CN103549386B - A kind of compound Chinese caterpillar fungus-lucid ganoderma composition and preparation method thereof - Google Patents
A kind of compound Chinese caterpillar fungus-lucid ganoderma composition and preparation method thereof Download PDFInfo
- Publication number
- CN103549386B CN103549386B CN201310507737.8A CN201310507737A CN103549386B CN 103549386 B CN103549386 B CN 103549386B CN 201310507737 A CN201310507737 A CN 201310507737A CN 103549386 B CN103549386 B CN 103549386B
- Authority
- CN
- China
- Prior art keywords
- powder
- percent
- portions
- ganoderma lucidum
- follows
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 25
- 241000222336 Ganoderma Species 0.000 title claims abstract description 22
- 150000001875 compounds Chemical class 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 240000001307 Myosotis scorpioides Species 0.000 title abstract 3
- 239000000843 powder Substances 0.000 claims abstract description 155
- 240000008397 Ganoderma lucidum Species 0.000 claims abstract description 65
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims abstract description 65
- 241000190633 Cordyceps Species 0.000 claims abstract description 21
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 48
- 241001248610 Ophiocordyceps sinensis Species 0.000 claims description 47
- 238000000855 fermentation Methods 0.000 claims description 43
- 230000004151 fermentation Effects 0.000 claims description 43
- 241000222355 Trametes versicolor Species 0.000 claims description 38
- 239000001963 growth medium Substances 0.000 claims description 30
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 24
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 24
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 22
- 239000008103 glucose Substances 0.000 claims description 22
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 20
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 20
- 238000010899 nucleation Methods 0.000 claims description 18
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 13
- 239000001888 Peptone Substances 0.000 claims description 12
- 108010080698 Peptones Proteins 0.000 claims description 12
- 239000002518 antifoaming agent Substances 0.000 claims description 12
- 238000011081 inoculation Methods 0.000 claims description 12
- 235000019319 peptone Nutrition 0.000 claims description 12
- 238000012807 shake-flask culturing Methods 0.000 claims description 12
- 238000009423 ventilation Methods 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 11
- 244000068988 Glycine max Species 0.000 claims description 10
- 235000010469 Glycine max Nutrition 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 9
- 150000004676 glycans Chemical class 0.000 claims description 9
- 229920001282 polysaccharide Polymers 0.000 claims description 9
- 239000005017 polysaccharide Substances 0.000 claims description 9
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 7
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
- 244000061456 Solanum tuberosum Species 0.000 claims description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 238000004062 sedimentation Methods 0.000 claims description 6
- 238000007873 sieving Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- 238000001291 vacuum drying Methods 0.000 claims description 5
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 4
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 4
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 3
- 238000000643 oven drying Methods 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 241000255789 Bombyx mori Species 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 2
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 2
- 239000002775 capsule Substances 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 238000002347 injection Methods 0.000 claims description 2
- 239000007924 injection Substances 0.000 claims description 2
- 239000003826 tablet Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 claims 2
- 238000003756 stirring Methods 0.000 claims 1
- 239000005720 sucrose Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 13
- 230000036039 immunity Effects 0.000 abstract description 7
- 241000894006 Bacteria Species 0.000 abstract 1
- 230000000118 anti-neoplastic effect Effects 0.000 abstract 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 7
- 241000699670 Mus sp. Species 0.000 description 6
- 210000001541 thymus gland Anatomy 0.000 description 6
- 210000004698 lymphocyte Anatomy 0.000 description 4
- 210000002540 macrophage Anatomy 0.000 description 4
- 230000035755 proliferation Effects 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000000242 pagocytic effect Effects 0.000 description 3
- 206010057249 Phagocytosis Diseases 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000004089 microcirculation Effects 0.000 description 2
- 230000008782 phagocytosis Effects 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 241001416980 Paecilomyces hepiali Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011812 mixed powder Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 235000019643 salty taste Nutrition 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 235000019614 sour taste Nutrition 0.000 description 1
- 230000003393 splenic effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/066—Clavicipitaceae
- A61K36/068—Cordyceps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of compound Chinese caterpillar fungus-lucid ganoderma composition and preparation method thereof, in said composition, the weight portion of each composition is: cordyceps 100-300 weight portion, ganoderma lucidum powder 100-220 weight portion, manyzoned polypore bacteria powder 100-220 weight portion.Compound Chinese caterpillar fungus-lucid ganoderma composition of the present invention can significantly rise unit and consolidate, and increases immunity of organisms, has the effect of adjunct antineoplastic.
Description
Technical Field
The invention relates to a compound cordyceps and lucid ganoderma composition and a preparation method thereof, belonging to the field of biological medicine.
Background
At present, health care products in the market are various and mostly have the effects of improving immunity, resisting oxidation, resisting aging and the like, but chemical preparations are added into the health care products for enhancing the drug effect, so that the health care products are not harmful to people after being taken for a short time, but have various side effects after being taken for a long time.
Disclosure of Invention
The invention aims to provide the compound cordyceps and lucid ganoderma composition which has a simple formula and low dosage and can obviously improve the immunity of a human body, improve microcirculation and delay senility.
The purpose of the invention is realized by the following modes:
the compound cordyceps and lucid ganoderma composition is characterized by comprising the following components in parts by weight:
100 plus 300 parts of ganoderma lucidum powder (also called ganoderma lucidum mycelium powder) and 220 parts of coriolus versicolor powder.
Preferably, the composition comprises the following components in parts by weight:
100 portions of cordyceps sinensis powder, 250 portions of ganoderma lucidum powder, 150 portions of ganoderma lucidum powder, 220 portions of coriolus versicolor powder, 100 portions of coriolus versicolor powder and 200 portions of ganoderma lucidum powder.
Preferably, the composition comprises the following components in parts by weight:
100 portions of cordyceps sinensis powder, 100 portions of ganoderma lucidum powder, 200 portions of coriolus versicolor powder, 150 portions of coriolus versicolor powder, 220 portions of ganoderma lucidum powder.
Preferably, the composition comprises the following components in parts by weight:
100 portions of cordyceps sinensis powder, 100 portions of ganoderma lucidum powder, 200 portions of coriolus versicolor powder, 100 portions of coriolus versicolor powder, 220 portions of ganoderma lucidum powder.
The cordyceps polysaccharide in the cordyceps sinensis powder is more than or equal to 1.5%, the ganoderma polysaccharide in the ganoderma lucidum powder is more than or equal to 1.5%, and the ganoderma polysaccharide in the ganoderma lucidum powder is more than or equal to 4.0%.
The preparation method of the compound cordyceps and lucid ganoderma composition comprises the following steps: mixing Cordyceps powder, Ganoderma powder, and Coriolus versicolor powder at a certain proportion.
The cordyceps sinensis powder, the ganoderma lucidum powder and the coriolus versicolor powder used in the invention can be obtained commercially or by self-preparation.
The ganoderma lucidum powder is brown yellow to brown powder, has special fragrance, sour and salty taste, slight bitter taste and no peculiar smell, and the ganoderma lucidum polysaccharide is more than or equal to 1.5g/100 g. Cordyceps polysaccharide in the cordyceps sinensis powder is more than or equal to 1.5%, and Coriolus versicolor polysaccharide in the Coriolus versicolor powder is more than or equal to 4.0%. The above materials can also be prepared by oven drying and pulverizing mycelium obtained by submerged fermentation.
The compound cordyceps and lucid ganoderma composition can be prepared into preparations with pharmaceutically acceptable auxiliary materials, such as: powder, granule, capsule, tablet, oral liquid or injection, etc. The compound cordyceps and lucid ganoderma composition can effectively improve the immunity of a human body and improve microcirculation, has the effects of resisting atherosclerosis, resisting aging and the like, can be used for preparing a medicine for improving the immunity of the organism, and can also be used for preparing a medicine with the auxiliary anti-tumor effect. Compared with single component or any combination of cordyceps sinensis powder, ganoderma lucidum powder and coriolus versicolor powder, the composition can obviously reduce the dosage.
The invention is further illustrated by the following specific test examples:
compared with the single cordyceps sinensis powder and the ganoderma lucidum powder with the same dosage, the compound cordyceps sinensis and ganoderma lucidum mixed powder has significant difference in immunity enhancement, and the results are shown in tables 1, 2 and 3.
Test example 1: effect on mouse thymus index (mean thymus mass mg/mean body mass g)
The experiment is divided into 7 groups, each group comprises 20 mice, wherein a blank group is fed by basic feed, a pure cordyceps sinensis powder group is fed by the basic feed and pure cordyceps sinensis powder (2 g/d), a pure ganoderma lucidum powder group is fed by the basic feed and the pure ganoderma lucidum powder (2 g/d), example 2 is fed by the basic feed and the formula (2 g/d) in example 2, example 4 is fed by the basic feed and the formula (2 g/d) in example 4, example 6 is fed by the basic feed and the formula (2 g/d) in example 6, example 8 is fed by the basic feed and the formula (2 g/d) in example 8, the feeding time is 10d, and the thymus index is detected after 10 d.
TABLE 1
| Group of | Number of animals (only) | Index of thymus | P value |
| Blank group | 20 | 14.13±1.07 | / |
| Pure cordyceps sinensis powder group | 20 | 22.87±5.25 | ＜0.05 |
| Pure ganoderma lucidum powder group | 20 | 23.18±6.92 | ＜0.05 |
| Example 2 | 20 | 33.02±8.46 | ＜0.05 |
| Example 4 | 20 | 34.18±7.95 | ＜0.05 |
| Example 6 | 20 | 32.92±7.67 | ＜0.05 |
| Example 8 | 20 | 35.32±9.19 | ＜0.05 |
And (4) conclusion: from the above table, it can be seen that compared with the blank group, the single cordyceps sinensis powder and the ganoderma lucidum powder have obvious effect of increasing the thymus index of the mice; compared with the pure cordyceps sinensis powder and the ganoderma lucidum powder, the compound cordyceps sinensis and ganoderma lucidum powder has significant difference on the increase of the thymus index of the mouse, and has significant effect.
Test example 2: effect on mouse spleen lymphocyte proliferation
The experiment is divided into 7 groups, each group comprises 20 mice, wherein a blank group is fed by basic feed, a pure cordyceps sinensis powder group is fed by the basic feed and pure cordyceps sinensis powder (2 g/d), a pure ganoderma lucidum powder group is fed by the basic feed and the pure ganoderma lucidum powder (2 g/d), example 1 is fed by the basic feed and the formula (2 g/d) in example 1, example 3 is fed by the basic feed and the formula (2 g/d) in example 3, example 5 is fed by the basic feed and the formula (2 g/d) in example 5, example 7 is fed by the basic feed and the formula (2 g/d) in example 7, the feeding time is 10d, and the lymphocyte proliferation condition is detected after 10 d.
TABLE 2
| Group of | Number of animals (only) | Difference in optical density | P value |
| Blank group | 20 | 0.0517±0.0235 | / |
| Pure cordyceps sinensis powder group | 20 | 0.2753±0.0897 | ＜0.05 |
| Pure ganoderma lucidum powder group | 20 | 0.2688±0.0769 | ＜0.05 |
| Example 1 | 20 | 0.5047±0.0572 | ＜0.05 |
| Example 3 | 20 | 0.5381±0.0497 | ＜0.05 |
| Example 5 | 20 | 0.5649±0.0539 | ＜0.05 |
| Example 7 | 20 | 0.6014±0.0329 | ＜0.05 |
And (4) conclusion: from the above table, it can be seen that compared with the blank group, the pure cordyceps sinensis powder and the ganoderma lucidum powder have significant effect on the mouse spleen lymphocyte proliferation; compared with the pure cordyceps sinensis powder and the ganoderma lucidum powder, the compound cordyceps sinensis and ganoderma lucidum powder has obvious effect on the proliferation of splenic lymphocytes of mice.
Test example 3: effect on phagocytic Capacity of mouse macrophages
The experiment is divided into 7 groups, each group comprises 20 mice, wherein a blank group is fed by basic feed, a pure cordyceps sinensis powder group is fed by the basic feed and pure cordyceps sinensis powder (2 g/d), a pure ganoderma lucidum powder group is fed by the basic feed and the pure ganoderma lucidum powder (2 g/d), example 2 is fed by the basic feed and the formula (2 g/d) in example 2, example 3 is fed by the basic feed and the formula (2 g/d) in example 3, example 4 is fed by the basic feed and the formula (2 g/d) in example 4, example 9 is fed by the basic feed and the formula (2 g/d) in example 9, the feeding time is 10d, and the macrophage phagocytosis index of the mice is detected after 10 d.
TABLE 3
| Group of | Number of animals (only) | Phagocytic index | P value |
| Blank group | 20 | 22.54±7.62 | / |
| Pure cordyceps sinensis powder group | 20 | 30.72±8.91 | ＜0.05 |
| Pure ganoderma lucidum powder group | 20 | 32.15±7.39 | ＜0.05 |
| Example 2 | 20 | 35.38±5.64 | ＜0.05 |
| Example 3 | 20 | 35.29±4.39 | ＜0.05 |
| Example 4 | 20 | 36.64±5.86 | ＜0.05 |
| Example 9 | 20 | 37.09±4.85 | ＜0.05 |
And (4) conclusion: from the above table, it can be seen that, compared with the blank group, the cordyceps sinensis powder and the ganoderma lucidum powder have significant phagocytic effect on mouse macrophages; compared with pure cordyceps sinensis powder and ganoderma lucidum powder, the compound cordyceps sinensis and ganoderma lucidum powder has obvious phagocytosis effect on mouse macrophages.
Therefore, the composition for enhancing the immunity of the organism and having the effect of assisting the anti-tumor is not the simple addition of the functions of all the components and is the synergistic effect of all the components. In addition, the composition of the invention is placed for about 6 months at normal temperature, and the content of the effective components is basically unchanged.
The specific implementation mode is as follows:
the invention is further illustrated by the following specific examples. However, the specific details of the embodiments are merely for explaining the present invention and should not be construed as limiting the general technical solution of the present invention.
The deep fermentation process of the cordyceps sinensis powder liquid comprises the following steps:
the strain for cordyceps sinensis powder comprises the following strains: cordyceps sinensis-Paecilomyces hepiali Chen (Paecilomyces hepiali) Cs-4, purchased from the center for the culture Collection of microorganisms of the academy of sciences of China.
The preserved strain taken out of the refrigerator is inoculated on a fresh slant culture medium, and the formula of the culture medium is as follows: potato 8.0%, glucose 3.0%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.15%, agar 1.3%, and culturing at 26 deg.C for 4 days.
Transferring the strain cultured on the slant into a shake flask, wherein the shake flask culture medium is as follows: glucose 2%, peptone 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, cultured at 26 ℃ for 2 days.
After the shake flask culture is finished, transferring the strain into a seeding tank, wherein the culture medium of the seeding tank is as follows: 2% of glucose, 1% of peptone, 0.1% of potassium dihydrogen phosphate, 0.05% of magnesium sulfate and 0.1% of defoaming agent, and the fermentation process conditions are as follows: the inoculation amount is 10 percent, the temperature is 26 +/-1 ℃, the ventilation volume is 1: 0.5vvm, and the tank pressure is 0.03 MPa.
The seeding tank is switched to a large tank for fermentation after 3 days of fermentation, and the large tank culture medium is as follows: 1.5 percent of glucose, 2 percent of peptone, 0.05 percent of potassium dihydrogen phosphate, 0.07 percent of magnesium sulfate and 0.1 percent of defoaming agent. The fermentation process comprises the following steps: the inoculation amount is 10 percent, the culture temperature is 26 +/-1 ℃, the ventilation rate is 1: 0.5vvm, and the tank pressure is 0.03 MPa.
After fermentation, separating the fermentation liquor by a horizontal spiral discharge sedimentation centrifuge, vacuum drying mycelium at the temperature of below 70 ℃, drying, crushing and sieving by a 100-mesh sieve to obtain the cordyceps sinensis powder.
The ganoderma lucidum powder liquid submerged fermentation process comprises the following steps:
the strains for the ganoderma lucidum powder: ganoderma lucidum [ Ganoderma lucidum (Curtis: Fr) P.Karst ], purchased from the center for the culture Collection of microorganisms of the academy of sciences of China.
The preserved strain taken out of the refrigerator is inoculated on a fresh slant culture medium, and the formula of the culture medium is as follows: potato 8%, glucose 2.0%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.08%, agar 1.5%, and culturing at 25 deg.C for 3 days.
Transferring the strain cultured on the slant into a shake flask, wherein the shake flask culture medium is as follows: 1.0 percent of soybean cake powder, 3.0 percent of cane sugar, 0.15 percent of monopotassium phosphate, 0.075 percent of magnesium sulfate and 0.05 percent of ammonium sulfate, and culturing for 2 days at 25 ℃.
After the shake flask culture is finished, transferring the strain into a seeding tank, wherein the culture medium of the seeding tank is as follows: 1% of soybean cake powder, 2% of glucose, 0.1% of monopotassium phosphate, 0.05% of magnesium sulfate and 0.1% of defoaming agent, wherein the fermentation process conditions are as follows: the inoculation amount is 10 percent, the temperature is 26 +/-1 ℃, the ventilation volume is 1: 0.5vvm, and the tank pressure is 0.03 MPa.
The seeding tank is switched to a large tank for fermentation after 2 days of fermentation, and the large tank culture medium is as follows: 2.0 percent of soybean cake powder, 0.5 percent of silkworm chrysalis powder, 1 percent of glucose, 0.08 percent of monopotassium phosphate, 0.07 percent of magnesium sulfate and 0.1 percent of defoaming agent. The fermentation process comprises the following steps: the inoculation amount is 10 percent, the culture temperature is 27 +/-1 ℃, the ventilation rate is 1: 0.5vvm, and the tank pressure is 0.03 MPa.
After fermentation, separating the fermentation liquor by a horizontal spiral discharge sedimentation centrifuge, vacuum drying mycelium at the temperature of below 70 ℃, drying, crushing and sieving by a 100-mesh sieve to obtain the ganoderma lucidum powder.
The submerged fermentation process of the coriolus versicolor powder liquid comprises the following steps:
the strain for the coriolus versicolor bacterial powder comprises the following strains: coriolus Versicolor (Polystictus Versicolor (L) Fr) is purchased from the center for the preservation of microbial strains of the Chinese academy of sciences.
The preserved strain taken out of the refrigerator is inoculated on a fresh slant culture medium, and the formula of the culture medium is as follows: 10.0 percent of potato, 2.0 percent of glucose, 0.3 percent of monopotassium phosphate, 0.15 percent of magnesium sulfate and 1.3 percent of agar are cultured for 5 days at 26 ℃.
Transferring the strain cultured on the slant into a shake flask, wherein the shake flask culture medium is as follows: 1.0% of soybean cake powder, 2% of glucose, 0.5% of peptone, 0.1% of potassium dihydrogen phosphate and 0.05% of magnesium sulfate, and culturing for 3 days at 26 ℃.
After the shake flask culture is finished, transferring the strain into a seeding tank, wherein the culture medium of the seeding tank is as follows: 2% of glucose, 1% of peptone, 0.1% of sodium dihydrogen phosphate, 0.05% of magnesium sulfate and 0.1% of defoaming agent, wherein the fermentation process conditions are as follows: the inoculation amount is 10 percent, the temperature is 26 +/-1 ℃, the ventilation volume is 1: 0.5vvm, and the tank pressure is 0.03 MPa.
The seeding tank is switched to a large tank for fermentation after 3 days of fermentation, and the large tank culture medium is as follows: 2% of glucose, 2% of soybean cake powder, 1% of peptone, 0.4% of sodium dihydrogen phosphate, 0.05% of magnesium sulfate and 0.1% of defoaming agent, and the fermentation process comprises the following steps: the inoculation amount is 15 percent, the culture temperature is 26 +/-1 ℃, and the ventilation volume is 1: 0.5 vvm; the pot pressure is 0.03 MPa.
After fermentation, separating the fermentation liquid by horizontal spiral discharge sedimentation centrifuge, vacuum drying mycelium at below 70 deg.C, oven drying, pulverizing, and sieving with 100 mesh sieve to obtain Coriolus versicolor powder.
The cordyceps sinensis powder, the ganoderma lucidum powder and the coriolus versicolor powder have the following raw material quality indexes:
example 1
100mg of cordyceps sinensis powder, 220mg of ganoderma lucidum powder and 180mg of coriolus versicolor powder; mixing the three components.
Example 2
100mg of cordyceps sinensis powder, 180mg of ganoderma lucidum powder and 220mg of coriolus versicolor powder; mixing the three components.
Example 3
150mg of cordyceps sinensis powder, 150mg of ganoderma lucidum powder and 200mg of coriolus versicolor powder; mixing the three components.
Example 4
150mg of cordyceps sinensis powder, 200mg of ganoderma lucidum powder and 150mg of coriolus versicolor powder; mixing the three components.
Example 5
200mg of cordyceps sinensis powder, 150mg of ganoderma lucidum powder and 150mg of coriolus versicolor powder; mixing the three components.
Example 6
250mg of cordyceps sinensis powder, 100mg of ganoderma lucidum powder and 150mg of coriolus versicolor powder; mixing the three components.
Example 7
250mg of cordyceps sinensis powder, 150mg of ganoderma lucidum powder and 100mg of coriolus versicolor powder; mixing the three components.
Example 8
250mg of cordyceps sinensis powder, 100mg of ganoderma lucidum powder and 150mg of coriolus versicolor powder; mixing the three components.
Example 9
300mg of cordyceps sinensis powder, 100mg of ganoderma lucidum powder and 100mg of coriolus versicolor powder; mixing the three components.
Claims (6)
1. A preparation method of a compound cordyceps and lucid ganoderma composition is characterized by mixing and stirring the components uniformly: the components are as follows:
100 portions of cordyceps sinensis powder, 100 portions of ganoderma lucidum powder, 220 portions of coriolus versicolor powder, 100 portions of coriolus versicolor powder, 220 portions of ganoderma lucidum powder; cordyceps polysaccharide in the cordyceps sinensis powder is more than or equal to 1.5 percent, ganoderma polysaccharide in the ganoderma lucidum powder is more than or equal to 1.5 percent, and ganoderma lucidum polysaccharide in the ganoderma lucidum powder is more than or equal to 4.0 percent; wherein,
the submerged fermentation process of the cordyceps sinensis powder liquid comprises the following steps:
taking cordyceps sinensis-paecilomyces hepiali Cs-4 strain to inoculate on a fresh slant culture medium, wherein the formula of the culture medium is as follows: 8.0 percent of potato, 3.0 percent of glucose, 0.3 percent of monopotassium phosphate, 0.15 percent of magnesium sulfate and 1.3 percent of agar are cultured for 4 days at 26 ℃; transferring the strain cultured on the slant into a shake flask, wherein the shake flask culture medium is as follows: 2% of glucose, 1% of peptone, 0.1% of potassium dihydrogen phosphate and 0.05% of magnesium sulfate, and culturing at 26 ℃ for 2 days; after the shake flask culture is finished, transferring the strain into a seeding tank, wherein the culture medium of the seeding tank is as follows: 2% of glucose, 1% of peptone, 0.1% of potassium dihydrogen phosphate, 0.05% of magnesium sulfate and 0.1% of defoaming agent, and the fermentation process conditions are as follows: the inoculation amount is 10 percent, the temperature is 26 +/-1 ℃, the ventilation volume is 1: 0.5vvm, and the tank pressure is 0.03 MPa; the seeding tank is switched to a large tank for fermentation after 3 days of fermentation, and the large tank culture medium is as follows: 1.5 percent of glucose, 2 percent of peptone, 0.05 percent of potassium dihydrogen phosphate, 0.07 percent of magnesium sulfate and 0.1 percent of defoaming agent, and the fermentation process comprises the following steps: the inoculation amount is 10 percent, the culture temperature is 26 +/-1 ℃, the ventilation rate is 1: 0.5vvm, and the tank pressure is 0.03 MPa; after fermentation, separating the fermentation liquor by a horizontal spiral discharge sedimentation centrifuge, drying mycelium under vacuum at the temperature of below 70 ℃, crushing after drying, and sieving with a 100-mesh sieve to obtain cordyceps sinensis powder;
the ganoderma lucidum powder liquid submerged fermentation process comprises the following steps:
inoculating the lucid ganoderma strain on a fresh slant culture medium, wherein the formula of the culture medium is as follows: 8% of potato, 2.0% of glucose, 0.2% of potassium dihydrogen phosphate, 0.08% of magnesium sulfate and 1.5% of agar, and culturing for 3 days at 25 ℃; transferring the strain cultured on the slant into a shake flask, wherein the shake flask culture medium is as follows: 1.0% of soybean cake powder, 3.0% of sucrose, 0.15% of potassium dihydrogen phosphate, 0.075% of magnesium sulfate and 0.05% of ammonium sulfate, and culturing for 2 days at 25 ℃; after the shake flask culture is finished, transferring the strain into a seeding tank, wherein the culture medium of the seeding tank is as follows: 1% of soybean cake powder, 2% of glucose, 0.1% of monopotassium phosphate, 0.05% of magnesium sulfate and 0.1% of defoaming agent, wherein the fermentation process conditions are as follows: the inoculation amount is 10 percent, the temperature is 26 +/-1 ℃, the ventilation volume is 1: 0.5vvm, and the tank pressure is 0.03 MPa; the seeding tank is switched to a large tank for fermentation after 2 days of fermentation, and the large tank culture medium is as follows: 2.0% of soybean cake powder, 0.5% of silkworm chrysalis powder, 1% of glucose, 0.08% of potassium dihydrogen phosphate, 0.07% of magnesium sulfate and 0.1% of defoaming agent, and the fermentation process comprises the following steps: the inoculation amount is 10 percent, the culture temperature is 27 +/-1 ℃, the ventilation rate is 1: 0.5vvm, and the tank pressure is 0.03 MPa; after fermentation, separating the fermentation liquor by a horizontal spiral discharge sedimentation centrifuge, vacuum drying mycelium at the temperature of below 70 ℃, drying, crushing and sieving by a 100-mesh sieve to obtain ganoderma lucidum powder;
the submerged fermentation process of Coriolus versicolor powder liquid comprises the following steps:
inoculating coriolus versicolor strains on a fresh slant culture medium, wherein the formula of the culture medium is as follows: 10.0% of potato, 2.0% of glucose, 0.3% of monopotassium phosphate, 0.15% of magnesium sulfate and 1.3% of agar, and culturing for 5 days at 26 ℃; transferring the strain cultured on the slant into a shake flask, wherein the shake flask culture medium is as follows: 1.0% of soybean cake powder, 2% of glucose, 0.5% of peptone, 0.1% of potassium dihydrogen phosphate and 0.05% of magnesium sulfate, and culturing for 3 days at 26 ℃; after the shake flask culture is finished, transferring the strain into a seeding tank, wherein the culture medium of the seeding tank is as follows: 2% of glucose, 1% of peptone, 0.1% of sodium dihydrogen phosphate, 0.05% of magnesium sulfate and 0.1% of defoaming agent, wherein the fermentation process conditions are as follows: the inoculation amount is 10 percent, the temperature is 26 +/-1 ℃, the ventilation volume is 1: 0.5vvm, and the tank pressure is 0.03 MPa; the seeding tank is switched to a large tank for fermentation after 3 days of fermentation, and the large tank culture medium is as follows: 2% of glucose, 2% of soybean cake powder, 1% of peptone, 0.4% of sodium dihydrogen phosphate, 0.05% of magnesium sulfate and 0.1% of defoaming agent, and the fermentation process comprises the following steps: the inoculation amount is 15 percent, the culture temperature is 26 +/-1 ℃, and the ventilation volume is 1: 0.5 vvm; the tank pressure is 0.03 MPa; after fermentation, separating the fermentation liquid by horizontal spiral discharge sedimentation centrifuge, vacuum drying mycelium at below 70 deg.C, oven drying, pulverizing, and sieving with 100 mesh sieve to obtain Coriolus versicolor powder.
2. The preparation method of the compound cordyceps and ganoderma composition according to claim 1, which is characterized by comprising the following components in parts by weight: 100 portions of cordyceps sinensis powder, 250 portions of ganoderma lucidum powder, 150 portions of ganoderma lucidum powder, 220 portions of coriolus versicolor powder, 100 portions of coriolus versicolor powder and 200 portions of ganoderma lucidum powder.
3. The preparation method of the compound cordyceps and ganoderma composition according to claim 1, which is characterized by comprising the following components in parts by weight: 100 portions of cordyceps sinensis powder, 100 portions of ganoderma lucidum powder, 200 portions of coriolus versicolor powder, 150 portions of coriolus versicolor powder, 220 portions of ganoderma lucidum powder.
4. The preparation method of the compound cordyceps and ganoderma composition according to claim 1, which is characterized by comprising the following components in parts by weight: 100 portions of cordyceps sinensis powder, 100 portions of ganoderma lucidum powder, 200 portions of coriolus versicolor powder, 100 portions of coriolus versicolor powder, 220 portions of ganoderma lucidum powder.
5. The method for preparing the compound cordyceps and ganoderma composition according to claim 1, 2, 3 or 4, wherein the compound cordyceps and ganoderma composition is prepared into a preparation together with pharmaceutically acceptable auxiliary materials.
6. The method for preparing the compound cordyceps and ganoderma composition according to claim 5, wherein the preparation is powder, granules, capsules, tablets, oral liquid or injection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310507737.8A CN103549386B (en) | 2013-10-24 | 2013-10-24 | A kind of compound Chinese caterpillar fungus-lucid ganoderma composition and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310507737.8A CN103549386B (en) | 2013-10-24 | 2013-10-24 | A kind of compound Chinese caterpillar fungus-lucid ganoderma composition and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103549386A CN103549386A (en) | 2014-02-05 |
| CN103549386B true CN103549386B (en) | 2015-09-16 |
Family
ID=50003893
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310507737.8A Active CN103549386B (en) | 2013-10-24 | 2013-10-24 | A kind of compound Chinese caterpillar fungus-lucid ganoderma composition and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103549386B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104905262B (en) * | 2015-05-29 | 2017-12-22 | 湖北省农业科学院农产品加工与核农技术研究所 | A kind of method for preparing Cordyceps-Ganoderma compound formulation |
| CN104997815A (en) * | 2015-07-08 | 2015-10-28 | 哈尔滨瑞康源生物科技有限公司 | Cordyceps sinensis and Mythic Fungus fermentation powder composite lozenge and preparation method thereof |
| CN107184615A (en) * | 2017-07-12 | 2017-09-22 | 亳州学院 | A kind of adjuvant Chinese medicine composition and preparation method thereof |
| CN108077904A (en) * | 2017-12-21 | 2018-05-29 | 山西佰富康生物科技有限公司 | A kind of Cordyceps militaris nutrition brewed powder and preparation method thereof |
| CN115873718B (en) * | 2022-10-25 | 2024-08-16 | 江苏神华药业有限公司 | Liquid fermentation medium for armillaria mellea mycelium and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW200920387A (en) * | 2007-11-14 | 2009-05-16 | Shiu-Nan Chen | Composition of mushroom beta-glucan for anti-cancer and preparation method thereof |
| CN101579365A (en) * | 2008-12-05 | 2009-11-18 | 王星丽 | Edible fungus tumor health capsule and preparation method thereof |
| CN101766644A (en) * | 2008-12-31 | 2010-07-07 | 陈秀男 | Mushroom polysaccharide composition with anticancer effect and preparation method thereof |
| EP2522356A2 (en) * | 2009-10-02 | 2012-11-14 | Pontificia Universidad Javeriana | Bioactive fraction of petiveria alliacea, pharmaceutical composition containing same, and combination with immunostimulants for treating cancer |
-
2013
- 2013-10-24 CN CN201310507737.8A patent/CN103549386B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW200920387A (en) * | 2007-11-14 | 2009-05-16 | Shiu-Nan Chen | Composition of mushroom beta-glucan for anti-cancer and preparation method thereof |
| CN101579365A (en) * | 2008-12-05 | 2009-11-18 | 王星丽 | Edible fungus tumor health capsule and preparation method thereof |
| CN101766644A (en) * | 2008-12-31 | 2010-07-07 | 陈秀男 | Mushroom polysaccharide composition with anticancer effect and preparation method thereof |
| EP2522356A2 (en) * | 2009-10-02 | 2012-11-14 | Pontificia Universidad Javeriana | Bioactive fraction of petiveria alliacea, pharmaceutical composition containing same, and combination with immunostimulants for treating cancer |
Non-Patent Citations (1)
| Title |
|---|
| 真菌多糖与人体健康;周小鹭;《黑龙江生态工程职业学院学报》;20090930;第22卷(第5期);第72-74页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103549386A (en) | 2014-02-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103549386B (en) | A kind of compound Chinese caterpillar fungus-lucid ganoderma composition and preparation method thereof | |
| CN1358413A (en) | Method for preparing mushroom mycelium and use | |
| CN105820956B (en) | A strain of Antrodia camphorata and liquid fermentation method of Antrodia camphorata | |
| CN111803533A (en) | Composition for reducing blood sugar and blood fat, preparation method and application thereof | |
| CN102816806A (en) | Production method of grifolan selenium compound | |
| CN106047978B (en) | A method for synthesizing rare ginsenoside CK by biotransformation of natural saponins | |
| CN105368695B (en) | A kind of Poria cocos propolis fermented vinegar and preparation method thereof | |
| KR101420392B1 (en) | Eel feed and feed additive for energy savings of aquaculture | |
| KR20180093488A (en) | Method for manufacturing JuksongwonCompuond-P through frement | |
| CN100438882C (en) | A kind of preparation method of Sanhuang health-care oral liquid | |
| CN102960711B (en) | Natural nourishment and preparation method thereof | |
| CN104718986A (en) | Cordyceps sinensis Chinese yam | |
| CN105586269B (en) | The method for producing maitake mushroom mycelia raw material using mutagenesis Grifola frondosa strain | |
| CN107916229B (en) | One plant of Inonotus obliquus and its application | |
| JP5081485B2 (en) | Anticancer agent and method for producing anticancer agent | |
| CN103876014B (en) | A kind of compound phellinus oral liquid and preparation method thereof | |
| CN114854604A (en) | Ganoderma lucidum, high-concentration oral liquid containing ganoderma lucidum and preparation method of oral liquid | |
| CN104208106A (en) | Antrodia camphorate extract having human body immunity enhancing function, and its application | |
| CN110387333A (en) | A method for cultivating Antrodia camphorata with Lanping Cordyceps fungus powder | |
| KR102118731B1 (en) | Composition comprising betaglucan as an effective ingredient for relieving hangover or preventing, improving or treating alcoholic liver disease | |
| CN104686199A (en) | Cordyceps barley | |
| CN104719782A (en) | Cordyceps sinensis mung beans | |
| CN104643031B (en) | A kind of ferment cordyceps sinensis source property beta glucan extract and preparation method thereof | |
| CN1314712C (en) | Preparation for fungus extracellular polysaccharide compound and application thereof | |
| CN102816807A (en) | Production method of grifolan manganese compound |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |