CN114762805A - Full-automatic combined blending method and device - Google Patents
Full-automatic combined blending method and device Download PDFInfo
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- CN114762805A CN114762805A CN202011628293.XA CN202011628293A CN114762805A CN 114762805 A CN114762805 A CN 114762805A CN 202011628293 A CN202011628293 A CN 202011628293A CN 114762805 A CN114762805 A CN 114762805A
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- blending
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- sampling needle
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- 238000002156 mixing Methods 0.000 title claims abstract description 245
- 238000000034 method Methods 0.000 title claims abstract description 13
- 238000005070 sampling Methods 0.000 claims abstract description 49
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 41
- 230000010355 oscillation Effects 0.000 claims abstract description 35
- 239000007791 liquid phase Substances 0.000 claims abstract description 22
- 238000003756 stirring Methods 0.000 claims abstract description 16
- 238000000338 in vitro Methods 0.000 claims abstract description 8
- 230000008569 process Effects 0.000 claims abstract description 3
- 230000007246 mechanism Effects 0.000 claims description 24
- 239000000463 material Substances 0.000 claims description 21
- 238000005096 rolling process Methods 0.000 claims description 5
- 238000000265 homogenisation Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 18
- 239000007788 liquid Substances 0.000 abstract description 9
- 230000008859 change Effects 0.000 abstract description 3
- 230000001133 acceleration Effects 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 230000003321 amplification Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000003805 vibration mixing Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to a full-automatic combined blending method and a device thereof, which adopt two blending modes of non-contact oscillation blending and sampling needle blending and a blending device to sequentially carry out two different blending treatment modes on a reaction reagent. The oscillation mixing is that under the condition that the running direction of the eccentric structure in the direction of the motor is not changed, the movement direction and the movement acceleration and deceleration change in the process that the reaction cup or the reagent chip performs reciprocating movement in the horizontal direction, so that the liquid in the reaction cup is overturned up and down to further enable the solution to achieve the relatively uniform effect. And the sampling needle is directly used for stirring the liquid after uniformly mixing the sampling needles, so that the relative movement between the solutions is accelerated, and the solution can achieve the relatively uniform effect. The combination of the two modes not only improves the mixing efficiency of the reaction reagent, but also solves the technical problems that the mixing device in the existing in-vitro diagnostic instrument has narrow application range, cannot give consideration to various liquid phase reagents and has poor mixing effect.
Description
Technical Field
The application relates to the technical field of biochemical mixing of in-vitro diagnostic instruments, in particular to a full-automatic combined mixing method and a device thereof.
Background
In the existing biochemical technical field, the in vitro diagnostic apparatus using liquid phase reagent needs to have a mixing structure to mix the reagent and the sample or the reagent and the reagent, and the quality of the mixing effect directly affects the stability of the reagent reaction, and further affects the reliability of the test result.
The existing blending technology mainly comprises the modes of ultrasonic blending, vibration blending, stirring rod blending and the like, but the blending modes have the defects. The ultrasonic mixing structure has large volume and high cost, and is easy to cause the liquid to generate bubbles; the oscillating and mixing efficiency is low, the mixing effect on liquid with high viscosity is poor, and the liquid amount is limited; the mixing mode of the stirring rod needs a special stirring rod for stirring and a special cleaning mechanism for cleaning the stirring rod.
Disclosure of Invention
The homogenizing module is used for stirring and uniformly mixing the liquid-phase reagent in the reaction device;
the oscillation blending module is used for carrying out oscillation blending treatment on the liquid phase reagent in the reaction device;
and the moving module is arranged between the oscillation blending module and the sampling needle blending module, and transmits the reaction device between the sampling needle blending module and the oscillation blending module.
Specifically, the sampling needle blending module is installed on the sampling needle blending station.
Specifically, the oscillation blending module is arranged on the oscillation blending station.
Specifically, the mobile module is installed on the mobile station.
Specifically, the moving module may be a module for driving the reaction device to move, such as a guide rail driving device, a conveyor belt driving device, a rotating shaft driving device, and the like.
Specifically, the sampling needle blending station and the oscillation blending station have no working sequence, so that the working of the sampling needle blending station can be completed firstly, and the working of the oscillation blending station can be completed firstly.
Specifically, the reaction apparatus may be a reaction cup, a reagent chip cartridge or the like.
The invention adopts two mixing modes of non-contact oscillation mixing and sampling needle mixing and a mixing device to carry out two different mixing treatment modes on a reaction reagent in sequence. The non-contact oscillation mixing is to make the reaction cup or the reagent chip do reciprocating motion in the horizontal direction under the condition that the running direction of the motor is not changed by utilizing an eccentric structure, the reciprocating motion process of the reaction cup has the change of the motion direction and the motion acceleration and deceleration, and the inertia of the motion of an object makes the liquid in the reaction cup tumble up and down so as to make the solution reach the relatively uniform effect. And the sampling needle is directly used for stirring the liquid after uniformly mixing, so that the relative motion between the solutions is accelerated, the diffusion and dissolution speeds are accelerated, and the solution achieves the relatively uniform effect. The combination of the two modes not only improves the mixing efficiency of the reaction reagent, but also solves the technical problems that the existing mixing instrument has narrow application range, cannot give consideration to various liquid phase reagents and has poor mixing effect.
The sampling needle blending module comprises a sampling needle, a vibration motor and a vibration motor base; the vibration motor base is respectively connected with the sampling needle and the vibration motor, and the vibration motor transmits vibration to the sampling needle through the vibration motor base.
Specifically, after a sample or a reagent is added into the reaction device through the sampling needle, the vibration motor can be started, the vibration caused by the rotation of the motor is transmitted to the sampling needle, and the obvious amplitude can be generated at the needle point of the sampling needle through the amplification effect of the needle body.
It should be noted that, the sampling needle takes into account the effects of sampling and stirring at this time, which not only saves the material consumption, but also saves the cleaning link of the common stirring rod.
Furthermore, the vibration frequency and amplitude of the sampling needle can be adjusted by adjusting the working frequency of the vibration motor, and the working frequency of the vibration motor can be determined according to the actual uniform mixing effect. Different vibration frequencies are adopted for different reaction reagents.
The vibration mixing module includes: the blending and pulling mechanism, the blending component base and the blending motor are arranged on the blending and pulling mechanism; the blending motor is arranged at the lower part of the blending component base, and the blending pulling mechanism is arranged on the side surface of the blending component base; the mixing material pulling mechanism moves the reaction device to the mixing component base, and the mixing motor drives the reaction device to move.
The mixing motor includes: a motor shaft, a blending mechanism and a rolling bearing; the motor shaft and the rolling bearing are clamped with the blending mechanism, and the blending mechanism drives the reaction device to perform eccentric motion, circular motion or reciprocating motion.
Specifically, the blending mechanism may be a blending eccentric shaft, which drives the reaction device to perform a horizontally reciprocating eccentric motion.
Alternatively, the blending mechanism may be a circular mandrel that drives the reaction apparatus to perform a horizontal circular motion.
Alternatively, the mixing motor can be directly used for rapidly switching the motor motion direction to drive the reaction cup to reciprocate.
The mixing subassembly base includes: the chip mixing device comprises parallel guide rails, a chip mixing seat and a chip mixing groove; install parallel guide on the chip mixing seat, the chip mixing groove can slide on parallel guide, sets up the trompil on the chip mixing seat to be connected with the mixing motor through the trompil.
Specifically, the number of the parallel guide rails is two.
Mixing draws material mechanism to include: the mixing assembly comprises a mixing assembly bottom plate, a mixing pulling motor, a mixing pulling guide rail and a mixing chip pulling rod; the mixing draws material motor, mixing to draw the material guide rail and mixing chip to draw the material pole and install on mixing subassembly bottom plate, and mixing chip draws the material pole and installs on mixing draws the material guide rail, and mixing draws the material motor to draw the material pole through drive belt drive mixing chip and draws the material pole and move on mixing draws the material guide rail to draw reaction unit into chip mixing groove.
The mixing pulling mechanism further comprises a spring, and the spring is installed between the mixing component bottom plate and the chip mixing groove.
Specifically, still design on the blending subassembly bottom plate has the spring, and the noise that the reducible guide rail clearance caused under the effect of spring causes also can make the blending motor fall the power failure simultaneously, and chip blending seat can keep in fixed position.
Further, the invention provides an in vitro diagnostic apparatus for liquid phase reagents, which uses the following full-automatic combined blending method:
a sampling needle mixing station for mixing and mixing the liquid phase reagent in the reaction device;
the oscillation blending station is used for carrying out oscillation blending treatment on the liquid-phase reagent;
and the moving station is used for transferring the reaction device between the sampling needle blending station and the oscillation blending station.
Furthermore, the invention provides an in-vitro diagnostic instrument for liquid-phase reagents, which adopts the following full-automatic combined blending device:
the sampling needle blending module is used for stirring and blending the liquid phase reagent in the reaction device;
the oscillation blending module is used for carrying out oscillation blending treatment on the liquid phase reagent in the reaction device;
the moving module is installed between the oscillation blending module and the sampling needle blending module, and transmits the reaction device between the sampling needle blending module and the oscillation blending module.
The invention provides an in-vitro diagnostic instrument for a liquid-phase reagent, which adopts two mixing modes of non-contact oscillation mixing and sampling needle mixing and a mixing device to respectively carry out stirring and mixing treatment and oscillation mixing treatment on the liquid-phase reagent in a reaction device; the technical problems that the application range is narrow, multiple liquid phase reagents cannot be taken into consideration and the mixing effect is poor in the existing mixing technology are solved.
Drawings
Fig. 1 is a schematic frame diagram of a full-automatic combined blending method according to an embodiment;
FIG. 2 is a structural diagram of a fully automatic combined blending device according to an embodiment;
fig. 3 is a schematic structural diagram of a sampling needle blending module according to an embodiment;
fig. 4 is a schematic structural diagram of an oscillating blend mixing module according to an embodiment.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly and completely understood, the following description, with reference to the accompanying drawings and embodiments, makes a clear and complete description of the technical solutions in the embodiments of the present invention; it is to be understood that the described embodiments are merely a subset of the embodiments of the invention, and not all embodiments; all other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Example 1:
referring to fig. 1, the invention provides a full-automatic combined blending method: stirring and uniformly mixing the liquid phase reagent in the reaction device on a sampling needle uniformly mixing station; and then moving the reaction device to an oscillation blending station through a moving station, and carrying out oscillation blending treatment on the liquid-phase reagent.
Example 2:
referring to fig. 1, the invention provides a full-automatic combined blending method: and carrying out oscillation blending treatment on the liquid phase reagent at an oscillation blending station, then moving the reaction device to a sampling needle blending station through a moving station, and carrying out stirring blending treatment on the liquid phase reagent in the reaction device.
Example 3:
referring to fig. 2, 3 and 4, the present invention provides a full-automatic combined blending device, including: the sampling needle blending module shown in fig. 3 and the oscillation blending module shown in fig. 4 comprise a blending motor 1 and a blending assembly bottom plate 11, wherein a blending assembly base 4, a chip blending seat 6 and a blending pulling motor 8 are further arranged on the blending assembly bottom plate 11.
A shaft of the blending motor 1 is provided with a blending eccentric shaft 2 and a rolling bearing 3 to form an eccentric structure; install two parallel guide 5 on mixing subassembly base 4, chip mixing seat 6 can be at guide 5 upper horizontal migration, there is a trompil in the centre of chip mixing seat 6, mixing motor 1 passes through antifriction bearing 3 and is connected with the trompil on the chip mixing seat 6, it has spring 10 still to design on the mixing subassembly bottom plate 11, the noise that the 5 clearances of reducible parallel guide caused under the effect of spring 10, also can make mixing motor 1 fall the power down the time simultaneously, chip mixing seat 6 can keep in fixed position.
The mixing and pulling mechanism comprises a mixing and pulling motor 8, a mixing and pulling guide rail 12 and a mixing chip pulling rod 9, and can realize that a reaction cup or a reagent chip needing to be mixed is pulled into the chip mixing seat 6.
The sampling needle blending mechanism mainly comprises a sampling needle 1, a vibration motor 2 and a vibration motor base 3. Sampling needle 1 and vibrating motor 2 are connected respectively to vibrating motor seat 3, and vibrating motor 2 during operation can transmit the vibration for sampling needle 1 through vibrating motor seat 3, stirs liquid by sampling needle 1 and plays the mixing effect.
After a sample or a reagent is added into the reaction cup through the sampling needle 1, the vibration motor 2 can be started, vibration caused by rotation of the vibration motor 2 is transmitted to the sampling needle 1, and a relatively obvious amplitude can be generated at the needle point of the sampling needle 1 through the amplification effect of the needle body, the frequency and the amplitude of the vibration can be adjusted by adjusting the working frequency of the vibration motor 2, and the working frequency of the vibration motor 2 can be determined according to the actual uniform mixing effect.
The reaction cup or the reagent chip for completing sample adding or reagent adding is moved into the chip mixing seat 6 by the mixing chip pulling rod 7, the mixing motor 1 drives the chip mixing seat 6 to move through an eccentric structure, the chip mixing seat 6 drives the chip inside to move simultaneously when horizontally reciprocating, and liquid in the reaction cup overturns up and down due to speed change to achieve the effect of mixing.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. A full-automatic combined blending method is characterized by comprising the following steps:
a sampling needle mixing station for mixing and mixing the reagent in the reaction device;
the oscillation blending station is used for carrying out oscillation blending treatment on the reagent;
the sampling needle blending station is arranged on the side face of the oscillation blending station, and the sampling needle blending station and the oscillation blending station are not in sequence in the working process.
2. The sampling needle blending module is used for stirring and blending the liquid phase reagent in the reaction device;
the oscillation blending module is used for carrying out oscillation blending treatment on the liquid phase reagent in the reaction device;
and the moving module is arranged between the oscillation blending module and the sampling needle blending module, and transmits the reaction device between the sampling needle blending module and the oscillation blending module.
3. The full-automatic combined blending device of claim 2, wherein the sampling needle blending module comprises a sampling needle, a vibration motor and a vibration motor base; the vibration motor seat is connected respectively the sampling needle with vibration motor, vibration motor passes through vibration motor seat transmits the vibration for the sampling needle.
4. The full-automatic combined blending device of claim 2, wherein the oscillation blending module comprises: the blending and pulling mechanism, the blending component base, the blending mechanism and the blending motor are arranged on the blending component base; the blending motor is arranged at the lower part of the blending component base, and the blending material pulling mechanism is arranged on the side surface of the blending component base; the mixing and pulling mechanism moves the reaction device to the mixing component base, and the mixing motor drives the mixing mechanism to move.
5. The full-automatic combined blending device of claim 4, wherein the blending motor comprises: a motor shaft, a blending mechanism and a rolling bearing; the motor shaft and the rolling bearing are clamped with the blending mechanism, and the blending mechanism drives the reaction device to perform eccentric motion, circular motion or reciprocating motion.
6. The full-automatic combined blending device of claim 4, wherein the blending assembly base comprises: the chip mixing device comprises parallel guide rails, a chip mixing seat and a chip mixing groove; the chip mixing seat is provided with the parallel guide rails, the chip mixing groove slides on the parallel guide rails, and the chip mixing seat is provided with openings and is connected with the mixing motor through the openings.
7. The full-automatic combined blending device of claim 6, wherein the blending pulling mechanism comprises: the mixing assembly comprises a mixing assembly bottom plate, a mixing pulling motor, a mixing pulling guide rail and a mixing chip pulling rod; the mixing draws the material motor, the mixing draws the material guide rail and the mixing chip draws the material pole to install on the mixing subassembly bottom plate, the mixing chip draws the material pole to install on the mixing draws the material guide rail, the mixing draws the material motor to pass through drive belt drive the mixing chip draws the material pole to be in the mixing draws the material guide rail upward movement, with will reaction unit draws in the chip mixing groove.
8. The full-automatic combined blending device of claim 6, wherein the blending pulling mechanism further comprises a spring, and the spring is installed between the blending assembly bottom plate and the chip blending groove.
9. An in vitro diagnostic apparatus for liquid phase reagents, characterized in that the full-automatic combined homogenization method according to claim 1 is used.
10. An in-vitro diagnostic apparatus for liquid-phase reagents, which is characterized by adopting any full-automatic combined blending device as claimed in claims 2 to 8.
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CN202011628293.XA CN114762805A (en) | 2020-12-31 | 2020-12-31 | Full-automatic combined blending method and device |
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CN202011628293.XA CN114762805A (en) | 2020-12-31 | 2020-12-31 | Full-automatic combined blending method and device |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116500249A (en) * | 2023-03-08 | 2023-07-28 | 苏州奎克泰生物技术有限公司 | Detection device for immunodiagnosis |
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CN109116013A (en) * | 2018-09-25 | 2019-01-01 | 珠海丽珠试剂股份有限公司 | A kind of multiplexed immunoassay instrument |
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CN110530703A (en) * | 2019-05-08 | 2019-12-03 | 深圳雷杜生命科学股份有限公司 | A kind of liquid relief agitating device and its stirring quantization method |
CN209784026U (en) * | 2019-02-20 | 2019-12-13 | 重庆科斯迈生物科技有限公司 | Liquid detection analysis mixing system |
CN110736736A (en) * | 2018-07-18 | 2020-01-31 | 博阳生物科技(上海)有限公司 | homogeneous phase chemiluminescence POCT detection method and device using same |
CN212255364U (en) * | 2019-12-19 | 2020-12-29 | 江苏泽成生物技术有限公司 | Full-automatic chemiluminescence immunoassay analyzer |
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2020
- 2020-12-31 CN CN202011628293.XA patent/CN114762805A/en active Pending
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
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CN202289936U (en) * | 2011-08-26 | 2012-07-04 | 杭州瑞诚仪器有限公司 | Oscillator |
CN202614777U (en) * | 2012-06-14 | 2012-12-19 | 长春迪瑞医疗科技股份有限公司 | Transport mechanism for test tube racks |
CN203705458U (en) * | 2013-11-12 | 2014-07-09 | 深圳市国赛生物技术有限公司 | Full-automatic quick testing system for single sample |
CN110736736A (en) * | 2018-07-18 | 2020-01-31 | 博阳生物科技(上海)有限公司 | homogeneous phase chemiluminescence POCT detection method and device using same |
CN109187160A (en) * | 2018-09-20 | 2019-01-11 | 中国科学院苏州生物医学工程技术研究所 | Evenly mixing device |
CN109116013A (en) * | 2018-09-25 | 2019-01-01 | 珠海丽珠试剂股份有限公司 | A kind of multiplexed immunoassay instrument |
CN209784026U (en) * | 2019-02-20 | 2019-12-13 | 重庆科斯迈生物科技有限公司 | Liquid detection analysis mixing system |
CN110530703A (en) * | 2019-05-08 | 2019-12-03 | 深圳雷杜生命科学股份有限公司 | A kind of liquid relief agitating device and its stirring quantization method |
CN212255364U (en) * | 2019-12-19 | 2020-12-29 | 江苏泽成生物技术有限公司 | Full-automatic chemiluminescence immunoassay analyzer |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN116500249A (en) * | 2023-03-08 | 2023-07-28 | 苏州奎克泰生物技术有限公司 | Detection device for immunodiagnosis |
CN116500249B (en) * | 2023-03-08 | 2023-10-20 | 苏州奎克泰生物技术有限公司 | Detection device for immunodiagnosis |
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