CN103820492A - Functional nano catechin gene-introduction material and preparation method thereof - Google Patents
Functional nano catechin gene-introduction material and preparation method thereof Download PDFInfo
- Publication number
- CN103820492A CN103820492A CN201310520826.6A CN201310520826A CN103820492A CN 103820492 A CN103820492 A CN 103820492A CN 201310520826 A CN201310520826 A CN 201310520826A CN 103820492 A CN103820492 A CN 103820492A
- Authority
- CN
- China
- Prior art keywords
- catechin
- gene
- preparation
- ferrous sulfate
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 title claims abstract description 43
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 title claims abstract description 43
- 235000005487 catechin Nutrition 0.000 title claims abstract description 43
- 229950001002 cianidanol Drugs 0.000 title claims abstract description 43
- 239000000463 material Substances 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims abstract description 22
- 235000003891 ferrous sulphate Nutrition 0.000 claims abstract description 14
- 239000011790 ferrous sulphate Substances 0.000 claims abstract description 14
- 108090000623 proteins and genes Proteins 0.000 claims description 23
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 239000012153 distilled water Substances 0.000 claims description 14
- 238000012546 transfer Methods 0.000 claims description 13
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 239000008367 deionised water Substances 0.000 claims description 8
- 229910021641 deionized water Inorganic materials 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 7
- 238000001476 gene delivery Methods 0.000 claims description 5
- 108010043121 Green Fluorescent Proteins Proteins 0.000 claims description 4
- 102000004144 Green Fluorescent Proteins Human genes 0.000 claims description 4
- 238000012637 gene transfection Methods 0.000 claims description 4
- 239000005090 green fluorescent protein Substances 0.000 claims description 4
- 239000006210 lotion Substances 0.000 claims description 4
- 239000013612 plasmid Substances 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000000967 suction filtration Methods 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 238000001890 transfection Methods 0.000 abstract description 20
- 230000004083 survival effect Effects 0.000 abstract description 8
- 206010006187 Breast cancer Diseases 0.000 abstract description 4
- 208000026310 Breast neoplasm Diseases 0.000 abstract description 4
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 abstract description 4
- 229910001448 ferrous ion Inorganic materials 0.000 abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 3
- 239000004480 active ingredient Substances 0.000 abstract description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 abstract 2
- 241001122767 Theaceae Species 0.000 abstract 1
- 201000008275 breast carcinoma Diseases 0.000 abstract 1
- 150000008442 polyphenolic compounds Chemical class 0.000 abstract 1
- 230000001988 toxicity Effects 0.000 abstract 1
- 231100000419 toxicity Toxicity 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 15
- 239000000243 solution Substances 0.000 description 11
- 239000012097 Lipofectamine 2000 Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 241000700605 Viruses Species 0.000 description 3
- 239000002105 nanoparticle Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 230000003612 virological effect Effects 0.000 description 3
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 230000010165 autogamy Effects 0.000 description 2
- 229910000389 calcium phosphate Inorganic materials 0.000 description 2
- 239000001506 calcium phosphate Substances 0.000 description 2
- 235000011010 calcium phosphates Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000002502 liposome Substances 0.000 description 2
- 238000000520 microinjection Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 241000702421 Dependoparvovirus Species 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 102000052575 Proto-Oncogene Human genes 0.000 description 1
- 108700020978 Proto-Oncogene Proteins 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000003855 balanced salt solution Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 238000000975 co-precipitation Methods 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000012268 genome sequencing Methods 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- ICIWUVCWSCSTAQ-UHFFFAOYSA-M iodate Chemical compound [O-]I(=O)=O ICIWUVCWSCSTAQ-UHFFFAOYSA-M 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000003584 silencer Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 239000012096 transfection reagent Substances 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
Images
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a functional nano catechin gene-introduction material. Catechin and ferrous sulfate are combined into a catechin-Fe<2+> nanometer spherical material of which the average diameter is about 100 nm, the catechin content is 0.94 g/g, and the ferrous ion content is 0.06 g/g. The invention further discloses a preparation method of the functional nano catechin gene-introduction material. The functional nano catechin gene-introduction material has relatively high transfection efficiency which can reach about 40 percent in human breast carcinoma cells. The toxicity is low, the catechin is high in biocompatibility, and thus the cell survival rate is very high. The dispersity of the material is high and the material conforms to the requirements of transfection; the cost is extremely low; the catechin is a main active ingredient extracted from tea leaves, namely a polyphenolic compound, the price of the catechin is low, and ferrous sulfate is also a common cheap reagent which is easy to get. For the material preparation, the reaction is simple and easy in operation, and the repeatability is high.
Description
[technical field]
The present invention relates to gene transfer material of the functionalized nano catechin of a kind of high transfection efficiency, low cytotoxicity and preparation method thereof.
[background technology]
Along with completing that Human Genome Sequencing is measured, we have obtained breakthrough progress to the pathogenetic understanding of human diseases on gene level.At present research shows, has the generation of numerous disease and development and gene closely related.If can examination go out and the special relevant gene fragment of disease or transgenation, just can on gene level, carry out targetedly specific therapy, as be correlated with by importing missing gene or silencer, to strengthen relevant disappearance function or reticent Disease-causing gene, thereby reach the object of thorough treatment.It is key and the difficult point in current this research field that goal gene is imported in organism safely and effectively.
Gene delivery system or method can be divided into two classes: virus type Gene delivery system, take retrovirus, adenovirus, adeno-associated virus as carrier; Non-viral type gene imports, as microinjection, particle gun, coprecipitation of calcium phosphate, cationic-liposome method and emerging nanometer gene transfer material.Because virus type Gene delivery system exists many serious deficiencies, as virus transfection likely activates proto-oncogene etc., therefore non-viral type rotaring transfecting mode is current study hotspot, in above-mentioned non-viral formula transfection, microinjection once can only be processed a cell; Particle gun penetration power is very limited; Calcium phosphate precipitation unstable result; Only cationic-liposome method has showed good transfection efficiency, but too high toxicity has limited again its application.
[summary of the invention]
The object of the present invention is to provide gene transfer material of a kind of functionalized nano catechin and preparation method thereof, the good biocompatibility of its gene transfer material, and the matrix material being formed by the material of low toxicity, it is to belong to non-viral type carrier, show good cell compatibility, stable and there is higher transfection efficiency.
To achieve these goals, the gene transfer material technical scheme of functionalized nano catechin of the present invention is: a kind of gene transfer material of functionalized nano catechin, it is that catechin, ferrous sulfate are combined into catechin-Fe that mean diameter is 100nm left and right
2+nanometer spherical material, catechin content is 0.94g/g, ferrous ion content is 0.06g/g.Catechin and ferrous ion coordination, be self-assembled into catechin-Fe
2+mixture.Due to the positive polarity of its surperficial ferrous ion, make it possess the potentiality that become transfection material.Catechin can be controlled the amorphous catechin-Fe of generation
2+for spherical pattern, can control the particle diameter of nano particle by the control in reaction times.
Described catechin-Fe
2+nanometer spherical material energy and green fluorescent protein plasmid gene pGFP, with non-covalent mode combination, form inorganic nano Gene delivery system, for gene transfection.
The method of preparing functionalized nano catechin gene transfer material comprises the steps:
(1) draw materials: ferrous sulfate, analytical pure; Catechin, MW660, analytical pure, when use without being further purified step, all preparation glasswares, all in ethanol ultrasonic 5 minutes, then distilled water cleaned, and uses washing lotion H
20/HNO
3(65%)/H
2o
2(35%) (1:1:1, v/v/v) cleans, and cleans successively more afterwards with distilled water, acetone, finally in air, dries.
(2) preparation of material: prepare the copperas solution of 2mM (0.02mmol/L) new system with deionized water dissolving ferrous sulfate, prepare 2mM(0.02mmol/L by deionized water dissolving catechin) catechin solution of new system; First, get 2mL2mM(0.02mmol/L) add the catechin solution of 2ml2mM new system in copperas solution, then add 16ml distilled water, and be placed in the beaker of 25mL, after fully stirring, hold over night, suction filtration is collected.
The present invention has advantages of following:
1, there is higher transfection efficiency, in human breast cancer cell, can reach 40% left and right.
2, hypotoxicity, catechin has good biocompatibility, and cells survival rate is very high.
3, material scatter is good, meets the requirement to transfection.
4, cost is extremely low, and catechin is the main active ingredient of extracting in tealeaves---polyphenolic compound, cheap; The pharmaceutical chemicals using in experiment in addition, if ferrous sulfate etc. is all the common cheap reagent being easy to get; Material preparation feedback is simple to operation, favorable repeatability.
[accompanying drawing explanation]
Fig. 1 is catechin-Fe
2+scanning electron microscope picture.
Fig. 2 is fluorescence picture.
Fig. 3 is the inversion micro mirror after transfection.
Fig. 4 is the cells survival rate picture after transfection.
Fig. 5 is transfection efficiency figure.
[embodiment]
One, the preparation of gene transfer material of the present invention:
1, draw materials: main raw: ferrous sulfate, analytical pure, Aldrich company product; Catechin, MW660, analytical pure, when use without being further purified step, Aldrich company product.All preparation glasswares, all in ethanol ultrasonic 5 minutes, then distilled water cleaned, and uses washing lotion H
20/HNO
3(65%)/H
2o
2(35%) (1:1:1, v/v/v) cleans, and cleans successively more afterwards with distilled water, acetone, finally in air, dries.
2, the preparation of material: prepare 2mM(0.02mmol/L with deionized water dissolving ferrous sulfate) copperas solution of new system, prepare 2mM(0.02mmol/L by deionized water dissolving catechin) catechin solution of new system; First, get 2mL2mM(0.02mmol/L) add the catechin solution of 2ml2mM new system in copperas solution, then add 16ml distilled water, and be placed in the beaker of 25mL, after fully stirring, hold over night, suction filtration is collected.Consult Fig. 1,2 and be respectively catechin-Fe
2+scanning electron microscope sem and transmission electron microscope TEM figure.
Two, the mensuration of bonding properties
1, main raw
Green fluorescent protein plasmid (pEGFP-C1); HEPES balanced salt solution (autogamy); Electrophoretic buffer (0.5 × TBE, autogamy); DNA sample-loading buffer; Ethidum Eremide (EB).
2, main method
1 μ L catechin-Fe
2+solution (5mg catechin-Fe
2+be dissolved in 500 μ L distilled waters) mix in 1.5mL centrifuge tube with the 1 μ LpEGFP-C1 aqueous solution (0.1 μ g/ μ L) and 8 μ l distilled waters (pH=7.4water), be placed in room temperature and within lower 30 minutes, allow its abundant combination.Catechin-Fe
2+use respectively 100:1,50:1,30:1,10:1,1:1 with the mass ratio of pEGFP-C1.Centrifugal 5min under the speed of 5000rpm.Throw out is loaded into 1% agarose (EB0.1 μ g/mL) and, under the buffering of TAE, under 100V voltage, runs 40min, then observe band at 320nm place.
3, result
Result is observed, and has multiple band to occur, this is because pEGFP-C1 has due to multiple configuration.At mass ratio 30:1,10:1,1:1 place band is clear obviously to be illustrated under these mass ratioes, and DNA and nano particle, in conjunction with being not fine, have arrived 100:1, and 50:1 band disappears, and description taken in conjunction is complete.
These results show: positively charged pEGFP-C1 and catechin-Fe
2+have a best combination ratio, exceed 50:1 later too much nano particle seem and recede into the background, so use 50:1 to carry out transfection.
Three, transfection
1, main raw
Human breast cancer cell; Catechin-Fe
2+@pEGFP-C1 association (above-mentioned preparation); DMEM substratum; Foetal calf serum FBS; 6 well culture plates.
2, main method
(1) cultivation of cell: human breast cancer cell is digested with trypsinase-EDTA, join in 6 orifice plates (5 × 10 after counting
6/ hole), with containing the DMEM solution of FBS10% and add transfection and optimize reagent to be cultured to cell density be 60%～70% degrees of fusion.
(2) cell transfecting experiment: cultured cell conditioned medium is removed, and the nutrient solution renewing also adds catechin-Fe of mass ratio 50:1
2+@pEGFP-C1 association, cultivates after 48 hours and respectively its fluorescence (consulting Fig. 3), cell survival rate (iodate pyridylamination, flow cytometer detects) (consulting Fig. 4), transfection efficiency (flow cytometer detection) is measured.(consulting Fig. 5)
3, interpretation of result
Fluorogram can be found out obvious green fluorescence, and coverage rate is larger.Cells survival rate figure is respectively without additive, catechin-Fe
2+the survival rate of@pEGFP-C1 association, lipofectamine2000@pEGFP-C1 association, can see catechin-Fe
2+@pEGFP-C1 association is very little on the impact of cells survival rate.Little more a lot of than the impact of lipofectamine2000@pEGFP-C1 association.Transfection efficiency figure can see catechin-Fe
2+@pEGFP-C1 association can reach approximately 40% transfection efficiency, and this is to be issued to so transfection efficiency in the prerequisite that has guaranteed cells survival rate.Although high not as good as lipofectamine2000, its high cell compatibility is that lipofectamine2000 is incomparable.Show catechin-Fe
2+as the great potential of transfection reagent.
Claims (4)
1. a gene transfer material for functionalized nano catechin, is characterized in that: it is that catechin, ferrous sulfate are combined into catechin-Fe that mean diameter is 100nm left and right
2+nanometer spherical material, catechin content is 0.94g/g, ferrous sulfate content is 0.06g/g; It is to make according to following method: draw materials (1): ferrous sulfate, analytical pure; Catechin, MW660, analytical pure, when use without being further purified step, all preparation glasswares, all in ethanol ultrasonic 5 minutes, then distilled water cleaned, and uses washing lotion H
20/HNO
3(65%)/H
2o
2(35%) (1:1:1, v/v/v) cleans, and cleans successively more afterwards with distilled water, acetone, finally in air, dries; (2) preparation of material: prepare 2mM(0.02mmol/L with deionized water dissolving ferrous sulfate) copperas solution of new system, prepare 2mM(0.02mmol/L by deionized water dissolving catechin) catechin solution of new system; First, get 2mL2mM(0.02mmol/L) add the catechin solution of 2ml2mM new system in copperas solution, then add 16ml distilled water, and be placed in the beaker of 25mL, after fully stirring, hold over night, suction filtration is collected.
2. the gene transfer material of functionalized nano catechin according to claim 1 is for the purposes of gene transfection; it is characterized by its energy and green fluorescent protein plasmid gene pGFP with non-covalent mode combination; form inorganic nano Gene delivery system, for gene transfection.
3. the gene transfer material of functionalized nano catechin according to claim 2, for the purposes of gene transfection, is characterized by green fluorescent protein plasmid and catechin-Fe
2+the combination of nanometer spherical material the best is than being 50:1.
4. a preparation method for the gene transfer material of functionalized nano catechin, is characterized in that, it comprises the steps:
(1) draw materials: ferrous sulfate, analytical pure; Catechin, MW660, analytical pure, when use without being further purified step, all preparation glasswares, all in ethanol ultrasonic 5 minutes, then distilled water cleaned, and uses washing lotion H
20/HNO
3(65%)/H
2o
2(35%) (1:1:1, v/v/v) cleans, and cleans successively more afterwards with distilled water, acetone, finally in air, dries;
(2) preparation of material: prepare 2mM(0.02mmol/L with deionized water dissolving ferrous sulfate) copperas solution of new system, prepare 2mM(0.02mmol/L by deionized water dissolving catechin) catechin solution of new system; First, get 2mL2mM(0.02mmol/L) add the catechin solution of 2ml2mM new system in copperas solution, then add 16ml distilled water, and be placed in the beaker of 25mL, after fully stirring, hold over night, suction filtration is collected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310520826.6A CN103820492A (en) | 2013-10-29 | 2013-10-29 | Functional nano catechin gene-introduction material and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310520826.6A CN103820492A (en) | 2013-10-29 | 2013-10-29 | Functional nano catechin gene-introduction material and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103820492A true CN103820492A (en) | 2014-05-28 |
Family
ID=50755781
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310520826.6A Pending CN103820492A (en) | 2013-10-29 | 2013-10-29 | Functional nano catechin gene-introduction material and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103820492A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105505962A (en) * | 2015-12-21 | 2016-04-20 | 中山大学附属第一医院 | Functionalized nano complex gene transfer material and preparation method and application thereof |
| WO2019072055A1 (en) * | 2017-10-09 | 2019-04-18 | 上海长征医院 | Composition for use in gene therapy or transfection, preparation method therefor and use thereof |
| CN109806252A (en) * | 2019-01-29 | 2019-05-28 | 中国药科大学 | Tri compound nanometer system and its preparation method and application |
| CN116003368A (en) * | 2022-12-21 | 2023-04-25 | 中国农业科学院茶叶研究所 | Catechin nanosphere reversible self-assembly material, preparation method and biological application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101148680A (en) * | 2007-10-23 | 2008-03-26 | 南京凯瑞尔纳米生物技术有限公司 | Method for leading plasmid carrier containing gene cure segment in cell by nano particles |
| CN101299998A (en) * | 2005-08-19 | 2008-11-05 | 麦格霍斯奈米生技股份有限公司 | Method for encapsulating therapeutic substances in cells |
| WO2009023697A3 (en) * | 2007-08-14 | 2009-04-02 | Univ California | Hollow silica nanospheres and methods of making same |
| CN102202815A (en) * | 2008-05-16 | 2011-09-28 | 维鲁泰克技术股份有限公司 | Green synthesis of nanometals using plant extracts and use thereof |
| KR101171648B1 (en) * | 2010-12-31 | 2012-08-06 | 숭실대학교산학협력단 | Metal nanoparticle associated with chitosan or BPEI, RNAi, and Raman dye, and methods of analysing efficiency of its transfection into cell and identifying location of it in cell by using it |
| CN103212080A (en) * | 2012-01-21 | 2013-07-24 | 京华堂实业股份有限公司 | Nano composition having cancer prevention and treatment effects and preparation method |
-
2013
- 2013-10-29 CN CN201310520826.6A patent/CN103820492A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101299998A (en) * | 2005-08-19 | 2008-11-05 | 麦格霍斯奈米生技股份有限公司 | Method for encapsulating therapeutic substances in cells |
| WO2009023697A3 (en) * | 2007-08-14 | 2009-04-02 | Univ California | Hollow silica nanospheres and methods of making same |
| CN101148680A (en) * | 2007-10-23 | 2008-03-26 | 南京凯瑞尔纳米生物技术有限公司 | Method for leading plasmid carrier containing gene cure segment in cell by nano particles |
| CN102202815A (en) * | 2008-05-16 | 2011-09-28 | 维鲁泰克技术股份有限公司 | Green synthesis of nanometals using plant extracts and use thereof |
| KR101171648B1 (en) * | 2010-12-31 | 2012-08-06 | 숭실대학교산학협력단 | Metal nanoparticle associated with chitosan or BPEI, RNAi, and Raman dye, and methods of analysing efficiency of its transfection into cell and identifying location of it in cell by using it |
| CN103212080A (en) * | 2012-01-21 | 2013-07-24 | 京华堂实业股份有限公司 | Nano composition having cancer prevention and treatment effects and preparation method |
Non-Patent Citations (4)
| Title |
|---|
| BIN ZHU 等: ""Hydrogen Bond Induced Exfoliation of Nanoparticles in Polymer Matrix and Its Application in Foaming Process with Supercritical Carbon Dioxide"", 《CHEMICAL JOURNAL OF CHINESE UNIVERSITIES》 * |
| M. H., FAIZAH 等: ""THE COMPLEXATION OF FE-CATECHIN FROM FERROUS AND FERRIC IONS AT DIFFERENT pH"", 《MALAYSIAN JOURNAL OF MEDICAL SCIENCES》 * |
| YS WU 等: ""Fluorescent Detection of Lead in Environmental Water and Urine Samples Using Enzyme Mimics of Catechin-Synthesized Au Nanoparticles"", 《APPLIED MATERIALS & INTERFACES》 * |
| 邓迎春 等: ""功能化Fe3O4的制备及在基因转染中的应用"", 《生物医学工程与临床》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105505962A (en) * | 2015-12-21 | 2016-04-20 | 中山大学附属第一医院 | Functionalized nano complex gene transfer material and preparation method and application thereof |
| CN105505962B (en) * | 2015-12-21 | 2018-08-31 | 中山大学附属第一医院 | Functionalized nano complex gene transfer material and preparation method and application thereof |
| WO2019072055A1 (en) * | 2017-10-09 | 2019-04-18 | 上海长征医院 | Composition for use in gene therapy or transfection, preparation method therefor and use thereof |
| CN109806252A (en) * | 2019-01-29 | 2019-05-28 | 中国药科大学 | Tri compound nanometer system and its preparation method and application |
| WO2020155673A1 (en) * | 2019-01-29 | 2020-08-06 | 中国药科大学 | Ternary complex nanometer system, preparation method therefor and use thereof |
| CN109806252B (en) * | 2019-01-29 | 2021-08-10 | 中国药科大学 | Ternary composite nano system and preparation method and application thereof |
| CN116003368A (en) * | 2022-12-21 | 2023-04-25 | 中国农业科学院茶叶研究所 | Catechin nanosphere reversible self-assembly material, preparation method and biological application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Izumrudov et al. | Interpolyelectrolyte complexes: Advances and prospects of application | |
| CN103641106B (en) | Preparation method of nano sulfonated graphene and application of nano sulfonated graphene as gene transfer material | |
| Rabiee et al. | Green synthesis of ZnO NPs via Salvia hispanica: Evaluation of potential antioxidant, antibacterial, mammalian cell viability, H1N1 influenza virus inhibition and photocatalytic activities | |
| Zhao et al. | Modification of nanostructured calcium carbonate for efficient gene delivery | |
| Wang et al. | Biomineralization-based virus shell-engineering: towards neutralization escape and tropism expansion | |
| CN103820492A (en) | Functional nano catechin gene-introduction material and preparation method thereof | |
| CN110615829B (en) | Self-assembled antibacterial peptide hydrogel | |
| Chu et al. | A multifunctional carbon dot-based nanoplatform for bioimaging and quaternary ammonium salt/photothermal synergistic antibacterial therapy | |
| CN106698447B (en) | Hollow mesoporous silica nanoparticle, nano-carrier and preparation method thereof | |
| Yan et al. | Green synthesis of carbon quantum dots from plant turmeric holds promise as novel photosensitizer for in vitro photodynamic antimicrobial activity | |
| Yin et al. | A multifunctional upconverting nanoparticle incorporated polycationic hydrogel for near-infrared triggered and synergistic treatment of drug-resistant bacteria | |
| CN101791408A (en) | Cationic polymer gene vector with low cytotoxicity and high transfection efficiency as well as preparation method and application thereof | |
| CN103305549A (en) | Genetic vector system of nanoparticle with multiple oxidation-reduction stimulus response as well as preparation method and application of genetic vector system | |
| Abutaha et al. | Rational design of Ag-ZnO-Fe3O4 nanocomposite with promising antimicrobial activity under LED light illumination | |
| Cheang et al. | Calcium carbonate/CaIP 6 nanocomposite particles as gene delivery vehicles for human vascular smooth muscle cells | |
| CN103555767B (en) | The preparation method of the microRNA nano-carrier of a kind of LBL self-assembly and application thereof | |
| CN106622162B (en) | A kind of preparation method and application of modification of chitosan/zeolite/nanometer zirconium adsorbent | |
| CN103990138B (en) | Layer-by-layer assembled nanogold composite drug delivery carrier system, preparation method and application thereof | |
| Deng et al. | Thermo-responsive polymer–black phosphorus nanocomposites for NIR-triggered bacterial capture and elimination | |
| CN103849650B (en) | The gene transfer material and preparation method of functionalized nano curcumin | |
| CN103820493A (en) | Nano heparin sodium-PEI-Ca<2+> gene-introduction material and preparation method | |
| CN101792772B (en) | Gene transfer material and preparation method | |
| CN103627719B (en) | Nano gene transfer material and preparation method and application thereof | |
| Nowacka et al. | Electrokinetic and bioactive properties of CuO∙ SiO2 oxide composites | |
| CN104109690A (en) | Functional nano gene transfer material and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20170824 Address after: No. two, No. 58, Zhongshan Road, Guangzhou, Guangdong, Yuexiu District Applicant after: The First Affiliated Hospital,Sun Yat-sen University Address before: 510080, No. two, No. 58, Zhongshan Road, Guangzhou, Guangdong, Yuexiu District Applicant before: Wang Shenming Applicant before: Xu Anwu Applicant before: Zhang Deyuan |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140528 |