CN114762805A - Full-automatic combined blending method and device - Google Patents

Abstract

The invention is a fully automatic combined mixing method and a device thereof, which adopts two mixing methods and a mixing device of non-contact vibration mixing and sampling needle mixing, and sequentially performs two different mixing treatment methods on the reaction reagents. . Oscillation mixing is to use the eccentric structure to make the reaction cup or reagent chip reciprocate in the horizontal direction during the reciprocating movement in the horizontal direction by using the eccentric structure, so that the liquid in the reaction cup will change. Stir up and down to make the solution relatively uniform. When the sampling needle is mixed, the liquid is directly stirred with the sampling needle to accelerate the relative movement between the solutions, so that the solution can achieve a relatively uniform effect. The combination of the two methods not only improves the mixing efficiency of the reaction reagents, but also solves the technical problems that the mixing devices in the existing in vitro diagnostic instruments have a narrow scope of application, cannot take into account multiple liquid-phase reagents, and have poor mixing effects.

Description

A fully automatic combined mixing method and device thereof

technical field

The present application relates to the technical field of biochemical mixing of in vitro diagnostic instruments, in particular to a fully automatic combined mixing method and device thereof.

Background technique

In the current field of biochemical technology, in vitro diagnostic instruments using liquid-phase reagents all require a mixing structure to mix reagents and samples or between reagents and reagents. The quality of the mixing effect directly affects the stability of the reagent reaction. , which in turn affects the reliability of the test results.

The existing mixing technologies mainly include ultrasonic mixing, vibration mixing, stirring rod mixing, etc., but the above mixing methods each have their own shortcomings. The ultrasonic mixing structure is large in volume, high in cost, and easy to generate bubbles in the liquid; the mixing efficiency is low, and the mixing effect for liquids with high viscosity is poor, and the amount of liquid is limited; the mixing method of stirring rod requires a special stirring rod for mixing. Stirring, but also a special cleaning mechanism to clean the stirring rod.

SUMMARY OF THE INVENTION

The homogenizing module performs stirring and mixing treatment on the liquid-phase reagents in the reaction device;

A vibrating and mixing module, which vibrates and mixes the liquid-phase reagents in the reaction device;

The mobile module is installed between the vibration mixing module and the sampling needle mixing module, and the reaction device is carried out between the sampling needle mixing module and the vibration mixing module transfer.

Specifically, the sampling needle mixing module is installed on the sampling needle mixing station.

Specifically, the shaking and mixing module is installed on the shaking and mixing station.

Specifically, the mobile module is installed on the mobile station.

Specifically, the moving module may be a guide rail driving device, a conveyor belt driving device, a rotating shaft driving device, etc., which are used to drive the movement of the reaction device.

Specifically, there is no order of work between the sampling needle mixing station and the oscillating mixing station. Either the sampling needle mixing station can be completed first, or the oscillating mixing station can be completed first.

Specifically, the reaction device may be a reaction device such as a reaction cup, a reagent chip cartridge, or the like.

The present invention adopts two mixing methods and mixing devices of non-contact vibration mixing and sampling needle mixing, and sequentially performs two different mixing treatment methods on the reaction reagent. Non-contact oscillating mixing is to use the eccentric structure to make the cuvette or reagent chip reciprocate in the horizontal direction under the condition that the running direction of the motor remains unchanged. During the reciprocating motion of the cuvette, there will be changes in the direction of movement and acceleration and deceleration , the inertia of the movement of the object makes the liquid in the reaction cup toss up and down, so that the solution achieves a relatively uniform effect. When the sampling needle is mixed, the liquid is directly stirred with the sampling needle, which accelerates the relative movement between the solutions, accelerates the speed of diffusion and dissolution, and makes the solution relatively uniform. The combination of the two methods not only improves the mixing efficiency of the reaction reagents, but also solves the technical problems that the existing mixing instruments have a narrow scope of application, cannot take into account a variety of liquid-phase reagents, and have poor mixing effects.

The sampling needle mixing module includes a sampling needle, a vibration motor and a vibration motor base; the vibration motor base is respectively connected with the sampling needle and the vibration motor, and the vibration motor transmits the vibration to the sampling needle through the vibration motor base.

Specifically, when the sample or reagent is added to the reaction device by the sampling needle, the vibration motor can be activated, and the vibration caused by the rotation of the motor is transmitted to the sampling needle and can generate a relatively obvious amplitude at the tip of the sampling needle through the amplification effect of the needle body.

It should be noted that the sampling needle takes into account the functions of sampling and stirring at this time, which not only saves consumables, but also saves the cleaning of general stirring rods.

Further, the vibration frequency and amplitude of the sampling needle can be adjusted by adjusting the working frequency of the vibration motor, and the working frequency of the vibration motor can be determined according to the actual mixing effect. Different vibration frequencies can be used for different reagents.

The vibration mixing module includes: mixing pulling mechanism, mixing component base and mixing motor; the mixing motor is installed at the lower part of the mixing component base, and the mixing pulling mechanism is installed on the side of the mixing component base; mixing pulling material The mechanism moves the reaction device to the base of the mixing component, and the mixing motor drives the reaction device to move.

The mixing motor includes: a motor shaft, a mixing mechanism, and a rolling bearing; the motor shaft and the rolling bearing are engaged with a mixing mechanism, and the mixing mechanism drives the reaction device to perform eccentric motion, circular motion or reciprocating motion.

Specifically, the mixing mechanism may be a mixing eccentric shaft, which drives the reaction device to perform horizontal reciprocating eccentric motion.

Alternatively, the mixing mechanism may be a central shaft, which drives the reaction device to perform horizontal circular motion.

Alternatively, the mixing motor can directly switch the movement direction of the motor to drive the cuvette to reciprocate.

The mixing component base includes: parallel guide rails, chip mixing seat, and chip mixing groove; parallel guide rails are installed on the chip mixing seat, and the chip mixing groove can slide on the parallel guide rails. The opening is connected to the mixing motor.

Specifically, there are two parallel guide rails.

The mixing and pulling mechanism includes: the bottom plate of the mixing component, the mixing pulling motor, the mixing pulling guide and the mixing chip pulling rod; the installation of the mixing pulling motor, the mixing pulling guide and the mixing chip pulling rod On the bottom plate of the mixing assembly, the mixing chip pulling rod is installed on the mixing pulling guide rail, and the mixing pulling motor drives the mixing chip pulling rod to move on the mixing pulling guide rail through the transmission belt to pull the reaction device. into the chip mixing tank.

The mixing and pulling mechanism also includes a spring, and the spring is installed between the bottom plate of the mixing component and the chip mixing tank.

Specifically, a spring is also designed on the bottom plate of the mixing assembly, under the action of the spring, the noise caused by the gap between the guide rails can be reduced, and at the same time, the chip mixing seat can be kept in a fixed position when the mixing motor is powered off.

Further, the present invention provides an in vitro diagnostic instrument for liquid-phase reagents, using the following fully automatic combined mixing method:

The sampling needle mixing station is used to stir and mix the liquid-phase reagents in the reaction device;

The vibrating and mixing station is for vibrating and mixing the liquid-phase reagents;

The moving station transfers the reaction device between the sampling needle mixing station and the shaking mixing station.

Further, the present invention provides an in vitro diagnostic instrument for liquid-phase reagents, which adopts the following fully automatic combined mixing device:

The sampling needle mixing module is used for stirring and mixing the liquid-phase reagents in the reaction device;

The vibrating and mixing module is used for vibrating and mixing the liquid-phase reagents in the reaction device;

The moving module is installed between the vibration mixing module and the sampling needle mixing module, and the reaction device is transferred between the sampling needle mixing module and the vibration mixing module.

The invention provides an in-vitro diagnostic instrument for liquid-phase reagents. The instrument adopts two mixing methods and mixing devices of non-contact vibration mixing and sampling needle mixing, and respectively stirs and mixes the liquid-phase reagents in the reaction device. Homogenization treatment and oscillating mixing treatment; to solve the technical problems that the existing mixing technology has a narrow scope of application, cannot take into account a variety of liquid-phase reagents, and has poor mixing effect.

Description of drawings

1 is a schematic diagram of a framework of a fully automatic combined mixing method provided in an embodiment;

2 is a structural diagram of a fully automatic combined mixing device provided in an embodiment;

3 is a schematic structural diagram of a sampling needle mixing module provided in an embodiment;

FIG. 4 is a schematic structural diagram of an oscillation mixing module provided by an embodiment.

Detailed ways

In order to make the purpose, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings and embodiments; obviously, the described embodiments are only part of the implementation of the present invention. Examples are not all embodiments; based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention.

Example 1:

Referring to Figure 1, the present invention provides a fully automatic combined mixing method: the liquid-phase reagents in the reaction device are stirred and mixed at the sampling needle mixing station; station, and the liquid-phase reagent is shaken and mixed evenly.

Example 2:

Referring to Figure 1, the present invention provides a fully automatic combined mixing method: vibrating and mixing the liquid-phase reagents at the shaking mixing station, and then moving the reaction device to the sampling needle mixing station through the moving station. , the liquid-phase reagents in the reaction device are stirred and mixed evenly.

Example 3:

Referring to Fig. 2, Fig. 3 and Fig. 4, the invention provides a fully automatic combined mixing device, comprising: a sampling needle mixing module as shown in Fig. 3 and a vibration mixing module as shown in Fig. 4, including a mixing motor 1, The mixing assembly base plate 11 is installed on the mixing assembly base plate 11 with the mixing assembly base 4 , the chip mixing seat 6 , and the mixing and pulling motor 8 .

A mixing eccentric shaft 2 and a rolling bearing 3 are installed on the shaft of the mixing motor 1 to form an eccentric structure; two parallel guide rails 5 are installed on the mixing component base 4, and the chip mixing seat 6 can move horizontally on the guide rails 5, and the chip There is an opening in the middle of the mixing seat 6, and the mixing motor 1 is connected to the opening on the chip mixing seat 6 through the rolling bearing 3. The bottom 11 of the mixing assembly is also designed with a spring 10, which can reduce parallelism under the action of the spring 10. The noise caused by the gap of the guide rails 5 can also make the mixing motor 1 power off, the chip mixing seat 6 can be kept in a fixed position.

The mixing and pulling mechanism includes a mixing pulling motor 8, a mixing pulling guide 12, and a mixing chip pulling rod 9. This mechanism can pull the reaction cup or reagent chip that needs to be mixed into the chip mixing seat. 6.

The sampling needle mixing mechanism mainly includes a sampling needle 1 , a vibration motor 2 , and a vibration motor base 3 . The vibration motor base 3 is respectively connected to the sampling needle 1 and the vibration motor 2. When the vibration motor 2 is working, the vibration is transmitted to the sampling needle 1 through the vibration motor base 3, and the sampling needle 1 stirs the liquid to achieve a mixing effect.

When the sample or reagent is added to the cuvette by the sampling needle 1, the vibration motor 2 can be started, and the vibration caused by the rotation of the vibration motor 2 is transmitted to the sampling needle 1 and can produce obvious vibration at the needle tip of the sampling needle 1 through the amplification effect of the needle body. The amplitude, frequency and amplitude of vibration can be adjusted by adjusting the working frequency of the vibration motor 2, and the working frequency of the vibration motor 2 can be determined according to the actual mixing effect.

The reaction cup or reagent chip after sample addition or reagent addition is moved from the mixing chip pulling rod 7 to the chip mixing seat 6. The mixing motor 1 drives the chip mixing seat 6 to move through the eccentric structure, and the chip mixing seat 6 moves. 6 When the horizontal reciprocating motion drives the chips inside it to move at the same time, the liquid in the reaction cup is tossed up and down due to the speed change to achieve the effect of mixing.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalents, and improvements made within the spirit and principles of the present invention shall be included in the present invention. within the scope of protection.

Claims (10)

1. a fully automatic joint mixing method, is characterized in that, comprises: The sampling needle mixing station is used to stir and mix the reagents in the reaction device; The shaking and mixing station is used for shaking and mixing the reagents; The sampling needle mixing station is arranged on the side of the vibration mixing station, and the sampling needle mixing station and the vibration mixing station have no sequence in the working process. 2. The mixing module, the sampling needle mixing module performs stirring and mixing treatment on the liquid-phase reagents in the reaction device; A vibrating and mixing module, which vibrates and mixes the liquid-phase reagents in the reaction device; The mobile module is installed between the vibration mixing module and the sampling needle mixing module, and the reaction device is carried out between the sampling needle mixing module and the vibration mixing module transfer. 3. fully automatic joint mixing device as claimed in claim 2, is characterized in that, described sampling needle mixing module comprises sampling needle, vibration motor and vibration motor seat; Described vibration motor seat is connected with described sampling needle respectively and the vibration motor, which transmits vibration to the sampling needle through the vibration motor base. 4. The fully automatic combined mixing device according to claim 2, wherein the vibration mixing module comprises: a mixing pulling mechanism, a mixing assembly base, a mixing mechanism and a mixing motor; the The mixing motor is installed at the lower part of the base of the mixing assembly, and the mixing and pulling mechanism is installed on the side of the base of the mixing assembly; the mixing pulling mechanism moves the reaction device to the base of the mixing assembly On the top, the mixing motor drives the mixing mechanism to move. 5. The fully automatic combined mixing device according to claim 4, wherein the mixing motor comprises: a motor shaft, a mixing mechanism and a rolling bearing; the motor shaft and the rolling bearing are engaged with the mixing The mixing mechanism drives the reaction device to perform eccentric motion, circular motion or reciprocating motion. 6. The fully automatic combined mixing device according to claim 4, wherein the mixing assembly base comprises: a parallel guide rail, a chip mixing seat and a chip mixing groove; The parallel guide rail, the chip mixing groove slides on the parallel guide rail, and the chip mixing seat is provided with an opening, and is connected to the mixing motor through the opening. 7. The fully automatic combined mixing device according to claim 6, wherein the mixing and pulling mechanism comprises: a mixing assembly bottom plate, a mixing and pulling motor, a mixing and pulling guide rail, and a mixing chip puller. material rod; the mixing pulling motor, the mixing pulling guide rail and the mixing chip pulling rod are installed on the bottom plate of the mixing assembly, and the mixing chip pulling rod is installed on the mixing On the material mixing guide rail, the mixing pulling motor drives the mixing chip pulling rod to move on the mixing pulling guide rail through a transmission belt, so as to pull the reaction device into the chip mixing tank. 8. The fully automatic combined mixing device according to claim 6, wherein the mixing and pulling mechanism further comprises a spring, and the spring is installed between the mixing assembly bottom plate and the chip mixing tank. between. 9 . An in vitro diagnostic instrument for liquid-phase reagents, characterized in that the fully automatic combined mixing method according to claim 1 is used. 10 . 10 . An in vitro diagnostic instrument for liquid-phase reagents, characterized in that any fully automatic combined mixing and homogenizing device as claimed in claims 2 to 8 is used. 11 .

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