CN103142683B - Be used for the treatment of Chinese medicinal perfusion liquid containing Herba Sophorae alopecuroidis total alkali of bovine mastitis and endometritis and preparation method thereof - Google Patents
Be used for the treatment of Chinese medicinal perfusion liquid containing Herba Sophorae alopecuroidis total alkali of bovine mastitis and endometritis and preparation method thereof Download PDFInfo
- Publication number
- CN103142683B CN103142683B CN201310079272.0A CN201310079272A CN103142683B CN 103142683 B CN103142683 B CN 103142683B CN 201310079272 A CN201310079272 A CN 201310079272A CN 103142683 B CN103142683 B CN 103142683B
- Authority
- CN
- China
- Prior art keywords
- herba sophorae
- sophorae alopecuroidis
- total alkali
- alopecuroidis total
- chinese medicinal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses one and be used for the treatment of milch cow pathogenicity mastitis and endometritis, the Chinese medicinal perfusion liquid containing Herba Sophorae alopecuroidis total alkali of effective fast, low, the easy metabolism of toxicity, noresidue, and provide its preparation method.Beneficial effect of the present invention is: Herba Sophorae alopecuroidis total alkali infusion liquid, as pure natural medical, has toxic and side effects little, and the advantage such as not easily develop immunity to drugs, cost can be saved significantly, reduce and abandon milk loss, and herbal treatment focuses on conditioning, is conducive to the recovery of animal economy function and body condition.The Chinese medicinal perfusion liquid of this kind containing Herba Sophorae alopecuroidis total alkali has vast potential for future development for treatment bovine mastitis.
Description
Technical field
The present invention relates to application in TCM field, be specifically related to the Chinese medicinal perfusion liquid containing Herba Sophorae alopecuroidis total alkali being used for the treatment of bovine mastitis and endometritis.
Background technology
Herba Sophorae alopecuroidis is perennial leguminous herbaceous plant, the dry herb of Herba Sophorae alopecuroidis and seed.Herba Sophorae alopecuroidis is distributed widely in the ground such as Gansu, Ningxia, Xinjiang, Tibet, the Inner Mongol.Herb is gathered summer, and seed is gathered spring, dry.The raw use of herb, seed is fried and is used.In recent years country by Herba Sophorae alopecuroidis one of six large authentic medicinal herbses listing focused protection in, include in national modernization of Chinese medicine scientific and technological industry action plan, have significant progress for construction of production base and deep level development.Herba Sophorae alopecuroidis taste is extremely bitter, cold in nature poisonous, has heat-clearing and toxic substances removing, the effects such as anti-inflammation, pain relieving parasite killing.Laryngalgia, cough, dysentery and eczema etc. are treated in radical cure among the people.8 kinds of alkaloids are mainly contained: sophocarpine, matrine, oxymatrine, N-oxysophocarpine one, sophoramine, sophoridine, lehmannine, aloperine at present for drug development.The pharmacological action of Alkaloids From Sophora Alopecuroides L: 1, antibacterial action, has successfully developed treatment dysentery medicine Herba Sophorae alopecuroidis sheet and treatment gynecological inflammation medicine anti-inflammatory suppository for gynecopathy (Herba Sophorae alopecuroidis) bolt at present; 2, to the effect of cardiovascular system, arrhythmia, positive inotropic action is embodied in, blood pressure lowering and reduce heart rate function, to the protective effect of myocardial ischemia and infraction, effect for reducing blood fat; 3, antiviral, antiinflammatory and immunosuppressive action, Herba Sophorae alopecuroidis total alkali can suppress multiple virus breeding. strengthen immunoloregulation function and immunologic enhancement; 4, antitumor action, obvious to hepatoma carcinoma cell inhibitory action, also there is inhibitory action to cervical cancer, lymphosarcoma, pulmonary carcinoma; 5, to the effect of smooth muscle, Herba Sophorae alopecuroidis total alkali and some monosomic alkali all can resist the Contraction of The Ileum From A White of being induced by inflammatory mediator, to bronchial smooth muscle, there is relexation, also effect is had: 6, clinical practice Herba Sophorae alopecuroidis extractum sheet, aloperine treat acute bacillary dysentery at present to block mold, sophocarpine sheet treatment bronchial asthma, Sophorcarpidine Treating gynecological inflammation, Treated by Oxymatrine Injection chronic viral hepatitis B all obtain good curative effect.
Bovine mastitis and endometritis are a kind of diseases that is the most common in world's dairy and that cause economic loss maximum, its pathogenic factor mainly cause pathogeny imcrobe infection.Cause the pathogenic microorganism of bovine mastitis and endometritis very complicated, comprise antibacterial, fungus, virus, mycoplasma etc.Wherein based on bacteriological infection, mainly contain staphylococcus aureus, escherichia coli, streptococcus agalactiae, staphylococcus epidermidis.Next is fungal infection, and usually mixed infection.The generation of this disease directly affects the Quality and yield of milk, also can reduce the time limit of giving milk of milch cow, add mortality.High, the difficult control of sickness rate clinically of this disease, causes huge economic loss to milk cow production.
A lot, as anti-inflammation, enhancing body resistance, and with vaccine immunity and good management raising etc., but bovine mastitis and endometritis incidence probability are still high especially for current bovine mastitis and endometritis prophylactico-therapeutic measures.Particularly the clinical antibiotic that generally adopts and chemical medicine treatment bovine mastitis and endometritis, be first the clinical symptom disappearance of bovine mastitis and endometritis, but be easy to recurrence; Secondly antibiotic and the antibacterial drug resistance ubiquity of chemical medicine, use the dosage of antibiotic and chemical medicine continuing to increase, but clinical efficacy is bad, adds antibiotic remains, direct harm humans is healthy.
Summary of the invention
Object of the present invention is exactly for above-mentioned defect of the prior art, provides one and is used for the treatment of milch cow pathogenicity mastitis and endometritis, the Chinese medicinal perfusion liquid of effective fast, low, the easy metabolism of toxicity, noresidue.
To achieve these goals, technical scheme provided by the invention is: a kind of Chinese medicinal perfusion liquid, containing Herba Sophorae alopecuroidis total alkali in described Chinese medicinal perfusion liquid.
Further, above-mentioned Chinese medicinal perfusion liquid, containing Herba Sophorae alopecuroidis total alkali 3-8g in described Chinese medicinal perfusion liquid.
Further, above-mentioned Chinese medicinal perfusion liquid, containing Herba Sophorae alopecuroidis total alkali 6g in described Chinese medicinal perfusion liquid.
Second object of the present invention there is provided a kind of preparation method of Chinese medicinal perfusion liquid, comprises the following steps:
1) get Herba Sophorae alopecuroidis seed meal and be broken to 50-80 order, adding percent by volume is that the ethanol water of 50%-90% carries out ultrasonic extraction, supersonic frequency 20KHz, 5000W, reclaims ethanol after filtration, adds 2% hydrochloric acid and adjusts pH to be 2-5, add washed with diethylether 2 times, obtain filtrate precipitation, then add chloroform extraction three precipitations, reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali;
2) add water for injection standardize solution in the Herba Sophorae alopecuroidis total alkali obtained in step 1), the mass ratio of Herba Sophorae alopecuroidis total alkali and water for injection is (3-8): (92-97), adopts flowing steam sterilization 30-40min, aseptically carries out fill and obtain finished product Chinese medicinal perfusion liquid.
Further, above-mentioned preparation method, described step 2) in, the mass ratio of Herba Sophorae alopecuroidis total alkali and water for injection is 6:94.
3rd object of the present invention there is provided the application of Herba Sophorae alopecuroidis total alkali in the Chinese medicinal perfusion liquid for the preparation for the treatment of bovine mastitis and endometritis.
Further, above-mentioned application, the use amount of described Chinese medicinal perfusion liquid is each newborn district 30-60ml of every cow head.
Beneficial effect of the present invention is: the Chinese medicinal perfusion liquid containing Herba Sophorae alopecuroidis total alkali provided by the invention, and compared with Ampicillin Sodium For Injection, the cure rate for the treatment of mammitis of cow is 90% and 70% respectively, and in cure rate, Herba Sophorae alopecuroidis total alkali infusion liquid is higher.In economic benefit, Ampicillin Sodium For Injection treatments period adds the withdrawal time of 4 days, has that nearly 7d's abandon the milk phase, the health of the remaining influence consumer of medicine, and frequent use easily causes drug resistance, causes again treating more difficult; Herba Sophorae alopecuroidis total alkali infusion liquid, as pure natural medical, has toxic and side effects little, and the advantage such as not easily develop immunity to drugs, and can save cost significantly, reduce and abandon milk loss, and herbal treatment focuses on conditioning, is conducive to the recovery of animal economy function and body condition.The Chinese medicinal perfusion liquid of this kind containing Herba Sophorae alopecuroidis total alkali has vast potential for future development for treatment bovine mastitis.The preparation method of Herba Sophorae alopecuroidis total alkali infusion liquid provided by the invention, process is simple.The invention also discloses the application of extract Herba Sophorae alopecuroidis total alkali infusion liquid in treatment bovine mastitis and endometritis of Herba Sophorae alopecuroidis, this medicine has antibacterial, antiinflammatory, low toxicity, noresidue, not easily produces the advantages such as drug resistance, and zest is little, cost is lower, effectively can treat control and the treatment, particularly targeted therapy bovine mastitis and endometritis of the disease such as bovine mastitis and endometritis because antibacterial, fungus, infusorian cause.The present invention has the extract of single medicinal material, is conducive to stability and the quality controllability of product; Targeting preparation, in cow mammary gland and intrauterine release, reduces gastral absorption, directly discharges and can be scattered in cow breast and uterus by higher concentration at diseased region, more effective, definite to the treatment of disease; Targeting preparation reduces medicine to upper gastrointestinal stimulation, and untoward reaction is few, easy to use, and compliance is good.Dairy farmer and milk cattle cultivating enterprise is made to obtain good economic benefit.
The concrete testing result of the Chinese medicinal perfusion liquid containing Herba Sophorae alopecuroidis total alkali provided by the invention:
One, the mensuration of Herba Sophorae alopecuroidis total bases extract
Herba Sophorae alopecuroidis total alkali, get this product and be about 0.15g, accurately weighed, put in beaker, add hydrochloric acid 0.2mol/L5ml to dissolve, move in separatory funnel, divide with water 10ml and wash beaker for several times, washing liquid and solution merge, add sodium chloride 6g, shake loose, add caustic lye of soda 1mol/l5ml again, extract 5 times with chloroform jolting and be respectively 25ml, 20ml, 10ml, 10ml, 10ml divides the chloroform layer got successively by being plugged with absorbent cotton and filling the glass funnel of 1g anhydrous sodium sulfate, filter in another separatory funnel, combined chloroform liquid, precision adds sulphuric acid volumetric solution 0.05mol/L20ml and water 10ml.After jolting is extracted, separate acid layer, chloroform layer washes three times with water, each 10ml.Merge acid solution and washing liquid, put in conical flask, the chloroform that heating in water bath removing is residual, let cool to room temperature, the red indicator of methylate 2, with sodium hydroxide titration liquid 0.1mol/l titration, every 1ml sulphuric acid volumetric solution 0.05mol/l is equivalent to the C1 of 24.84mg
5h
21n
2o.
Sophoridine measures according to high performance liquid chromatography Chinese Pharmacopoeia version in 2000 two annex VID.
Chromatographic condition and system suitability: chromatographic column, Spherisorb nh 2 column; With acetonitrile-phosphate aqueous solution PH2-dehydrated alcohol 80:8:10 for mobile phase; Determined wavelength 220nm; The separating degree of sophoridine and related alkaloids should meet the requirements; Relative standard deviation should be less than 2.0%.
Algoscopy: get this product 25mg, accurately weighed, put in 100ml measuring bottle, dissolve with acetonitrile-dehydrated alcohol 8:1 and be diluted to scale, shaking up.Precision measures 20 μ l injection liquid chromatographies, record chromatogram; Separately get sophoridine reference substance and be about 10mg, accurately weighed, put in 100ml measuring bottle, be diluted to scale by above-mentioned dissolution with solvents, shake up, be measured in the same method.Sophoridine C in test sample is gone out with calculated by peak area by external standard method
15h
24n
2the content of O.
Two, specific experiment process:
(1) Odontothrips loti measures the antibacterial activity in vitro of total alkaloid of sophora alopecuroide
Odontothrips loti is adopted to determine the antibacterial activity of Herba Sophorae alopecuroidis total alkali to escherichia coli, staphylococcus aureus, staphylococcus epidermidis, streptococcus agalactiae.Result of the test shows, when Herba Sophorae alopecuroidis total alkali concentration is 5mg/ml, is respectively 9.94mm, 10.65mm, 10.66mm, 6.89 mm to the antibacterial circle diameter of escherichia coli, staphylococcus aureus, staphylococcus epidermidis, streptococcus agalactiae.When 20mg/ml, antibacterial circle diameter is respectively 19.30 mm, 13.98 mm, 19.69 mm, 12.92 mm.The rising along with concentration can be found out, Herba Sophorae alopecuroidis total alkali to the inhibitory action of escherichia coli and staphylococcus epidermidis apparently higher than to staphylococcus aureus and streptococcus agalactiae.Wherein the strongest to the staphylococcic inhibitory action of epidermis, the most weak relative to other three kinds of bacterium to the inhibitory action of streptococcus agalactiae.
1, materials and methods
1.1, material and reagent
For examination bacterium: staphylococcus aureus, escherichia coli, streptococcus agalactiae, staphylococcus epidermidis, provide by Lanzhou herding and veterinary drug institute veterinary drug research department.
For sample: Herba Sophorae alopecuroidis total alkali, produced by Hinapharm Pharmaceutical Co., Ltd., with the test liquid being mixed with variable concentrations after water dissolution.
1.2, instrument and equipment
KH-300DB type numerical control ultrasonic cleaner, Kunshan He Chuan ultrasonic instrument company limited; RE-201D Rotary Evaporators, Yuhua Instrument Co., Ltd., Gongyi City; Electro-heating standing-temperature cultivator, Shanghai Hong Dou Electronic Science and Technology Co., Ltd. DNP-9272A type; Constant temperature oscillator, Jing Qi company limited of U.S. IS-RDV1; Biohazard Safety Equipment, Shanghai Shen Li scientific instrument company limited HFsafe-1500.
1.3, the preparation of bacteria suspension
Bacterium liquid good for activation culture is accessed in physiological saline solution respectively, carries out gradient dilution successively, make the bacterial population in bacteria suspension be 107cfu/ml.
1.4, Odontothrips loti measures antibacterial activity
The culture medium 20ml melted is added in culture dish that autoclaving crosses, solidifying rear absorption 200 microlitre bacteria suspension adds wherein, with spreader by even for the coating of bacterium liquid, make the plate that carries disease germs, with the Oxford cup that aseptic nipper gripping high pressure steam sterilization is also dried, be flat on the culture dish surface being coated with bacterium liquid gently, each culture dish cruciform symmetry places 4 Oxford cups, the sample of 200 microlitre different quality concentration is pipetted respectively in the cup of Oxford with aseptic liquid-transfering gun, culture dish is slowly moved into constant incubator, cultivate 18h for 37 DEG C, take out and observe each culture dish colony growth situation, and measure antibacterial circle diameter with slide gauge, record data, concentration for sample is respectively 5mg/ml, 10mg/ml, 15mg/ml, 20mg/ml.
2, result
Measured by above method, result is as shown in table 1.
Table 1
。
3, conclusion
The main several pathogenic bacterium of Herba Sophorae alopecuroidis total alkali to bovine mastitis and endometritis all have inhibitory action, and the rising inhibitory action along with Herba Sophorae alopecuroidis total alkali concentration in certain scope also progressively strengthens, wherein the strongest to the staphylococcic inhibitory action of epidermis, the most weak relative to other three kinds of bacterium to the inhibitory action of streptococcus agalactiae.The inhibitory action of Herba Sophorae alopecuroidis total alkali to other antibacterial can be studied further, for the exploitation of Herba Sophorae alopecuroidis total alkali provide reference frame.
(2) Herba Sophorae alopecuroidis total alkali is studied acute toxicity test in mice:
The acute toxicity adopting improvement karber's method mensuration Herba Sophorae alopecuroidis total alkali to inject mouse peritoneal, evaluates its safety, for data for clinical drug use provides foundation.During test, to the Herba Sophorae alopecuroidis total alkali of test group mouse gavaging variable concentrations, observe activity and the death condition of mice after administration, adopt improvement Kou Shi formulae discovery LD
50and LD
5095% fiducial limit, result shows, Herba Sophorae alopecuroidis total alkali is through the LD of lumbar injection
50for 559.24mg/kg, LD
5095% be crediblely limited to 471.95mg/kg ~ 663.59ma/kg.Result of the test is tentatively pointed out, and the toxicity of Herba Sophorae alopecuroidis total alkali is lower, and data for clinical drug use is reliable.
1, materials and methods
1.1 material
1.1.1 experimental animal
Kunming system cleaning grade healthy mice, male and female half and half, body weight (20 ± 2) g, purchased from Lanzhou University's experimental animal center (sweet doctor moves word 14-005).
1.1.2 test medicine
Herba Sophorae alopecuroidis total alkali, extracts preparation by Hinapharm Pharmaceutical Co., Ltd..
1.2 method
1.2.1 the extraction of Herba Sophorae alopecuroidis total alkali
Get Herba Sophorae alopecuroidis seed to pulverize, add ethanol water and carry out ultrasonic extraction, after filtration, reclaim ethanol, add 2%HCl and adjust PH, add washed with diethylether 2 times, obtain filtrate precipitation.Add chloroform extraction again, reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali.
1.2.2 trial test
Before trial test, mice is carried out to the nursing observation of 7d, in the observation period, mice free choice feeding, drinking-water, eliminates natural death mice.Choose healthy mice 25 in the trial test phase, male and female half and half, be divided into 5 groups at random.Often organize 5, before test, fasting 8 hours, is mixed with 5 concentration by Herba Sophorae alopecuroidis total alkali, and each group isoconcentration intraperitoneal injection, determines the dosage range of formal test.
1.2.3 formal test
According to trial test, determine Dn and Dm, between Dn and Dm, establish 6 dosage groups, often organize 10 mices, male and female half and half.Before test, fasting 8 hours, freely drinks water.Observe and record behavior state and the death condition of mice after intraperitoneal injection immediately, the observation period is 7 days, and experimentally result calculates median lethal dose(LD 50) (LD
50), dissect in time dead mice, record pathological changes situation, observes the pathological change of mouse peritoneal and parenchymatous organ.
1.2.4 LD
50computational methods
Improvement karber's method is adopted to calculate LD
50and LD
5095% fiducial limit, computing formula is as follows:
LD
5095% fiducial limit=lg
-1(lgLD
50± 1.96 × Sx
50)
In formula: i is group distance, the i.e. difference of the log10 dose of adjacent 2 groups of dosage; Xm is maximum mortality rate group dosage logarithm; Σ p is each dosage group mortality rate sum; P is each dosage group mortality rate; Q is each dosage group survival rate; N is each treated animal number; Sx
50for lgLD
50standard error.
2, results and analysis
2.1 trial test results
Herba Sophorae alopecuroidis total alkali is as shown in table 2 to Mouse Acute Toxicity trial test result.Drawn by trial test result, Dn and Dm is respectively 200mg/kg and 1000mg/kg.Be divided into 6 dosage groups according to trial test result determination formal test, obtain dose ratio by following formula:
In formula, r is the ratio of two adjacent groups dosage; N is formal test required group number.Calculate the r=0.707 of formal test.
Table 2
。
According to trial test result, be set to 200mg/kg, 276.24mg/kg, 381.55mg/kg, 527.00mg/kg, 727.90mg/kg, 1000mg/kg body weight 6 dosage groups, carry out acute toxicity test.Result is as shown in table 3.According to the data in table 3, calculated by formula, Herba Sophorae alopecuroidis total alkali is to the median lethal dose(LD 50) LD of mice
50=559.24mg/kg, LD
5095% be crediblely limited to 471.95mg/kg ~ 663.59ma/kg.Therefore Herba Sophorae alopecuroidis total alkali is judged to mice through lumbar injection LD
50be greater than 500mg/kg, according to China's animal toxicology acute toxicity staging, Herba Sophorae alopecuroidis total alkali belongs to low toxicity material.
Table 3
。
2.2, Herba Sophorae alopecuroidis total alkali is to acute toxicity test in mice clinical observation:
Mice disposable celiac drug administration by injection, when dosage is 200mg/kg, within experimental period, mice is without the phenomena of mortality, and within 2 hours, not dead mice symptom alleviates gradually, and after 12 hours, basic recovery normally, can normally be taken food and drink water; When dosage is 276.24mg/kg, dead mouse one in 1 hour; When dosage is 381.55mg/kg, dead mouse 2 within 15 minutes; When dosage is 527.00mg/kg, dead mouse 3 in 10 minutes; When dosage is 727.90mg/kg, dead mouse 6 in 10 minutes; When dosage is 1000mg/kg, in 5 minutes, mice is all dead.
The main poisoning symptom of mice after intraperitoneal injection shows as lethargy, hogback, and whole body is twitched, and fore paw scratches mouth, and has discontinuity excited.On one's deathbed present jump action before, extremity are in swimming shape then more, dead subsequently.
2.3, the dead mice of acute toxicity test cuts open inspection result
Dissect dead mice and find there is a small amount of hydrops in abdominal cavity, heart color and luster is dim, and liver has slight enlargement, and kidney is observed without significant change, and other organs is without obviously observing pathological changes.Within 6 days, put to death afterwards and anatomic observation survival mice and blank group mice, do not find seroperitoneum, other organs is without obviously observing pathological changes.
3, result
Result of the test shows, the median lethal dose(LD 50) LD that Herba Sophorae alopecuroidis total alkali is injected mouse peritoneal
50for 559.24mg/kg, LD
5095% be crediblely limited to 471.95mg/kg ~ 663.59ma/kg, meet low toxicity material grade scale.
After disposable mouse peritoneal drug administration by injection, survival mice is basic after 12 hours recovers normal, and carry out Anatomical Observation to survival mice after 6 days, each internal organs are showed no obviously observes pathological changes.After test is described, survival mice recovers very fast, and medicine is less to its long lasting effect.
The research and apply of Herba Sophorae alopecuroidis total alkali on human clinical medicine is more, but the research and apply in Animal husbandry production is less, this is tested by evaluating the acute toxicity of Herba Sophorae alopecuroidis total alkali, for the further developmental research of later Herba Sophorae alopecuroidis total alkali in animal husbandry should be used as a preparation.
(3) Herba Sophorae alopecuroidis total alkali infusion liquid treatment bovine mastitis clinical trial:
Herba Sophorae alopecuroidis total alkali infusion liquid treatment bovine mastitis and endometritis, definite effect, not easily rebounds after healing.
From 2011 to 2012 years to the clinical trial of Herba Sophorae alopecuroidis total alkali infusion liquid treatment bovine mastitis.Lanzhou Duan Jia is successively selected to spread out 18 milch cows suffering from mastitis of cattle farm as subjects through consultation.These 14 ill milch cow clinical symptoms main manifestations are: milk production of cow declines, and breast portion swelling is rubescent, has heat and pain, has thin slice, floccule, pus block or blood in milk, and milk is rare water sample or squeezes not get off.4 are seriously ill, and its breast shows unexpected swelling, heating, hardening, newborn district are responsive, even show that body temperature raises, diet subtracts greatly or useless absolutely, down in spirits etc., look into according to clinical symptoms, Breast palpation and newborn quality inspection and be defined as clinical mastitis.
1. test medicine
Herba Sophorae alopecuroidis total alkali infusion liquid: Guang Donghai Na Chuan pharmaceutcal corporation, Ltd provides, lot number is 20110218,100ml/ bottle.
Ampicillin Sodium For Injection is purchased from local veterinary drug shop, and by expedition, pharmaceutcal corporation, Ltd produces, and lot number is 20120308, and specification is that 5g/ props up.
2. test
2.1 test groupings
5 to 19 June in 2011, occur that the ill milch cow of clinical symptoms is as first group in this cattle farm using 5; There are 22 ill milch cows occurring clinical symptoms on June 25 to July 25 in same pasture, select 18 with the ill milch cow of first group of situation basic simlarity as second group; To two groups, administration Herba Sophorae alopecuroidis total alkali infusion liquid, injection ampicillin are treated respectively respectively.
2.2 test method
Test group adopts Herba Sophorae alopecuroidis total alkali infusion liquid to treat, every cattle potion, breast perfusion, every day 2 times, each 50ml, and being used in conjunction 5d is a course for the treatment of; Matched group adopts Ampicillin Sodium For Injection, and breast perfusion, the ill each 5g in newborn room, once a day, being used in conjunction 5d is a course for the treatment of.
3 results
Cure after medication and show as red, swollen, hot, pain and hard transference cure, perusal milk sample normally, milk yield recovery.The cure rate for the treatment of mammitis of cow by Herba Sophorae alopecuroidis total alkali infusion liquid is 88.8%, and the cure rate of ampicillin treatment mammitis of cow is 77.7%.
18 cow heads of Herba Sophorae alopecuroidis total alkali infusion liquid group have 16 mastitis transference cures or obviously change after medication 5d, and cure rate is 88.8%(16/18).9 cow heads of ampicillin group have 7 mastitis transference cures or obviously change after medication after five days, cure rate is 77.7%(7/9).The therapeutic effect of Herba Sophorae alopecuroidis total alkali infusion liquid group is higher than ampicillin group.
Accompanying drawing explanation
Fig. 1 is Herba Sophorae alopecuroidis total alkali concentration when being 5mg/ml and 20mg/ml, to colibacillary antibacterial figure.
Fig. 2 is Herba Sophorae alopecuroidis total alkali concentration when being 5mg/ml and 20mg/ml, to the antibacterial figure of staphylococcus aureus.
Fig. 3 is Herba Sophorae alopecuroidis total alkali concentration when being 5mg/ml and 20mg/ml, to the staphylococcic antibacterial figure of epidermis.
Fig. 4 is Herba Sophorae alopecuroidis total alkali concentration when being 5mg/ml and 20mg/ml, to the antibacterial figure of streptococcus agalactiae.
Detailed description of the invention
embodiment 1:
The preparation method of the Chinese medicinal perfusion liquid containing Herba Sophorae alopecuroidis total alkali, comprises the following steps:
1) get Herba Sophorae alopecuroidis seed to pulverize, add ethanol water and carry out ultrasonic extraction, reclaim ethanol after filtration, add 2% hydrochloric acid and adjust PH, add washed with diethylether 2 times, obtain filtrate precipitation, then add chloroform extraction precipitation, reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali;
2) add water for injection in the Herba Sophorae alopecuroidis total alkali 3g obtained in step 1) and be settled to 100ml, adopt flowing steam sterilization 30min, aseptically carry out fill and obtain finished product Chinese medicinal perfusion liquid.
embodiment 2:
The preparation method of the Chinese medicinal perfusion liquid containing Herba Sophorae alopecuroidis total alkali, comprises the following steps:
1) get Herba Sophorae alopecuroidis seed to pulverize, add ethanol water and carry out ultrasonic extraction, reclaim ethanol after filtration, add 2% hydrochloric acid and adjust PH, add washed with diethylether 2 times, obtain filtrate precipitation, then add chloroform extraction precipitation, reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali;
2) add water for injection in the Herba Sophorae alopecuroidis total alkali 8g obtained in step 1) and be settled to 100ml, adopt flowing steam sterilization 40min, aseptically carry out fill and obtain finished product Chinese medicinal perfusion liquid.
embodiment 3:
The preparation method of the Chinese medicinal perfusion liquid containing Herba Sophorae alopecuroidis total alkali, comprises the following steps:
1) get Herba Sophorae alopecuroidis seed to pulverize, add ethanol water and carry out ultrasonic extraction, reclaim ethanol after filtration, add 2% hydrochloric acid and adjust PH, add washed with diethylether 2 times, obtain filtrate precipitation, then add chloroform extraction precipitation, reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali;
2) add water for injection in the Herba Sophorae alopecuroidis total alkali 6g obtained in step 1) and be settled to 100ml, adopt flowing steam sterilization 35min, aseptically carry out fill and obtain finished product Chinese medicinal perfusion liquid.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (1)
1. the application of Herba Sophorae alopecuroidis total alkali in the Chinese medicinal perfusion liquid for the preparation for the treatment of bovine mastitis and endometritis, it is characterized in that, the preparation method of described Chinese medicinal perfusion liquid, comprises the following steps:
1) get Herba Sophorae alopecuroidis seed meal and be broken to 50-80 order, adding percent by volume is that the ethanol water of 50%-90% carries out ultrasonic extraction, supersonic frequency 20KHz, 5000W, reclaims ethanol after filtration, adds 2% hydrochloric acid and adjusts pH to be 2-5, add washed with diethylether 2 times, obtain filtrate precipitation, then add chloroform extraction three precipitations, reclaim under reduced pressure chloroform obtains Herba Sophorae alopecuroidis total alkali;
2) add water for injection standardize solution in the Herba Sophorae alopecuroidis total alkali obtained in step 1), the mass ratio of Herba Sophorae alopecuroidis total alkali and water for injection is 6:94, adopts flowing steam sterilization 30-40min, aseptically carries out fill and obtain finished product Chinese medicinal perfusion liquid; Containing Herba Sophorae alopecuroidis total alkali 6g in Chinese medicinal perfusion liquid; The use amount of described Chinese medicinal perfusion liquid is each newborn district 30-60ml of every cow head.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310079272.0A CN103142683B (en) | 2013-03-12 | 2013-03-12 | Be used for the treatment of Chinese medicinal perfusion liquid containing Herba Sophorae alopecuroidis total alkali of bovine mastitis and endometritis and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310079272.0A CN103142683B (en) | 2013-03-12 | 2013-03-12 | Be used for the treatment of Chinese medicinal perfusion liquid containing Herba Sophorae alopecuroidis total alkali of bovine mastitis and endometritis and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103142683A CN103142683A (en) | 2013-06-12 |
| CN103142683B true CN103142683B (en) | 2015-09-09 |
Family
ID=48541192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310079272.0A Active CN103142683B (en) | 2013-03-12 | 2013-03-12 | Be used for the treatment of Chinese medicinal perfusion liquid containing Herba Sophorae alopecuroidis total alkali of bovine mastitis and endometritis and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103142683B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2700081C1 (en) * | 2019-01-14 | 2019-09-12 | Общество с ограниченной ответственностью "Агровейт" | Preparation "berkana" for prevention and treatment of endometritis in cows and a method for production thereof |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103271983B (en) * | 2013-05-17 | 2016-05-18 | 广东海纳川生物科技股份有限公司 | A kind of endometritis and colpitic perfusion liquid, its preparation method and application for the treatment of pig |
| CN104208136A (en) * | 2014-09-25 | 2014-12-17 | 宁夏智弘生物科技有限公司 | Preparation process and using method of sophora alopecuroide alkaloid dairy cow breast injectant |
| CN112206250A (en) * | 2020-10-26 | 2021-01-12 | 宁夏职业技术学院（宁夏广播电视大学） | Application of sophora alopecuroide extract in preparation of drugs or bacteriostatic agents for treating drug-resistant diseases of poultry |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102580068A (en) * | 2012-03-20 | 2012-07-18 | 宁夏职业技术学院 | Compound perfusate for treating mammitis of dairy cattle and preparation method thereof |
| CN102793742A (en) * | 2012-09-11 | 2012-11-28 | 冷晓红 | Film coating agent for disinfecting dairy cow breasts and preparation method of film coating agent |
-
2013
- 2013-03-12 CN CN201310079272.0A patent/CN103142683B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102580068A (en) * | 2012-03-20 | 2012-07-18 | 宁夏职业技术学院 | Compound perfusate for treating mammitis of dairy cattle and preparation method thereof |
| CN102793742A (en) * | 2012-09-11 | 2012-11-28 | 冷晓红 | Film coating agent for disinfecting dairy cow breasts and preparation method of film coating agent |
Non-Patent Citations (5)
| Title |
|---|
| 刘军锋,等.苦豆子生物碱抗菌活性的测定.《北京化工大学学报》.2011,第38卷(第2期),第84-88页. * |
| 张为民,等.苦豆子生物碱抑菌抗炎作用研究.《动物医学进展》.2005,第26卷(第10期),第82-85页. * |
| 木合布力·阿布力孜,等.新疆苦豆子有效成分氧化苦参碱的提取分离及初步鉴定研究.《新疆医科大学学报》.2010,第23卷(第11期),第1314-1316页. * |
| 杨巧丽,等.中药苦豆子的研究进展.《西北药学杂志》.2011,第26卷(第3期),第232-236页. * |
| 超声波提取苦豆子生物碱的工艺研究;余永婷;《中国食品添加剂》;20081231;第69-72、第57页、第3.4、3.5节 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2700081C1 (en) * | 2019-01-14 | 2019-09-12 | Общество с ограниченной ответственностью "Агровейт" | Preparation "berkana" for prevention and treatment of endometritis in cows and a method for production thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103142683A (en) | 2013-06-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102114044B (en) | Artificially processed bear bile powder and preparation method thereof | |
| WO2019149156A1 (en) | Uses of pulsatilla chinensis extract in preparing drug for treating viral and/or bacterial diseases | |
| CN104288356B (en) | Treat traditional Chinese medicine infusion solution of mastitis for milk cows and preparation method thereof | |
| CN102961469B (en) | Traditional Chinese medicine dispersible tablet for treating upper respiratory infection, and preparation method and quality detection method thereof | |
| KR20110132407A (en) | Plant extract compositions for prevention and treatment of influenza | |
| CN103142683B (en) | Be used for the treatment of Chinese medicinal perfusion liquid containing Herba Sophorae alopecuroidis total alkali of bovine mastitis and endometritis and preparation method thereof | |
| CN101869656B (en) | Chinese medicinal preparation for treating cough with asthma and preparation method thereof | |
| WO2011035734A1 (en) | Relinqing extract, preparative method and use thereof | |
| CN106511512A (en) | Traditional Chinese medicine external-use gel and preparation method and application thereof | |
| CN102085248B (en) | Traditional Chinese medicinal composition for treating cervix diseases, method for preparing same and method for detecting same | |
| CN102526573A (en) | Medicament for treating child cold | |
| CN101254270B (en) | External use traditional Chinese medicine preparation for curing gynecology inflammation and method of preparing the same | |
| CN106214740A (en) | A kind of Chinese medicine composition and its production and use | |
| CN1762967B (en) | Enoxolone derivative, preparation method and uses | |
| CN105726631A (en) | Application of officinal magnolia bark extract to prepare medicine for treating cow mastitis | |
| CN106177221B (en) | Traditional Chinese medicine composition for treating porcine colibacillosis diarrhea and preparation method thereof | |
| CN106267193A (en) | A kind of for infusion of medicine agent treating bovine mastitis and its preparation method and application | |
| CN103271983B (en) | A kind of endometritis and colpitic perfusion liquid, its preparation method and application for the treatment of pig | |
| CN101816762A (en) | Medicinal preparation for treating colpitis and preparation method thereof | |
| CN1506092A (en) | Elsholizia extract capsule for treating diarrhea and its prepn | |
| CN102258620B (en) | Chinese medicinal injection for treating mixed infection of swine streptococcus and swine fever and preparation method thereof | |
| CN106511497A (en) | Traditional Chinese medicinal composition and preparation method thereof | |
| CN102293959B (en) | Anticancer traditional Chinese medicine and preparation method thereof | |
| CN109470788A (en) | A kind of method of quality control of FUKE QIANJIN PIAN | |
| CN104622987B (en) | A kind of pharmaceutical composition for treating chronic hepatitis liver cirrhosis and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent for invention or patent application | ||
| CB02 | Change of applicant information |
Address after: 528515 Guangdong province Foshan Gaoming Cangjiang Industrial Park West Sand Water District Heyuan Yang Road Applicant after: GUANGDONG HINABIOTECH CO., LTD. Address before: 528515 Guangdong province Foshan Gaoming Cangjiang Industrial Park West Road Guangdong District Yang sand water Heyuan Hainachuan pharmaceutical Limited by Share Ltd Applicant before: Hinapharm Pharmaceutical Co., Ltd. |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: HINAPHARM PHARMACEUTICAL CO., LTD. TO: GUANGDONG HINAPHARM BIOTECHNOLOGY CO., LTD. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |