CN103340938A - Preparation method for tartary buckwheat standard extract FT83 and high pure quercetin - Google Patents
Preparation method for tartary buckwheat standard extract FT83 and high pure quercetin Download PDFInfo
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- CN103340938A CN103340938A CN2013102460931A CN201310246093A CN103340938A CN 103340938 A CN103340938 A CN 103340938A CN 2013102460931 A CN2013102460931 A CN 2013102460931A CN 201310246093 A CN201310246093 A CN 201310246093A CN 103340938 A CN103340938 A CN 103340938A
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Abstract
The invention relates to a preparation method for a tartary buckwheat standard extract FT83 and high pure quercetin. The tartary buckwheat standard extract FT83 is obtained by using tartary buckwheat bran as a raw material and through extraction, concentration and drying. A yield of the tartary buckwheat standard extract FT83 is higher than 6.0%; a total flavonoid content is larger than 80.0%; and quercetin content is larger than 30.0%. The high pure quercetin is obtained by separating and purifying the standard extract. The content of the high pure quercetin prepared by the standard extract can reach over 95.0%. Products prepared by the method are stable in quality. The preparation process is simple and pollution-free, and is low in cost and suitable for industrialized production.
Description
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Technical field
The invention belongs to the extraction of active ingredients of plants, refining, purification technique field, specifically is the method for preparing Radix Et Rhizoma Fagopyri Tatarici flavone standard extract FT83 and high-purity Quercetin from bran of Radix Et Rhizoma Fagopyri Tatarici.
Background technology
Radix Et Rhizoma Fagopyri Tatarici (F
Agopyrum tataricum(L.) be Polygonaceae buckwheat platymiscium Gaench.), originate in mountain area, China southwest, extensively plant in the west area.Be traditional crops and the staple food crop of high mountain area, China southwest ethnic groups.Wheat bran is the by-product in the Radix Et Rhizoma Fagopyri Tatarici course of processing, is rich in flavones ingredient.Radix Et Rhizoma Fagopyri Tatarici flavone contains rutin 2.0-4.0%, and Quercetin 0.5-1.0%(structure is seen accompanying drawing 1), be the raw material of development functionality health food.
The relevant patent of having authorized " a kind of method of extracting flavone from bran of Radix Et Rhizoma Fagopyri Tatarici " (publication number CN 101062114 A) is made graininess, defat, extraction, concentrated, centrifugal, dry with bran of Radix Et Rhizoma Fagopyri Tatarici.This invention preparation process adopts loaded down with trivial details steps such as granulation, defat, and industrialization acquires a certain degree of difficulty.This method uses the flavones content of spectrophotography Detection and Extraction thing greater than 75%, and error is very big, and fails to carry out the content analysis of main component.
Quercetin has the physiologically actives such as increment of coronary artery dilator, reduction capillary permeability and fragility, blood fat reducing, blood sugar lowering, blood pressure lowering, antiplatelet aggregation, antiinflammatory, antiviral, antiallergic, analgesia, oxidation and removing free radicals, adjusting immunologic function, anti peroxidation of lipid, inhibition tumor cell, is the natural resources of natural medicine and functional health-care food.Quercetin is distributed more widely in plant, and the preparation method of having reported is various.Be raw material with the bran of Radix Et Rhizoma Fagopyri Tatarici, with simple and easy method rutin be degraded to Quercetin, by column chromatographic isolation and purification, prepare the method for high-purity Quercetin, still be not reported.
Summary of the invention
The purpose of this invention is to provide a kind of is raw material with the bran of Radix Et Rhizoma Fagopyri Tatarici, and preparation is rich in the Radix Et Rhizoma Fagopyri Tatarici standard extract FT83 of Quercetin and is prepared the method for highly purified Quercetin.
In order to realize above-mentioned purpose of the present invention, technical scheme of the present invention is as follows:
Be raw material with the bran of Radix Et Rhizoma Fagopyri Tatarici, in low-alcohol solution, the rutin in the bran of Radix Et Rhizoma Fagopyri Tatarici degraded, be converted into Quercetin, the Radix Et Rhizoma Fagopyri Tatarici standard extract FT83 of Quercetin is rich in preparation, is raw material with this standard extract further, through column chromatographic isolation and purification, prepare highly purified Quercetin.Concrete grammar is as follows:
1) bran of Radix Et Rhizoma Fagopyri Tatarici is immersed in the lower alcohol of 60-95%, liquid ratio is 1:6-1:12, and stir once every day, soaks 7-9 days, extracts three times, filters, and merging filtrate obtains alcohol extract; Or to use ultrasonic extraction, extraction time be 30-60min/ time, supersound extraction 3 times, and supersonic frequency is not less than 26 KHZ;
2) the said extracted solution decompression is concentrated to sample solvent and does not contain alcohol substantially, centrifugal 5-10 min, abandoning supernatant, the precipitation part adds the suitable quantity of water suspendible, cross 60 mesh sieves, regulate relative density 1.05-1.08, spray drying, perhaps will precipitate directly freezing doing or heat drying or vacuum drying or drying under reduced pressure of part, namely get Radix Et Rhizoma Fagopyri Tatarici standard extract FT83;
3) step 2) the Radix Et Rhizoma Fagopyri Tatarici standard extract FT83 of gained uses column chromatography purification, with methanol or the alcohol solvent gradient elution of variable concentrations (water, 20%, 40%, 60%, 80%, 90%, 100%); Perhaps with not on year-on-year basis row chloroform and methanol (40:1-20:1) gradient elution.Detect with silica gel thin-layer chromatography (TLC), instruct and collect the Quercetin position, merge enrichment Quercetin position sample solution, through concentrate, drying, dissolve with methanol, recrystallization 2 times, namely get the high-purity Quercetin.
Described bran of Radix Et Rhizoma Fagopyri Tatarici is kind skin discarded in the Radix Et Rhizoma Fagopyri Tatarici seed course of processing, and the main flavone component in the bran of Radix Et Rhizoma Fagopyri Tatarici is rutin and Quercetin, and bran of Radix Et Rhizoma Fagopyri Tatarici contains rutin 2.0-4.0%, Quercetin 0.5-1.0%.Rutin in the bran of Radix Et Rhizoma Fagopyri Tatarici in methanol, ethanol equal solvent natural degradation or in above solvent the method for ultrasound wave assistant degradation rutin is all changed into Quercetin basically.
The supersonic frequency of described ultrasonic extraction is not less than 26 KHZ.
2) the described centrifugal rotational speed of step is 3000-5000r/min.
During column chromatography purification described in the step 3), the parting material of column chromatography is silica gel, or aluminium oxide, or polyamide, when being parting material with silica gel or aluminium oxide, is eluant with the mixed solvent of chloroform and methanol (40:1-20:1); When being parting material with polyamide, be eluant with methanol or the ethanol of different volumes concentration (water, 20%, 40%, 60%, 80%, 100%).
The condition of thin layer chromatography described in the step 3) is developing solvent: benzene-ethyl acetate-formic acid 4:5:1; Developer: 5% ferric chloride alcoholic solution.
Extract the Radix Et Rhizoma Fagopyri Tatarici standard extract FT83 of preparation with method of the present invention from bran of Radix Et Rhizoma Fagopyri Tatarici, to yellow powder, the Semen Fagopyri Esculenti peat-reek is arranged, mildly bitter flavor for faint yellow.Be slightly soluble in hot water, easily be dissolved in ethanol, methanol.Ultraviolet spectrophotometry (UV) is measured, and general flavone content is greater than 80.0%, high performance liquid chromatography (HPLC) quantitative analysis, and quercetin content is not less than 30%, and yield press raw material calculating, is not less than 6.0%.By the high-purity Quercetin of this standard extract preparation, content reaches (HPLC) more than 95.0%.The product that the method that provides by this invention prepares, steady quality, preparation technology is simple, pollution-free, cost is low, is suitable for suitability for industrialized production.
Description of drawings
Fig. 1 is the chemical structural drawing of rutin and Quercetin.
Fig. 2 is the HPLC figure of the Quercetin after the purified crystals.
Fig. 3 is the variation tendency HPLC figure of Quercetin and rutin.
Fig. 4 is the variation tendency TLC figure of Quercetin and rutin.
The specific embodiment
Further specify method of the present invention with embodiment below, but do not limit the present invention with this.
Embodiment 1:
(1) gets bran of Radix Et Rhizoma Fagopyri Tatarici 10 g, with 10 times of weight, 75% ethanol merceration, to soak 9 days at every turn, stir once every day, filter, extract three times, merging filtrate, being concentrated to does not have the alcohol flavor substantially in the sample solution, centrifugal 6 min(3500 commentaries on classics/min), abandoning supernatant, the precipitation part obtains Radix Et Rhizoma Fagopyri Tatarici standard extract FT83 product 0.63 g at 60 ℃ of following drying under reduced pressure.Detect general flavone content 84.1% with ultraviolet spectroscopy (UV), with high performance liquid chromatography (HPLC) quantitative analysis, quercetin content 33.1% does not detect the existence of rutin.Above-mentioned Radix Et Rhizoma Fagopyri Tatarici standard extract FT83, use dmso solution, filter, last polyamide column chromatography separation and purification, ethanol gradient elution with different volumes concentration (water, 20%, 40%, 60%, 80%, 100%), silica gel G thin layer chromatography (TLC) detects, and the TLC condition is: benzene-ethyl acetate-formic acid (4:5:1) is developing solvent; 5% ferric chloride alcoholic solution is developer, collects the Quercetin position, merges enrichment Quercetin position, concentrate, and drying, dissolve with methanol and recrystallization 2 times filter, and drying obtains high-purity Quercetin 131 mg.High performance liquid chromatography (HPLC) detects (Fig. 2), the content 96.9% of Quercetin.Detection method is with embodiment 2.
(2) sample is taken a sample 1 time (about 1ml) the continuous sampling Ninth Heaven every day in (1) in soaking the primary process of extraction, the sample of getting is by the 0.45um microporous filter membrane, and the special-purpose bottle of the liquid chromatograph of packing into is sealed with sealing film, place, to be measured as sample solution.This sample is monitored the changes of contents trend of rutin and Quercetin with high performance liquid chromatography (HPLC) and silica gel G thin layer chromatography (TLC).
Rutin and Quercetin are measured according to high performance liquid chromatography (HPLC) (with 2010 editions one appendix VI D of Chinese Pharmacopoeia method).
Instrument Agilent1200 type high performance liquid chromatograph; Chromatographic column ZORBAX XDB-C
18(150 * 4.6 mm, 5 um), 30 ℃ of column temperatures; With acetonitrile: phosphate buffer (0.34% phosphoric acid solution) (4: 96) beginning gradient elution, mobile phase becomes acetonitrile in 0 → 40 min: phosphate buffer (0.34% phosphoric acid solution) (40:60), flow velocity is per minute 1 ml; Detecting wavelength is 354 nm.
Rutin and each 7.5mg of Quercetin sample that is dried to constant weight got in the preparation of reference substance solution respectively, places the 25ml volumetric flask, and the dissolve with methanol with 70% is settled to graduation mark, shakes up, with the filtering with microporous membrane of 0.45um, namely.
Detect accurate sample solution and each 5-10uL of reference substance solution of drawing, inject chromatograph of liquid, measure, namely.
Condition benzene-ethyl acetate-formic acid (4:5:1) that TLC detects is developing solvent, and 5% ferric chloride alcoholic solution is developer, and sample is that above-mentioned HPLC detects specimen in use.
The result:
The HPLC analysis result is as shown in Figure 3: the changes of contents of rutin and Quercetin and the time of immersion are proportionate.
TLC testing result such as Fig. 4 can observe the changes of contents trend of rutin and Quercetin.Rutin reduces gradually, and Quercetin increases gradually.
Embodiment 2:
(1) gets bran of Radix Et Rhizoma Fagopyri Tatarici 1 kg, with 6 times of weight, 70% ethanol merceration, to soak 8 days at every turn, stir once every day, filter, extract three times, merging filtrate, being concentrated to does not have the alcohol flavor substantially in the sample solution, centrifugal 5 min(5000 commentaries on classics/min), abandoning supernatant, the precipitation part obtains Radix Et Rhizoma Fagopyri Tatarici standard extract FT83 product 66.5 g 60 ℃ of following forced air dryings.Detect general flavone content 83.5% with ultraviolet spectroscopy (UV), with high performance liquid chromatography (HPLC) quantitative analysis, quercetin content 32.3% does not detect the existence of rutin.Get the above-mentioned Radix Et Rhizoma Fagopyri Tatarici standard extract of 4.0g FT83, dmso solution, filter, last polyamide column chromatography separation and purification, methanol gradient elution with different volumes concentration (water, 20%, 40%, 60%, 80%, 100%), silica gel G thin layer chromatography (TLC) detects, and the TLC condition is: benzene-ethyl acetate-formic acid (4:5:1) is developing solvent; 5% ferric chloride alcoholic solution is developer, collects the Quercetin position, merges enrichment Quercetin position, concentrates, drying, dissolve with methanol and recrystallization 2 times filter, drying obtains high-purity Quercetin 1.1 g. high performance liquid chromatography (HPLC) and detects (Fig. 4), the content 95.7% of Quercetin.
(2) assay
Total flavones is measured at 420 nm wavelength places according to spectrophotography (appendix VA of Chinese Pharmacopoeia version in 2010).
Reagent: aluminum chloride (AR), methanol (AR), dimethyl sulfoxide (AR), pure water.
The reagent preparation:
1% aluminum trichloride solution: take by weighing the 1.0g aluminum chloride and add the suitable quantity of water dissolving, fixed molten to 100ml, shake up.70% methanol solution: draw 70ml methanol, add water to 100 ml, shake up namely.
The preparation of sample solution: get the Radix Et Rhizoma Fagopyri Tatarici flavone standard extract 40.0mg that is rich in Quercetin, place the volumetric flask of 200 ml, add the dimethyl sulfoxide of 1ml, dissolve, add 70% methanol solution again, be settled to graduation mark, shake up, standby.
The preparation of reference substance: precision takes by weighing the reference substance rutin 12.5mg that is dried to constant weight, places the volumetric flask of 50 ml, and the dissolve with methanol with 70% is settled to graduation mark, shakes up, and is standby.
The preparation of standard curve: get above-mentioned reference substance solution 200,400,500 respectively, 600,800,1000ul is in the teat glass of 20ml, adding 800,600,500 respectively, 70% the methanol of 400,200,0ul is supplied 1ml, add 1% aluminum trichloride solution 7ml more respectively, shake up, under 420nm, measure its absorbance, sampling amount with rutin is abscissa (X), and light absorption value is that vertical coordinate (Y) is done regression curve.
The mensuration of sample: get above-mentioned sample solution 1ml in the teat glass of 20ml, add 1% aluminum trichloride solution 7ml, shake up, under 420nm, measure its absorbance, bring its absorbance into regression curve and calculate content of total flavone in its sample (in rutin).
Rutin and Quercetin are measured according to high performance liquid chromatography (HPLC) (with 2010 editions one appendix VI D of Chinese Pharmacopoeia method).
Instrument Agilent1200 type high performance liquid chromatograph; Chromatographic column ZORBAX XDB-C
18(150 * 4.6 mm, 5 um), 30 ℃ of column temperatures; With acetonitrile: phosphate buffer (0.34% phosphoric acid solution) (4: 96) beginning gradient elution, mobile phase becomes acetonitrile in 0 → 40 min: phosphate buffer (0.34% phosphoric acid solution) (40:60), flow velocity is per minute 1 ml; Detecting wavelength is 354 nm.
Rutin and each 7.5mg of Quercetin sample that is dried to constant weight got in the preparation of reference substance solution respectively, places 25ml capacity product, and the dissolve with methanol with 70% is settled to graduation mark, shakes up, with the filtering with microporous membrane of 0.45um, namely.
The standard curve preparation is the accurate reference substance solution 1.0,2.0,4.0 of drawing respectively, 8.0,10.0,14.0,20.0 μ L measures peak area by above-mentioned chromatographic condition, is abscissa with the amount (mg) of reference substance, peak area (A) is vertical coordinate drawing standard curve.
The preparation precision of sample solution take by weighing be rich in Quercetin Radix Et Rhizoma Fagopyri Tatarici flavone standard extract 20 mg in the volumetric flask of 25ml, the dimethyl sulfoxide that adds 0.5 ml makes its dissolving, add 70% methanol solution again and be settled to graduation mark and shake up, with the filtering with microporous membrane of 0.45 um, namely get standby.
Measure accurate need testing solution 5-10 uL that draw, inject chromatograph of liquid, measure, namely.Content with standard curve meter rutin and Quercetin.
Embodiment 3:
Get bran of Radix Et Rhizoma Fagopyri Tatarici 10 kg, with 11 times of weight, 80% ethanol merceration, stir once every day, the each immersion 9 days extracted three times, filters, merging filtrate, be concentrated to small size, centrifugal 8 min(4000 commentaries on classics/min), abandon supernatant, the precipitation part adds water and stirs suspendible, regulating relative density is 1.06, filters with 60 mesh sieves, and spray drying obtains Radix Et Rhizoma Fagopyri Tatarici standard extract FT83 product 650g.Detect general flavone content 85.4% with ultraviolet spectroscopy (UV), with high performance liquid chromatography (HPLC) quantitative analysis, quercetin content 31.5% does not detect the existence of rutin.Get the above-mentioned Radix Et Rhizoma Fagopyri Tatarici standard extract of 10 g FT83, with methanol and dmso solution, use silica gel mixed sample, last silica gel column chromatography separating purification, with chloroform: two gradient elutions of methanol (40:1,30:1), the Fractional Collections eluent, silica gel G thin layer chromatography (TLC) detects, and the TLC condition is: benzene-ethyl acetate-formic acid (4:5:1) is developing solvent.5% ferric chloride alcoholic solution is developer, collects Quercetin position sample solution, merges enrichment Quercetin position, concentrate, and drying, dissolve with methanol and recrystallization 2 times filter, and drying obtains high-purity Quercetin 2.5 g.High performance liquid chromatography (HPLC) detects, the content 96.3% of Quercetin.Detection method is with embodiment 2.
Embodiment 4:
Get bran of Radix Et Rhizoma Fagopyri Tatarici 5.0 kg, with 12 times of amount 70% methanol supersound extraction, each 45min, supersound extraction three times, filter, merging filtrate concentrates, be concentrated to small size, centrifugal 7 min(3000 commentaries on classics/min), abandon supernatant will precipitate part and add low amounts of water stirring suspendible, lyophilization obtains Radix Et Rhizoma Fagopyri Tatarici standard extract FT83 product 335.2g.Detect general flavone content 83.7% with ultraviolet spectroscopy (UV), with high performance liquid chromatography (HPLC) quantitative analysis, quercetin content 33.7% does not detect the existence of rutin.Get the above-mentioned Radix Et Rhizoma Fagopyri Tatarici standard extract of 6.0 g FT83, dmso solution, last alumina column chromatography separation and purification, with chloroform: three gradient elutions of methanol (40:1,30:1,20:1), the Fractional Collections eluent, silica gel G thin layer chromatography (TLC) detects, and the TLC condition is: benzene-ethyl acetate-formic acid (4:5:1) is developing solvent; 5% ferric chloride alcoholic solution is developer.Collect Quercetin position sample solution, merge enrichment Quercetin position, concentrate, drying, dissolve with methanol and recrystallization 2 times filter, and drying obtains high-purity Quercetin 1.4 g. high performance liquid chromatography (HPLC) and detects the content 97.5% of Quercetin.Detection method is with embodiment 2.
Claims (6)
1. the preparation method of a Radix Et Rhizoma Fagopyri Tatarici standard extract FT83 and high-purity Quercetin is characterized in that, is raw material with the bran of Radix Et Rhizoma Fagopyri Tatarici, carries out according to the following steps:
1) bran of Radix Et Rhizoma Fagopyri Tatarici is immersed in the lower alcohol of 60-95%, liquid ratio is 1:6-1:12, and stir once every day, soaks 7-9 days, extracts three times, filters, and merging filtrate obtains alcohol extract; Or to use ultrasonic extraction, extraction time be 30-60min/ time, supersound extraction 3 times;
2) the said extracted solution decompression is concentrated to sample solvent and does not contain alcohol substantially, centrifugal 5-10 min, abandoning supernatant, the precipitation part adds the suitable quantity of water suspendible, cross 60 mesh sieves, regulate relative density 1.05-1.08, spray drying, perhaps will precipitate part directly lyophilization or heat drying or vacuum drying or drying under reduced pressure, namely get Radix Et Rhizoma Fagopyri Tatarici standard extract FT83;
3) step 2) the Radix Et Rhizoma Fagopyri Tatarici standard extract FT83 of gained uses column chromatography purification, and with different volumes concentration: 20%, 40%, 60%, 80%, 90%, 100% methanol or ethanol or water are as solvent gradient elution; Perhaps with not on year-on-year basis row chloroform and methanol 40:1-20:1 gradient elution; Detect with silica gel thin-layer chromatography, instruct and collect the Quercetin position, merge enrichment Quercetin position, through concentrate, drying, dissolve with methanol, recrystallization 2 times, namely get the high-purity Quercetin.
2. the preparation method of Radix Et Rhizoma Fagopyri Tatarici standard extract FT83 according to claim 1 and Quercetin is characterized in that described bran of Radix Et Rhizoma Fagopyri Tatarici is kind skin discarded in the Radix Et Rhizoma Fagopyri Tatarici seed course of processing, and bran of Radix Et Rhizoma Fagopyri Tatarici contains rutin 2.0-4.0%, Quercetin 0.5-1.0%.
3. the preparation method of Radix Et Rhizoma Fagopyri Tatarici standard extract FT83 according to claim 1 and Quercetin is characterized in that, the supersonic frequency of ultrasonic extraction is not less than 26 KHZ.
4. the preparation method of Radix Et Rhizoma Fagopyri Tatarici standard extract FT83 according to claim 1 and Quercetin is characterized in that 2) the described centrifugal rotational speed of step is 3000-5000r/min.
5. the preparation method of Radix Et Rhizoma Fagopyri Tatarici standard extract FT83 according to claim 1 and Quercetin, when it is characterized in that in the step 3) column chromatography purification, the parting material of column chromatography is silica gel, or aluminium oxide, or polyamide, when being parting material with silica gel or aluminium oxide, be eluant with the mixed solvent of chloroform and methanol 40:1-20:1; When being parting material with polyamide, use different volumes concentration: 20%, 40%, 60%, 80%, 100% methanol or ethanol, perhaps water is eluant.
6. the preparation method of Radix Et Rhizoma Fagopyri Tatarici standard extract FT83 according to claim 1 and Quercetin, it is characterized in that the condition of thin layer chromatography in the step 3) is developing solvent: benzene-ethyl acetate-formic acid is 4-5-1; Developer: 5% ferric chloride alcoholic solution.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104459012A (en) * | 2014-12-26 | 2015-03-25 | 吉林化工学院 | Thin-layer identification method of effective part of flavones of fruit of rosa davurica pall |
| CN105582062A (en) * | 2016-01-15 | 2016-05-18 | 中国农业科学院作物科学研究所 | Tartary buckwheat bran extract and externally-used preparation used for skin and containing extract |
| CN105920114A (en) * | 2016-04-18 | 2016-09-07 | 浙江大学 | Black tartary buckwheat rice extract with blood sugar decreasing function and preparation method thereof |
| CN109369733A (en) * | 2018-10-30 | 2019-02-22 | 湖南华诚生物资源股份有限公司 | A method of extracting a variety of flavone compounds simultaneously from leaf of Radix Et Rhizoma Fagopyri Tatarici |
| CN112305103A (en) * | 2020-10-21 | 2021-02-02 | 北京农学院 | Method for extracting and determining lactucin and lactucin |
| CN115974826A (en) * | 2022-12-28 | 2023-04-18 | 西昌航飞苦荞科技发展有限公司 | Method for preparing tartary buckwheat quercetin from tartary buckwheat flavone extract |
| CN116831262A (en) * | 2023-02-24 | 2023-10-03 | 西昌学院 | Method for producing solid beverage containing bioflavonoids-rutin |
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2013
- 2013-06-20 CN CN2013102460931A patent/CN103340938A/en active Pending
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104459012A (en) * | 2014-12-26 | 2015-03-25 | 吉林化工学院 | Thin-layer identification method of effective part of flavones of fruit of rosa davurica pall |
| CN105582062A (en) * | 2016-01-15 | 2016-05-18 | 中国农业科学院作物科学研究所 | Tartary buckwheat bran extract and externally-used preparation used for skin and containing extract |
| CN105920114A (en) * | 2016-04-18 | 2016-09-07 | 浙江大学 | Black tartary buckwheat rice extract with blood sugar decreasing function and preparation method thereof |
| CN109369733A (en) * | 2018-10-30 | 2019-02-22 | 湖南华诚生物资源股份有限公司 | A method of extracting a variety of flavone compounds simultaneously from leaf of Radix Et Rhizoma Fagopyri Tatarici |
| CN109369733B (en) * | 2018-10-30 | 2020-07-07 | 湖南华诚生物资源股份有限公司 | Method for simultaneously extracting multiple flavonoid compounds from tartary buckwheat leaves |
| CN112305103A (en) * | 2020-10-21 | 2021-02-02 | 北京农学院 | Method for extracting and determining lactucin and lactucin |
| CN115974826A (en) * | 2022-12-28 | 2023-04-18 | 西昌航飞苦荞科技发展有限公司 | Method for preparing tartary buckwheat quercetin from tartary buckwheat flavone extract |
| CN116831262A (en) * | 2023-02-24 | 2023-10-03 | 西昌学院 | Method for producing solid beverage containing bioflavonoids-rutin |
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Application publication date: 20131009 |