CN103828715A - Bletilla striata tissue culture breeding method - Google Patents
Bletilla striata tissue culture breeding method Download PDFInfo
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- CN103828715A CN103828715A CN201310632169.4A CN201310632169A CN103828715A CN 103828715 A CN103828715 A CN 103828715A CN 201310632169 A CN201310632169 A CN 201310632169A CN 103828715 A CN103828715 A CN 103828715A
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Abstract
The invention discloses a bletilla striata tissue culture breeding method. The bletilla striata tissue culture breeding method comprises preparation of two groups of mediums, acquisition of an aseptic seedling, subculturing, seedling strengthening and rooting.
Description
Technical field
The present invention relates to tissue and breed field, is exactly the tissue culture method for breeding of a kind of bletilla striata.
Background technology
The bletilla striata, has another name called purple orchid, luxuriant tongue orchid, Lian Jicao, belongs to perennial herb bulbous plant (piece root), is mainly distributed in China, Taiwan, Japan and Upper Myanmar.Hua Mei is applicable to the use of viewing and admiring, and the name after its bulb dries is called bletilla (have another name called the bletilla striata, understand splendid achnatherum, purple blue root, Gan Gen, give free of charge), belongs to Chinese herbal medicine, and other also has the purposes of the thickener of being used as.The pattern of general kind is aubergine, so another name is purple blue; Separately there is the flower of a mutation for white, be called and spend the bletilla striata (B.s.var.alba) in vain.Other also has yellow, pink, redness and blue horticultural gardening kind.The main florescence is in spring, but according to the difference of various places weather, evening, Winter Solstice, early summer all may be bloomed.If plantation, because the easy sun blister of blade tip should be adopted the mode of irradiating for 1st, avoid with a western exposure summer as far as possible.
Summary of the invention
The object of the present invention is to provide the tissue culture method for breeding of a kind of bletilla striata.
Above-mentioned purpose realizes by following scheme:
A tissue culture method for breeding for the bletilla striata, is characterized in that: comprise the following steps:
(1) prepare two groups of medium:
First group is Initial stage of culture induction meristematic tissue and the synchronous medium of propagation (A): 1/2MS+6-BA1.0-2.0mg/L+NAA0.3-0.5mg/L, additional sugar 20-30g/L, agar 7.5g left and right/L, banana 80-100g/L, PH5.5-5.8;
Second group is late stage of culture strong sprout and synchronous medium (B): the MS+NAA1.0-2.0mg/L+IBA0.3-0.5mg/L of taking root, additional sugar 30g left and right/L, agar 7.5g left and right/L, banana 100g-120g/L, apple 20-25g/L, carrot 20-25g/L, PH5.5-5.8,24-28 DEG C of temperature;
(2) acquisition of aseptic seedling:
Taking wild bletilla striata capsule as explant, when capsule appearance has just transferred brown to, reach morphological maturity and gather while not yet ftractureing, bring use for laboratory detergent liquid into and clean 1min left and right, after rinsing is clean, in superclean bench with the HgCl of 0.1% left and right, sterilization 20-25min, aseptic water washing 3-4 times, each about 2min, subsequently the fruit pod after gained sterilizing is cut with blade, bletilla striata seeds is evenly sowed at medium (A) above, after about 70d, be divided into bletilla striata seedling;
(3) subculture is cultivated:
The bletilla striata seedling of above-mentioned acquisition is continued to switching upper at medium (A), 100d left and right can develop into the seedling that 1.0-1.5cm is high, every 40-50d subculture 1 time, and the seedling of must growing thickly;
(4) strong sprout and taking root:
The seedling switching of growing thickly that is less than 3cm is cultivated to circulation successively in the upper propagation that continues of medium (A); Proceed on medium (B) and carry out culture of rootage higher than the seedling of growing thickly of 3cm, 1 strain of every cave, after about 60d, as height of seedling 5-8cm, the long 4-6cm of root, can the cultivation of bottle outlet hardening.
The tissue culture method for breeding of described a kind of bletilla striata, is characterized in that: described subculture is cultivated can breed 2-6 times at every turn.
The tissue culture method for breeding of described a kind of bletilla striata, is characterized in that: the rooting rate of culture of rootage reaches 100%.
The tissue culture method for breeding of described a kind of bletilla striata, is characterized in that: bletilla striata seeds is evenly sowed after medium (A) is upper, cultivated 20-30d and can see that embryo obviously expands, become green from yellow.
Beneficial effect of the present invention is: the bletilla striata belongs to orchid, and seed is without endosperm, so natural germination rate is extremely low, natural propagation extreme difference, takes this world advanced person's tissue culture technology can make its germination rate reach more than 60%, has greatly increased the developmental potency of the bletilla striata.The germination of my the company original creation bletilla striata and prescription of rooting medium can effectively improve the natural germination rate of the bletilla striata and strengthen germination and the rootability of the bletilla striata.Therefore, this mating system plays very large facilitation and the Value Realization of rare traditional Chinese medicine is laid a good foundation the population protection of the orchid bletilla striata in imminent danger.
Brief description of the drawings
Fig. 1 is the seedling sowing of the bletilla striata;
Fig. 2 is the breeding of the bletilla striata;
Fig. 3 is the rooting process of the bletilla striata.
Embodiment
A tissue culture method for breeding for the bletilla striata, comprises the following steps:
(1) prepare two groups of medium:
First group is Initial stage of culture induction meristematic tissue and the synchronous medium of propagation (A): 1/2MS+6-BA1.0-2.0mg/L+NAA0.3-0.5mg/L, additional sugar 30g/L, agar 7.5g/L, banana 100g/L, PH5.5-5.8;
Second group is late stage of culture strong sprout and synchronous medium (B): the MS+NAA1.0-2.0mg/L+IBA0.3-0.5mg/L of taking root, additional sugar 30g left and right/L, agar 7.5g/L, banana 120g/L, apple 25g/L, carrot 25g/L, PH5.5-5.8,26 DEG C of temperature;
(2) acquisition of aseptic seedling:
Taking wild bletilla striata capsule as explant, when capsule appearance has just transferred brown to, when reaching morphological maturity and not yet ftractureing, gather, bring use for laboratory detergent liquid into and clean 1min, after rinsing is clean, in superclean bench with 0.1% HgCl, sterilization 25min, aseptic water washing 4 times, each 2min, cuts the fruit pod after gained sterilizing subsequently with blade, bletilla striata seeds is evenly sowed on medium (A), cultivate 30d and can see that embryo obviously expands, become green from yellow, after 70d, be divided into bletilla striata seedling;
(3) subculture is cultivated:
The bletilla striata seedling of above-mentioned acquisition is continued to switching upper at medium (A), 100d left and right can develop into the seedling that 1.5cm is high, every 50d subculture 1 time, and the seedling of must growing thickly;
(4) strong sprout and taking root:
The seedling switching of growing thickly that is less than 3cm is cultivated to circulation successively in the upper propagation that continues of medium (A); Proceed on medium (B) and carry out culture of rootage higher than the seedling of growing thickly of 3cm, 1 strain of every cave, after 60d, as height of seedling 8cm, the long 6cm of root, can the cultivation of bottle outlet hardening.
Described subculture is cultivated can breed 4 times at every turn.
The rooting rate of culture of rootage reaches 100%.
Claims (4)
1. a tissue culture method for breeding for the bletilla striata, is characterized in that: comprise the following steps:
(1) prepare two groups of medium:
First group is Initial stage of culture induction meristematic tissue and the synchronous medium of propagation (A): 1/2MS+6-BA1.0-2.0mg/L+NAA0.3-0.5mg/L, additional sugar 20-30g/L, agar 7.5g left and right/L, banana 80-100g/L, PH5.5-5.8;
Second group is late stage of culture strong sprout and synchronous medium (B): the MS+NAA1.0-2.0mg/L+IBA0.3-0.5mg/L of taking root, additional sugar 30g left and right/L, agar 7.5g left and right/L, banana 100g-120g/L, apple 20-25g/L, carrot 20-25g/L, PH5.5-5.8,24-28 DEG C of temperature;
(2) acquisition of aseptic seedling:
Taking wild bletilla striata capsule as explant, when capsule appearance has just transferred brown to, reach morphological maturity and gather while not yet ftractureing, bring use for laboratory detergent liquid into and clean 1min left and right, after rinsing is clean, in superclean bench with the HgCl of 0.1% left and right, sterilization 20-25min, aseptic water washing 3-4 times, each about 2min, subsequently the fruit pod after gained sterilizing is cut with blade, bletilla striata seeds is evenly sowed at medium (A) above, after about 70d, be divided into bletilla striata seedling;
(3) subculture is cultivated:
The bletilla striata seedling of above-mentioned acquisition is continued to switching upper at medium (A), 100d left and right can develop into the seedling that 1.0-1.5cm is high, every 40-50d subculture 1 time, and the seedling of must growing thickly;
(4) strong sprout and taking root:
The seedling switching of growing thickly that is less than 3cm is cultivated to circulation successively in the upper propagation that continues of medium (A); Proceed on medium (B) and carry out culture of rootage higher than the seedling of growing thickly of 3cm, 1 strain of every cave, after about 60d, as height of seedling 5-8cm, the long 4-6cm of root, can the cultivation of bottle outlet hardening.
2. the tissue culture method for breeding of a kind of bletilla striata according to claim 1, is characterized in that: described subculture is cultivated can breed 2-6 times at every turn.
3. the tissue culture method for breeding of a kind of bletilla striata according to claim 1, is characterized in that: the rooting rate of culture of rootage reaches 100%.
4. the tissue culture method for breeding of a kind of bletilla striata according to claim 1, is characterized in that: bletilla striata seeds is evenly sowed after medium (A) is upper, cultivated 20-30d and can see that embryo obviously expands, become green from yellow.
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104145816A (en) * | 2014-07-29 | 2014-11-19 | 郎溪县紫兰药材种植专业合作社 | Bletilla striata tissue-culture seedling raising method |
| CN104335903A (en) * | 2014-11-21 | 2015-02-11 | 广西中医药大学 | Method for accelerating quick propagation of rhizoma bletillae |
| CN104686346A (en) * | 2015-02-24 | 2015-06-10 | 陈桂容 | Tissue culture and rapid propagation method for divaricate saposhnikovia root |
| CN104770293A (en) * | 2015-04-01 | 2015-07-15 | 张子国 | Bletilla open-type tissue culture seedling method |
| CN104871977A (en) * | 2015-05-29 | 2015-09-02 | 江苏农林职业技术学院 | Bletilla striata tissue culture medium and its preparing method |
| CN104904597A (en) * | 2015-05-28 | 2015-09-16 | 云南省农业科学院农业环境资源研究所 | Bletilla striata rapid propagation and sunshine seedling strengthening method |
| CN104920208A (en) * | 2015-05-15 | 2015-09-23 | 浙江虹越花卉股份有限公司 | Culture medium for rapid propagation in tissue culture of bletilla striata |
| CN105145352A (en) * | 2015-08-03 | 2015-12-16 | 河南科技大学 | Efficient tissue culture and rapid propagation technology for seedlings of bletilla striata |
| CN105210875A (en) * | 2015-10-15 | 2016-01-06 | 铜仁市金农绿色农业科技有限公司 | A kind of bletilla striata quick propagation method |
| CN105766645A (en) * | 2016-03-31 | 2016-07-20 | 广西医科大学 | Efficient reproduction method of rhizoma bletillae tissue culture seedlings |
| CN106665349A (en) * | 2016-11-29 | 2017-05-17 | 重庆上古药业有限公司 | Planting method of bletilla |
| CN107047310A (en) * | 2017-05-10 | 2017-08-18 | 文山学院 | A kind of cultural method of bletilla striata seeds culture seedling |
| CN107173222A (en) * | 2017-03-27 | 2017-09-19 | 河池乐康生态农业科技有限公司 | A kind of wild bletilla striata quick breeding method for tissue culture |
| CN107466654A (en) * | 2017-09-12 | 2017-12-15 | 六盘水黔丰农业发展有限公司 | A kind of efficiently method of reproducing bletilla striata tissue-cultured seedling and the growth belt method of the bletilla striata |
| CN108055993A (en) * | 2017-12-01 | 2018-05-22 | 亳州学院 | The nutrient solution method that Dabie Mountain wild bletilla striata seedling stage sprouts |
| CN108094209A (en) * | 2017-12-28 | 2018-06-01 | 临沧市云瑞堂生物科技有限公司 | A kind of bletilla striata quickly breeds the tissue culture method of seedling |
| CN109169275A (en) * | 2018-09-13 | 2019-01-11 | 甘肃源宜生物科技有限公司 | A kind of pale reddish brown trident bletilla striata tissue-culturing rapid propagation culture medium and method |
| CN109197591A (en) * | 2018-10-24 | 2019-01-15 | 河南云帮农业科技有限公司 | A kind of excellent bletilla striata tissue culture method |
| CN109452171A (en) * | 2018-11-26 | 2019-03-12 | 丽江海贝瑞生物科技有限公司 | A kind of tissue culture method of the sterile induction plant regeneration of pale reddish brown trident bletilla striata seeds |
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| CN103168685A (en) * | 2013-03-15 | 2013-06-26 | 浙江中医药大学 | Method for cultivating rhizoma bletillae polyploidy plants |
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Cited By (23)
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| CN104145816B (en) * | 2014-07-29 | 2016-03-23 | 郎溪县紫兰药材种植专业合作社 | Bletilla striata tissue culture method |
| CN104145816A (en) * | 2014-07-29 | 2014-11-19 | 郎溪县紫兰药材种植专业合作社 | Bletilla striata tissue-culture seedling raising method |
| CN104335903A (en) * | 2014-11-21 | 2015-02-11 | 广西中医药大学 | Method for accelerating quick propagation of rhizoma bletillae |
| CN104686346A (en) * | 2015-02-24 | 2015-06-10 | 陈桂容 | Tissue culture and rapid propagation method for divaricate saposhnikovia root |
| CN104770293A (en) * | 2015-04-01 | 2015-07-15 | 张子国 | Bletilla open-type tissue culture seedling method |
| CN104920208A (en) * | 2015-05-15 | 2015-09-23 | 浙江虹越花卉股份有限公司 | Culture medium for rapid propagation in tissue culture of bletilla striata |
| CN104904597A (en) * | 2015-05-28 | 2015-09-16 | 云南省农业科学院农业环境资源研究所 | Bletilla striata rapid propagation and sunshine seedling strengthening method |
| CN104871977A (en) * | 2015-05-29 | 2015-09-02 | 江苏农林职业技术学院 | Bletilla striata tissue culture medium and its preparing method |
| CN105145352B (en) * | 2015-08-03 | 2017-06-06 | 河南科技大学 | A kind of bletilla seed height of seedling imitates group culturation rapid propagating technology |
| CN105145352A (en) * | 2015-08-03 | 2015-12-16 | 河南科技大学 | Efficient tissue culture and rapid propagation technology for seedlings of bletilla striata |
| CN105210875A (en) * | 2015-10-15 | 2016-01-06 | 铜仁市金农绿色农业科技有限公司 | A kind of bletilla striata quick propagation method |
| CN105766645A (en) * | 2016-03-31 | 2016-07-20 | 广西医科大学 | Efficient reproduction method of rhizoma bletillae tissue culture seedlings |
| CN105766645B (en) * | 2016-03-31 | 2018-07-24 | 广西医科大学 | Bletilla tissue-cultured seedling efficient propagation method |
| CN106665349A (en) * | 2016-11-29 | 2017-05-17 | 重庆上古药业有限公司 | Planting method of bletilla |
| CN106665349B (en) * | 2016-11-29 | 2018-06-19 | 重庆上古药业有限公司 | A kind of implantation methods of the bletilla striata |
| CN107173222A (en) * | 2017-03-27 | 2017-09-19 | 河池乐康生态农业科技有限公司 | A kind of wild bletilla striata quick breeding method for tissue culture |
| CN107047310A (en) * | 2017-05-10 | 2017-08-18 | 文山学院 | A kind of cultural method of bletilla striata seeds culture seedling |
| CN107466654A (en) * | 2017-09-12 | 2017-12-15 | 六盘水黔丰农业发展有限公司 | A kind of efficiently method of reproducing bletilla striata tissue-cultured seedling and the growth belt method of the bletilla striata |
| CN108055993A (en) * | 2017-12-01 | 2018-05-22 | 亳州学院 | The nutrient solution method that Dabie Mountain wild bletilla striata seedling stage sprouts |
| CN108094209A (en) * | 2017-12-28 | 2018-06-01 | 临沧市云瑞堂生物科技有限公司 | A kind of bletilla striata quickly breeds the tissue culture method of seedling |
| CN109169275A (en) * | 2018-09-13 | 2019-01-11 | 甘肃源宜生物科技有限公司 | A kind of pale reddish brown trident bletilla striata tissue-culturing rapid propagation culture medium and method |
| CN109197591A (en) * | 2018-10-24 | 2019-01-15 | 河南云帮农业科技有限公司 | A kind of excellent bletilla striata tissue culture method |
| CN109452171A (en) * | 2018-11-26 | 2019-03-12 | 丽江海贝瑞生物科技有限公司 | A kind of tissue culture method of the sterile induction plant regeneration of pale reddish brown trident bletilla striata seeds |
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