CN114196528A - PCR detector based on it is multispectral - Google Patents
PCR detector based on it is multispectral Download PDFInfo
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- CN114196528A CN114196528A CN202210055433.1A CN202210055433A CN114196528A CN 114196528 A CN114196528 A CN 114196528A CN 202210055433 A CN202210055433 A CN 202210055433A CN 114196528 A CN114196528 A CN 114196528A
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- 210000000078 claw Anatomy 0.000 claims abstract description 87
- 238000003753 real-time PCR Methods 0.000 claims abstract description 15
- 238000007599 discharging Methods 0.000 abstract description 7
- 238000001514 detection method Methods 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 4
- 230000008859 change Effects 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 description 39
- 239000000523 sample Substances 0.000 description 30
- 230000003321 amplification Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 230000007306 turnover Effects 0.000 description 3
- 230000004544 DNA amplification Effects 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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Abstract
The invention relates to the technical field of PCR detectors, in particular to a multispectral-based PCR detector which comprises a base, a control console and a real-time fluorescence quantitative PCR detector main body, wherein the control console and the real-time fluorescence quantitative PCR detector main body are respectively arranged on two sides of the top of the base, a moving platform and an electric telescopic supporting rod are arranged on the top of the control console, a first clamping claw and a second clamping claw are respectively arranged at two ends of the top of the moving platform, the integrated device has the function of automatically feeding and discharging pipes through the arranged moving platform, the electric telescopic supporting rod, the first clamping claw, the second clamping claw and a transverse frame which are combined with a first sliding seat and a second sliding seat, is more labor-saving and convenient, and has stable and reliable processes of feeding and discharging pipes, effectively avoids falling risks possibly occurring when the pipes are manually fed and discharged, and simultaneously provides a special operation table for PCR detection through the arranged control console, meanwhile, the transverse frame is convenient to rotate, change in direction and store through the rotating cylinder.
Description
Technical Field
The invention relates to the technical field of PCR detectors, in particular to a multispectral-based PCR detector.
Background
The PCR amplification apparatus is also called a PCR gene amplification apparatus, a PCR nucleic acid amplification apparatus, and a PCR technology for amplifying a specific DNA, and is widely used in medical and biological laboratories, for example, to determine whether a sample shows a map of a certain genetic disease, diagnosis of infectious diseases, gene replication, paternity testing, and the like, and can be classified into four types, i.e., a general PCR apparatus, a gradient PCR apparatus, an in-situ PCR apparatus, and a real-time fluorescence quantitative PCR apparatus, according to the purpose of DNA amplification and the standard of detection.
Wherein fluorescence quantitative PCR appearance has the single channel, binary channels and multichannel, when only using a fluorescence probe mark, choose the single channel for use, use the multichannel when having many fluorescence marks, the single channel also can detect many fluorescence's the target gene expression product of mark, because once can only detect the amplification amount of a target gene, need the multiple amplification just can detect the volume of different target gene fragments, mainly be used for medical clinical examination, biological medicine research and development, food industry, institutions such as scientific research institute, but the function of automatic business turn over pipe is not had to current fluorescence quantitative PCR appearance, need carry out artifical business turn over pipe when using, the risk that probably drops exists in the in-process of whole business turn over pipe.
Therefore, it is necessary to design a multispectral-based PCR detector to solve the above-mentioned problems in the background art.
Disclosure of Invention
The present invention is directed to a multispectral-based PCR detector to solve the above problems.
In order to achieve the purpose, the invention provides the following technical scheme:
a PCR detector based on multiple spectra comprises a base, a control console and a real-time fluorescence quantitative PCR instrument main body, wherein the control console and the real-time fluorescence quantitative PCR instrument main body are respectively installed on two sides of the top of the base, a moving platform and an electric telescopic supporting rod are installed on the top of the control console, a first clamping claw and a second clamping claw are respectively arranged at two ends of the top of the moving platform, a cross frame is arranged at the top end of the first clamping claw and the top end of the second clamping claw, the cross frame and the electric telescopic supporting rod are fixedly connected through a rotating cylinder, electromagnetic slide rails are respectively installed on the front side and the rear side of the cross frame in an embedded mode, the first clamping claw, the second clamping claw and the cross frame are respectively movably installed through a first sliding seat and a second sliding seat, and meanwhile the first clamping claw and the second clamping claw respectively comprise a vertical rod, a fixed claw, a movable claw and a telescopic cylinder.
As a preferable scheme of the invention, the fixed claw is fixedly installed at one side of the bottom end of the vertical rod, the movable claw is located at the top of the fixed claw, the movable claw is connected with the vertical rod in a sliding manner, the telescopic cylinder is installed between the fixed claw and the movable claw, an inclined end face is arranged at one end of the fixed claw, which is far away from the vertical rod, and the first clamping claw and the second clamping claw are symmetrically distributed at two ends of the top of the mobile platform.
As a preferable scheme of the present invention, a first cavity and a second cavity are respectively formed in the first slide seat and the second slide seat, a first electromagnetic slider and a second electromagnetic slider are respectively and fixedly mounted on one side of an inner wall of the first cavity and one side of an inner wall of the second cavity, and the first electromagnetic slider and the second electromagnetic slider are respectively and slidably connected to electromagnetic slide rails located on the front side and the rear side of the cross frame.
As a preferable scheme of the invention, the front side surface of the console is embedded with a digital display controller, and the top end of the console is provided with an operation table top.
As a preferable scheme of the invention, the center of the top surface of the real-time fluorescence quantitative PCR instrument main body is provided with a sample cell, the top of the sample cell is provided with a sample cell cover, and the back side surface of the sample cell is provided with a control panel.
As the preferable scheme of the invention, the top surface of the mobile platform is provided with a plurality of groups of pipe bottom grooves which are distributed at equal intervals.
Compared with the prior art, the invention has the beneficial effects that:
1. when the device is used, a test tube is placed on the top surface of a moving platform, the bottom end of the test tube is positioned in a tube bottom groove, then a PCR reaction system is led in on an operation table board, meanwhile, a digital display controller starts the whole device, a first sliding seat and a second sliding seat move on a cross frame until a first clamping claw and a second clamping claw are positioned at two sides of the test tube, a fixed claw is positioned at the bottom surface of two ends of the test tube, a movable claw is positioned at the top surface of two ends of the test tube, simultaneously, a telescopic cylinder starts to contract until the fixed claw and the movable claw clamp two ends of the test tube, then an electric telescopic support rod starts to expand to a certain height so that the test tube is higher than a sample pool, then the first sliding seat and the second sliding seat move towards one end close to the sample pool on the cross frame until the top surface of the sample pool is reached, simultaneously, the electric telescopic support rod contracts, and then the test tube gradually enters the interior of the sample pool, meanwhile, the telescopic cylinder starts to be unfolded, the movable claw is separated from the test tube, after the test tube is completely led into the inside of the sample pool, the first slide, the second slide moves towards the direction far away from the sample pool on the cross frame, the tube can be fed, otherwise, the tube can be taken, and the movable platform is arranged, the electric telescopic supporting rod is arranged, the first claw is clamped, the second clamping claw is clamped, the cross frame is combined with the first slide, the second slide can enable the whole device to have the function of automatic tube feeding and discharging, the labor is saved and convenient, meanwhile, the tube feeding and discharging process is stable and reliable, the falling risk possibly occurring when manual tube feeding and discharging is effectively avoided, meanwhile, the control console is not only used as a control hub station, a special operation table board is further provided for PCR detection, meanwhile, the cross frame is convenient to rotate and change for storage through the rotary cylinder arranged, and obstruction is avoided.
2. According to the invention, the bottom surfaces of the two ends of the test tube are conveniently guided in when the tube is taken through the arranged inclined end surfaces, so that the two ends of the test tube can be quickly moved to the top surfaces of the fixing claws.
Drawings
FIG. 1 is a schematic view of the overall front plan exterior structure of the present invention;
FIG. 2 is an enlarged structural view of a front view of a holding claw according to the present invention;
FIG. 3 is a side plan view of the slider of the present invention;
FIG. 4 is a top plan view of the mobile platform of the present invention;
in the figure: 1. a base; 2. a console; 21. a digital display controller; 22. an operation table top; 3. a real-time fluorescent quantitative PCR instrument main body; 31. a sample cell; 32. a sample cell cover; 33. a control panel; 4. a mobile platform; 41. a tube bottom groove; 5. an electric telescopic support rod; 6. a first clamping jaw; 61. a first slider; 611. a first cavity; 612. a first electromagnetic slider; 7. a second holding claw; 71. a second slide carriage; 711. a second cavity; 712. a second electromagnetic slider; 671. erecting a rod; 672. a fixed jaw; 673. a movable jaw; 674. a telescopic cylinder; 675. an inclined end surface; 8. a cross frame; 9. a rotating cylinder; 10. an electromagnetic slide rail.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without any creative work based on the embodiments of the present invention belong to the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution:
a PCR detector based on multiple spectra comprises a base 1, a control console 2 and a real-time fluorescence quantitative PCR instrument main body 3, wherein the control console 2 and the real-time fluorescence quantitative PCR instrument main body 3 are respectively installed on two sides of the top of the base 1, a moving platform 4 and an electric telescopic supporting rod 5 are installed on the top of the control console 2, a first clamping claw 6 and a second clamping claw 7 are respectively arranged at two ends of the top of the moving platform 4, a cross frame 8 is arranged at the top ends of the first clamping claw 6 and the second clamping claw 7, the cross frame 8 and the electric telescopic supporting rod 5 are fixedly connected through a rotating cylinder 9, electromagnetic slide rails 10 are respectively embedded into the front side and the rear side of the cross frame 8, the first clamping claw 6, the second clamping claw 7 and the cross frame 8 are respectively movably installed through a first slide seat 61 and a second slide seat 71, and the first clamping claw 6 and the second clamping claw 671, A fixed claw 672, a movable claw 673 and a telescopic cylinder 674, when in use, a test tube is placed on the top surface of the moving platform 4, the bottom end of the test tube is positioned in the bottom groove 41 of the test tube, then the introduction of a PCR reaction system is completed on the operation table top 22, meanwhile, the whole device is started through the digital display controller 21, the first sliding seat 61 and the second sliding seat 71 move on the cross frame 8 until the first clamping claw 6 and the second clamping claw 7 are positioned at two sides of the test tube, the fixed claw 672 is positioned at the bottom surfaces of two ends of the test tube, the movable claw 673 is positioned at the top surfaces of two ends of the test tube, meanwhile, the telescopic cylinder 674 starts to shrink until the fixed claw 672 and the movable claw 673 clamp two ends of the test tube, then the electric telescopic support rod 5 starts to expand to a certain height, so that the test tube is higher than the sample pool 31, then the first sliding seat 61 and the second sliding seat 71 move to one end close to the sample pool 31 on the cross frame 8 until the top surface of the sample pool 31 is reached, meanwhile, the electric telescopic supporting rod 5 contracts, the test tube gradually enters the sample pool 31 at the moment, meanwhile, the telescopic cylinder 674 starts to expand, the movable claw 673 is separated from the test tube, after the test tube is completely guided into the sample pool 31, the first sliding seat 61 and the second sliding seat 71 move on the cross frame 8 in the direction far away from the sample pool 31, the tube can be taken out, otherwise, the tube can be taken out, the integral device has the function of automatically taking in and out the tube through the arranged moving platform 4, the electric telescopic supporting rod 5, the first clamping claw 6, the second clamping claw 7, the cross frame 8, the first sliding seat 61 and the second sliding seat 71, the labor and the convenience are realized, meanwhile, the process of taking in and out the tube is stable and reliable, the falling risk possibly occurring in manual tube taking in and out is effectively avoided, meanwhile, the control console 2 is used as a control pivot station and also provides a special operation for PCR detection, meanwhile, the transverse frame 8 is convenient to rotate, change in direction and store through the rotating cylinder 9, and obstruction is avoided.
In an embodiment, referring to fig. 1, fig. 2, fig. 3 and fig. 4, a fixed claw 672 is fixedly installed at one side of the bottom end of an upright rod 671, a movable claw 673 is located at the top of the fixed claw 672, the movable claw 673 is slidably connected with the upright rod 671, a telescopic cylinder 674 is installed between the fixed claw 672 and the movable claw 673, one end of the fixed claw 672 far away from the upright rod 671 is provided with an inclined end face 675, and a first clamping claw 6 and a second clamping claw 7 are symmetrically distributed at two ends of the top of a movable platform 4; a first cavity 611 and a second cavity 711 are respectively formed in the first sliding seat 61 and the second sliding seat 71, a first electromagnetic slider 612 and a second electromagnetic slider 712 are respectively and fixedly mounted on one side of the inner wall of the first cavity 611 and the inner wall of the second cavity 711, and the first electromagnetic slider 612 and the second electromagnetic slider 712 are respectively and slidably connected with the electromagnetic slide rails 10 on the front side and the rear side of the cross frame 8; a digital display controller 21 is embedded in the front side surface of the console 2, and an operation table top 22 is arranged at the top end of the console 2; a sample cell 31 is arranged at the center of the top surface of the real-time fluorescence quantitative PCR instrument main body 3, a sample cell cover 32 is arranged at the top of the sample cell 31, and a control panel 33 is arranged on the rear side surface of the sample cell 31; moving platform 4 top surface is provided with the pipe bottom groove 41 that a plurality of groups equidistance distributes, conveniently imports to test tube both ends bottom surface when getting the pipe through the slope terminal surface 675 that sets up to faster make the test tube both ends remove to the top surface of stationary dog 672.
The working principle is as follows: when the device is used, a test tube is placed on the top surface of the moving platform 4, the bottom end of the test tube is positioned in the bottom groove 41 of the test tube, then the introduction of a PCR reaction system is completed on the operation table 22, meanwhile, the whole device is started through the digital display controller 21, the first sliding seat 61 and the second sliding seat 71 move on the cross frame 8 until the first clamping claw 6 and the second clamping claw 7 are positioned at two sides of the test tube, the fixed claw 672 is positioned at the bottom surfaces of two ends of the test tube, the movable claw 673 is positioned at the top surfaces of two ends of the test tube, meanwhile, the telescopic cylinder 674 starts to shrink until the fixed claw 672 and the movable claw 673 clamp two ends of the test tube, then the electric telescopic support rod 5 starts to expand to a certain height, so that the test tube is higher than the sample pool 31, then the first sliding seat 61 and the second sliding seat 71 move on the cross frame 8 to one end close to the sample pool 31 until the top surface of the sample pool 31 is reached, and simultaneously, the electric telescopic support rod 5 shrinks, at the moment, the test tube gradually enters the sample cell 31, meanwhile, the telescopic cylinder 674 starts to expand, the movable claw 673 is separated from the test tube, after the test tube is completely guided into the sample cell 31, the first slide seat 61 and the second slide seat 71 move on the cross frame 8 in the direction far away from the sample cell 31, the tube feeding can be completed, otherwise, the tube taking can be performed, the integral device has the function of automatically feeding and discharging tubes through the arranged moving platform 4, the electric telescopic supporting rod 5, the first clamping claw 6, the second clamping claw 7 and the cross frame 8 which are combined with the first slide seat 61 and the second slide seat 71, the labor and the convenience are realized, meanwhile, the process of feeding and discharging tubes is stable and reliable, the falling risk which possibly occurs when the tubes are manually fed and discharged is effectively avoided, meanwhile, the arranged control console 2 not only serves as a control pivot station, but also provides a special operation table for PCR detection, meanwhile, the cross frame 8 is conveniently rotated and stored through the arranged rotary cylinder 9, avoid causing the hindrance, and the slope terminal surface 675 through the setting is convenient to be leading-in to test tube both ends bottom surface when getting the pipe to faster make the test tube both ends remove to the top surface of stationary dog 672, have certain spreading value.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The multispectral PCR detector comprises a base (1), a console (2) and a real-time fluorescence quantitative PCR detector main body (3), and is characterized in that: control cabinet (2) are installed respectively to the top both sides of base (1), real-time fluorescence quantitative PCR appearance main part (3), install moving platform (4) and electronic flexible bracing piece (5) at the top of control cabinet (2), and the top both ends of moving platform (4) are provided with first with respectively holding claw (6), the second adds and holds claw (7), simultaneously first with hold claw (6), the top that the second adds and holds claw (7) is provided with crossbearer (8), meanwhile crossbearer (8) and electronic flexible bracing piece (5) are through revolving cylinder (9) fixed connection, and electromagnetic slide rail (10) are all installed in the embedding of both sides around crossbearer (8), simultaneously first add hold claw (6), the second adds and holds claw (7) and crossbearer (8) and respectively through first slide (61), second slide (71) movable mounting, meanwhile first add hold claw (6), The second clamping claws (7) respectively comprise upright rods (671), fixed claws (672), movable claws (673) and telescopic cylinders (674).
2. The multispectral-based PCR detector of claim 1, wherein: stationary dog (672) fixed mounting is in one side of pole setting (671) bottom, and moveable claw (673) are located the top of stationary dog (672), and moveable claw (673) and pole setting (671) sliding connection simultaneously, and install between stationary dog (672) and moveable claw (673) simultaneously telescopic cylinder (674), and stationary dog (672) keep away from pole setting (671) one end and are provided with slope terminal surface (675), simultaneously first add and hold claw (6), second add and hold claw (7) and be the top both ends of symmetric distribution at moving platform (4).
3. The multispectral-based PCR detector of claim 1, wherein: first cavity (611) and second cavity (711) have been seted up respectively to the inside of first slide (61), second slide (71), and the inner wall one side of first cavity (611), second cavity (711) is fixed mounting respectively has first electromagnetism slider (612), second electromagnetism slider (712), and first electromagnetism slider (612), second electromagnetism slider (712) respectively with be located electromagnetic slide rail (10) sliding connection of crossbearer (8) front and back both sides simultaneously.
4. The multispectral-based PCR detector of claim 1, wherein: the front side surface of the control console (2) is embedded with a digital display controller (21), and the top end of the control console (2) is provided with an operation table top (22).
5. The multispectral-based PCR detector of claim 1, wherein: a sample cell (31) is arranged at the center of the top surface of the real-time fluorescence quantitative PCR instrument main body (3), a sample cell cover (32) is installed at the top of the sample cell (31), and a control panel (33) is arranged on the rear side surface of the sample cell (31).
6. The multispectral-based PCR detector of claim 1, wherein: the top surface of the mobile platform (4) is provided with a plurality of groups of pipe bottom grooves (41) distributed at equal intervals.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210055433.1A CN114196528A (en) | 2022-01-18 | 2022-01-18 | PCR detector based on it is multispectral |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210055433.1A CN114196528A (en) | 2022-01-18 | 2022-01-18 | PCR detector based on it is multispectral |
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| CN114196528A true CN114196528A (en) | 2022-03-18 |
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