CN103446312A - Compound poplar flower oral liquid and preparation method thereof - Google Patents
Compound poplar flower oral liquid and preparation method thereof Download PDFInfo
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Abstract
The invention discloses compound poplar flower oral liquid and a preparation method thereof. The compound poplar flower oral liquid comprises the following active components in parts by mass: 50 to 100 parts of poplar flower extract, 30 to 80 parts of sophora angustifolia root extract, 20 to 60 parts of anemone root extract and 5 to 15 parts of berberine sulfate, wherein the poplar flower extract, the sophora angustifolia root extract and the anemone root extract are respectively prepared by the supercritical extraction CO2 extraction method with dried poplar flower, sophora angustifolia root and anemone root as raw materials. The pure compound poplar flower oral liquid, provided by the invention, has the effects of clearing heat and removing toxicity, resolving dampness and curing durchfall, also has antibacterial and insecticidal effects, can be used for treating dysentery of livestock and poultry an various enteritis and diarrhea, can treat both symptoms and root causes, and has definite curative effect and high safety.
Description
Technical field
The invention belongs to the veterinary Chinese medicine technical field, relate to a kind of compound poplar flower oral liquid and preparation method thereof.
Background technology
Fast development along with modern farming, chemical drugs is excessively used the drug resistance and the drug residue problem that cause more and more obvious in the past for a long time, simultaneously due to social development, people are also improving gradually to the requirement of health diet, and the medicinal safety of animal product is also produced to increasing worry." fast-growing chicken " event in 2012, even caused especially the concern of whole industry entire society to chemical drugs for animals.Current weightening finish fast the modern times cultivation require under, the gastrointestinal tract burden, the anti-inflammation of chemical drugs for animals can not meet production requirement, only have Chinese medicine to have natural sex, noresidue, have no drug resistance and the characteristics such as comprehensively regulating, its increasing use in animal produces is the inexorable trend of modern cultivation development.
Summary of the invention
The problem that the present invention solves is to provide a kind of compound poplar flower oral liquid and preparation method thereof, and the Chinese traditional compound medicine that this oral liquid is pure Chinese medicine, to the dysentery of poultry and various enteritis, diarrhoea, treating both the principal and secondary aspects of a disease, determined curative effect, have advantage safe, practical and convenient, green noresidue.
The present invention is achieved through the following technical solutions:
A kind of compound poplar flower oral liquid, in mass fraction, its effective ingredient comprises following component:
The poplar flower extract of 50～100 parts, the Radix Sophorae Flavescentis extract of 30～80 parts, the berberine sulfate of the Radix Pulsatillae extract of 20～60 parts and 5～15 parts;
Wherein, poplar flower extract, Radix Sophorae Flavescentis extract, Radix Pulsatillae extract are respectively to using dry flower of poplar, Radix Sophorae Flavescentis, the Radix Pulsatillae as raw material, adopt supercritical CO
2prepared by extraction.
In mass fraction, its effective ingredient comprises following component:
The poplar flower extract of 60～80 parts, the Radix Sophorae Flavescentis extract of 40～60 parts, the berberine sulfate of the Radix Pulsatillae extract of 20～30 parts and 5～10 parts.
In mass fraction, its effective ingredient comprises following component:
The poplar flower extract of 60 parts, the Radix Sophorae Flavescentis extract of 40 parts, the berberine sulfate of the Radix Pulsatillae extract of 20 parts and 10 parts.
In mass fraction, also comprise following component in compound poplar flower oral liquid:
The sodium benzoate of mass concentration 0.1～2.0%, and volumetric concentration is respectively 0.5～2.5% benzyl alcohol, 3.0～5.0% glycerin methylal, 1.0～3.0% propylene glycol and 1.0～3.0% Tween 80.
Described 1ml compound poplar flower oral liquid is equivalent to the primary crude drug of 1.35～1.5g.
Being prepared as of described poplar flower extract, Radix Sophorae Flavescentis extract, Radix Pulsatillae extract:
To pulverize, sieve through the flower of poplar of super-dry, use supercritical CO
2extraction preparation: extracting pressure 30Mpa, 45 ℃ of extraction temperature, extraction CO
2flow 40kg/h, once reinforced, the one-level extraction, the second-order separation, extract 1.5 hours and collect extract once, every 30 minutes, collects once later; Extract 3 hours, merging filtrate, filter; 60 ℃ of vacuum dryings, make the poplar flower extract that every 1 gram is equivalent to 20 gram crude drugs;
To pulverize, sieve through the Radix Sophorae Flavescentis of super-dry, by after Radix Sophorae Flavescentis powder and ammonia mixing, soak 12～24 hours, use supercritical CO
2extraction preparation: extracting pressure 35Mpa, 35 ℃ of extraction temperature, extraction CO
2flow 40kg/h, once reinforced, the one-level extraction, the second-order separation, in extraction, the ethanol of minute 3 injection volumetric concentrations 75%, extract 1.5 hours and collect extract once, every 30 minutes, collects once later; Extract 8 hours, merging filtrate, filter; 60 ℃ of vacuum dryings, make the Radix Sophorae Flavescentis extract that every 1 gram is equivalent to 20 gram crude drugs;
To pulverize, sieve through the Radix Pulsatillae of super-dry, use supercritical CO
2extraction preparation: extracting pressure 30Mpa, 45 ℃ of extraction temperature, extraction CO
2flow 40kg/h, once reinforced, the one-level extraction, the second-order separation, extract 1.5 hours and collect extract once, every 30 minutes, collects once later; Extract 3 hours, merging filtrate, filter; 60 ℃ of vacuum dryings, make the Radix Pulsatillae extract that every 1 gram is equivalent to 20 gram crude drugs.
A kind of preparation method of compound poplar flower oral liquid comprises the following steps:
1) organic solvent is heated to 50～70 ℃, in mass fraction, adds respectively the poplar flower extract of 50～100 parts, the Radix Pulsatillae extract of the Radix Sophorae Flavescentis extract of 30～80 parts and 20～60 parts, obtain preliminary effective ingredient solution after fully mixing;
2) in mass fraction, add 5～15 parts of berberine sulfates in preliminary effective ingredient solution, obtain effective ingredient solution after fully mixing;
3) effective ingredient solution is put in emulsion tank, adds antiseptic, stabilizing agent, emulsifying agent, add water to enoughly, fully emulsified 10～30 minutes, obtain compound poplar flower oral liquid.
Described organic solvent is propylene glycol, and described antiseptic comprises sodium benzoate, benzyl alcohol, and described stabilizing agent is glycerin methylal, and described emulsifying agent is Tween 80;
In compound poplar flower oral liquid, the volumetric concentration of propylene glycol is 1.0～3.0%, and the mass concentration of sodium benzoate is 0.1～2.0%, and the volumetric concentration of benzyl alcohol is 0.5～2.5%, the volumetric concentration of glycerin methylal is 3.0～5.0%, and the volumetric concentration of Tween 80 is 1.0～3.0%.
Being prepared as of described poplar flower extract, Radix Sophorae Flavescentis extract, Radix Pulsatillae extract:
To pulverize, sieve through the flower of poplar of super-dry, use supercritical CO
2extraction preparation: extracting pressure 30Mpa, 45 ℃ of extraction temperature, extraction CO
2flow 40kg/h, once reinforced, the one-level extraction, the second-order separation, extract 1.5 hours and collect extract once, every 30 minutes, collects once later; Extract 3 hours, merging filtrate, filter; 60 ℃ of vacuum dryings, make the poplar flower extract that every 1 gram is equivalent to 20 gram crude drugs;
To pulverize, sieve through the Radix Sophorae Flavescentis of super-dry, by after Radix Sophorae Flavescentis powder and ammonia mixing, soak 12～24 hours, use supercritical CO
2extraction preparation: extracting pressure 35Mpa, 35 ℃ of extraction temperature, extraction CO
2flow 40kg/h, once reinforced, the one-level extraction, the second-order separation, in extraction, the ethanol of minute 3 injection volumetric concentrations 75%, extract 1.5 hours and collect extract once, every 30 minutes, collects once later; Extract 8 hours, merging filtrate, filter; 60 ℃ of vacuum dryings, make the Radix Sophorae Flavescentis extract that every 1 gram is equivalent to 20 gram crude drugs;
To pulverize, sieve through the Radix Pulsatillae of super-dry, use supercritical CO
2extraction preparation: extracting pressure 30Mpa, 45 ℃ of extraction temperature, extraction CO
2flow 40kg/h, once reinforced, the one-level extraction, the second-order separation, extract 1.5 hours and collect extract once, every 30 minutes, collects once later; Extract 3 hours, merging filtrate, filter; 60 ℃ of vacuum dryings, make the Radix Pulsatillae extract that every 1 gram is equivalent to 20 gram crude drugs.
Above-mentioned solution is rendered in emulsion tank, adds antiseptic, stabilizing agent, and emulsifying 10-30 minute obtains pure Chinese medicine compound poplar flower oral liquid.
The application of described compound poplar flower oral liquid in the medicine of the dysentery for preparing anti-poultry, enteritis, diarrhoea.
Compared with prior art, the present invention has following useful technique effect:
1, pure Chinese medicine compound poplar flower oral liquid provided by the invention, heat-clearing and toxic substances removing, the removing dampness dysentery relieving, antibiotic insecticidal action, can be used in treatment animal dysentery, various enteritis and diarrhoea, treating both the principal and secondary aspects of a disease, determined curative effect, safe.
The drug residue produced with diarrhoea with antibiotic therapy treatment animal dysentery dysentery, various enteritis, the drug resistance of generation are compared, and safety significantly promotes; It is applied, and can reduce the risk of livestock Fowl meat food product drug residue.
Chinese medicine flower of poplar, Radix Sophorae Flavescentis, Radix Pulsatillae extract and berberine sulfate mainly comprise the materials such as flavone, matrine, saponin, saccharide and alkaloid.These extracts have the effect of heat-clearing and toxic substances removing, relieving diarrhea with astringents, removing dampness dysentery relieving, strengthening the spleen and nourishing the stomach through clinical verification.Pharmacological research proves that these extracts can directly suppress or kill pathogen, improves the resistance of body, the antagonism immunosuppressant, and have no side effect.To the dysentery of poultry and various enteritis, diarrhoea, treating both the principal and secondary aspects of a disease.
2, pure Chinese medicine compound poplar flower oral liquid provided by the invention, adopt highly purified Chinese medicine extract, when ensuring effective constituent, adds stabilizing agent, and adopt emulsifying process, guarantees the stable of finished product character and quality.
3, pure Chinese medicine compound poplar flower oral liquid provided by the invention, for brown to brownish black liquid; Can mix drink and can gavage administration, compare with traditional powder, have easy to use, effect rapidly, bioavailability high etc. characteristics.
4, pure Chinese medicine compound poplar flower oral liquid provided by the invention, to the treatment of animal dysentery, various enteritis and diarrhoea, have the curative effect of working as with the enrofloxacin solution phase; And experimental result shows that this oral liquid can share with the antibiotic such as enrofloxacin, share treatment performance synergism.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail, and the explanation of the invention is not limited.
Pure Chinese medicine compound poplar flower oral liquid provided by the invention, adopt and have heat-clearing and toxic substances removing, and the Chinese prescription of removing dampness dysentery relieving, adopt highly purified Chinese medicine extract, when ensuring effective constituent, adds stabilizing agent, and through emulsifying process; Finally make be convenient to the administration of mixed drink oral liquid formulations.Below in conjunction with concrete prescription and preparation process thereof, and, to the prevention of swine dysentery, the effect experiment for the treatment of, the present invention is described in further detail, and described is explanation of the present invention rather than restriction.
Above-mentioned pure Chinese medicine compound poplar flower oral liquid, its effective ingredient comprises following component:
The poplar flower extract of 50～100 parts, the Radix Sophorae Flavescentis extract of 30～80 parts, the berberine sulfate of the Radix Pulsatillae extract of 20～60 parts and 5～15 parts.
Flower of poplar: bitter in the mouth, cold in nature.Return stomach, large intestine channel.Function cures mainly: heat-clearing and toxic substances removing, relieving diarrhea with astringents, removing dampness dysentery relieving, strengthening the spleen and nourishing the stomach.Clinical practice: clinical have significant curative effect with flower of poplar or its preparation to bacillary dysentery, intestinal tract disease.Poplar flower extract is mainly containing flavone, organic acid, aminoacid, saccharide and alkaloid etc.Pharmacological research: but flower of poplar can directly kill pathogen; Improve the resistance of body, the antagonism immunosuppressant, the immunologic function of regulating body, effectively alleviate the various stress between the poultry period of disease; Animal intestines and stomach flesh and sphincter tone are improved, and the secretion inhibitor function, show the effect of convergence stopping leak.
Radix Sophorae Flavescentis: bitter in the mouth; Cold in nature.Return liver; Kidney; Large intestine; Small intestine meridian; Bladder; Heart channel; Urinary bladder channel.Function cures mainly: heat clearing and damp drying; Wind dispelling insecticide.Clinical practice: extensive use and multiple humidifier fever, as damp-heat dysentery; Discharging fresh blood stool.Be used for the treatment of dysentery in classical prescription, dysentery is more than.Contain matrine, oxymatrine etc. in extract.Pharmacological research: Radix Sophorae Flavescentis has the effect of anti-inflammation, and staphylococcus aureus, escherichia coli are all had to inhibitory action, energy diuresis, antiallergic, analgesia.
The Radix Pulsatillae: bitter in the mouth, cold in nature.Return stomach, large intestine channel.Function cures mainly: have heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, the effect of removing dampness and killing parasites.Clinical practice: pyretic toxicity dysentery is usually used in treating bacillary dysentery, protozoal dysentery.Radix Pulsatillae root is containing anemoside, Anemonin etc.Pharmacological research: its Saponin can suppress endameeba histolytica's growth in vitro and in vivo.Anemonin is antibiotic effective ingredient, to inhibitory action such as diphtheria corynebacterium, staphylococcus, streptococcus, escherichia coli, tubercule bacillus.The antibacterial activity of Anemonin also have synergism with strepto-.The Radix Pulsatillae also has antiviral in addition, and calmness, analgesia and spasmolytic are promoted the effect of gastrointestinal motility.
Berberine sulfate: this product only has faint bacteriostasis to antibacterial, but the intestinal infection that dysentery bacterium, escherichia coli are caused is effective.Chinese medicine and berberine sulfate containing tannin share rear has a broad antifungal spectrum, external to multiple Grain-positive and the equal tool bacteriostasis of negative bacterium.Clinical practice: be mainly used in treating the intestinal infections such as gastroenteritis, bacillary dysentery etc.Pharmacological research: berberine sulfate has stronger inhibitory action to golden Portugal bacterium, vibrio cholera, shigella, Bacillus typhi, diphtheria corynebacterium etc., antibacterial during low concentration, during high concentration, sterilizes.Infected by influenza, ameba, leptospira, some dermatophytes also have certain inhibitory action.There is no cross resistance with penicillin, streptomycin etc.
Above-mentioned raw materials medicine compatibility, strengthened the antibacterial ability to pathogenic bacterium such as gold-coloured staphylococci, escherichia coli, Salmonellas, improved the inhibitory action to ameba.Self embodies synergistic function compatibility, and with Multiple Classes of Antibiotics without cross resistance, also show synergism.
Above-mentioned crude drug compatibility has guaranteed its medicinal ingredient, and described poplar flower extract, Radix Sophorae Flavescentis extract, Radix Pulsatillae extract can adopt common extracting method preparation.
Further, the present invention proposes to adopt supercritical CO
2extraction prepares poplar flower extract, Radix Sophorae Flavescentis extract, Radix Pulsatillae extract; What it was concrete is extracted as:
To pulverize, sieve through the flower of poplar of super-dry, use supercritical CO
2extraction preparation: extracting pressure 30Mpa, 45 ℃ of extraction temperature, extraction CO
2flow 40kg/h, once reinforced, the one-level extraction, the second-order separation, extract 1.5 hours and collect extract once, every 30 minutes, collects once later; Extract 3 hours, merging filtrate, filter; 60 ℃ of vacuum dryings, make the poplar flower extract that every 1 gram is equivalent to 20 gram crude drugs;
To pulverize, sieve through the Radix Sophorae Flavescentis of super-dry, by after Radix Sophorae Flavescentis powder and ammonia mixing, soak 12～24 hours, use supercritical CO
2extraction preparation: extracting pressure 35Mpa, 35 ℃ of extraction temperature, extraction CO
2flow 40kg/h, once reinforced, the one-level extraction, the second-order separation, in extraction, the ethanol of minute 3 injection volumetric concentrations 75%, extract 1.5 hours and collect extract once, every 30 minutes, collects once later; Extract 8 hours, merging filtrate, filter; 60 ℃ of vacuum dryings, make the Radix Sophorae Flavescentis extract that every 1 gram is equivalent to 20 gram crude drugs;
To pulverize, sieve through the Radix Pulsatillae of super-dry, use supercritical CO
2extraction preparation: extracting pressure 30Mpa, 45 ℃ of extraction temperature, extraction CO
2flow 40kg/h, once reinforced, the one-level extraction, the second-order separation, extract 1.5 hours and collect extract once, every 30 minutes, collects once later; Extract 3 hours, merging filtrate, filter; 60 ℃ of vacuum dryings, make the Radix Pulsatillae extract that every 1 gram is equivalent to 20 gram crude drugs.
Certainly, above-mentioned concrete parameter can be adjusted as the case may be accordingly, described supercritical CO
2extraction does not affect the application of extract prepared by additive method.
Below further provide its concrete component proportion of effective ingredient, as shown in the table:
| Embodiment 1 | Poplar flower extract 50 grams | Radix Sophorae Flavescentis extract 30 grams | Radix Pulsatillae extract 20 grams | Berberine sulfate 5 grams |
| Embodiment 2 | Poplar flower extract 55 grams | Radix Sophorae Flavescentis extract 30 grams | Radix Pulsatillae extract 20 grams | Berberine sulfate 10 grams |
| Embodiment 3 | Poplar flower extract 60 grams | Radix Sophorae Flavescentis extract 35 grams | Radix Pulsatillae extract 20 grams | Berberine sulfate 10 grams |
| Embodiment 4 | Poplar flower extract 60 grams | Radix Sophorae Flavescentis extract 40 grams | Radix Pulsatillae extract 20 grams | Berberine sulfate 10 grams |
| Embodiment 5 | Poplar flower extract 70 grams | Radix Sophorae Flavescentis extract 50 grams | Radix Pulsatillae extract 20 grams | Berberine sulfate 10 grams |
| Embodiment 6 | Poplar flower extract 75 grams | Radix Sophorae Flavescentis extract 55 grams | Radix Pulsatillae extract 30 grams | Berberine sulfate 10 grams |
| Embodiment 7 | Poplar flower extract 80 grams | Radix Sophorae Flavescentis extract 60 grams | Radix Pulsatillae extract 40 grams | Berberine sulfate 10 grams |
| Embodiment 8 | Poplar flower extract 85 grams | Radix Sophorae Flavescentis extract 65 grams | Radix Pulsatillae extract 50 grams | Berberine sulfate 15 grams |
| Embodiment 9 | Poplar flower extract 90 grams | Radix Sophorae Flavescentis extract 70 grams | Radix Pulsatillae extract 55 grams | Berberine sulfate 15 grams |
| Embodiment | Poplar flower extract 100 grams | Radix Sophorae Flavescentis extract 80 grams | Radix Pulsatillae extract 60 grams | Berberine sulfate 15 grams |
When carrying out the preparation of compound poplar flower oral liquid, also comprise corresponding adjuvant except effective ingredient, be specially:
The sodium benzoate of mass concentration 0.1～2.0%, and volumetric concentration is respectively 0.5～2.5% benzyl alcohol, 3.0～5.0% glycerin methylal, 1.0～3.0% propylene glycol and 1.0～3.0% Tween 80.
The preparation method of the pure Chinese medicine compound poplar flower oral liquid of filling a prescription according to embodiment 1～10 is:
1) organic solvent is heated to 50～70 ℃, in mass fraction, adds respectively the poplar flower extract of 50～100 parts, the Radix Pulsatillae extract of the Radix Sophorae Flavescentis extract of 30～80 parts and 20～60 parts, obtain preliminary effective ingredient solution after fully mixing;
2) in mass fraction, add 5～15 parts of berberine sulfates in preliminary effective ingredient solution, obtain effective ingredient solution after fully mixing;
3) effective ingredient solution is put in emulsion tank, adds antiseptic, stabilizing agent, emulsifying agent, add water to enoughly, fully emulsified 10～30 minutes, obtain compound poplar flower oral liquid.
Concrete, described organic solvent is propylene glycol, and described antiseptic comprises sodium benzoate, benzyl alcohol, and described stabilizing agent is glycerin methylal, and described emulsifying agent is Tween 80;
In the compound poplar flower oral liquid completed in preparation, the volumetric concentration of propylene glycol is 1.0～3.0%, and the mass concentration of sodium benzoate is 0.1～2.0%, and the volumetric concentration of benzyl alcohol is 0.5～2.5%, the volumetric concentration of glycerin methylal is 3.0～5.0%, and the volumetric concentration of Tween 80 is 1.0～3.0%.
Concrete kind, the concentration of antiseptic, stabilizing agent, emulsifying agent can be regulated according to specific needs.
Below prepared pure Chinese medicine compound willow oral liquid is carried out to test of pesticide effectiveness research, to verify its therapeutic effect to piglet diarrhea.
1) be diagnosed as 43 suckling pigs of diarrhoea (natural cases), give pure Chinese medicine compound poplar flower oral liquid of the present invention (gavage, 0.1ml/kg), successive administration 3 days.
Cure: at duration of test, piglet clinical symptom disappearance after medication, the anus drying, without red and swollen, feces recovers normal person, is judged to healing.
Take a turn for the better: after drug treatment, sx↓, feces transfer pasty state to by water sample and are considered as taking a turn for the better.
Dead: as the piglet of duration of test death, through clinical observation, to be the lethal diarrhoea piglet of diarrhoea collapse.
Result in clinical application is as shown in table 1:
The diarrhoea of suckling pig is many by due to coli-infection.Result shows: pure Chinese medicine compound poplar flower oral liquid of the present invention is because having sterilization or bacteriostasis, and the HUANGBAI(sic) dysentery that escherichia coli are caused has definite therapeutic effect.
2) be diagnosed as 39 ablactational baby pig of diarrhoea (natural cases), according to state of an illness situation, be divided at random 2 groups.Matched group award poplar flower oral liquid (gavage, 0.1ml/kg), test group give pure Chinese medicine compound poplar flower oral liquid of the present invention (gavage, 0.1ml/kg), successive administration 3 days.
Curative effect determinate standard: after administration, observe spirit, feces, appetite, body temperature, the respiratory variations of pig every day, record its variation and change procedure, Ineffective Cases is cutd open to inspection, judge the cause of death.
Cure: sick pig diarrhoea in medication 3 days stops, and feces is shaped, and anus is clean, and body temperature, appetite, mental restoration are normal, in 1 week not the recidivist be judged to healing.
Produce effects: the basic termination of sick pig diarrhoea in medication 3 days, feces becomes pasty state, and body temperature is normal, and appetite is better, and in 5 days, feces, spirit, the normal person of appetite are judged to produce effects.
Invalid: sick pig after treating 3 days, spirit, appetite, symptom of diarrhea increase the weight of or symptom variation not remarkable, it is invalid to be judged to.
The therapeutic effect of administration after 3 days is as shown in table 2:
Therapeutic outcome shows, matched group cure rate, obvious effective rate, inefficiency are respectively: 52.4%, 23.8%, 23.8%, and test group is respectively: 55.6%, 33.3%, 11.1%; Total effective rate matched group and test group are respectively 77.2%, 88.9%.This shows that oral liquid of the present invention has definite therapeutic effect to piglet diarrhea, and effect exceeds the poplar flower oral liquid of folk prescription.
Claims (10)
1. a compound poplar flower oral liquid, is characterized in that, in mass fraction, its effective ingredient comprises following component:
The poplar flower extract of 50～100 parts, the Radix Sophorae Flavescentis extract of 30～80 parts, the berberine sulfate of the Radix Pulsatillae extract of 20～60 parts and 5～15 parts;
Wherein, poplar flower extract, Radix Sophorae Flavescentis extract, Radix Pulsatillae extract are respectively to using dry flower of poplar, Radix Sophorae Flavescentis, the Radix Pulsatillae as raw material, adopt supercritical CO
2prepared by extraction.
2. compound poplar flower oral liquid as claimed in claim 1, is characterized in that, in mass fraction, its effective ingredient comprises following component:
The poplar flower extract of 60～80 parts, the Radix Sophorae Flavescentis extract of 40～60 parts, the berberine sulfate of the Radix Pulsatillae extract of 20～30 parts and 5～10 parts.
3. compound poplar flower oral liquid as claimed in claim 1, is characterized in that, in mass fraction, its effective ingredient comprises following component:
The poplar flower extract of 60 parts, the Radix Sophorae Flavescentis extract of 40 parts, the berberine sulfate of the Radix Pulsatillae extract of 20 parts and 10 parts.
4. compound poplar flower oral liquid as claimed in claim 1, is characterized in that, in mass fraction, also comprises following component in compound poplar flower oral liquid:
The sodium benzoate of mass concentration 0.1～2.0%, and volumetric concentration is respectively 0.5～2.5% benzyl alcohol, 3.0～5.0% glycerin methylal, 1.0～3.0% propylene glycol and 1.0～3.0% Tween 80.
5. compound poplar flower oral liquid as claimed in claim 1, is characterized in that, described 1ml compound poplar flower oral liquid is equivalent to the primary crude drug of 1.35～1.5g.
6. compound poplar flower oral liquid as claimed in claim 1, is characterized in that, being prepared as of described poplar flower extract, Radix Sophorae Flavescentis extract, Radix Pulsatillae extract:
To pulverize, sieve through the flower of poplar of super-dry, use supercritical CO
2extraction preparation: extracting pressure 30Mpa, 45 ℃ of extraction temperature, extraction CO
2flow 40kg/h, once reinforced, the one-level extraction, the second-order separation, extract 1.5 hours and collect extract once, every 30 minutes, collects once later; Extract 3 hours, merging filtrate, filter; 60 ℃ of vacuum dryings, make the poplar flower extract that every 1 gram is equivalent to 20 gram crude drugs;
To pulverize, sieve through the Radix Sophorae Flavescentis of super-dry, by after Radix Sophorae Flavescentis powder and ammonia mixing, soak 12～24 hours, use supercritical CO
2extraction preparation: extracting pressure 35Mpa, 35 ℃ of extraction temperature, extraction CO
2flow 40kg/h, once reinforced, the one-level extraction, the second-order separation, in extraction, the ethanol of minute 3 injection volumetric concentrations 75%, extract 1.5 hours and collect extract once, every 30 minutes, collects once later; Extract 8 hours, merging filtrate, filter; 60 ℃ of vacuum dryings, make the Radix Sophorae Flavescentis extract that every 1 gram is equivalent to 20 gram crude drugs;
To pulverize, sieve through the Radix Pulsatillae of super-dry, use supercritical CO
2extraction preparation: extracting pressure 30Mpa, 45 ℃ of extraction temperature, extraction CO
2flow 40kg/h, once reinforced, the one-level extraction, the second-order separation, extract 1.5 hours and collect extract once, every 30 minutes, collects once later; Extract 3 hours, merging filtrate, filter; 60 ℃ of vacuum dryings, make the Radix Pulsatillae extract that every 1 gram is equivalent to 20 gram crude drugs.
7. the preparation method of a compound poplar flower oral liquid, is characterized in that, comprises the following steps:
1) organic solvent is heated to 50～70 ℃, in mass fraction, adds respectively the poplar flower extract of 50～100 parts, the Radix Pulsatillae extract of the Radix Sophorae Flavescentis extract of 30～80 parts and 20～60 parts, obtain preliminary effective ingredient solution after fully mixing;
2) in mass fraction, add 5～15 parts of berberine sulfates in preliminary effective ingredient solution, obtain effective ingredient solution after fully mixing;
3) effective ingredient solution is put in emulsion tank, adds antiseptic, stabilizing agent, emulsifying agent, add water to enoughly, fully emulsified 10～30 minutes, obtain compound poplar flower oral liquid.
8. the preparation method of compound poplar flower oral liquid as claimed in claim 7 is characterized in that:
Described organic solvent is propylene glycol, and described antiseptic comprises sodium benzoate, benzyl alcohol, and described stabilizing agent is glycerin methylal, and described emulsifying agent is Tween 80;
In compound poplar flower oral liquid, the volumetric concentration of propylene glycol is 1.0～3.0%, and the mass concentration of sodium benzoate is 0.1～2.0%, and the volumetric concentration of benzyl alcohol is 0.5～2.5%, the volumetric concentration of glycerin methylal is 3.0～5.0%, and the volumetric concentration of Tween 80 is 1.0～3.0%.
9. the preparation method of compound poplar flower oral liquid as claimed in claim 7, is characterized in that, being prepared as of described poplar flower extract, Radix Sophorae Flavescentis extract, Radix Pulsatillae extract:
To pulverize, sieve through the flower of poplar of super-dry, use supercritical CO
2extraction preparation: extracting pressure 30Mpa, 45 ℃ of extraction temperature, extraction CO
2flow 40kg/h, once reinforced, the one-level extraction, the second-order separation, extract 1.5 hours and collect extract once, every 30 minutes, collects once later; Extract 3 hours, merging filtrate, filter; 60 ℃ of vacuum dryings, make the poplar flower extract that every 1 gram is equivalent to 20 gram crude drugs;
To pulverize, sieve through the Radix Sophorae Flavescentis of super-dry, by after Radix Sophorae Flavescentis powder and ammonia mixing, soak 12～24 hours, use supercritical CO
2extraction preparation: extracting pressure 35Mpa, 35 ℃ of extraction temperature, extraction CO
2flow 40kg/h, once reinforced, the one-level extraction, the second-order separation, in extraction, the ethanol of minute 3 injection volumetric concentrations 75%, extract 1.5 hours and collect extract once, every 30 minutes, collects once later; Extract 8 hours, merging filtrate, filter; 60 ℃ of vacuum dryings, make the Radix Sophorae Flavescentis extract that every 1 gram is equivalent to 20 gram crude drugs;
To pulverize, sieve through the Radix Pulsatillae of super-dry, use supercritical CO
2extraction preparation: extracting pressure 30Mpa, 45 ℃ of extraction temperature, extraction CO
2flow 40kg/h, once reinforced, the one-level extraction, the second-order separation, extract 1.5 hours and collect extract once, every 30 minutes, collects once later; Extract 3 hours, merging filtrate, filter; 60 ℃ of vacuum dryings, make the Radix Pulsatillae extract that every 1 gram is equivalent to 20 gram crude drugs;
Above-mentioned solution is rendered in emulsion tank, adds antiseptic, stabilizing agent, and emulsifying 10-30 minute obtains pure Chinese medicine compound poplar flower oral liquid.
10. the application of compound poplar flower oral liquid claimed in claim 1 in the medicine of the dysentery for preparing anti-poultry, enteritis, diarrhoea.
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| CN107617024A (en) * | 2017-08-08 | 2018-01-23 | 上海朝翔生物技术有限公司 | Natural galenical that a kind of animal is stimulated the secretion of milk and preparation method thereof |
| CN107854527A (en) * | 2017-12-07 | 2018-03-30 | 成都乾坤动物药业股份有限公司 | A kind of pharmaceutical composition for treating grice diarrhoea and its production and use |
| ES2681489A1 (en) * | 2017-03-06 | 2018-09-13 | Vall Garraf, S.L. | FATTENING ADDITIVE IN ANIMAL NUTRITIONAL COMPOSITION (Machine-translation by Google Translate, not legally binding) |
| CN111905019A (en) * | 2020-09-09 | 2020-11-10 | 张世淇 | Traditional Chinese medicine formula for regulating intestinal tracts of cultured animals and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2681489A1 (en) * | 2017-03-06 | 2018-09-13 | Vall Garraf, S.L. | FATTENING ADDITIVE IN ANIMAL NUTRITIONAL COMPOSITION (Machine-translation by Google Translate, not legally binding) |
| CN107617024A (en) * | 2017-08-08 | 2018-01-23 | 上海朝翔生物技术有限公司 | Natural galenical that a kind of animal is stimulated the secretion of milk and preparation method thereof |
| CN107854527A (en) * | 2017-12-07 | 2018-03-30 | 成都乾坤动物药业股份有限公司 | A kind of pharmaceutical composition for treating grice diarrhoea and its production and use |
| CN111905019A (en) * | 2020-09-09 | 2020-11-10 | 张世淇 | Traditional Chinese medicine formula for regulating intestinal tracts of cultured animals and preparation method thereof |
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Application publication date: 20131218 |