CN114859068A - Kit and POCT blood cell analyzer - Google Patents

Abstract

The application provides a kit and POCT blood cell analyzer. The kit comprises a kit body, a sealing ring, a microporous sheet and a rear tank body. The box body comprises a forebay and an installation cavity communicated with the forebay; the sealing ring sets up in the installation cavity, and is located the installation cavity and is close to one side of forebay, and the micropore piece is equipped with the micropore that allows the cell to pass through one by one, and the micropore piece sets up in the installation cavity, and is located the sealing ring and keeps away from one side of forebay, and back pond body butt is in the micropore piece and keeps away from one side of sealing ring for be fixed in on the box body with micropore piece and sealing ring, back pond body is formed with the drainage chamber, and forebay and drainage chamber pass through the micropore intercommunication. The application provides a simple structure of kit, easy assembly, and do benefit to the processing of kit.

Description

Kits and POCT Hematology Analyzers

technical field

The present application relates to the technical field of medical devices, in particular to a kit and a POCT blood cell analyzer.

Background technique

Hematology analyzer is a commonly used medical testing equipment. It is a kind of instrument that detects parameters such as the number and proportion of blood cells (red blood cells, white blood cells, platelets) in the blood. Diagnosis and treatment, blood disease diagnosis and other functions. With the advancement of technology and the development of science and technology, the function of the blood cell analyzer has been continuously expanded, the performance has been continuously improved, and the degree of automation has been continuously improved, and it has been widely used in clinical practice.

However, the installation of the microporous sheet in the existing POCT blood cell analyzer is relatively complicated, and there may be a risk of liquid leakage. In order to ensure its tightness and facilitate installation, the installation structure of the existing microporous sheet needs to be optimized.

SUMMARY OF THE INVENTION

The present application provides a kit and a POCT blood cell analyzer to solve the technical problems of the complex installation structure and poor sealing performance of the microporous sheet in the prior art.

In order to solve the above-mentioned technical problems, a technical solution adopted in the present application is: to provide a kit, the kit includes: a box body, including a forepool and an installation cavity communicated with the forepool; a sealing ring, arranged in the installation cavity, And it is located on the side of the installation cavity close to the fore tank. The micro-perforated sheet is provided with micro-holes that allow cells to pass through one by one. The pool body abuts on the side of the microporous sheet away from the sealing ring, and is used to fix the microporous sheet and the sealing ring on the box body.

Further, the box body includes a via hole that communicates with the front pool and the installation cavity, the ratio of the diameter of the microporous sheet to the diameter of the via hole is not less than 1.7, and the diameter of the microporous sheet is not larger than the inner diameter of the installation cavity.

Further, the sealing ring is in clearance fit with the installation cavity.

Further, the rear pool body is bonded to the box body, and the microporous sheet and the sealing ring are bonded to the box body. Alternatively, the rear pool body is bonded to the box body, and the microporous sheet and the sealing ring are bonded to the rear pool body.

Further, the outer end surface of the installation cavity is provided with a positioning protrusion, the rear pool body is provided with a positioning groove, and the positioning protrusion cooperates with the positioning groove to position the rear pool body, or the outer end surface of the installation cavity is provided with a positioning groove. The positioning groove is provided with a positioning protrusion on the rear pool body, and the positioning protrusion cooperates with the positioning groove to position the rear pool body.

Further, the front pool is provided with a front pool electrode, the back pool body is provided with a back pool electrode extending to the drainage cavity, the front pool electrode and the back pool electrode are located on both sides of the microporous sheet at intervals, and the inner end face of the back pool electrode is Convex.

Further, the back pool body includes a bottom wall and a side wall, the bottom wall is connected to the side wall to form a drainage cavity, the back pool electrode is arranged on the bottom wall, the side wall is provided with a liquid outlet, the liquid outlet is communicated with the drainage cavity, and the bottom is connected to the drainage cavity. The wall is a plane, the plane and the side wall are inclined, and the liquid outlet is arranged at the intersection of the bottom wall and the side wall, and is located on the side away from the microporous sheet.

Further, the back pool body comprises a bottom wall and a side wall, and the bottom wall connects the side walls to form a drainage cavity, and the back pool electrode is arranged on the bottom wall, and is provided with the position of the back pool electrode from the bottom wall in the direction of the edge of the bottom wall, The distance from the bottom wall to the microporous sheet gradually decreases.

Further, one side surface or both side surfaces of the microporous sheet is provided with a concave guide surface at the periphery of the microhole.

In order to solve the above-mentioned technical problems, another technical solution adopted in the present application is to provide a POCT blood cell analyzer, the POCT blood cell analyzer includes the kit of any of the above-mentioned embodiments and a detection seat matched with the kit, the POCT blood cell analyzer. The instrument is used for the analysis and detection of blood samples.

The beneficial effects of the present application are: different from the situation in the prior art, the kit of the present application includes a box body, a sealing ring, a microporous sheet and a back cell body, wherein the sealing ring, the microporous sheet, and the back cell body are sequentially arranged on the in the installation cavity of the box body, and the rear pool body is used to fix the microporous sheet and the sealing ring on the box body. The kit of the present application has a simple structure and is easy to assemble, and the fixing structure of the microporous sheet is novel, so that the sealing of the front end of the installation cavity is better, the risk of liquid leakage is reduced, and the reliability of the detection of the kit can be improved.

Description of drawings

In order to illustrate the technical solutions in the embodiments of the present application more clearly, the following briefly introduces the drawings that are used in the description of the embodiments. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, under the premise of no creative work, other drawings can also be obtained from these drawings, wherein:

1 is a schematic structural diagram of an embodiment of the kit provided by the application;

Fig. 2 is the exploded schematic diagram of the kit shown in Fig. 1;

3 is a schematic cross-sectional structure diagram of the kit shown in FIG. 1 from a perspective;

FIG. 4 is a schematic structural diagram of an embodiment of the rear tank body in the kit shown in FIG. 1;

5 is a schematic structural diagram of another embodiment of the back pool body in the kit shown in FIG. 1;

6 is a schematic structural diagram of another embodiment of the kit provided by the application;

Figure 7 is an exploded schematic view of the kit shown in Figure 6;

8 is a schematic structural diagram of another embodiment of the kit provided by the application;

Figure 9 is an exploded schematic view of the kit shown in Figure 8;

10 is a schematic structural diagram of another embodiment of the kit provided by the application;

FIG. 11 is a schematic structural diagram of an embodiment of a pipette and a pipette tip in the POCT blood cell analyzer provided by the present application.

Detailed ways

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, but not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

It should be noted that if there are directional indications (such as up, down, left, right, front, back, etc.) involved in the embodiments of the present application, the directional indications are only used to explain a certain posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication also changes accordingly.

In addition, if there are descriptions related to "first", "second", etc. in the embodiments of the present application, the descriptions of "first", "second", etc. are only for the purpose of description, and should not be construed as indicating or implying Its relative importance or implicitly indicates the number of technical features indicated. Thus, a feature delimited with "first", "second" may expressly or implicitly include at least one of that feature. In addition, the technical solutions between the various embodiments can be combined with each other, but must be based on the realization by those of ordinary skill in the art. When the combination of technical solutions is contradictory or cannot be realized, it should be considered that the combination of such technical solutions does not exist. , is not within the scope of protection claimed in this application.

In the first embodiment, the present application provides a kit, as shown in Figures 1-3, Figure 1 is a schematic structural diagram of an embodiment of the kit provided by the present application, and Figure 2 is a schematic diagram of the kit shown in Figure 1. Schematic diagram of exploded view; FIG. 3 is a schematic cross-sectional structure diagram of the kit shown in FIG.

Specifically, as shown in FIGS. 1 and 2 , the box body 10 includes a forepool 101 and an installation cavity 102 communicating with the forepool 101 . In this embodiment, the front cell 101 is an electrical impedance detection cell, and two groups are provided, which are respectively used to perform WBC (white blood corpuscle, white blood cell) detection and RBC (red blood cells, red blood cell) detection. In other embodiments, one group or at least three groups, etc. may also be set in the front pool 101 for the detection of red blood cells, white blood cells or other items, which may be set according to actual needs.

As shown in FIG. 3 , the sealing ring 20 is disposed in the installation cavity 102, and is located on the side of the installation cavity 102 close to the fore tank 101. The microporous sheet 30 is provided with micropores 31 that allow cells to pass through one by one. The sealing ring 20 is on the side away from the front tank 101 , and the rear tank body 40 is located on the side of the microporous sheet 30 away from the sealing ring 20 . The rear tank body 40 is snap fit, screw fit, interference fit, laser welding fit or adhesive fit with the installation cavity 102 .

In this embodiment, the sealing ring 20 , the microporous sheet 30 and the rear tank body 40 are sequentially arranged in the installation cavity 102 , and the microporous sheet 30 and the sealing ring 20 are pressed and fixed on the box body 10 through the rear tank body 40 , In this way, the inner end of the mounting cavity 102 is well sealed, the reliability of the kit is improved, and the processing and assembly of the kit are facilitated. Here, the inner end of the installation cavity 102 refers to the end of the installation cavity 102 that is close to the front pool 101 .

As shown in FIG. 3 , the box body 10 includes a through hole 103 that communicates with the front pool 101 and the installation cavity 102 , and the sealing ring 20 is installed at the through hole 103 and fits with the installation cavity 102 , so that when the sealing ring 20 is put in , the inclination will not occur, the installation of the sealing ring 20 is convenient, and the liquid in the front tank 101 will not enter the rear tank body 40 from the edge of the sealing ring 20, which improves the reliability of the detection of the kit.

Further, the ratio of the diameter of the micro-hole sheet 30 to the diameter of the via hole 103 is not less than 1.7, and the diameter of the micro-hole sheet 30 is not larger than the inner diameter of the mounting cavity 102 , for example, the diameter of the micro-hole sheet 30 and the diameter of the via hole 103 If the ratio is 1.7, 1.75 or 1.8, etc., the diameter of the microporous sheet 30 may be equal to the inner diameter of the installation cavity 102 or slightly smaller than the inner diameter of the installation cavity 102 . In this way, it is convenient for the rear tank body 40 to press the microporous sheet 30, preventing liquid from entering the rear tank body 40 from the edge of the microporous sheet 30, and facilitating the installation of the microporous sheet 30 and saving the assembly time of the microporous sheet 30. .

Optionally, the box body 10 , the sealing ring 20 , the microporous sheet 30 and the rear tank body 40 are independent pieces. In a specific embodiment, the microporous sheet 30 and the sealing ring 20 are bonded to the box body 10 , and the rear pool body 40 is bonded to the box body 10 . In another specific embodiment, the microporous sheet 30 and the sealing ring 20 are bonded to the rear pool body 40 , and the rear pool body 40 is bonded to the box body 10 . In this way, the assembly difficulty is reduced.

In other embodiments, the sealing ring 20 , the microporous sheet 30 and the rear pool body 40 can be integrated structural parts to reduce the number of assembly parts, reduce assembly difficulty, and save assembly time.

The box body 10 and/or the rear pool body 40 may be a plastic body. The microporous sheet 30 can be a plastic sheet or a ceramic sheet. The material cost of the plastic sheet or the ceramic sheet is relatively cheap, and can be used as a one-time-use product without using expensive materials that can be repeatedly cleaned and used. The sealing ring 20 can be injection-molded with a relatively soft plastic material by a secondary injection molding process.

Further, as shown in FIG. 2 and FIG. 3 , the box body 10 is provided with a front pool electrode 60 corresponding to the front pool 101 , the back pool body 40 is formed with a drainage cavity 41 (also called a back pool), the front pool 101 and the drainage cavity 41 are formed. The cavities 41 communicate through the micropores 31 . The back cell body 40 is provided with a back cell electrode 50 extending toward the drainage cavity 41 . The outer ends of the front battery electrode 60 and the rear battery electrode 50 (that is, the two ends that are far away from each other) are used to connect the working voltage, and the inner ends of the front battery electrode 60 and the rear battery electrode 50 (that is, the two ends that are close to each other) In liquid contact with the sample to be tested, the liquid level of the sample liquid to be tested in the fore cell 101 will be higher than the fore cell electrode 60, and the drainage cavity 41 will be filled with the sample liquid to be tested during detection.

In the embodiment of the present application, the axis of the front battery electrode 60 and the axis of the rear battery electrode 50 are roughly on a straight line. Experimental verification shows that the detection accuracy is relatively high when the axis of the front battery electrode 60 and the rear battery electrode 50 are coaxial. In other embodiments, the axis of the front battery electrode 60 and the axis of the rear battery electrode 50 may not be on the same straight line.

In the impedance channel, during the calculation of cells, the liquid in the fore cell 101 passes through the microporous sheet 30, and the drainage cavity 41 (back cell) is slowly filled with liquid. One surface or both sides of the microporous sheet 30 may be provided with a concave confinement guide surface 32 around the micropore 31 , and the concave confinement guide surface 32 may be a spherical or conical surface, so that the liquid entering and exiting the micropore 31 can be adjusted. diversion.

Further, the front cell electrode 60 and/or the rear cell electrode 50 may be cylindrical electrodes. However, in this way, the liquid column passing through the micropores 31 of the microporous sheet 30 may hit the rear cell electrode 50, and then cause backflow to impact the microporous sheet 30. This phenomenon, on the one hand, affects the stability of the signal, and on the other hand On the one hand, M waves may be generated, reducing the reliability of detection.

In order to improve the above-mentioned problems, in some embodiments, the inner end face of the back cell electrode 50 can be set as a convex surface. In this way, the liquid recoil flow can be uniformly dispersed from the center to the surrounding, so that the recoil flow does not directly flow to the microporous sheet 30, so as to reduce the phenomenon of cell particle recoil. The inner end face refers to the front cell 101 as a reference, the end of the rear cell electrode 50 pointing to the fore cell 101 is the inner end face, and the end away from the fore cell 101 is the outer end face.

Further, the rear tank body 40 is provided with a liquid outlet 44 , the liquid outlet 44 communicates with the installation cavity 102 , and the liquid outlet 44 is used to discharge the gas or liquid in the drainage cavity 41 . For the structure of the rear pool body 40, reference may also be made to the introduction in the following second embodiment, specifically the introduction in the embodiments shown in FIG. 4 and FIG. 5 , which will not be repeated here.

The distance between the inner end surface of the rear cell electrode 50 and the microporous sheet 30 is not less than 5 mm. By limiting the distance relationship between the microporous sheet 30 and the inner end face of the back cell electrode 50, the microporous sheet 30 is prevented from being too close to the back cell electrode 50, so as to give the liquid a sufficient buffer distance, and it is convenient for the negative pressure to timely remove the liquid in the drainage cavity 41 from the back cell electrode 50. The liquid outlet 44 leads out.

Further, the inner end of the installation cavity 102 is communicated with the front pool 101 through the via hole 103 , and the outer end of the installation cavity 102 is an open end for receiving the rear pool body 40 to be loaded. The end face of the installation cavity 102 can be provided with a positioning part (not shown), and the rear pool body 40 can be provided with a matching part (not shown), and the matching part is connected with the positioning part to perform positioning and installation of the rear pool body 40 .

In a specific embodiment, a positioning protrusion may be provided on the outer end surface of the installation cavity 102 as a positioning portion, and a positioning groove matched with the positioning protrusion may be provided on the rear pool body 40 as a matching portion. When installing the rear pool body 40 , the positioning protrusions cooperate with the positioning grooves to position the rear pool body 40 . In this way, the assembly difficulty of the rear tank body 40 can be reduced and assembly time can be saved.

In other embodiments, a positioning groove may be provided on the outer end surface of the installation cavity 102 as a positioning portion, and a positioning protrusion adapted to the positioning groove may be provided on the rear pool body 40 as a matching portion. When the body 40 is installed, the positioning protrusions cooperate with the positioning grooves to position and install the rear pool body 40 . In this way, the assembly difficulty of the rear pool body 40 can also be reduced and assembly time can be saved.

To sum up, the kit of this embodiment has a simple structure, a novel fixing method of the microporous sheet 30 , easy processing, low assembly difficulty, and high reliability.

In the second embodiment, the present application also provides a kit, as shown in FIGS. 1-5 , FIG. 4 is a schematic structural diagram of an embodiment of the rear tank body in the kit shown in FIG. 1 , and FIG. 5 is a 1 is a schematic structural diagram of another embodiment of the rear cell body in the kit. The kit of this embodiment includes an electrical impedance detection cell (not marked in the figure), which is used for electrical impedance detection of the sample to be detected. The electrical impedance The detection cell includes a back cell body 40 and a back cell electrode 50. The back cell electrode 50 is embedded on the back cell body 40. The back cell electrode 50 and the back cell body 40 can be integrally injection molded or detachably connected.

The back pool body 40 is formed with a drainage cavity 41, the back pool electrode 50 is arranged on the back pool body 40 and extends to the drainage cavity 41, and the inner end face of the back pool electrode 50 is a convex surface. Specifically, the inner end of the back pool electrode 50 can be provided with It is hemispherical, and the inner end face of the rear cell electrode 50 is the end face of one side of the rear cell electrode 50 extending toward the drainage cavity 41 . For the convex structure of the back cell electrode 50, please refer to the introduction of the first embodiment, which will not be repeated here.

Further, as shown in FIG. 4 , the back cell body 40 includes a bottom wall 43 and a side wall 42 , the bottom wall 43 is connected to the side wall 42 to form a drainage cavity 41 , and the back cell electrode 50 is disposed on the bottom wall 43 . A liquid outlet 44 is provided on the side wall 42 , and the liquid outlet 44 communicates with the drainage cavity 41 for discharging the gas or liquid in the drainage cavity 41 . In the embodiment shown in FIG. 4 , the bottom wall 43 is a plane, and the plane where the bottom wall 43 is located is inclined relative to the side wall 42 , and the liquid outlet 44 is provided at the intersection of the bottom wall 43 and the side wall 42 , and is located at the intersection of the bottom wall 43 and the side wall 42 . The side away from the microporous sheet 30 . In this embodiment, the bottom wall 43 is set as an upwardly inclined plane, and serves as a drainage surface, so that the liquid flows through the microporous sheet 30 to the rear cell electrode 50 and then is led to the liquid outlet 44 by the bottom wall 43, so that the liquid can be drained from the outlet 44 easily. The liquid flows out from the liquid port 44 to avoid liquid accumulation, reduce the signal disturbance caused by the particle gyration near the back cell electrode 50, and improve the reliability of detection.

In addition, the liquid outlet 44 is located at the intersection of the side wall 42 and the bottom wall 43. In this way, the end of the slope formed by the bottom wall 43 is close to the liquid outlet 44. When the liquid in the drainage cavity 41 is drained through the bottom wall 43, it is The liquid outlet 44 will flow straight out, further reducing the retention of the liquid.

In another embodiment, as shown in FIG. 5 , in this embodiment, the bottom wall 43 also serves as a drainage surface to drain the liquid in the drainage cavity 41 . Specifically, the distance from the bottom wall 43 to the microporous sheet 30 gradually decreases in the direction of the edge of the bottom wall 43 from the position where the bottom wall 43 is provided with the rear cell electrode 50 . That is, the shape of the bottom wall 43 may be a bell mouth shape, so as to drain the liquid in the drainage cavity 41 .

Further, as shown in FIG. 5, the liquid outlet 44 is arranged at the intersection of the bottom wall 43 and the side wall 42, that is, the liquid outlet 44 can be arranged close to the bottom wall 43, so that when the liquid in the back pool passes through the bottom wall 43 When the drainage is finished, it will flow straight out of the liquid outlet 44 to reduce the stay of the liquid.

Further, the maximum distance value of the distance between the inner end surface of the rear cell electrode 50 and the bottom wall 43 is less than 0.5 mm. The distance by which the inner end surface of the rear cell electrode 50 protrudes from the inner surface of the bottom wall 43 is small, which reduces the phenomenon of liquid jamming of the rear cell electrode 50 .

Further, the distance between the inner end face of the back cell electrode 50 and the microporous sheet 30 is not less than 5mm, to avoid the microporous sheet 30 from being too close to the back cell electrode 50, so as to give the liquid a sufficient buffer distance to facilitate the drainage of the negative pressure in time. The liquid in the cavity 41 is led out from the liquid outlet 44 .

Optionally, the rear cell electrode 50 is arranged at the center of the bottom wall 43, and the rear cell electrode 50 is coaxially arranged with the drainage cavity 41. In this way, the drafting operation after the mold is formed is convenient.

In the above-mentioned embodiment, the structure of the electrical impedance detection cell is novel, the inner end face of the rear cell electrode 50 is set to be convex, and the bottom wall 43 of the rear cell body 40 forms a drainage surface to guide the liquid to the liquid outlet 44. This structure. The improvement is convenient for the liquid to flow out from the liquid outlet hole, and can reduce the influence of signal disturbance caused by particle recoil and gyration, thereby improving the accuracy of sample detection and the accuracy of detection results.

In the third embodiment, the application also provides a kit, please refer to FIG. 6 and FIG. 7 , FIG. 6 is a schematic structural diagram of another embodiment of the kit provided by the application, and FIG. 7 is shown in FIG. 6 An exploded schematic diagram of the kit, the kit includes a box body 10, the box body 10 includes a first box body 11 and a second box body 12, and the first box body 11 and the second box body 12 are detachably connected.

The first box body 11 is provided with a first detection cell position, which is used for electrical impedance detection, and the second box body 12 is provided with a second detection cell position, which is used for optical detection. In this embodiment, the first detection pool can be used for WBC detection and RBC detection, and the second detection pool can be used for specific protein detection. In other embodiments, the second detection pool can also be used in conjunction with other biochemical detections , immunoassays, etc.

In this embodiment, the first box body 11 and the second box body 12 are detachably connected. In this way, the storage and transportation of the box body 10 are facilitated. In other embodiments, the first box body 11 and the second box body 12 may also be integrally formed to improve the reliability of the connection between the first box body 11 and the second box body 12 .

In the embodiment shown in FIG. 6 and FIG. 7 , at least one card slot 111 is provided on one side of the first box body 11 , and a protrusion 121 matched with the card slot 111 is arranged on one side of the second box body 12 . The slot 111 cooperates with the protrusion 121 to hang the second box body 12 on the first box body 11 . In this way, the connection structure of the first box body 11 and the second box body 12 is simple, and the assembly and disassembly processes are simple. Preferably, the number of the card slots 111 and the bumps 121 can be set to a plurality, so as to make the connection between the second box body 12 and the first box body 11 more firm.

Further, the side of the card slot 111 away from the first box body 11 is set as a constriction, that is, the opening area of the side of the card slot 111 away from the first box body 11 is smaller than that of the side of the card slot 111 close to the first box body 11 . area of the bottom wall. In this way, the second box body 12 can be effectively prevented from falling off from the first box body 11 .

Further, the cross-sectional shape of the card slot 111 along the first plane may be a trapezoid, wherein the first plane is a plane perpendicular to the thickness direction of the first box body 11 . In this way, the connecting parts of the first box body 11 and the second box body 12 have a regular shape, which facilitates the processing of the box body 10 .

In another embodiment, as shown in FIGS. 8 and 9 , FIG. 8 is a schematic structural diagram of another embodiment of the kit provided by the present application, and FIG. 9 is an exploded schematic diagram of the kit shown in FIG. 8 , specifically One side of the first box body 11 is provided with a hanger 112 , and the edge of the second box body 12 is suspended on the hanger 112 .

Specifically, as shown in FIG. 9 , a hanging hole 1120 is formed around the hanger 112 , the second box body 12 is inserted in the hanging hole 1120 , and the outer edge of the second box body 12 is supported on the hanger 112 . In this way, the force of the second box body 12 can be more uniform, and the second box body 12 can be more stably fixed on the hanger 112 .

Further, the shape of the cross-section of the hanging hole 1120 may be a rectangle, a circle, a trapezoid, a triangle, or a special shape. The shape of the hanging hole 1120 may be adapted to the shape of the second box body 12 . For example, when the second box body 12 is rectangular, the shape of the hanging hole 1120 is also set to be rectangular.

In other embodiments, the edges of at least two ends of the second box body 12 are suspended on the hangers 112 to support the second box body 12 by the hangers 112 . In this way, the structure of the box body 10 can be simplified and the production is convenient. For example, the hanger 112 may directly include two or three support rods, which are used to support the edges of two or three ends of the second box body 12, so that the material cost can also be saved.

Further, as shown in FIG. 7 and FIG. 8 , the first detection cell position may include a fore cell 101 , and two groups of the fore cell 101 may be set, which are respectively used to perform white blood cell detection and red blood cell detection. The first detection pool position may further include at least one pipette tip placement pool 113 , diluent pool 114 , hemolytic agent pool 115 and sample accommodating pool 116 , wherein the pipette tip placement pool is used for accommodating the pipette tip 60 and the diluent pool 114 For encapsulating the diluent, the hemolyzing agent tank 115 is used for encapsulating the hemolyzing agent; the first detection tank position may further include a sample dilution tank 117, and the sample dilution tank 117 is used for sample dilution. The plurality of pool positions are arranged in a straight line. In this way, it is convenient for the pipetting device to move in a short path during automatic detection, wherein the pipetting device is used to transfer and mix the liquid in each pool body.

In order to ensure the light transmittance of the material, the first box body 11 can be made of a transparent PP (Polypropylene, polypropylene) material. In other embodiments, the front pool 101 may also be provided with a light-transmitting detection window (not shown in the figure) for performing optical detection. The light transmittance and smoothness of the light-transmitting detection window may be the same as or higher than those of the front pool 101 . other parts of the pool 101.

Further, the second detection cell includes a plurality of placement holes 123, and the placement holes 123 are used to place an optical detection cup assembly (not shown) for optical detection. The placement hole 123 may include a first placement hole 121 and a second placement hole 122 that are spaced apart. The optical detection cup assembly includes an optical measurement cup (not shown in the figure) and a reagent cup (not shown in the figure). The optical measurement cup can be used for the detection of specific proteins. The material of the optical measurement cup can be transparent PC (Polycarbonate, polycarbonate) material. Reagent cups are used to store reagents. The first placement hole 121 may be used to place the optical measurement cup, and the second placement hole 122 may be used to place the detection cup.

The shapes of the first placement hole 121 and the second placement hole 122 are different. For example, the shape of the first placement hole 121 can be set as a circle, and the shape of the second placement hole 122 can be set as a rectangle for differentiation.

Optionally, the second detection pool position can be used to place two or more optical detection cup assemblies, that is, the number of the first placement hole 121 and the second placement hole 122 can be both two or more. Used for detection of different projects.

The box body 10 of the reagent kit in the above-mentioned embodiment can be detachably connected, and the assembly and disassembly process is simple, which is convenient for transportation.

In the fourth embodiment, the present application also provides a kit. Please refer to FIG. 10 . FIG. 10 is a schematic structural diagram of another embodiment of the kit provided by the present application. The kit of this embodiment includes a box body 10 and a At least two pipette tips 60 . Wherein, the box body 10 is provided with at least two pipette tip placement pools for placing the above at least two pipette tips 60 respectively. Among them, the pipette head 60 is used to be assembled on the pipette to cooperate with the pipetting operation.

Each pipette head 60 has a certain volume, and the sample liquid or reagent liquid will remain in the pipette head 60 during the pipetting operation. The volumes of at least two pipette heads 60 in this embodiment are different. For example, the reagent kit may be configured with two pipette heads 60, and the volumes of the two pipette heads 60 are different. In actual use, different sample solutions or reagent solutions can use pipette tips 60 with different volumes. For example, when pipetting 10 μL is required, in order to ensure the pipetting accuracy, a pipette tip 60 with a capacity of 10 μL to 20 μL can be selected for pipetting. . In this way, the sample adding accuracy can be improved, thereby improving the accuracy of the detection result of the sample.

Further, as shown in FIG. 10 , the pipette head 60 includes a pipe body 61 and a fixing part 62 , the fixing part 62 is fixed on the periphery of the pipe body 61 , and the pipe body 61 is formed with a liquid suction port 63 for liquid suction/spitting , the fixing part 62 is located on the side of the pipe body 61 away from the liquid suction port 63 , and the fixing part 62 is used to fix the pipe body 61 in the pipette tip placing pool 113 . The shapes of the fixing parts 62 of the pipette heads 60 with different volumes are different, so that the pipette heads 60 can be distinguished by the shapes of the fixing parts 62 to prevent wrong selection of the pipette heads 60 . In other embodiments, the shape of the fixing portion 62 of the pipette tips 60 with different volumes may also be the same, so as to facilitate the production of the pipette tips 60 .

The inner diameter of the pipette tip placement pool 113 on the box body 10 may be the same or different. For example, in order to facilitate differentiation, at least two pipette tip placement pools 113 have different inner diameters for placing different pipette tips 60 respectively. Specifically, the pipette tip 60 with a large volume can be inserted into the pipette tip placement pool 113 with a larger inner diameter, and the pipette tip 60 with a small volume can be inserted into the pipette tip placement pool 113 with a smaller inner diameter.

In order to enable the pipette tips 60 to adapt to the pipette tip placement pools 113 with different inner diameters, the fixing portion 62 of the pipette tips 60 may be arranged in a stepped shape. Specifically, the fixed portion 62 of the pipette head 60 includes at least two steps, the at least two steps are parallel and spaced apart toward the side away from the liquid suction port 63 , and the outer diameters of the at least two steps are toward the side away from the liquid suction port 63 . direction gradually increases. By arranging the fixing portion 62 into steps of different sizes, the pipette tip 60 can be adapted to the pipette tip placement pool 113 with different inner diameters. In this way, the insertion of the pipette tip 60 can be free from the restriction of the inner diameter of the pipette tip placement pool 113 . , so that the pick-and-place of the pipette head 60 is convenient.

When the pipette tip 60 is placed in the pipette tip placing pool 113 , the pipe body 61 is inserted into the pipette tip placing pool 113 , and the pipette tip 60 can be supported on the surface of the box body 10 by the fixing portion 62 .

Further, a sinking table 1131 may be provided at the opening of the pipette head placing pool 113. When the pipette head 60 is placed in the pipette head placing pool 113, the fixing portion 62 may be supported on the sinking table 1131, so that the suction pipe head can be connected to the pipette head through the sinking table 1131. 60 for support.

Optionally, the lengths and/or calibers of the pipette tips 60 with different volumes are also different, so as to facilitate the different pipette tips 60 to be distinguished. For example, the length/diameter of the pipette tips 60 with smaller volumes is also smaller, so as to facilitate distinguishing different pipette tips 60 .

Further, a hydrophobic coating may be provided on the inner wall of the pipette head 60 to prevent the pipette head 60 from hanging with liquid and improve the sample adding accuracy of the pipette head 60 .

Further, the pipette head 60 may be provided with a mark, and the mark may be an electronic label, a two-dimensional code or a barcode, etc., to record the relevant parameters of the pipette head 60 .

The kit of the above embodiment can improve the accuracy of sample addition, thereby improving the accuracy of the sample detection result.

In the fifth embodiment, the present application also provides a POCT (point-of-care testing, point-of-care testing) blood cell analyzer. Please refer to FIG. 11 . A schematic diagram of the structure of an embodiment, the POCT blood cell analyzer includes a pipette 70, and the end of the pipette 70 is used to cooperate with the pipette head 60 of the above embodiment to perform pipetting operations.

Specifically, the end of the pipette 70 is provided with a suction head 71, and the suction head 71 is used for socketing the pipette head 60. The pipette head 60 has a certain volume, and the sample liquid or reagent liquid will remain in the pipette head during the pipetting operation 60 without entering the inside of the pipette 70. When the pipetting, moving, and spitting operations are completed and the sample liquid or reagent liquid needs to be replaced, the used pipette tip 60 can be discarded and the unused pipette can be re-installed The head 60, so that the new sample liquid or reagent liquid can be pipetted, so the pipette 70 will not be polluted, so there is no need to clean the pipette 30 after each use, eliminating the need for complicated Cleaning components and cleaning processes improve detection efficiency.

Further, the pipette tips 71 at the end of the pipette 70 are arranged in a stepped shape, so as to be adapted to the pipette tips 60 of different diameters. In this way, one pipette head 71 can be used to connect pipette heads 60 with different diameters, so as to simplify the pipetting process and save material costs.

The embodiments of the present application also provide a POCT (point-of-care testing, point-of-care testing) blood cell analyzer, which includes the kit of any of the foregoing embodiments and a detection seat matched with the kit, and the POCT blood cell analyzer is used for Blood sample analysis. For the specific structure of the kit, please refer to the accompanying drawings and related text descriptions of the foregoing embodiments, which will not be repeated here.

The above are only the embodiments of the present application, and are not intended to limit the scope of the patent of the present application. Any equivalent structure or equivalent process transformation made by using the contents of the description and drawings of the present application, or directly or indirectly applied in other related technical fields, All are similarly included in the scope of patent protection of the present application.

Claims (10)

1. A kit, comprising:

the box body comprises a forebay and an installation cavity communicated with the forebay;

the sealing ring is arranged in the mounting cavity and is positioned on one side of the mounting cavity close to the forebay,

a microporous sheet provided with micropores allowing cells to pass through one by one, the microporous sheet being disposed in the mounting cavity and located on a side of the sealing ring away from the front tank,

the rear tank body is abutted against one side, away from the sealing ring, of the microporous sheet and used for fixing the microporous sheet and the sealing ring on the box body, a drainage cavity is formed in the rear tank body, and the front tank and the drainage cavity are communicated through the micropores.

2. The reagent cartridge according to claim 1, wherein the cartridge body includes a through hole communicating the front pool and the installation cavity,

the ratio of the diameter of the microporous sheet to the diameter of the through hole is not less than 1.7, and the diameter of the microporous sheet is not greater than the inner diameter of the installation cavity.

3. The kit of claim 2, wherein the sealing ring is a clearance fit with the mounting cavity.

4. The kit according to claim 1, wherein the rear cell body is bonded to a case body, and the microporous sheet and the gasket are bonded to the case body; or,

the rear tank body is bonded with the box body, and the microporous sheet and the sealing ring are bonded on the rear tank body.

5. The kit according to claim 1, wherein a positioning protrusion is arranged on the outer end face of the mounting cavity, a positioning groove is arranged on the rear tank body, and the positioning protrusion is matched with the positioning groove to position the rear tank body; or,

the outer end face of the mounting cavity is provided with a positioning groove, a positioning bulge is arranged on the rear pool body, and the positioning bulge is matched with the positioning groove to position the rear pool body.

6. The kit according to claim 1, wherein the front cell is provided with a front cell electrode, the rear cell body is provided with a rear cell electrode extending to the drainage chamber, the front cell electrode and the rear cell electrode are respectively positioned at two sides of the microporous sheet at intervals, and the inner end surface of the rear cell electrode is convex.

7. The kit of claim 1, wherein the rear cell body comprises a bottom wall and a side wall, the bottom wall connecting the side walls to form the drainage lumen, the rear cell electrode disposed on the bottom wall,

the side wall is provided with a liquid outlet which is communicated with the drainage cavity, the bottom wall is a plane, the plane and the side wall are obliquely arranged, and the liquid outlet is arranged at the intersection of the bottom wall and the side wall and is positioned at one side far away from the microporous sheet.

8. The kit of claim 1, wherein the rear cell body comprises a bottom wall and a side wall, the bottom wall connecting the side walls to form the drainage lumen, the rear cell electrode disposed on the bottom wall,

the distance from the bottom wall to the microporous sheet is gradually reduced in a direction from a position where the bottom wall is provided with the rear cell electrode to an edge of the bottom wall.

9. The kit of claim 1, wherein one or both surfaces of the microporous sheet have a flow guide surface with a concave limit at the periphery of the micropores.

10. A POCT blood cell analyzer, comprising the kit according to any one of claims 1 to 9 and a test seat cooperating with the kit, wherein the POCT blood cell analyzer is used for analyzing and testing a blood sample.

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