CN103601844A - Novel animal specimen preservation gel as well as preparation method and use thereof - Google Patents
Novel animal specimen preservation gel as well as preparation method and use thereof Download PDFInfo
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- CN103601844A CN103601844A CN201310567766.3A CN201310567766A CN103601844A CN 103601844 A CN103601844 A CN 103601844A CN 201310567766 A CN201310567766 A CN 201310567766A CN 103601844 A CN103601844 A CN 103601844A
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- gel
- novel
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- preservation
- preserved
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- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 241001465754 Metazoa Species 0.000 title abstract description 12
- 238000004321 preservation Methods 0.000 title abstract description 12
- 238000001879 gelation Methods 0.000 title 1
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims abstract description 7
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims abstract description 7
- 239000002994 raw material Substances 0.000 claims abstract description 7
- 235000019333 sodium laurylsulphate Nutrition 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims description 7
- 239000004160 Ammonium persulphate Substances 0.000 claims description 6
- 239000004141 Sodium laurylsulphate Substances 0.000 claims description 6
- 235000019395 ammonium persulphate Nutrition 0.000 claims description 6
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 6
- 241000196324 Embryophyta Species 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 4
- 230000001954 sterilising effect Effects 0.000 abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 4
- -1 acrylamide compound Chemical class 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 230000006378 damage Effects 0.000 abstract 2
- 239000011837 N,N-methylenebisacrylamide Substances 0.000 abstract 1
- 208000027418 Wounds and injury Diseases 0.000 abstract 1
- 229910001870 ammonium persulfate Inorganic materials 0.000 abstract 1
- 238000009395 breeding Methods 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 abstract 1
- 238000005536 corrosion prevention Methods 0.000 abstract 1
- 208000014674 injury Diseases 0.000 abstract 1
- 231100000053 low toxicity Toxicity 0.000 abstract 1
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 17
- 239000000243 solution Substances 0.000 description 11
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- 241000252229 Carassius auratus Species 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 230000008859 change Effects 0.000 description 5
- 239000002932 luster Substances 0.000 description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 150000001298 alcohols Chemical class 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 239000008098 formaldehyde solution Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229920000592 inorganic polymer Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000003206 sterilizing agent Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
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Abstract
The invention relates to a novel animal specimen preservation gel as well as a preparation method and use thereof and belongs to the field of environment-friendly novel materials. The gel is prepared from the raw materials in percentage by mass: 1-10% of acrylamide compound, 15-30% of Tris-HCl, 0.1-0.5% of N,N-methylene-bis-acrylamide, 5-20% of sodium lauryl sulfate, 0.5-1.0% of ammonium persulfate and 38.5-78.5% of solution which is prepared from water and has the pH of 3.5-5.0. The pH is 3.5-5.0, so that animals and plants are effectively inhibited from breeding bacteria and molds; the animal specimen preservation gel disclosed by the invention has good effects on preservation, fixation, sterilization and corrosion prevention; the preservation gel is simple and convenient to prepare, the raw materials are all low-toxicity environment-friendly chemicals, and the injury/harm to the human/environment is little, so that the gel is a very good product for producing an animal/plant specimen and is convenient to transport.
Description
Technical field
The present invention relates to a kind of novel zoological specimens and preserve gel, belong to environmental friendly material field.
Technical background
As everyone knows, sample is kept in transparent liquid, sample is kept intact as far as possible, be conducive to viewing and admiring of scientific research, teaching and people, current used liquid is mainly formaldehyde solution and has the allotment of volatile alcohol solution and form, but formaldehyde has strong impulse smell, in making and use procedure, not only contaminate environment, the more important thing is and be detrimental to health, also comparatively inconvenience while transporting in addition.
Ethanol medically can be used as sterilizing agent and sterilant, be subject to the inspiration of this traditional method, develop in recent years and using the preservation liquid for animal body specimen of ethanol as effective constituent, as Chinese patent CN88105221A discloses a kind of biological sample, protect look sanitas, it is that the materials such as ethanol, potassium acetate, sodium sulfate, glycerol, water are mixed with to mixed solution in proportion.But yet this preservation liquid life-time service animal proteinum hardening distortion.
Summary of the invention
The object of the present invention is to provide a kind of zoological specimens that do not contain formaldehyde or alcohols to preserve gel.Another object of the present invention is to provide a kind of method of preserving zoological specimens better effects if.
The inventor is surprised to find that: by selecting suitable component proportion can make a kind of transparent gel, animal is sealed up for safekeeping, not only overcome the above-mentioned shortcoming causing of formaldehyde and alcohols specimen preserving liquid, and there is better preservation effect, and this composition final state is solid gel, well preserved moisture and the state of sample, made again transportation become simple and convenient.
The preparation method that a kind of novel zoological specimens provided by the present invention are preserved gel, its desired raw material component and quality proportioning are as follows:
The preparation method that described novel zoological specimens are preserved gel is:
By acrylamide, Tris-HCl, N, the PH that N-methylene-bisacrylamide, sodium lauryl sulphate and water form is that 3.5-5.0 solution prepares and puts in previously prepd sample bottle, add ammonium persulphate to wait for 3-5 minutes, solution solidifies becomes gel.Obtain novel zoological specimens and preserve gel.
The simultaneously claimed novel zoological specimens that utilize present method and make of the present invention are preserved gel.
In actual use, by acrylamide, Tris-HCl, N, the PH that N-methylene-bisacrylamide, sodium lauryl sulphate and water form is that 3.5-5.0 solution prepares and puts in previously prepd sample bottle, by animal to be preserved or careful the putting into wherein of carcase, add ammonium persulphate to wait for 3-5 minutes, solution solidifies becomes gel again.
Preferably, carcase is cooked sterilization pretreatment, is expected to obtain better effect.
The gel that adopts above-mentioned technique means and form is to the figurate effect of animal tool, and compared to liquid preservative solution, gel is as clear as crystal, has no irritating odor, and transports also very convenient.The solution producing in process for preparation be PH be 3.5-5.0 acidic solution, there is sterilization, rot-resistant effect.Facts have proved, preserve after 2 years, the state of zoological specimens (color and luster, shape etc.) does not all change.
The present invention need not add any sanitas, because this gel self is just isolated by materials such as oxygen, and the gel ultimate constituent is polymer inorganic polymer, and PH 3.5-5.0 has effectively suppressed growing of animals and plants bacterium and mould.
Zoological specimens of the present invention preserve gel and have that preparation is simple, raw material is easy to get, to advantages such as human body and environment have no side effect, can be widely used in preparation of specimen's work in the scientific popularization places such as museum, school.Also the urgent preservation that can be applicable to zoologist's field acquisition sample, has broad application prospects and social value.
Accompanying drawing explanation
The present invention has accompanying drawing 4 width, for obtained the photo of sample according to embodiment 1.Wherein:
Fig. 1 is upright front view I;
Fig. 2 is upright front view II;
Fig. 3 is upright side-view I;
Fig. 4 is upright side-view II.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, but these embodiment do not limit the scope of the invention.
Embodiment 1
The acrylamide compound that is 1%% by mass ratio, Tris-HCl of 30%, 0.1% N, the solution that the PH that N-methylene-bisacrylamide, 20% sodium lauryl sulphate and 38.5% water form is 5.0 prepares to be put in previously prepd sample bottle, small goldfish corpse is put into and wherein added 0.5% ammonium persulphate to wait for 3 minutes, and solution solidifies becomes gel.Obtain small goldfish gel sample.
Sample was observed in 1 month, 3 months, 6 months, 12 months, 24 months after sample preparation, finds that state, color and luster, the shape of small goldfish all changes without obvious respectively.
Latter 24th month of sample preparation, small goldfish taking-up is rinsed to the rear observation of taking out, the state of discovery small goldfish, color and luster, shape, all without any change, are placed in normal indoor environment afterwards, within 3 days, observe afterwards discovery small goldfish and have just started to decay rotten.
Embodiment 2
The acrylamide compound that is 10% by mass ratio, Tris-HCl of 15%, 0.5% N, the solution that the PH that N-methylene-bisacrylamide, 5% sodium lauryl sulphate and 78.5% water form is 3.5 prepares to be put in previously prepd sample bottle, little conch corpse is put into and wherein added 1.0% ammonium persulphate to wait for 3-5 minutes, solution solidifies becomes gel after 2 years, and little conch state, pool become, shape does not all change.
Little conch sample was observed in 1 month, 3 months, 6 months, 12 months, 24 months after sample preparation respectively, finds that the state, color and luster, shape of little conch are all without any change.After sample preparation 24th month, little conch is taken out after rinsing and takes out and observe, find that the state, color and luster, shape of little conch, all without any change, are placed in normal indoor environment afterwards, 3 days afterwards the little conch of observation discovery start to decay rotten.
Zoological specimens of the present invention are preserved gel and are had good preservation, fixing, sterilization, preservative activity.And inferior preservation gel making is simple and convenient and raw material is low toxic and environment-friendly chemical, very little to human body and environmental hazard, be to make the good product of animal or plant specimen and be convenient to transportation.Can be widely used in preparation of specimen's work in the scientific popularization places such as museum, school.Also the urgent preservation that can be applicable to zoologist's field acquisition sample, has broad application prospects and social value.
Claims (3)
1. novel zoological specimens are preserved a preparation method for gel, and its desired raw material component and quality proportioning are as follows:
Its preparation process is:
By acrylamide, Tris-HCl, N, the PH that N-methylene-bisacrylamide, sodium lauryl sulphate and water form is that 3.5-5.0 solution prepares and puts in previously prepd sample bottle, add ammonium persulphate to wait for 3-5 minutes, solution solidifies becomes gel, obtains novel zoological specimens and preserves gel.
2. novel zoological specimens are preserved a gel, it is characterized in that: be to prepare in accordance with the method for claim 1 and obtain.
3. novel zoological specimens are preserved a purposes for gel, are the making for zoological specimens, and its desired raw material component and quality proportioning are as follows:
By acrylamide, Tris-HCl, N, the PH that N-methylene-bisacrylamide, sodium lauryl sulphate and water form is that 3.5-5.0 solution prepares and puts in previously prepd sample bottle, by careful the putting into wherein of carcase to be preserved, add ammonium persulphate to wait for 3-5 minutes, solution solidifies becomes gel again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310567766.3A CN103601844B (en) | 2013-11-14 | 2013-11-14 | A kind of zoological specimens preserve gel and its production and use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310567766.3A CN103601844B (en) | 2013-11-14 | 2013-11-14 | A kind of zoological specimens preserve gel and its production and use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103601844A true CN103601844A (en) | 2014-02-26 |
| CN103601844B CN103601844B (en) | 2016-02-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310567766.3A Expired - Fee Related CN103601844B (en) | 2013-11-14 | 2013-11-14 | A kind of zoological specimens preserve gel and its production and use |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103601844B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042622A (en) * | 2018-09-28 | 2018-12-21 | 河南科技大学 | A kind of guarantor's color fixing agent and preparation method thereof and the application in prepared by sample |
| CN109166439A (en) * | 2018-10-30 | 2019-01-08 | 河南科技大学 | A kind of production method of the stomach section silica gel model of sheep |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3428470A (en) * | 1966-06-09 | 1969-02-18 | Dow Chemical Co | Biological specimen in a cross-linked water-swellable acrylamide type resin and the incorporation of the specimen therein |
| US3638709A (en) * | 1969-12-11 | 1972-02-01 | Dow Chemical Co | Method of suspending immobilized biological specimens in a transparent gel in a transparent container |
| CN88105221A (en) * | 1988-03-08 | 1988-10-19 | 王向前 | Preparation method of color-preserving preservative for biological specimens |
| CN101718745A (en) * | 2009-12-07 | 2010-06-02 | 南京农业大学 | Dimensional electrophoresis method for total protein of jute root system |
-
2013
- 2013-11-14 CN CN201310567766.3A patent/CN103601844B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3428470A (en) * | 1966-06-09 | 1969-02-18 | Dow Chemical Co | Biological specimen in a cross-linked water-swellable acrylamide type resin and the incorporation of the specimen therein |
| US3638709A (en) * | 1969-12-11 | 1972-02-01 | Dow Chemical Co | Method of suspending immobilized biological specimens in a transparent gel in a transparent container |
| CN88105221A (en) * | 1988-03-08 | 1988-10-19 | 王向前 | Preparation method of color-preserving preservative for biological specimens |
| CN101718745A (en) * | 2009-12-07 | 2010-06-02 | 南京农业大学 | Dimensional electrophoresis method for total protein of jute root system |
Non-Patent Citations (3)
| Title |
|---|
| 刘仰斌等: ""改良人体标本保存液配方的效果"", 《中国组织工程研究与临床康复》 * |
| 李成文: "《现代免疫化学技术》", 30 April 1992, 上海科学技术出版社 * |
| 王廷华等: "《抗体理论技术》", 31 March 2005, 北京：科学出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042622A (en) * | 2018-09-28 | 2018-12-21 | 河南科技大学 | A kind of guarantor's color fixing agent and preparation method thereof and the application in prepared by sample |
| CN109166439A (en) * | 2018-10-30 | 2019-01-08 | 河南科技大学 | A kind of production method of the stomach section silica gel model of sheep |
| CN109166439B (en) * | 2018-10-30 | 2020-11-13 | 河南科技大学 | Method for manufacturing sheep gastric section silica gel model |
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| Publication number | Publication date |
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| CN103601844B (en) | 2016-02-10 |
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| PB01 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Chunbin Inventor after: Ma Hongfeng Inventor after: Zhang Zhongjun Inventor before: Ma Hongfeng Inventor before: Li Chunbin Inventor before: Zhang Zhongjun |
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| COR | Change of bibliographic data | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20160113 Address after: 116600 Dalian economic and Technological Development Zone, Liaohe Road, No. 18, Liaoning Applicant after: Dalian ethnic university Applicant after: Dalian Jinguiyuan Technology Development Co., Ltd. Address before: 116600 Harbin Road, Liaoning city of Dalian Province Economic and Technological Development Zone No. 34 Dalian Jingui edge technology development Co. Ltd. Applicant before: Dalian Jinguiyuan Technology Development Co., Ltd. |
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| C14 | Grant of patent or utility model | ||
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Granted publication date: 20160210 Termination date: 20171114 |