CN103155809B - Method of culturing tomato grafted seedling - Google Patents
Method of culturing tomato grafted seedling Download PDFInfo
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- CN103155809B CN103155809B CN201310128857.7A CN201310128857A CN103155809B CN 103155809 B CN103155809 B CN 103155809B CN 201310128857 A CN201310128857 A CN 201310128857A CN 103155809 B CN103155809 B CN 103155809B
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- cutting
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- tomato
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- 235000007688 Lycopersicon esculentum Nutrition 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000012258 culturing Methods 0.000 title abstract 3
- 240000003768 Solanum lycopersicum Species 0.000 title description 21
- 238000005520 cutting process Methods 0.000 claims abstract description 29
- 241000196324 Embryophyta Species 0.000 claims abstract description 23
- 244000061458 Solanum melongena Species 0.000 claims abstract description 18
- 235000002597 Solanum melongena Nutrition 0.000 claims abstract description 18
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims abstract description 5
- 241000238631 Hexapoda Species 0.000 claims abstract description 5
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000006013 carbendazim Substances 0.000 claims abstract description 5
- 201000010099 disease Diseases 0.000 claims abstract description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 5
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims abstract description 5
- 241000227653 Lycopersicon Species 0.000 claims abstract 7
- 240000002072 Solanum torvum Species 0.000 claims description 9
- 235000013358 Solanum torvum Nutrition 0.000 claims description 9
- 238000009395 breeding Methods 0.000 claims description 9
- 230000008774 maternal effect Effects 0.000 claims description 4
- 239000011159 matrix material Substances 0.000 claims description 4
- 238000003825 pressing Methods 0.000 claims description 4
- 241000607479 Yersinia pestis Species 0.000 abstract 1
- 230000034303 cell budding Effects 0.000 abstract 1
- 238000003306 harvesting Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
- 239000000843 powder Substances 0.000 description 5
- 230000018044 dehydration Effects 0.000 description 3
- 238000006297 dehydration reaction Methods 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 2
- 230000002073 mitogenetic effect Effects 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method of culturing a tomato grafted seedling. The method includes the following steps of conducting cuttage on a lateral branch of a tomato elderly plant, using a new bud obtained through cuttage as a scion, using a wild eggplant as a rootstock, grafting the rootstock and the scion, cultivating the grafted seedlings, selecting good and robust plants without plant diseases and insect pests as stock plants, picking a lateral branch with 3 leaves to 5 leaves and using the lateral branch as a cutting, cutting leaves, budding inflorescence and growing points of the picked cutting, simultaneously cutting 4 centimeters of the lower portion of the cutting, wherein a cutting opening of the lower end needs to be smooth and an angle of the cutting opening is from 30 degrees to 45 degrees, soaking the well cut cutting in 800-time carbendazim solution for 1 minute to disinfect the cutting, and simultaneously preventing the cutting from loosing water and rootage from being influenced. The lower portion of the cutting is soaked in 100 milligrams per kilogram of indolebutyric acid for 10 seconds to 15 seconds, and therefore high rooting percentage can be obtained and rooting time can be shortened. The cutting is inserted into base material with 5cm*5cm density, the inserting depth is from 2 centimeters to 3 centimeters, a root system and a new bud come up after 10 days to 15 days, and the new bud is cut out to be used as the scion when 3 leaves to 4 leaves grow on the new bud. The method of culturing the tomato grafted seedling has the advantages of being high in uniformity, easy to manage after planting, high in harvest and consistent in fructescence.
Description
Technical field
The breeding method that the present invention relates to a kind of Tomato Grafting seedling, belongs to agricultural technology field.
Background technology
Tomato plant axillalry bud is more and all can form fruit bearing shoot, can utilize high-order axillalry bud (side shoot that after tomato result, plant grows) to carry out graft seedling growth do scion.The method has reduced seed expenditure, has reduced production cost.But because the axillalry bud that after tomato result, plant grows is not of uniform size, cause grafting size uneven, regularity is low, strengthen the field management difficulty after field planting, yield poorly, the listing phase is inconsistent.
Summary of the invention
Technical problem to be solved by this invention is for above problem, and a kind of breeding method of Tomato Grafting seedling is provided, and adopts the method can guarantee grafting neat and consistent, and regularity can to do the grafting of scion suitable with seed bud.
For addressing the above problem, the present invention takes following technical scheme: a kind of breeding method of Tomato Grafting seedling, described breeding method comprises the following steps: the side shoot of the old strain of tomato is carried out to cuttage, the sprouting that cuttage obtains does scion, wildness eggplant is stock, grafting is carried out in stock and scion, cultivates grafting.
Further improvement as technique scheme:
The cultivating process of described scion comprises the following steps:
1) select good, healthy and strong, do maternal plant without the plant of damage by disease and insect, winning long has the side shoot of 3-5 sheet leaf size to make cuttings.
2) by the blade of adopted cuttings and the inflorescence of having buddingged and growing point excision, cut off bottom 4cm, lower end otch requires level and smooth simultaneously, and cut angle is 30-45 degree.
3) cuttings cuts rear entirety and puts into 800 times of carbendazim solutions and soak and carry out disinfection for 1 minute, also prevents dehydration simultaneously, and impact is taken root.10-15 second is soaked in cuttings bottom with 100mg/kg indolebutyric acid, can obtain higher rooting rate and shorten rootage duration.
4) density of pressing 5cm × 5cm is inserted matrix, inserts dark 2-3cm.
5) after 10-15 days, can grow root system and sprouting, when sprouting grows to 3-4 sheet leaf, intercept and do scion.
Described stock and scion adopt cleft graft to carry out grafting.
Described wildness eggplant is African red eggplant.
Described wildness eggplant is Solanum Torvum.
Adopt above technical scheme, compared with prior art, have the following advantages: the Tomato Grafting seedling regularity that adopts the method to cultivate is high, manageability after field planting, output is high, and the fructescence is consistent.
Below in conjunction with embodiment, the invention will be further described.
Embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
Embodiment 1, field trial is at ancient city street, Shouguang City of Shandong Province Vegetable Base.Varieties of plant is Provence tomato, carries out grafting cultivation with wildness eggplant African red eggplant as stock, field planting on July 25.
3 processing are established in test, are respectively side shoot secondary sprouting grafting, plant bud grafting and lateral-branch grafting seedling.Repeat for 3 times, adopt random district group to arrange, community area 50m
2, every community field planting 120 strains.Control measures are identical.
Process 1: the side shoot of the old strain of tomato is carried out to cuttage, and the sprouting that cuttage obtains does scion, and African red eggplant is stock, and grafting is carried out in stock and scion, cultivate grafting.
The cultivating process of described scion comprises the following steps:
1) select good, healthy and strong, do maternal plant without the powder Jiamei tomato plant of damage by disease and insect, winning long has the side shoot of 3-5 sheet leaf size to make cuttings.
2) by all blades of adopted cuttings and the inflorescence of having buddingged and growing point excision, cut off bottom 4cm, lower end otch requires level and smooth simultaneously, and in practical operation, cut angle is difficult to accomplish unified angle, is generally 30-45 degree.
3) cuttings cuts rear entirety and puts into 800 times of carbendazim solutions and soak and carry out disinfection for 1 minute, also prevents dehydration simultaneously, and impact is taken root.10-15 second is soaked in cuttings bottom with 100mg/kg indolebutyric acid, can obtain higher rooting rate and shorten rootage duration.
4) density of pressing 5cm × 5cm is inserted matrix, inserts dark 2-3cm.
5) after 10-15 days, can grow root system and sprouting, when sprouting grows to 3-4 sheet leaf, can intercept and do scion.
When African red eggplant grows to 5-6 sheet true leaf, stay 2 true leaves of base portion to go to top, intercept the secondary sprouting of 3-4 sheet leaf size, retain 2 leaf 1 hearts, adopt cleft graft to carry out grafting.
Process 2: plant bud grafting.Cultivate scion with Provence tomato seeds, African red eggplant is that stock is cultivated grafting.When African red eggplant seedling grows to 5-6 sheet true leaf, stay 2 true leaves of base portion to go to top, scion cuts top 2 leaf 1 heart parts, with the grafting of stock cleft graft.
Process 3: lateral-branch grafting seedling.After the tomato result of Provence, the mitogenetic axillalry bud in plant high joint position is done scion, and African red eggplant is that stock is cultivated grafting.When African red eggplant grows to 5-6 sheet true leaf, stay 2 true leaves of base portion to go to top, intercept high-order axillalry bud 2 leaf 1 heart parts and do scion, cleft graft grafting.
Result shows: it is 1.82 × 10 that tomato lateral branch cutting secondary sprouting grafting is processed output
5kghm
-2, plant bud grafting and be treated to 1.83 × 10
5kghm
-2, lateral-branch grafting seedling is treated to 1.69 × 10
5kghm
-2, the former two's volume variance is not remarkable, but reaches significance level with the latter's difference.
Embodiment 2, field trial is at ancient city street, Shouguang City of Shandong Province Vegetable Base.Varieties of plant is powder Jiamei tomato, carries out grafting cultivation with wildness eggplant Solanum Torvum as stock, field planting on July 25.
3 processing are established in test, are respectively side shoot secondary sprouting grafting, plant bud grafting and lateral-branch grafting seedling.Repeat for 3 times, adopt random district group to arrange, community area 50m
2, every community field planting 120 strains.Control measures are identical.
Process 1: the side shoot of the old strain of tomato is carried out to cuttage, and the sprouting that cuttage obtains does scion, and Solanum Torvum is stock, and grafting is carried out in stock and scion, cultivate grafting.
The cultivating process of described scion comprises the following steps:
1) select good, healthy and strong, do maternal plant without the powder Jiamei tomato plant of damage by disease and insect, winning long has the side shoot of 3-5 sheet leaf size to make cuttings.
2) by all blades of adopted cuttings and the inflorescence of having buddingged and growing point excision, cut off bottom 4cm, lower end otch requires level and smooth simultaneously, and in practical operation, cut angle is difficult to accomplish unified angle, is generally 30-45 degree.
3) cuttings cuts rear entirety and puts into 800 times of carbendazim solutions and soak and carry out disinfection for 1 minute, also prevents dehydration simultaneously, and impact is taken root.10-15 second is soaked in cuttings bottom with 100mg/kg indolebutyric acid, can obtain higher rooting rate and shorten rootage duration.
4) density of pressing 5cm × 5cm is inserted matrix, inserts dark 2-3cm.
5) after 10-15 days, can grow root system and sprouting, when sprouting grows to 3-4 sheet leaf, can intercept and do scion.
When Solanum Torvum grows to 5-6 sheet true leaf, stay 2 true leaves of base portion to go to top, intercept the secondary sprouting of 3-4 sheet leaf size, retain 2 leaf 1 hearts, adopt cleft graft to carry out grafting.
Process 2: plant bud grafting.With the cultivating seeds scion of powder Jiamei, Solanum Torvum is that stock is cultivated grafting.When Solanum Torvum seedling grows to 5-6 sheet true leaf, stay 2 true leaves of base portion to go to top, scion cuts top 2 leaf 1 heart parts, with the grafting of stock cleft graft.
Process 3: lateral-branch grafting seedling.After powder Jiamei tomato result, the mitogenetic axillalry bud in plant high joint position is done scion, and Solanum Torvum is that stock carries out graft seedling growth.When Solanum Torvum grows to 5-6 sheet true leaf, stay 2 true leaves of base portion to go to top, intercept high-order axillalry bud 2 leaf 1 heart parts and do scion, cleft graft grafting.
Result shows: it is 1.82 × 10 that tomato side shoot secondary sprouting grafting is processed output
5kghm
-2, plant bud grafting and be treated to 1.83 × 10
5kghm
-2, lateral-branch grafting seedling is treated to 1.69 × 10
5kghm
-2, the former two's volume variance is not remarkable, but reaches significance level with the latter's difference.
Because the growing state of different plants has difference, in practical operation, be difficult to accomplish that actual conditions is all unified, so some condition has difference, such as the degree of depth of step 4) and the quantity of blade can be reasonably selecting to implement in scope, and do not affect experimental result.
The above is giving an example of best mode for carrying out the invention, and the part of wherein not addressing is in detail those of ordinary skills' common practise.Protection scope of the present invention is as the criterion with the content of claim, and any equivalent transformation carrying out based on technology enlightenment of the present invention, also within protection scope of the present invention.
Claims (3)
1. a breeding method for Tomato Grafting seedling, is characterized in that: described breeding method comprises the following steps:
The side shoot of the old strain of tomato is carried out to cuttage, and the sprouting that cuttage obtains does scion, and wildness eggplant is stock, and grafting is carried out in stock and scion, cultivates grafting;
The cultivating process of described scion comprises the following steps:
1) select good, healthy and strong, do maternal plant without the plant of damage by disease and insect, winning long has the side shoot of 3-5 sheet leaf size to make cuttings;
2) by the blade of adopted cuttings and the inflorescence of having buddingged and growing point excision, cut off bottom 4cm, lower end otch requires level and smooth simultaneously, and cut angle is 30-45 degree;
3) cuttings cuts rear entirety and puts into 800 times of carbendazim solutions and soak 1 minute, with 100mg/kg indolebutyric acid, 10 seconds is soaked in cuttings bottom afterwards;
4) density of pressing 5cm × 5cm is inserted matrix, inserts dark 2-3cm;
5) after 10-15 days, can grow root system and sprouting, when sprouting grows to 3-4 sheet leaf, intercept and do scion;
Described stock and scion adopt cleft graft to carry out grafting.
2. a kind of breeding method of Tomato Grafting seedling according to claim 1, is characterized in that: described wildness eggplant is African red eggplant.
3. a kind of breeding method of Tomato Grafting seedling according to claim 1, is characterized in that: described wildness eggplant is Solanum Torvum.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310128857.7A CN103155809B (en) | 2013-04-15 | 2013-04-15 | Method of culturing tomato grafted seedling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310128857.7A CN103155809B (en) | 2013-04-15 | 2013-04-15 | Method of culturing tomato grafted seedling |
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| CN103155809A CN103155809A (en) | 2013-06-19 |
| CN103155809B true CN103155809B (en) | 2014-05-21 |
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Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103416230A (en) * | 2013-08-27 | 2013-12-04 | 遵义市龙泉农业科技发展有限公司 | Disease-resistant, draught-resistant and cold-resistant grafting technology for black tomatoes in South America |
| CN103975758B (en) * | 2014-06-06 | 2016-06-22 | 寿光市永盛种子有限公司 | A kind of method utilizing color sweet pepper axillalry bud to cultivate grafting |
| CN103975759B (en) * | 2014-06-06 | 2016-06-29 | 寿光市永盛种子有限公司 | A kind of method utilizing Fructus Lycopersici esculenti axillalry bud to cultivate grafting |
| CN104303848A (en) * | 2014-07-01 | 2015-01-28 | 上海采科实业有限公司 | Tomato and eggplant symbiotic plant |
| CN104813850A (en) * | 2015-03-31 | 2015-08-05 | 广西大学 | Grafting method for tomato |
| CN105532267A (en) * | 2015-12-07 | 2016-05-04 | 崔学晨 | Ornamental tree-shape tomato self-strain stem-vine pup joint anti-premature senility method |
| CN105993623A (en) * | 2016-05-23 | 2016-10-12 | 句容市绿润苗木有限公司 | Grafting method for cherry tomatoes |
| CN106171377A (en) * | 2016-06-30 | 2016-12-07 | 百色阳生农业开发有限公司 | A kind of cultural method improving outdoor cropping Tomato Grafting Seedling yield |
| CN106561302A (en) * | 2016-10-25 | 2017-04-19 | 王伟 | Tomato grafting method |
| CN107278566A (en) * | 2017-06-30 | 2017-10-24 | 宁夏天瑞产业集团现代农业有限公司 | A kind of tomato lateral branch cutting fast breeding method |
| CN109588225A (en) * | 2019-01-23 | 2019-04-09 | 四川农业大学 | A kind of engrafting method for significantly improving tomato and resisting high cadmium soil Cd stress ability |
| CN110972742A (en) * | 2019-10-24 | 2020-04-10 | 湖南省蔬菜研究所 | Method for advancing autumn delay and later tomato seedling period by utilizing Guangdong-fit eggplant stock |
| CN111742729A (en) * | 2020-07-03 | 2020-10-09 | 南京农业大学 | Method for rapid propagation of high-quality tomato seedlings |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100576996C (en) * | 2007-11-20 | 2010-01-06 | 丁加刚 | Method for cultivating tomato stock using resistant variety axillary buds cuttage |
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Denomination of invention: A cultivation method for tomato grafted seedlings Granted publication date: 20140521 Pledgee: Weifang Bank Co.,Ltd. Xincheng sub branch Pledgor: SHANDONG SHOUGUANG VEGETABLE INDUSTRY GROUP Co.,Ltd. Registration number: Y2024980033445 |