CN103991165B - A kind of PCR plate injection molding forming method - Google Patents
A kind of PCR plate injection molding forming method Download PDFInfo
- Publication number
- CN103991165B CN103991165B CN201410252511.2A CN201410252511A CN103991165B CN 103991165 B CN103991165 B CN 103991165B CN 201410252511 A CN201410252511 A CN 201410252511A CN 103991165 B CN103991165 B CN 103991165B
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- China
- Prior art keywords
- die cavity
- pcr plate
- reaction tube
- pcr
- connecting plate
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C45/00—Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mould; Apparatus therefor
- B29C45/14—Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mould; Apparatus therefor incorporating preformed parts or layers, e.g. injection moulding around inserts or for coating articles
- B29C45/14598—Coating tubular articles
- B29C45/14614—Joining tubular articles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C45/00—Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mould; Apparatus therefor
- B29C45/17—Component parts, details or accessories; Auxiliary operations
- B29C45/1701—Component parts, details or accessories; Auxiliary operations using a particular environment during moulding, e.g. moisture-free or dust-free
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C45/00—Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mould; Apparatus therefor
- B29C45/17—Component parts, details or accessories; Auxiliary operations
- B29C45/26—Moulds
- B29C45/32—Moulds having several axially spaced mould cavities, i.e. for making several separated articles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C45/00—Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mould; Apparatus therefor
- B29C45/17—Component parts, details or accessories; Auxiliary operations
- B29C45/26—Moulds
- B29C45/34—Moulds having venting means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C45/00—Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mould; Apparatus therefor
- B29C45/14—Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mould; Apparatus therefor incorporating preformed parts or layers, e.g. injection moulding around inserts or for coating articles
- B29C2045/1486—Details, accessories and auxiliary operations
- B29C2045/14967—Injecting through an opening of the insert
Landscapes
- Engineering & Computer Science (AREA)
- Manufacturing & Machinery (AREA)
- Mechanical Engineering (AREA)
- Injection Moulding Of Plastics Or The Like (AREA)
- Moulds For Moulding Plastics Or The Like (AREA)
Abstract
The present invention relates to PCR plate injection molding technology field, particularly relate to a kind of PCR plate injection molding forming method, comprise the following steps: A, preparation PCR plate frame；B, in mould formed PCR plate die cavity, make PCR plate die cavity have PCR plate frame-type chamber, connecting plate die cavity, multiple reaction tube die cavity, and prepared PCR plate frame be placed in PCR plate frame-type chamber；C, the plastic material of molten condition is injected in PCR plate die cavity by connecting plate die cavity form PCR plate, the mouth of a river of the PCR plate prepared is positioned at the inner side of connecting plate, and on reaction tube, there is no the mouth of a river, improve product surface quality and performance, reaction tube smooth surface is neat, dimensionally stable, translucent effect is good, it is possible to the example reaction situation in observing response pipe well, improves Product checking effect, and glue-feeder is fewer needed for mould, simplifying mould structure, production efficiency is high, and water port of product is also few, production cost is low, conservation.
Description
Technical field:
The present invention relates to PCR plate injection molding technology field, particularly relate to a kind of PCR plate
Injection molding forming method.
Background technology:
At present, during molecular biology research and medical clinic applications, PCR reacts
Technology is indispensable.PCR reaction i.e. polymerase chain reaction (polymerase chain
Reaction, PCR), it is a kind of specific gene of rapid amplifying in vitro or the side of DNA sequence
Method, therefore it is also called the amplification in vitro method of gene.DNA segment both sides to be amplified and with
Two oligonucleotide primers that its both sides are complementary, through degeneration, anneal and extend several circulations
After, DNA cloning 2 square time times.In order to batch expands, PCR reaction is commonly used
PCR Sptting plate, as shown in accompanying drawing 1～3, PCR Sptting plate includes connecting plate 1 ' with many
Individual reaction tube 2 ', PCR Sptting plate is usually 8 holes, 12 holes, 96 holes or 384 holes
, i.e. corresponding reaction tube 2 ' is 8,12,96 or 384, be exclusively for
Batch reaction design, for PCR instrument and sequenator.
Existing PCR Sptting plate when injection mo(u)lding, typically PCR Sptting plate is inverted into
Row injection mo(u)lding, injection forming mold includes the lower mold that upper mold matches with upper mold, under
Mould is arranged on upper mold lower surface, forms PCR plate die cavity, PCR plate between upper mold and lower mold
Die cavity has connecting plate die cavity 5 ' and multiple reaction tubes being upside down in connecting plate die cavity 5 ' upper end
Die cavity 6 ', when entering glue, is typically all and is respectively provided with at the top of each reaction tube die cavity 6 '
One glue-feeder 7 ', injects raw material to PCR plate die cavity by each glue-feeder 7 ' simultaneously,
But the mouth of a river can be formed with bottom each reaction tube of the PCR plate so produced, instead
Should pipe lower surface rough, irregular, size is unstable, and affect light penetrates effect,
The example reaction situation being unfavorable in observing response pipe, affects Detection results, and is molded into
The glue-feeder that mould needs is the most, and mould structure is complicated, and production efficiency is low, produces into
This height, wastes raw material.
Summary of the invention:
The purpose of the present invention is aiming at the deficiency of prior art existence and provides one to carry
High product surface quality and performance, the PCR plate injection molding forming method of raising injection efficiency.
To achieve these goals, the technical solution used in the present invention is:
A kind of PCR plate injection molding forming method, comprises the following steps:
A, preparation PCR plate frame, make PCR plate frame form multiple hole position corresponding with reaction tube；
B, in mould formed PCR plate die cavity, make PCR plate die cavity have and PCR
PCR plate frame-type chamber, the connecting plate die cavity of sheet frame coupling, multiple it is upside down on connecting plate die cavity
The reaction tube die cavity of end, multiple reaction tube die cavities and position, multiple hole one_to_one corresponding, and will prepare
PCR plate frame be placed in PCR plate frame-type chamber；
C, the plastic material of molten condition is injected in PCR plate die cavity by connecting plate die cavity,
Plastic material is made to be full of connecting plate die cavity and reaction tube die cavity and be combined into one with PCR plate frame
Form PCR plate.
PCR plate frame in step A is formed by PC material injection.
Plastic material in step C is PP material.
Before carrying out described step C, produce negative inspiratory pressure shape first with air compression system
State, by the top of each reaction tube die cavity by the air sucking-off in PCR plate die cavity, makes PCR
Template intracavity forms negative pressure.
The negative pressure line connected with extraneous air compressibility is offered in advance in mould,
The negative pressure line top respectively with each reaction tube die cavity is made to connect, will by negative pressure line
Air sucking-off in PCR plate die cavity.
In described step C, in mould, offer multiple glue-feeder in advance, make each enter
Jiao Kou connects with connecting plate die cavity respectively, is noted to PCR plate die cavity by each glue-feeder simultaneously
Enter plastic material.
The plurality of reaction tube die cavity is divided into multiple groups, often each reaction tube die cavity in group it
Between connected by connecting plate die cavity, each connecting plate die cavity is corresponding with a glue-feeder even
Logical.
The plurality of reaction tube die cavity is matrix distribution, and every 4 adjacent reaction tube die cavities are
One group,
Described connecting plate die cavity is positioned at the upper end in PCR plate frame-type chamber.
The present invention has the beneficial effects that: the present invention comprises the following steps: A, preparation PCR plate
Frame, makes PCR plate frame form multiple hole position corresponding with reaction tube；B, in mould
Formed PCR plate die cavity, make PCR plate die cavity have PCR plate frame-type chamber, connecting plate die cavity,
Multiple reaction tube die cavities, and prepared PCR plate frame is placed in PCR plate frame-type chamber；C、
The plastic material of molten condition is injected in PCR plate die cavity by connecting plate die cavity, makes plastic cement former
Material is full of connecting plate die cavity and reaction tube die cavity and is combined into one formation PCR with PCR plate frame
Plate, the mouth of a river of prepared PCR plate is positioned at the inner side of connecting plate, and does not has the mouth of a river on reaction tube,
Improving product surface quality and performance, reaction tube smooth surface is neat, dimensionally stable, printing opacity
Effective, it is possible to the example reaction situation in observing response pipe well, improve Product checking
Needed for effect, and mould, glue-feeder is fewer, simplifies mould structure, and production efficiency is high,
Water port of product is also few, and production cost is low, conservation.
Accompanying drawing illustrates:
Fig. 1 is the structural representation of existing PCR plate.
Fig. 2 is the injection mo(u)lding schematic diagram of existing PCR plate.
Fig. 3 is the glue-feeder distribution schematic diagram of existing PCR plate.
Fig. 4 is the structural representation of injection forming mold of the present invention.
Fig. 5 is the structural representation of PCR plate die cavity of the present invention.
Fig. 6 is the injection mo(u)lding schematic diagram of PCR plate of the present invention.
Fig. 7 is the glue-feeder distribution schematic diagram of the PCR plate utilizing the present invention to prepare.
Fig. 8 is the structural representation of the PCR plate utilizing the present invention to prepare.
Detailed description of the invention:
Below in conjunction with the accompanying drawings and preferred embodiment the invention will be further described, see Fig. 4～8
Shown in, PCR plate includes PCR plate frame 1, multiple connecting plate 2 and multiple reaction tube 3,
Multiple reaction tubes 3 are in matrix distribution, and every 4 adjacent reaction tubes 3 are one group, each group
In 4 reaction tubes 3 between connected by a connecting plate 2, PCR plate frame 1 is by PC
Material is made, and connecting plate 2 and reaction tube 3 are made up of PP material.Certain all of reaction tube
3 can also only be connected by a connecting plate 2.
The injection molding forming method of above-mentioned PCR plate, comprises the following steps:
One, preparation PCR plate frame 1, makes PCR plate frame 1 be formed multiple corresponding with reaction tube 3
Position, hole.
Two, in mould 4 formed PCR plate die cavity, make PCR plate die cavity have with
PCR plate frame 1 coupling PCR plate frame-type chamber 41, be positioned at upper end, PCR plate frame-type chamber 41
Connecting plate die cavity 42, multiple reaction tube die cavity 43 being upside down in connecting plate die cavity 42 upper end,
Multiple reaction tube die cavities 43 connect with position, multiple hole one_to_one corresponding, and by prepared PCR plate
Frame 1 is placed in PCR plate frame-type chamber 41；Certainly, connecting plate die cavity 42 can also be positioned at PCR
Sheet frame die cavity 41 lower end.
Three, air compression system is utilized to produce negative inspiratory pressure state, by each reaction tube die cavity
The top of 43, by the air sucking-off in PCR plate die cavity, forms negative pressure in making PCR plate die cavity.
Wherein, in mould 4, connect with extraneous air compressibility negative is offered in advance
Pressure pipe road 5, makes the negative pressure line 5 top respectively with each reaction tube die cavity 43 connect, logical
Cross negative pressure line 5 by the air sucking-off in PCR plate die cavity.
Four, the plastic material of molten condition is injected PCR plate by connecting plate die cavity 42 upper end
In die cavity, the reaction tube die cavity 43 under negative pressure state can be by raw material by connecting plate die cavity 42
Middle attraction ascends in reaction tube die cavity 43, makes plastic material be rapidly filled with connecting plate die cavity
42 and reaction tube die cavity 43 being combined into one formation PCR plate with PCR plate frame 1, injection
Efficiency is high, and avoids oxygen and plastic material generation reaction in PCR plate die cavity to make plastic cement former
Material burn, improves the performance of plastic cement products and surface quality, it is possible to injection molding is more accurate,
The less product with thin wall type of size；It addition, the present invention also is able to utilize air compression system
Malleation blowing method by product blow out, prevent product to be bonded in upper mold, prevent deformation of products and
Damage, the demoulding is easy.
Wherein, in mould 4, multiple glue-feeder 6, each glue-feeder 6 are offered in advance
It is respectively distributed in the space between adjacent multiple reaction tube die cavity 43, makes each glue-feeder 6
Bottom connect with connecting plate die cavity 42 upper end respectively, by each glue-feeder 6 simultaneously to
PCR plate die cavity injects plastic material.Multiple reaction tube die cavities 43 are divided into multiple groups, often in group
Each reaction tube die cavity 43 between connected by a connecting plate die cavity 42, each connection
Template chamber 42 correspondence connects with a glue-feeder 6, by each glue-feeder 6 simultaneously to PCR
Plastic material is injected in template chamber, conveniently enters glue simultaneously.In the present embodiment, multiple reaction tubes
Die cavity 43 is in matrix distribution, and every 4 adjacent reaction tube die cavities 43 are one group.
PCR plate frame 1 is formed by PC material injection, connecting plate 2 and the plastic cement of reaction tube 3
Raw material is PP material so that prepared PCR plate has good stability, and heat conductivility is excellent,
Repeatedly heat indeformable advantage.
The mouth of a river of PCR plate utilizing the inventive method to prepare is positioned at the inner side of connecting plate 2, and
There is no the mouth of a river on reaction tube 3, improve product surface quality and performance, reaction tube 3 surface light
Sliding neat, dimensionally stable, translucent effect is good, it is possible to the sample in observing response pipe 3 well
Product response situation, improves Product checking effect, and needed for mould, glue-feeder 6 is fewer,
Simplifying mould structure, production efficiency is high, and water port of product is also few, and production cost is low, saves former
Material.
Certainly, the above is only presently preferred embodiments of the present invention, therefore all according to patent of the present invention
The equivalence that structure, feature and principle described in application range is done changes or modifies, and all includes
In the range of present patent application.
Claims (5)
1. a PCR plate injection molding forming method, it is characterised in that comprise the following steps:
A, preparation PCR plate frame, make PCR plate frame form multiple hole position corresponding with reaction tube；
B, in mould formed PCR plate die cavity, make the connecting plate die cavity that PCR plate die cavity has the PCR plate frame-type chamber mated with PCR plate frame, is positioned at upper end, PCR plate frame-type chamber, multiple reaction tube die cavity being upside down in connecting plate die cavity upper end, multiple reaction tube die cavities and position, multiple hole one_to_one corresponding, and prepared PCR plate frame is placed in PCR plate frame-type chamber；
C, produce negative inspiratory pressure state first with air compression system, by the top of each reaction tube die cavity by the air sucking-off in PCR plate die cavity, make to be formed in PCR plate die cavity negative pressure；
The plastic material of molten condition is injected in PCR plate die cavity by connecting plate die cavity, makes plastic material be full of connecting plate die cavity and reaction tube die cavity and be combined into one formation PCR plate with PCR plate frame；
Wherein, in mould, offer multiple glue-feeder in advance, make each glue-feeder connect with connecting plate die cavity respectively, inject plastic material to PCR plate die cavity by each glue-feeder simultaneously；Multiple reaction tube die cavities are divided into multiple groups, are often connected by a connecting plate die cavity between each reaction tube die cavity in group, and each connecting plate die cavity correspondence connects with a glue-feeder.
A kind of PCR plate injection molding forming method the most according to claim 1, it is characterised in that: the PCR plate frame in step A is formed by PC material injection.
A kind of PCR plate injection molding forming method the most according to claim 1, it is characterised in that: the plastic material in step C is PP material.
A kind of PCR plate injection molding forming method the most according to claim 1, it is characterized in that: in mould, offer the negative pressure line connected with extraneous air compressibility in advance, the negative pressure line top respectively with each reaction tube die cavity is made to connect, by negative pressure line by the air sucking-off in PCR plate die cavity.
A kind of PCR plate injection molding forming method the most according to claim 1, it is characterised in that: the plurality of reaction tube die cavity is matrix distribution, and every 4 adjacent reaction tube die cavities are one group
。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410252511.2A CN103991165B (en) | 2014-06-09 | 2014-06-09 | A kind of PCR plate injection molding forming method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410252511.2A CN103991165B (en) | 2014-06-09 | 2014-06-09 | A kind of PCR plate injection molding forming method |
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| Publication Number | Publication Date |
|---|---|
| CN103991165A CN103991165A (en) | 2014-08-20 |
| CN103991165B true CN103991165B (en) | 2016-08-24 |
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| CN201410252511.2A Active CN103991165B (en) | 2014-06-09 | 2014-06-09 | A kind of PCR plate injection molding forming method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3188885B1 (en) * | 2014-09-04 | 2018-05-09 | Basf Se | Method of manufacturing a multilayered composite part with integrated reinforcing structure |
| GB2561356B (en) * | 2017-04-10 | 2021-02-24 | Abgene Ltd | Overflow moulding apparatus and method |
| CN117384448A (en) * | 2023-12-12 | 2024-01-12 | 苏州亚通生物医疗科技有限公司 | PCR plate and preparation method thereof |
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| US7347977B2 (en) * | 2000-06-08 | 2008-03-25 | Eppendorf Ag | Microtitration plate |
| WO2007029101A2 (en) * | 2005-09-06 | 2007-03-15 | Bioinnovations Oy | Vessel and method of manufacture thereof |
| CN202878613U (en) * | 2012-10-26 | 2013-04-17 | 金发科技股份有限公司 | Mold and molded product |
| CN103302813B (en) * | 2013-06-20 | 2016-03-09 | 东莞市积健生物科技有限公司 | Plastic cement products negative pressure injection forming mold and method thereof |
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| CN103991165A (en) | 2014-08-20 |
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Effective date of registration: 20151008 Address after: 523000 Guangdong city of Dongguan province Shijie Zhen Shui Nan Chen Wu Cun industrial area C C02 Applicant after: DONGGUAN JIJIAN BIOTECHNOLOGY Co.,Ltd. Address before: 523000, Guangdong, Dongguan province Shijie Town Road, Chung Chen Industrial Zone bell Sheng mold factory Applicant before: Zhong Canqiu |
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Address after: No. 49, Chonghuan East Road, Shijie Town, Dongguan City, Guangdong Province, 523000 Patentee after: Guangdong Jijian Biotechnology Co.,Ltd. Address before: C02, Industrial Zone C, Chenwu Village, Shuinan, Shijie Town, Dongguan City, Guangdong Province, 523000 Patentee before: DONGGUAN JIJIAN BIOTECHNOLOGY Co.,Ltd. |