CN114042482B - Low-adsorption antibacterial centrifuge tube and application thereof - Google Patents
Low-adsorption antibacterial centrifuge tube and application thereof Download PDFInfo
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Abstract
The invention discloses a low-adsorption and antibacterial centrifugal tube and application thereof, and relates to the technical field of centrifugal tubes. The preparation method of the low-adsorption antibacterial centrifuge tube comprises the following steps: pretreating the surface of a centrifugal tube, namely sequentially removing oil and organic impurities from a commercially available centrifugal tube, and then hydroxylating the surface of the centrifugal tube; coating the surface of the centrifugal tube, namely dissolving the modified silane coupling agent in methanol, coating the modified silane coupling agent on the surface of the centrifugal tube subjected to hydroxylation, airing, and carrying out heat treatment to obtain the coated centrifugal tube. The centrifugal tube prepared by the invention has excellent biological adhesion resistance, good stability, low adsorption performance and good antibacterial performance; still have excellent hydrophobic property at high temperature, improve the liquid wall built-up phenomenon.
Description
Technical Field
The invention belongs to the technical field of centrifugal tubes, and particularly relates to a low-adsorption antibacterial centrifugal tube and application thereof.
Background
At present, all low-adsorption and antibacterial PP technologies for biomedical research on the market are monopolized abroad, the product price is high, and the purchase period is long. The domestic work is dedicated to the development of the materials for years, obvious breakthroughs are made in some fields, but the quality is still deficient, for example, a thin film residue exists in a container or during liquid transfer, and precious experimental materials are wasted; at high temperature, although the surface tension of the plastic product is reduced, the added material in the plastic product is precipitated and discolored; the common nano silver particle antibacterial agent has oxidation failure and the like due to long experimental period, so that the use of domestic materials is limited.
Disclosure of Invention
The invention aims to provide a low-adsorption and antibacterial centrifugal tube and application thereof, wherein the centrifugal tube has excellent antibacterial performance and anti-bioadhesion performance, and is excellent in wear resistance, acid and alkali resistance and heat-resistant stability.
The technical scheme adopted by the invention for realizing the purpose is as follows:
a modified silane coupling agent has a structure shown in formula I:
The preparation method of the modified silane coupling agent comprises the step of reacting 6-shogaol with triethoxysilane to generate the modified silane coupling agent.
Further, the preparation method of the modified triethoxysilane comprises the following specific steps:
dissolving 6-shogaol in toluene, reacting, heating to 50-60 ℃, adding a karstedt catalyst, and continuously stirring for 40-60 min; then adding triethoxy silane into the constant-pressure dropping funnel, slowly dropping into the solution, and reacting at a constant temperature of 75-80 ℃ for 20-25 h after dropping; and carrying out suction filtration while the mixture is hot, cooling to room temperature, carrying out rotary evaporation, drying, and recrystallizing with ethanol to obtain the modified triethoxysilane.
The mass ratio of 6-shogaol to triethoxysilane is 1: 0.48 to 0.62; the solid-to-liquid ratio of 6-shogaol to toluene is 1 g: 30-35 mL; the solid-to-liquid ratio of the 6-shogaol to the karstedt catalyst is 3 g: 0.12-0.2 mL.
The invention also discloses the application of the modified silane coupling agent in the preparation of an anti-biological adhesion biomaterial or a centrifugal tube surface treatment process.
The modified silane coupling agent is used for enhancing the antibacterial property and the heat resistance of a centrifugal tube.
A method for preparing a low-adsorption antibacterial centrifuge tube comprises the following steps:
pretreating the surface of a centrifugal tube, namely sequentially removing oil and organic impurities from the centrifugal tube, and then hydroxylating the surface of the centrifugal tube;
and (3) coating the surface of the centrifugal tube, namely dissolving the modified silane coupling agent in methanol to prepare a solution with the concentration of 8-12 wt%, coating the solution on the surface of the centrifugal tube subjected to hydroxylation, airing, and performing heat treatment to obtain the coated centrifugal tube. Due to the existence of the modified silane coupling agent in the coating, an ordered layered structure is spontaneously formed on an interface through chemisorption and chemical bonding generated between a reactive group of a compound and a substrate and is firmly combined on the surface of a solid base material, the film forming effect and the stability are good, and excellent antibacterial capacity is obtained; and the air thin layer adsorbed by the micro/nano structure on the surface is used as a physical barrier, and the biological adhesion resistance performance is good. Meanwhile, the 6-shogaol modified silane coupling agent forms a thin film layer on the surface of the centrifuge tube base material, so that the wear resistance and heat resistance of the surface of the centrifuge tube are obviously improved, and the stability of the coating is obviously enhanced.
The hydroxylation treatment in the centrifuge tube surface pretreatment is specifically performed by: preparing 1.5-2 wt% of acetone solution of benzophenone, placing the acetone solution into a centrifugal tube, soaking for 10-15 min, taking out, placing the acetone solution under an ultraviolet lamp for irradiating for 25-30 s, rinsing with acetone, and drying at 50-60 ℃ under normal pressure; wherein the wavelength of the ultraviolet lamp is 254nm, and the intensity of the ultraviolet light is 6000-6500 mu W-cm-2。
The coating thickness is 0.02-0.2 mm.
The method is characterized in that 1.2-2.8 wt% of epoxy decursin is added in the centrifugal tube surface coating treatment process. The addition of the epoxy decursin can further improve the hydrophobicity of the centrifugal tube; the antibacterial agent is compounded with a modified silane coupling agent for use, so that the antibacterial activity of the centrifugal tube is obviously enhanced; the existence of the epoxy resin can effectively improve the acid and alkali resistance stability of the surface of the centrifugal tube, and the excellent hydrophobic property can be still maintained under the conditions of strong acid and strong alkali.
Further, 0.8-1.2 wt% of N- [ 4-cyano-3- (trifluoromethyl) phenyl ] methyl epoxy acrylamide is added in the centrifugal tube surface coating treatment process. The existence of the N- [ 4-cyano-3- (trifluoromethyl) phenyl ] methyl epoxy acrylamide can effectively improve the hydrophobic property and the impact resistance of the surface of the centrifugal tube, further improve the wear resistance and the acid and alkali resistance of the surface of the centrifugal tube, and has a synergistic enhancement effect when being compounded with the epoxy decursin. In addition, the N- [ 4-cyano-3- (trifluoromethyl) phenyl ] methyl epoxy acrylamide and the modified silane coupling agent are compounded to further improve the anti-bioadhesion property of the surface of the centrifugal tube.
The invention also discloses the low-adsorption and antibacterial centrifugal tube prepared by the preparation method.
The invention also discloses the application of the low-adsorption and antibacterial centrifugal tube in a real-time fluorescence quantitative PCR instrument.
Compared with the prior art, the invention has the following beneficial effects:
the 6-shogaol modified silane coupling agent is adopted, so that the hydrophobic property of the silane coupling agent can be effectively improved, the silane coupling agent is coated on the surface of a glass tube, the modified silane coupling agent in the coating is firmly combined on the surface of a solid substrate, the film forming effect and the stability are good, and the excellent antibacterial ability is obtained; and excellent anti-bioadhesion performance. Meanwhile, the 6-shogaol modified silane coupling agent forms a thin film layer on the surface of the centrifuge tube base material, so that the wear resistance and heat resistance of the surface of the centrifuge tube are obviously improved, and the stability of the coating is obviously enhanced. The addition of the epoxy decursin can further improve the hydrophobicity of the centrifugal tube, and the epoxy decursin is compounded with the modified silane coupling agent for use, so that the antibacterial activity of the centrifugal tube is obviously enhanced; can effectively improve the acid and alkali resistance stability of the surface of the centrifugal tube. In addition, the hydrophobic property and the impact resistance of the surface of the centrifugal tube can be effectively improved by adding the N- [ 4-cyano-3- (trifluoromethyl) phenyl ] methyl epoxy acrylamide, the wear resistance and the acid and alkali resistance of the surface of the centrifugal tube are further improved, and the N- [ 4-cyano-3- (trifluoromethyl) phenyl ] methyl epoxy acrylamide has a synergistic enhancement effect by compounding with the epoxy decursite; and the compound with the modified silane coupling agent further improves the biological adhesion resistance of the surface of the centrifugal tube.
Therefore, the present invention provides a low-adsorption, antibacterial centrifuge tube having excellent antibacterial performance and anti-bioadhesion property, and excellent wear resistance, acid and alkali resistance, and heat stability, and its use.
Detailed Description
The technical solution of the present invention is further described in detail with reference to the following embodiments:
example 1:
preparation of modified silane coupling agent:
dissolving 6-shogaol in toluene (the solid-to-liquid ratio of 6-shogaol to toluene is 1 g: 33 mL), reacting, heating to 55 ℃, adding karstedt catalyst (the solid-to-liquid ratio of 6-shogaol to karstedt catalyst is 3 g: 0.14 mL), and continuing stirring for 50 min; then adding triethoxy silane (the mass ratio of 6-shogaol to triethoxy silane is 1: 0.51) into a constant pressure dropping funnel, slowly dropping into the solution, and reacting at a constant temperature of 80 ℃ for 23 hours after dropping; and carrying out suction filtration while the mixture is hot, cooling to room temperature, carrying out rotary evaporation, drying, and recrystallizing with ethanol to obtain the modified triethoxysilane.
Preparation of a low-adsorption antibacterial centrifuge tube:
pretreating the surface of a centrifugal tube, cleaning the commercially available centrifugal tube for several times by using a detergent to remove most of oil on the surface, and then fully leaching by using tap water and distilled water respectively; then ultrasonically cleaning the culture medium by using absolute ethyl alcohol, acetone and ultrapure water, finally washing the culture medium by using a large amount of ultrapure water, drying the culture medium by blowing, and placing the culture medium in a clean culture dish for later use; preparing acetone solution of 2wt% benzophenone, placing into a centrifuge tube, soaking for 10min, taking out, and placing under an ultraviolet lamp (ultraviolet light intensity of 6500 μ W cm with λ =254 nm)-2) Irradiating for 30s, rinsing with acetone, and drying at 60 deg.C under normal pressure;
coating the surface of a centrifugal tube, dissolving a modified silane coupling agent in methanol to prepare a solution with the concentration of 10wt%, dripping a proper amount of distilled water, adjusting the pH of the solution to 4 by using 0.1M hydrochloric acid solution, hydrolyzing and aging for 5 hours, coating the surface of the hydroxylated base material with a film, naturally airing, placing the dried base material in an oven, and performing heat treatment at 108 ℃ for 1 hour to obtain the coated centrifugal tube. Wherein the thickness of the coating is 0.12 mm.
Example 2:
the modified silane coupling agent was prepared differently from example 1 in that: the mass ratio of the 6-shogaol to the triethoxysilane is 1: 0.58; the solid-to-liquid ratio of the 6-shogaol to the karstedt catalyst is 3 g: 0.17 mL.
The preparation of a low adsorption, antibacterial centrifuge tube differs from example 1 in that: the modified silane coupling agent was obtained in this example.
Example 3:
the modified silane coupling agent was prepared differently from example 1 in that: the mass ratio of the 6-shogaol to the triethoxysilane is 1: 0.55; the solid-to-liquid ratio of the 6-shogaol to the karstedt catalyst is 3 g: 0.19 mL.
The preparation of a low adsorption, antibacterial centrifuge tube differs from example 1 in that: the modified silane coupling agent was obtained in this example.
Example 4:
the modified silane coupling agent was prepared differently from example 1 in that: the mass ratio of the 6-shogaol to the triethoxysilane is 1: 0.60; the solid-to-liquid ratio of the 6-shogaol to the karstedt catalyst is 3 g: 0.15 mL.
The preparation of a low adsorption, antibacterial centrifuge tube differs from example 1 in that: the modified silane coupling agent was obtained in this example.
Example 5:
the modified silane coupling agent was the same as in example 1.
The preparation of a low adsorption, antibacterial centrifuge tube differs from example 1 in that: to the methanol solution was added 1.62wt% of epoxy decursin ether.
Example 6:
the modified silane coupling agent was the same as in example 1.
The preparation of a low adsorption, antibacterial centrifuge tube differs from example 1 in that: to the methanol solution was added N- [ 4-cyano-3- (trifluoromethyl) phenyl ] methyl epoxy acrylamide at a concentration of 0.92% by weight.
Example 7:
the modified silane coupling agent was the same as in example 1.
The preparation of a low adsorption, antibacterial centrifuge tube differs from example 1 in that: 1.62% by weight of epoxy decursin and 0.92% by weight of N- [ 4-cyano-3- (trifluoromethyl) phenyl ] methyl epoxy acrylamide were added to the methanol solution.
Example 8:
the preparation of a low adsorption, antibacterial centrifuge tube differs from example 5 in that: triethoxy silane is adopted to replace a modified silane coupling agent.
Example 9:
the preparation of a low adsorption, antibacterial centrifuge tube differs from example 6 in that: triethoxysilane is used to replace the modified silane coupling agent.
Example 10:
a low sorption, antimicrobial centrifuge tube was prepared as in example 7 with the following differences: triethoxysilane is used to replace the modified silane coupling agent.
Comparative example 1:
the preparation of a low adsorption, antibacterial centrifuge tube differs from example 1 in that: triethoxysilane is used to replace the modified silane coupling agent.
Test example 1:
1. characterization of nuclear magnetic resonance (1H NMR）
Weighing 3mg of sample, dissolving the sample in DMSO, preparing a sample solution, placing the sample solution in a nuclear magnetic resonance instrument, and measuring. The operating conditions of the instrument are as follows: AVANCE III 400 nuclear magnetic resonance apparatus (Bruker). And analyzing the type and the amount of hydrogen in the target product through the data of the hydrogen spectrum.
The modified silane coupling agent prepared in example 1 was subjected to nuclear magnetic hydrogen spectrum test, and the characterization results were as follows:
example 1:1H NMR（400 MHz，DMSO-d6）：δ：6.82（d，1H，Ar-H），6.75（d，1H，Ar-H），6.69（d，1H，Ar-H），5.45（s，1H，-OH），3.87（m，9H，O-CH 3 and O-CH 2），2.8~2.85（m，4H，Ar-CH 2-CH 2），2.62、2.35（m，2H，O=C-CH 2），1.68（m，1H，Si-CH），1.26~1.31（m，8H，Si-CH-CH 2-CH 2-CH 2-CH 2），1.24（t，9H，Si-O-CH2-CH 3），0.91（t，3H，Si-CH-(CH2)4-CH 3). Indicating that the modified silane coupling agent is successfully prepared.
2. Surface wettability test
And measuring the contact angle CA of the deionized water in the air on the surface of the material by using a contact angle measuring instrument, and collecting contact angle photos, wherein the experimental result is the average value of three tests. Untreated centrifuge tubes (commercially available centrifuge tubes were washed several times with detergent to remove most of the oil on the surface, then rinsed thoroughly with tap water and distilled water, respectively, then ultrasonically washed with absolute ethanol, acetone and ultrapure water, finally washed with a large amount of ultrapure water, and blown dry) were used as a control group.
The results of the above tests on the centrifuge tubes prepared in comparative example 1 and examples 1 to 10 are shown in Table 1:
TABLE 1 Water contact Angle test results
As can be seen from the analysis in Table 1, the water contact angle of the centrifugal tube prepared in example 1 is higher than that of comparative example 1 and is far better than that of a control group, which indicates that a thin film layer is constructed on the surface of a centrifugal tube substrate by adopting a 6-shogaol modified silane coupling agent, and the hydrophobic property of the surface of the centrifugal tube is obviously improved; the effects of example 5 and example 6 are slightly better than those of example 1, the effect of example 7 is not significantly different from those of examples 5 to 6, the effects of examples 8 and example 9 are better than those of comparative example 1, and the effect of example 10 is equivalent to those of examples 8 to 9, which shows that the presence of the epoxy decursin and/or N- [ 4-cyano-3- (trifluoromethyl) phenyl ] methyl epoxy acrylamide has a positive effect on the hydrophobic property.
3. Coating stability test
The surface of the material is rubbed by using sand paper (polished for 15 times by using 80-mesh sand paper), the stability of the surface in a heating state (the heating temperature is 150 ℃ and the heating time is 2 hours) and whether the original super-hydrophobicity (the reduction rate of a water contact angle) can be achieved after the material is soaked for 24 hours under the conditions of strong acid and strong alkali are researched to represent the stability of the coating.
The results of the above tests on the centrifuge tubes prepared in comparative example 1 and examples 1 to 10 are shown in Table 2:
table 2 stability test results
From the analysis in table 2, it can be seen that the water contact angle reduction rate of the centrifugal tube prepared in example 1 after being subjected to abrasive paper friction and heating treatment is obviously lower than that of comparative example 1, and the water contact angle reduction rate of the centrifugal tube after being subjected to strong acid and strong alkali treatment is not obviously different from that of comparative example 1, which indicates that the 6-shogaol modified silane coupling agent is adopted to construct a thin film layer on the surface of the centrifugal tube substrate, so that the wear resistance and heat resistance of the surface of the centrifugal tube are obviously improved, but the wear resistance and heat resistance of the centrifugal tube are not positively influenced. Compared with the centrifuge tube prepared in the embodiment 1, the drop rate of the water contact angle of the centrifuge tube after being subjected to abrasive paper friction and heating treatment is not obviously different, the drop rate of the centrifuge tube after being subjected to strong acid and strong alkali treatment is obviously reduced, and the effect of the centrifuge tube prepared in the embodiment 8 is consistent with that of the centrifuge tube prepared in the embodiment 5, which shows that the acid and alkali stability of the surface of the centrifuge tube can be effectively improved due to the existence of the epoxy resin. The water contact angle reduction rate of the centrifugal tube prepared in the embodiment 6 after being rubbed by sandpaper and treated by strong acid and strong alkali is obviously reduced compared with that of the centrifugal tube prepared in the embodiment 1, the reduction rate is not obviously changed after being heated, and the effect of the embodiment 9 is consistent with that of the embodiment 6, which shows that the wear resistance and the acid and alkali resistance of the surface of the centrifugal tube can be further improved by the existence of N- [ 4-cyano-3- (trifluoromethyl) phenyl ] methyl epoxy acrylamide. The reduction rate of the water contact angle of the centrifugal tube prepared in the example 7 after being rubbed by sandpaper and treated by strong acid and strong alkali is obviously reduced compared with the reduction rate of the centrifugal tube prepared in the examples 5 and 6, the reduction rate of the centrifugal tube after being treated by heating is not obviously changed, and the effect of the example 10 is consistent with that of the example 7, which shows that the compounding of the epoxy decursin and the N- [ 4-cyano-3- (trifluoromethyl) phenyl ] methyl epoxy acrylamide has a synergistic enhancement effect on the wear resistance and the acid and alkali resistance of the surface of the centrifugal tube.
4. Test for anti-adhesion Property
Bovine Serum Albumin (BSA) and Ovalbumin (OVA) were mixed at a concentration of 3mg/mL at 25 ℃ experimental temperature. And soaking a sample to be detected in the protein solution for 48 hours, taking out the sample, slightly cleaning the surface of the sample by using distilled water, and eluting the protein which is not firmly adhered. And finally, drying the surface of the sample after the protein is adhered at normal temperature, and carrying out contact angle experimental test.
The results of the above tests on the centrifuge tubes prepared in comparative example 1 and examples 1 to 10 are shown in table 3:
TABLE 3 anti-adhesion test results
Analysis in table 3 shows that after the centrifuge tube prepared in example 1 is soaked in the protein mixed solution, the reduction rate of the water contact angle is obviously lower than that of comparative example 1, which indicates that the 6-shogaol modified silane coupling agent is adopted to construct a thin film layer on the surface of the centrifuge tube substrate, so that the biological adhesion resistance of the surface of the centrifuge tube is obviously enhanced; the effect of example 6 is better than that of example 1, the effect of example 7 is better than that of example 6, and the effects of examples 9 and 10 are slightly better than that of comparative example 1, which shows that the existence of N- [ 4-cyano-3- (trifluoromethyl) phenyl ] methyl epoxy acrylamide can enhance the biological viscosity resistance of the centrifugal tube to a certain extent, and the biological viscosity resistance can be better and the stability is better when the N- [ 4-cyano-3- (trifluoromethyl) phenyl ] methyl epoxy acrylamide is compounded with the modified silane coupling agent.
5. Test of antibacterial Property
The antibacterial adhesion effect on the surface of the prepared material was examined, and representative, typical gram-positive bacteria staphylococcus aureus (s. aureus, ATCC 29213) and gram-negative bacteria escherichia coli (e.coli, ATCC 8739) were selected as test strains. And (4) determining standard curves of different bacterial concentrations and absorbance OD values.
Preparation of bacterial suspensions
Taking a standard strain tube, inoculating a little strain to sterilized nutritious meat by using an inoculating loop under the aseptic conditionCulturing the broth in the soup at 35-37 ℃ for 18-24 h to obtain a nutrient broth bacterial suspension (concentrated bacterial liquid); inoculating the bacterial solution to a nutrient agar plate by using an inoculating loop, culturing for 18-24 h at 35-37 ℃, selecting a single colony with good growth, inoculating the single colony to a nutrient broth culture medium, culturing for 48h under the same condition, and adjusting the concentration of a bacterial suspension to 1 × 10 by using sterilized normal saline9CFU/L for standby.
And (3) testing antibacterial performance:
the concentration of bacteria in the bacterial suspension after a certain time of contact with the sample is determined spectrophotometrically. The method comprises the following specific steps of: the surfaces of the centrifuge tubes of the sample and the control sample were washed with 75vol% ethanol, sterilized by UV light, and then placed in a 1.6mL chamber containing 1X 103CFU/L of nutrient broth bacterial suspension in 24-well bacterial culture plates, after gentle shaking, was incubated at 37 ℃. After incubation for 12h and 24h, respectively, the bacterial concentration in the bacterial suspension after contact with the sample was determined spectrophotometrically. Since the concentration of the bacterial suspension is in direct proportion to the absorbance in a certain range, the absorbance can be used for reflecting the bacterial concentration.
The results of the above tests on the centrifuge tubes prepared in comparative example 1 and examples 1 to 10 are shown in Table 4:
TABLE 4 results of the bacteriostatic properties test
From the analysis in table 4, it can be seen that the centrifuge tube prepared in example 1 has excellent antibacterial performance, and the sterilization rate of staphylococcus aureus and escherichia coli is obviously higher than that of comparative example 1 and the control group, which indicates that the 6-shogaol modified silane coupling agent is adopted to construct a thin film layer on the surface of the centrifuge tube substrate, so that the antibacterial performance of the surface of the centrifuge tube is obviously enhanced; the effect of example 5 is better than that of example 1, the effect of example 7 is not significantly different from that of example 5, better than that of example 6, the effect of example 8 is better than that of comparative example 1, and the effect of example 10 is better than that of example 9, which shows that the antibacterial property of the centrifugal tube can be obviously enhanced due to the existence of the epoxy decursin, and the antibacterial effect can be better when the epoxy decursin is compounded with the modified silane coupling agent.
Test example 2:
notched impact test
According to the requirements of national standard GB/T1843-.
The results of the above tests on the centrifuge tubes prepared in comparative example 1 and examples 1 to 10 are shown in table 5:
TABLE 5 impact resistance test results
| Sample (I) | Notched impact strength (kJ/m)2） |
| Control group | 3.09 |
| Comparative example 1 | 3.42 |
| Example 1 | 3.61 |
| Example 2 | 3.50 |
| Example 3 | 3.60 |
| Example 4 | 3.47 |
| Example 5 | 3.69 |
| Example 6 | 5.57 |
| Example 7 | 8.23 |
| Example 8 | 3.49 |
| Example 9 | 5.36 |
| Example 10 | 7.95 |
From the analysis in table 5, it can be seen that the notched impact strength of the centrifugal tube prepared in example 1 is equivalent to that of comparative example 1, the effects of examples 5 and 6 are better than those of example 1, the effect of example 7 is significantly higher than those of examples 5 and 6, the effect of example 8 is better than that of comparative example 1, and the effect of example 10 is significantly better than those of examples 8 to 9, which indicates that the existence of N- [ 4-cyano-3- (trifluoromethyl) phenyl ] methyl epoxy acrylamide can effectively improve the impact resistance of the centrifugal tube, and the impact resistance of the centrifugal tube can be synergistically enhanced by the compound use of N- [ 4-cyano-3- (trifluoromethyl) phenyl ] methyl epoxy acrylamide and epoxy resin.
Conventional techniques in the above embodiments are known to those skilled in the art, and therefore, will not be described in detail herein.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (9)
1. A method for preparing a low-adsorption antibacterial centrifuge tube comprises the following steps:
pretreating the surface of a centrifugal tube, namely sequentially removing oil and organic impurities from the centrifugal tube, cleaning the centrifugal tube, and then hydroxylating the surface of the centrifugal tube;
coating the surface of the centrifugal tube, namely dissolving a modified silane coupling agent in methanol, coating the modified silane coupling agent on the surface of the centrifugal tube subjected to hydroxylation, airing, and carrying out heat treatment to obtain the coated centrifugal tube;
the hydroxylation treatment in the centrifuge tube surface pretreatment specifically comprises the following operations: preparing 1.5-2 wt% of acetone solution of benzophenone, placing the acetone solution into a centrifugal tube, soaking for 10-15 min, taking out, placing the acetone solution under an ultraviolet lamp for irradiating for 25-30 s, rinsing with acetone, and drying at 50-60 ℃ under normal pressure; wherein the wavelength of the ultraviolet lamp is 254nm, and the intensity of the ultraviolet light is 6000-6500 mu W-cm-2；
The structure of the modified silane coupling agent is shown as the formula I:
2. the method of claim 1, wherein the method comprises the steps of: the modified silane coupling agent is generated by the reaction of 6-shogaol and triethoxysilane.
3. The method for preparing a low-adsorption antibacterial centrifuge tube according to claim 1, wherein the method comprises the following steps: the modified silane coupling agent is used in the surface treatment process for preparing the anti-biological adhesion biological material or the centrifugal tube.
4. The method of claim 3, wherein the method comprises the steps of: the modified silane coupling agent is used for enhancing the antibacterial property and the heat resistance of a centrifugal tube.
5. The method for preparing a low-adsorption antibacterial centrifuge tube according to claim 1, wherein the method comprises the following steps: the thickness of the coating is 0.02-0.2 mm.
6. The method for preparing a low-adsorption antibacterial centrifuge tube according to claim 1, wherein the method comprises the following steps: 1.2-2.8 wt% of epoxy decursin ether is also added in the treatment process of the centrifugal tube surface coating.
7. The method of claim 1 or claim 6, wherein the method comprises the steps of: and 0.8-1.2 wt% of N- [ 4-cyano-3- (trifluoromethyl) phenyl ] methyl epoxy acrylamide is also added in the process of coating the surface of the centrifugal tube.
8. A low-adsorption antibacterial centrifuge tube prepared by the preparation method of any one of claims 1 to 7.
9. The low-adsorption, antibacterial centrifuge tube of claim 8 is used in a real-time fluorescent quantitative PCR instrument.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6032705A (en) * | 1983-08-02 | 1985-02-19 | Shiraimatsu Shinyaku Kk | Antimicrobial agent |
| JPH05132065A (en) * | 1991-11-05 | 1993-05-28 | Nichiden Rika Glass Hanbai Kk | Surface-treated glass bottle |
| CN201389462Y (en) * | 2009-04-10 | 2010-01-27 | 罗耿荣 | Antibacterial pipette |
| CN202803265U (en) * | 2012-09-30 | 2013-03-20 | 刘长海 | Novel storage container for objects to be detected |
| CN104086088A (en) * | 2014-06-07 | 2014-10-08 | 北京化工大学 | Method of preparing glass with surface antibacterial property by photopolymerization |
| CN107474617A (en) * | 2017-07-15 | 2017-12-15 | 佛山实瑞先导材料研究院（普通合伙） | A kind of aqueous, environmental protective antibacterial antifouling paint |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20040126280A1 (en) * | 2002-12-31 | 2004-07-01 | Leaman Donald H. | Method and apparatus for preserving urine specimens at room temperature |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6032705A (en) * | 1983-08-02 | 1985-02-19 | Shiraimatsu Shinyaku Kk | Antimicrobial agent |
| JPH05132065A (en) * | 1991-11-05 | 1993-05-28 | Nichiden Rika Glass Hanbai Kk | Surface-treated glass bottle |
| CN201389462Y (en) * | 2009-04-10 | 2010-01-27 | 罗耿荣 | Antibacterial pipette |
| CN202803265U (en) * | 2012-09-30 | 2013-03-20 | 刘长海 | Novel storage container for objects to be detected |
| CN104086088A (en) * | 2014-06-07 | 2014-10-08 | 北京化工大学 | Method of preparing glass with surface antibacterial property by photopolymerization |
| CN107474617A (en) * | 2017-07-15 | 2017-12-15 | 佛山实瑞先导材料研究院（普通合伙） | A kind of aqueous, environmental protective antibacterial antifouling paint |
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| CN114042482A (en) | 2022-02-15 |
| CN112973821A (en) | 2021-06-18 |
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