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, those skilled in the art may combine and combine the different embodiments or examples described in this specification, as well as the features of the different embodiments or examples, without conflicting each other.

The microfluidic chip and the spectral information detection system of the present invention will be described below with reference to FIGS. 1 to 5 .

According to the embodiment of the first aspect of the present invention, referring to FIGS. 1-4 , the microfluidic chip 100 provided by the present invention mainly includes a transparent chip base plate 101 , that is, the chip base plate 101 is made of a transparent material, and the chip base plate 101 is made of a transparent material. A sample flow channel 1001 is formed on the sample flow channel 101. The sample flow channel 1001 can realize the loading and flow of the sample 600 and play a role in restricting the flow of the sample 600. The sample flow channel 1001 is the sample 600, and the outside of the sample flow channel 1001 is the chip bottom plate 101 material. The medium, the characteristic size of the sample flow channel 1001 can be matched with the actual application of the sample 600 , for example, the aspect ratio of the sample flow channel 1001 can be designed according to the test requirements of the actual sample 600 . It can be understood that the specific type of the sample 600 of the present invention is not particularly limited, as long as it can generate a spectral signal, for example, it can be a cell, a suspension, and the like.

A first groove is formed on the chip base plate 101, and the first groove includes two relatively concave first arc surfaces 1002 and second arc surfaces 1003 to construct an on-chip microlens 1004, that is, the first groove is called an on-chip microlens. The lens 1004, between the first arc surface 1002 and the second arc surface 1003 (that is, inside the first groove) can be an air medium or other medium that conforms to the law of light transmission, and the outside of the first groove is a material medium of the chip bottom plate 101; The on-chip microlens 1004 is located on the side of the sample flow channel 1001, and the optical axis 1005 of the on-chip microlens 1004 is perpendicular to the sample flow channel 1001, and is used for collimating the divergent light emitted by the sample 600 in the sample flow channel 1001 into a polychromatic parallel light output .

A second groove is formed on the chip base plate 101, and the second groove is triangular to construct an on-chip prism, that is, the second groove is called an on-chip prism, and the inside of the second groove can be an air medium or a light-dispersion principle. Other medium; the outside of the second groove is the material medium of the chip bottom plate 101; the on-chip prism is located on the side of the on-chip microlens 1004 facing away from the sample flow channel 1001, and is used to disperse the polychromatic parallel light formed by the collimation of the on-chip microlens 1004 into Spectra composed of monochromatic light of different wavelengths, and the spectrum is imaged on the edge of the chip substrate 101 .

In the microfluidic chip 100 provided by the embodiment of the present invention, by integrally integrating the sample flow channel 1001, the on-chip microlens 1004 and the on-chip prism on the microfluidic chip 100, a spectrum composed of monochromatic light of different wavelengths can be obtained, so as to facilitate the Subsequently, multiple fluorophores with highly overlapping emission spectra can be simultaneously detected to obtain higher resolution; and the loss of optical signals can be reduced, the detection accuracy can be improved, and it has the advantages of simple structure, small size, low cost, portability, and stable reliability. It is of great significance for the spectral detection of clinical infectious diseases and cardiovascular diseases, etc.

According to an embodiment of the present invention, as shown in FIG. 2 , the microfluidic chip 100 of the present invention further includes a transparent chip top cover 102 , that is, the chip top cover 102 is made of a transparent material, and the chip top cover 102 is sealed and disposed on the chip bottom plate 101 , and the chip top cover 102 is provided with a sample injection hole 1021 and a sample outlet hole 1022 . The sample 600 enters the sample flow channel 1001 through the sample injection hole 1021 and emits divergent light, which is collimated by the on-chip microlens 1004 and dispersed into a spectrum by the on-chip prism in turn, and then flows out through the sample outlet hole 1022 .

In this example, the sample flow channel 1001 , the on-chip microlens 1004 and the on-chip prism are all integrally formed on the upper surface of the chip base plate 101 , the chip top cover 102 is sealed and stacked on the upper surface of the chip base plate 101 , and the sample injection holes 1021 and the outlet The sample holes 1022 are respectively aligned with both ends of the sample flow channel 1001 .

The specific materials of the microfluidic chip 100 of the present invention are not particularly limited, and transparent materials with excellent optical properties (such as quartz, glass, PDMS, PMMA, SU-8 photoresist, etc.) can be used to process the chip bottom plate 101 and the chip top cover. 102.

According to an embodiment of the present invention, the sample flow channel 1001 has a width of 100 microns and a depth of 200 microns.

According to an embodiment of the present invention, as shown in FIG. 1 , the optical axis 1005 of the on-chip prism coincides with the optical axis 1005 of the on-chip microlens 1004, which is beneficial to the design and processing of microstructures, and ensures the accuracy and stability of the on-chip optical propagation process. Reduce the error interference introduced by background light or material medium, so that the dispersion effect can be improved and the accuracy of spectral detection can be improved.

According to an embodiment of the present invention, as shown in FIG. 1 and FIG. 4 , the on-chip prism includes a first on-chip prism 1006 and a second on-chip prism 1007 arranged side by side, and the first on-chip prism 1006 is located between the on-chip microlens 1004 and the second on-chip prism 1007 . Between the on-chip prisms 1007 , the second on-chip prism 1007 is located between the first on-chip prism 1006 and the edge of the chip substrate 101 . Specifically, the first on-chip prism 1006 has two dispersion slopes, which are the first dispersion slope 1008 and the second dispersion slope 1009 respectively; the second on-chip prism 1007 has two dispersion slopes, which are the third dispersion slope 1010 and the fourth dispersion slope respectively. Dispersion slope 1011; and the optical axes 1005 of the first on-chip prism 1006 and the second on-chip prism 1007 coincide with the optical axis 1005 of the on-chip microlens 1004; the air medium is between the two dispersive slopes of each on-chip prism.

It can be understood that the specific number of on-chip prisms in the present invention is not particularly limited, and can be adjusted according to actual design requirements.

Since the on-chip microlens 1004 of the present invention is mainly used to collimate the divergent light emitted by the sample 600 into a polychromatic parallel light for output, therefore, in order to collect a suitable divergent light signal, it is necessary to measure the divergence angle K of the divergent light emitted by the sample 600 . Calculation and selection of appropriate design parameters can effectively improve detection stability and detection accuracy.

According to an embodiment of the present invention, as shown in FIG. 3 , the center of curvature of the first arc surface 1002 of the on-chip microlens 1004 is point O, and the center of curvature of the second arc surface 1003 of the on-chip microlens 1004 is point O' , a is the incident angle when the divergent light passes through the sample flow channel 1001 and is close to the side of the on-chip microlens 1004; n is the refractive index of the dielectric material of the chip base plate 101; n ₁ is the refractive index of the medium of the sample 600; s ₁ is the maximum distance between the first arc surface 1002 and the side surface of the sample flow channel 1001 close to the on-chip microlens 1004; R ₁ is the radius of curvature of the first arc surface 1002 g is the angle formed by the line of intersection B of the divergent light and the first arc surface 1002 and the center of curvature O of the first arc surface 1002 and the optical axis 1005; l ₂ is the intersection of the diverging light and the first arc surface 1002 The distance from point B to the optical axis 1005; s is the distance between the sample 600 and the side surface of the sample flow channel 1001 close to the on-chip microlens 1004; R ₂ is the radius of curvature of the second arc surface 1003; s ₂ is the first arc surface 1002 The minimum distance between the second arc surface 1003 and the second arc surface 1003; s ₃ is the maximum distance between the intersection point between the second arc surface 1003 and the first dispersion slope 1008 of the prism 1006 on the first sheet and the optical axis 1005; l ₃ is the second arc The distance from the highest end point C of the surface 1003 to the optical axis.

In addition, the refractive index of the air medium between the first arc surface 1002 and the second arc surface 1003 is 1, and the light parallel to the first dispersive slope 1008 of the first on-chip prism 1006 passes through the second arc surface 1003 of the on-chip microlens 1004 When the highest end point C of , the incident angle is e, and the refraction angle is f; when the light passes through the first arc surface 1002 of the on-chip microlens 1004, the incident angle is c, and the refraction angle is d, and the light intersects the side of the sample flow channel 1001 at Point A, the vertical distance from point A to the optical axis 1005 is set to l ₁ .

make

At this time there is

l ₂ and g can be obtained by transformation;

order again

d=g+f-e; (5)

b=g-c; (7)

get

and also have

By adjusting the values of the independent variables R ₁ , R ₂ , s ₁ , s ₂ , and l ₃ , a suitable dependent variable a can be obtained according to formula (8) and formula (9), so as to satisfy the requirements of the sample 600 issued in the actual detection process. The divergence angle K=2a of divergent light is required, and then a suitable divergent light signal is collected.

According to an embodiment of the present invention, when the chip bottom plate 101 is made of glass:

0.2mm≤R ₁ ≤3mm, 0.2mm≤R ₂ ≤3mm;

0.2mm≤s ₁ ≤5mm, 0.2≤s ₂ ≤5mm, 0.2≤s ₃ ≤5mm;

Through the above parameter design, the embodiment of the present invention can obtain spectral information with higher resolution on the premise of ensuring the small volume of the entire chip, which is conducive to the simultaneous detection of multiple fluorophores with highly overlapping emission spectra.

Because the on-chip prism of the present invention is mainly used to disperse the polychromatic parallel light formed by the collimation of the on-chip microlens 1004 into spectral information composed of monochromatic light of different wavelengths, and image the edge of the microfluidic chip 100 . In order to obtain the dispersion broadening that meets the detection requirements of the spectral detection device, it is necessary to calculate the dispersion position of monochromatic light of different wavelengths, and select appropriate design parameters, which is beneficial to improve the detection accuracy.

According to an embodiment of the present invention, as shown in FIG. 4 , where μ is the refraction angle of the monochromatic light passing through the third dispersion slope 1010 of the second upper prism 1007 ; γ is the third dispersion slope of the second upper prism 1007 The angle between 1010 and the line perpendicular to the optical axis 1005; δ is the angle between the fourth dispersion slope 1011 of the prism 1007 on the second sheet and the straight line perpendicular to the optical axis 1005; y ₃ is the angle between the monochromatic light and the prism 1007 on the second sheet The distance from the intersection point F of the four dispersion slopes 1011 to the optical axis 1005; x ₄ is the distance from the intersection of the fourth dispersion slope 1011 and the optical axis 1005 to the edge surface 1012 of the chip bottom plate 101; ρ is the distance between the monochromatic light passing through the second Refraction angle of the fourth dispersion slope 1011 of the on-chip prism 1007; y is the distance between the dispersion position and the optical axis 1005 of the monochromatic light of different wavelengths imaged on the edge of the chip substrate 101 .

折射角为μ，并且折射光线与第三色散斜面1010相交于点E，点E到光轴1005的距离为y₂；折射光线经过第二片上棱镜1007的第四色散斜面1011时的入射角为π，折射角为ρ；β为第一片上棱镜1006的第二色散斜面1009与垂直于光轴1005直线的夹角；折射光线到达微流控芯片100的边缘面1012时相交于点G，点G与光轴1005之间的距离为y；x₁为第一色散斜面1008与光轴1005的交点至第二色散斜面1009与光轴1005的交点的距离；x₂为第二色散斜面1009与光轴1005的交点至第三色散斜面1010与光轴1005的交点的距离；x₃为第三色散斜面1010与光轴1005的交点至第四色散斜面1011与光轴1005的交点的距离。可以理解的是，图4中仅示出一条单色光进行示例说明，其他单色光同理。In addition, the refractive index of the air medium between the first dispersion slope 1008 and the second dispersion slope 1009 and between the third dispersion slope 1010 and the fourth dispersion slope 1011 is 1; s ₃ is the second arc surface 1003 and the first surface The maximum distance between the intersection of the first dispersion slope 1008 of the prism 1006 and the optical axis 1005; α is the incident angle of the polychromatic parallel light emitted by the second arc surface 1003 passing through the first dispersion slope 1008 of the prism 1006 on the first sheet or The angle between the first dispersive slope 1008 of the prism 1006 on the first sheet and the straight line perpendicular to the optical axis 1005, that is, the two angle values are equal, and ε is the polychromatic parallel light emitted by the second arc surface 1003 passing through the first sheet. The refraction angle of the first dispersion slope 1008 of the prism 1006, the incident angle of the light passing through the second dispersion slope 1009 of the first on-chip prism 1006 is ∈, the refraction angle is θ, and the refracted light intersects the second dispersion slope 1009 at Point D, the vertical distance from point D to the optical axis 1005 is y ₁ ; the incident angle of the light passing through the third dispersion slope 1010 of the second prism 1007 is

The refraction angle is μ, and the refracted ray intersects the third dispersive slope 1010 at point E, and the distance from point E to the optical axis 1005 is y ₂ ; the incident angle of the refracted ray passing through the fourth dispersive slope 1011 of the second prism 1007 is π, the refraction angle is ρ; β is the included angle between the second dispersion slope 1009 of the prism 1006 on the first sheet and the straight line perpendicular to the optical axis 1005; the refracted light intersects at point G when it reaches the edge surface 1012 of the microfluidic chip 100, The distance between point G and the optical axis 1005 is y; x ₁ is the distance from the intersection of the first dispersion slope 1008 and the optical axis 1005 to the intersection of the second dispersion slope 1009 and the optical axis 1005 ; x ₂ is the second dispersion slope 1009 The distance from the intersection with the optical axis 1005 to the intersection of the third dispersion slope 1010 and the optical axis 1005 ; x ₃ is the distance from the intersection of the third dispersion slope 1010 and the optical axis 1005 to the intersection of the fourth dispersion slope 1011 and the optical axis 1005 . It can be understood that only one piece of monochromatic light is shown in FIG. 4 for illustration, and the same is true for other monochromatic lights.

Have

ε=arcsin(n·sinα); (10)

When ε<(α+β)

∈=α+β-ε; (11)

When ε>(α+β)

∈=ε-α-β; (17)

When μ<(γ+δ)

π=γ+δ-μ; (23)

y=(y ₃ ·tanδ+x ₄ )·tan(δ-ρ)+y ₃ ; (26)

When μ>(γ+δ)

π = μ-γ-δ; (27)

y=(y ₃ ·tanδ+x ₄ )·tan(δ+ρ)+y ₃ ; (30)

By designing appropriate independent variables α, β, γ, δ, x ₁ , x ₂ , x ₃ , x ₄ , according to formula (26) and formula (30), it is possible to calculate the edge surface 1012 of the microfluidic chip for light with different wavelengths The distance y between the corresponding dispersion position and the optical axis 1005; and the appropriate spectral dispersion broadening can be obtained by subtracting the y value calculated by the minimum wavelength monochromatic light from the y value calculated by the maximum wavelength monochromatic light.

According to an embodiment of the present invention, when the chip base plate 101 is made of glass, the spectral dispersion broadening range is 1-30 mm, which can improve the dispersion effect and improve the detection accuracy of the spectrum.

According to an embodiment of the present invention, when the chip bottom plate 101 is made of glass:

0.2mm≤s ₃ ≤5mm;

0°≤α≤40°; 0°≤β≤45°; -70°≤γ≤5.7°; 11°≤δ≤75°;

0.1mm≤x ₁ ≤3mm; 0.1mm≤x ₂ ≤3mm;

_{0.1mm≤x3≤3mm} ; _{0.1mm≤x4≤30mm} .

Through the above parameter design in the embodiment of the present invention, the volume of the entire chip can be ensured to be small, and spectral information with higher resolution can be obtained, which is beneficial to improve the detection accuracy.

According to an embodiment of the second aspect of the present invention, the present invention further provides a spectral information detection system, comprising: a spectral detection device and the microfluidic chip 100 of the above-mentioned embodiment, the spectral detection device is used for detecting and imaging on the microfluidic chip 100 on the spectral signal.

The specific type of the spectral detection device of the present invention is not particularly limited, for example, various samples can be analyzed for spectral signal detection, including but not limited to the analysis of spectral signals such as scattering spectra, fluorescence spectra, and chemiluminescence of the samples.

According to an embodiment of the present invention, as shown in FIG. 5 , the spectral detection device includes an array photodetector 300 and a host computer 400. The array photodetector 300 is mounted on the edge of the microfluidic chip 100 to realize continuous detection of spectral signals For acquisition, the host computer 400 is connected to the array photodetector 300 for analyzing the spectral information.

According to an embodiment of the present invention, the spectral information detection system further includes a sample injector 200 and a collection barrel 500. The sample injector 200 is connected to the injection hole 1021 of the chip top cover 102 through a conduit for injecting the sample 600 from the injection hole 1021. The sample flow channel 1001; the collection bucket 500 is connected to the sample outlet hole 1022 of the chip top cover 102 through a conduit, and the detected sample 600 can flow into the collection bucket 500 through the sample outlet hole 1022 for collection.

The working principle of the spectral information detection system provided by the present invention is described below with reference to a specific example, which roughly includes:

The suspension sample is injected from the injection hole 1021 into the sample flow channel 1001 through the sample injector 200. When the suspension sample flows through the specific detection position where the sample flow channel 1001 intersects the optical axis 1005, the suspension sample containing spectral signals will emit different The spectral signal of the wavelength is emitted at a certain divergence angle; the divergent light is collimated by the on-chip microlens 1004 into a polychromatic parallel light and enters the on-chip prism. Here, one or more on-chip prisms can be designed as required to obtain the best spectral broadening effect. . Under the dispersion effect of the on-chip prism, optical signals of different wavelengths are emitted to the edge of the microfluidic chip 100 at different angles to form dispersion broadening that meets the requirements of the array photodetector 300; then the array photodetector 300 detects and collects corresponding spectral information , and transmitted to the host computer 400 to present the spectral signal, so as to analyze the spectral information of the suspension sample;

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, but not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that it can still be The technical solutions described in the foregoing embodiments are modified, or some technical features thereof are equivalently replaced; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims (10)

1. A microfluidic chip, comprising:

a transparent chip base plate, wherein a sample flow channel is formed on the chip base plate;

a first groove is formed in the chip bottom plate, the first groove comprises a first arc surface and a second arc surface which are relatively recessed so as to construct an on-chip micro lens, the on-chip micro lens is located on the side surface of the sample flow channel, and the optical axis of the on-chip micro lens is perpendicular to the sample flow channel and is used for collimating divergent light emitted by a sample in the sample flow channel into polychromatic parallel light to be emitted;

the chip bottom plate is formed with a second groove, the second groove is triangular to construct an on-chip prism, the on-chip prism is located on one side of the on-chip micro lens, which faces away from the sample flow channel, and is used for dispersing the compound color parallel light into a spectrum composed of monochromatic light with different wavelengths and imaging the spectrum on the edge of the chip bottom plate.

2. The microfluidic chip of claim 1, wherein an optical axis of the on-chip prism coincides with an optical axis of the on-chip microlens.

3. The microfluidic chip according to claim 2, wherein the on-chip prisms include a first on-chip prism and a second on-chip prism arranged side by side, the first on-chip prism being located between the on-chip micro-lens and the second on-chip prism, the second on-chip prism being located between the first on-chip prism and an edge of the chip base plate.

4. The microfluidic chip according to claim 3, wherein the divergent angle K of the divergent light emitted by the sample in the sample channel satisfies the following condition:

and the number of the first and second electrodes,

K＝2a；

wherein a is an incident angle when divergent light passes through the side surface of the sample flow channel close to the on-chip micro lens; n is the refractive index of the dielectric material of the chip bottom plate;b is the refraction angle of the divergent light passing through the side surface of the sample flow channel close to the on-chip micro lens; n is ₁ Is the sample medium refractive index; s ₁ The maximum distance between the first cambered surface and the side surface of the sample flow channel close to the on-chip micro lens is set; r ₁ The curvature radius of the first cambered surface; g is an included angle formed by a connecting line of an intersection point of the divergent light and the first cambered surface and a curvature circle center of the first cambered surface and an optical axis; l ₂ The distance from the intersection point of the divergent light and the first cambered surface to the optical axis; and s is the distance between the sample and the side surface of the sample flow channel close to the on-chip micro lens.

5. The microfluidic chip according to claim 4, wherein when the chip bottom plate is made of glass:

0.2mm≤R ₁ ≤3mm，0.2mm≤R ₂ ≤3mm；

0.2mm≤s ₁ ≤5mm，0.2≤s ₂ ≤5mm，0.2≤s ₃ ≤5mm；

wherein R is ₂ The radius of curvature of the second cambered surface; s ₂ Is the minimum distance between the first cambered surface and the second cambered surface; s ₃ The maximum distance between the second cambered surface and the intersection point of the first dispersion inclined plane and the optical axis of the first upper prism is set; l ₃ The distance from the highest end point of the second cambered surface to the optical axis.

6. The microfluidic chip according to claim 3, wherein the distance y between the dispersion position of monochromatic light with different wavelengths imaged on the edge of the chip bottom plate and the optical axis satisfies:

when mu is<(γ+δ)，y＝(y ₃ ·tanδ+x ₄ )·tan(δ-ρ)+y ₃ ；

When mu is>(γ+δ)，y＝(y ₃ ·tanδ+x ₄ )·tan(δ+ρ)+y ₃ ；

Mu is a refraction angle when monochromatic light passes through the third color dispersion inclined plane of the second on-chip prism; gamma is an included angle between the third astigmatism inclined plane of the second on-chip prism and a straight line vertical to the optical axis; delta is an included angle between a fourth dispersion inclined plane of the second on-chip prism and a straight line vertical to the optical axis; y is ₃ The distance from the intersection point of the monochromatic light and the fourth dispersion inclined plane of the second on-chip prism to the optical axis; x is the number of ₄ The distance from the intersection point of the fourth dispersion slope and the optical axis to the edge surface of the chip bottom plate is obtained; rho is a refraction angle of the monochromatic light when the monochromatic light passes through the fourth dispersion slope of the second on-chip prism;

and the dispersion spread of the spectrum is: the y value of the minimum wavelength monochromatic light minus the y value of the maximum wavelength monochromatic light.

7. The microfluidic chip according to claim 6, wherein when the bottom plate is made of glass, the dispersion broadening range of the spectrum is 1-30 mm.

8. The microfluidic chip according to claim 6, wherein when the chip bottom plate is made of glass:

0.2mm≤s ₃ ≤5mm；

0°≤α≤40°；0°≤β≤45°；-70°≤γ≤5.7°；11°≤δ≤75°；

0.1mm≤x ₁ ≤3mm；0.1mm≤x ₂ ≤3mm；

0.1mm≤x ₃ ≤3mm；0.1mm≤x ₄ ≤30mm；

wherein s is ₃ The maximum distance between the second cambered surface and the intersection point of the first dispersion inclined plane and the optical axis of the first upper prism is set; alpha is an incident angle when the multi-color parallel light emitted from the second cambered surface passes through the first dispersion slope of the first upper prism or an included angle between the first dispersion slope of the first upper prism and a straight line perpendicular to the optical axis; beta is an included angle between the second dispersion inclined plane of the first upper prism and a straight line vertical to the optical axis; x is the number of ₁ Is from the intersection point of the first dispersion slope and the optical axis to the secondDistance of intersection point of dispersion slope and optical axis; x is the number of ₂ The distance from the intersection point of the second dispersion inclined plane and the optical axis to the intersection point of the third dispersion inclined plane and the optical axis is included; x is the number of ₃ The distance from the intersection point of the third dispersion slope and the optical axis to the intersection point of the fourth dispersion slope and the optical axis.

9. The microfluidic chip according to any of claims 1 to 8, further comprising a transparent chip top cap, wherein the chip top cap is hermetically disposed on the chip bottom plate, and the chip top cap is provided with a sample inlet and a sample outlet, and the sample inlet and the sample outlet are correspondingly communicated with two ends of the sample channel.

10. A system for detecting spectral information, comprising: a spectroscopic detection device for detecting the spectrum and a microfluidic chip according to any one of claims 1 to 9.

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