CN114304429A - Traditional Chinese medicine composite probiotic feed additive for broiler chickens and preparation method thereof - Google Patents
Traditional Chinese medicine composite probiotic feed additive for broiler chickens and preparation method thereof Download PDFInfo
- Publication number
- CN114304429A CN114304429A CN202210051213.1A CN202210051213A CN114304429A CN 114304429 A CN114304429 A CN 114304429A CN 202210051213 A CN202210051213 A CN 202210051213A CN 114304429 A CN114304429 A CN 114304429A
- Authority
- CN
- China
- Prior art keywords
- composite probiotic
- traditional chinese
- feed additive
- powder
- broiler chickens
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000287828 Gallus gallus Species 0.000 title claims abstract description 48
- 239000006041 probiotic Substances 0.000 title claims abstract description 38
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 38
- 239000002131 composite material Substances 0.000 title claims abstract description 36
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 36
- 239000003814 drug Substances 0.000 title claims abstract description 24
- 239000003674 animal food additive Substances 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 239000000843 powder Substances 0.000 claims abstract description 39
- 241000194103 Bacillus pumilus Species 0.000 claims abstract description 28
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 28
- 241000186660 Lactobacillus Species 0.000 claims abstract description 28
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 28
- 241000411851 herbal medicine Species 0.000 claims abstract description 24
- 235000013330 chicken meat Nutrition 0.000 claims abstract description 17
- 239000011812 mixed powder Substances 0.000 claims abstract description 17
- 244000035851 Chrysanthemum leucanthemum Species 0.000 claims abstract description 13
- 235000008495 Chrysanthemum leucanthemum Nutrition 0.000 claims abstract description 13
- 240000000691 Houttuynia cordata Species 0.000 claims abstract description 13
- 244000179560 Prunella vulgaris Species 0.000 claims abstract description 13
- 235000008113 selfheal Nutrition 0.000 claims abstract description 13
- 241000334160 Isatis Species 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims description 27
- 229920001817 Agar Polymers 0.000 claims description 20
- 239000001888 Peptone Substances 0.000 claims description 20
- 108010080698 Peptones Proteins 0.000 claims description 20
- 239000008272 agar Substances 0.000 claims description 20
- 235000019319 peptone Nutrition 0.000 claims description 20
- 239000001963 growth medium Substances 0.000 claims description 18
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 17
- 230000001580 bacterial effect Effects 0.000 claims description 17
- 239000008103 glucose Substances 0.000 claims description 17
- 244000118350 Andrographis paniculata Species 0.000 claims description 12
- 241000045403 Astragalus propinquus Species 0.000 claims description 12
- 235000013719 Houttuynia cordata Nutrition 0.000 claims description 12
- 235000006533 astragalus Nutrition 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 12
- 240000004980 Rheum officinale Species 0.000 claims description 11
- 235000008081 Rheum officinale Nutrition 0.000 claims description 11
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 238000009835 boiling Methods 0.000 claims description 8
- 239000000725 suspension Substances 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 7
- 239000000706 filtrate Substances 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 5
- 239000010231 banlangen Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 241000628997 Flos Species 0.000 claims description 3
- 239000009636 Huang Qi Substances 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 claims description 3
- 238000002844 melting Methods 0.000 claims description 3
- 230000008018 melting Effects 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 3
- UAJTZZNRJCKXJN-UHFFFAOYSA-M sodium;2-dodecoxy-2-oxoethanesulfonate Chemical compound [Na+].CCCCCCCCCCCCOC(=O)CS([O-])(=O)=O UAJTZZNRJCKXJN-UHFFFAOYSA-M 0.000 claims description 3
- 238000001694 spray drying Methods 0.000 claims description 3
- 239000008223 sterile water Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 239000002699 waste material Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000000889 atomisation Methods 0.000 claims description 2
- 238000004806 packaging method and process Methods 0.000 claims description 2
- 238000011085 pressure filtration Methods 0.000 claims description 2
- 241000191940 Staphylococcus Species 0.000 abstract description 9
- 230000002401 inhibitory effect Effects 0.000 abstract description 9
- 241000194017 Streptococcus Species 0.000 abstract description 7
- 201000010099 disease Diseases 0.000 abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 7
- 230000036039 immunity Effects 0.000 abstract description 7
- 241000588724 Escherichia coli Species 0.000 abstract description 6
- 241000607142 Salmonella Species 0.000 abstract description 6
- 241000712461 unidentified influenza virus Species 0.000 abstract description 6
- 241000607768 Shigella Species 0.000 abstract description 5
- 238000009395 breeding Methods 0.000 abstract description 5
- 230000001488 breeding effect Effects 0.000 abstract description 5
- 230000000968 intestinal effect Effects 0.000 abstract description 5
- 208000035240 Disease Resistance Diseases 0.000 abstract description 4
- 206010012735 Diarrhoea Diseases 0.000 abstract description 3
- 244000144972 livestock Species 0.000 abstract description 3
- 241000202726 Bupleurum Species 0.000 abstract description 2
- 235000009411 Rheum rhabarbarum Nutrition 0.000 abstract description 2
- 229940126680 traditional chinese medicines Drugs 0.000 abstract description 2
- 241000746375 Andrographis Species 0.000 abstract 1
- 241000758794 Asarum Species 0.000 abstract 1
- 241000555682 Forsythia x intermedia Species 0.000 abstract 1
- 235000013717 Houttuynia Nutrition 0.000 abstract 1
- 244000299790 Rheum rhabarbarum Species 0.000 abstract 1
- 229940107666 astragalus root Drugs 0.000 abstract 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 6
- 230000001717 pathogenic effect Effects 0.000 description 4
- 238000010926 purge Methods 0.000 description 4
- 241000588769 Proteus <enterobacteria> Species 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000003825 pressing Methods 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 230000008961 swelling Effects 0.000 description 3
- 231100000331 toxic Toxicity 0.000 description 3
- 230000002588 toxic effect Effects 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 208000004880 Polyuria Diseases 0.000 description 2
- 241000607764 Shigella dysenteriae Species 0.000 description 2
- 241000193998 Streptococcus pneumoniae Species 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000035619 diuresis Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 206010022000 influenza Diseases 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000027939 micturition Effects 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 235000013594 poultry meat Nutrition 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- 241000193468 Clostridium perfringens Species 0.000 description 1
- 241000186216 Corynebacterium Species 0.000 description 1
- 241000186227 Corynebacterium diphtheriae Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 208000004232 Enteritis Diseases 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010068319 Oropharyngeal pain Diseases 0.000 description 1
- 201000007100 Pharyngitis Diseases 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 241000219061 Rheum Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000006030 antibiotic growth promoter Substances 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 230000005784 autoimmunity Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 206010013023 diphtheria Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 229940007046 shigella dysenteriae Drugs 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention belongs to the technical field of livestock raising, in particular to a traditional Chinese medicine composite probiotic feed additive for broiler chickens and a preparation method thereof, aiming at the technical problems of low immunity, poor disease resistance and easy occurrence of diseases of the broiler chickens brought forward by the background technology, the following scheme is provided, wherein the traditional Chinese medicine composite probiotic feed additive comprises a Chinese herbal medicine mixed powder and a composite probiotic powder, and the Chinese herbal medicine mixed powder comprises the following components in percentage: 5 to 10 percent of astragalus root, 3 to 5 percent of rhubarb, 5 to 10 percent of forsythia, 10 to 20 percent of isatis root, 1 to 5 percent of common andrographis herb, 10 to 15 percent of bupleurum, 1 to 5 percent of selfheal, 5 to 20 percent of wild chrysanthemum flower and 35 to 40 percent of heartleaf houttuynia herb. The feed additive adopts various traditional Chinese medicines, has good inhibiting effect on streptococcus, staphylococcus, escherichia coli, salmonella, shigella, chicken influenza virus and the like, enhances the immunity of broilers, and can improve intestinal probiotics of the broilers, reduce the diarrhea probability of the broilers and reduce the breeding loss by being mixed with lactobacillus, bifidobacterium and bacillus pumilus to be mixed into the feed.
Description
Technical Field
The invention relates to the technical field of livestock raising, in particular to a traditional Chinese medicine composite probiotic feed additive for broiler chickens and a preparation method thereof.
Background
In the process of livestock and poultry breeding, particularly in the process of breeding in a chicken farm, the amount of broiler motion is low, the immunity of chicken flocks is weak, particularly in autumn and winter, viral diseases are frequent, viral influenza is a common disease in each chicken farm, broiler diseases are easy to occur in the chicken farm, and if treatment measures are not timely taken, the death of chicks is easy to cause, the fat loss of adult chicks is reduced, the weight gain is reduced, the time of marketing is delayed, the disease resistance of the broiler is reduced, and huge economic loss is caused to farmers.
Pathogenic intestinal bacteria in broiler chickens can cause diarrhea, infection, abnormal liver function, and reduce digestion and absorption of nutrients. Beneficial bacteria inhibit the growth of various pathogenic bacteria through different mechanisms, stimulate the immune system, synthesize vitamins and the like. For many years, it has proven beneficial to use feed antibiotic additives which stabilize the intestinal flora, improve the growth performance of poultry and prevent certain specific intestinal pathologies, such as necrotic enteritis caused by clostridium perfringens. However, antibiotics eventually cause harmful bacteria in human bodies to become resistant to antibiotics after being used on animals, and currently, antibiotic growth promoters have been banned or limited in use in the breeding industry in many countries.
The traditional Chinese medicine is a treasure of Chinese culture, is inherited for thousands of years, treats both principal and secondary aspects of diseases, has high safety, no medicine residue and wide application, is used in combination with vaccines and western veterinary medicines, can effectively control and prevent the invasion of various bacteria and viruses to chickens, fully exerts the synergistic effect, promotes the improvement of the autoimmunity of the chickens, and thus plays an excellent role in preventing and treating diseases.
Disclosure of Invention
Based on the technical problems of low immunity, poor disease resistance and easy occurrence of diseases of the broiler chickens, the invention provides a traditional Chinese medicine composite probiotic feed additive for the broiler chickens and a preparation method thereof.
The invention provides a traditional Chinese medicine composite probiotic feed additive for broiler chickens, which comprises Chinese herbal medicine mixed powder and composite probiotic powder, wherein the Chinese herbal medicine mixed powder comprises the following components in percentage: 5-10% of astragalus membranaceus, 3-5% of rheum officinale, 5-10% of fructus forsythiae, 10-20% of isatis root, 1-5% of andrographis paniculata, 10-15% of radix bupleuri, 1-5% of selfheal, 5-20% of wild chrysanthemum flower and 35-40% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: 25-35% of peptone, 30-40% of agar, 10-25% of glucose, 3-5% of lactobacillus, 5-10% of bifidobacterium and 5-10% of bacillus pumilus.
Preferably, the Chinese herbal medicine mixed powder comprises the following components in percentage: 10% of astragalus membranaceus, 5% of rheum officinale, 10% of fructus forsythiae, 10% of isatis root, 2% of andrographis paniculata, 10% of radix bupleuri, 3% of selfheal, 15% of wild chrysanthemum flower and 35% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: peptone 25%, agar 30%, glucose 25%, lactobacillus 3%, bifidobacterium 8% and bacillus pumilus 9%.
Preferably, the Chinese herbal medicine mixed powder comprises the following components in percentage: 5% of astragalus membranaceus, 3% of rheum officinale, 10% of fructus forsythiae, 20% of isatis root, 4% of andrographis paniculata, 10% of radix bupleuri, 1% of selfheal, 10% of wild chrysanthemum flower and 37% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: 35% of peptone, 25% of agar, 20% of glucose, 4% of lactobacillus, 8% of bifidobacterium and 8% of bacillus pumilus.
Preferably, the Chinese herbal medicine mixed powder comprises the following components in percentage: 8% of astragalus membranaceus, 4% of rheum officinale, 8% of fructus forsythiae, 15% of isatis root, 5% of andrographis paniculata, 10% of radix bupleuri, 5% of selfheal, 20% of wild chrysanthemum flower and 25% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: 30% of peptone, 35% of agar, 15% of glucose, 5% of lactobacillus, 7% of bifidobacterium and 8% of bacillus pumilus.
The invention also provides a preparation method of the traditional Chinese medicine composite probiotic feed additive for broiler chickens, which comprises the following steps:
s1: respectively dissolving lactobacillus, bifidobacterium and bacillus pumilus samples collected from chicken intestinal tracts in sterilized sterile water, shaking at the temperature of between 30 and 37 ℃ at the speed of between 150 and 300r/min for 30 to 60min, dispersing flora, and respectively preparing bacterial liquids of the lactobacillus, the bifidobacterium and the bacillus pumilus;
s2: taking peptone, glucose and agar according to mass percent, crushing the peptone and the agar, boiling, melting and mixing the peptone, the glucose and the agar, adjusting the pH value to 7.0 by using a sodium hydroxide solution, naturally cooling and sterilizing to obtain a culture medium;
s3: respectively taking liquid of lactobacillus, bifidobacterium and bacillus pumilus, dividing the prepared culture medium into three parts, crushing the culture medium, respectively placing the liquid of the lactobacillus, the liquid of the bifidobacterium and the liquid of the bacillus pumilus on the three parts of the culture medium to prepare three parts of bacterial suspension, placing the three parts of bacterial suspension into an aseptic environment, culturing for 48-72 h at 30-37 ℃, and respectively amplifying the three strains of the lactobacillus, the bifidobacterium and the bacillus pumilus;
s4: taking out the amplified strain, vibrating and crushing the strain by a centrifugal machine, and atomizing and drying the strain into powder at low temperature to obtain strain powder;
s5: pulverizing processed radix astragali, radix et rhizoma Rhei, fructus forsythiae, radix Isatidis, herba Andrographitis, bupleuri radix, Prunellae Spica, flos Chrysanthemi Indici and herba Houttuyniae to 100-200 mesh, mixing, boiling, centrifuging to extract Chinese herbal essence, and spray drying to obtain Chinese herbal essence powder;
s6: mixing the strain powder and the Chinese herbal medicine essence powder at a certain proportion, quantitatively packaging, and cold storing.
Preferably, the volume ratio of the bacterial liquid to the culture medium in the S3 is 3-5: 1, and the bacterial liquid and the crushed culture medium are shaken at the temperature of 30-37 ℃ at 200-300 r/min for 10-15 min.
Preferably, the residue and gauze filtered in S4 should be subjected to pressure filtration, and the pressure filtrate is collected and filtered, and the filtered pressure filtrate is combined with the filtrate, so as to avoid waste of bacteria liquid.
Preferably, in S5, the mixing time of the Chinese medicinal powder is 30min-60min, and the boiling time is 60min-120 min.
Preferably, the mixing mass ratio of the strain powder and the Chinese herbal medicine essence powder in the S6 is 1-1.5: 2.5-3.5.
The beneficial effects of the invention are as follows:
1. the effects of the Chinese herbal medicines are shown as follows;
the astragalus membranaceus has the effects of tonifying qi, strengthening the exterior, protecting the liver, promoting urination and enhancing the immune function of the chicken;
rhubarb: purgation, purging pathogenic fire, removing toxic substance, cooling blood, stopping bleeding, clearing away dampness and heat, and inhibiting viruses such as influenza, and has broad-spectrum antibacterial and antiviral effects, and can be used for preventing and treating streptococcus, staphylococcus, Diplococcus pneumoniae, Corynebacterium diphtheriae, Bacillus paratyphi, Bacillus dysenteriae, etc.;
fructus forsythiae: clearing away heat and toxic materials, relieving swelling, dispersing pathogen accumulation, dispelling pathogenic wind and heat, clearing heart fire, promoting urination, remarkably killing bacteria such as streptococcus, staphylococcus, pneumococcus, shigella, corynebacterium, escherichia coli, salmonella and proteus, and strongly inhibiting chicken influenza virus;
radix isatidis: clearing away heat and toxic materials, cooling blood, relieving sore throat, improving disease resistance of chicken, and inhibiting Bacillus, Streptococcus, Staphylococcus, Escherichia coli, Salmonella, Shigella, and chicken influenza virus;
andrographis paniculata: clearing heat and purging fire, promoting diuresis and relieving swelling, and has strong inhibiting effect on streptococcus, staphylococcus, salmonella, Escherichia coli, Pseudomonas aeruginosa, proteus, Diplococcus pneumoniae, etc.;
bupleurum root: relieving exterior syndrome, relieving fever, dispersing stagnated liver qi, relieving qi stagnation, regulating immunity, and resisting stress. Has strong inhibiting effect on chicken influenza virus;
selfheal: clearing heat and purging fire, resolving hard mass and detumescence, relieving cough and eliminating phlegm, relieving asthma and diminishing inflammation, and has strong antibacterial effect on staphylococcus, shigella, salmonella, pseudomonas, escherichia coli, tubercle bacillus, shigella dysenteriae, bacillus proteus, fungi and the like;
wild chrysanthemum flower: clearing heat and purging fire, removing toxic substances and relieving swelling, and has obvious inhibiting effect on staphylococcus, diphtheria bacillus, dysentery bacillus, chicken influenza virus, etc.;
houttuynia cordata: clearing away heat and toxic material, inducing diuresis, invigorating kidney, enhancing immunity, and resisting stress, and has inhibiting effect on Staphylococcus, Streptococcus, fungi and viruses.
In conclusion, the feed adopts various traditional Chinese medicines, has good inhibiting effect on streptococcus, staphylococcus, escherichia coli, salmonella, shigella, chicken influenza virus and the like, enhances the immunity of broiler chickens, and can improve intestinal probiotics of the broiler chickens, reduce the diarrhea probability of the broiler chickens and reduce the breeding loss by being mixed with lactobacillus, bifidobacterium and bacillus pumilus to be mixed into the feed.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.
The embodiment 1 discloses a traditional Chinese medicine composite probiotic feed additive for broiler chickens, which comprises a Chinese herbal medicine mixed powder and a composite probiotic powder, wherein the Chinese herbal medicine mixed powder comprises the following components in percentage: 10% of astragalus membranaceus, 5% of rheum officinale, 10% of fructus forsythiae, 10% of isatis root, 2% of andrographis paniculata, 10% of radix bupleuri, 3% of selfheal, 15% of wild chrysanthemum flower and 35% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: peptone 25%, agar 30%, glucose 25%, lactobacillus 3%, bifidobacterium 8% and bacillus pumilus 9%.
The embodiment 2 discloses a traditional Chinese medicine composite probiotic feed additive for broiler chickens, which comprises a Chinese herbal medicine mixed powder and a composite probiotic powder, wherein the Chinese herbal medicine mixed powder comprises the following components in percentage: 5% of astragalus membranaceus, 3% of rheum officinale, 10% of fructus forsythiae, 20% of isatis root, 4% of andrographis paniculata, 10% of radix bupleuri, 1% of selfheal, 10% of wild chrysanthemum flower and 37% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: 35% of peptone, 25% of agar, 20% of glucose, 4% of lactobacillus, 8% of bifidobacterium and 8% of bacillus pumilus.
Embodiment 3, the Chinese herbal medicine mixed powder comprises the following components in percentage: 8% of astragalus membranaceus, 4% of rheum officinale, 8% of fructus forsythiae, 15% of isatis root, 5% of andrographis paniculata, 10% of radix bupleuri, 5% of selfheal, 20% of wild chrysanthemum flower and 25% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: 30% of peptone, 35% of agar, 15% of glucose, 5% of lactobacillus, 7% of bifidobacterium and 8% of bacillus pumilus.
The preparation method comprises the following steps:
s1: respectively dissolving lactobacillus, bifidobacterium and bacillus pumilus samples collected from chicken intestinal tracts in sterilized sterile water, shaking at the temperature of between 30 and 37 ℃ at the speed of between 150 and 300r/min for 30 to 60min, dispersing flora, and respectively preparing bacterial liquids of the lactobacillus, the bifidobacterium and the bacillus pumilus;
s2: taking peptone, glucose and agar according to mass percent, crushing the peptone and the agar, boiling, melting and mixing the peptone, the glucose and the agar, adjusting the pH value to 7.0 by using a sodium hydroxide solution, naturally cooling and sterilizing to obtain a culture medium;
s3: respectively taking liquid of lactobacillus, bifidobacterium and bacillus pumilus, dividing the prepared culture medium into three parts, crushing the culture medium, respectively placing the liquid of the lactobacillus, the liquid of the bifidobacterium and the liquid of the bacillus pumilus on the three parts of the culture medium, wherein the volume ratio of the liquid to the culture medium is 3-5: 1, shaking the liquid and the crushed culture medium at the temperature of 30-37 ℃ for 10-15 min at 200-300 r/min to prepare three parts of bacterial suspension, placing the three parts of bacterial suspension into a sterile environment, culturing the three parts of bacterial suspension at the temperature of 30-37 ℃ for 48-72 h, and respectively amplifying three strains of the lactobacillus, the bifidobacterium and the bacillus pumilus;
s4: taking out the amplified strain, vibrating and crushing the strain by a centrifugal machine, performing filter pressing on the filtered residue and gauze, collecting the filter pressing liquid for filtering, combining the filtered filter pressing liquid and the filtrate to avoid waste of the bacterial liquid, and performing low-temperature atomization drying on the mixture to obtain powder of the strain;
s5: pulverizing processed radix astragali, radix et rhizoma Rhei, fructus forsythiae, radix Isatidis, herba Andrographitis, bupleuri radix, Prunellae Spica, flos Chrysanthemi Indici and herba Houttuyniae to 100-200 mesh, mixing the Chinese medicinal powder for 30-60 min, boiling for 60-120 min, centrifuging to extract Chinese medicinal essence, and spray drying to obtain Chinese medicinal essence powder;
s6: the strain powder and the Chinese herbal medicine essence powder are fully mixed according to the mixing mass ratio of the Chinese strain powder to the Chinese herbal medicine essence powder of 1-1.5: 2.5-3.5, and then are quantitatively packaged and stored in a cold storage mode.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (10)
1. A traditional Chinese medicine composite probiotic feed additive for broiler chickens comprises Chinese herbal medicine mixed powder and composite probiotic powder, and is characterized in that the Chinese herbal medicine mixed powder comprises the following components in percentage: 5-10% of astragalus membranaceus, 3-5% of rheum officinale, 5-10% of fructus forsythiae, 10-20% of isatis root, 1-5% of andrographis paniculata, 10-15% of radix bupleuri, 1-5% of selfheal, 5-20% of wild chrysanthemum flower and 35-40% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: 25-35% of peptone, 30-40% of agar, 10-25% of glucose, 3-5% of lactobacillus, 5-10% of bifidobacterium and 5-10% of bacillus pumilus.
2. The traditional Chinese medicine composite probiotic feed additive for broiler chickens of claim 1, which is characterized in that the Chinese herbal medicine mixed powder comprises the following components in percentage: 10% of astragalus membranaceus, 5% of rheum officinale, 10% of fructus forsythiae, 10% of isatis root, 2% of andrographis paniculata, 10% of radix bupleuri, 3% of selfheal, 15% of wild chrysanthemum flower and 35% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: peptone 25%, agar 30%, glucose 25%, lactobacillus 3%, bifidobacterium 8% and bacillus pumilus 9%.
3. The traditional Chinese medicine composite probiotic feed additive for broiler chickens of claim 1, which is characterized in that the Chinese herbal medicine mixed powder comprises the following components in percentage: 5% of astragalus membranaceus, 3% of rheum officinale, 10% of fructus forsythiae, 20% of isatis root, 4% of andrographis paniculata, 10% of radix bupleuri, 1% of selfheal, 10% of wild chrysanthemum flower and 37% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: 35% of peptone, 25% of agar, 20% of glucose, 4% of lactobacillus, 8% of bifidobacterium and 8% of bacillus pumilus.
4. The traditional Chinese medicine composite probiotic feed additive for broiler chickens and the preparation method thereof according to claim 1, characterized in that the Chinese herbal medicine mixed powder comprises the following components in percentage: 8% of astragalus membranaceus, 4% of rheum officinale, 8% of fructus forsythiae, 15% of isatis root, 5% of andrographis paniculata, 10% of radix bupleuri, 5% of selfheal, 20% of wild chrysanthemum flower and 25% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: 30% of peptone, 35% of agar, 15% of glucose, 5% of lactobacillus, 7% of bifidobacterium and 8% of bacillus pumilus.
5. The preparation method of the traditional Chinese medicine composite probiotic feed additive for broiler chickens according to claim 1, characterized by comprising the following steps:
s1: respectively dissolving lactobacillus, bifidobacterium and bacillus pumilus samples collected from chicken intestinal tracts in sterilized sterile water, shaking at the temperature of between 30 and 37 ℃ at the speed of between 150 and 300r/min for 30 to 60min, dispersing flora, and respectively preparing bacterial liquids of the lactobacillus, the bifidobacterium and the bacillus pumilus;
s2: taking peptone, glucose and agar according to mass percent, crushing the peptone and the agar, boiling, melting and mixing the peptone, the glucose and the agar, adjusting the pH value to 7.0 by using a sodium hydroxide solution, naturally cooling and sterilizing to obtain a culture medium;
s3: respectively taking liquid of lactobacillus, bifidobacterium and bacillus pumilus, dividing the prepared culture medium into three parts, crushing the culture medium, respectively placing the liquid of the lactobacillus, the liquid of the bifidobacterium and the liquid of the bacillus pumilus on the three parts of the culture medium to prepare three parts of bacterial suspension, placing the three parts of bacterial suspension into an aseptic environment, culturing for 48-72 h at 30-37 ℃, and respectively amplifying the three strains of the lactobacillus, the bifidobacterium and the bacillus pumilus;
s4: taking out the amplified strain, vibrating and crushing the strain by a centrifugal machine, and atomizing and drying the strain into powder at low temperature to obtain strain powder;
s5: pulverizing processed radix astragali, radix et rhizoma Rhei, fructus forsythiae, radix Isatidis, herba Andrographitis, bupleuri radix, Prunellae Spica, flos Chrysanthemi Indici and herba Houttuyniae to 100-200 mesh, mixing, boiling, centrifuging to extract Chinese herbal essence, and spray drying to obtain Chinese herbal essence powder;
s6: mixing the strain powder and the Chinese herbal medicine essence powder at a certain proportion, quantitatively packaging, and cold storing.
6. The preparation method of the traditional Chinese medicine composite probiotic feed additive for broiler chickens of claim 1, wherein the volume ratio of the bacterial liquid to the culture medium in S3 is 3-5: 1, the bacterial liquid and the crushed culture medium are shaken for 10min to 15min at the temperature of 30 ℃ to 37 ℃ at the speed of 200r/min to 300 r/min.
7. The preparation method of the traditional Chinese medicine composite probiotic feed additive for broiler chickens according to claim 1, wherein in S4, bacterial liquid needs to be filtered before atomization and drying, after 3-5 pieces of fine gauze are stacked, the bacterial suspension is filtered for 2-3 times until no residue is left on the fine gauze.
8. The preparation method of the traditional Chinese medicine composite probiotic feed additive for broiler chickens according to claim 1, wherein the residue filtered in S4 and gauze should be subjected to pressure filtration, and the pressure filtrate is collected and filtered, and the filtered pressure filtrate is combined with the filtrate to avoid waste of bacteria liquid.
9. The preparation method of the traditional Chinese medicine composite probiotic feed additive for broiler chickens according to claim 1, wherein in S5, the traditional Chinese medicine powder is mixed for 30min-60min, and the boiling time is 60min-120 min.
10. The preparation method of the traditional Chinese medicine composite probiotic feed additive for broiler chickens according to claim 1, wherein the mixing mass ratio of the strain powder and the Chinese herbal medicine essence powder in S6 is 1-1.5: 2.5-3.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210051213.1A CN114304429A (en) | 2022-01-17 | 2022-01-17 | Traditional Chinese medicine composite probiotic feed additive for broiler chickens and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210051213.1A CN114304429A (en) | 2022-01-17 | 2022-01-17 | Traditional Chinese medicine composite probiotic feed additive for broiler chickens and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114304429A true CN114304429A (en) | 2022-04-12 |
Family
ID=81028353
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210051213.1A Pending CN114304429A (en) | 2022-01-17 | 2022-01-17 | Traditional Chinese medicine composite probiotic feed additive for broiler chickens and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114304429A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114304428A (en) * | 2022-01-17 | 2022-04-12 | 清远一生自然生物研究院有限公司 | Preparation method of composite probiotic micro-ecological feed additive for meat ducks |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102599381A (en) * | 2012-03-29 | 2012-07-25 | 常熟市润丰农业有限公司 | Meat chicken feed additive |
| CN106165780A (en) * | 2016-07-18 | 2016-11-30 | 安徽奥力欣生物科技有限公司 | A kind of broiler Chinese medicine composite probiotics feed additive and preparation method thereof |
| CN106360040A (en) * | 2016-08-24 | 2017-02-01 | 广东海大集团股份有限公司畜牧水产研究中心 | Composite Chinese herbal medicine and probiotics feed additive for improving growth property and immuno-functions of broiler chicken |
| CN107156519A (en) * | 2017-06-12 | 2017-09-15 | 广东康达尔农牧科技有限公司 | A kind of feed addictive of effective regulation chicken intestinal health and preparation method thereof |
| CN107581392A (en) * | 2017-10-17 | 2018-01-16 | 肖秋花 | It is a kind of to be used to prevent and treat feed addictive of chicken colibacillosis and preparation method thereof |
| CN109965127A (en) * | 2017-12-28 | 2019-07-05 | 纪和春 | A kind of broiler chicken feed additive |
-
2022
- 2022-01-17 CN CN202210051213.1A patent/CN114304429A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102599381A (en) * | 2012-03-29 | 2012-07-25 | 常熟市润丰农业有限公司 | Meat chicken feed additive |
| CN106165780A (en) * | 2016-07-18 | 2016-11-30 | 安徽奥力欣生物科技有限公司 | A kind of broiler Chinese medicine composite probiotics feed additive and preparation method thereof |
| CN106360040A (en) * | 2016-08-24 | 2017-02-01 | 广东海大集团股份有限公司畜牧水产研究中心 | Composite Chinese herbal medicine and probiotics feed additive for improving growth property and immuno-functions of broiler chicken |
| CN107156519A (en) * | 2017-06-12 | 2017-09-15 | 广东康达尔农牧科技有限公司 | A kind of feed addictive of effective regulation chicken intestinal health and preparation method thereof |
| CN107581392A (en) * | 2017-10-17 | 2018-01-16 | 肖秋花 | It is a kind of to be used to prevent and treat feed addictive of chicken colibacillosis and preparation method thereof |
| CN109965127A (en) * | 2017-12-28 | 2019-07-05 | 纪和春 | A kind of broiler chicken feed additive |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114304428A (en) * | 2022-01-17 | 2022-04-12 | 清远一生自然生物研究院有限公司 | Preparation method of composite probiotic micro-ecological feed additive for meat ducks |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102872392B (en) | Chinese medicinal preparation for preventing and controlling swine mycoplasma pneumonia and preparation method thereof | |
| CN104304674B (en) | A kind of fermented type Chinese herb medicine additive for milk cow forage preventing recessive mastitis | |
| CN102091151B (en) | Traditional medicinal composition with wind-dispelling and dampness-removing function and preparation method thereof | |
| CN102920957A (en) | Chinese herbal medicine additive for preventing pig salmonellosis and pig feed using additive | |
| CN114304429A (en) | Traditional Chinese medicine composite probiotic feed additive for broiler chickens and preparation method thereof | |
| CN111150787A (en) | Traditional Chinese veterinary medicine premix and preparation method thereof | |
| CN106509438A (en) | Antiviral biological feed additive for pigs | |
| CN102058810A (en) | Lung-clearing asthma-relieving phlegm-reducing Chinese patent drug | |
| CN110251561B (en) | Antiviral compound traditional Chinese medicine preparation for pigs and preparation method thereof | |
| CN109646546B (en) | A Chinese medicinal composition for preventing and treating diarrhea of livestock, and its preparation method | |
| CN113181275A (en) | Fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis and preparation method thereof | |
| CN112972582A (en) | Traditional Chinese medicine composition for preventing and treating animal diarrhea and preparation method thereof | |
| CN112190686A (en) | Traditional Chinese medicine enzyme for replenishing qi and blood and improving immunity of livestock and poultry and preparation method thereof | |
| CN111358850A (en) | Traditional Chinese medicine composition for preventing and treating chicken colibacillosis and preparation method thereof | |
| CN115487250B (en) | Traditional Chinese medicine composition for preventing and treating African swine fever as well as preparation method and application thereof | |
| CN102144718B (en) | Selfheal extract feed additive and preparation method thereof | |
| CN113940966B (en) | Callicarpa nudiflora composition for treating escherichia coli infection and preparation method and application thereof | |
| CN111544570B (en) | Antiviral preparation added with antibacterial peptide and preparation method thereof | |
| CN117298210B (en) | Traditional Chinese medicine biological preparation for improving immunity of livestock and poultry as well as preparation method and application thereof | |
| CN108853195B (en) | Pharmaceutical composition for treating porcine epidemic diarrhea | |
| CN102144712B (en) | Platycodon root extract feed additive and preparation method thereof | |
| CN107028992B (en) | Compound propolis injection and preparation method thereof | |
| CN116508899A (en) | Feed additive for relieving swine stress and preparation method thereof | |
| CN116585423A (en) | Compound for improving organism immunity of poultry and application thereof | |
| CN114129619A (en) | Traditional Chinese medicine composition for preventing and treating infectious rhinitis of poultry and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220412 |