CN103642701B - Application of microbial preparation for quickly decomposing cattle faeces - Google Patents
Application of microbial preparation for quickly decomposing cattle faeces Download PDFInfo
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- CN103642701B CN103642701B CN201310653376.8A CN201310653376A CN103642701B CN 103642701 B CN103642701 B CN 103642701B CN 201310653376 A CN201310653376 A CN 201310653376A CN 103642701 B CN103642701 B CN 103642701B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a microbial preparation of quickly decomposing cattle faeces and an application of the microbial preparation, and relates to a microbial preparation capable of decomposing cattle faeces as well as a preparation method and application of the microbial preparation. The invention aims to solve the problem that in the existing decomposing and fermenting process of cattle faeces, the manure is quick to warm, the degradative rate for cellulose is low, the decomposing degree is not high and the fermenting period is long. The microbial preparation is prepared from long thorned chaetomium, trichoderma koningii, chaetomium torulosum Bainier, Trichoderma viride and cryytococcus neoformans. The preparation method comprises the following steps: I, inoculating a strain on a culture medium; II, preparing a skimmed milk emulsion, inoculating after sterilization and producing a leavening agent on a table; III, carrying out quick freezing on the leavening agent and freezing and drying to obtain the freeze-drying culture powder; and IV, proportioning the freeze-drying culture powder according to weight part to prepare the microbial preparation after uniformly mixing. By fermenting cattle faeces with the microbial preparation, the microbial preparation is quick to warm, and can improve the degree of cellulose degradation and the degree of decomposition of organic fertilizer products and shorten the fermenting period.
Description
Technical field
The present invention relates to microbial preparation of a kind of Feces of Beef Cattle that can become thoroughly decomposed and its preparation method and application.
Background technology
China's steer ration industry is fast-developing in recent years, but produces a large amount of ight soil and sewage thereupon, not only has influence on the Sustainable development of related industries, also causes serious rural environmental pollution and harm humans is healthy.Night soil-treatment measure both at home and abroad at present is all tended to produce fertilizer, and its technique mainly contains convection drying, Natural compost and microbial inoculant fermentation etc.Cow dung contains organic 14.5%, nitrogen (N) 0.30-0.45%, phosphorus (P
2o
5) 0.15-0.25%, potassium (K
2o) 0.10-0.15%, compares organic in cow dung with other domestic animals and nutrient content is minimum in various domestic animal, and be of close texture, fibre content is high simultaneously, moisture more, and decompose slow, thermal value is low, belongs to slow acting fertilizer.Thus the method making tradition utilize Feces of Beef Cattle Natural compost to produce fertilizer deposits intensification during the fermentation, and slowly, cellulose degradation rate is low, and degree of becoming thoroughly decomposed is not high, the problem that fermentation period is long.
Microbial inoculant fermentation is by adding functional microorganism on the basis of compost, beneficial microorganism is utilized to breed rapidly replacement indigenous microorganism, regulate compost Bacterial community, improve beneficial microorganism activity, by mutually acting synergistically between each bacterial classification, form the complicated and stable ecosystem, the organic materials organic matters such as fast decoupled ight soil and stalk produce heat energy, organic materials material temperature degree is raised rapidly, suppress or kill the harmful organism such as germ, worm's ovum, improve degree of becoming thoroughly decomposed, produce biological organic fertilizer truly.The choose reasonable of microbial strains combination is the key affecting organic fertilizer production effect, utilize the bacterial classification of fermentable animal waste production fertilizer a lot of at present both at home and abroad, but mainly for the non-ruminant animal such as pig, fowl night soil-treatment successful, the Feces of Beef Cattle treatment effect high to fibre content is unsatisfactory.
Summary of the invention
The object of the invention is to become thoroughly decomposed the intensification of fermenting process muck slowly to solve existing Feces of Beef Cattle, cellulose degradation rate is low, the problem that degree of becoming thoroughly decomposed is not high and fermentation period is long, and microbial preparation of a kind of Feces of Beef Cattle quick composting and its preparation method and application is provided.
The microbial preparation of Feces of Beef Cattle quick composting of the present invention is that, the round ferment hair shell of 3 ~ 5 part, the viride of 3 ~ 5 part and the geotrichum candidum of 2 ~ 3 part mould by the lunge hair shell of 5 ~ 7 parts, the healthy and free from worry wood of 4 ~ 6 parts by each freeze-dried vaccine powder weight are made.
The preparation method of the microbial preparation of Feces of Beef Cattle quick composting of the present invention follows these steps to realize:
One, PDA substratum and malt juice liquid medium are carried out sterilizing 20 minutes at the temperature of 121 DEG C, then 30 DEG C are cooled to, again lunge hair shell, healthy and free from worry wood mould, round ferment hair shell, viride 4 strain bacterial strain are inoculated in 4 parts of PDA substratum respectively, 1 strain geotrichum candidum bacterial strain is inoculated in malt juice liquid medium, cultivate 24 hours at 28 DEG C of shaking tables, obtain 5 strain mother starters;
Two, the skimmed milk emulsion of 10% is prepared, 2% liquid glycerin, 1% xylo-oligosaccharide, 1% glucose is added in skimmed milk emulsion, 121 DEG C of sterilizings are cooled to 30 DEG C in 20 minutes, 5 strain mother starters are inoculated respectively by 2% volume ratio, 28 DEG C of shaking tables cultivate 48 hours, obtain 5 strain productive leavening agent;
Three, 5 strain productive leavening agent step 2 obtained respectively aseptically import in 5 glass ampoules, and add a cover the laggard row fast frozen of bottle stopper, the starter after freezing is transferred to Freeze Drying Equipment and carried out lyophilize, obtain 5 strain freeze-drying lactobacillus powder;
Four, take 5 ~ 7 parts, lunge hair shell freeze-drying lactobacillus powder, 4 ~ 6 parts, the mould freeze-drying lactobacillus powder of healthy and free from worry wood, 3 ~ 5 parts, circle ferment hair shell freeze-drying lactobacillus powder, 3 ~ 5 parts, viride freeze-drying lactobacillus powder and 2 ~ 3 parts, geotrichum candidum freeze-drying lactobacillus powder by weight, fully load the microbial preparation that vacuum bag obtains Feces of Beef Cattle quick composting after mixing.
The method applying the microbial preparation fermentation Feces of Beef Cattle of Feces of Beef Cattle quick composting of the present invention follows these steps to realize:
One, fresh Feces of Beef Cattle, stalk, the peat composed of rotten mosses, beet pulp, residue from beans after making and zeolite powder are mixed, stir and obtain organic materials;
Two, 30 ~ 35 DEG C of warm water that the microbial preparation of Feces of Beef Cattle quick composting adds the ratio 1000g of 50g in process organic materials per ton are dissolved, 10 ~ 20g brown sugar is added in warm water, evenly spraying with atomizer or watering can after placing 10 ~ 14 hours at normal temperatures adds in organic materials, heap fermentation at normal temperatures, every turning in 3 days once, fermentation obtains the Feces of Beef Cattle fertilizer of fermenting-ripening for 12 days.
The present invention is directed to that Feces of Beef Cattle is organic and nutrient content is minimum in various animal waste, be of close texture simultaneously, fibre content is high, moisture more, decompose slow, the feature that thermal value is low, carry out quick fermentation Feces of Beef Cattle produce fertilizer by screening best strain combination, the microbial preparation of this Feces of Beef Cattle quick composting can be reduced to 70.3% by cellulosic degradation rate in fermentation the 12nd angel's compost, sustainable 8 days of high-temperature time, improve the rotten degree of cellulose degradation and organic fertilizer product, shorten fermentation period, thus improve fermentation efficiency and the organic fertilizer quality of beef cattle excrement.
Embodiment
Embodiment one: the microbial preparation of present embodiment Feces of Beef Cattle quick composting is that, the round ferment hair shell of 3 ~ 5 part, the viride of 3 ~ 5 part and the geotrichum candidum of 2 ~ 3 part mould by the lunge hair shell of 5 ~ 7 parts, the healthy and free from worry wood of 4 ~ 6 parts by each freeze-dried vaccine powder weight are made.
Lunge hair shell described in present embodiment, healthy and free from worry wood mould, round ferment hair shell, viride and this 5 strain bacterium of geotrichum candidum are all purchased from China General Microbiological culture presevation administrative center (CGMCC), and wherein the deposit number of lunge hair shell (Chaetomiumdolichotrichum) is CGMCC No:3.3616; The deposit number of healthy and free from worry wood mould (Trichoderma koningii) is CGMCC No:3.4033; The deposit number of circle ferment hair shell (Chaetomium torulosum) is CGMCCNo:3.3617; The deposit number of viride (Trichoderma viride) is CGMCC No:3.3744; The deposit number of geotrichum candidum (Geotrichum candidum) is CGMCC No:2.1062.
Fertilizer is made with the microbial preparation of the Feces of Beef Cattle quick composting of present embodiment, significantly can accelerate Feces of Beef Cattle to become thoroughly decomposed the heat-up rate of fermenting process, extend high-temperature time, improve the rotten degree of cellulose degradation and organic fertilizer product, shorten fermentation period, and then improve cow excrement fermentation efficiency and organic fertilizer quality.
Embodiment two: the microbial preparation of present embodiment Feces of Beef Cattle quick composting is that, the round ferment hair shell of 4 part, the viride of 4 part and the geotrichum candidum of 2 part mould by the lunge hair shell of 6 parts, the healthy and free from worry wood of 5 parts by each freeze-dried vaccine powder weight are made.
Embodiment three: the preparation method of the microbial preparation of present embodiment Feces of Beef Cattle quick composting follows these steps to implement:
One, PDA substratum and malt juice liquid medium are carried out sterilizing 20 minutes at the temperature of 121 DEG C, then 30 DEG C are cooled to, again lunge hair shell, healthy and free from worry wood mould, round ferment hair shell, viride 4 strain bacterial strain are inoculated in 4 parts of PDA substratum respectively, 1 strain geotrichum candidum bacterial strain is inoculated in malt juice liquid medium, cultivate 24 hours at 28 DEG C of shaking tables, obtain 5 strain mother starters;
Two, the skimmed milk emulsion of 10% is prepared, 2% liquid glycerin, 1% xylo-oligosaccharide, 1% glucose is added in skimmed milk emulsion, 121 DEG C of sterilizings are cooled to 30 DEG C in 20 minutes, 5 strain mother starters are inoculated respectively by 2% volume ratio, 28 DEG C of shaking tables cultivate 48 hours, obtain 5 strain productive leavening agent;
Three, 5 strain productive leavening agent step 2 obtained respectively aseptically import in 5 glass ampoules, and add a cover the laggard row fast frozen of bottle stopper, the starter after freezing is transferred to Freeze Drying Equipment and carried out lyophilize, obtain 5 strain freeze-drying lactobacillus powder;
Four, take 5 ~ 7 parts, lunge hair shell freeze-drying lactobacillus powder, 4 ~ 6 parts, the mould freeze-drying lactobacillus powder of healthy and free from worry wood, 3 ~ 5 parts, circle ferment hair shell freeze-drying lactobacillus powder, 3 ~ 5 parts, viride freeze-drying lactobacillus powder and 2 ~ 3 parts, geotrichum candidum freeze-drying lactobacillus powder by weight, fully load the microbial preparation that vacuum bag obtains Feces of Beef Cattle quick composting after mixing.
Each Material Percentage involved by present embodiment step 2 preparation skimmed milk emulsion all refers to mass percent, and the fast frozen described in step 3 processes in-30 DEG C of refrigerators.
Embodiment four: present embodiment and embodiment three are all undertaken inoculating by 10% of culture volume unlike the lunge hair shell described in step one, healthy and free from worry wood mould, round ferment hair shell, viride and geotrichum candidum.Other step and parameter identical with embodiment three.
Embodiment five: the method for the microbial preparation fermentation Feces of Beef Cattle of the Feces of Beef Cattle quick composting of application implementation mode one follows these steps to implement:
One, fresh Feces of Beef Cattle, stalk, the peat composed of rotten mosses, beet pulp, residue from beans after making and zeolite powder are mixed, stir and obtain organic materials;
Two, 30 ~ 35 DEG C of warm water that the microbial preparation of Feces of Beef Cattle quick composting adds the ratio 1000g of 50g in process organic materials per ton are dissolved, 10 ~ 20g brown sugar is added in warm water, evenly spraying with atomizer or watering can after placing 10 ~ 14 hours at normal temperatures adds in organic materials, heap fermentation at normal temperatures, every turning in 3 days once, fermentation obtains the Feces of Beef Cattle fertilizer of fermenting-ripening for 12 days.
Embodiment six: present embodiment and embodiment five unlike step one by weight percentage by the fresh Feces of Beef Cattle of 70%, 13% stalk, 9% the peat composed of rotten mosses, the beet pulp of 3.5%, the residue from beans after making of 3% and 1.5% zeolite powder mix.Other step and parameter identical with embodiment five.
Embodiment seven: present embodiment and embodiment five or six are corn stalk, beanstalk, paddy rice straw or wheat straw unlike the stalk described in step one.Other step and parameter identical with embodiment five or six.
Embodiment one: the preparation method of the microbial preparation of the present embodiment Feces of Beef Cattle quick composting follows these steps to realize:
One, PDA substratum and malt juice liquid medium are carried out sterilizing 20 minutes at the temperature of 121 DEG C, then 30 DEG C are cooled to, again lunge hair shell, healthy and free from worry wood mould, round ferment hair shell, viride 4 strain bacterial strain are inoculated in 4 parts of PDA substratum respectively respectively by 10% of culture volume, 1 strain geotrichum candidum bacterial strain is inoculated in malt juice liquid medium, cultivate 24 hours at 28 DEG C of shaking tables, obtain 5 strain mother starters;
Two, the skimmed milk emulsion of 10% is prepared, 2% liquid glycerin, 1% xylo-oligosaccharide, 1% glucose is added in skimmed milk emulsion, 121 DEG C of sterilizings are cooled to 30 DEG C in 20 minutes, 5 strain mother starters are inoculated respectively by 2% volume ratio, 28 DEG C of shaking tables cultivate 48 hours, obtain 5 strain productive leavening agent;
Three, 5 strain productive leavening agent step 2 obtained respectively aseptically import in 5 glass ampoules, and add a cover the laggard row fast frozen of bottle stopper, the starter after freezing is transferred to Freeze Drying Equipment and carried out lyophilize, obtain 5 strain freeze-drying lactobacillus powder;
Four, take 6 parts, lunge hair shell freeze-drying lactobacillus powder, 5 parts, the mould freeze-drying lactobacillus powder of healthy and free from worry wood, 4 parts, circle ferment hair shell freeze-drying lactobacillus powder, 4 parts, viride freeze-drying lactobacillus powder and 2 parts, geotrichum candidum freeze-drying lactobacillus powder by weight, fully load the microbial preparation that vacuum bag obtains Feces of Beef Cattle quick composting after mixing.
PDA substratum described in the present embodiment step one and malt juice liquid medium press the method preparation that Microbiology Experiment handbook describes.
The method of the microbial preparation fermentation Feces of Beef Cattle of the Feces of Beef Cattle quick composting of Application Example follows these steps to implement:
One, by weight percentage by the fresh Feces of Beef Cattle of 70%, 13% stalk, 9% the peat composed of rotten mosses, the beet pulp of 3.5%, the residue from beans after making of 3% and 1.5% zeolite powder mixing, stir and obtain organic materials;
Two, the ratio of 50g is added first by the 1000g warm water dissolving of the microbial preparation of Feces of Beef Cattle quick composting with 30 DEG C in organic materials per ton, and then add 15g brown sugar, placement at normal temperatures is evenly sprayed with atomizer after 12 hours and is added in organic materials, heap fermentation at normal temperatures, every turning in 3 days once, fermentation obtains the Feces of Beef Cattle fertilizer of fermenting-ripening for 12 days.
By carrying out Piglet s colibacillosis in beef cattle field, arrange test group and control group respectively, test group is by method process organic materials described in embodiment, and control group is the fermentation of conventional Natural compost.The data obtained is comprehensively analyzed in addition to the results are as follows:
1, test group arrives hot stage (>50 DEG C) on the 4th day in fermentation, and comparatively control group shifts to an earlier date 3 days; Test group the 7th day heap temperature reaches maximum, average out to 71.2 DEG C, and comparatively control group shifts to an earlier date 5 days and is significantly higher than the highest leavening temperature 63.5 DEG C; Test group high temperature continues 8 days, and comparatively control group extends 2 days.Result shows that present method can improve the leavening temperature of organic materials fast, and the thermophilic fermentation time remains long, makes organic materials fully slaking fast, and then accelerates compost fermentation speed, shorten the fermented manure time.
2, cellulose degradation rate aspect, test group cellulose degradation rate in fermentation the 12nd day compost is 70.3%, and control group is 43.5%, and test group comparatively control group improves 61.6%, and difference all reaches conspicuous level (P < 0.05).This test-results shows, present method process can significantly improve cellulosic degraded, and then promotes organic materials fully slaking fast.
3, test group is 85.3% in the germination index of fermentation the 12nd day compost, and when control group compost proceeds to the 28th day, germination index is 85.1%, and test group comparatively control group shifts to an earlier date 16 days.Seed germination index (GI) is the important parameter judging compost toxicity and rotten degree, thinks when germination index reaches 85% in theory, can think compost do not had phytotoxicity in other words compost become thoroughly decomposed.This test-results shows, present method process organic materials can accelerate the rotten degree of compost, shortens the compost time.
Claims (2)
1. an application for the microbial preparation of Feces of Beef Cattle quick composting, is characterized in that the method for the microbial preparation fermentation Feces of Beef Cattle applying Feces of Beef Cattle quick composting follows these steps to realize:
One, by weight percentage by the fresh Feces of Beef Cattle of 70%, 13% stalk, 9% the peat composed of rotten mosses, the beet pulp of 3.5%, the residue from beans after making of 3% and 1.5% zeolite powder mixing, stir and obtain organic materials;
Two, 30 ~ 35 DEG C of warm water that the microbial preparation of Feces of Beef Cattle quick composting adds the ratio 1000g of 50g in process organic materials per ton are dissolved, 10 ~ 20g brown sugar is added in warm water, evenly spraying with atomizer or watering can after placing 10 ~ 14 hours at normal temperatures adds in organic materials, heap fermentation at normal temperatures, every turning in 3 days once, fermentation obtains the Feces of Beef Cattle fertilizer of fermenting-ripening for 12 days;
Wherein the microbial preparation of Feces of Beef Cattle quick composting is that, the round ferment hair shell of 3 ~ 5 part, the viride of 3 ~ 5 part and the geotrichum candidum of 2 ~ 3 part mould by the lunge hair shell of 5 ~ 7 parts, the healthy and free from worry wood of 4 ~ 6 parts by each freeze-dried vaccine powder weight are made.
2. the application of the microbial preparation of a kind of Feces of Beef Cattle quick composting according to claim 1, is characterized in that the stalk described in step one is corn stalk, beanstalk, paddy rice straw or wheat straw.
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| CN107586733A (en) * | 2016-07-08 | 2018-01-16 | 潍坊盛泉生物科技有限公司 | It is a kind of to be used to handle microorganism formulation of feces of livestock and poultry and preparation method thereof |
| CN106673916A (en) * | 2017-02-03 | 2017-05-17 | 北京皕富园生态农业有限公司 | Compound starter and preparation method and application thereof |
| CN114015581A (en) * | 2021-11-05 | 2022-02-08 | 中国科学院东北地理与农业生态研究所 | Preparation method of beef cattle manure rapid-decomposition leavening agent |
| CN114455987A (en) * | 2022-01-25 | 2022-05-10 | 广西大学 | Beef cattle manure and urine integrated bio-organic fertilizer and preparation method thereof |
Citations (1)
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| CN1288876A (en) * | 2000-11-14 | 2001-03-28 | 张健伦 | Organic compound fertilizer producing method |
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| CN1288876A (en) * | 2000-11-14 | 2001-03-28 | 张健伦 | Organic compound fertilizer producing method |
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| Title |
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| 嗜热真菌的生物转化功能与经济价值;连宾，等;《地球与环境》;20041231;第32 卷(第2 期);49-54 * |
| 袁立,等.国内外牛粪生物质资源利用的现状与趋势imag.《中国奶牛》.2011,3-9. * |
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