CN103875923A - Fat coated biological enzyme or micro-ecological preparation - Google Patents
Fat coated biological enzyme or micro-ecological preparation Download PDFInfo
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- CN103875923A CN103875923A CN201410134978.7A CN201410134978A CN103875923A CN 103875923 A CN103875923 A CN 103875923A CN 201410134978 A CN201410134978 A CN 201410134978A CN 103875923 A CN103875923 A CN 103875923A
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Abstract
The invention discloses a fat coated biological enzyme or micro-ecological preparation. A preparation method of the fat coated biological enzyme or micro-ecological preparation comprises the steps of firstly, compressing solvent liquefied petroleum gas and the like which are in a gas state under the conditions of normal pressure and room temperature to obtain liquid, and storing the liquid into a storage tank; putting a coating material into a dissolving kettle, vacuumizing, injecting the liquid solvent in the storage tank into the dissolving kettle by a hydrocarbon pump, and heating the liquid solvent until the liquid solvent becomes subcritical fluid; stirring to dissolve the coating material; feeding an enzyme preparation or micro-ecological preparation into a packaging kettle, sealing and then vacuumizing; opening the hydrocarbon pump and injecting the solution containing the coating material in the dissolving kettle into the packaging kettle; reducing the pressure for releasing the solvent; controlling the temperature of the material in the packaging kettle not to exceed 35 DEG C; when the pressure in the packaging kettle becomes normal pressure, vacuumizing and emptying the packaging kettle to enable the pressure in the packaging kettle to become normal pressure; and discharging to obtain the coated enzyme preparation or micro-ecological preparation finished product. The preparation method has the advantages that the whole process can be completed under the conditions of being close to room temperature and free of oxygen, so that the inactivation of the enzyme preparation or the micro-ecological preparation can be greatly alleviated in the processing process, and the product activity is improved.
Description
Technical field
The present invention relates to feed addictive---biology enzyme and probiotics, especially relate to a kind of biology enzyme and probiotics of fatty coating.
Background technology
Enzyme is the protein with catalytic activity, has high efficiency, selectivity, is extensively present in animals and plants and microbial body, is that organism maintains the requisite composition of normal physiological biochemical function.Poultry, domestic animal is absorbed after various macromolecular substances being degraded to small-molecule substance under the effect of various enzymes in alimentary canal again.In animal feed, the abilities of digestive and absorption of nutritional labeling depends on kind and the active size of enzyme in alimentary canal.But the endogenous enzymes of the degraded feed ingredient of secreting in livestock and poultry alimentary tract often lacks and deficiency, as the phytase of the phytic acid of degrading, the cellulase of degraded cellulose and other some non-starch polysaccharide enzymes all relatively lack, this just greatly reduces the digestive utilization ratio of nutriment, thereby need in feed, add exogenous enzymes preparation, to improve feed quality, improve efficiency of feed utilization.Along with the development of modern biotechnology; the cost of fodder enzyme preparation is more and more lower; but in use heat treatment; there is again very large impact to the stability of enzyme preparation in moisture and some metal ion etc.: phytase, amylase etc. are expanded middle active can obviously reduction of granulating; the activity of dry enzyme preparation is better; generally can tolerate 90 DEG C of high temperature 30 minutes and can passivation inactivation; but the same temperature of the damp and hot generation of steam but can cause the rapid inactivation of enzyme preparation; in the time that granulator refining temperature reaches 75 DEG C, the activity of beta glucan only has 30% of initial activity.
Probiotics is a kind of prebiotics feed additive that by improving gut flora balance, animal is applied the work of Beneficial Effect, is active bacteria formulation that can be directly feeding, and for improving, livestock and poultry production performance is significant.The domestic confirmed suitable bacterial classification that does probio has 12 kinds, Bacillus acidi lactici, streptococcus, bacillus, Bifidobacterium and saccharomycete etc. at present.Probiotics as feed addictive problem demanding prompt solution is: 1. active bacteria formulation easily loses biologically active in feed processing, transport, storage process, these microorganisms are especially responsive to high temperature, and in the time that pelleting temperature exceedes 85 DEG C, activity is almost lost Tai to the greatest extent; 2. active bacteria formulation enters after alimentary canal, can not stand the hydrochloric acid of low pH, and the effect of bile acid is difficult to have enough number of viable to arrive enteron aisle or surely grows play a role in enteron aisle that (General Requirements probiotics viable bacteria concentration is 10
7cfu/mL); 3. after active bacteria formulation enters enteron aisle, the speed of growth is slow, be difficult to the status of having the advantage in microorganism competition, form dominant microflora, therefore, although some microorganism formulation has good effect under laboratory condition, under working condition, be often difficult to get a desired effect.
For the feature of above-mentioned biology enzyme and probiotics, people adopt microcapsules film-coating technique that biology enzyme and probiotics are converted into stable fine-powdered particle, make it have good mobility and dispersiveness, be easy to mix with other feeds, and convenient transport, storage and interpolation; Protection through the product of coating due to membrane material, acidproof and resistance to elevated temperatures significantly improves.
At present, method for coating enzyme preparation (and probiotics) is a lot, spray drying process and spray cooling are conventional means, be wall material as Liu Hanling adopts beta-schardinger dextrin-, the papain microcapsules that spray-dried method makes, after measured, microcapsule formulation heat endurance in the time of 75 DEG C improves more than 2 times, and in feed steam pelletization, the activity of 1,4 beta-glucanase can retain more than 90%; Yuan Jieli etc. utilize Arabic gum to carry out coating to Bifidobacterium, make the Bifidobacterium death rate reduce by 20% ~ 30%.But it is higher that spray-dired shortcoming is hot blast temperature, in processing, loss of activity is large, processing cost is also high simultaneously, preparing microcapsules by chemical method can carry out at lower temperature, as peptostreptococcus and Bacillus acidi lactici carried out to coating with sodium alginate and calcium chloride, significant prolongation the survival period of Bacillus acidi lactici, but preparation feedback carries out in the aqueous solution, product drying needs higher temperature equally; Because said method is all to make in air atmosphere, airborne oxygen can cause the death of anaerobe (as Bifidobacterium).
Summary of the invention
The object of the present invention is to provide that a kind of preparation method is simple, loss of activity is few and have biology enzyme and a probiotics significantly antiacid, resistant to elevated temperatures fatty coating in process.
For achieving the above object, the present invention can take following technical proposals:
The biology enzyme of fatty coating of the present invention and probiotics are to be prepared from by following step:
The first step, by the solvent liquefied petroleum gas (LPG), normal butane, high-purity iso-butane (R600a), propane, the dimethyl ether (DME), 1 that under normal temperature and pressure are gaseous state, 1,1,2-HFC-134a or sulfur hexafluoride through compressor boil down to fluid storage in storage tank;
Second step, coating material is placed in to the dissolution kettle of jacketed, being evacuated to gauge pressure is 0.09MPa, and the liquid flux in above-mentioned storage tank is entered in dissolution kettle by hydrocarbon infusion, in chuck, logical hot water heats up liquid flux becomes subcritical fluids, stirs coating material is dissolved;
The 3rd step, jacketed seal still in add enzyme preparation or probiotics, after sealing, being evacuated to gauge pressure is 0.09MPa, driving hydrocarbon pump injects the solution containing coating material in dissolution kettle to seal still, stir 20 ~ 30 minutes, decompression release solvent, phase transformation institute calorific requirement provides by sealing hot water in still chuck, hot water temperature is that the material temperature that 0 ~ 80 DEG C of control is sealed in still is no more than 35 DEG C, in the time sealing still internal pressure to normal pressure, being evacuated to gauge pressure is 0.09MPa, then emptying makes to seal still internal pressure to normal pressure, discharging, obtain coating enzyme preparation or probiotics finished product,
The 4th step, collects the gas-solvent discharging in the 3rd step, and this gas-solvent is again converted into fluid storage and enters storage tank after purified treatment.
Above-mentioned the 3rd step is also replaceable is:
Jacketed seal still in add enzyme preparation or probiotics, after sealing, being evacuated to gauge pressure is 0.09MPa, drives hydrocarbon pump by injecting and sealing still containing the solution of coating material in dissolution kettle, stirs 20 ~ 30 minutes; To seal material in still and introduce spray tower through high-pressure pump, atomizer by spray tower sprays, fluid solvent gasification immediately after atomizer decompression discharges, and coating material adheres to the probiotics finished product of enzyme preparation or probiotics surface formation coating enzyme preparation or coating.Coordinate mist projection granulating, subcritical fluids solvent, through shower nozzle ejection step-down immediately gasification, absorbs a large amount of heat energy simultaneously, makes coating enzyme preparation and probiotics cooling rapidly, has reduced the height of spray tower, saves energy, and particle is finer and smoother evenly simultaneously.
Described coating material is the mixture of hydrogenated vegetable oil, palm stearin, higher fatty acids polyol polyester and above-mentioned material.
Because above-mentioned coating material is hard fat, for helping fat to dissolve in enteron aisle, coating material can also comprise 1 ~ 20% glycerin monostearate or/and polyglycerol fatty acid ester or/and stearic acid calcium lactate.
The granularity of described coating enzyme preparation or probiotics finished product is 0.02 ~ 0.5mm.
In second step, the mixing temperature of dissolution kettle is 0 ~ 35 DEG C (optimum temperature is 10 ~ 30 DEG C), and pressure is 0.1 ~ 0.8MPa.
The invention has the advantages that coating material subcritical fluids is dissolved into the solution containing coating material, by solvent dilution coating material, then mix and carry out coating with enzyme preparation and probiotics, its advantage may be embodied in the following aspects: 1. whole process can complete under the condition that approaches room temperature, greatly reduce enzyme preparation and probiotics inactivation in process, improved the activity of product.2. process is carried out under oxygen free condition, is especially applicable to the coating of the anaerobic bacterias such as Bifidobacterium.3. coating material adopts the composite of hard fat and polyester, can resistance to feed processing in the processing of wet-hot steam.4. membrane wrapping thickness is adjustable, and product can evenly discharge in enteron aisle.
Brief description of the drawings
Fig. 1 is the process chart of embodiment 1.
Fig. 2 is the process chart of embodiment 2,3.
Detailed description of the invention
Further illustrate the preparation method of biology enzyme and the probiotics of fatty coating below by specific embodiment.
Embodiment 1:
Coating material adopts stearic acid glycerine succinic acid polyester (89 DEG C of fusing points) 60%, hydrogenated vegetable oil (50 DEG C of fusing points) 30%, and the percentage by weight of polyglycerol fatty acid ester 10% is prepared.
As shown in Figure 1, contain agitator or supersonic generator at the dissolution kettle 1(of jacketed, pressure-bearing P >=2MPa) in add 350kg to prepare according to the above ratio coating material, sealing, being evacuated to dissolution kettle vacuum meter registration is that gauge pressure is 0.09MPa, drives hydrocarbon pump 3 and injects 2000L liquefied petroleum gas (or other solvents) (being stored in storage tank 4 after compressing in advance, be cooled to liquid), stirs, logical hot water hydrotropy in chuck, contains the solution temperature of coating material lower than 35 DEG C in dissolution kettle 1.
By 150kg cellulase, (enzyme lives 1 × 10
4u/g) be placed in and seal still (adopting rake type dryer 2), the solution that is dissolved with coating material in dissolution kettle 1 is squeezed in rake type dryer to 2 by hydrocarbon pump 5 to be mixed 30 minutes, open air valve 6 desolventizing (gas-solvent of release becomes liquid through compressor 7, condenser 8 after purifying, and to return to storage tank 4 interior for subsequent use) that reduces pressure, material is emitted collection from drain hole, obtain 30% coating cellulase finished product, envelop rate 96.5%.When coated product makees pellet, through 20 minutes non-inactivations of 80 DEG C of humid heat treatment.After entering in animal body as feed, can tolerate the hydrochloric acid of low pH, the effect of bile acid, ensure that the vigor of cellulase plays a role in enteron aisle.
Embodiment 2:
Coating material adopts palm stearin 50%, stearic acid glycerine adipate polyester 40%, and the percentage by weight of polyglycerol fatty acid ester 10% is prepared.
As shown in Figure 2, contain agitator or supersonic generator at dissolution kettle 1(, pressure-bearing P >=2MPa) in add 200kg to prepare according to the above ratio coating material, sealing, being evacuated to dissolution kettle vacuum meter registration is that gauge pressure is 0.09MPa, drives hydrocarbon pump 3 and injects 1000L butane (or other solvents) (being stored in storage tank 4 after compressing in advance, be cooled to liquid), stir, chuck leads to hot water hydrotropy, and in dissolution kettle 1, solution temperature is lower than 35 DEG C, and in still to be dissolved, feed liquid becomes after transparent and stops hot water supply.
In still 2, add 300kg lipase (enzyme lives 1 × 10 sealing
5u/g), being evacuated to and sealing still 2 vacuum meter registrations is 0.09MPa, drives hydrocarbon pump 5 feed liquid in dissolution kettle 1 is squeezed into and sealed in still 2, is uniformly mixed (30 minutes); Drive high-pressure pump 6 and will seal material introducing spray tower 7 in still 2, atomizer 8 by spray tower 7 sprays, the gasification immediately after atomizer 8 decompressions of fluid solvent butane discharges, open air valve 9, after the gas-solvent discharging purifies, become liquid through compressor 10, condenser 11 and return in storage tank 4 and reuse, collect the coating lipase finished product of content 60%.Envelop rate 92.1%, particle diameter 0.1 ~ 0.4mm, circle, good fluidity.When coated product makees pellet, through 20 minutes non-inactivations of 80 DEG C of humid heat treatment.After entering in animal body as feed, can tolerate the hydrochloric acid of low pH, the effect of bile acid, ensure that the vigor of lipase plays a role in enteron aisle.
Embodiment 3:
Coating material adopts hydrogenated vegetable oil 80%, single stearic acid glycerine lipoprotein 10%, and the percentage by weight of sucrose ester 10% is prepared.
As shown in Figure 2, contain agitator or supersonic generator at dissolution kettle 1(, pressure-bearing P >=2MPa) in add 100kg to be prepared by aforementioned proportion coating material, sealing, being evacuated to dissolution kettle vacuum meter registration is that gauge pressure is 0.09MPa, drive hydrocarbon pump 3 and inject 1000L butane (or other solvents) solution (being stored in storage tank 4 after compressing in advance, be cooled to liquid), stir, chuck leads to hot water hydrotropy, in dissolution kettle 1 containing the solution temperature of coating material lower than 35 DEG C, become after transparent and stop hot water supply until feed liquid in still.
In still 2, add 400kg Bifidobacterium-lactic acid bacteria solid powder (number of viable 2 × 10 sealing
8individual/g), being evacuated to and sealing still vacuum meter registration is that gauge pressure is 0.09MPa, drives hydrocarbon pump 5 feed liquid in dissolution kettle 1 is squeezed into and sealed in still 2, is uniformly mixed (30 minutes).Drive high-pressure pump 6 and will seal material introducing spray tower 7 in still 2, atomizer 8 by spray tower 7 sprays, the gasification immediately after atomizer decompression of fluid solvent butane discharges, open air valve 9, after the gas-solvent of release purifies, become liquid through compressor 10, condenser 11 and return in storage tank 4 and reuse; Collect the coating Bifidobacterium finished product of content 80%.Envelop rate 91.5%, particle diameter 0.1 ~ 0.4mm, circle, good fluidity.Coated product, 80 DEG C of humid heat treatment, is processed 20 minutes, and number of viable is preserved more than 90%.
Claims (6)
1. the biology enzyme of fatty coating and a probiotics, is characterized in that: be to be prepared from by following step:
The first step, by under normal temperature and pressure, be solvent liquefied petroleum gas, normal butane, high-purity iso-butane, propane, dimethyl ether, HFA 134a or the sulfur hexafluoride of gaseous state through compressor boil down to fluid storage in storage tank;
Second step, coating material is placed in to the dissolution kettle of jacketed, being evacuated to gauge pressure is 0.09MPa, and the liquid flux in above-mentioned storage tank is entered in dissolution kettle by hydrocarbon infusion, in chuck, logical hot water heats up liquid flux becomes subcritical fluids, stirs coating material is dissolved;
The 3rd step, jacketed seal still in add solid enzyme preparation or probiotics, after sealing, being evacuated to gauge pressure is 0.09MPa, driving hydrocarbon pump injects the solution containing coating material in dissolution kettle to seal still, stir 20 ~ 30 minutes, decompression release solvent, phase transformation institute calorific requirement provides by sealing hot water in still chuck, hot water temperature is 0 ~ 80 DEG C, control is sealed material temperature in still and is no more than 35 DEG C, in the time sealing still internal pressure and approach normal pressure, being evacuated to gauge pressure is 0.09MPa, then emptying makes to seal still internal pressure to normal pressure, discharging, obtain coating enzyme preparation or probiotics finished product,
The 4th step, collects the gas-solvent discharging in the 3rd step, and this gas-solvent enters storage tank through compressing the cooling fluid storage that is again converted into after purified treatment.
2. the biology enzyme of fatty coating according to claim 1 and probiotics, is characterized in that: above-mentioned the 3rd step is replaceable is:
Can stir at jacketed seal still in add enzyme preparation or probiotics, after sealing, being evacuated to gauge pressure is 0.09MPa, drives hydrocarbon pump by injecting and sealing still containing the solution of coating material in dissolution kettle, stirs 20 ~ 30 minutes; To seal material in still and introduce spray tower through high-pressure pump, atomizer by spray tower sprays, fluid solvent gasification immediately after atomizer decompression discharges, and coating material adheres to enzyme preparation or probiotics surface forms coating enzyme preparation or probiotics finished product.
3. the biology enzyme of fatty coating according to claim 1 and probiotics, is characterized in that: described coating material is the mixture of hydrogenated vegetable oil, palm stearin, higher fatty acids polyol polyester and above-mentioned material.
4. the biology enzyme of fatty coating according to claim 3 and probiotics, is characterized in that: described coating material also comprise 1 ~ 20% glycerin monostearate or/and sucrose ester or/and polyglycerol fatty acid ester or/and stearic acid calcium lactate.
5. the biology enzyme of fatty coating according to claim 1 and probiotics, is characterized in that: the granularity of described coating enzyme preparation or probiotics finished product is 0.02 ~ 0.5mm.
6. the biology enzyme of fatty coating according to claim 1 and probiotics, is characterized in that: in described second step, the mixing temperature of dissolution kettle is 0 ~ 35 DEG C, and pressure is 0.1 ~ 0.8MPa.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109566719A (en) * | 2018-12-28 | 2019-04-05 | 华南农业大学 | A method of fresh-keeping meat is assisted using subcritical state solvent |
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| CN109566719A (en) * | 2018-12-28 | 2019-04-05 | 华南农业大学 | A method of fresh-keeping meat is assisted using subcritical state solvent |
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