CN114887028A

CN114887028A - Prescription and capsule for repairing damaged taste cells

Info

Publication number: CN114887028A
Application number: CN202210491582.2A
Authority: CN
Inventors: 陈成; 刘添夫
Original assignee: Suzhou Nmt Biotech Co ltd
Current assignee: Suzhou Nmt Biotech Co ltd
Priority date: 2022-05-07
Filing date: 2022-05-07
Publication date: 2022-08-12

Abstract

The invention discloses a formula for repairing damaged taste cells, which comprises the following components in percentage: 25 to 30 percent of 5-aminolevulinic acid, 10 to 15 percent of ferrous glycinate, 0.05 to 0.15 percent of mecobalamin, 1 to 3 percent of oryzanol and 0.1 to 0.2 percent of cerebroprotein hydrolysate. The invention can treat the taste failure symptom caused by the novel coronavirus by adding 5-ALA and ferrous ions and three substances for repairing damaged nerve cells.

Description

Prescription and capsule for repairing damaged taste cells

Technical Field

The invention relates to a biomedical technology, in particular to a biomedical technology used in the field of recovering damaged cells, and specifically discloses a formula and a capsule for repairing damaged taste cells.

Background

Since twelve months 2019, the number of people infected with new coronaviruses has been more than four billion, with light and asymptomatic infections accounting for more than two thirds. After the common or light new coronary pneumonia patients are treated, most of the patients can be cured, the bodies have no obvious discomfort, and a few patients have slight or non-continuous lung function problems, such as lung fibrous tissue hyperplasia, lung tissue fibrosis, fatigue, muscle weakness, taste and smell failure and the like. Taste failure is the most common sequelae of new coronary pneumonia, patients are usually relieved after several weeks, and the cure speed of patients with taste failure can be accelerated by the treatment of medicinal means.

5-aminolevulinic acid is a substance naturally existing in a human body, and the supplementation of endogenous active substances in the human body by adding 5-aminolevulinic acid from an external source can also become an effective way for repairing damaged taste cells.

Therefore, there is a need for formulations and capsules for repairing damaged taste cells to achieve the above objectives.

Disclosure of Invention

It is an object of the present invention to provide a formulation for repairing damaged taste cells.

The technical scheme is as follows:

a formulation for repairing damaged taste cells, comprising, in percent:

25 to 30 percent of 5-aminolevulinic acid, 10 to 15 percent of ferrous glycinate, 0.05 to 0.15 percent of mecobalamin, 1 to 3 percent of oryzanol and 0.1 to 0.2 percent of cerebroprotein hydrolysate.

Furthermore, the compound comprises 25 to 30 percent of 5-aminolevulinic acid, wherein the 5-aminolevulinic acid is a substance existing in a human body, is a prefix compound of tetrahydropyrrole substances (tetrahydropyrrole is a substance forming heme, cytochrome and vitamin B12), and is an essential substance for the human body to synthesize heme, vitamin B12 and the like; 5-aminolevulinic acid can participate in the process of combining with cytochrome in an electron transfer chain of mitochondria in a human body, and can improve the functions of the mitochondria; the 5-aminolevulinic acid participates in the production process of adenosine triphosphate in a human body, can increase the energy of all cells, promotes the metabolism of the cells and accelerates the self-repair speed of damaged taste cells.

Furthermore, 10% -15% of ferrous glycinate is included, the ferrous glycinate is an amino acid chelate, and iron is a main component of hemoglobin, myoglobin and various enzyme systems and plays an important role in nutrition and immunity in vivo; the ferrous glycinate has the characteristics of good stability, easy absorption, no fishy smell, no gastrointestinal irritation, no toxic or side effect, high bioavailability and the like; ferrous glycinate can supplement ferrous ions exogenously, the ferrous ions can participate in the reaction of 5-aminolevulinic acid in a human body, and the exogenous supplement of the human endogenous substance 5-aminolevulinic acid is combined to form an endogenous substance and promote the substance to synchronously supplement and repair the damaged taste cells.

Furthermore, the oral liquid also comprises 0.05 to 0.15 percent of mecobalamin which is endogenous vitamin B12 and exists in blood and medullary fluid, compared with vitamin B12, the oral liquid has good improvement effect on the conduction of neurons, can promote nucleic acid-protein-fat metabolism through methyl conversion reaction, can be used as a coenzyme of methionine synthetase, can convert homocysteine into methionine, participates in the process of deoxynucleoside synthesizing thymine, promotes nucleic acid and protein synthesis, promotes axon internal transportation, axon regeneration and myelination, prevents axon mutability, and repairs damaged nervous tissues.

Furthermore, the rice oil contains 1% -3% of oryzanol, wherein the oryzanol is a mixture of ferulic acid ester which is present in rice oil and mainly contains triterpene (alkene) alcohol, mainly acts on the autonomic nervous system and the endocrine center of diencephalon, can adjust autonomic nervous function, reduce endocrine balance disorder and improve mental nerve disorder symptoms, and also has the effect of repairing damaged nervous tissues and nerve cells.

Further, 0.1% -0.2% of cerebroprotein hydrolysate is included, the cerebroprotein hydrolysate is an active peptide hydrolysate, is a nerve protective agent generated by hydrolyzing pig brain protease, contains ganglioside, can prevent brain cells from being damaged, and has the function of repairing damaged nerves; the brain protein hydrolysate contains nerve growth factor, and can maintain the neuron cell of central nervous system in survival state, promote the growth of nerve fiber, promote the growth of nerve cell, shorten its growth cycle, improve damaged nerve, and relieve cognitive dysfunction of patients.

Another object of the present invention is to provide a capsule for repairing damaged taste cells, which is prepared based on a formulation for repairing damaged taste cells of one of the objects.

Compared with the prior art, the invention can treat the taste failure symptom caused by the novel coronavirus by adding the 5-ALA and the ferrous ions and three substances for repairing damaged nerve cells.

Drawings

FIG. 1 is a table of death data of acute oral toxicity test of rats according to example 2 of the present invention.

FIG. 2 is a table showing the body weight change of the rat acute oral toxicity test in example 2 of the present invention.

FIG. 3 is a table of taste test sample compositions of example 3 of the present invention.

FIG. 4 is a graphical representation of the taste restoration assay of the capsule for repairing damaged taste cells of example 3 of the present invention.

FIG. 5 is a table of taste sensitivity test sample compositions of example 4 of the present invention.

FIG. 6 is a chart of the taste sensitivity test recordings for the restoration of damaged taste cell capsules of example 4 of the present invention.

Detailed Description

Example 1:

this example shows a capsule for repairing damaged taste cells comprising, in percent:

25 to 30 percent of 5-aminolevulinic acid, 10 to 15 percent of ferrous glycinate, 0.05 to 0.15 percent of mecobalamin, 1 to 3 percent of oryzanol, 0.1 to 0.2 percent of cerebroprotein hydrolysate, 0.5 to 1 percent of compound mineral, 40 to 60 percent of pregelatinized starch, 0.1 to 0.5 percent of magnesium stearate, 0.5 to 2 percent of colloidal silicon dioxide and 3 to 5 percent of low-substituted hydroxypropyl methyl cellulose.

The specific content is not precisely limited in this embodiment, and the list is only one showing manner of the present solution.

5-aminolevulinic acid is an important amino acid existing in mitochondria in cells, is an important component for promoting energy generation, and is an effective substance for accelerating metabolism and increasing the cell generation speed;

the composition comprises 25-30% of 5-aminolevulinic acid, can be added with the 5-aminolevulinic acid from an external source, and the 5-aminolevulinic acid can participate in the process of combining with cytochrome in an electron transfer chain of mitochondria in a human body, so that the function of the mitochondria can be improved; the 5-aminolevulinic acid participates in the production process of adenosine triphosphate in a human body, can increase the energy of all cells, promote the metabolism of the cells and accelerate the speed of repairing the self-repair of damaged taste cells.

Comprises 10 to 15 percent of ferrous glycinate, ferrous glycinate can supplement ferrous ions from external sources, and the ferrous ions can participate in the reaction of the 5-aminolevulinic acid in the human body. The exogenous addition of ferrous glycinate can improve the effect of 5-aminolevulinic acid on repairing damaged taste cells in a human body.

Comprises 0.05-0.15% of mecobalamin, wherein the mecobalamin is endogenous vitamin B12, and the exogenous mecobalamin can improve the conduction of neurons, promote intraaxonal delivery, axonal regeneration and myelination, prevent axonal degeneration and repair damaged nerve tissues and cells.

Comprises 1-3% of oryzanol, wherein the oryzanol is a mixture of ferulic acid ester which exists in rice oil and takes triterpene (alkene) alcohol as a main body. The exogenous oryzanol can regulate autonomic nerve function, reduce endocrine balance disorder, improve mental disorder symptom, and repair damaged nerve tissue and nerve cells.

Comprises 0.1-0.2% of cerebroprotein hydrolysate, which is an active peptide hydrolysate and has significant biological activity. The exogenous addition of cerebroprotein hydrolysate can prevent brain cells from being damaged, repair damaged nerves, maintain the neuron cells of the central nervous system in a survival state, and promote the growth of nerve fibers.

The compound vitamin and the compound mineral are added as microelements for calming the nerves and aiding sleep, and the sleep quality and the sleep time are increased when the compound vitamin and the compound mineral are added to form auxiliary elements;

the compound vitamins include vitamin A, vitamin D, vitamin E, vitamin C, vitamin B1, vitamin B2, vitamin B6 and folic acid; the mass ratio of the vitamin A to the vitamin D to the vitamin E to the vitamin C to the vitamin B1 to the vitamin B2 to the vitamin B6 to the folic acid is 0.25-0.35: 0.01-0.05: 2.0-3.0: 0.40-0.50: 0.30-0.40: 0.15-0.20: 0.1-0.2: 0.20-0.40.

The vitamin composition also comprises 0.5-1% of compound vitamin, wherein the vitamin is a low molecular organic compound which is necessary for human metabolism and needs a small amount, can not be synthesized in vivo generally, is mainly provided by food, and most of the vitamin composition is required to be additionally added into the food except for individual vitamins. They do not provide energy and are not structural components of the body, but are absolutely indispensable in the diet, such as long-term deficiency or insufficiency of certain vitamins, which can cause metabolic disorders and pathological conditions to form vitamin deficiency;

the compound mineral comprises calcium lactate, copper gluconate, zinc gluconate, manganese gluconate and potassium iodide; the mass ratio of the calcium lactate to the copper gluconate to the zinc gluconate to the manganese gluconate to the potassium iodide is 0.50-0.60: 0.20-0.30: 0.45-0.50: 0.30-0.40.

The compound mineral composition also comprises 0.5-1% of compound minerals, wherein the minerals are important raw materials forming human body tissue and help to regulate acid-base balance, muscle contraction, nerve reaction and the like in vivo. The trace elements can provide necessary nutrients for human body, and can activate or inhibit certain vitamins, hormones and enzymes, so that the trace elements have very important function of ensuring normal physiological function and substance metabolism of human body, and have the functions of regulating organism metabolism, promoting recovery of damaged cells, promoting growth and development, enhancing disease resistance and the like.

Also comprises 40-60% of pregelatinized starch, wherein the pregelatinized starch is a modified starch obtained by breaking starch granules partially or completely by chemical or mechanical method. Pregelatinized starch is commonly used as a diluent in capsules to improve the physical properties of the contents and increase volume.

And 0.2-0.5% of magnesium stearate which is a hydrophobic lubricant is easy to be uniformly mixed with the granules and is often used for reducing the friction force between powder and metal materials in the preparation of capsules.

And 0.5-2% of colloidal silicon dioxide, wherein microcrystalline cellulose is commonly used as a glidant in capsule preparation to improve the flowability of contents.

The low-substituted hydroxypropyl methylcellulose is 2 to 5 percent and is often used as a disintegrant in capsule preparation, so that the low-substituted hydroxypropyl methylcellulose is favorable for disintegration and absorption of the capsule in a human body.

The preparation procedure is as follows:

1) mixing the raw materials thoroughly for 15 min;

2) fully sieving the mixed raw materials by a 100-mesh sieve;

3) putting the sieved raw materials into a batch feeder, and adding the raw materials into a hard capsule shell according to the amount to obtain the capsule;

4) the average weight of the capsule contents was 150mg, with a 5-aminolevulinic acid content of about 50 mg.

Example 2:

9 female rats were selected and divided into three ABC groups. 9 rats were fasted.

3 rats in group A are administered with the drug containing the formula of the invention at a dose of 300 mg/kg body weight, 6 rats in group BC are administered with the drug containing the formula of the invention at a dose of 2000 mg/kg body weight, two weeks are continuously observed after administration, and the death condition of the rats is recorded, and the test results are shown in figure 1;

referring to fig. 1:

three groups of ABC rats had no mortality status within two weeks of drug administration.

Observing and recording the body weight change condition of the ABC three groups of rats after the administration, wherein the test result is shown in figure 2;

referring to fig. 2:

three groups of ABC rats exhibited a normal increase in weight average in the body volume within two weeks of drug administration.

It can be seen by comparison that within two weeks of administration, no mortality was observed in all three groups of rats, no signs of systemic toxicity, all rats showed normal weight gain, and the acute oral median lethal dose should be greater than 2500 mg per kg of body weight.

Example 3:

40 patients with dysgeusia due to new coronary pneumonia were selected and divided into two groups AB, 20 individuals each.

Group A is placebo control group, and placebo containing no active substance is taken;

the group B is a medicine group, and the medicine containing the formula is taken;

the two groups AB are taken twice each time, three times a day, and the taking time lasts for one week.

After one week, performing taste test on the AB two groups, randomly selecting one group from the four groups of samples for tasting, after the taste of the tested person is remembered, disordering the five samples tasted by the tested person with the three blank samples, allowing the tested person to sample one by one again and selecting five tasteful samples, recording the number of people who successfully taste by the AB two groups of persons, referring to the sample composition table of FIG. 3, and obtaining the test result of FIG. 4;

referring to fig. 4:

the number of people who took group a with placebo and then restored their sense of taste was 2 after one week, and the number of people who took group B with the drug of the present formula and then restored their sense of taste was 16 after one week;

by comparison, it can be seen that the use of the capsule for repairing damaged taste cells of example 1 can significantly restore the condition of taste disturbance in patients.

Example 4:

One week later, performing taste sensitivity test on the AB groups, performing taste test on one of the four tastes by a tester, and passing the test if the concentration increase and decrease sequence is successfully judged according to the change of the concentration difference of the sample, wherein the sample composition table refers to FIG. 5, and the test result is shown in FIG. 6;

referring to FIG. 6:

the number of persons who took group a of placebo and 15 after one week who took the drug containing the formulation of the present invention and restored taste sensitivity was 1;

by comparison, it can be shown that the use of the capsule for repairing damaged taste cells of example 1 can significantly improve the sensitivity of taste in patients.

What has been described above are merely some embodiments of the present invention. It will be apparent to those skilled in the art that various changes and modifications can be made without departing from the inventive concept thereof, and these changes and modifications can be made without departing from the spirit and scope of the invention.

Claims

1. A formulation for repairing damaged taste cells, comprising: comprises the following components in percentage:

25 to 30 percent of 5-aminolevulinic acid, 10 to 15 percent of ferrous glycinate, 0.05 to 0.15 percent of mecobalamin, 1 to 3 percent of oryzanol and 0.1 to 0.2 percent of brain protein hydrolysate, wherein the brain protein hydrolysate is an active peptide neuroprotective agent generated by hydrolyzing porcine brain protease.

2. The formulation for repairing impaired taste cells of claim 1 wherein: comprises 25 to 30 percent of 5-aminolevulinic acid, wherein the 5-aminolevulinic acid is a substance existing in a human body, is a prefix compound of tetrahydropyrrole substances, and is an essential substance for the human body to synthesize heme, vitamin B12 and the like; 5-aminolevulinic acid can participate in the process of combining with cytochrome in an electron transfer chain of mitochondria in a human body, and can improve the functions of the mitochondria; the 5-aminolevulinic acid participates in the production process of adenosine triphosphate in human body, can increase the energy of all cells, promote the metabolism of the cells and accelerate the self-repair speed of the damaged taste cells.

3. The formulation of claim 2 for repairing impaired taste cells, wherein: the method comprises the following steps of preparing 10% -15% of ferrous glycinate, wherein the ferrous glycinate is an amino acid chelate and can provide stable and easily absorbed ferrous ions, the ferrous ions can participate in the reaction of 5-aminolevulinic acid in a human body, and the method combines an exogenous supplementary human endogenous substance 5-aminolevulinic acid to form an endogenous substance and promote the substance to synchronously supplement and repair damaged taste cells.

4. The formulation of claim 2 for repairing impaired taste cells, wherein: comprises 0.05-0.15% of mecobalamin, which is endogenous vitamin B12 existing in blood and medulla, and has good effect of improving neuron conduction and promoting nucleic acid-protein-fat metabolism through methyl conversion reaction; mecobalamin can promote nucleic acid and protein synthesis, promote axonal transport, axonal regeneration and myelin sheath formation, prevent axonal degeneration, and repair damaged nerve tissues.

5. The formulation of claim 2 for repairing impaired taste cells, wherein: comprises 1-3% of oryzanol, wherein the oryzanol is a mixture of ferulic acid ester which exists in rice oil and takes triterpene (alkene) alcohol as a main body, and the oryzanol mainly acts on the autonomic nervous system and the endocrine center of diencephalon, can adjust autonomic nervous function, reduce endocrine balance disorder and improve mental and neurological disorder symptoms; oryzanol has effects of repairing damaged nerve tissue and nerve cell.

6. The formulation of claim 2 for repairing impaired taste cells, wherein: comprises 0.1% -0.2% of cerebroprotein hydrolysate, which can prevent brain cells from being damaged and repair damaged nerves; the brain protein hydrolysate contains nerve growth factor, and can maintain the neuron cell of central nervous system in survival state, promote the growth of nerve fiber, promote the growth of nerve cell, shorten its growth cycle, improve damaged nerve, and relieve cognitive dysfunction of patients.

7. A capsule for repairing damaged taste cells, prepared based on the damaged taste cell repairing formulation of claim 2.