CN103947524A - Breeding method for inducing adventitious buds and roots by cutting petioles of metabriggsia ovalifolia - Google Patents
Breeding method for inducing adventitious buds and roots by cutting petioles of metabriggsia ovalifolia Download PDFInfo
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- CN103947524A CN103947524A CN201410168332.0A CN201410168332A CN103947524A CN 103947524 A CN103947524 A CN 103947524A CN 201410168332 A CN201410168332 A CN 201410168332A CN 103947524 A CN103947524 A CN 103947524A
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Abstract
The invention discloses a breeding method for inducing adventitious buds and roots by cutting petioles of metabriggsia ovalifolia. The breeding method comprises the following steps: from March to April every year, taking the petiole with leaves and the length being 2-10cm of the metabriggsia ovalifolia as an explant; soaking the explant in a plant-growth regulator for 30-60 minutes, then cutting the explant in matrix, or firstly cutting the explant in the matrix and then spraying the plant growth regulator on the explant; adopting a sun-shading, ventilating and air-permeable greenhouse as the cutting growth environment of the explant, controlling the ambient temperature to be 18-25 DEG C and the relative humidity to be 70-95%, and watering for maintaining the humidity till adventitious buds and adventitious roots grow. The breeding method disclosed by the invention has the advantages that rare and endangered plants can be better protected and utilized, a set of simple-fast-effective breeding system and plant regeneration system is established, the national first batch of first-level species are effectively protected and bred for eliminating the endangered state, so that good foundation is laid for further protecting and utilizing the species.
Description
Technical field:
The invention belongs to breeding plant technical field, be specifically related to the mating system of a kind of single seat lettuce tongue petiole cuttage evoking adventive bud and root.
Background technology:
Single seat lettuce tongue (Metabriggsia ovalifolia W.T.Wang) belongs to perennial small-sized herbaceous plant for Gesneriaceae single seat lettuce tongue.For country-level focused protection wild plant and Endemic Plants From Guangxi.Have important view and admire, medical value and scientific research conservation value.But due to single seat lettuce tongue distributed number and limited, habitat is special, and population quantity is few, natural propagation power is weak, its vegetation fragmentation, and population quantity and distribution area sharply reduce, and its fundamental biological knowledge research is almost blank.
The technology of breeding the aspect of taking root with the petiole cuttage of single seat lettuce tongue, is not reported both at home and abroad.
Summary of the invention:
The object of this invention is to provide the mating system of a kind of single seat lettuce tongue petiole cuttage evoking adventive bud and root, utilize this method to obtain indefinite bud and adventive root by cutting propagation, again through transplanting, in short time, can obtain large quantities of seedlings, and then grow up to plant, thereby for better protecting and utilize the rare or endangered species of first emphasis first class of protection of this China, set up a set of simple, fast, effectively breeding system and plant regeneration system, effectively protect and breed first one-level species of this country, make it to remove Critical Condition, for further protecting from now on and utilize these species to lay a good foundation.
The mating system of single seat lettuce tongue petiole cuttage evoking adventive bud of the present invention and root, is characterized in that, comprises the following steps:
In the annual 3-4 month, the long petiole with blade of 2-10cm of getting single seat lettuce tongue (Metabriggsia ovalifolia W.T.Wang) is explant; Explant is soaked in to 30～60min in plant growth regulator, and then cuttage is in matrix, or first by explant cuttage in matrix, and then spray plant growth regulator on explant; The cuttage growing environment of explant is that in sunshade, ventilation, ventilative green house, the temperature that controls environment is 18～25 DEG C, and relative moisture is 70～95%, and the moisturizing of watering, until grow indefinite bud and adventive root;
Described matrix is the mixture of perlite or river sand or both arbitrary proportions;
Described plant growth regulator is: 6-benzyl purine (BA) 1～5mg/L, indole-3-butyric acid (IBA) 1～5mg/L, heteroauxin (IAA) (1-5mg L
-1) or methyl α-naphthyl acetate (NAA) (1-5mg L
-1).
Described is according to each 10ml/ explant toward spraying plant growth regulator on explant again, in 7 days, on explant, sprays plant growth regulator 2 times.
In general three months, just can induce multiple new plant by a petiole explant.
The present invention is by selecting suitable explant, then through plant growth regulator treatment, cuttage, in suitable matrix, is cultivated, and induced indefinite bud and adventive root under suitable condition, transplants and grows up to seedling and then grow up to plant through plant division.The present invention can be simple and convenient, effective fast, breeds at short notice large quantities of seedlings, and then grow up to plant.Thereby for better protecting and utilize these rare or endangered species; set up a set of system and plant regeneration system of breeding simply, fast and effectively; effectively protect and breed first one-level species of this country; make it to remove Critical Condition, for further protecting from now on and utilize these species to lay a good foundation.
Embodiment:
Following examples are to further illustrate of the present invention, instead of limitation of the present invention.
Embodiment 1:
In March, get the long petiole with blade of the primulina tabacum 2.0cm that grows up as explant, by explant first with 1.0mg L
-1iBA aqueous solution soaking was processed after 30 minutes, the oblique cuttage of this petiole squint is entered in river sand matrix, blade stands on matrix face, cuttage growing environment is that in sunshade, ventilation on all sides, ventilative green house, the temperature that controls environment is between 18～25 DEG C, and relative moisture is controlled between 70～95%, be separated by and water with moisturizing for two days, cultivate after 50 days, root survival is 85%, and indefinite bud mean is 3.3.By be transplanted in the mixed-matrix of peat soil and loess (1:1, v/v) cellar culture in strong sprout with the bud of adventive root, until grow up to plant.
Embodiment 2:
In March, get the long petiole with blade of the primulina tabacum 2.0cm that grows up as explant, by explant first with 5.0mgL
-1iBA aqueous solution soaking was processed after 30 minutes, the oblique cuttage of this petiole squint is entered in river sand matrix, blade stands on matrix face, cuttage growing environment is that in sunshade, ventilation on all sides, ventilative green house, the temperature that controls environment is between 18～25 DEG C, and relative moisture is controlled between 70～95%, be separated by and water with moisturizing for two days, cultivate after 50 days, root survival is 80%, and indefinite bud mean is 4.0.By be transplanted in the mixed-matrix of peat soil and loess (1:1, v/v) cellar culture in strong sprout with the bud of adventive root, until grow up to plant.
Embodiment 3:
In March, get the long petiole with blade of the primulina tabacum 3.0cm that grows up as explant, first by explant cuttage in matrix (river sand and perlite by volume 1:1 mix), blade stands on matrix face, and then toward the each explant sprinkling 1.0mg L in matrix
-1iAA10ml, sprays in the week 2 times altogether.Cuttage growing environment is that in sunshade, ventilation on all sides, ventilative green house, the temperature that controls environment is between 18～25 DEG C, and relative moisture is controlled between 70～95%, be separated by and water with moisturizing for two days, cultivate after 55 days, root survival is 91.7%, and indefinite bud mean is 6.7.By be transplanted in the mixed-matrix of peat soil and loess (1:1, v/v) cellar culture in strong sprout with the bud of adventive root, until grow up to plant.
Embodiment 4:
In March, get the long petiole with blade of the primulina tabacum 5.0cm that grows up as explant, by explant first with 5.0mgL
-1iAA aqueous solution soaking was processed after 60 minutes, the oblique cuttage of this petiole squint is entered in the matrix that river sand and perlite mix according to volume ratio 1:1, blade stands on matrix face, cuttage growing environment is that in sunshade, ventilation on all sides, ventilative green house, the temperature that controls environment is between 18～25 DEG C, and relative moisture is controlled between 70～95%, be separated by and water with moisturizing for two days, cultivate after 60 days, root survival is 73.3%, and the blade indefinite bud mean of survival is 3.3.By be transplanted in the mixed-matrix of peat soil and loess (1:1, v/v) cellar culture in strong sprout with the bud of adventive root, until grow up to plant.
Embodiment 5:
In April, get the long petiole with blade of the primulina tabacum 5.0cm that grows up as explant, by explant first with 1.0mg L
-1bA aqueous solution soaking was processed after 60 minutes, the oblique cuttage of this petiole squint is entered in the matrix of river sand, blade stands on matrix face, cuttage growing environment is that in sunshade, ventilation on all sides, ventilative green house, the temperature that controls environment is between 18～25 DEG C, and relative moisture is controlled between 70～95%, be separated by and water with moisturizing for two days, cultivate after 60 days, root survival is 56.7%, and the blade indefinite bud mean of survival is 3.3.By be transplanted in the mixed-matrix of peat soil and loess (1:1, v/v) cellar culture in strong sprout with the bud of adventive root, until grow up to plant.
Embodiment 6:
In April, get the long petiole with blade of the primulina tabacum 5.0cm that grows up as explant, by explant first with 5.0mg L
-1bA aqueous solution soaking was processed after 60 minutes, the oblique cuttage of this petiole squint is entered in the matrix that river sand and perlite mix according to volume ratio 1:1, blade stands on matrix face, cuttage growing environment is that in sunshade, ventilation on all sides, ventilative green house, the temperature that controls environment is between 18～25 DEG C, and relative moisture is controlled between 70～95%, be separated by and water with moisturizing for two days, cultivate after 60 days, root survival is 53.3%, and the blade indefinite bud mean of survival is 5.0.By be transplanted in the mixed-matrix of peat soil and loess (1:1, v/v) cellar culture in strong sprout with the bud of adventive root, until grow up to plant.
Embodiment 7:
In April, get the long petiole with blade of the primulina tabacum 3.0cm that grows up as explant, by explant first with 1.0mg L
-1nAA aqueous solution soaking was processed after 30 minutes, the oblique cuttage of this petiole squint is entered in the matrix of river sand, blade stands on matrix face, cuttage growing environment is that in sunshade, ventilation on all sides, ventilative green house, the temperature that controls environment is between 18～25 DEG C, and relative moisture is controlled between 70～95%, be separated by and water with moisturizing for two days, cultivate after 50 days, root survival is 96%, and the blade indefinite bud mean of survival is 4.7.By be transplanted in the mixed-matrix of peat soil and loess (1:1, v/v) cellar culture in strong sprout with the bud of adventive root, until grow up to plant.
Embodiment 8:
In April, get the long petiole with blade of the primulina tabacum 10.0cm that grows up as explant, by explant first with 5.0mg L
-1nAA aqueous solution soaking was processed after 30 minutes, the oblique cuttage of this petiole squint is entered in the matrix of river sand, blade stands on matrix face, cuttage growing environment is that in sunshade, ventilation on all sides, ventilative green house, the temperature that controls environment is between 18～25 DEG C, and relative moisture is controlled between 70～95%, be separated by and water with moisturizing for two days, cultivate after 50 days, root survival is 91%, and the blade indefinite bud mean of survival is 3.3.By be transplanted in the mixed-matrix of peat soil and loess (1:1, v/v) cellar culture in strong sprout with the bud of adventive root, until grow up to plant.
Comparative example:
In March, get the long petiole with blade of the primulina tabacum 4.0cm that grows up as explant, by explant first with running water immersion treatment after 30 minutes, the oblique cuttage of this petiole squint is entered in the matrix of river sand, blade stands on matrix face, cuttage growing environment is in sunshade, ventilation on all sides, ventilative green house, the temperature that controls environment is between 18～25 DEG C, relative moisture is controlled between 70～95%, be separated by and water with moisturizing for two days, cultivate after 50 days, root survival is 38.3%, indefinite bud mean is 0, finally can not form regrowth.
Claims (2)
1. a mating system for single seat lettuce tongue petiole cuttage evoking adventive bud and root, is characterized in that, comprises the following steps:
In the annual 3-4 month, the long petiole with blade of 2-10cm of getting single seat lettuce tongue (Metabriggsia ovalifolia W.T.Wang) is explant; Explant is soaked in to 30～60min in plant growth regulator, and then cuttage is in matrix, or first by explant cuttage in matrix, and then spray plant growth regulator on explant; The cuttage growing environment of explant is that in sunshade, ventilation, ventilative green house, the temperature that controls environment is 18～25 DEG C, and relative moisture is 70～95%, and the moisturizing of watering, until grow indefinite bud and adventive root;
Described matrix is the mixture of perlite or river sand or both arbitrary proportions;
Described plant growth regulator is: 6-benzyl purine 1～5mg/L, indole-3-butyric acid 1～5mg/L, heteroauxin 1-5mg L
-1or methyl α-naphthyl acetate 1-5mg L
-1.
2. mating system according to claim 1, is characterized in that, described is according to each 10ml/ explant toward spraying plant growth regulator on explant again, in 7 days, on explant, sprays plant growth regulator 2 times.
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|---|---|---|---|
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104430306A (en) * | 2014-11-10 | 2015-03-25 | 中国科学院昆明植物研究所 | Gesneriaceae plant cryopreservation method |
| CN106550762A (en) * | 2016-12-07 | 2017-04-05 | 贵州省林业科学研究院 | A kind of Libo lip post lettuce tongue cuttage breeding method |
| CN109005966A (en) * | 2018-07-10 | 2018-12-18 | 深圳市仙湖植物园管理处（深圳市园林研究中心） | A kind of cuttage breeding method of Gesneriaceae |
| CN115386591A (en) * | 2022-09-05 | 2022-11-25 | 中国科学院华南植物园 | Molecular breeding method of monochantia sonchifolia |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101965800A (en) * | 2010-11-19 | 2011-02-09 | 中国科学院华南植物园 | Tissue culture breeding method for metabriggsia ovalifolia |
| CN102405755A (en) * | 2011-09-13 | 2012-04-11 | 中国科学院华南植物园 | Method for breeding adventitious buds and adventitious roots induced by primula denticulata petiole cutting |
-
2014
- 2014-04-24 CN CN201410168332.0A patent/CN103947524A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101965800A (en) * | 2010-11-19 | 2011-02-09 | 中国科学院华南植物园 | Tissue culture breeding method for metabriggsia ovalifolia |
| CN102405755A (en) * | 2011-09-13 | 2012-04-11 | 中国科学院华南植物园 | Method for breeding adventitious buds and adventitious roots induced by primula denticulata petiole cutting |
Non-Patent Citations (1)
| Title |
|---|
| 艾春晓等: "《4种报春苣苔属植物叶插繁殖技术研究》", 《广东农业科学》, no. 6, 25 March 2013 (2013-03-25), pages 43 - 46 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104430306A (en) * | 2014-11-10 | 2015-03-25 | 中国科学院昆明植物研究所 | Gesneriaceae plant cryopreservation method |
| CN106550762A (en) * | 2016-12-07 | 2017-04-05 | 贵州省林业科学研究院 | A kind of Libo lip post lettuce tongue cuttage breeding method |
| CN109005966A (en) * | 2018-07-10 | 2018-12-18 | 深圳市仙湖植物园管理处（深圳市园林研究中心） | A kind of cuttage breeding method of Gesneriaceae |
| CN115386591A (en) * | 2022-09-05 | 2022-11-25 | 中国科学院华南植物园 | Molecular breeding method of monochantia sonchifolia |
| CN115386591B (en) * | 2022-09-05 | 2024-05-28 | 中国科学院华南植物园 | Molecular breeding method of single herba Cichorii |
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Application publication date: 20140730 |