CN103160437A - Method for producing microbial granular culture medium - Google Patents
Method for producing microbial granular culture medium Download PDFInfo
- Publication number
- CN103160437A CN103160437A CN2011104196135A CN201110419613A CN103160437A CN 103160437 A CN103160437 A CN 103160437A CN 2011104196135 A CN2011104196135 A CN 2011104196135A CN 201110419613 A CN201110419613 A CN 201110419613A CN 103160437 A CN103160437 A CN 103160437A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- substratum
- rolling
- granular
- microbe granular
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for producing microbial granular culture medium. The method comprises steps of ball mill mixing, mesh sieving, rolling into pieces and crushing. An ordinary microbial culture medium is in a powder shape; and powdered medium generates dust pollution in a production process to hazard the health of production personnel, and the powdered medium agglomerates and condenses in water, and has slow dissolution rate. The method for producing microbial granular culture medium disclosed by the invention produces a granular microbial culture medium, which does not generate dust pollution or affect the health of production personnel; and the granular culture medium does not agglomerate or condense in water, and is easy to dissolve.
Description
Technical field
The present invention relates to a kind of production method of microbiological culture media, relate to specifically a kind of production method of microbe granular substratum.
Background technology
The microbiological culture media of producing at home at present is all Powdered, and powdered medium can produce dust pollution in process of production, and the harm producers' is healthy; And the agglomerate phenomenon of condensing can occur in powdered medium in water, and it is slow that dissolution rate also compares.
Summary of the invention
Technical problem to be solved by this invention just is to provide a kind of method of producing without dust, diffluent microbiological culture media.
The present invention adopts following technical scheme:
A kind of method of producing the microbe granular substratum comprises the steps:
(1) according to culture medium prescription, ball milling in various raw materials input ball grinders is mixed;
(2) substratum after mixing is sieved by screen cloth, remove especially big particle, leave and take the uniform powder of fineness between the 80-120 order;
(3) above-mentioned uniform powder by conveying screw rod send into tablets press roll in wheel rolling in flakes, in flakes rate be 90% or more than;
(4) with Disintegrating knife, above-mentioned slabbing medium powder is broken into particle;
(5) sift out with screen cloth and return to step (4) greater than 80 purpose particles and continue to pulverize, last through the Vibration Screen gumming again less than or equal to 80 purpose particles, collect 20-80 purpose microbe granular substratum.
Further, in step (3), the feeding frequency of conveying screw rod is 8-12Hz, and tablets press is Drygranulatemachine, and it rolls wheel speed is 12-20Hz, and rolling wheel pressure is the 5-12 kilogram, uses cooling water circulation in the operation of rolling.
The invention has the beneficial effects as follows:
Because the microbiological culture media of producing is particulate state, can not produce dust pollution in process of production, guaranteeing can not affect the healthy of producers; The particulate state substratum can not produce the agglomerate phenomenon of condensing yet in water, be easy to dissolving; Adopt Drygranulatemachine can process heat-sensitive material, applied widely.
Embodiment
In order to make purpose of the present invention, technical scheme and advantage clearer, by the following examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, is not intended to limit the present invention.
The invention discloses a kind of method of producing the microbe granular substratum, comprise the steps:
(1) according to culture medium prescription, ball milling in various raw materials input ball grinders is mixed;
(2) substratum after mixing is sieved by screen cloth, remove especially big particle, leave and take the uniform powder of fineness between the 80-120 order;
(3) above-mentioned uniform powder being sent into tablets press by conveying screw rod rolls in wheel rolling in blocks, in flakes rate be 90% or more than, the feeding frequency of above-mentioned conveying screw rod is 8-12Hz, tablets press is Drygranulatemachine, it rolls wheel speed is 12-20Hz, rolling wheel pressure is the 5-12 kilogram, uses cooling water circulation in the operation of rolling;
(4) with Disintegrating knife, above-mentioned slabbing medium powder is broken into particle;
(5) sift out with screen cloth and return to step (4) greater than 80 purpose particles and continue to pulverize, last through the Vibration Screen gumming again less than or equal to 80 purpose particles, collect 20-80 purpose microbe granular substratum.
At last the microbe granular substratum of collecting is carried out bottled or packed getting final product.
Claims (2)
1. a method of producing the microbe granular substratum, is characterized in that, comprises the steps:
(1) according to culture medium prescription, ball milling in various raw materials input ball grinders is mixed;
(2) substratum after mixing is sieved by screen cloth, remove especially big particle, leave and take the uniform powder of fineness between the 80-120 order;
(3) above-mentioned uniform powder by conveying screw rod send into tablets press roll in wheel rolling in flakes, in flakes rate be 90% or more than;
(4) with Disintegrating knife, above-mentioned slabbing medium powder is broken into particle;
(5) sift out with screen cloth and return to step (4) greater than 80 purpose particles and continue to pulverize, last through the Vibration Screen gumming again less than or equal to 80 purpose particles, collect 20-80 purpose microbe granular substratum.
2. the method for production microbe granular substratum according to claim 1; it is characterized in that: in step (3), the feeding frequency of conveying screw rod is 8-12Hz; tablets press is Drygranulatemachine; it rolls wheel speed is 12-20Hz; rolling wheel pressure is the 5-12 kilogram, uses cooling water circulation in the operation of rolling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104196135A CN103160437A (en) | 2011-12-15 | 2011-12-15 | Method for producing microbial granular culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104196135A CN103160437A (en) | 2011-12-15 | 2011-12-15 | Method for producing microbial granular culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103160437A true CN103160437A (en) | 2013-06-19 |
Family
ID=48583973
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104196135A Pending CN103160437A (en) | 2011-12-15 | 2011-12-15 | Method for producing microbial granular culture medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103160437A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105010817A (en) * | 2015-06-25 | 2015-11-04 | 韶关朗润环保科技有限公司 | Special microbial cultivation feed for domestic sewage and production technology of special microbial cultivation feed |
| CN105727335A (en) * | 2016-02-16 | 2016-07-06 | 成都晓鱼文化传播有限公司 | Making process of pure-natural and additive-free incense material |
| CN106399102A (en) * | 2016-09-14 | 2017-02-15 | 青岛佳明测控科技股份有限公司 | Simple preparation method of microorganism particle culture medium |
| CN109136091A (en) * | 2018-09-06 | 2019-01-04 | 北京三药科技开发公司 | A kind of tablet culture medium and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002036735A2 (en) * | 2000-11-06 | 2002-05-10 | Invitrogen Corporation | Dry powder cells and cell culture reagents and methods of production thereof |
| CN1554743A (en) * | 2003-12-22 | 2004-12-15 | 佩 胡 | Thuringinensin bacillus solid pelletizing and cultivating method |
| CN1699555A (en) * | 2004-05-20 | 2005-11-23 | 上海旭太生物工程有限公司 | Process for preparing powder type animal cell culture medium |
-
2011
- 2011-12-15 CN CN2011104196135A patent/CN103160437A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002036735A2 (en) * | 2000-11-06 | 2002-05-10 | Invitrogen Corporation | Dry powder cells and cell culture reagents and methods of production thereof |
| CN1554743A (en) * | 2003-12-22 | 2004-12-15 | 佩 胡 | Thuringinensin bacillus solid pelletizing and cultivating method |
| CN1699555A (en) * | 2004-05-20 | 2005-11-23 | 上海旭太生物工程有限公司 | Process for preparing powder type animal cell culture medium |
Non-Patent Citations (2)
| Title |
|---|
| D JAYME ET AL.: "A NOVEL APPLICATION OF GRANULATION TECHNOLOGY TO IMPROVE PHYSICAL PROPERTIES AND BIOLOGICAL PERFORMANCE OF POWDERED SERUM-FREE CULTURE MEDIA", 《ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS》, vol. 12, 31 December 2002 (2002-12-31) * |
| RICHARD FIKE ET AL.: "Advanced Granulation Technology (AGTTM) An alternate format for serum-free, chemically-defined and protein-free cell culture media", 《CYTOTECHNOLOGY》, vol. 36, 31 December 2001 (2001-12-31) * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105010817A (en) * | 2015-06-25 | 2015-11-04 | 韶关朗润环保科技有限公司 | Special microbial cultivation feed for domestic sewage and production technology of special microbial cultivation feed |
| CN105727335A (en) * | 2016-02-16 | 2016-07-06 | 成都晓鱼文化传播有限公司 | Making process of pure-natural and additive-free incense material |
| CN106399102A (en) * | 2016-09-14 | 2017-02-15 | 青岛佳明测控科技股份有限公司 | Simple preparation method of microorganism particle culture medium |
| CN109136091A (en) * | 2018-09-06 | 2019-01-04 | 北京三药科技开发公司 | A kind of tablet culture medium and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104845411B (en) | Extra fine heavy calcium carbonate powder body production method | |
| CN103160437A (en) | Method for producing microbial granular culture medium | |
| CN103481392A (en) | Rubber processing method | |
| CN105315022A (en) | Method for humate pelletization through dry process | |
| CN104962335A (en) | Biomass briquette preparation method | |
| CN101721949A (en) | Method for granulating powdery active lime, special equipment and midbody | |
| CN204564261U (en) | A kind of integral type for traditional Chinese powder medicine production crushes and screens device | |
| CN106007733A (en) | Preparation method of ceramic powder | |
| CN104589543A (en) | Waste tire cooling deep processing treatment method | |
| CN204638303U (en) | A kind of powdery former medicine production micron ultra shifter | |
| TW202023667A (en) | Water purifying agent and water purifying method | |
| CN204865754U (en) | Dry process roll -in granulator | |
| CN201728085U (en) | Novel aluminum alloy refining particle flux granulator | |
| CN103588194A (en) | Graphite ball production device based on grader and processing method of graphite ball production device | |
| CN101289201A (en) | Method for preparing high-concentration round grainy potassium muriate | |
| CN101823150A (en) | Method for preparing reduced iron powder | |
| CN202666967U (en) | Raw material grinding and processing device | |
| CN103923494B (en) | The preparation method of lamp box advertisement film specialized superfine hydrated magnesium silicate powder | |
| CN202823513U (en) | Energy-saving cement ball mill | |
| CN203076007U (en) | Double-roller crusher in soy sauce production | |
| CN102198383A (en) | Compound desulfurizer | |
| CN101810330A (en) | Enteromorpha clathrata-dendrobium refined flour | |
| CN107837760A (en) | A kind of MOX powder method of granulating | |
| CN202492473U (en) | Disk granulator | |
| CN102370138B (en) | Clean production method of infant formula rice flour |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130619 |