CN103196731A - Multiple stain reagent and detection method for identifying breast myoepithelial lesion - Google Patents
Multiple stain reagent and detection method for identifying breast myoepithelial lesion Download PDFInfo
- Publication number
- CN103196731A CN103196731A CN2013101352484A CN201310135248A CN103196731A CN 103196731 A CN103196731 A CN 103196731A CN 2013101352484 A CN2013101352484 A CN 2013101352484A CN 201310135248 A CN201310135248 A CN 201310135248A CN 103196731 A CN103196731 A CN 103196731A
- Authority
- CN
- China
- Prior art keywords
- section
- minutes
- distilled water
- human monoclonal
- pathology
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a multiple stain reagent and a detection method for identifying a breast myoepithelial lesion. The multiple stain reagent comprises 20% of mouse anti-human CK8/18 immunohistochemical monoclonal antibody, 20% of ready-to-use calmodulin mouse anti-human monoclonal antibody, 20% of ready-to-use cytokeratin mouse anti-human monoclonal antibody, 20% of ready-to-use P63 protein mouse anti-human monoclonal antibody, and 20% of actin HHF-35 anti-human monoclonal antibody. When a breast myoepithelial cell is marked, the myoepithelial cell has strong specificity and sensibility, and different target antigens in the same target cell can be identified, so that a cell nucleus, a cell membrane and a cytoplasm can be stained synchronously, the reactivity and the sensibility of the myoepithelial cell are markedly improved, basic structures of a breast acinus and a catheter can be indicated more objectively, and a good assistance effect is exerted on the diagnosis of the breast lesion.
Description
Technical field
The present invention relates to a kind of SABC reagent, especially a kind of for multiple staining reagent and the detection method thereof of differentiating mammary gland myoepithelium pathology.
Background technology
Breast tissue often contains multiple antigenic component, is expressed in cell membrane, tenuigenin and the nucleus different parts of variety classes cell.The mammary gland disease pathological diagnosis will show the not synantigen of myoepithelium, galandular epithelium tissue, often need with multiple antibody dye marker in many sections respectively, if the myoepithelium continued presence does not then have infiltrating carcinoma, if the myoepithelium negative reaction then is mammary gland infiltration cancer (except the blunt duct adenosis).Single antibody of planting shows that DCIS musculoepithelia cell born of the same parents are the jumping characteristic positive, and myoepithelium is discontinuous.If in a section, show multiple tissue antigen simultaneously, often need adopt repeatedly mark mark repeatedly a section, 5 kinds of antibody will dye for 5 times, method is loaded down with trivial details, dyeing time is long, and repeatable operation causes that easily tissue comes off in a section, also wastes time and energy from the methodology angle.
Summary of the invention
Provide a kind of for multiple staining reagent and the detection method thereof of differentiating mammary gland myoepithelium pathology at above the deficiencies in the prior art during technical assignment of the present invention.
The technical solution adopted for the present invention to solve the technical problems is: a kind of for multiple staining reagent and the detection method thereof of differentiating mammary gland myoepithelium pathology, this reagent is made up of the antibody of following weight portion: mouse-anti people CK8/18 SABC monoclonal antibody 20%, instant calmodulin mouse-anti human monoclonal antibodies 20%, instant cytokeratin mouse-anti human monoclonal antibodies 20%, instant P63 albumen mouse-anti human monoclonal antibodies 20%, actin HHF-35 mouse-anti human monoclonal antibodies 20%, its method for making is that equivalent is drawn above-mentioned each component antibody respectively, mix then, get final product at vortex mixer rotation mixing or suction pipe piping and druming mixing, and it is standby to put in 4 ℃ of refrigerators preservation.
Be used for differentiating the detection method of mammary gland myoepithelium pathology, may further comprise the steps:
Step 1, the mammary gland myoepithelium tissue of gathering through dehydration, behind the transparent and waxdip, after carrying out paraffin embedding, cut into slices, thickness is 3-5um, the tissue that is suspected to have pathology is elected to be immune labeled, is under 58 ℃-60 ℃ in temperature, be put into roasting the section 2-3 hour in the constant temperature oven, after paraffin process dewaxing in the section, the aquation, with TBS liquid flushing section 3 times, each 3 minutes;
Step 2, will cut into slices through endogenous peroxydase blocking-up, be that 3% freshly prepared hydrogen peroxide is hatched section 5 minutes with concentration, cuts into slices 2 times each 3 minutes then with distilled water flushing;
Step 3, repair liquid with EDTA antigen retrieval is carried out in section;
Step 4, tangential section add non-immune serum, hatch section 10 minutes under the room temperature;
Step 5, remove the non-immune serum in the section, tangential section is added and is redyed color reagent, hatches section 30-60 minute under 17 ℃ of-33 ℃ of temperature;
Step 6, add and make MACH 2 Double Stain ELIAS secondary antibody reagent, incubated at room section 30 minutes with TBS liquid flushing section 3 times, each 3 minutes, is used distilled water flushing 2 times, each 3 minutes again;
Step 7, remove the distilled water in the section after, added AP-red developer incubated at room section 10-20 minute, with distilled water flushing 2 times, each 3 minutes, examine under a microscope, grasp dyeing time, positive signal is red, after observation is finished, with TBS liquid flushing section 3 times, each 3 minutes, use distilled water flushing again 2 times, each 3 minutes, adopting the pH value then is 2.0, concentration was 0.05% HCl incubated at room section 30 minutes, with TBS liquid flushing section 2 times, each 3 minutes;
Step 8, add DAB developer incubated at room section after 5 minutes, with distilled water flushing section 2 times, each 3 minutes, examine under a microscope, grasp dyeing time, positive signal is yellow or pale brown look, after observation finishes, with distilled water flushing section 3 times, each 3 minutes;
Step 9, remove the distilled water in the section, add haematine understain 30-60 after second, with distilled water flushing 2 times, each 3 minutes;
Step 10, remove the distilled water in the section, when section is not dried, directly drip water-based mountant mounting, 60 ℃ of oven dry 15-30 minute after the water-based mountant drying, are treated the nature cooling, get final product with the neutral gum mounting again.
In melting point of paraffin wax described in the above-mentioned steps one less than 60 ℃.
Repair liquid for to boil 20 minutes at EDTA described in the above-mentioned steps three under 100 ℃ of temperature, its pH is 8.0.
Be matching while using at AP-red developer described in the above-mentioned steps seven.
Multiple staining reagent of the present invention has stronger specificity and susceptibility when mark mammary gland musculoepithelia cell.Can identify target antigens different in the same target cell respectively, make nucleus, cell membrane and tenuigenin painted synchronously, reactivity and the susceptibility of musculoepithelia cell have obviously been increased, more can demonstrate the basic structure of mammary gland acinus and conduit objectively, good booster action is played in the diagnosis of breast lesion.
Observe by experiment, we find that multiple staining reagent of the present invention is used for the immunohistochemistry mark and has the effect that antibody is had complementary advantages, two kinds of antibody capables react with isocellular different target antigens respectively, the common identification target cell, point out this technology that the collaborative potentiality that detect the tumor tissues source are arranged, especially help pathologic diagnosis of tumor.
This method only is applicable to antigen at present not in the double-tagging of same position, and still not being suitable for two antigens may be in same positioned detection.
Generating technique effect of the present invention is as follows:
1, can sync mark two kinds of target cells of different tissues, again can the identical target cell of mark in different target antigens;
2, avoided single mark discontinuity, jumping characteristic, this labelling method makes the myoepithelium continued labelling, is easy to detect and confirms the special mess microinvasive carcinoma, has improved repeatability and the accuracy rate of diagnosis;
3, especially in the aspiration biopsy small sample, once individual multiple dyeing of cutting into slices can reduce quite a few and fail to pinpoint a disease in diagnosis or because of the unnecessary repeated puncture biopsy of the uncertain patient of causing of diagnostic result;
4, uncontinuity and the jumping characteristic of single labelled reagent dyeing have been overcome, when making single mark musculoepithelia cell seem to be lack as or to be shown as the myoepithelium structure continuous in the structural break zone, also obviously more single mark is remarkable for its response intensity, being different from infiltration ductal carcinomas of breast does not have positive reaction, makes myoepithelium and galandular epithelium distribute and comes into plain view.
5, individual section, simultaneously different colours, improved the sharpness of color contrast;
6, new mounting method wet envelope method different from the past can be protected look for a long time, and is colour-fast;
7, easy, economic, practical, both be applicable to subject study experiment, be applicable to the clinical pathology diagnosis again, seek the tumor tissues source and have very big potentiality uniting.
Embodiment
A kind of for the multiple staining SABC reagent of differentiating mammary gland myoepithelium pathology, this SABC reagent is made up of the antibody of following weight portion: mouse-anti people CK8/18 SABC monoclonal antibody 20%, instant calmodulin mouse-anti human monoclonal antibodies 20%, instant cytokeratin mouse-anti human monoclonal antibodies 20%, instant P63 albumen mouse-anti human monoclonal antibodies 20%, actin HHF-35 mouse-anti human monoclonal antibodies 20%, its method for making is that equivalent is drawn above-mentioned each component antibody respectively, mix then, get final product at vortex mixer rotation mixing or suction pipe piping and druming mixing, and it is standby to put in 4 ℃ of refrigerators preservation.
Did the preliminary experiment of independent dyeing before carrying out multiple staining at first respectively, the condition of its experiment condition during with multiple staining is identical.
This invention multiple staining reagent immunohistochemical staining adopts the horseradish peroxidase HPR detection system with the polymkeric substance link, and concrete grammar is:
Step 1, the mammary gland myoepithelium tissue of gathering through dehydration, behind the transparent and waxdip, after carrying out low-melting paraffin (fusing point＜60 ° C) embedding, cut into slices, thickness is 3-5um, the tissue that is suspected to have pathology is elected to be immune labeled, is under 58 ℃-60 ℃ in temperature, be put into roasting the section 2-3 hour in the constant temperature oven, after paraffin process dewaxing in the section, the aquation, with TBS liquid flushing section 3 times, each 3 minutes;
Step 2, will cut into slices through endogenous peroxydase blocking-up, be that 3% freshly prepared hydrogen peroxide is hatched section 5 minutes with concentration, cuts into slices 2 times each 3 minutes then with distilled water flushing;
Step 3, repair liquid with EDTA antigen retrieval is carried out in section, its EDTA repairs liquid for to boil 20 minutes under 100 ℃ of temperature, and its pH is 8.0;
Step 4, tangential section add non-immune serum, hatch section 10 minutes under the room temperature;
Step 5, remove the non-immune serum in the section, tangential section is added and is redyed color reagent, under 17 ℃ of-33 ℃ of temperature, hatch section 30-60 minute or be placed on overnight incubation in 4 ℃ of refrigerators, carry out in the environment that humidity sealing is closed, purpose is to avoid that the antibody water evaporates causes concentrating of antibody or dry in the multiple staining reagent;
Step 6, add and make MACH 2 Double Stain ELIAS secondary antibody reagent, its MACH 2 Double Stain select for use U.S. Biocare Medical company to produce, incubated at room section 30 minutes, with TBS liquid flushing section 3 times, each 3 minutes, use distilled water flushing again 2 times, each 3 minutes;
Step 7, after removing the distilled water in the section, add AP-red developer incubated at room section 10-20 minute, with distilled water flushing 2 times, each 3 minutes, examine under a microscope, grasp dyeing time according to the development of redness, positive signal is red, after observation is finished, with TBS liquid flushing section 3 times, each 3 minutes, use distilled water flushing again 2 times, each 3 minutes, adopting the pH value then was 2.0, and concentration is 0.05% HCl incubated at room section 30 minutes, purpose is the activity of all antibody in the wash-out deactivation multiple staining reagent, avoid the cross reaction that causes in the double staining, with TBS liquid flushing section 2 times, each 3 minutes;
Step 8, add DAB developer incubated at room section after 5 minutes, with distilled water flushing section 2 times, each 3 minutes, examine under a microscope, grasp dyeing time according to the development of color, positive signal is yellow or pale brown look, after observation finishes, with distilled water flushing section 3 times, each 3 minutes;
Step 9, remove the distilled water in the section, add haematine understain 30-60 after second, with distilled water flushing 2 times, each 3 minutes;
Step 10, remove the distilled water in the section, when section is not dried, directly drip water-based mountant mounting, 60 ℃ of oven dry 15-30 minute after the water-based mountant drying, are treated the nature cooling, get final product with the neutral gum mounting again.
Claims (5)
1. be used for differentiating the multiple staining reagent of mammary gland myoepithelium pathology, it is characterized in that this multiple staining reagent is made up of the antibody of following weight portion: mouse-anti people CK8/18 SABC monoclonal antibody 20%, instant calmodulin mouse-anti human monoclonal antibodies 20%, instant cytokeratin mouse-anti human monoclonal antibodies 20%, instant P63 albumen mouse-anti human monoclonal antibodies 20%, actin HHF-35 mouse-anti human monoclonal antibodies 20%, its method for making is that equivalent is drawn above-mentioned each component antibody respectively, mix then, get final product at vortex mixer rotation mixing or suction pipe piping and druming mixing, and it is standby to put in 4 ℃ of refrigerators preservation.
2. be used for differentiating the detection method of mammary gland myoepithelium pathology, it is characterized in that may further comprise the steps: step 1, the mammary gland myoepithelium tissue of gathering through dehydration, behind the transparent and waxdip, after carrying out paraffin embedding, cut into slices, thickness is 3-5um, be elected to be immune labeled to the tissue that is suspected to have pathology, be under 58 ℃-60 ℃ in temperature, be put in the constant temperature oven roasting section 2-3 hour, the paraffin in the section through dewaxing, aquation after, with TBS liquid flushing section 3 times, each 3 minutes; Step 2, will cut into slices through endogenous peroxydase blocking-up, be that 3% freshly prepared hydrogen peroxide is hatched section 5 minutes with concentration, cuts into slices 2 times each 3 minutes then with distilled water flushing; Step 3, repair liquid with EDTA antigen retrieval is carried out in section; Step 4, tangential section add non-immune serum, hatch section 10 minutes under the room temperature; Step 5, remove the non-immune serum in the section, tangential section is added and is redyed color reagent, hatches section 30-60 minute under 17 ℃ of-33 ℃ of temperature; Step 6, add and make MACH 2 Double Stain ELIAS secondary antibody reagent, incubated at room section 30 minutes with TBS liquid flushing section 3 times, each 3 minutes, is used distilled water flushing 2 times, each 3 minutes again; Step 7, remove the distilled water in the section after, added AP-red developer incubated at room section 10-20 minute, with distilled water flushing 2 times, each 3 minutes, examine under a microscope, grasp dyeing time, positive signal is red, after observation is finished, with TBS liquid flushing section 3 times, each 3 minutes, use distilled water flushing again 2 times, each 3 minutes, adopting the pH value then is 2.0, concentration was 0.05% HCl incubated at room section 30 minutes, with TBS liquid flushing section 2 times, each 3 minutes; Step 8, add DAB developer incubated at room section after 5 minutes, with distilled water flushing section 2 times, each 3 minutes, examine under a microscope, grasp dyeing time, positive signal is yellow or pale brown look, after observation finishes, with distilled water flushing section 3 times, each 3 minutes; Step 9, remove the distilled water in the section, add haematine understain 30-60 after second, with distilled water flushing 2 times, each 3 minutes; Step 10, remove the distilled water in the section, when section is not dried, directly drip water-based mountant mounting, 60 ℃ of oven dry 15-30 minute after the water-based mountant drying, are treated the nature cooling, get final product with the neutral gum mounting again.
3. according to claim 2 for the detection method of differentiating mammary gland myoepithelium pathology, it is characterized in that in melting point of paraffin wax described in the step 1 less than 60 ℃.
4. according to claim 2 for the detection method of differentiating mammary gland myoepithelium pathology, it is characterized in that repairing liquid under 100 ℃ of temperature, to boil 20 minutes at EDTA described in the step 3, its pH is 8.0.
5. according to claim 2 for the detection method of differentiating mammary gland myoepithelium pathology, it is characterized in that at the developer of AP-red described in the step 7 be matching while using.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101352484A CN103196731A (en) | 2013-04-18 | 2013-04-18 | Multiple stain reagent and detection method for identifying breast myoepithelial lesion |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101352484A CN103196731A (en) | 2013-04-18 | 2013-04-18 | Multiple stain reagent and detection method for identifying breast myoepithelial lesion |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103196731A true CN103196731A (en) | 2013-07-10 |
Family
ID=48719424
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013101352484A Pending CN103196731A (en) | 2013-04-18 | 2013-04-18 | Multiple stain reagent and detection method for identifying breast myoepithelial lesion |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103196731A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104447994A (en) * | 2014-12-05 | 2015-03-25 | 福州迈新生物技术开发有限公司 | Tumor suppressor gene p63 monoclonal antibody and application thereof |
| CN108918493A (en) * | 2018-07-24 | 2018-11-30 | 中国科学院深圳先进技术研究院 | A kind of pH fluorescent dye, pH fluorescence probe, preparation method and application |
| CN110850094A (en) * | 2019-11-22 | 2020-02-28 | 西安交通大学 | Immunohistochemical double-label single-staining kit and use method and application thereof |
| CN112964877A (en) * | 2021-03-09 | 2021-06-15 | 河南赛诺特生物技术有限公司 | Immunohistochemical multiple staining kit and staining procedure for identifying mantle cell lymphoma |
| CN113295871A (en) * | 2021-07-02 | 2021-08-24 | 河南赛诺特生物技术有限公司 | Cocktail immunohistochemical kit for diagnosing breast cancer |
| CN113762379A (en) * | 2021-09-07 | 2021-12-07 | 福州迈新生物技术开发有限公司 | Method for generating training data based on immunohistochemistry and storage device |
| CN116086919A (en) * | 2023-02-20 | 2023-05-09 | 深圳裕策生物科技有限公司 | Staining method and kit for lung cancer and/or pancreatic cancer samples |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87101075A (en) * | 1987-06-29 | 1988-04-20 | 蒋俊辉 | Whole dyeing technique of one step of plant tissue polychrome and special combination stain thereof |
| CN1360059A (en) * | 2000-12-18 | 2002-07-24 | 拜尔公司 | Method for specific detection of tumor cell and its precursor in cervix smear by simultaneously measuring at least two different molecular labels |
| CN101329230A (en) * | 2008-07-14 | 2008-12-24 | 中国人民解放军第三军医大学 | Improved method for dyeing immunofluorescence cell |
| CN101395182A (en) * | 2006-03-06 | 2009-03-25 | 西福根有限公司 | Recombinant antibodies for treatment of respiratory syncytial virus infections |
| CN101545911A (en) * | 2008-03-25 | 2009-09-30 | 北京科美东雅生物技术有限公司 | Chemoluminescence immunoassay quantitative measuring kit of cytokeratin 18 and preparation method thereof |
| JP2010148447A (en) * | 2008-12-25 | 2010-07-08 | Canon Inc | Composition for staining retinal tissue and method for staining retinal tissue |
| US20100322502A1 (en) * | 2009-06-18 | 2010-12-23 | Olympus Corporation | Medical diagnosis support device, image processing method, image processing program, and virtual microscope system |
| CN102405238A (en) * | 2009-04-20 | 2012-04-04 | 国立大学法人富山大学 | Antibody cocktail for determining cancer histological type, determination kit and determination method |
| CN102924594A (en) * | 2012-08-03 | 2013-02-13 | 无锡傲锐东源生物科技有限公司 | Anti-tumor-protein-P63 monoclonal antibody and purpose thereof |
-
2013
- 2013-04-18 CN CN2013101352484A patent/CN103196731A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87101075A (en) * | 1987-06-29 | 1988-04-20 | 蒋俊辉 | Whole dyeing technique of one step of plant tissue polychrome and special combination stain thereof |
| CN1360059A (en) * | 2000-12-18 | 2002-07-24 | 拜尔公司 | Method for specific detection of tumor cell and its precursor in cervix smear by simultaneously measuring at least two different molecular labels |
| CN101395182A (en) * | 2006-03-06 | 2009-03-25 | 西福根有限公司 | Recombinant antibodies for treatment of respiratory syncytial virus infections |
| CN101545911A (en) * | 2008-03-25 | 2009-09-30 | 北京科美东雅生物技术有限公司 | Chemoluminescence immunoassay quantitative measuring kit of cytokeratin 18 and preparation method thereof |
| CN101329230A (en) * | 2008-07-14 | 2008-12-24 | 中国人民解放军第三军医大学 | Improved method for dyeing immunofluorescence cell |
| JP2010148447A (en) * | 2008-12-25 | 2010-07-08 | Canon Inc | Composition for staining retinal tissue and method for staining retinal tissue |
| CN102405238A (en) * | 2009-04-20 | 2012-04-04 | 国立大学法人富山大学 | Antibody cocktail for determining cancer histological type, determination kit and determination method |
| US20100322502A1 (en) * | 2009-06-18 | 2010-12-23 | Olympus Corporation | Medical diagnosis support device, image processing method, image processing program, and virtual microscope system |
| CN102924594A (en) * | 2012-08-03 | 2013-02-13 | 无锡傲锐东源生物科技有限公司 | Anti-tumor-protein-P63 monoclonal antibody and purpose thereof |
Non-Patent Citations (12)
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104447994B (en) * | 2014-12-05 | 2018-05-04 | 福州迈新生物技术开发有限公司 | One plant of tumor suppressor gene p63 monoclonal antibody and its application |
| CN104447994A (en) * | 2014-12-05 | 2015-03-25 | 福州迈新生物技术开发有限公司 | Tumor suppressor gene p63 monoclonal antibody and application thereof |
| CN108918493A (en) * | 2018-07-24 | 2018-11-30 | 中国科学院深圳先进技术研究院 | A kind of pH fluorescent dye, pH fluorescence probe, preparation method and application |
| CN110850094A (en) * | 2019-11-22 | 2020-02-28 | 西安交通大学 | Immunohistochemical double-label single-staining kit and use method and application thereof |
| CN112964877A (en) * | 2021-03-09 | 2021-06-15 | 河南赛诺特生物技术有限公司 | Immunohistochemical multiple staining kit and staining procedure for identifying mantle cell lymphoma |
| CN113295871B (en) * | 2021-07-02 | 2023-07-21 | 河南赛诺特生物技术有限公司 | Cocktail immunohistochemical kit for diagnosing breast cancer |
| CN113295871A (en) * | 2021-07-02 | 2021-08-24 | 河南赛诺特生物技术有限公司 | Cocktail immunohistochemical kit for diagnosing breast cancer |
| CN113762379A (en) * | 2021-09-07 | 2021-12-07 | 福州迈新生物技术开发有限公司 | Method for generating training data based on immunohistochemistry and storage device |
| CN114674644A (en) * | 2021-09-07 | 2022-06-28 | 福州迈新生物技术开发有限公司 | Method for generating training data based on immunohistochemistry and storage device |
| WO2023035728A1 (en) * | 2021-09-07 | 2023-03-16 | 福州迈新生物技术开发有限公司 | Method for generating training data based on immunohistochemistry, and storage device |
| CN113762379B (en) * | 2021-09-07 | 2022-06-07 | 福州迈新生物技术开发有限公司 | Method for generating training data based on immunohistochemistry and storage device |
| CN114674644B (en) * | 2021-09-07 | 2024-08-27 | 福州迈新生物技术开发有限公司 | Method and storage device for generating training data based on immunohistochemistry |
| CN116086919A (en) * | 2023-02-20 | 2023-05-09 | 深圳裕策生物科技有限公司 | Staining method and kit for lung cancer and/or pancreatic cancer samples |
| CN116086919B (en) * | 2023-02-20 | 2024-01-26 | 深圳裕策生物科技有限公司 | Staining method and kit for lung cancer and/or pancreatic cancer samples |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103196731A (en) | Multiple stain reagent and detection method for identifying breast myoepithelial lesion | |
| US20210047697A1 (en) | Method for improved diagnosis of dysplasias | |
| CN104977202B (en) | A kind of transmission electron microscope sample preparation method of specimens paraffin embedding slices tissue | |
| CN107202888B (en) | A kind of p16 for cervical liquid-based cellsINK4aImmune labeled colour reagent box | |
| CN101598731B (en) | Immune tissue chemical diagnostic kit used for pathological diagnosis of tumour | |
| US20150309035A1 (en) | Antibody Cocktail Systems and Methods for Classification of Histologic Subtypes in Lung Cancer | |
| CN104237538A (en) | Dairy cow milk progesterone colloidal gold test paper strip and preparation method thereof | |
| WO2023035728A1 (en) | Method for generating training data based on immunohistochemistry, and storage device | |
| WO2014114009A1 (en) | Immunohistochemical quality control reference object and quality control method | |
| JP5370826B2 (en) | Cocktail antibody, discrimination kit and discrimination method for discriminating cancer tissue type | |
| CN110850094A (en) | Immunohistochemical double-label single-staining kit and use method and application thereof | |
| CN106153922B (en) | A kind of colon cancer prognosis prediction mark and its detection method | |
| CN102435728A (en) | Preparation method of positive reference substance for quality inspection and control in immunohistochemical process | |
| CN103471903B (en) | The collocation method of section immobile liquid and application thereof | |
| CN106053806A (en) | HPV (human papillomavirus) immune colloidal gold diagnostic test strip, method for preparing same and detection method implemented by HPV immune colloidal gold diagnostic test strip | |
| WO2014117332A1 (en) | Method for determining sensitivity and affinity of secondary antibody color appearance system for immunohistochemistry | |
| CN105891511A (en) | Probe and kit for identifying circulating tumor cells and neutrophils | |
| JP2021517242A (en) | How to detect MAGEA4 | |
| CN106397583A (en) | POCT (point-of-care testing) fluorescent quantitative test kit for HPV16 type E7 protein and application thereof | |
| CN101738474B (en) | Combined test reagent card for cytomegalovirus and rubella virus | |
| Okazaki et al. | CD146 and insulin‐like growth factor 2 mRNA‐binding protein 3 predict prognosis of asbestos‐induced rat mesothelioma | |
| CN108957004B (en) | Application of reagent for detecting expression levels of H3K9me2 and H3K36me3 in preparation of gastric cancer prognosis evaluation kit | |
| CN113567677B (en) | Immunofluorescence signal amplification technology marking method | |
| CN111929122B (en) | Antigen repairing method for immunocytochemical staining, cell suspension and chemical staining method using same | |
| JP3222251U (en) | Immunodiafiltration apparatus for detecting serum fucose protein |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C05 | Deemed withdrawal (patent law before 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130710 |