CN114832655A - Raw material dissolving device for preparing serum-free cell culture medium - Google Patents
Raw material dissolving device for preparing serum-free cell culture medium Download PDFInfo
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- CN114832655A CN114832655A CN202210515529.1A CN202210515529A CN114832655A CN 114832655 A CN114832655 A CN 114832655A CN 202210515529 A CN202210515529 A CN 202210515529A CN 114832655 A CN114832655 A CN 114832655A
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- 210000002966 serum Anatomy 0.000 claims abstract description 44
- 238000009825 accumulation Methods 0.000 claims abstract description 15
- 238000005192 partition Methods 0.000 claims abstract description 5
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 239000002245 particle Substances 0.000 claims description 21
- 238000000926 separation method Methods 0.000 claims description 17
- 241000521257 Hydrops Species 0.000 claims description 9
- 206010030113 Oedema Diseases 0.000 claims description 9
- 238000007599 discharging Methods 0.000 claims description 6
- 238000005096 rolling process Methods 0.000 claims description 6
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- 239000000463 material Substances 0.000 description 1
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Abstract
The invention discloses a raw material dissolving device for preparing a serum-free cell culture medium, which relates to the technical field of cell culture and has the technical scheme that: the device comprises a base, wherein a liquid supply device and a dissolving device are fixedly arranged at the top of the base; the liquid supply device is connected with the top of the dissolving device; the dissolving device comprises a fixed box body, a vibrating box body, a plurality of first supports, a cylinder body, a liquid inlet device, a liquid discharge device, a vibration device and a liquid accumulation device; the bottom of the fixed box body is fixedly connected with the first supports, and the bottoms of the first supports are fixedly connected with the top of the base; the vibrating box body is located the fixed box body, the outer side wall of the vibrating box body is connected with the inner side wall of the fixed box body in a sliding mode, and a partition board which divides the inner space of the vibrating box body into a plurality of dissolving chambers is fixedly arranged in the vibrating box body. The dissolving device not only can prepare serum-free cell culture media, but also can prepare cell culture media with different serum concentrations required by a cell adaptation stage.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a raw material dissolving device for preparing a serum-free cell culture medium.
Background
Stem cell transplantation therapy is a focus of recent clinical frontier research; stem cell transplantation achieves the goal of healing by transplanting healthy stem cells into a patient to repair or replace damaged cells or tissues. Currently, stem cell transplantation has been reported for the treatment of internal and surgical diseases including nervous system diseases, immune system diseases, skeletal diseases, and the like. Therefore, the standardized culture of stem cells is important for the clinical therapeutic effect of stem cells.
Serum-free culture technology is a milestone in stem cell culture research. The serum-free culture medium is composed of known components, so that a powerful tool is provided for researching and highlighting the fundamental problem of cell growth, proliferation and differentiation mechanism in life science, and a foundation is laid for the application of modern biotechnology such as genetic engineering, protein engineering, cell engineering, monoclonal antibodies and the like.
The serum-free culture medium is a synthetic culture medium which can maintain cells to grow and propagate in vitro for a longer time without adding serum; serum-free media and reagents are widely used to culture mammalian cells, including mesenchymal stem cells, to prepare standardized clinical-grade stem cell banks. The basic components of the serum-free culture medium are a basic culture medium and an additive component. The basic culture medium used by the existing serum-free culture medium is prepared by dissolving a powder raw material in ultrapure water.
Serum is the most basic additive in animal cell culture, and particularly in primary culture or in the case of poor cell growth, a culture solution containing serum is often used for culture, and after the cells grow vigorously, the culture solution is replaced by a serum-free culture solution. The cells are transferred into serum-free medium for culture, and an adaptation process is required, and the serum concentration is generally reduced gradually from 10% to 5%, 3% and 1% until the cells are cultured in the serum-free medium.
When the culture medium in the cell adaptation stage is prepared at present, different amounts of serum are added into the culture medium after the preparation is performed by a dissolving device, so that the operation is inconvenient; there is a need for a lysing device that can simultaneously produce different serum concentrations.
Disclosure of Invention
The purpose of the present invention is to provide a raw material dissolver for serum-free cell culture medium preparation, which can prepare not only a serum-free cell culture medium but also a cell culture medium of different serum concentrations required for the cell adaptation stage.
The technical purpose of the invention is realized by the following technical scheme: the raw material dissolving device for preparing the serum-free cell culture medium comprises a base, wherein a liquid supply device and a dissolving device are fixedly arranged at the top of the base; the liquid supply device is connected with the top of the dissolving device;
the dissolving device comprises a fixed box body, a vibrating box body, a plurality of first supports, a cylinder body, a liquid inlet device, a liquid discharge device, a vibration device and a liquid accumulation device; the bottom of the fixed box body is fixedly connected with the first supports, and the bottoms of the first supports are fixedly connected with the top of the base; the vibrating box body is positioned in the fixed box body, the outer side wall of the vibrating box body is connected with the inner side wall of the fixed box body in a sliding mode, and a partition plate for dividing the inner space of the vibrating box body into a plurality of dissolving chambers is fixedly arranged in the vibrating box body; the top of the cylinder is positioned right above the vibration box body and is connected with the liquid inlet device, and the bottom of the cylinder penetrates through the vibration box body and the bottom of the fixed box body and is connected with the vibration device; the cylinder body is fixedly connected with the vibration box body, and the cylinder body is connected with the fixed box body in a sliding manner; the liquid inlet device is positioned in the cylinder; the liquid inlet end of the liquid discharge device is communicated with the bottoms of the plurality of dissolving chambers, and the liquid outlet end of the liquid discharge device penetrates through the bottom of the fixed box body to be connected with the liquid accumulating device; the effusion device and the oscillation device are both connected with the base;
the liquid inlet device comprises a movable liquid inlet pipe, a Venturi liquid separating head I, a plurality of liquid outlet branch pipes and a plurality of electromagnetic valves I; the top of the first Venturi liquid separation head is fixedly connected with the bottom of the movable liquid inlet pipe; the movable liquid inlet pipe is connected with the liquid supply device in a sliding way; the liquid outlet branch pipes are simultaneously connected with the liquid outlet end at the bottom of the Venturi liquid separating head; the end parts of the liquid outlet branch pipes, which are far away from the Venturi liquid separating head I, penetrate through the side wall of the cylinder body, and the liquid outlet ends of the liquid outlet branch pipes are communicated with the dissolving chambers; the first electromagnetic valves are fixedly arranged on the liquid outlet branch pipes respectively;
the liquid drainage device comprises a plurality of liquid drainage branch pipes, a plurality of electromagnetic valves II, a Venturi liquid separation head II and a liquid drainage main pipe; the tops of the liquid discharge branch pipes are simultaneously communicated with the bottom of the dissolving chamber, and the bottoms of the liquid discharge branch pipes penetrate through the bottom of the fixed box body and are simultaneously connected with the top of the Venturi liquid separation head II; the bottom of the Venturi liquid separation head II is fixedly connected with the top of the liquid drainage main pipe, and the liquid drainage main pipe is movably connected with the liquid accumulation device; the second electromagnetic valves are respectively arranged on the liquid discharge branch pipes;
the oscillating device comprises a limiting plate, a first spring, a second bracket, a roller, a rotating motor, a first rotating shaft, a limiting block and a cam; the top of the limiting plate is fixedly connected with the bottom of the barrel; the spring is sleeved on the barrel, the top of the first spring is fixedly connected with the bottom of the fixed box body, and the bottom of the first spring is fixedly connected with the top of the limiting plate; the top of the second support is fixedly connected with the bottom of the limiting plate, the roller is sleeved on the second support, and the rolling surface of the roller is in contact with the rolling surface of the cam; the cam is sleeved on the first rotating shaft, the end part of the first rotating shaft is fixedly connected with the output end of the rotating motor, the bottoms of the rotating motor and the limiting block are fixedly connected with the top of the base, the limiting block is sleeved on the first rotating shaft, and the limiting block is movably connected with the first rotating shaft.
By adopting the technical scheme, the oscillation device realizes the vertical reciprocating motion of the whole vibration box body in a mode of contacting the cam with the roller, so that the powder raw material, the serum and the pure water are fully mixed and dissolved; the spring is sleeved at the position of the barrel outside the fixed box body, and the two ends of the spring are simultaneously connected with the limiting plate and the fixed box body, so that the roller can be in close contact with the cam, and stable up-and-down reciprocating motion is realized; the liquid outlet ends of the liquid outlet branch pipes are respectively arranged in different dissolving chambers, and each liquid outlet branch pipe is provided with a second electromagnetic valve, so that each dissolving chamber contains different amounts of serum, and cell culture media with different serum concentrations are prepared by dissolving.
The invention is further configured to: the liquid supply device comprises two liquid supply pipes, a particle funnel, a pump body, a serum storage device, a first flow control valve and a pure water storage tank; the liquid inlet and outlet ends of the pump body are respectively connected with the two liquid supply pipes; the end part of a liquid supply pipe connected with a liquid outlet of the pump body is fixedly connected with the top of the fixed box body, the movable liquid inlet pipe is positioned in the liquid supply pipe, and the liquid supply pipe is in sliding connection with the movable liquid inlet pipe; the serum storage device and the particle funnel are respectively connected with the side walls of the two liquid supply pipes; the end part of a liquid supply pipe connected with the liquid inlet end of the pump body is connected with a pure water storage tank; the first flow control valve is arranged on a liquid supply pipe connected with the pure water storage tank, and the first flow control valve is positioned between the serum storage device and the pure water storage tank; a shielding device is arranged in the particle funnel.
Through adopting above-mentioned technical scheme, link to each other the pump body with two feed pipes to be connected serum storage device, granule funnel and pure water bin with the feed pipe simultaneously, thereby can go into the vibrating box with powder raw materials, pure water and serum simultaneously.
The invention is further configured to: the shielding device comprises a stepping motor, a bearing, a second rotating shaft and two baffles; the stepping motor and the bearing are fixedly connected with the outer side wall of the particle funnel, the second rotating shaft and the two baffles are positioned in the particle funnel, and two ends of the second rotating shaft are respectively fixedly connected with the output end of the stepping motor and the bearing; two the baffle is semi-circular, and two the straight line limit of baffle is simultaneously with two pivot fixed connection, two the pitch arc edge of baffle contacts with particle funnel's inside wall.
Through adopting above-mentioned technical scheme, baffle and the vertical section internal diameter of particle funnel contact to archives can rotate along with the rotation of second pivot, can control the opening size like this according to the rotation angle of baffle, thereby the speed that powder raw materials spills downwards in the control particle funnel, when preparing serum-free cell culture medium or the cell culture medium that has a small amount of serum like this, can fix the quantity of powder raw materials, the batch production of being convenient for.
The invention is further configured to: the serum storage device comprises a serum storage box, a liquid outlet pipe and a flow control valve II; the bottom and the drain pipe of serum bin are connected, flow control valve two is installed on the drain pipe, drain pipe and feed pipe intercommunication.
By adopting the technical scheme, the second flow control valve is arranged on the liquid outlet pipe, and the serum dosage can be adjusted through the second flow control valve, so that the culture medium for the cell adaptation stage with different serum concentrations can be prepared.
The invention is further configured to: the liquid accumulation device comprises a liquid accumulation box body, a fixed pipe and two fixed devices; the bottom of the fixed pipe is fixedly connected with the top of the effusion box body, the bottom of the liquid drainage main pipe is positioned in the fixed pipe, and the liquid drainage main pipe is connected with the fixed pipe in a sliding manner; two fixing device slidable mounting is in hydrops box both sides, and two fixing device distributes along the axis position mirror symmetry of hydrops box.
Through adopting above-mentioned technical scheme, two fixing device of mirror symmetry installation in hydrops box no matter be to the collection device of flat bottom or the collection device of round bottom, all can put into in the hydrops box and collect the cell culture medium, improved application scope.
The invention is further configured to: the fixing device comprises a handle, a second spring, at least two sliding rods, an arc-shaped fixing plate and an arc-shaped rubber pad; the arc-shaped rubber pad is attached to the arc-shaped fixed plate, the side wall, away from the arc-shaped rubber pad, of the arc-shaped fixed plate is fixedly connected with the second spring and the slide rod, the end portion, away from the side wall of the arc-shaped fixed plate, of the second spring is fixedly connected with the inner side wall of the liquid accumulation box body, and the end portion, away from the arc-shaped fixed plate, of the slide rod is fixedly connected with the handle.
Through adopting above-mentioned technical scheme, arc fixed plate and two fixed connection of spring for under the spring action of spring two, the collection device is tightly held to the arc rubber pad of pasting on arc fixed plate, has improved collection device's application scope.
In conclusion, the invention has the following beneficial effects:
1. the oscillation device realizes the up-and-down reciprocating motion of the whole vibration box body by adopting a mode of contacting a cam with a roller, thereby realizing the full mixing and dissolution of the powder raw material, the serum and the pure water;
2. the spring is sleeved at the position of the barrel outside the fixed box body, and the two ends of the spring are simultaneously connected with the limiting plate and the fixed box body, so that the roller can be in close contact with the cam, and stable up-and-down reciprocating motion is realized;
3. the liquid outlet ends of the liquid outlet branch pipes are respectively arranged in different dissolving chambers, and each liquid outlet branch pipe is provided with a second electromagnetic valve, so that each dissolving chamber contains different amounts of serum, and cell culture media with different serum concentrations are prepared by dissolving.
Drawings
FIG. 1 is a schematic configuration diagram of a source material dissolver for serum-free cell culture medium preparation according to an embodiment of the present invention;
FIG. 2 is an enlarged view at A in FIG. 1;
FIG. 3 is an enlarged view at B in FIG. 1;
FIG. 4 is a schematic view of a shielding device according to an embodiment of the present invention;
FIG. 5 is a front cross-sectional view of a liquid loading apparatus in an embodiment of the invention.
In the figure: 1. vibrating the box body; 2. a second electromagnetic valve; 3. fixing the box body; 4. a second Venturi liquid separation head; 5. a liquid drainage main pipe; 6. a fixed tube; 7. a first bracket; 8. a base; 9. a liquid accumulation box body; 10. a cam; 11. a limiting block; 12. a first rotating shaft; 13. a rotating electric machine; 14. a pump body; 15. a second flow control valve; 16. a pure water storage tank; 17. a first flow control valve; 18. a liquid outlet pipe; 19. a serum storage tank; 20. a roller; 21. a second bracket; 22. a limiting plate; 23. a first spring; 24. a barrel; 25. a partition plate; 26. a dissolution chamber; 27. a particle hopper; 28. a liquid supply tube; 29. a liquid outlet branch pipe; 30. a liquid discharge branch pipe; 31. a baffle plate; 32. a second rotating shaft; 33. a first electromagnetic valve; 34. a first Venturi liquid separation head; 35. a movable liquid inlet pipe; 36. a stepping motor; 37. a bearing; 38. a beaker; 39. a second spring; 40. a grip; 41. a slide bar; 42. an arc-shaped rubber pad; 43. an arc-shaped fixing plate.
Detailed Description
The present invention is described in further detail below with reference to figures 1-5.
Example (b): the raw material dissolving device for preparing the serum-free cell culture medium comprises a base 8, wherein a liquid supply device and a dissolving device are fixedly arranged at the top of the base 8, as shown in fig. 1 to 5; the liquid supply device is connected with the top of the dissolving device;
as shown in fig. 1, the dissolving device comprises a fixed box body 3, a vibrating box body 1, four first supports 7, a cylinder 24, a liquid inlet device, a liquid discharge device, a vibration device and a liquid accumulation device; the bottom of the fixed box body 3 is fixedly connected with the plurality of first supports 7 at the same time, and the bottoms of the four first supports 7 are fixedly connected with the top of the base 8 at the same time; the vibrating box body 1 is positioned in the fixed box body 3, the outer side wall of the vibrating box body 1 is connected with the inner side wall of the fixed box body 3 in a sliding mode, the vibrating box body 1 can slide up and down in the fixed box body 3 in a reciprocating mode, and two partition plates 25 which divide the inner space of the vibrating box body 1 into a plurality of dissolving chambers 26 are fixedly installed in the vibrating box body 1 in the height direction; the top of the cylinder 24 is positioned right above the vibration box body 1 and is fixedly connected with the liquid inlet device, and the bottom of the cylinder 24 penetrates through the bottom of the vibration box body 1 and the bottom of the fixed box body 3 and is fixedly connected with the oscillation device; the cylinder 24 is fixedly connected with the vibration box body 1, and the cylinder 24 is connected with the fixed box body 3 in a sliding manner, so that the cylinder 24 can synchronously slide along with the vibration box body 1 when reciprocating up and down; the liquid inlet device is positioned in the cylinder body 24; the liquid inlet end of the liquid discharging device is communicated with the bottoms of the plurality of dissolving chambers 26, the liquid discharging device is used for placing the dissolved cell culture medium into the liquid accumulating device, and the liquid outlet end of the liquid discharging device penetrates through the bottom of the fixed box body 3 and is connected with the liquid accumulating device; the liquid accumulating device and the oscillating device are both fixedly connected with the base 8;
as shown in fig. 3, the liquid inlet device comprises a movable liquid inlet pipe 35, a venturi liquid separating head I34, a plurality of liquid outlet branch pipes 29 and a plurality of electromagnetic valves I33; the top of the first Venturi liquid separation head 34 is fixedly connected with the bottom of the movable liquid inlet pipe 35; the movable liquid inlet pipe 35 is positioned in the liquid supply device and is connected with the liquid supply device in a sliding way; the inner diameter of the movable liquid inlet pipe 35 is gradually increased towards the direction far away from the Venturi liquid separating head I34, so that the resistance of liquid entering the movable liquid inlet pipe 35 can be reduced; a plurality of liquid outlet branch pipes 29 are simultaneously connected with the liquid outlet end at the bottom of the first Venturi liquid separating head 34; the end parts of the liquid outlet branch pipes 29 far away from the Venturi liquid separating head I34 penetrate through the side wall of the cylinder body 24, and the liquid outlet ends of the liquid outlet branch pipes 29 are communicated with the dissolving chambers 26; the first electromagnetic valves 33 are respectively and fixedly arranged on the liquid outlet branch pipes 29, the installation mode adopts a conventional electromagnetic valve installation mode, and the main function is to independently control the opening and closing of each liquid outlet branch pipe 29;
as shown in fig. 1, the liquid discharge device comprises three liquid discharge branch pipes 30, three electromagnetic valves two 2, a venturi liquid separation head two 4 and a liquid discharge main pipe 5; the tops of the three liquid discharge branch pipes 30 are simultaneously communicated with the bottom of the dissolving chambers 26, the bottoms of the three liquid discharge branch pipes 30 penetrate through the bottom of the fixed box body 3 and are simultaneously connected with the top of the second Venturi liquid separation head 4, and the second Venturi liquid separation head 4 is used for confluence, so that the cell culture medium solution in each dissolving chamber 26 can flow into the collecting device clamped by the liquid accumulating device; the bottom of the second Venturi liquid separation head 4 is fixedly connected with the top of the liquid discharge main pipe 5, and the liquid discharge main pipe 5 is in sliding connection with the liquid accumulating device, so that the whole liquid discharging device can also synchronously move when the vibration box body 1 reciprocates up and down; the three electromagnetic valves II 2 are respectively arranged on the three liquid discharge branch pipes 30 in the same way as the conventional installation way, and the main purpose of the three electromagnetic valves II is to independently discharge the cell culture medium solution in the three dissolving chambers 26;
the oscillating device comprises a limiting plate 22, a first spring 23, a second bracket 21, a roller 20, a rotating motor 13, a first rotating shaft 12, a limiting block 11 and a cam 10; as shown in fig. 1, the top of the limiting plate 22 is fixedly connected with the bottom of the cylinder 24; the first spring 23 is sleeved on the barrel 24, and two ends of the first spring 23 are respectively fixedly connected with the bottom of the fixed box body 3 and the top of the limiting plate 22; the top of the second support 21 is fixedly connected with the bottom of the limiting plate 22, the roller 20 is sleeved on the second support 21 in a conventional manner, and the main purpose is to enable the roller 20 to roll around the cross shaft section of the second support 21 and enable the rolling surface of the roller 20 to be in contact with the rolling surface of the cam 10; cam 10 is fixed cup joints on pivot one 12, as shown in fig. 1, the tip of pivot one 12 and the output fixed connection of rotating electrical machines 13, the bottom of rotating electrical machines 13 and stopper 11 all with the top fixed connection of base 8, stopper 11 cup joints on pivot one 12, and stopper 11 and pivot one 12 swing joint, stopper 11 plays the effect of support to pivot one 12 can rotate in stopper 11.
As shown in fig. 1, the liquid supply device includes two liquid supply tubes 28, a particle hopper 27, a pump body 14, a serum storage device, a first flow control valve 17 and a pure water storage tank 16; the liquid inlet and outlet ends of the pump body 14 are respectively communicated with two liquid supply pipes 28; as shown in fig. 2, the end of the liquid supply tube 28 connected to the outlet of the pump body 14 is fixedly connected to the top of the fixed box 3, and the movable liquid inlet tube 35 is located on the liquid supply tube 28 and can slide along the inner wall of the liquid supply tube 28; the serum storage device and the particle funnel 27 are respectively communicated with two liquid supply tubes 28; the end of the liquid supply pipe 28 connected with the liquid inlet end of the pump body 14 is communicated with the pure water storage tank 16, and the pure water storage tank 16 adopts a conventional water tank; the first flow control valve 17 is arranged on a liquid supply pipe 28 connected with the pure water storage tank 16, and the first flow control valve 17 is positioned between the serum storage device and the pure water storage tank 16 and mainly used for controlling the consumption of the pure water; a shielding device is installed in the particle hopper 27.
As shown in fig. 3 and 4, the shielding device includes a stepping motor 36, a bearing 37, a second rotating shaft 32 and two baffles 31; the stepping motor 36 and the bearing 37 are fixedly connected with the outer side wall of the particle funnel 27, the second rotating shaft 32 and the two baffles 31 are positioned in the particle funnel 27, and two ends of the second rotating shaft 32 are respectively fixedly connected with the output end of the stepping motor 36 and the bearing 37; the two baffles 31 are both semicircular, the straight edges of the two baffles 31 are fixedly connected with the opposite positions of the second rotating shaft 32 at the same time, the arc edges of the two baffles 31 are in contact with the inner side wall of the particle hopper 27, and the shielding device in fig. 3 and 4 is in a closed state.
As shown in fig. 1, the serum storage device comprises a serum storage tank 19, a liquid outlet pipe 18 and a second flow control valve 15; the bottom of the serum storage box 19 is connected with the liquid outlet pipe 18, the second flow control valve 15 is installed on the liquid outlet pipe 18, the liquid outlet pipe 18 is communicated with the liquid supply pipe 28, and the second flow control valve 15 is installed in a conventional installation mode and is mainly used for controlling the serum consumption.
The liquid accumulation device comprises a liquid accumulation box body 9, a fixed pipe 6 and two fixed devices; the bottom of the fixed pipe 6 is fixedly connected with the top of the effusion box body 9, the bottom of the liquid drainage main pipe 5 is positioned in the fixed pipe 6, and the liquid drainage main pipe 5 is connected with the fixed pipe 6 in a sliding way; two fixing device slidable mounting are in hydrops box 9 both sides, and two fixing device along the axis position mirror symmetry distribution of hydrops box 9.
As shown in fig. 5, the fixing device comprises a handle 40, a second spring 39, at least two sliding rods 41, an arc-shaped fixing plate 43 and an arc-shaped rubber pad 42; the arc-shaped rubber pad 42 is attached to the arc-shaped fixing plate 43, the side wall of the arc-shaped fixing plate 43, which is far away from the arc-shaped rubber pad 42, is fixedly connected with the second spring 39 and the sliding rod 41, the end part of the second spring 39, which is far away from the side wall of the arc-shaped fixing plate 43, is fixedly connected with the inner side wall of the effusion box body 9, and the end part of the sliding rod 41, which is far away from the arc-shaped fixing plate 43, is fixedly connected with the handle 40.
The working principle is as follows: when an operator needs to prepare the serum-free cell culture medium, setting is carried out through a control panel (not marked in the figure), the control panel transmits information to a control processor (not marked in the figure), the control processor regulates the pump body 14 to be opened, and regulates the first flow control valve 17 to be in a specified state, and the first electromagnetic valve 33 is completely opened; at the moment, the pure water is pumped out of the pure water storage tank 16, and simultaneously, the stepping motor 36 drives the two baffles 31 to rotate, so that the baffles 31 incline, the powder raw materials in the particle funnel 27 fall into the liquid supply pipe 28 to be mixed with the pure water and flow into the position of the first venturi liquid separation head 34 along with the pure water, and the solution is evenly distributed into 3 dissolving chambers 26 under the action of the first venturi liquid separation head 34; in the dissolving stage, the control processor adjusts the rotation of the rotating motor 13, the rotating motor 13 drives the rotating shaft I12 and the cam 10 to rotate, the roller 20 and the cam 10 rotate relatively, meanwhile, under the action of the cam 10, the roller 20 can drive the cylinder 24 and the vibration box body 1 to do high-frequency up-and-down reciprocating motion, so that the powder raw materials and the pure water are fully dissolved and mixed, and finally, each electromagnetic valve II 2 is opened respectively to discharge the cell culture medium solution in each dissolving chamber 26 respectively; when the cell culture medium used in the cell adaptation stage needs to be prepared, each electromagnetic valve I33 needs to be opened independently, the mixing proportion of the electromagnetic valve I and pure water is controlled by controlling the flow control valve II 15, so that the serum concentration in each dissolving chamber 26 is different, and the cell culture medium used in the cell adaptation stage with different serum concentrations can be obtained when the cell culture medium is subjected to shaking dissolving; when the cell culture medium solution with different serum concentrations is discharged, the cell culture medium solution needs to be respectively placed into different containers, and each electromagnetic valve II 2 needs to be opened independently; putting the cell culture medium solutions with different serum concentrations into different collecting devices; the collecting device can adopt a beaker 38 as shown in figure 5, and can also adopt a container with an uneven base 8, such as a test tube; when the collecting device is replaced, an operator pulls the handle 40 to loosen the arc-shaped fixing plate 43 and the arc-shaped rubber pads 42 from the collecting device, and when another container is replaced, the handle 40 is loosened to enable the two arc-shaped rubber pads 42 to be tightly attached to the outer side wall of the beaker 38, so that the effect of fixing the collecting device is achieved; the device can produce serum-free cell culture medium in batch, and can prepare culture medium used in cell adaptation stages with different serum concentrations simultaneously.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (6)
1. Serum-free raw materials dissolving device for cell culture medium configuration, including base (8), characterized by: the top of the base (8) is fixedly provided with a liquid supply device and a dissolving device; the liquid supply device is connected with the top of the dissolving device;
the dissolving device comprises a fixed box body (3), a vibrating box body (1), a plurality of first supports (7), a cylinder body (24), a liquid inlet device, a liquid discharge device, a vibration device and a liquid accumulation device; the bottom of the fixed box body (3) is fixedly connected with the first supports (7) at the same time, and the bottoms of the first supports (7) are fixedly connected with the top of the base (8) at the same time; the vibration box body (1) is positioned in the fixed box body (3), the outer side wall of the vibration box body (1) is connected with the inner side wall of the fixed box body (3) in a sliding manner, and a partition plate (25) for dividing the inner space of the vibration box body (1) into a plurality of dissolving chambers (26) is fixedly arranged in the vibration box body (1); the top of the cylinder body (24) is positioned right above the vibration box body (1) and is connected with the liquid inlet device, and the bottom of the cylinder body (24) penetrates through the bottom of the vibration box body (1) and the bottom of the fixed box body (3) and is connected with the oscillation device; the cylinder (24) is fixedly connected with the vibration box body (1), and the cylinder (24) is slidably connected with the fixed box body (3); the liquid inlet device is positioned in the cylinder body (24); the liquid inlet end of the liquid discharging device is communicated with the bottoms of the plurality of dissolving chambers (26), and the liquid outlet end of the liquid discharging device penetrates through the bottom of the fixed box body (3) and is connected with the liquid accumulating device; the liquid accumulation device and the oscillating device are both connected with the base (8);
the liquid inlet device comprises a movable liquid inlet pipe (35), a first Venturi liquid separating head (34), a plurality of liquid outlet branch pipes (29) and a plurality of first electromagnetic valves (33); the top of the Venturi liquid separation head I (34) is fixedly connected with the bottom of the movable liquid inlet pipe (35); the movable liquid inlet pipe (35) is connected with the liquid supply device in a sliding way; the liquid outlet branch pipes (29) are simultaneously connected with the liquid outlet end at the bottom of the Venturi liquid separating head I (34); the end parts of the liquid outlet branch pipes (29) far away from the Venturi liquid distribution head I (34) penetrate through the side wall of the barrel body (24), and the liquid outlet ends of the liquid outlet branch pipes (29) are communicated with the dissolving chambers (26); the electromagnetic valves I (33) are fixedly arranged on the liquid outlet branch pipes (29) respectively;
the liquid drainage device comprises a plurality of liquid drainage branch pipes (30), a plurality of electromagnetic valves II (2), a Venturi liquid separation head II (4) and a liquid drainage main pipe (5); the tops of the plurality of liquid discharge branch pipes (30) are simultaneously communicated with the bottom of the dissolving chamber (26), and the bottoms of the plurality of liquid discharge branch pipes (30) penetrate through the bottom of the fixed box body (3) and are simultaneously connected with the top of the Venturi liquid separation head II (4); the bottom of the Venturi liquid separation head II (4) is fixedly connected with the top of the liquid drainage main pipe (5), and the liquid drainage main pipe (5) is movably connected with the liquid accumulation device; the second electromagnetic valves (2) are respectively arranged on the liquid discharge branch pipes (30);
the oscillating device comprises a limiting plate (22), a first spring (23), a second bracket (21), a roller (20), a rotating motor (13), a first rotating shaft (12), a limiting block (11) and a cam (10); the top of the limiting plate (22) is fixedly connected with the bottom of the barrel body (24); the first spring (23) is sleeved on the barrel (24), the top of the first spring (23) is fixedly connected with the bottom of the fixed box body (3), and the bottom of the first spring (23) is fixedly connected with the top of the limiting plate (22); the top of the second support (21) is fixedly connected with the bottom of the limiting plate (22), the roller (20) is sleeved on the second support (21), and the rolling surface of the roller (20) is in contact with the rolling surface of the cam (10); the cam (10) is sleeved on the first rotating shaft (12), the end part of the first rotating shaft (12) is fixedly connected with the output end of the rotating motor (13), the bottoms of the rotating motor (13) and the limiting block (11) are fixedly connected with the top of the base (8), the limiting block (11) is sleeved on the first rotating shaft (12), and the limiting block (11) is movably connected with the first rotating shaft (12).
2. The apparatus for dissolving a raw material for preparing a serum-free cell culture medium according to claim 1, wherein: the liquid supply device comprises two liquid supply pipes (28), a particle funnel (27), a pump body (14), a serum storage device, a first flow control valve (17) and a pure water storage tank (16); the liquid inlet and outlet ends of the pump body (14) are respectively connected with two liquid supply pipes (28); the end part of a liquid supply pipe (28) connected with a liquid outlet of the pump body (14) is fixedly connected with the top of the fixed box body (3), the movable liquid inlet pipe (35) is positioned in the liquid supply pipe (28), and the liquid supply pipe (28) is in sliding connection with the movable liquid inlet pipe (35); the serum storage device and the particle funnel (27) are respectively connected with the side walls of the two liquid supply pipes (28); the end part of a liquid supply pipe (28) connected with the liquid inlet end of the pump body (14) is connected with a pure water storage tank (16); the first flow control valve (17) is arranged on a liquid supply pipe (28) connected with the pure water storage tank (16), and the first flow control valve (17) is positioned between the serum storage device and the pure water storage tank (16); a shielding device is arranged in the particle funnel (27).
3. The apparatus for dissolving a raw material for preparing a serum-free cell culture medium according to claim 2, wherein: the shielding device comprises a stepping motor (36), a bearing (37), a second rotating shaft (32) and two baffles (31); the stepping motor (36) and the bearing (37) are fixedly connected with the outer side wall of the particle funnel (27), the second rotating shaft (32) and the two baffles (31) are both positioned in the particle funnel (27), and two ends of the second rotating shaft (32) are respectively fixedly connected with the output end of the stepping motor (36) and the bearing (37); two baffle (31) are semi-circular, and two the straight line limit of baffle (31) is simultaneously with second (32) fixed connection of pivot, two the arc line edge of baffle (31) contacts with the inside wall of granule funnel (27).
4. The apparatus for dissolving a raw material for preparing a serum-free cell culture medium according to claim 2, wherein: the serum storage device comprises a serum storage tank (19), a liquid outlet pipe (18) and a second flow control valve (15); the bottom and drain pipe (18) of serum bin (19) are connected, install on drain pipe (18) flow control valve two (15), drain pipe (18) and feed pipe (28) intercommunication.
5. The apparatus for dissolving a raw material for preparing a serum-free cell culture medium according to claim 1, wherein: the liquid accumulation device comprises a liquid accumulation box body (9), a fixed pipe (6) and two fixing devices; the bottom of the fixed pipe (6) is fixedly connected with the top of the effusion box body (9), the bottom of the liquid drainage main pipe (5) is positioned in the fixed pipe (6), and the liquid drainage main pipe (5) is connected with the fixed pipe (6) in a sliding manner; two fixing device slidable mounting is in hydrops box (9) both sides, and two fixing device follows the axis position mirror symmetry distribution of hydrops box (9).
6. The apparatus for dissolving a raw material for preparing a serum-free cell culture medium according to claim 5, wherein: the fixing device comprises a handle (40), a second spring (39), at least two sliding rods (41), an arc-shaped fixing plate (43) and an arc-shaped rubber pad (42); arc rubber pad (42) laminate on arc fixed plate (43), the lateral wall that arc rubber pad (42) was kept away from in arc fixed plate (43) simultaneously with two (39) springs and slide bar (41) fixed connection, the tip that arc fixed plate (43) lateral wall was kept away from in two (39) springs and the inside wall fixed connection of hydrops box (9), the tip and handle (40) fixed connection of arc fixed plate (43) are kept away from in slide bar (41).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210515529.1A CN114832655A (en) | 2022-05-11 | 2022-05-11 | Raw material dissolving device for preparing serum-free cell culture medium |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210515529.1A CN114832655A (en) | 2022-05-11 | 2022-05-11 | Raw material dissolving device for preparing serum-free cell culture medium |
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| CN114832655A true CN114832655A (en) | 2022-08-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202210515529.1A Pending CN114832655A (en) | 2022-05-11 | 2022-05-11 | Raw material dissolving device for preparing serum-free cell culture medium |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117563455A (en) * | 2024-01-17 | 2024-02-20 | 广东奇乐趣食品科技有限公司 | Compound cake emulsifier improves effect mixing arrangement |
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