CN114306124A

CN114306124A - Supermolecule preparation with antioxidant, anti-sugar and anti-blue-light effects and preparation method thereof

Info

Publication number: CN114306124A
Application number: CN202111602539.0A
Authority: CN
Inventors: 陈斌; 董亮; 王琳
Original assignee: Shanghai Chenyang Biotechnology Co ltd
Current assignee: Shanghai Chenyang Biotechnology Co ltd
Priority date: 2021-12-24
Filing date: 2021-12-24
Publication date: 2022-04-12

Abstract

The invention relates to a supermolecule preparation with antioxidant, anti-sugar and anti-blue light effects and a preparation method thereof, and the supermolecule preparation is characterized by comprising the following components in percentage by mass: 0.5-5% of superoxide dismutase SOD, 0.08-5% of ergothioneine, 5-15% of tetrahydro-methyl pyrimidine carboxylic acid, 5-10% of decarboxylic carnosine, 0.006-0.2% of mevalonolactone, 5-60% of hydroxypropyl beta-cyclodextrin and 10-80% of trehalose. The supermolecule preparation with the effects of resisting oxygen, resisting sugar and resisting blue light, which is disclosed by the invention, has the advantages of good stability and good effects of resisting sugar, resisting oxygen and resisting blue light.

Description

Supermolecule preparation with antioxidant, anti-sugar and anti-blue-light effects and preparation method thereof

Technical Field

The invention relates to the field of cosmetics, and in particular relates to a supermolecule preparation with antioxidant, anti-sugar and anti-blue-light effects and a preparation method thereof.

Background

With the development of modern society, environmental pollution, electronic products and fast-paced life become more and more common. Endogenous metabolism in human bodies, ultraviolet irradiation and many other environmental pollutants can induce skin cells to generate free radicals and ROS, and excessive free radicals or ROS can damage cell components such as cell walls, lipid membranes, mitochondria and DNA, so that the skin is dull, aged and the like.

The food and environment can affect the glycosylation reaction of the skin, and the generated AGEs can be directly deposited on skin tissues to cause the skin to generate pigmentation, and can also activate a melanin secretion related signal pathway through the interaction with RAGE, increase the expression of related transcription factors and the activity of tyrosinase, promote the generation of melanin in melanocytes, and indirectly cause the skin pigmentation. The interaction between AGEs and RAGE also leads to a diminished antioxidant defense, a reduced antioxidant capacity of the body and inflammation.

Blue LIGHT, also known as HIGH ENERGY visible LIGHT, is an important component of sunlight, and is commonly found in various LED lamps, electronic screens, etc. The wavelength of the blue light is between 400nm and 480nm, is in the wave band with the highest energy in the visible light, and has higher energy. Therefore, the blue light can reach the deeper layer of the skin directly, has strong penetrating power, has lasting effect on the skin, is mainly manifested as melanin pigmentation, enables the skin to become loose, loses elasticity and accelerates the aging speed of the skin.

Superoxide Dismutase (SOD) can specifically remove Superoxide Dismutase (Superoxide Dismutase) produced in vivo, is a scavenger of endogenous oxygen, can regulate oxygen metabolism in vivo, has ultraviolet absorption function, and has effects of relieving mottle, removing wrinkle, and resisting aging. However, SOD has the disadvantages of instability, easy inactivation, etc., and the activity of SOD is related to the changes of amino acid residue, metal ions, coordination environment and imidazole bridge of the active center.

Ergothioneine is a natural antioxidant from common mushrooms in the nature, has various biological functions, such as physiological functions of antioxidation, anti-inflammation, ultraviolet radiation resistance, color protection and the like, and is also an important cell physiological protective agent. Through its powerful free radical scavenging ability, it exerts its effects through various mechanisms, and also activates antioxidant enzymes, such as SOD, which can scavenge reactive oxygen Radicals (ROS), thereby exerting its antioxidant effect. However, ergothioneine has the disadvantages of instability in solution, easy generation of sulfur odor after long-term storage, and the like.

The mevalonolactone exists in nature, participates in a plurality of biosynthesis processes, can self-regulate the output according to the self-demand of human body, improve the synthesis amount of coenzyme Q10, cholesterol and derivatives thereof in vivo, promote the proliferation of dermal fiber buds, improve the generation of epidermal basal layer collagen and improve wrinkles. However, the mevalonolactone is unstable and is easily hydrolyzed in aqueous solution, and the aqueous solution is acidic.

Supramolecules refer to highly complex and regular organized bodies formed by spontaneous assembly of two or more molecules of compounds through intermolecular non-covalent interactions. Intermolecular non-covalent bond effects mainly include hydrogen bonds, electrostatic effects, stacking effects, hydrophobic/hydrophilic effects, coordination bonds and the like, intermolecular interaction forces can be superposed to a certain extent, and a strong binding force can be obtained by a synergistic effect.

Disclosure of Invention

The invention aims to solve the technical problem of providing a supramolecular preparation with antioxidant, anti-sugar and anti-blue light effects and a preparation method thereof.

In order to solve the technical problems, the invention adopts the following technical scheme: a supramolecular preparation with antioxidant, anti-sugar and anti-blue light effects is characterized by comprising the following raw material components in percentage by mass: 0.5-5% of superoxide dismutase SOD, 0.08-5% of ergothioneine, 5-15% of tetrahydro-methyl pyrimidine carboxylic acid, 5-10% of decarboxylic carnosine, 0.006-0.2% of mevalonolactone, 5-60% of hydroxypropyl beta-cyclodextrin and 10-80% of trehalose.

The invention relates to a preparation method of a supramolecular preparation with antioxidant, anti-sugar and anti-blue light effects, which comprises the following steps:

A. weighing 0.5-5% of superoxide dismutase SOD, 0.08-5% of ergothioneine, 5-15% of tetrahydro-methylpyrimidine carboxylic acid, 5-10% of decarboxylic carnosine, 0.006-0.2% of mevalonolactone, 5-60% of hydroxypropyl beta-cyclodextrin and 10-80% of trehalose according to the proportion;

B. adding the obtained mixture into a reaction kettle of a supercritical device, pumping into CO2 by a CO2 high-pressure pump, adjusting the temperature in the reaction kettle of the supercritical device to 30-50 ℃, setting the pressure to be 6-20 Mpa, incubating and dissolving for 18-24 h in the supercritical state, then releasing CO2 by pressure relief, and cooling to room temperature to obtain the supermolecule preparation with the effects of resisting oxygen, sugar and blue light.

The supercritical equipment adopted by the invention is commercially available from HT350-100LX3, which is produced by Guizhou aerospace Wujiang electromechanical equipment, Limited liability company, the equipment pressure is less than 35MPa, the equipment temperature is less than 90 ℃, the rated flow is as follows: 1000L/H.

The CAS number of the superoxide dismutase is 9054-89-1, the activity is more than or equal to 25000U/g, and the central ion is Mn;

the ergothioneine CAS number: 497-30-3, purity not less than 98%;

the tetrahydromethylpyrimidine carboxylic acid is commercially available as RonaCare Ectoin from MERCK corporation;

the decarboxylated carnosine CAS no: 57022-38-5, the required content is more than or equal to 95%, the manufacturer has Shanghai Michelin Biotechnology limited;

the mevalonic lactone CAS number: 19115-49-2, the required content is not less than 90%, and the manufacturer has MERCK corporation;

the hydroxypropyl beta-cyclodextrin CAS number: 128446-35-5, manufactured by Shandong Binzhou Zhiyuan Biotech Co.

The invention relates to a supermolecule preparation with antioxidant, anti-sugar and anti-blue light effects, which is characterized in that firstly, under the excellent dissolving capacity of supercritical carbon dioxide, superoxide dismutase (SOD), ergothioneine, tetrahydro-methylpyrimidine carboxylic acid, decarboxylated carnosine, mevalonic lactone, hydroxypropyl beta-cyclodextrin and trehalose are dissolved to form a supercritical cosolvent, at the moment, an amino acid chain of the SOD can have covalent bond with an alanine chain of the decarboxylated carnosine, the central Mn of the SOD and the carboxyl of the tetrahydro-methylpyrimidine carboxylic acid have electrostatic interaction, and the N of the ergothioneine⁺Can have electrostatic interaction with hydroxyl of mevalonolactone, pi-pi accumulation exists between cyclic structure of tetrahydro-methylpyrimidine carboxylic acid and cyclic structure of decarboxylated carnosine and ergothioneine, and in addition, ergothioneine,The tetrahydro-methyl pyrimidine carboxylic acid, the decarboxylated carnosine and the mevalonic lactone all have a hydrophobic annular structure and can be embedded in an inner cavity of the hydroxypropyl beta-cyclodextrin, a plurality of hydroxyl groups of trehalose are combined with the hydrophilic outer wall of the hydroxypropyl beta-cyclodextrin, a plurality of acting forces are combined to form a firm supramolecular structure, and then carbon dioxide is removed, so that the supramolecular preparation containing superoxide dismutase (SOD), ergothioneine, tetrahydro-methyl pyrimidine carboxylic acid, the decarboxylated carnosine, the mevalonolactone, trehalose and hydroxypropyl beta-cyclodextrin is obtained.

The invention has the beneficial effects that: 1) the stability is good, and the problems of SOD volatility, ergothioneine odor and instability of the mevalonic lactone can be solved simultaneously; 2) has good effects of resisting sugar, resisting oxygen and resisting blue light.

To demonstrate the advantageous effects of the present invention, the following experiment was further performed.

The test for verifying the benefit of the supramolecular preparation with the effects of resisting oxygen, sugar and blue light comprises the following steps:

(1) confirmation of supramolecular structure:

confirming the formation of supramolecules by differential scanning calorimetry, raising the temperature at a speed of 10 ℃/min, scanning at a temperature range of 10-300 ℃, and obtaining a differential scanning calorimetry output curve graph of a sample A, a sample B, a sample C, a sample D, a sample E and a sample F shown in figure 1, wherein A is the sample A, B is the sample B, C is the sample C, D is the sample D, E is the sample E, and F is the sample F; wherein the sample A is ergothioneine, the sample B is tetrahydro-methylpyrimidine carboxylic acid, the sample C is mevalonolactone, the sample D is decarbocarnosine, the sample E is trehalose, and the sample F is the supramolecular preparation with the effects of resisting oxygen, sugar and blue light obtained in example 4.

As can be seen from FIG. 1, sample A has a characteristic peak at around 250 ℃, which is related to the melting temperature of ergothioneine; sample B has a characteristic peak at around 280 ℃, which is related to the melting temperature of the tetrahydro-methyl-pyrimidine-carboxylic acid; sample C has a characteristic peak near 28 ℃, which is related to the melting temperature of the mevalonolactone; sample D has a characteristic peak around 158 ℃, which is related to the melting temperature of the decarboxylated carnosine; sample E has a characteristic peak near 214 ℃, which is related to the melting temperature of trehalose; and the curve of the sample F subjected to the supermolecule treatment is smooth, and the characteristic peaks of ergothioneine, tetrahydromethylpyrimidine carboxylic acid, mevalonic lactone, decarboxylated carnosine and trehalose disappear, so that a new stable structure is formed with hydroxypropyl beta-cyclodextrin and superoxide dismutase SOD, and the formation of the supermolecule structure can be represented.

(2) Stability test:

supramolecular formulations or active compound combinations were prepared according to the active substance percentages indicated in table 1, examples 1-6 being supramolecular formulations prepared according to the present technique for different active content ratios.

Comparative example 1 was a composition consistent with that described in example 3, but without the use of supercritical technology, mixed with simple stirring.

Comparative example 2 was prepared as described in example 3, but without the addition of superoxide dismutase SOD and with a composition identical to that described in example 3.

Comparative example 3 was prepared as described in example 3, but without the addition of ergothioneine, and the other compositions were identical to those described in example 3.

Comparative example 4 was prepared as described in example 3, but without the addition of mevalonolactone and with the other compositions consistent with those described in example 3.

Table 1 active substance mass percentages and methods of preparation in the examples and comparative examples

The 5% aqueous solution (mass percentage) of the above samples was placed at 5 ℃, 45 ℃ and under light conditions for stability test, and the samples were tested for appearance, odor and pH change at 2 weeks, 4 weeks, 8 weeks and 12 weeks, respectively, and the stability test was as shown in table 2 below.

TABLE 2 stability test results

O means no discoloration, no odor, no precipitation, no delamination, no significant change in pH (within. + -. 0.5).

From the test condition, the supermolecule preparation prepared by the invention has better illumination, high and low temperature and PH stability.

(3) Irritation test

60 volunteers aged 18-60 were selected and subjected to irritation test according to the cosmetic safety technical Specification 2015 edition-human skin patch test.

The selected examples 1-6 and comparative examples 1-4 are respectively added into the face cream according to the mass percent of 2%, and the formula composition of the face cream is shown in the table 3. Adding the sample into a spot tester, then applying the spot tester with the sample on the back of a subject, removing the spot tester of the subject after 24h, and observing skin reaction at 0.5h, 24h and 48h after removing the spot tester. And recording reaction results according to a grading standard, wherein the reaction results are used as a standard for evaluating the irritation, wherein 0 grade is negative reaction, 1 grade is suspicious reaction, 2 grade is weak positive reaction, 3 grade is strong positive reaction, 4 grade is extremely strong positive reaction, and the higher the score is, the stronger the irritation is.

TABLE 3 irritation test sample cream composition

TABLE 4 results of irritation test

As can be seen from Table 4, compared with some adverse reactions in the comparative examples, the supramolecular preparation prepared by the invention has no adverse reaction on human skin, which indicates that the supramolecular preparation prepared by the supercritical technology has the characteristic of milder reaction.

(4) Efficacy test

The examples and controls listed in table 1 above were tested for efficacy, including antioxidant, anti-glycation and anti-blue effects and human efficacy, as follows:

A. antioxidation: the ABTS free radical removal detection method comprises the steps of weighing 5mg of sample (the supramolecular preparation in the embodiment and the active compound in the comparative example) and adding the sample into 5 mL of deionized water, carrying out ultrasonic treatment for 10 min, and centrifuging the sample liquid for 10 min at the rotating speed of 8000 r/min. 3 mL of sample supernatant was removed and diluted to a concentration of 1-0.0156 mg/mL by a sesquidilution method. Adding 0.2 mL of each sample solution into 4 mL of ABTS solution (7 mmol/L), mixing, shaking to make it uniform, standing for reaction for 30 min, and keeping at room temperature and in dark. The absorbance was then measured at a wavelength of 734 nm. After 3 repeated experiments, the average value was obtained. The free radical clearance rate is calculated by the formula (A) ABTS free radical clearance rate ═ A₀-A）/A₀*100

A₀Represents the absorbance at 734 nm of 4 mL of a 7 mmol/L ABTS solution plus 0.2 mL of deionized water;

a represents the absorbance at 734 nm of 4 mL of a 7 mmol/L ABTS solution plus 0.2 mL of sample.

TABLE 5 results of antioxidant experiments

The antioxidant results are shown in table 5, where the examples of the patent have a significant effect on the antioxidant effect, up to 98.6%, while the comparative examples have a significantly lower antioxidant effect without the use of the technology of the patent and lack of a certain active ingredient.

B. Anti-saccharification: inhibition of non-enzymatic glycosylation assay: the experiment simulates the non-enzymatic glycosylation reaction in human body by mixed culture of collagen and glucosone, the inhibitor of the non-enzymatic glycosylation reaction is added to inhibit the reaction, the fluorescence intensity of the system with the inhibitor is weaker than that of the control without the inhibitor, and the inhibition rate is used for reflecting the inhibition of the sample on the non-enzymatic glycosylation reaction. A certain amount of a mixed solution containing 10mg/mL of collagen and 0.25mol/L of glucosone was taken as a reaction solution. PBS is prepared into solutions with the mass fraction of 1 percent in examples 1-6 and comparative examples 1-4 to serve as treatment groups, a positive control group, a negative control group (PBS replaces a sample) and a blank group (PBS replaces a reaction solution) are arranged, each group is provided with 3 parallel tubes, the samples are cultured in a dark place at 37 ℃, the fluorescence intensity of each test group is detected by a fluorescence microplate reader at 370nm (absorption wavelength)/440 nm (excitation wavelength), and the inhibition rate is calculated.

TABLE 6 results of anti-glycation experiments

As shown in Table 6, the anti-glycation test results of the examples in this patent showed that the anti-glycation effect was superior, whereas the anti-glycation effect of the active compound of comparative example 1, which was not subjected to the supercritical technology of this patent and was simply stirred, was weak, and the anti-glycation effect of the supramolecular preparation prepared in comparative examples 2 to 4, which was prepared in the absence of an active ingredient, was significantly reduced as compared to the examples.

C. Resisting blue light: human epidermal melanocytes are placed in a six-well plate, then the supermolecule preparation and the active compound of the above embodiment and comparative example are added for culturing for 48h, blue light is used for irradiation, then the melanin is extracted by using an alkaline extract, the melanin is quantitatively detected at the position of 405 nm of wavelength by an enzyme-labeling instrument, and meanwhile, the inhibition rate of the added supermolecule preparation and the active compound on the increase of the melanin in the cells induced by the blue light is calculated by comparing with a blank group (without adding any substance).

TABLE 7 anti-blue light test results

The blue light resisting experiment results are shown in table 7, the inhibition rate of examples 1-6 on increase of blue light induced melanin in cells is high, and the blue light resisting effect is good in the examples in the patent, and in the comparative examples, compared with the examples, the blue light resisting effect is remarkably reduced in the supermolecule preparation which is prepared by using no active compound or lack of a certain active ingredient and is simply stirred by using the supercritical technology in the patent.

D. Human body efficacy experiment

In order to further prove the efficacy of the supramolecular preparation prepared by the invention, a human body efficacy test is carried out, and the specific steps are as follows: 30 female volunteers of 40-55 years old, with the wrinkle grade of the left and right canthus consistent and larger than 1, who are not prepared for pregnancy and are in lactation and pregnancy are selected and randomly divided into an experimental group and a control group, the facial cream of the embodiment 4 is added to the left and right cheeks in proportion once every 28 days, the facial cream without the supermolecule preparation is used in the control group (the using amount is kept consistent and is about 0.5 g/time), and the experiment requires that the volunteers mainly move indoors during the test period, so that the long-time unprotected exposure to the sunlight is avoided. The eye angle of the volunteer was selected as the test site, and the test was performed before use and after the volunteer cleaned the face after 28 days, and the number of wrinkles, the ratio of the average depth of total wrinkles to the area of wrinkles, were measured by the same person using the PROMIS LITE wrinkle meter of LMI Technologies, germany, and the skin gloss value was measured using the skin tester glossmeter GL 200, and the results of the human efficacy test are shown in table 8.

Table 8 human body efficacy test results

As can be seen from table 8, the number of wrinkles in the test group was reduced by 20.32%, the average ratio of the area of wrinkles was reduced by 20.42%, and the average depth of total wrinkles was reduced by 20.49% in the follow-up before and after use. While higher measured values for skin gloss indicate better skin gloss, the experimental group showed a 27.32% improvement in skin gloss after using the cream prepared in example 4, as shown in table 8. Therefore, the antioxidant, anti-sugar and anti-blue light supramolecular preparation prepared by the invention has better anti-wrinkle and brightening effects.

Drawings

FIG. 1 is a graph of the differential scanning calorimetry output of sample A, B, C, D, E, F.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below.

Example 1: a preparation method of a supramolecular preparation with antioxidant, anti-sugar and anti-blue light effects is characterized by comprising the following steps:

A. weighing 0.5% of superoxide dismutase SOD, 0.08% of ergothioneine, 5% of tetrahydro-methylpyrimidine carboxylic acid, 5% of decarboxylated carnosine, 0.006% of mevalonic lactone, 60% of hydroxypropyl beta-cyclodextrin and 29.414% of trehalose according to the proportion;

B. adding the obtained mixture into a reaction kettle of a supercritical device, pumping into CO2 by a CO2 high-pressure pump, adjusting the temperature in the reaction kettle of the supercritical device to 30 ℃, setting the pressure to be 20Mpa, incubating and dissolving for 24h under the supercritical state, then releasing CO2 by pressure relief, and cooling to room temperature to obtain the supermolecule preparation with the effects of resisting oxygen, sugar and blue light.

Example 2: a preparation method of a supramolecular preparation with antioxidant, anti-sugar and anti-blue light effects is characterized by comprising the following steps:

A. weighing 1% of superoxide dismutase SOD, 1% of ergothioneine, 7% of tetrahydro-methylpyrimidine carboxylic acid, 6% of decarboxylated carnosine, 0.01% of mevalonolactone, 40% of hydroxypropyl beta-cyclodextrin and 44.99% of trehalose according to the proportion;

B. adding the obtained mixture into a reaction kettle of a supercritical device, pumping into CO2 by a CO2 high-pressure pump, adjusting the temperature in the reaction kettle of the supercritical device to 35 ℃, setting the pressure to 18Mpa, incubating and dissolving for 22h under the supercritical state, then releasing CO2 by pressure relief, and cooling to room temperature to obtain the supermolecule preparation with the effects of resisting oxygen, sugar and blue light.

Example 3: a preparation method of a supramolecular preparation with antioxidant, anti-sugar and anti-blue light effects is characterized by comprising the following steps:

A. weighing 2% of superoxide dismutase SOD, 2% of ergothioneine, 9% of tetrahydro-methylpyrimidine carboxylic acid, 7% of decarboxylated carnosine, 0.05% of mevalonolactone, 30% of hydroxypropyl beta-cyclodextrin and 49.95% of trehalose according to the proportion;

B. adding the obtained mixture into a reaction kettle of a supercritical device, pumping into CO2 by a CO2 high-pressure pump, adjusting the temperature in the reaction kettle of the supercritical device to 38 ℃, setting the pressure to be 15Mpa, incubating and dissolving for 21h under the supercritical state, then releasing pressure to release CO2, and cooling to room temperature to obtain the supermolecule preparation with antioxidant, anti-sugar and anti-blue light effects.

Example 4: a preparation method of a supramolecular preparation with antioxidant, anti-sugar and anti-blue light effects is characterized by comprising the following steps:

A. weighing 3% of superoxide dismutase SOD, 3% of ergothioneine, 11% of tetrahydro-methylpyrimidine carboxylic acid, 8% of decarboxylated carnosine, 0.10% of mevalonolactone, 20% of hydroxypropyl beta-cyclodextrin and 54.9% of trehalose according to the proportion;

B. adding the obtained mixture into a reaction kettle of a supercritical device, pumping into CO2 by a CO2 high-pressure pump, adjusting the temperature in the reaction kettle of the supercritical device to 40 ℃, setting the pressure to 12Mpa, incubating and dissolving for 20h under the supercritical state, then releasing CO2 by pressure relief, and cooling to room temperature to obtain the supermolecule preparation with the effects of resisting oxygen, sugar and blue light.

Example 5: a preparation method of a supramolecular preparation with antioxidant, anti-sugar and anti-blue light effects is characterized by comprising the following steps:

A. weighing 4% of superoxide dismutase SOD, 4% of ergothioneine, 13% of tetrahydro-methylpyrimidine carboxylic acid, 9% of decarboxylated carnosine, 0.15% of mevalonolactone, 10% of hydroxypropyl beta-cyclodextrin and 59.85% of trehalose according to the proportion;

B. adding the obtained mixture into a reaction kettle of a supercritical device, pumping into CO2 by a CO2 high-pressure pump, adjusting the temperature in the reaction kettle of the supercritical device to 45 ℃, setting the pressure to be 9Mpa, incubating and dissolving for 19 hours in the supercritical state, then releasing pressure to release CO2, and cooling to room temperature to obtain the supermolecule preparation with antioxidant, anti-sugar and anti-blue light effects.

Example 6: a preparation method of a supramolecular preparation with antioxidant, anti-sugar and anti-blue light effects is characterized by comprising the following steps:

A. weighing 5% of superoxide dismutase SOD, 5% of ergothioneine, 15% of tetrahydro-methylpyrimidine carboxylic acid, 10% of decarboxylated carnosine, 0.2% of mevalonolactone, 5% of hydroxypropyl beta-cyclodextrin and 59.8% of trehalose according to the proportion;

B. adding the obtained mixture into a reaction kettle of a supercritical device, pumping into CO2 by a CO2 high-pressure pump, adjusting the temperature in the reaction kettle of the supercritical device to 50 ℃, setting the pressure to be 6Mpa, incubating and dissolving for 18h under the supercritical state, then releasing pressure to release CO2, and cooling to room temperature to obtain the supermolecule preparation with antioxidant, anti-sugar and anti-blue light effects.

Claims

1. A supramolecular preparation with antioxidant, anti-sugar and anti-blue light effects is characterized in that: the composite material comprises the following components in percentage by mass: 0.5-5% of superoxide dismutase SOD, 0.08-5% of ergothioneine, 5-15% of tetrahydro-methyl pyrimidine carboxylic acid, 5-10% of decarboxylic carnosine, 0.006-0.2% of mevalonolactone, 5-60% of hydroxypropyl beta-cyclodextrin and 10-80% of trehalose.

2. The process for preparing supramolecular preparation with antioxidant, antidiabetic and anti-blue light effects as claimed in claim 1, characterized by the following steps: a. Weighing 0.5-5% of superoxide dismutase SOD, 0.08-5% of ergothioneine, 5-15% of tetrahydro-methylpyrimidine carboxylic acid, 5-10% of decarboxylic carnosine, 0.006-0.2% of mevalonolactone, 5-60% of hydroxypropyl beta-cyclodextrin and 10-80% of trehalose according to the proportion;